CmaCh04G012500 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G012500
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionFerric reduction oxidase
LocationCma_Chr04: 6407403 .. 6410961 (-)
RNA-Seq ExpressionCmaCh04G012500
SyntenyCmaCh04G012500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCCTATAAATATCATTCCTTTCACTCAAATTTCTATATTACTTTGATTGAAAGAAAAAACCCCAAGTTGTGAAGAAACTATGGGAGTTTTGAGCTTAGCCATTAGGGTTTTGGTGTTCATCTTGTTTGTGGGGTGGCTTTTTATTTGGTTCATGATGCCCACTAATACTTATAGACATCAATGGCTTCCCAAAATTCAGGACAAGAGTCGTAGCTCGTCGTATTTTGGATCCGAAGGTTTTGTTTTTCTTTTTTGTGTAATTTTTAAGTTTGATGTAGAGTTTTTACACGGTTGTTTTGCTTTGGCACAGGAACTTCTCTTTTGATGTATACGTTCCCCATTTTGTTCATTGCTGTTTTGGGATGTGTTTATGTTCATATTGAACGAAGATGCAGAGAACAAAACGTGAAAATTAGGTGAATAGTTATGTTGTATAGAGGTTGTTTTGTATCGTTCTTATTTAAATGAGTTTTGTTTTGATCTTGATAGGAATAGAGGAAACGATAATGGTTTGGCGATGTGGAAGAGGCCAGGGTTTGTGAAAGGGCCACTCGGGATTGTGTCGTGGACAGAACTAGCGTTGCTGGCCATGTTCGTGGCACTTCTAGTTTGGTCATTTGCTACATATTTAAACAATAGCTACCACAGCGAGAATGAGTAAGAACAACTATATGCAATAGTTGAAGTTCTAAGTAATTAATCAACTAACAACTTTGATACTGATGGCAGGTGGGAAAGTAGATTGGGAACTGCAGCGTATTGGCTTGGAGTTGTTGGGAATATTTGTTTGGTGTTTTTGTTCTTTCCGGTGGCACGCGGGTCATCGCTACTGCCGTTGCTCGGCCTTACATCGGAGAGTAGCATTAAGTATCATATATGGCTCGGTCATATGACGATGGCGTTCTTCTCCGCTCATGGAATCTGCTTCGTTGTCTATTGGGCTGCTACTCATAATCTTTCTCAAGTGAGTATTCATTATAACATGTGATATCCCATGTCGGTTGGAGAGGGGAACTGAACATTTCTTATGAGAGTGTGGAAACCTCTCCCAATCATGTGACTAACGACTATACGTAACGGCGAAAGCATACAACTGTTGCAAATGGTATAAGAGCCAAACATTAGGTTGTGTGCCAGCGAGGACGCTCCCCTCCTTAGGAGAGTGGATTGTGAGATTCCACTTCGATTGGAGAGGGCAACAAAACATTCCTTATAGGGTGTGGAAACCTCTCCCTATTCCGTAATAGATACATTTTTGAAACCGCGGGGCTACTCTTTTTGTTTTGGCAGATGCTTGAATGGGCTAAAACAGATGTATCAAACGTAGCAGGAGAGGTATCACTAGTGTGTGGACTCATAATATGGGCAACAACATTCCCTGGCGTTCGAAGGAAGTTCTTCGAGCTCTTCCTCTACACTCACCATCTCTACATTCTCTTCATCGTCTTCTTCATCTTCCACGTCGGCATCTCCTACGCCTGCATTATGCTCCCTGGTTTCTACCTCTTCCTCCTCGACCGCTTCCTCCGCTTCCTCCAGTCCCGCCGCCGTGTTCGCCTCCTCTCTGCTCGCCTCCTCCCCTGCCACACACTCGAGCTCAACTTCTCCAAACACCCAGGTTCATCAAATCCTCCATTTCCCCCTTCTTTTACCACCAAAACCCACATGGGTTCTCAACATTTTCTTGATTTTTTTTGTAGGATTGAAGTACACCCCAACGAGCACTCTGTTCCTGAATATCCCCTGCATCTCGAAGCTCCAATGGCACCCGTTTACCGTCACATCCCACGCCGACTTAGAACCAGAGACACTCTCCGTCGTCATCAAATGCGAGGGAACATGGTCCACCAAGCTTTACCAGACGCTTTCTTCGCCATCCGCTATTGATAACCACCTCCAAGTCTCCCTCGAAGGCCCCTACGGCCCTGTTTCCACTTCCTTCCTCCAATTCGACTCTCTTATCATGATCAGCGGCGGCAGTGGCATAACCCCTTTCATTTCCATAATCAGACACCTCATTCATAACTCCAATTCCCATCAACAAATCCCCAAACTCCTCCTTATCTCCGCCTTCAAAAACTCCTCCGATTTGACCTTCCTCCACCTCCTCCTCCCTCTCTCTGGCGCCAACACTCTGGAGAATTTCCAAATCGAGGCCTATGTGACGAGAGACAAATCCCCTAAAGACGAAAACCCACAATTCAGAACGGTGATTTTCAAACCCCATGAAGGGGATTCCCCTGCGACGGCGATTTTAGGGCCCAATGGATGGCTATGGCTAGCGGGGATTATCTGTTCGTCGTTTGGGATTTTCTTGGCTCTAATGGGGATTGTGACAAGATATTACATATACCCAATTGATGGGAATTCAAACGATGTGTTCAATTTGAGTTTAAGGTCGTTTTTACACATGTTGGGGCTGTGTTTATGCATTGTCATGGCGGCGACTGCGGCGGTGATTTGGAACAAGAGGAAACTAGCGAAGGAAGGCCGGCAGATTCAGAACGTCGAAGGGGCTACGCCAAATGCGTCTCCATCGGCAATGGGGTTTGACAACGTTGGCGAGATGGAATTGGAGAGCGTTCCATTTGAAGCTCTTGCACAATCTGTTCATGTTTATTATGGGGAAAGGCCCAACCTTCCAAGTAAGGCTTCCATCAAAATTTTCAAAAACGGTTTGTTGGATTAAAAGAAAAGTCCCCCTCGACTAATTTTTTAGAGAATGATCATAGTTTTATGATCAACTAATACTCTCTCCATTGATTTGAGGCCTTTTAGGAAGCCCAAAGCAAAGTCACGAAAGTTTATGTTCAGAGTGGACAATATCATACCATTGTGGATATTAGTATTCGTCTAACATAATATCAGAGTCATGCCCTAAACTTAGTTGTGCCAATAGATTGGTAAATCCTCGAATGTCGAACAAAGGATTCCAAAAGAAAAGGAATCAAGCCTTCTCGAAGGCATAGTCAAAAAAAGGACTAAGACTCCAAAAAAAGGAGTCTAGCCTCGATTAAGAGGAGGCGTGTTGGATGAAAAGAAAAGTTCCACATCGGCTAATTTAGGGAATGATCATGCGTTTATAATCAAGGAATACTCTCACCATTGGTTTGAGAGCTTATGCTCAAAGTGAACAATATCATATCATTGTGGAGAGTCGTGTTCGTCTAACATGGTTCATGTGTGTGTCTAAAATTTCTTCTTTTTTTTTTTAGGAATTTTACTAGAACGCAAAGGCGAAAACGTGGGAGTTCTTGCATCAGGCCCCAAGAAGATGAGGCAAGAAGTTGCAGCCATTTGCGGCTCTGGTTTGGCTCCAAATCTCCATTTTCAGTCCATCAGCTTCACTTGGTGAACAATAATCCAATACCCATAACAATGTTCCAATCATGTGTTTTTGTTTTTGTGTTTTTGTGTTTTTTTTTTTTTTTCTTGGTCTTTTGTGTGTTAAGTGAGGAAGAAAGGTCTGATTTAGTTTGTTTTTAGTTTGTTTCATTCTGTGTAAGCATTTAAATCGTTTCATGAAAACGCGTTTCGGATTATGTTCCATACGGCAG

mRNA sequence

CTCCCTATAAATATCATTCCTTTCACTCAAATTTCTATATTACTTTGATTGAAAGAAAAAACCCCAAGTTGTGAAGAAACTATGGGAGTTTTGAGCTTAGCCATTAGGGTTTTGGTGTTCATCTTGTTTGTGGGGTGGCTTTTTATTTGGTTCATGATGCCCACTAATACTTATAGACATCAATGGCTTCCCAAAATTCAGGACAAGAGTCGTAGCTCGTCGTATTTTGGATCCGAAGGAACTTCTCTTTTGATGTATACGTTCCCCATTTTGTTCATTGCTGTTTTGGGATGTGTTTATGTTCATATTGAACGAAGATGCAGAGAACAAAACGTGAAAATTAGGAATAGAGGAAACGATAATGGTTTGGCGATGTGGAAGAGGCCAGGGTTTGTGAAAGGGCCACTCGGGATTGTGTCGTGGACAGAACTAGCGTTGCTGGCCATGTTCGTGGCACTTCTAGTTTGGTCATTTGCTACATATTTAAACAATAGCTACCACAGCGAGAATGAGTGGGAAAGTAGATTGGGAACTGCAGCGTATTGGCTTGGAGTTGTTGGGAATATTTGTTTGGTGTTTTTGTTCTTTCCGGTGGCACGCGGGTCATCGCTACTGCCGTTGCTCGGCCTTACATCGGAGAGTAGCATTAAGTATCATATATGGCTCGGTCATATGACGATGGCGTTCTTCTCCGCTCATGGAATCTGCTTCGTTGTCTATTGGGCTGCTACTCATAATCTTTCTCAAATGCTTGAATGGGCTAAAACAGATGTATCAAACGTAGCAGGAGAGGTATCACTAGTGTGTGGACTCATAATATGGGCAACAACATTCCCTGGCGTTCGAAGGAAGTTCTTCGAGCTCTTCCTCTACACTCACCATCTCTACATTCTCTTCATCGTCTTCTTCATCTTCCACGTCGGCATCTCCTACGCCTGCATTATGCTCCCTGGTTTCTACCTCTTCCTCCTCGACCGCTTCCTCCGCTTCCTCCAGTCCCGCCGCCGTGTTCGCCTCCTCTCTGCTCGCCTCCTCCCCTGCCACACACTCGAGCTCAACTTCTCCAAACACCCAGGATTGAAGTACACCCCAACGAGCACTCTGTTCCTGAATATCCCCTGCATCTCGAAGCTCCAATGGCACCCGTTTACCGTCACATCCCACGCCGACTTAGAACCAGAGACACTCTCCGTCGTCATCAAATGCGAGGGAACATGGTCCACCAAGCTTTACCAGACGCTTTCTTCGCCATCCGCTATTGATAACCACCTCCAAGTCTCCCTCGAAGGCCCCTACGGCCCTGTTTCCACTTCCTTCCTCCAATTCGACTCTCTTATCATGATCAGCGGCGGCAGTGGCATAACCCCTTTCATTTCCATAATCAGACACCTCATTCATAACTCCAATTCCCATCAACAAATCCCCAAACTCCTCCTTATCTCCGCCTTCAAAAACTCCTCCGATTTGACCTTCCTCCACCTCCTCCTCCCTCTCTCTGGCGCCAACACTCTGGAGAATTTCCAAATCGAGGCCTATGTGACGAGAGACAAATCCCCTAAAGACGAAAACCCACAATTCAGAACGGTGATTTTCAAACCCCATGAAGGGGATTCCCCTGCGACGGCGATTTTAGGGCCCAATGGATGGCTATGGCTAGCGGGGATTATCTGTTCGTCGTTTGGGATTTTCTTGGCTCTAATGGGGATTGTGACAAGATATTACATATACCCAATTGATGGGAATTCAAACGATGTGTTCAATTTGAGTTTAAGGTCGTTTTTACACATGTTGGGGCTGTGTTTATGCATTGTCATGGCGGCGACTGCGGCGGTGATTTGGAACAAGAGGAAACTAGCGAAGGAAGGCCGGCAGATTCAGAACGTCGAAGGGGCTACGCCAAATGCGTCTCCATCGGCAATGGGGTTTGACAACGTTGGCGAGATGGAATTGGAGAGCGTTCCATTTGAAGCTCTTGCACAATCTGTTCATGTTTATTATGGGGAAAGGCCCAACCTTCCAAGAATTTTACTAGAACGCAAAGGCGAAAACGTGGGAGTTCTTGCATCAGGCCCCAAGAAGATGAGGCAAGAAGTTGCAGCCATTTGCGGCTCTGGTTTGGCTCCAAATCTCCATTTTCAGTCCATCAGCTTCACTTGGTGAACAATAATCCAATACCCATAACAATGTTCCAATCATGTGTTTTTGTTTTTGTGTTTTTGTGTTTTTTTTTTTTTTTCTTGGTCTTTTGTGTGTTAAGTGAGGAAGAAAGGTCTGATTTAGTTTGTTTTTAGTTTGTTTCATTCTGTGTAAGCATTTAAATCGTTTCATGAAAACGCGTTTCGGATTATGTTCCATACGGCAG

Coding sequence (CDS)

ATGGGAGTTTTGAGCTTAGCCATTAGGGTTTTGGTGTTCATCTTGTTTGTGGGGTGGCTTTTTATTTGGTTCATGATGCCCACTAATACTTATAGACATCAATGGCTTCCCAAAATTCAGGACAAGAGTCGTAGCTCGTCGTATTTTGGATCCGAAGGAACTTCTCTTTTGATGTATACGTTCCCCATTTTGTTCATTGCTGTTTTGGGATGTGTTTATGTTCATATTGAACGAAGATGCAGAGAACAAAACGTGAAAATTAGGAATAGAGGAAACGATAATGGTTTGGCGATGTGGAAGAGGCCAGGGTTTGTGAAAGGGCCACTCGGGATTGTGTCGTGGACAGAACTAGCGTTGCTGGCCATGTTCGTGGCACTTCTAGTTTGGTCATTTGCTACATATTTAAACAATAGCTACCACAGCGAGAATGAGTGGGAAAGTAGATTGGGAACTGCAGCGTATTGGCTTGGAGTTGTTGGGAATATTTGTTTGGTGTTTTTGTTCTTTCCGGTGGCACGCGGGTCATCGCTACTGCCGTTGCTCGGCCTTACATCGGAGAGTAGCATTAAGTATCATATATGGCTCGGTCATATGACGATGGCGTTCTTCTCCGCTCATGGAATCTGCTTCGTTGTCTATTGGGCTGCTACTCATAATCTTTCTCAAATGCTTGAATGGGCTAAAACAGATGTATCAAACGTAGCAGGAGAGGTATCACTAGTGTGTGGACTCATAATATGGGCAACAACATTCCCTGGCGTTCGAAGGAAGTTCTTCGAGCTCTTCCTCTACACTCACCATCTCTACATTCTCTTCATCGTCTTCTTCATCTTCCACGTCGGCATCTCCTACGCCTGCATTATGCTCCCTGGTTTCTACCTCTTCCTCCTCGACCGCTTCCTCCGCTTCCTCCAGTCCCGCCGCCGTGTTCGCCTCCTCTCTGCTCGCCTCCTCCCCTGCCACACACTCGAGCTCAACTTCTCCAAACACCCAGGATTGAAGTACACCCCAACGAGCACTCTGTTCCTGAATATCCCCTGCATCTCGAAGCTCCAATGGCACCCGTTTACCGTCACATCCCACGCCGACTTAGAACCAGAGACACTCTCCGTCGTCATCAAATGCGAGGGAACATGGTCCACCAAGCTTTACCAGACGCTTTCTTCGCCATCCGCTATTGATAACCACCTCCAAGTCTCCCTCGAAGGCCCCTACGGCCCTGTTTCCACTTCCTTCCTCCAATTCGACTCTCTTATCATGATCAGCGGCGGCAGTGGCATAACCCCTTTCATTTCCATAATCAGACACCTCATTCATAACTCCAATTCCCATCAACAAATCCCCAAACTCCTCCTTATCTCCGCCTTCAAAAACTCCTCCGATTTGACCTTCCTCCACCTCCTCCTCCCTCTCTCTGGCGCCAACACTCTGGAGAATTTCCAAATCGAGGCCTATGTGACGAGAGACAAATCCCCTAAAGACGAAAACCCACAATTCAGAACGGTGATTTTCAAACCCCATGAAGGGGATTCCCCTGCGACGGCGATTTTAGGGCCCAATGGATGGCTATGGCTAGCGGGGATTATCTGTTCGTCGTTTGGGATTTTCTTGGCTCTAATGGGGATTGTGACAAGATATTACATATACCCAATTGATGGGAATTCAAACGATGTGTTCAATTTGAGTTTAAGGTCGTTTTTACACATGTTGGGGCTGTGTTTATGCATTGTCATGGCGGCGACTGCGGCGGTGATTTGGAACAAGAGGAAACTAGCGAAGGAAGGCCGGCAGATTCAGAACGTCGAAGGGGCTACGCCAAATGCGTCTCCATCGGCAATGGGGTTTGACAACGTTGGCGAGATGGAATTGGAGAGCGTTCCATTTGAAGCTCTTGCACAATCTGTTCATGTTTATTATGGGGAAAGGCCCAACCTTCCAAGAATTTTACTAGAACGCAAAGGCGAAAACGTGGGAGTTCTTGCATCAGGCCCCAAGAAGATGAGGCAAGAAGTTGCAGCCATTTGCGGCTCTGGTTTGGCTCCAAATCTCCATTTTCAGTCCATCAGCTTCACTTGGTGA

Protein sequence

MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALLAMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIMISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENFQIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Homology
BLAST of CmaCh04G012500 vs. ExPASy Swiss-Prot
Match: P92949 (Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2)

HSP 1 Score: 763.8 bits (1971), Expect = 1.6e-219
Identity = 382/706 (54.11%), Postives = 522/706 (73.94%), Query Frame = 0

Query: 9   RVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFPILFIAV 68
           + L+ ++F+G + +W MMPT TYR +WLP ++ K  +S+YFG+ GT+L MY FP++ +A 
Sbjct: 28  KFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFPMMVVAC 87

Query: 69  LGCVYVHIERRCREQNVKIRNRGNDNGLAMW---KRPGFVKGPLGIVSWTELALLAMFVA 128
           LGCVY+H + R    ++    +G      +W   ++P  VKGPLGIVS TE+  LAMFVA
Sbjct: 88  LGCVYLHFKNRKSPHHIDRETKG-----GVWSKLRKPMLVKGPLGIVSVTEITFLAMFVA 147

Query: 129 LLVWSFATYLNNSY----------HSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGS 188
           LL+W F TYL NS+          H E+ W+++L +AA  LG++GNICL FLF PVARGS
Sbjct: 148 LLLWCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVARGS 207

Query: 189 SLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVA 248
           SLLP +GLTSESSIKYHIWLGHM MA F+ HG+C+++YWA+ H +SQM+ W    VSN+A
Sbjct: 208 SLLPAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMIMWDTKGVSNLA 267

Query: 249 GEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLF 308
           GE++L  GL++WATT+P +RR+FFE+F YTH+LYI+F++FF+ HVGIS++ I LPGFY+F
Sbjct: 268 GEIALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALPGFYIF 327

Query: 309 LLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHP 368
           L+DRFLRFLQSR  VRLL+AR+LP  T+EL FSK+  L Y+PTS +F+NIP ISKLQWHP
Sbjct: 328 LVDRFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISKLQWHP 387

Query: 369 FTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQF 428
           FT+TS + LEPE LS+VIK EG WSTKL+Q LSS   ID  L VS+EGPYGP S  FL+ 
Sbjct: 388 FTITSSSKLEPEKLSIVIKKEGKWSTKLHQRLSSSDQID-RLAVSVEGPYGPASADFLRH 447

Query: 429 DSLIMISGGSGITPFISIIRHLIHNSNSHQ-QIPKLLLISAFKNSSDLTFLHLLLPLSGA 488
           ++L+M+ GGSGITPFIS+IR LI  S     +IPK+ LI AFK SS+++ L L+LPLSG 
Sbjct: 448 EALVMVCGGSGITPFISVIRDLIATSQKETCKIPKITLICAFKKSSEISMLDLVLPLSGL 507

Query: 489 NTL----ENFQIEAYVTRDKSPKDENP--QFRTVIFKPHEGDSPATAILGPNGWLWLAGI 548
            T      N +IEA++TRD    DE    + +T+ FKP   D   ++ILGPN WLWL  I
Sbjct: 508 ETELSSDINIKIEAFITRDNDAGDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWLGAI 567

Query: 549 ICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNK 608
           + SSF IF+ ++GI+TRYYIYPID N+N +++L+ ++ +++L + + I+   +AA++WNK
Sbjct: 568 LASSFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVISVSIMATCSAAMLWNK 627

Query: 609 RKLAK-EGRQIQNVEGATPNASP-SAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLP 668
           +K  K E +Q+QNV+  +P +SP S+ G++++   E+ES P E+L Q  ++++GERPNL 
Sbjct: 628 KKYGKVESKQVQNVDRPSPTSSPTSSWGYNSL--REIESTPQESLVQRTNLHFGERPNLK 687

Query: 669 RILLERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ++LL+ +G +VGVL  GPKKMRQ+VA IC SGLA NLHF+SISF+W
Sbjct: 688 KLLLDVEGSSVGVLVCGPKKMRQKVAEICSSGLAENLHFESISFSW 725

BLAST of CmaCh04G012500 vs. ExPASy Swiss-Prot
Match: Q9LMM2 (Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 3.4e-201
Identity = 364/715 (50.91%), Postives = 505/715 (70.63%), Query Frame = 0

Query: 3   VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFP 62
           V+   I+ L+ ++ +G + IW MMPT+TY+  WL  ++ K   S Y+G  G +LL+Y FP
Sbjct: 10  VIDKVIKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFP 69

Query: 63  ILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMW---KRPGFVKGPLGIVSWTELAL 122
           ++ +A LGC+Y+H++   +   V   N G +   A +   +RP  V GPLGIV+ TE+  
Sbjct: 70  MILLAFLGCIYLHLK---KSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMF 129

Query: 123 LAMFVALLVWSFATYLNNSY----------HSENEWESRLGTAAYWLGVVGNICLVFLFF 182
           L MF+ALL+WS A Y+  ++             N W++RL   A  +G+VGNICL FLF+
Sbjct: 130 LTMFMALLLWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFY 189

Query: 183 PVARGSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNL-SQMLEWAK 242
           PVARGSSLL  +GLTSESSIKYHIWLGH+ M  F++HG+C+ +YW + + L S+MLEW +
Sbjct: 190 PVARGSSLLAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQLVSKMLEWDR 249

Query: 243 TDVSNVAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIM 302
           T VSN+AGE++LV GL++W TT+P +RR+ FE+F Y+H+LYI+F++FF+FHVGIS+A I 
Sbjct: 250 TAVSNLAGEIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHALIP 309

Query: 303 LPGFYLFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCI 362
           LPGFY+FL+DRFLRFLQSR  V+L+SAR+LPC T+ELNFSK+P L Y+PTST+F+NIP I
Sbjct: 310 LPGFYIFLVDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIPSI 369

Query: 363 SKLQWHPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAID-NHLQVSLEGPYGP 422
           SKLQWHPFT+ S + LEPETLSV+IK +G WSTKLY  LSS S+   N L VS+EGPYGP
Sbjct: 370 SKLQWHPFTIISSSKLEPETLSVMIKSQGKWSTKLYDMLSSSSSDQINRLAVSVEGPYGP 429

Query: 423 VSTSFLQFDSLIMISGGSGITPFISIIRHLIHNSNSHQ-QIPKLLLISAFKNSSDLTFLH 482
            ST FL+ +SL+M+SGGSGITPFISI+R L + S++H+ +IPK+ LI AFKNSSDL+ L 
Sbjct: 430 SSTDFLRHESLVMVSGGSGITPFISIVRDLFYMSSTHKCKIPKMTLICAFKNSSDLSMLD 489

Query: 483 LLLPLSGANT----LENFQIEAYVTR-DKSPKDENPQFRTVI----FKPHEGDSPATAIL 542
           L+LP SG  T      + QI+A+VTR +K+   E+   R +I    FKP+  D P + IL
Sbjct: 490 LILPTSGLTTDMASFVDIQIKAFVTREEKTSVKESTHNRNIIKTRHFKPNVSDQPISPIL 549

Query: 543 GPNGWLWLAGIICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIV 602
           GPN WL LA I+ SSF IF+ ++ I+TRY+I+PID NS + +  + +S ++++ + + +V
Sbjct: 550 GPNSWLCLAAILSSSFMIFIVIIAIITRYHIHPIDQNS-EKYTWAYKSLIYLVSISITVV 609

Query: 603 MAATAAVIWNKRKLAKEGRQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHV 662
             +TAA++WNK+K   +  Q                  DN+  + +ES P + ++QS  +
Sbjct: 610 TTSTAAMLWNKKKYYAKNDQY----------------VDNLSPVIIESSPQQLISQSTDI 669

Query: 663 YYGERPNLPRILLERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           +YGERPNL ++L+  KG +VG+L  GPKKMRQ+VA IC  G A NLHF+SISF+W
Sbjct: 670 HYGERPNLNKLLVGLKGSSVGILVCGPKKMRQKVAKICSFGSAENLHFESISFSW 704

BLAST of CmaCh04G012500 vs. ExPASy Swiss-Prot
Match: F4I4K7 (Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO3 PE=2 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 5.1e-197
Identity = 352/703 (50.07%), Postives = 497/703 (70.70%), Query Frame = 0

Query: 3   VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFP 62
           V+   I++L  ++ +G + IW MMPT+TY+  WL  ++ K   S YFG  G +LL+Y FP
Sbjct: 33  VIKNVIKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFP 92

Query: 63  ILFIAVLGCVYVHIERRCREQNVKIR-NRGNDNGLAMWKRPGFVKGPLGIVSWTELALLA 122
           ++ +A LG +Y+H++++ R      R +R   +     KRP  VK  LGIV+ TE+  L 
Sbjct: 93  MILLASLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLM 152

Query: 123 MFVALLVWSFATYLNNSY----------HSENEWESRLGTAAYWLGVVGNICLVFLFFPV 182
           MF+ALL+WS A Y  +++            +N W++RL + A  LG+ GNICL FLF+PV
Sbjct: 153 MFMALLLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNICLGFLFYPV 212

Query: 183 ARGSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDV 242
           ARGSSLL  +GLTSESS KYHIWLG++ M  F++HG+C+ +YW +T+ +SQMLEW +T +
Sbjct: 213 ARGSSLLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQMLEWDRTGI 272

Query: 243 SNVAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPG 302
           S++AGE++LV GL++WATTFP +RR+FFE+F YTH+LY++F++FF+FHVGISYA I  PG
Sbjct: 273 SHLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISYALISFPG 332

Query: 303 FYLFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKL 362
           FY+F++DRFLRFLQSR  V+L+SAR+LPC T+ELNFSK+P L Y+PTS LF+NIP ISKL
Sbjct: 333 FYIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVNIPSISKL 392

Query: 363 QWHPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTS 422
           QWHPFT+TS + LEP+ LSV+IK +G WS+KL+  L+S + ID HL VS+EGPYGP ST 
Sbjct: 393 QWHPFTITSSSKLEPKKLSVMIKSQGKWSSKLHHMLASSNQID-HLAVSVEGPYGPASTD 452

Query: 423 FLQFDSLIMISGGSGITPFISIIRHLIH-NSNSHQQIPKLLLISAFKNSSDLTFLHLLLP 482
           +L+ DSL+M+SGGSGITPFISIIR L++ +S +  + PK+ LI AFKNSSDL+ L+L+LP
Sbjct: 453 YLRHDSLVMVSGGSGITPFISIIRDLLYVSSTNAYKTPKITLICAFKNSSDLSMLNLILP 512

Query: 483 LS-GANTLENFQIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGII 542
            S   ++  + QI+A+VTR+K         +T+ FKP+  D P + ILGPN WLWLA I+
Sbjct: 513 NSTEISSFIDIQIKAFVTREKVSTCNMNIIKTLSFKPYVSDQPISPILGPNSWLWLATIL 572

Query: 543 CSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKR 602
            SSF IF+ ++ I++RY+IYPID +S + +  +  S +++L + + +V  +T A++ NK+
Sbjct: 573 SSSFMIFIIIIAIISRYHIYPIDQSSKE-YTSAYTSLIYLLAISISVVATSTVAMLCNKK 632

Query: 603 KLAKEGRQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRIL 662
              K     QNV+  +P              + +ES P + L +  +++YGERPNL ++L
Sbjct: 633 SYFK--GLYQNVDALSP--------------LMIESSPDQLLPEFTNIHYGERPNLNKLL 692

Query: 663 LERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           +  KG +VGVL  GP+KMR+EVA IC  G A NL F+SISF W
Sbjct: 693 VGLKGSSVGVLVCGPRKMREEVAKICSFGSAANLQFESISFNW 717

BLAST of CmaCh04G012500 vs. ExPASy Swiss-Prot
Match: Q8W110 (Ferric reduction oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=FRO4 PE=2 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 2.2e-184
Identity = 346/712 (48.60%), Postives = 483/712 (67.84%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           MG +   ++ L+ +LF+GW+ +W M+ TN ++ +W PK+  K  +++YFG +GT+L++ T
Sbjct: 1   MGNMRSLVKTLMVVLFLGWILVWIMISTNLFKSKWTPKL-SKYLNTTYFGPQGTNLVLLT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
            P++FIAVL CVY+HI+++  +   + + +       +  R   V  PLGIV+ TEL   
Sbjct: 61  VPMMFIAVLSCVYLHIQKKPTQPQREWKLK------RIMGRVIMVMNPLGIVTATELTFS 120

Query: 121 AMFVALLVWSFATYLNNSYH-------SENEWESRLGTAAYWLGVVGNICLVFLFFPVAR 180
            +FVALL WS   YL  SYH       +   W+++       +G VGNIC  FLFFPV R
Sbjct: 121 LLFVALLAWSLYNYLYLSYHVHLHNDDNAKIWQAKFRAFGLRIGYVGNICWAFLFFPVTR 180

Query: 181 GSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSN 240
            S++LPL+GLTSESSIKYHIWLGH++   F  H + F++YWA  + L +   W  T V N
Sbjct: 181 ASTILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLMETFAWNPTYVPN 240

Query: 241 VAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFY 300
           +AG +++V G+ +W T+ P  RRK FE+F YTHHLY L+IVF++ HVG S+ C++LP  +
Sbjct: 241 LAGTIAMVIGIAMWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYVIHVGDSWFCMILPNIF 300

Query: 301 LFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQW 360
           LF +DR+LRFLQS +R RL+SAR+LP   LEL FSK PGL YTPTS LFL++P ISK+QW
Sbjct: 301 LFFIDRYLRFLQSTKRSRLVSARILPSDNLELTFSKTPGLHYTPTSILFLHVPSISKIQW 360

Query: 361 HPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFL 420
           HPFT+TS ++LE +TLSVVI+ +G+W+ KLY  LS  S+ID+ L+VS EGPYGP S    
Sbjct: 361 HPFTITSSSNLEKDTLSVVIRRQGSWTQKLYTHLS--SSIDS-LEVSTEGPYGPNSFDVS 420

Query: 421 QFDSLIMISGGSGITPFISIIRHLIHNS-NSHQQIPKLLLISAFKNSSDLTFLHLLLPLS 480
           + +SLI++SGGSGITPFIS+IR LI  S N   ++P +LL+ +FK+  DL FL L+ PL 
Sbjct: 421 RHNSLILVSGGSGITPFISVIRELISQSQNKSTKLPDVLLVCSFKHYHDLAFLDLIFPLD 480

Query: 481 -GANTLE--NFQIEAYVTR-DKSPK--DENPQFRTVIFKPHEGDSPATAILGPNGWLWLA 540
             A+ +   N +IEAY+TR DK P+  D++   +T  FKP   DSP + +LGPN +LWL 
Sbjct: 481 MSASDISRLNLRIEAYITREDKKPETTDDHRLLQTKWFKPQPLDSPISPVLGPNNFLWLG 540

Query: 541 GIICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIW 600
            +I SSF +FL L+GIVTRYYIYP+D N+  ++N S R    M     CI ++++   +W
Sbjct: 541 VVILSSFVMFLLLIGIVTRYYIYPVDHNTGSIYNFSYRGLWDMFLGSACIFISSSVVFLW 600

Query: 601 NKRKLAKEG-----RQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGE 660
            K K  KEG      Q+Q+VE  TP +SP +    +  E ELESVP++++ Q+  V++G 
Sbjct: 601 RK-KQNKEGDKEFKNQVQSVEFQTPTSSPGSWFHGH--ERELESVPYQSIVQATSVHFGS 660

Query: 661 RPNLPRILLERKG-ENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           +PNL +ILLE +G E+VGV+  GP+KMR EVA IC SGLA NLHF++ISF W
Sbjct: 661 KPNLKKILLEAEGSEDVGVMVCGPRKMRHEVAKICSSGLAKNLHFEAISFNW 699

BLAST of CmaCh04G012500 vs. ExPASy Swiss-Prot
Match: Q9FLW2 (Ferric reduction oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=FRO5 PE=2 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 1.2e-182
Identity = 348/725 (48.00%), Postives = 486/725 (67.03%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           MG +   +++L+ +LF+GW+F+W M+ TN +++ W PK+  K   ++YFG +G +L++ T
Sbjct: 1   MGNMRSLVKMLMVVLFLGWIFVWIMISTNRFQNIWTPKLA-KYLKTTYFGPQGMNLVLLT 60

Query: 61  FPILFIAVLGCVYVHIER---------RCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGI 120
            P++FIAVL CVY+H ++         +CRE  VK R            R   V  PLGI
Sbjct: 61  VPMMFIAVLSCVYLHTQKQPSQTQSLYKCREWKVKGR----------MGRVMMVMNPLGI 120

Query: 121 VSWTELALLAMFVALLVWSFATYLNNSYH-------SENE---WESRLGTAAYWLGVVGN 180
           V+ TEL    +F+ALLVW+ + YL  SYH       + N+   W+++       +G VG+
Sbjct: 121 VTATELTFSLLFLALLVWALSNYLYLSYHVHLHNHDNANDMCRWQAKFRAFGLRIGYVGH 180

Query: 181 ICLVFLFFPVARGSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLS 240
            C  FLFFPV R S++LPL+GLTSESSIKYHIWLGH++   F  H + F++YWA  + L 
Sbjct: 181 YCWAFLFFPVTRASTILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMVNKLM 240

Query: 241 QMLEWAKTDVSNVAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVG 300
           +   W  T V N+AG +++V G+ IW T+ P  RRK FE+F YTHHLY L+IVF+  HVG
Sbjct: 241 ETFAWNATYVPNLAGTIAMVIGIAIWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYAIHVG 300

Query: 301 ISYACIMLPGFYLFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTL 360
            S+ C++LP  +LF +DR+LRFLQS +R RL+SA++LP   LEL F+K  GL YTPTS L
Sbjct: 301 DSWFCMILPNIFLFFIDRYLRFLQSTKRSRLVSAKILPSDNLELTFAKTSGLHYTPTSIL 360

Query: 361 FLNIPCISKLQWHPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSL 420
           FL++P ISKLQWHPFT+TS ++LE +TLSVVI+ +G+W+ KLY  LS  S+ID+ L+VS 
Sbjct: 361 FLHVPSISKLQWHPFTITSSSNLEKDTLSVVIRKQGSWTQKLYTHLS--SSIDS-LEVST 420

Query: 421 EGPYGPVSTSFLQFDSLIMISGGSGITPFISIIRHLIHNS-NSHQQIPKLLLISAFKNSS 480
           EGPYGP S    + DSLI++ GGSG+TPFIS+IR LI  S N   ++P +LL+ AFKN  
Sbjct: 421 EGPYGPNSFDVSRHDSLILVGGGSGVTPFISVIRELIFQSQNRSTKLPNVLLVCAFKNYH 480

Query: 481 DLTFLHLLLP----LSGANTLENFQIEAYVTR-DKSPK--DENPQFRTVIFKPHEGDSPA 540
           DL FL L+ P    +S  + L N +IEAY+TR DK P+  D++   +T  FKP   DSP 
Sbjct: 481 DLAFLDLIFPSDISVSDISKL-NLRIEAYITREDKKPETTDDHKLLQTKWFKPQPLDSPI 540

Query: 541 TAILGPNGWLWLAGIICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLC 600
           + +LGPN +LWL  +I SSF +FL L+GIVTRYYIYP+D N+  ++N + R    M   C
Sbjct: 541 SPVLGPNNFLWLGVVILSSFVMFLLLIGIVTRYYIYPVDHNTGSIYNFTYRVLWVMFLGC 600

Query: 601 LCIVMAATAAVIWNKRKLAKEG-----RQIQNVEGATPNASPSAMGFDNVGEMELESVPF 660
           +CI ++++   +W K++  KEG     +Q+Q+VE  TP +SP +    +  E ELESVP+
Sbjct: 601 VCIFISSSIIFLWRKKE-NKEGDKDSKKQVQSVEFQTPTSSPGSWFHGH--ERELESVPY 660

Query: 661 EALAQSVHVYYGERPNLPRILLERKG-ENVGVLASGPKKMRQEVAAICGSGLAPNLHFQS 693
           +++ Q+  V++G +PNL +IL E +G E+VGV+  GPKKMR EVA IC SGLA NLHF++
Sbjct: 661 QSIVQATSVHFGSKPNLKKILFEAEGSEDVGVMVCGPKKMRHEVAKICSSGLAKNLHFEA 707

BLAST of CmaCh04G012500 vs. ExPASy TrEMBL
Match: A0A6J1KFY3 (ferric reduction oxidase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493542 PE=4 SV=1)

HSP 1 Score: 1393.6 bits (3606), Expect = 0.0e+00
Identity = 692/692 (100.00%), Postives = 692/692 (100.00%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT
Sbjct: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 692

BLAST of CmaCh04G012500 vs. ExPASy TrEMBL
Match: A0A6J1K9Q4 (ferric reduction oxidase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493542 PE=4 SV=1)

HSP 1 Score: 1384.8 bits (3583), Expect = 0.0e+00
Identity = 690/692 (99.71%), Postives = 690/692 (99.71%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT
Sbjct: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRCREQNVKI  RGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCREQNVKI--RGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 690

BLAST of CmaCh04G012500 vs. ExPASy TrEMBL
Match: A0A6J1GZA5 (ferric reduction oxidase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458241 PE=4 SV=1)

HSP 1 Score: 1374.4 bits (3556), Expect = 0.0e+00
Identity = 681/692 (98.41%), Postives = 688/692 (99.42%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           M VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSR SSYFGSEGTSLLMYT
Sbjct: 1   MRVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRHSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRC+EQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCKEQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYH+ENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHNENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHG+CFVVYWAATH+LSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGVCFVVYWAATHDLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSF QFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFPQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLP+SGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPVSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKP+EGDSPATAILGPNGWLWLAG+ICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPYEGDSPATAILGPNGWLWLAGVICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSV VYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVRVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 692

BLAST of CmaCh04G012500 vs. ExPASy TrEMBL
Match: A0A6J1GY09 (ferric reduction oxidase 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458241 PE=4 SV=1)

HSP 1 Score: 1365.5 bits (3533), Expect = 0.0e+00
Identity = 679/692 (98.12%), Postives = 686/692 (99.13%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           M VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSR SSYFGSEGTSLLMYT
Sbjct: 1   MRVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRHSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRC+EQNVKI  RGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCKEQNVKI--RGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYH+ENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHNENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHG+CFVVYWAATH+LSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGVCFVVYWAATHDLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSF QFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFPQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLP+SGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPVSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKP+EGDSPATAILGPNGWLWLAG+ICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPYEGDSPATAILGPNGWLWLAGVICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSV VYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVRVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 690

BLAST of CmaCh04G012500 vs. ExPASy TrEMBL
Match: A0A1S3BD76 (ferric reduction oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103488760 PE=4 SV=1)

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 532/693 (76.77%), Postives = 610/693 (88.02%), Query Frame = 0

Query: 3   VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFP 62
           VLSLAIRVLV ILF+GW+F+W MMPTNTYR +WLPKI++K+  S+YFGSEGTSLLMYTFP
Sbjct: 6   VLSLAIRVLVLILFLGWIFLWVMMPTNTYRKKWLPKIREKTYQSTYFGSEGTSLLMYTFP 65

Query: 63  ILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALLAM 122
           ILFIA+LGCVY H+E++  +QN++ R     +GLAMWK+P  VKGPLGIVSWTEL LL M
Sbjct: 66  ILFIAILGCVYNHLEKKRSDQNIETRKGTKHHGLAMWKQPAIVKGPLGIVSWTELTLLTM 125

Query: 123 FVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPLLG 182
           F+ LLVWSFATYL+NS+ SE  WE RLG+A +WLG+VGNICLVFLFFPVARGSSLLPLLG
Sbjct: 126 FITLLVWSFATYLHNSFISEKMWEIRLGSAGFWLGIVGNICLVFLFFPVARGSSLLPLLG 185

Query: 183 LTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSLVC 242
           LTSE  IKYHIWLGHMTMAFFSAHG+CFV+YW AT+N+SQML+WAKTDVSN+AGE++LV 
Sbjct: 186 LTSEGCIKYHIWLGHMTMAFFSAHGVCFVIYWVATNNISQMLKWAKTDVSNIAGELALVF 245

Query: 243 GLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRFLR 302
           GLI+WATT P VRRKFFELFLYTH+LYILFIVFFIFHVGISYAC+MLPGFYLF++DR+LR
Sbjct: 246 GLIMWATTIPRVRRKFFELFLYTHYLYILFIVFFIFHVGISYACVMLPGFYLFVIDRYLR 305

Query: 303 FLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTSHA 362
           FLQSRRRVRL SARLLPC TLELNFSKHPGLKY PTST+F+NIP ISKLQWHPFT+TSH+
Sbjct: 306 FLQSRRRVRLQSARLLPCQTLELNFSKHPGLKYNPTSTMFINIPSISKLQWHPFTITSHS 365

Query: 363 DLEPETLSVVIKCEGTWSTKLYQTL--SSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 422
           DLEPE LSVVIKCEGTWS+KLY+TL  SS SAI++HLQVSLEGPYGPVSTSFLQFD+L+M
Sbjct: 366 DLEPEKLSVVIKCEGTWSSKLYKTLSSSSSSAINDHLQVSLEGPYGPVSTSFLQFDTLLM 425

Query: 423 ISGGSGITPFISIIRHLIHNSNS-HQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLEN 482
           ISGGSGITPFISII+H+IHNSNS H + PKLLLISAFK ++DLTFLHL   L    T  N
Sbjct: 426 ISGGSGITPFISIIKHIIHNSNSHHNKTPKLLLISAFKTTADLTFLHL---LQTPTTPHN 485

Query: 483 FQIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLAL 542
           FQIEAYVTR+KSP++ENPQ R+VIFK H G+S A AILG NGW+WLAG+ICSSFGIFL L
Sbjct: 486 FQIEAYVTREKSPQNENPQIRSVIFKSHIGESAAAAILGRNGWVWLAGVICSSFGIFLIL 545

Query: 543 MGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQ 602
           +G++ RYYIYPIDGN+NDVF+L L+SFLHMLGLC  +   ATAAVIWNKR+ AKEG+QIQ
Sbjct: 546 IGVLNRYYIYPIDGNTNDVFDLGLKSFLHMLGLCFGVFTVATAAVIWNKRETAKEGKQIQ 605

Query: 603 NVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGV 662
           NVEG TPN SP A+G +   E+ELES P E + QSV+V+YGERP+LPRI+ E KGEN+GV
Sbjct: 606 NVEGGTPNGSPCAIGCER--EIELESNPLEVIDQSVNVHYGERPDLPRIIGECKGENIGV 665

Query: 663 LASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           LASGPKK+RQEVAAICGS  A NLHF SISFTW
Sbjct: 666 LASGPKKLRQEVAAICGSASAKNLHFHSISFTW 693

BLAST of CmaCh04G012500 vs. NCBI nr
Match: XP_022999034.1 (ferric reduction oxidase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1393.6 bits (3606), Expect = 0.0e+00
Identity = 692/692 (100.00%), Postives = 692/692 (100.00%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT
Sbjct: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 692

BLAST of CmaCh04G012500 vs. NCBI nr
Match: XP_022999042.1 (ferric reduction oxidase 2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1384.8 bits (3583), Expect = 0.0e+00
Identity = 690/692 (99.71%), Postives = 690/692 (99.71%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT
Sbjct: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRCREQNVKI  RGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCREQNVKI--RGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 690

BLAST of CmaCh04G012500 vs. NCBI nr
Match: XP_022956524.1 (ferric reduction oxidase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1374.4 bits (3556), Expect = 0.0e+00
Identity = 681/692 (98.41%), Postives = 688/692 (99.42%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           M VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSR SSYFGSEGTSLLMYT
Sbjct: 1   MRVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRHSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRC+EQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCKEQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYH+ENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHNENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHG+CFVVYWAATH+LSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGVCFVVYWAATHDLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSF QFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFPQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLP+SGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPVSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKP+EGDSPATAILGPNGWLWLAG+ICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPYEGDSPATAILGPNGWLWLAGVICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSV VYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVRVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 692

BLAST of CmaCh04G012500 vs. NCBI nr
Match: XP_022956525.1 (ferric reduction oxidase 2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1365.5 bits (3533), Expect = 0.0e+00
Identity = 679/692 (98.12%), Postives = 686/692 (99.13%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           M VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSR SSYFGSEGTSLLMYT
Sbjct: 1   MRVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRHSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRC+EQNVKI  RGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCKEQNVKI--RGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYH+ENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHNENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHG+CFVVYWAATH+LSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGVCFVVYWAATHDLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSF QFDSLIM
Sbjct: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFPQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLP+SGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPVSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKP+EGDSPATAILGPNGWLWLAG+ICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPYEGDSPATAILGPNGWLWLAGVICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGEMELESVPFEALAQSV VYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVRVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 690

BLAST of CmaCh04G012500 vs. NCBI nr
Match: KAG6601053.1 (Ferric reduction oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1359.4 bits (3517), Expect = 0.0e+00
Identity = 678/692 (97.98%), Postives = 683/692 (98.70%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           M VLSLAIRVLVFILFVGWLFIWF+MPTNTYRHQWLPKIQDKSR SSYFGSEGTSLLMYT
Sbjct: 1   MRVLSLAIRVLVFILFVGWLFIWFVMPTNTYRHQWLPKIQDKSRHSSYFGSEGTSLLMYT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
           FPILFIAVLGCVYVHIERRC+EQNVKI  RGNDNGLAMWKRPGFVKGPLGIVSWTELALL
Sbjct: 61  FPILFIAVLGCVYVHIERRCKEQNVKI--RGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120

Query: 121 AMFVALLVWSFATYLNNSYHSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180
           AMFVALLVWSFATYLNNSYH+ENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL
Sbjct: 121 AMFVALLVWSFATYLNNSYHNENEWESRLGTAAYWLGVVGNICLVFLFFPVARGSSLLPL 180

Query: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240
           LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL
Sbjct: 181 LGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVAGEVSL 240

Query: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300
           VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF
Sbjct: 241 VCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLFLLDRF 300

Query: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360
           LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS
Sbjct: 301 LRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHPFTVTS 360

Query: 361 HADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420
           HADLEP+TLSV IKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM
Sbjct: 361 HADLEPDTLSVAIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQFDSLIM 420

Query: 421 ISGGSGITPFISIIRHLIHNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480
           ISGGSGITPFISIIRHLI+NSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF
Sbjct: 421 ISGGSGITPFISIIRHLIYNSNSHQQIPKLLLISAFKNSSDLTFLHLLLPLSGANTLENF 480

Query: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIICSSFGIFLALM 540
           QIEAYVTRDKSPKDENPQFRTVIFKPHE DSPATAILGPNGWLWLA IICSSFGIFLALM
Sbjct: 481 QIEAYVTRDKSPKDENPQFRTVIFKPHEEDSPATAILGPNGWLWLAWIICSSFGIFLALM 540

Query: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600
           GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN
Sbjct: 541 GIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRKLAKEGRQIQN 600

Query: 601 VEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILLERKGENVGVL 660
           VEGATPNASPSAMGFDNVGE ELESVPFEALAQSV VYYGERPNLPRILLERKGENVGVL
Sbjct: 601 VEGATPNASPSAMGFDNVGETELESVPFEALAQSVRVYYGERPNLPRILLERKGENVGVL 660

Query: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW
Sbjct: 661 ASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 690

BLAST of CmaCh04G012500 vs. TAIR 10
Match: AT1G01580.1 (ferric reduction oxidase 2 )

HSP 1 Score: 763.8 bits (1971), Expect = 1.2e-220
Identity = 382/706 (54.11%), Postives = 522/706 (73.94%), Query Frame = 0

Query: 9   RVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFPILFIAV 68
           + L+ ++F+G + +W MMPT TYR +WLP ++ K  +S+YFG+ GT+L MY FP++ +A 
Sbjct: 28  KFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFPMMVVAC 87

Query: 69  LGCVYVHIERRCREQNVKIRNRGNDNGLAMW---KRPGFVKGPLGIVSWTELALLAMFVA 128
           LGCVY+H + R    ++    +G      +W   ++P  VKGPLGIVS TE+  LAMFVA
Sbjct: 88  LGCVYLHFKNRKSPHHIDRETKG-----GVWSKLRKPMLVKGPLGIVSVTEITFLAMFVA 147

Query: 129 LLVWSFATYLNNSY----------HSENEWESRLGTAAYWLGVVGNICLVFLFFPVARGS 188
           LL+W F TYL NS+          H E+ W+++L +AA  LG++GNICL FLF PVARGS
Sbjct: 148 LLLWCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVARGS 207

Query: 189 SLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSNVA 248
           SLLP +GLTSESSIKYHIWLGHM MA F+ HG+C+++YWA+ H +SQM+ W    VSN+A
Sbjct: 208 SLLPAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMIMWDTKGVSNLA 267

Query: 249 GEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFYLF 308
           GE++L  GL++WATT+P +RR+FFE+F YTH+LYI+F++FF+ HVGIS++ I LPGFY+F
Sbjct: 268 GEIALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALPGFYIF 327

Query: 309 LLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQWHP 368
           L+DRFLRFLQSR  VRLL+AR+LP  T+EL FSK+  L Y+PTS +F+NIP ISKLQWHP
Sbjct: 328 LVDRFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISKLQWHP 387

Query: 369 FTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFLQF 428
           FT+TS + LEPE LS+VIK EG WSTKL+Q LSS   ID  L VS+EGPYGP S  FL+ 
Sbjct: 388 FTITSSSKLEPEKLSIVIKKEGKWSTKLHQRLSSSDQID-RLAVSVEGPYGPASADFLRH 447

Query: 429 DSLIMISGGSGITPFISIIRHLIHNSNSHQ-QIPKLLLISAFKNSSDLTFLHLLLPLSGA 488
           ++L+M+ GGSGITPFIS+IR LI  S     +IPK+ LI AFK SS+++ L L+LPLSG 
Sbjct: 448 EALVMVCGGSGITPFISVIRDLIATSQKETCKIPKITLICAFKKSSEISMLDLVLPLSGL 507

Query: 489 NTL----ENFQIEAYVTRDKSPKDENP--QFRTVIFKPHEGDSPATAILGPNGWLWLAGI 548
            T      N +IEA++TRD    DE    + +T+ FKP   D   ++ILGPN WLWL  I
Sbjct: 508 ETELSSDINIKIEAFITRDNDAGDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWLGAI 567

Query: 549 ICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNK 608
           + SSF IF+ ++GI+TRYYIYPID N+N +++L+ ++ +++L + + I+   +AA++WNK
Sbjct: 568 LASSFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVISVSIMATCSAAMLWNK 627

Query: 609 RKLAK-EGRQIQNVEGATPNASP-SAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLP 668
           +K  K E +Q+QNV+  +P +SP S+ G++++   E+ES P E+L Q  ++++GERPNL 
Sbjct: 628 KKYGKVESKQVQNVDRPSPTSSPTSSWGYNSL--REIESTPQESLVQRTNLHFGERPNLK 687

Query: 669 RILLERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           ++LL+ +G +VGVL  GPKKMRQ+VA IC SGLA NLHF+SISF+W
Sbjct: 688 KLLLDVEGSSVGVLVCGPKKMRQKVAEICSSGLAENLHFESISFSW 725

BLAST of CmaCh04G012500 vs. TAIR 10
Match: AT1G01590.1 (ferric reduction oxidase 1 )

HSP 1 Score: 703.0 bits (1813), Expect = 2.4e-202
Identity = 364/715 (50.91%), Postives = 505/715 (70.63%), Query Frame = 0

Query: 3   VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFP 62
           V+   I+ L+ ++ +G + IW MMPT+TY+  WL  ++ K   S Y+G  G +LL+Y FP
Sbjct: 10  VIDKVIKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFP 69

Query: 63  ILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMW---KRPGFVKGPLGIVSWTELAL 122
           ++ +A LGC+Y+H++   +   V   N G +   A +   +RP  V GPLGIV+ TE+  
Sbjct: 70  MILLAFLGCIYLHLK---KSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMF 129

Query: 123 LAMFVALLVWSFATYLNNSY----------HSENEWESRLGTAAYWLGVVGNICLVFLFF 182
           L MF+ALL+WS A Y+  ++             N W++RL   A  +G+VGNICL FLF+
Sbjct: 130 LTMFMALLLWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFY 189

Query: 183 PVARGSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNL-SQMLEWAK 242
           PVARGSSLL  +GLTSESSIKYHIWLGH+ M  F++HG+C+ +YW + + L S+MLEW +
Sbjct: 190 PVARGSSLLAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQLVSKMLEWDR 249

Query: 243 TDVSNVAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIM 302
           T VSN+AGE++LV GL++W TT+P +RR+ FE+F Y+H+LYI+F++FF+FHVGIS+A I 
Sbjct: 250 TAVSNLAGEIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHALIP 309

Query: 303 LPGFYLFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCI 362
           LPGFY+FL+DRFLRFLQSR  V+L+SAR+LPC T+ELNFSK+P L Y+PTST+F+NIP I
Sbjct: 310 LPGFYIFLVDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIPSI 369

Query: 363 SKLQWHPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAID-NHLQVSLEGPYGP 422
           SKLQWHPFT+ S + LEPETLSV+IK +G WSTKLY  LSS S+   N L VS+EGPYGP
Sbjct: 370 SKLQWHPFTIISSSKLEPETLSVMIKSQGKWSTKLYDMLSSSSSDQINRLAVSVEGPYGP 429

Query: 423 VSTSFLQFDSLIMISGGSGITPFISIIRHLIHNSNSHQ-QIPKLLLISAFKNSSDLTFLH 482
            ST FL+ +SL+M+SGGSGITPFISI+R L + S++H+ +IPK+ LI AFKNSSDL+ L 
Sbjct: 430 SSTDFLRHESLVMVSGGSGITPFISIVRDLFYMSSTHKCKIPKMTLICAFKNSSDLSMLD 489

Query: 483 LLLPLSGANT----LENFQIEAYVTR-DKSPKDENPQFRTVI----FKPHEGDSPATAIL 542
           L+LP SG  T      + QI+A+VTR +K+   E+   R +I    FKP+  D P + IL
Sbjct: 490 LILPTSGLTTDMASFVDIQIKAFVTREEKTSVKESTHNRNIIKTRHFKPNVSDQPISPIL 549

Query: 543 GPNGWLWLAGIICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIV 602
           GPN WL LA I+ SSF IF+ ++ I+TRY+I+PID NS + +  + +S ++++ + + +V
Sbjct: 550 GPNSWLCLAAILSSSFMIFIVIIAIITRYHIHPIDQNS-EKYTWAYKSLIYLVSISITVV 609

Query: 603 MAATAAVIWNKRKLAKEGRQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHV 662
             +TAA++WNK+K   +  Q                  DN+  + +ES P + ++QS  +
Sbjct: 610 TTSTAAMLWNKKKYYAKNDQY----------------VDNLSPVIIESSPQQLISQSTDI 669

Query: 663 YYGERPNLPRILLERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           +YGERPNL ++L+  KG +VG+L  GPKKMRQ+VA IC  G A NLHF+SISF+W
Sbjct: 670 HYGERPNLNKLLVGLKGSSVGILVCGPKKMRQKVAKICSFGSAENLHFESISFSW 704

BLAST of CmaCh04G012500 vs. TAIR 10
Match: AT1G23020.1 (ferric reduction oxidase 3 )

HSP 1 Score: 691.0 bits (1782), Expect = 9.5e-199
Identity = 351/702 (50.00%), Postives = 496/702 (70.66%), Query Frame = 0

Query: 3   VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFP 62
           V+   I++L  ++ +G + IW MMPT+TY+  WL  ++ K   S YFG  G +LL+Y FP
Sbjct: 33  VIKNVIKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFP 92

Query: 63  ILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALLAM 122
           ++ +A LG +Y+H++++ R       +R   +     KRP  VK  LGIV+ TE+  L M
Sbjct: 93  MILLASLGSIYLHLKKQTRVNQFNRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMM 152

Query: 123 FVALLVWSFATYLNNSY----------HSENEWESRLGTAAYWLGVVGNICLVFLFFPVA 182
           F+ALL+WS A Y  +++            +N W++RL + A  LG+ GNICL FLF+PVA
Sbjct: 153 FMALLLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNICLGFLFYPVA 212

Query: 183 RGSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVS 242
           RGSSLL  +GLTSESS KYHIWLG++ M  F++HG+C+ +YW +T+ +SQMLEW +T +S
Sbjct: 213 RGSSLLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQMLEWDRTGIS 272

Query: 243 NVAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGF 302
           ++AGE++LV GL++WATTFP +RR+FFE+F YTH+LY++F++FF+FHVGISYA I  PGF
Sbjct: 273 HLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISYALISFPGF 332

Query: 303 YLFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQ 362
           Y+F++DRFLRFLQSR  V+L+SAR+LPC T+ELNFSK+P L Y+PTS LF+NIP ISKLQ
Sbjct: 333 YIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVNIPSISKLQ 392

Query: 363 WHPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSF 422
           WHPFT+TS + LEP+ LSV+IK +G WS+KL+  L+S + ID HL VS+EGPYGP ST +
Sbjct: 393 WHPFTITSSSKLEPKKLSVMIKSQGKWSSKLHHMLASSNQID-HLAVSVEGPYGPASTDY 452

Query: 423 LQFDSLIMISGGSGITPFISIIRHLIH-NSNSHQQIPKLLLISAFKNSSDLTFLHLLLPL 482
           L+ DSL+M+SGGSGITPFISIIR L++ +S +  + PK+ LI AFKNSSDL+ L+L+LP 
Sbjct: 453 LRHDSLVMVSGGSGITPFISIIRDLLYVSSTNAYKTPKITLICAFKNSSDLSMLNLILPN 512

Query: 483 S-GANTLENFQIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGIIC 542
           S   ++  + QI+A+VTR+K         +T+ FKP+  D P + ILGPN WLWLA I+ 
Sbjct: 513 STEISSFIDIQIKAFVTREKVSTCNMNIIKTLSFKPYVSDQPISPILGPNSWLWLATILS 572

Query: 543 SSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKRK 602
           SSF IF+ ++ I++RY+IYPID +S + +  +  S +++L + + +V  +T A++ NK+ 
Sbjct: 573 SSFMIFIIIIAIISRYHIYPIDQSSKE-YTSAYTSLIYLLAISISVVATSTVAMLCNKKS 632

Query: 603 LAKEGRQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRILL 662
             K     QNV+  +P              + +ES P + L +  +++YGERPNL ++L+
Sbjct: 633 YFK--GLYQNVDALSP--------------LMIESSPDQLLPEFTNIHYGERPNLNKLLV 692

Query: 663 ERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
             KG +VGVL  GP+KMR+EVA IC  G A NL F+SISF W
Sbjct: 693 GLKGSSVGVLVCGPRKMREEVAKICSFGSAANLQFESISFNW 716

BLAST of CmaCh04G012500 vs. TAIR 10
Match: AT1G23020.2 (ferric reduction oxidase 3 )

HSP 1 Score: 689.1 bits (1777), Expect = 3.6e-198
Identity = 352/703 (50.07%), Postives = 497/703 (70.70%), Query Frame = 0

Query: 3   VLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYTFP 62
           V+   I++L  ++ +G + IW MMPT+TY+  WL  ++ K   S YFG  G +LL+Y FP
Sbjct: 33  VIKNVIKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFP 92

Query: 63  ILFIAVLGCVYVHIERRCREQNVKIR-NRGNDNGLAMWKRPGFVKGPLGIVSWTELALLA 122
           ++ +A LG +Y+H++++ R      R +R   +     KRP  VK  LGIV+ TE+  L 
Sbjct: 93  MILLASLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLM 152

Query: 123 MFVALLVWSFATYLNNSY----------HSENEWESRLGTAAYWLGVVGNICLVFLFFPV 182
           MF+ALL+WS A Y  +++            +N W++RL + A  LG+ GNICL FLF+PV
Sbjct: 153 MFMALLLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNICLGFLFYPV 212

Query: 183 ARGSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDV 242
           ARGSSLL  +GLTSESS KYHIWLG++ M  F++HG+C+ +YW +T+ +SQMLEW +T +
Sbjct: 213 ARGSSLLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQMLEWDRTGI 272

Query: 243 SNVAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPG 302
           S++AGE++LV GL++WATTFP +RR+FFE+F YTH+LY++F++FF+FHVGISYA I  PG
Sbjct: 273 SHLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISYALISFPG 332

Query: 303 FYLFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKL 362
           FY+F++DRFLRFLQSR  V+L+SAR+LPC T+ELNFSK+P L Y+PTS LF+NIP ISKL
Sbjct: 333 FYIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVNIPSISKL 392

Query: 363 QWHPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTS 422
           QWHPFT+TS + LEP+ LSV+IK +G WS+KL+  L+S + ID HL VS+EGPYGP ST 
Sbjct: 393 QWHPFTITSSSKLEPKKLSVMIKSQGKWSSKLHHMLASSNQID-HLAVSVEGPYGPASTD 452

Query: 423 FLQFDSLIMISGGSGITPFISIIRHLIH-NSNSHQQIPKLLLISAFKNSSDLTFLHLLLP 482
           +L+ DSL+M+SGGSGITPFISIIR L++ +S +  + PK+ LI AFKNSSDL+ L+L+LP
Sbjct: 453 YLRHDSLVMVSGGSGITPFISIIRDLLYVSSTNAYKTPKITLICAFKNSSDLSMLNLILP 512

Query: 483 LS-GANTLENFQIEAYVTRDKSPKDENPQFRTVIFKPHEGDSPATAILGPNGWLWLAGII 542
            S   ++  + QI+A+VTR+K         +T+ FKP+  D P + ILGPN WLWLA I+
Sbjct: 513 NSTEISSFIDIQIKAFVTREKVSTCNMNIIKTLSFKPYVSDQPISPILGPNSWLWLATIL 572

Query: 543 CSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIWNKR 602
            SSF IF+ ++ I++RY+IYPID +S + +  +  S +++L + + +V  +T A++ NK+
Sbjct: 573 SSSFMIFIIIIAIISRYHIYPIDQSSKE-YTSAYTSLIYLLAISISVVATSTVAMLCNKK 632

Query: 603 KLAKEGRQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGERPNLPRIL 662
              K     QNV+  +P              + +ES P + L +  +++YGERPNL ++L
Sbjct: 633 SYFK--GLYQNVDALSP--------------LMIESSPDQLLPEFTNIHYGERPNLNKLL 692

Query: 663 LERKGENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           +  KG +VGVL  GP+KMR+EVA IC  G A NL F+SISF W
Sbjct: 693 VGLKGSSVGVLVCGPRKMREEVAKICSFGSAANLQFESISFNW 717

BLAST of CmaCh04G012500 vs. TAIR 10
Match: AT5G23980.1 (ferric reduction oxidase 4 )

HSP 1 Score: 647.1 bits (1668), Expect = 1.6e-185
Identity = 346/712 (48.60%), Postives = 483/712 (67.84%), Query Frame = 0

Query: 1   MGVLSLAIRVLVFILFVGWLFIWFMMPTNTYRHQWLPKIQDKSRSSSYFGSEGTSLLMYT 60
           MG +   ++ L+ +LF+GW+ +W M+ TN ++ +W PK+  K  +++YFG +GT+L++ T
Sbjct: 1   MGNMRSLVKTLMVVLFLGWILVWIMISTNLFKSKWTPKL-SKYLNTTYFGPQGTNLVLLT 60

Query: 61  FPILFIAVLGCVYVHIERRCREQNVKIRNRGNDNGLAMWKRPGFVKGPLGIVSWTELALL 120
            P++FIAVL CVY+HI+++  +   + + +       +  R   V  PLGIV+ TEL   
Sbjct: 61  VPMMFIAVLSCVYLHIQKKPTQPQREWKLK------RIMGRVIMVMNPLGIVTATELTFS 120

Query: 121 AMFVALLVWSFATYLNNSYH-------SENEWESRLGTAAYWLGVVGNICLVFLFFPVAR 180
            +FVALL WS   YL  SYH       +   W+++       +G VGNIC  FLFFPV R
Sbjct: 121 LLFVALLAWSLYNYLYLSYHVHLHNDDNAKIWQAKFRAFGLRIGYVGNICWAFLFFPVTR 180

Query: 181 GSSLLPLLGLTSESSIKYHIWLGHMTMAFFSAHGICFVVYWAATHNLSQMLEWAKTDVSN 240
            S++LPL+GLTSESSIKYHIWLGH++   F  H + F++YWA  + L +   W  T V N
Sbjct: 181 ASTILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLMETFAWNPTYVPN 240

Query: 241 VAGEVSLVCGLIIWATTFPGVRRKFFELFLYTHHLYILFIVFFIFHVGISYACIMLPGFY 300
           +AG +++V G+ +W T+ P  RRK FE+F YTHHLY L+IVF++ HVG S+ C++LP  +
Sbjct: 241 LAGTIAMVIGIAMWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYVIHVGDSWFCMILPNIF 300

Query: 301 LFLLDRFLRFLQSRRRVRLLSARLLPCHTLELNFSKHPGLKYTPTSTLFLNIPCISKLQW 360
           LF +DR+LRFLQS +R RL+SAR+LP   LEL FSK PGL YTPTS LFL++P ISK+QW
Sbjct: 301 LFFIDRYLRFLQSTKRSRLVSARILPSDNLELTFSKTPGLHYTPTSILFLHVPSISKIQW 360

Query: 361 HPFTVTSHADLEPETLSVVIKCEGTWSTKLYQTLSSPSAIDNHLQVSLEGPYGPVSTSFL 420
           HPFT+TS ++LE +TLSVVI+ +G+W+ KLY  LS  S+ID+ L+VS EGPYGP S    
Sbjct: 361 HPFTITSSSNLEKDTLSVVIRRQGSWTQKLYTHLS--SSIDS-LEVSTEGPYGPNSFDVS 420

Query: 421 QFDSLIMISGGSGITPFISIIRHLIHNS-NSHQQIPKLLLISAFKNSSDLTFLHLLLPLS 480
           + +SLI++SGGSGITPFIS+IR LI  S N   ++P +LL+ +FK+  DL FL L+ PL 
Sbjct: 421 RHNSLILVSGGSGITPFISVIRELISQSQNKSTKLPDVLLVCSFKHYHDLAFLDLIFPLD 480

Query: 481 -GANTLE--NFQIEAYVTR-DKSPK--DENPQFRTVIFKPHEGDSPATAILGPNGWLWLA 540
             A+ +   N +IEAY+TR DK P+  D++   +T  FKP   DSP + +LGPN +LWL 
Sbjct: 481 MSASDISRLNLRIEAYITREDKKPETTDDHRLLQTKWFKPQPLDSPISPVLGPNNFLWLG 540

Query: 541 GIICSSFGIFLALMGIVTRYYIYPIDGNSNDVFNLSLRSFLHMLGLCLCIVMAATAAVIW 600
            +I SSF +FL L+GIVTRYYIYP+D N+  ++N S R    M     CI ++++   +W
Sbjct: 541 VVILSSFVMFLLLIGIVTRYYIYPVDHNTGSIYNFSYRGLWDMFLGSACIFISSSVVFLW 600

Query: 601 NKRKLAKEG-----RQIQNVEGATPNASPSAMGFDNVGEMELESVPFEALAQSVHVYYGE 660
            K K  KEG      Q+Q+VE  TP +SP +    +  E ELESVP++++ Q+  V++G 
Sbjct: 601 RK-KQNKEGDKEFKNQVQSVEFQTPTSSPGSWFHGH--ERELESVPYQSIVQATSVHFGS 660

Query: 661 RPNLPRILLERKG-ENVGVLASGPKKMRQEVAAICGSGLAPNLHFQSISFTW 693
           +PNL +ILLE +G E+VGV+  GP+KMR EVA IC SGLA NLHF++ISF W
Sbjct: 661 KPNLKKILLEAEGSEDVGVMVCGPRKMRHEVAKICSSGLAKNLHFEAISFNW 699

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P929491.6e-21954.11Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2[more]
Q9LMM23.4e-20150.91Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2... [more]
F4I4K75.1e-19750.07Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO... [more]
Q8W1102.2e-18448.60Ferric reduction oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=FRO4 PE=2 SV=1[more]
Q9FLW21.2e-18248.00Ferric reduction oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=FRO5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KFY30.0e+00100.00ferric reduction oxidase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1K9Q40.0e+0099.71ferric reduction oxidase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GZA50.0e+0098.41ferric reduction oxidase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1GY090.0e+0098.12ferric reduction oxidase 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S3BD760.0e+0076.77ferric reduction oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103488760 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
XP_022999034.10.0e+00100.00ferric reduction oxidase 2-like isoform X1 [Cucurbita maxima][more]
XP_022999042.10.0e+0099.71ferric reduction oxidase 2-like isoform X2 [Cucurbita maxima][more]
XP_022956524.10.0e+0098.41ferric reduction oxidase 2-like isoform X1 [Cucurbita moschata][more]
XP_022956525.10.0e+0098.12ferric reduction oxidase 2-like isoform X2 [Cucurbita moschata][more]
KAG6601053.10.0e+0097.98Ferric reduction oxidase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G01580.11.2e-22054.11ferric reduction oxidase 2 [more]
AT1G01590.12.4e-20250.91ferric reduction oxidase 1 [more]
AT1G23020.19.5e-19950.00ferric reduction oxidase 3 [more]
AT1G23020.23.6e-19850.07ferric reduction oxidase 3 [more]
AT5G23980.11.6e-18548.60ferric reduction oxidase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000778Cytochrome b245, heavy chainPRINTSPR00466GP91PHOXcoord: 448..461
score: 28.57
coord: 259..279
score: 47.62
coord: 234..257
score: 25.0
IPR013112FAD-binding 8PFAMPF08022FAD_binding_8coord: 309..408
e-value: 2.0E-20
score: 72.9
IPR013121Ferric reductase, NAD binding domainPFAMPF08030NAD_binding_6coord: 415..675
e-value: 2.1E-23
score: 83.2
IPR013130Ferric reductase transmembrane component-like domainPFAMPF01794Ferric_reductcoord: 156..276
e-value: 5.4E-18
score: 65.3
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 399..518
e-value: 5.1E-15
score: 57.8
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 399..502
NoneNo IPR availableSFLDSFLDG01168Ferric_reductase_subgroup_(FRcoord: 11..692
e-value: 0.0
score: 423.3
NoneNo IPR availableSFLDSFLDS00052Ferric_Reductase_Domaincoord: 26..692
e-value: 0.0
score: 442.9
NoneNo IPR availablePANTHERPTHR11972:SF41FERRIC REDUCTION OXIDASE 2coord: 6..691
NoneNo IPR availablePANTHERPTHR11972NADPH OXIDASEcoord: 6..691
NoneNo IPR availableCDDcd06186NOX_Duox_like_FAD_NADPcoord: 312..488
e-value: 2.17469E-39
score: 142.444
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 305..411
score: 11.992607
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 308..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G012500.1CmaCh04G012500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity