CmaCh04G012490 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G012490
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDUF4283 domain-containing protein
LocationCma_Chr04: 6404833 .. 6406587 (-)
RNA-Seq ExpressionCmaCh04G012490
SyntenyCmaCh04G012490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTTCAATCCGACTACCAACTCACTGGCGCCGGCGACGATGAAGCCGCCGCCAGAAACAGGAAAAAGGCCATCGTCTGTGAGCTCTCTGCCTCTCAGACTGCTCGTATTACTCAACAGTTCGATCACTCTTTCATTGCTTGGATCTTCGGAAAGGACGTCCGTCCATGGAGAATCGCGTCTCTTCTTCGCCGTCATCTTTGTCTCACCGGAACTGTTAAGGTCTTTGAGCTAGGACTTGGCTATTTCGTGCTTAAATTCTGTGAGACTGACTTTTTAGCTTTACAAGACGTTCCGTGGTCAGTTCCTAATCTCTGCATCCATGTGTCTCCATGGACTCCTGATTTCAAGCCATCGGAAACCATCCTTTCCTCTGTTGATGTTTGGGTTCGCCTACATGAGCTCTCCATCGAGTATTACGACGACGAAGTTCTGCAAAAAATTGCAGCGGCCATCGGCGGCGATCTTGTGAAGATCGATCCGGTAACTAAAAACCGCTTGAAATGTAAGTTCGCTCGTATTTGTGTTCGAGTAAATTTGTGTGATCCTCTTCCATCGATGATTAGACTTGGTAAAATTAGGCAAGAAATTGAGTATGAAGGTTTTGAGTTGCTGTGCCCTAATTGTAGCCGTGTTGATGGTTTGAGACATAATTGTTTGAATTTGAAAATTCCTTCTGGTTTTTCTGGATTCAATCCCCATAGAGATAAACCCCACCACCATGGAGCTCGTTCTTTTAAGCAGCCGTTGATTCCTTCTGAATCTTCAGCACCATCACCTCGGGGATCCAGATTCCAAGTTCTTGATTTGAATTCGAATGAGTGGCCTAGCCTTGGTGAATCTGGAAAAGCAGGTACGAGTATAAGAACAAGTTCTTCACCTGTTCATGTGAAAGATAAAGCAATTGCAAAGAAAAAGGAGAAATGTGGAGACTCTGTTCAACCATTGCCTAAAGAATCTTCAAGAGTTACCATTAAAGATCAGTTGAAGGCTGCAAAAACCAGCAACAACCCCACCGTGAATGAGCAACCCCCATCACCAACTCCATGCATTCTACCCCTGCAACCATGTCCTGCTTCTGAGGCTGTCCTCGAGTTCCATTCAGCTGCCATCCAACGCTCGACGAGAACGAAAGAGATAACCAGCGTGCCGTCTAAAGAGATCAGTGTCGATAGTCGCCCCACTGTTTACACGATCGACCCGAAGATCACTAGCCTTGACATTGCTTTATCAGAGACACGAACAACCTCAATGCCAAACCAAATTCAATATGCAATCGAGTTCGTTCCGACTATGAGAGATGGCAACAAGGGCGGAGTTGATTTGGGATCAGAATCATGTGGTAAGAAGATTTTGTGCTGGAAGTTTCATTGGACTGACAATGAGAAGCTAATACGAGCATTGAAAGATTTGATTAAGCTACACGAGCCAAGCATTGTGCTGATATTTGGCACCAAGATCAGTGGTGCTGATGTGGATAAGGTAGTGCAGGAGCTCCCTTTCTGCTATTCTTACTACAGAAAGCCTGATGGCTACAATGGTGGGGTTTGGCTGTTATTGTCTAACCAAGATGTTGAAACTAAAGTGAACTCATGCAGCCCACAACAGGTTTGTGCATCAATATATTTTGATTCTAAAACCACTGTAGCAGCGTTTAATCCTTCAGGTGTGGACACCAAAACGTCGTCGGGACCGTGGGGGTCGCCGTTCTTCCATACTCCGACGAACTGGATGACTTCAATGGCATACTGA

mRNA sequence

ATGGCGCTTCAATCCGACTACCAACTCACTGGCGCCGGCGACGATGAAGCCGCCGCCAGAAACAGGAAAAAGGCCATCGTCTGTGAGCTCTCTGCCTCTCAGACTGCTCGTATTACTCAACAGTTCGATCACTCTTTCATTGCTTGGATCTTCGGAAAGGACGTCCGTCCATGGAGAATCGCGTCTCTTCTTCGCCGTCATCTTTGTCTCACCGGAACTGTTAAGGTCTTTGAGCTAGGACTTGGCTATTTCGTGCTTAAATTCTGTGAGACTGACTTTTTAGCTTTACAAGACGTTCCGTGGTCAGTTCCTAATCTCTGCATCCATGTGTCTCCATGGACTCCTGATTTCAAGCCATCGGAAACCATCCTTTCCTCTGTTGATGTTTGGGTTCGCCTACATGAGCTCTCCATCGAGTATTACGACGACGAAGTTCTGCAAAAAATTGCAGCGGCCATCGGCGGCGATCTTGTGAAGATCGATCCGGTAACTAAAAACCGCTTGAAATGTAAGTTCGCTCGTATTTGTGTTCGAGTAAATTTGTGTGATCCTCTTCCATCGATGATTAGACTTGGTAAAATTAGGCAAGAAATTGAGTATGAAGGTTTTGAGTTGCTGTGCCCTAATTGTAGCCGTGTTGATGGTTTGAGACATAATTGTTTGAATTTGAAAATTCCTTCTGGTTTTTCTGGATTCAATCCCCATAGAGATAAACCCCACCACCATGGAGCTCGTTCTTTTAAGCAGCCGTTGATTCCTTCTGAATCTTCAGCACCATCACCTCGGGGATCCAGATTCCAAGTTCTTGATTTGAATTCGAATGAGTGGCCTAGCCTTGGTGAATCTGGAAAAGCAGGTACGAGTATAAGAACAAGTTCTTCACCTGTTCATGTGAAAGATAAAGCAATTGCAAAGAAAAAGGAGAAATGTGGAGACTCTGTTCAACCATTGCCTAAAGAATCTTCAAGAGTTACCATTAAAGATCAGTTGAAGGCTGCAAAAACCAGCAACAACCCCACCGTGAATGAGCAACCCCCATCACCAACTCCATGCATTCTACCCCTGCAACCATGTCCTGCTTCTGAGGCTGTCCTCGAGTTCCATTCAGCTGCCATCCAACGCTCGACGAGAACGAAAGAGATAACCAGCGTGCCGTCTAAAGAGATCAGTGTCGATAGTCGCCCCACTGTTTACACGATCGACCCGAAGATCACTAGCCTTGACATTGCTTTATCAGAGACACGAACAACCTCAATGCCAAACCAAATTCAATATGCAATCGAGTTCGTTCCGACTATGAGAGATGGCAACAAGGGCGGAGTTGATTTGGGATCAGAATCATGTGGTAAGAAGATTTTGTGCTGGAAGTTTCATTGGACTGACAATGAGAAGCTAATACGAGCATTGAAAGATTTGATTAAGCTACACGAGCCAAGCATTGTGCTGATATTTGGCACCAAGATCAGTGGTGCTGATGTGGATAAGGTAGTGCAGGAGCTCCCTTTCTGCTATTCTTACTACAGAAAGCCTGATGGCTACAATGGTGGGGTTTGGCTGTTATTGTCTAACCAAGATGTTGAAACTAAAGTGAACTCATGCAGCCCACAACAGGTTTGTGCATCAATATATTTTGATTCTAAAACCACTGTAGCAGCGTTTAATCCTTCAGGTGTGGACACCAAAACGTCGTCGGGACCGTGGGGGTCGCCGTTCTTCCATACTCCGACGAACTGGATGACTTCAATGGCATACTGA

Coding sequence (CDS)

ATGGCGCTTCAATCCGACTACCAACTCACTGGCGCCGGCGACGATGAAGCCGCCGCCAGAAACAGGAAAAAGGCCATCGTCTGTGAGCTCTCTGCCTCTCAGACTGCTCGTATTACTCAACAGTTCGATCACTCTTTCATTGCTTGGATCTTCGGAAAGGACGTCCGTCCATGGAGAATCGCGTCTCTTCTTCGCCGTCATCTTTGTCTCACCGGAACTGTTAAGGTCTTTGAGCTAGGACTTGGCTATTTCGTGCTTAAATTCTGTGAGACTGACTTTTTAGCTTTACAAGACGTTCCGTGGTCAGTTCCTAATCTCTGCATCCATGTGTCTCCATGGACTCCTGATTTCAAGCCATCGGAAACCATCCTTTCCTCTGTTGATGTTTGGGTTCGCCTACATGAGCTCTCCATCGAGTATTACGACGACGAAGTTCTGCAAAAAATTGCAGCGGCCATCGGCGGCGATCTTGTGAAGATCGATCCGGTAACTAAAAACCGCTTGAAATGTAAGTTCGCTCGTATTTGTGTTCGAGTAAATTTGTGTGATCCTCTTCCATCGATGATTAGACTTGGTAAAATTAGGCAAGAAATTGAGTATGAAGGTTTTGAGTTGCTGTGCCCTAATTGTAGCCGTGTTGATGGTTTGAGACATAATTGTTTGAATTTGAAAATTCCTTCTGGTTTTTCTGGATTCAATCCCCATAGAGATAAACCCCACCACCATGGAGCTCGTTCTTTTAAGCAGCCGTTGATTCCTTCTGAATCTTCAGCACCATCACCTCGGGGATCCAGATTCCAAGTTCTTGATTTGAATTCGAATGAGTGGCCTAGCCTTGGTGAATCTGGAAAAGCAGGTACGAGTATAAGAACAAGTTCTTCACCTGTTCATGTGAAAGATAAAGCAATTGCAAAGAAAAAGGAGAAATGTGGAGACTCTGTTCAACCATTGCCTAAAGAATCTTCAAGAGTTACCATTAAAGATCAGTTGAAGGCTGCAAAAACCAGCAACAACCCCACCGTGAATGAGCAACCCCCATCACCAACTCCATGCATTCTACCCCTGCAACCATGTCCTGCTTCTGAGGCTGTCCTCGAGTTCCATTCAGCTGCCATCCAACGCTCGACGAGAACGAAAGAGATAACCAGCGTGCCGTCTAAAGAGATCAGTGTCGATAGTCGCCCCACTGTTTACACGATCGACCCGAAGATCACTAGCCTTGACATTGCTTTATCAGAGACACGAACAACCTCAATGCCAAACCAAATTCAATATGCAATCGAGTTCGTTCCGACTATGAGAGATGGCAACAAGGGCGGAGTTGATTTGGGATCAGAATCATGTGGTAAGAAGATTTTGTGCTGGAAGTTTCATTGGACTGACAATGAGAAGCTAATACGAGCATTGAAAGATTTGATTAAGCTACACGAGCCAAGCATTGTGCTGATATTTGGCACCAAGATCAGTGGTGCTGATGTGGATAAGGTAGTGCAGGAGCTCCCTTTCTGCTATTCTTACTACAGAAAGCCTGATGGCTACAATGGTGGGGTTTGGCTGTTATTGTCTAACCAAGATGTTGAAACTAAAGTGAACTCATGCAGCCCACAACAGGTTTGTGCATCAATATATTTTGATTCTAAAACCACTGTAGCAGCGTTTAATCCTTCAGGTGTGGACACCAAAACGTCGTCGGGACCGTGGGGGTCGCCGTTCTTCCATACTCCGACGAACTGGATGACTTCAATGGCATACTGA

Protein sequence

MALQSDYQLTGAGDDEAAARNRKKAIVCELSASQTARITQQFDHSFIAWIFGKDVRPWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQDVPWSVPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNPHRDKPHHHGARSFKQPLIPSESSAPSPRGSRFQVLDLNSNEWPSLGESGKAGTSIRTSSSPVHVKDKAIAKKKEKCGDSVQPLPKESSRVTIKDQLKAAKTSNNPTVNEQPPSPTPCILPLQPCPASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDPKITSLDIALSETRTTSMPNQIQYAIEFVPTMRDGNKGGVDLGSESCGKKILCWKFHWTDNEKLIRALKDLIKLHEPSIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCSPQQVCASIYFDSKTTVAAFNPSGVDTKTSSGPWGSPFFHTPTNWMTSMAY
Homology
BLAST of CmaCh04G012490 vs. ExPASy TrEMBL
Match: A0A5A7SSJ3 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00480 PE=4 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 6.2e-207
Identity = 406/650 (62.46%), Postives = 460/650 (70.77%), Query Frame = 0

Query: 1   MALQSDYQLTGAGDDEAAARN---RKKAIV-----------------------CELSASQ 60
           MALQSD+  TGAGDDEAAARN   RKK  V                       C L+ S+
Sbjct: 1   MALQSDHPPTGAGDDEAAARNYLSRKKPKVPPPISPSSDFESRPSTTIATVCNCNLTPSE 60

Query: 61  TARITQQFDHSFIAWIFGKDVRPWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFL 120
           T RITQQF HS IA + GKD RP ++A+ LR HL LT  VKVFELGLGYFVLKF ETD+L
Sbjct: 61  TTRITQQFIHSLIARVVGKDTRPGQLAARLRHHLRLTQDVKVFELGLGYFVLKFSETDYL 120

Query: 121 ALQDVPWSVPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIG 180
           AL+D+PWS+PNLCIH  PWTPDFKPSE I SSV+VW+RL ELSIEYYD E+L++IA AIG
Sbjct: 121 ALEDLPWSIPNLCIHAFPWTPDFKPSEAINSSVNVWIRLPELSIEYYDVEILKRIADAIG 180

Query: 181 GDLVKIDPVTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVD 240
           G LVKIDPVT++R KCKFAR C+ VNLCDPLPSMI LG+IRQ IEYEGFE LC  C+RV 
Sbjct: 181 GRLVKIDPVTRDRWKCKFARFCISVNLCDPLPSMIELGRIRQRIEYEGFE-LCAKCNRVG 240

Query: 241 GLRHNCLNLKIPSGFSGFNPHRDKPHHHGARSF---------KQPLIPSESSAPSPRGSR 300
            LRH+C +L  PSG  GFNPH D+PHH   R F         KQPLIP  S   +   SR
Sbjct: 241 DLRHDCSSLNNPSGSYGFNPHGDEPHHSVTRYFKEFGSTSSSKQPLIPESSRVSAWESSR 300

Query: 301 F----QVLDLNSNEWPSL--GESGKAGTSIRTSSSPVHVKDKAIAKKKEKCGDSVQPLP- 360
           F      LDL S  WP+L   ESGKAGTS+R SS  VHVKDKAI KKKEKC  SVQPLP 
Sbjct: 301 FIEKNPQLDLKSINWPNLPKSESGKAGTSVRISSPHVHVKDKAIPKKKEKCEISVQPLPS 360

Query: 361 ---KESSRVTIK-------------DQLKAAKTSNNPTV---NEQPPSPTPCILPLQPCP 420
              ++SS +TIK             DQLK AKT N+  +   N QPPSPT  I  LQP P
Sbjct: 361 LPKQQSSTITIKAPELKCVVPSVVEDQLKDAKTINSTMIADHNSQPPSPTASIPFLQPSP 420

Query: 421 ASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDP-KITSLDIALSETRTTS 480
           ASEA L+F S AI   TR +EI + PSKE +  S PTVYTIDP KITSL+I+LSE +TTS
Sbjct: 421 ASEATLKFLSDAILCLTRKEEICNSPSKETNDSSFPTVYTIDPKKITSLNISLSEVQTTS 480

Query: 481 MPNQIQYAIEFVPTMRDGNKGGVDL----GSESCGKKILCWKFHWTDNEKLIRALKDLIK 540
           M NQ QY IE VPTM+ G+KGGV L    GSE C KK+L WKFH  DN KL+RALKDLI+
Sbjct: 481 MSNQNQYTIELVPTMKGGDKGGVGLEVESGSEPCAKKMLVWKFHAMDNAKLMRALKDLIQ 540

Query: 541 LHEPSIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCS 585
           LHEPSIVLIFG KI+G D  KV+QEL FC SY  +PDGYNGGVWLLLS QDV+TKVNS S
Sbjct: 541 LHEPSIVLIFGNKITGVDAVKVMQELAFCGSYSSRPDGYNGGVWLLLSKQDVQTKVNSYS 600

BLAST of CmaCh04G012490 vs. ExPASy TrEMBL
Match: A0A0A0KLB0 (DUF4283 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175790 PE=4 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 2.1e-191
Identity = 388/659 (58.88%), Postives = 444/659 (67.37%), Query Frame = 0

Query: 1   MALQSDYQLTGAGDDEAAARN---RKKAIV-----------------------CELSASQ 60
           MA QS +  TGAGDDEAAARN   RKK  V                       C L+ S+
Sbjct: 1   MAFQSGHPPTGAGDDEAAARNYLSRKKPKVPPPIPPSSDFHSRRSTTIATVCNCNLTPSE 60

Query: 61  TARITQQFDHSFIAWIFGKDVRPWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFL 120
           T RITQQF HS IA + GKD RP ++A+ LR HL LT  VKVF+LGLGYFVLKF ETD+L
Sbjct: 61  TTRITQQFVHSLIARVVGKDTRPGQLAARLRHHLRLTQDVKVFQLGLGYFVLKFSETDYL 120

Query: 121 ALQDVPWSVPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIG 180
           AL+D+PWS+PNLCIH  PWTPDFKPSE I SSV+VW+RL ELSIEYYD  +L++IA AIG
Sbjct: 121 ALEDLPWSIPNLCIHAFPWTPDFKPSEAINSSVNVWIRLPELSIEYYDVGILKRIADAIG 180

Query: 181 GDLVKIDPVTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVD 240
             LVKIDPVT++R KCKFAR C+ VNLCDPLPSMI LG++RQ IEYEGFE LC  C+RV 
Sbjct: 181 DPLVKIDPVTRDRWKCKFARFCISVNLCDPLPSMIELGRVRQRIEYEGFE-LCAKCNRVG 240

Query: 241 GLRHNCL----------NLKIPSGFSGFNPHRDKPHHHGARSF---------KQPLIPSE 300
            LRH+C           +L  PSG  GFNPH D+PHH   R F         KQPLIP  
Sbjct: 241 DLRHDCSSLNNPSLNNPSLNNPSGSYGFNPHGDEPHHSVTRDFKEIGSTSNSKQPLIPES 300

Query: 301 SSAPSPRGSRF----QVLDLNSNEWPSL--GESGKAGTSIRTSSSPVHVKDKAIAKKKEK 360
           S   +   SRF      LDL   +WP+L   ESGKAG+ +R SS  VHVKDK I KKKEK
Sbjct: 301 SPVSAWESSRFIEKNPPLDLKLIDWPNLPKRESGKAGSGVRISSPRVHVKDKEIPKKKEK 360

Query: 361 CGDSVQ---PLPKESSRVTIK-------------DQLKAAKTSNNPTV---NEQPPSPTP 420
           C  SVQ    LPK+ S +TIK             D+LK  KT N+  +   N QPPSPT 
Sbjct: 361 CEISVQRLPNLPKQCSTITIKAPELKRVVPSVVEDRLKDTKTINSTMIADHNSQPPSPTA 420

Query: 421 CILPLQPCPASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDP-KITSLDI 480
            I  LQP PASEA L+F S AI   TR +EI + PSK I+  S PTVYTIDP KITSL+I
Sbjct: 421 SIPFLQPSPASEATLKFLSDAILCLTRKEEICNSPSKVINDSSFPTVYTIDPKKITSLNI 480

Query: 481 ALSETRTTSMPNQIQYAIEFVPTMRDGNKGG----VDLGSESCGKKILCWKFHWTDNEKL 540
           ALSE +T          IE VPTM+ G++GG    V+ GSE C KKIL WKFH  DN KL
Sbjct: 481 ALSEVQT----------IELVPTMKGGDEGGVGSEVESGSEPCAKKILVWKFHVMDNAKL 540

Query: 541 IRALKDLIKLHEPSIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQD 585
           +RALKDLI+LHEPSIVLIFG KISG D DKV++EL FC SY  KPDGYNGGVWLLLS QD
Sbjct: 541 MRALKDLIQLHEPSIVLIFGNKISGVDTDKVMRELAFCGSYSSKPDGYNGGVWLLLSKQD 600

BLAST of CmaCh04G012490 vs. ExPASy TrEMBL
Match: A0A6J1FN13 (uncharacterized protein LOC111446932 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446932 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.7e-135
Identity = 299/604 (49.50%), Postives = 370/604 (61.26%), Query Frame = 0

Query: 1   MALQSDY----QLTGAGDDEAAARNRKKAIVCELSASQTARITQQFDHSFIAWIFGKDVR 60
           MA+QS +    + TGAG+D AAA +   A VC L+ SQTARI QQFD S I W+ GK + 
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAA-STIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIH 60

Query: 61  PWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCET--DFLALQDVPWSVPNLCIHVSPWT 120
           P ++A  LRR+L L G + VFELGLG+FVLKF      + AL++ PWS+P+LCI+V PW 
Sbjct: 61  PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWI 120

Query: 121 PDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFAR 180
           P+FKPSE  +  VDVW+RL ELSIEYYD EVL+KIA  IGG LVKIDPVT  R KC +AR
Sbjct: 121 PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTVTREKCMYAR 180

Query: 181 ICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNP 240
           IC+R+NL  PL    + GK  Q+I YEG +LLC  C  VD L+H+CL+ +  S  SGF+ 
Sbjct: 181 ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNR--SSSSGFD- 240

Query: 241 HRDKPHHHGAR-------------------------------SFKQPLIPSESSAPSPRG 300
               PHHH AR                               + K  LIPS+ +  S RG
Sbjct: 241 ----PHHHSARPLQATGSSLSSNVNPCSSSNLNQNPSSSSNSNLKMQLIPSKPAPASARG 300

Query: 301 SRFQVLDLNSNEWPSLGESGKAGTSIRTSSSPVHVKDKAIAKKKEKCGDSVQPLPKESSR 360
           SRFQVL+LN NE PSL               PV   DK +   KE    ++ PL K+++ 
Sbjct: 301 SRFQVLELNLNEEPSL---------------PVSESDKEV---KESPSITMNPLLKQTNL 360

Query: 361 V--------TIKD-QLKAAKTSNNPTVNEQPPSPTP------CILPLQPCPASEAVLEFH 420
           +         ++D Q +  KTS+  T+  Q   P P       I PLQP  A EA L+F+
Sbjct: 361 IQSVPLAPCVLEDHQFRTEKTSSPTTLAVQNNEPQPSSLAIKSIAPLQPSSALEAGLKFY 420

Query: 421 SAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDPKITSLDIALSE-TRTTSMPNQIQYAI 480
           S AIQ+ST  K I + PS+ ISVDS PT+YTIDP ITSL I L E + TT+  NQ ++AI
Sbjct: 421 STAIQQSTIQKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAI 480

Query: 481 EFVPTMRDGNKGGVDLGSESCGKKILCWKFHWTDNEKLIRALKDLIKLHEPSIVLIFGTK 540
             VPT        V + +  C KK+LCW F  TDN KL+RALKDLI+LH+PSIVLIFGTK
Sbjct: 481 HIVPTSE-----AVSMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTK 540

Query: 541 ISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCSPQQVCASIYFDSK 552
           ISGAD D VV+EL F  SY RKPDGY GG WLLLS QDV+ +V+S SPQQV AS+   SK
Sbjct: 541 ISGADADHVVRELAFDGSYCRKPDGYKGGAWLLLSQQDVQIEVSSYSPQQVSASVILHSK 573

BLAST of CmaCh04G012490 vs. ExPASy TrEMBL
Match: A0A6J1FU80 (uncharacterized protein LOC111446932 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446932 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 2.8e-135
Identity = 299/606 (49.34%), Postives = 370/606 (61.06%), Query Frame = 0

Query: 1   MALQSDY----QLTGAGDDEAAARNRKKAIVCELSASQTARITQQFDHSFIAWIFGKDVR 60
           MA+QS +    + TGAG+D AAA +   A VC L+ SQTARI QQFD S I W+ GK + 
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAA-STIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIH 60

Query: 61  PWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCET--DFLALQDVPWSVPNLCIHVSPWT 120
           P ++A  LRR+L L G + VFELGLG+FVLKF      + AL++ PWS+P+LCI+V PW 
Sbjct: 61  PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWI 120

Query: 121 PDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFAR 180
           P+FKPSE  +  VDVW+RL ELSIEYYD EVL+KIA  IGG LVKIDPVT  R KC +AR
Sbjct: 121 PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTVTREKCMYAR 180

Query: 181 ICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNP 240
           IC+R+NL  PL    + GK  Q+I YEG +LLC  C  VD L+H+CL+ +  S  SGF+ 
Sbjct: 181 ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNR--SSSSGFD- 240

Query: 241 HRDKPHHHGAR---------------------------------SFKQPLIPSESSAPSP 300
               PHHH AR                                 + K  LIPS+ +  S 
Sbjct: 241 ----PHHHSARPLQATGSSLSSNVNPCSSSNLNPNLSSSSNSNSNLKMQLIPSKPAPASA 300

Query: 301 RGSRFQVLDLNSNEWPSLGESGKAGTSIRTSSSPVHVKDKAIAKKKEKCGDSVQPLPKES 360
           RGSRFQVL+LN NE PSL               PV   DK +   KE    ++ PL K++
Sbjct: 301 RGSRFQVLELNLNEEPSL---------------PVSESDKEV---KESPSITMNPLLKQT 360

Query: 361 SRV--------TIKD-QLKAAKTSNNPTVNEQPPSPTP------CILPLQPCPASEAVLE 420
           + +         ++D Q +  KTS+  T+  Q   P P       I PLQP  A EA L+
Sbjct: 361 NLIQSVPLAPCVLEDHQFRTEKTSSPTTLAVQNNEPQPSSLAIKSIAPLQPSSALEAGLK 420

Query: 421 FHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDPKITSLDIALSE-TRTTSMPNQIQY 480
           F+S AIQ+ST  K I + PS+ ISVDS PT+YTIDP ITSL I L E + TT+  NQ ++
Sbjct: 421 FYSTAIQQSTIQKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEH 480

Query: 481 AIEFVPTMRDGNKGGVDLGSESCGKKILCWKFHWTDNEKLIRALKDLIKLHEPSIVLIFG 540
           AI  VPT        V + +  C KK+LCW F  TDN KL+RALKDLI+LH+PSIVLIFG
Sbjct: 481 AIHIVPTSE-----AVSMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFG 540

Query: 541 TKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCSPQQVCASIYFD 552
           TKISGAD D VV+EL F  SY RKPDGY GG WLLLS QDV+ +V+S SPQQV AS+   
Sbjct: 541 TKISGADADHVVRELAFDGSYCRKPDGYKGGAWLLLSQQDVQIEVSSYSPQQVSASVILH 575

BLAST of CmaCh04G012490 vs. ExPASy TrEMBL
Match: A0A5A7SUD3 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00490 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 3.1e-121
Identity = 282/653 (43.19%), Postives = 368/653 (56.36%), Query Frame = 0

Query: 5   SDYQLTGAGDDEAAARN---RKK-------------------AIVCELSASQTARITQQF 64
           S +Q TGAG DEAA+RN   RKK                   + VC+ SASQT  I ++F
Sbjct: 7   SPHQPTGAGSDEAASRNHGSRKKSKQPISHSPDFNSLHSTTRSTVCKFSASQTDLIAREF 66

Query: 65  DHSFIAWIFGKDVRPWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQDVPWS 124
            HS IAW+ GK++RP R+A  L RHL LT    VFELGLGYFVLKFCETDFLAL+D PW 
Sbjct: 67  AHSLIAWVVGKEIRPLRLARHLHRHLRLTELPDVFELGLGYFVLKFCETDFLALEDNPWP 126

Query: 125 VPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDP 184
           +PNLCI+  PWTP+FKPSE + S++D W+RL EL IEYY +++L+ I   +G  LVKIDP
Sbjct: 127 IPNLCIYAFPWTPNFKPSEAMDSAIDCWIRLKELPIEYYKEDILRDIGKTVGEALVKIDP 186

Query: 185 VTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLN 244
           +TK+R KCK+ARICVR+N+ +PLPS IR+GKI QEIEYEGF++LCP C  V  L+H+CLN
Sbjct: 187 ITKDRKKCKYARICVRINVYEPLPSSIRIGKILQEIEYEGFDVLCPRCECVVHLKHDCLN 246

Query: 245 LKIPSGFSGFNPHRDKPHHHGARSFKQPLIPSESSAPSPRGSRFQV---------LDLNS 304
                  SG +   +  H     + KQPL+PSESS     GSRF+V          +L +
Sbjct: 247 ------SSGSSSSFEPDHPRNGSNSKQPLVPSESSV--AWGSRFEVPGTESKSPLQNLKA 306

Query: 305 NEWPSLGESGKAGTSIRTSSSPV-----HVKDKAIAKKKEKCGDSVQPLP---------- 364
              PS+G S KA T  RTSSSP+      + ++ + K+KEKCG S +  P          
Sbjct: 307 LSIPSMGGSEKAAT--RTSSSPLLPQSSGLLNEPLEKQKEKCGGSFEIFPNLPKEDLPQS 366

Query: 365 -------KESSRVTIKDQLKAAKTSN-------NPTVNEQPPSPTPCILPLQ-------- 424
                  +ESS  TI   +   K  N        P  N   P+ T C + L+        
Sbjct: 367 LSISSNLEESSSSTISVPVFEQKNLNLSMVLAPLPAENPFTPAETSCSIKLEVHNNQPQP 426

Query: 425 -------------PCPASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDPK 484
                          P+S+ +  F S+ I RS   K+ITS  S+   ++ RP +YTI   
Sbjct: 427 SSSPLAASISTQPSSPSSKTIPTFCSSGIARSILKKKITSASSQGFGINRRPILYTIPES 486

Query: 485 ITSLDIALSETRTTSMPNQIQYAIEFVPTMRDGNKGGVDLGSESCGKKILCWKFHWTDNE 544
           I S ++ LSE    S P Q Q++I FV T R G K    L S    KK+L W F   DN 
Sbjct: 487 IKSFEVGLSE-NPDSAPKQNQFSISFVSTPRSGTKAISALDS----KKMLGWNFRGMDNV 546

Query: 545 KLIRALKDLIKLHEPSIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSN 573
            LI  L  +++ +EPSIV+IFGT+I+   V++VV +L F  SY +K D Y+GGVWL +  
Sbjct: 547 NLIEGLNYMVQKYEPSIVVIFGTRITDDVVEEVVDKLAFPGSYIKKFDNYHGGVWLFMFR 606

BLAST of CmaCh04G012490 vs. NCBI nr
Match: KAG7031864.1 (hypothetical protein SDJN02_05905, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 555/586 (94.71%), Postives = 566/586 (96.59%), Query Frame = 0

Query: 1   MALQSDYQLTGAGDDE--AAARNRKKAIVCELSASQTARITQQFDHSFIAWIFGKDVRPW 60
           MALQSDYQLTGAGDDE  AAARNRKKA VCELSASQTARITQQFDHSFIAWIFGKDVRPW
Sbjct: 1   MALQSDYQLTGAGDDEAAAAARNRKKATVCELSASQTARITQQFDHSFIAWIFGKDVRPW 60

Query: 61  RIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQDVPWSVPNLCIHVSPWTPDFK 120
           RIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQD+PWSVPNLCIHVSPWTPDFK
Sbjct: 61  RIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQDLPWSVPNLCIHVSPWTPDFK 120

Query: 121 PSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVR 180
           PSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVR
Sbjct: 121 PSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVR 180

Query: 181 VNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNPHRDK 240
           VNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNPHR K
Sbjct: 181 VNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNPHRVK 240

Query: 241 PHHHGARSFKQPLIPSESSAPSPRGSRFQVLDLNSNEWPSLGESGKAGTSIRTSSSPVHV 300
           PHHHGARSFKQPLIPSESSAPSPRGSRFQVLDLNSNEWP+LGESGKAGTSIRTSSSPVHV
Sbjct: 241 PHHHGARSFKQPLIPSESSAPSPRGSRFQVLDLNSNEWPTLGESGKAGTSIRTSSSPVHV 300

Query: 301 KDKAIAKKKEKCGDSVQPLPKESSRVTIKDQLKAAKTSNNPTVNEQPPSPTPCILPLQPC 360
           KDKAIAKKKEKCG SVQPLPKESS+VTIKDQLKAAKTSNNPTVNEQPPSPTP + PL PC
Sbjct: 301 KDKAIAKKKEKCGVSVQPLPKESSKVTIKDQLKAAKTSNNPTVNEQPPSPTPSLPPLLPC 360

Query: 361 PASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDPKITSLDIALSETRTTS 420
           PASEA+L FHSAAIQRSTRTKEIT  PSKEI+VDS PTVYTIDPKI +LDIALSETRTTS
Sbjct: 361 PASEAILNFHSAAIQRSTRTKEITDAPSKEINVDSCPTVYTIDPKIATLDIALSETRTTS 420

Query: 421 MPNQIQYAIEFVPTMRDGNKGGVDLGSESCGKKILCWKFHWTDNEKLIRALKDLIKLHEP 480
           M NQIQYAIEFVPT RDG+KGGVD GSESCGKKILCWKFHWTDNEKLIR+LKDLIKLHEP
Sbjct: 421 MSNQIQYAIEFVPTTRDGDKGGVDSGSESCGKKILCWKFHWTDNEKLIRSLKDLIKLHEP 480

Query: 481 SIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCSPQQV 540
           SIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGY+GGVWLLLSNQDVETKVNSCSPQQ+
Sbjct: 481 SIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYSGGVWLLLSNQDVETKVNSCSPQQL 540

Query: 541 CASIYFDSKTTVAAFNPSGVDTKTSSGPWGSPFFHTPTNWMTSMAY 585
           CASIYFDSKT  AAFNPSGV TK SSGPWGSPFFHTPTNWMTSMAY
Sbjct: 541 CASIYFDSKTNAAAFNPSGVQTKASSGPWGSPFFHTPTNWMTSMAY 586

BLAST of CmaCh04G012490 vs. NCBI nr
Match: KAG6601052.1 (hypothetical protein SDJN03_06285, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 554/586 (94.54%), Postives = 565/586 (96.42%), Query Frame = 0

Query: 1   MALQSDYQLTGAGDDE--AAARNRKKAIVCELSASQTARITQQFDHSFIAWIFGKDVRPW 60
           MALQSDYQLTGAGDDE  AAARNRKKA VCELSASQTARITQQFDHSFIAWIFGKDVRPW
Sbjct: 1   MALQSDYQLTGAGDDEAAAAARNRKKATVCELSASQTARITQQFDHSFIAWIFGKDVRPW 60

Query: 61  RIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQDVPWSVPNLCIHVSPWTPDFK 120
           RIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQD+PWSVPNLCIHVSPWTPDFK
Sbjct: 61  RIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQDLPWSVPNLCIHVSPWTPDFK 120

Query: 121 PSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVR 180
           PSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVR
Sbjct: 121 PSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVR 180

Query: 181 VNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNPHRDK 240
           VNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNPHR K
Sbjct: 181 VNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNCLNLKIPSGFSGFNPHRVK 240

Query: 241 PHHHGARSFKQPLIPSESSAPSPRGSRFQVLDLNSNEWPSLGESGKAGTSIRTSSSPVHV 300
           PHHHGARSFKQPLIPSESSAPSPRGSRFQVLDLNSNEWP+LGESGKAGTSIRTSSSPVHV
Sbjct: 241 PHHHGARSFKQPLIPSESSAPSPRGSRFQVLDLNSNEWPTLGESGKAGTSIRTSSSPVHV 300

Query: 301 KDKAIAKKKEKCGDSVQPLPKESSRVTIKDQLKAAKTSNNPTVNEQPPSPTPCILPLQPC 360
           KDKAIAKKKEKCG SVQPLPKESS+VTIKDQLKAAKTSNNPTVNEQP SPTP + PL PC
Sbjct: 301 KDKAIAKKKEKCGVSVQPLPKESSKVTIKDQLKAAKTSNNPTVNEQPTSPTPSLPPLLPC 360

Query: 361 PASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDPKITSLDIALSETRTTS 420
           PASEA+L FHSAAIQRSTRTKEIT  PSKEI+VDS PTVYTIDPKI +LDIALSETRTTS
Sbjct: 361 PASEAILNFHSAAIQRSTRTKEITDAPSKEINVDSCPTVYTIDPKIATLDIALSETRTTS 420

Query: 421 MPNQIQYAIEFVPTMRDGNKGGVDLGSESCGKKILCWKFHWTDNEKLIRALKDLIKLHEP 480
           M NQIQYAIEFVPT RDG+KGGVD GSESCGKKILCWKFHWTDNEKLIR+LKDLIKLHEP
Sbjct: 421 MSNQIQYAIEFVPTTRDGDKGGVDSGSESCGKKILCWKFHWTDNEKLIRSLKDLIKLHEP 480

Query: 481 SIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCSPQQV 540
           SIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGY+GGVWLLLSNQDVETKVNSCSPQQ+
Sbjct: 481 SIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYSGGVWLLLSNQDVETKVNSCSPQQL 540

Query: 541 CASIYFDSKTTVAAFNPSGVDTKTSSGPWGSPFFHTPTNWMTSMAY 585
           CASIYFDSKT  AAFNPSGV TK SSGPWGSPFFHTPTNWMTSMAY
Sbjct: 541 CASIYFDSKTNAAAFNPSGVQTKASSGPWGSPFFHTPTNWMTSMAY 586

BLAST of CmaCh04G012490 vs. NCBI nr
Match: KAA0034062.1 (uncharacterized protein E6C27_scaffold65G00480 [Cucumis melo var. makuwa])

HSP 1 Score: 730.3 bits (1884), Expect = 1.3e-206
Identity = 406/650 (62.46%), Postives = 460/650 (70.77%), Query Frame = 0

Query: 1   MALQSDYQLTGAGDDEAAARN---RKKAIV-----------------------CELSASQ 60
           MALQSD+  TGAGDDEAAARN   RKK  V                       C L+ S+
Sbjct: 1   MALQSDHPPTGAGDDEAAARNYLSRKKPKVPPPISPSSDFESRPSTTIATVCNCNLTPSE 60

Query: 61  TARITQQFDHSFIAWIFGKDVRPWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFL 120
           T RITQQF HS IA + GKD RP ++A+ LR HL LT  VKVFELGLGYFVLKF ETD+L
Sbjct: 61  TTRITQQFIHSLIARVVGKDTRPGQLAARLRHHLRLTQDVKVFELGLGYFVLKFSETDYL 120

Query: 121 ALQDVPWSVPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIG 180
           AL+D+PWS+PNLCIH  PWTPDFKPSE I SSV+VW+RL ELSIEYYD E+L++IA AIG
Sbjct: 121 ALEDLPWSIPNLCIHAFPWTPDFKPSEAINSSVNVWIRLPELSIEYYDVEILKRIADAIG 180

Query: 181 GDLVKIDPVTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVD 240
           G LVKIDPVT++R KCKFAR C+ VNLCDPLPSMI LG+IRQ IEYEGFE LC  C+RV 
Sbjct: 181 GRLVKIDPVTRDRWKCKFARFCISVNLCDPLPSMIELGRIRQRIEYEGFE-LCAKCNRVG 240

Query: 241 GLRHNCLNLKIPSGFSGFNPHRDKPHHHGARSF---------KQPLIPSESSAPSPRGSR 300
            LRH+C +L  PSG  GFNPH D+PHH   R F         KQPLIP  S   +   SR
Sbjct: 241 DLRHDCSSLNNPSGSYGFNPHGDEPHHSVTRYFKEFGSTSSSKQPLIPESSRVSAWESSR 300

Query: 301 F----QVLDLNSNEWPSL--GESGKAGTSIRTSSSPVHVKDKAIAKKKEKCGDSVQPLP- 360
           F      LDL S  WP+L   ESGKAGTS+R SS  VHVKDKAI KKKEKC  SVQPLP 
Sbjct: 301 FIEKNPQLDLKSINWPNLPKSESGKAGTSVRISSPHVHVKDKAIPKKKEKCEISVQPLPS 360

Query: 361 ---KESSRVTIK-------------DQLKAAKTSNNPTV---NEQPPSPTPCILPLQPCP 420
              ++SS +TIK             DQLK AKT N+  +   N QPPSPT  I  LQP P
Sbjct: 361 LPKQQSSTITIKAPELKCVVPSVVEDQLKDAKTINSTMIADHNSQPPSPTASIPFLQPSP 420

Query: 421 ASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDP-KITSLDIALSETRTTS 480
           ASEA L+F S AI   TR +EI + PSKE +  S PTVYTIDP KITSL+I+LSE +TTS
Sbjct: 421 ASEATLKFLSDAILCLTRKEEICNSPSKETNDSSFPTVYTIDPKKITSLNISLSEVQTTS 480

Query: 481 MPNQIQYAIEFVPTMRDGNKGGVDL----GSESCGKKILCWKFHWTDNEKLIRALKDLIK 540
           M NQ QY IE VPTM+ G+KGGV L    GSE C KK+L WKFH  DN KL+RALKDLI+
Sbjct: 481 MSNQNQYTIELVPTMKGGDKGGVGLEVESGSEPCAKKMLVWKFHAMDNAKLMRALKDLIQ 540

Query: 541 LHEPSIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCS 585
           LHEPSIVLIFG KI+G D  KV+QEL FC SY  +PDGYNGGVWLLLS QDV+TKVNS S
Sbjct: 541 LHEPSIVLIFGNKITGVDAVKVMQELAFCGSYSSRPDGYNGGVWLLLSKQDVQTKVNSYS 600

BLAST of CmaCh04G012490 vs. NCBI nr
Match: KGN50455.1 (hypothetical protein Csa_000357 [Cucumis sativus])

HSP 1 Score: 678.7 bits (1750), Expect = 4.4e-191
Identity = 388/659 (58.88%), Postives = 444/659 (67.37%), Query Frame = 0

Query: 1   MALQSDYQLTGAGDDEAAARN---RKKAIV-----------------------CELSASQ 60
           MA QS +  TGAGDDEAAARN   RKK  V                       C L+ S+
Sbjct: 1   MAFQSGHPPTGAGDDEAAARNYLSRKKPKVPPPIPPSSDFHSRRSTTIATVCNCNLTPSE 60

Query: 61  TARITQQFDHSFIAWIFGKDVRPWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFL 120
           T RITQQF HS IA + GKD RP ++A+ LR HL LT  VKVF+LGLGYFVLKF ETD+L
Sbjct: 61  TTRITQQFVHSLIARVVGKDTRPGQLAARLRHHLRLTQDVKVFQLGLGYFVLKFSETDYL 120

Query: 121 ALQDVPWSVPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIG 180
           AL+D+PWS+PNLCIH  PWTPDFKPSE I SSV+VW+RL ELSIEYYD  +L++IA AIG
Sbjct: 121 ALEDLPWSIPNLCIHAFPWTPDFKPSEAINSSVNVWIRLPELSIEYYDVGILKRIADAIG 180

Query: 181 GDLVKIDPVTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVD 240
             LVKIDPVT++R KCKFAR C+ VNLCDPLPSMI LG++RQ IEYEGFE LC  C+RV 
Sbjct: 181 DPLVKIDPVTRDRWKCKFARFCISVNLCDPLPSMIELGRVRQRIEYEGFE-LCAKCNRVG 240

Query: 241 GLRHNCL----------NLKIPSGFSGFNPHRDKPHHHGARSF---------KQPLIPSE 300
            LRH+C           +L  PSG  GFNPH D+PHH   R F         KQPLIP  
Sbjct: 241 DLRHDCSSLNNPSLNNPSLNNPSGSYGFNPHGDEPHHSVTRDFKEIGSTSNSKQPLIPES 300

Query: 301 SSAPSPRGSRF----QVLDLNSNEWPSL--GESGKAGTSIRTSSSPVHVKDKAIAKKKEK 360
           S   +   SRF      LDL   +WP+L   ESGKAG+ +R SS  VHVKDK I KKKEK
Sbjct: 301 SPVSAWESSRFIEKNPPLDLKLIDWPNLPKRESGKAGSGVRISSPRVHVKDKEIPKKKEK 360

Query: 361 CGDSVQ---PLPKESSRVTIK-------------DQLKAAKTSNNPTV---NEQPPSPTP 420
           C  SVQ    LPK+ S +TIK             D+LK  KT N+  +   N QPPSPT 
Sbjct: 361 CEISVQRLPNLPKQCSTITIKAPELKRVVPSVVEDRLKDTKTINSTMIADHNSQPPSPTA 420

Query: 421 CILPLQPCPASEAVLEFHSAAIQRSTRTKEITSVPSKEISVDSRPTVYTIDP-KITSLDI 480
            I  LQP PASEA L+F S AI   TR +EI + PSK I+  S PTVYTIDP KITSL+I
Sbjct: 421 SIPFLQPSPASEATLKFLSDAILCLTRKEEICNSPSKVINDSSFPTVYTIDPKKITSLNI 480

Query: 481 ALSETRTTSMPNQIQYAIEFVPTMRDGNKGG----VDLGSESCGKKILCWKFHWTDNEKL 540
           ALSE +T          IE VPTM+ G++GG    V+ GSE C KKIL WKFH  DN KL
Sbjct: 481 ALSEVQT----------IELVPTMKGGDEGGVGSEVESGSEPCAKKILVWKFHVMDNAKL 540

Query: 541 IRALKDLIKLHEPSIVLIFGTKISGADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQD 585
           +RALKDLI+LHEPSIVLIFG KISG D DKV++EL FC SY  KPDGYNGGVWLLLS QD
Sbjct: 541 MRALKDLIQLHEPSIVLIFGNKISGVDTDKVMRELAFCGSYSSKPDGYNGGVWLLLSKQD 600

BLAST of CmaCh04G012490 vs. NCBI nr
Match: KAG6600114.1 (hypothetical protein SDJN03_05347, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 677.2 bits (1746), Expect = 1.3e-190
Identity = 374/635 (58.90%), Postives = 443/635 (69.76%), Query Frame = 0

Query: 4   QSDYQLTGAGDDEAAAR---NRKK--------------------AIVCELSASQTARITQ 63
           QS+ Q TGAGDDEAAAR   +RKK                    A VC LS SQTARITQ
Sbjct: 9   QSNLQPTGAGDDEAAARTYLSRKKAKRPLMASSSDLESHRSTTGATVCNLSPSQTARITQ 68

Query: 64  QFDHSFIAWIFGKDVRPWRIASLLRRHLCLTGTVKVFELGLGYFVLKFCETDFLALQDVP 123
           QFDHS IAW+FG+D+RP ++A  LRRHL LT  V+VFELGLGYFVLKF ETD+LAL+D+P
Sbjct: 69  QFDHSLIAWVFGQDIRPRQLAGRLRRHLHLTQDVEVFELGLGYFVLKFSETDYLALEDLP 128

Query: 124 WSVPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQKIAAAIGGDLVKI 183
           WS+PNLCI+   WTPDFKPSE I SSVDVW+RLHELSIEYYD+E+L++IAA IGG LVK 
Sbjct: 129 WSIPNLCIYAFRWTPDFKPSEAINSSVDVWIRLHELSIEYYDEEILRQIAATIGGVLVKF 188

Query: 184 DPVTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQEIEYEGFELLCPNCSRVDGLRHNC 243
           DPVTKNR KCKFARIC+R+NLCDPLPSMI+LG+I+Q+IEYEG +LLCPNC  V  L+ NC
Sbjct: 189 DPVTKNRRKCKFARICIRINLCDPLPSMIKLGRIQQKIEYEGLDLLCPNCRLVHDLKQNC 248

Query: 244 LNLKIPSGFSGFNPHRDKP-HHHGAR---------SFKQPLIPSESSAPSPRGSRFQVLD 303
           LN   PSG SG +   D+P HHH  R         S KQPLIPS SS  S  GSRFQVL+
Sbjct: 249 LNSGNPSGSSGLDTLGDRPTHHHRTRPLLELGSSSSSKQPLIPSVSSPASACGSRFQVLE 308

Query: 304 LNSNEWPSLGESGKAGTSIRTSSSPVHVKDKAIAKKKEKCGDSVQPLP----KESSRVT- 363
            +      L E  KA  SIR SS  VHVKDKA AK KE CGD V+ LP    K S++ T 
Sbjct: 309 NDL----LLDECEKASPSIRISSPHVHVKDKAAAKPKELCGDPVRSLPKLPKKPSTKTTK 368

Query: 364 -----------IKDQLKAAKTSNNPTVNEQPPSPTPCILPLQPCPASEAVLEFHSAAIQR 423
                      ++ Q K AKTS NPT+     +        QPC   +A L+F SA I+R
Sbjct: 369 APELELVAPAVVEHQFKPAKTS-NPTLIADHNN--------QPCLVPKATLDFISAVIRR 428

Query: 424 STRTKEITSVPSKEISVDSRPTVYTID-PKITSLDIALSETRTTSMPNQIQYAIEFVPTM 483
           S + KE+  +PSKEI VD  P V+TI+  KI S  + LS  +T S+PN+  Y ++ +PT 
Sbjct: 429 SMKEKEMPDIPSKEIIVDGCPIVHTINTKKIRSFKMNLSALQTNSIPNRNHYTMDTLPTA 488

Query: 484 R----DGNKGGVDLGSESCGKKILCWKFHWTDNEKLIRALKDLIKLHEPSIVLIFGTKIS 543
           R    DG+      GSESC KK+LCWKFH TDN  L++ALKDLI+LHEPSIVLIFGTKIS
Sbjct: 489 RCVDEDGDGSKTVSGSESCSKKMLCWKFHGTDNANLMQALKDLIQLHEPSIVLIFGTKIS 548

Query: 544 GADVDKVVQELPFCYSYYRKPDGYNGGVWLLLSNQDVETKVNSCSPQQVCASIYFDSKTT 585
           GA+ + VV+EL FC SY RKPDGYNGGVWLLLS QDV+ +V+S SPQQV AS+YF S T 
Sbjct: 549 GAEAEHVVRELSFCGSYCRKPDGYNGGVWLLLSRQDVQIEVDSYSPQQVSASVYFGSNTN 608

BLAST of CmaCh04G012490 vs. TAIR 10
Match: AT2G01050.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 90.9 bits (224), Expect = 3.7e-18
Identity = 50/152 (32.89%), Postives = 78/152 (51.32%), Query Frame = 0

Query: 71  TGTVKVFELGLGYFVLKF-CETDFL-ALQDVPWSVPNLCIHVSPWTPDFKPSETILSSVD 130
           +G + V +L   +F+++F  E +++ AL   PW V    + V  W+  F P    + +  
Sbjct: 106 SGVMTVMDLPRQFFMIRFELEEEYMAALTGGPWRVLGNYLLVQDWSSRFDPLRDDIVTTP 165

Query: 131 VWVRLHELSIEYYDDEVLQKIAAAIGGDLVKIDPVTKNRLKCKFARICVRVNLCDPLPSM 190
           VWVRL  +   YY   +L +IA  +G  L K+D  T N  K +FAR+C+ VNL  PL   
Sbjct: 166 VWVRLSNIPYNYYHRCLLMEIARGLGRPL-KVDMNTINFDKGRFARVCIEVNLAKPLKGT 225

Query: 191 IRLGKIRQEIEYEGFELLCPNCSRVDGLRHNC 221
           + +   R  + YEG   +C +C     L H+C
Sbjct: 226 VLINGDRYFVAYEGLSKICSSCGIYGHLVHSC 256

BLAST of CmaCh04G012490 vs. TAIR 10
Match: AT5G36228.1 (nucleic acid binding;zinc ion binding )

HSP 1 Score: 62.8 bits (151), Expect = 1.1e-09
Identity = 40/137 (29.20%), Postives = 68/137 (49.64%), Query Frame = 0

Query: 90  ETDFL-ALQDVPWSVPNLCIHVSPWTPDFKPSETILSSVDVWVRLHELSIEYYDDEVLQK 149
           E D L  L+  PW      I +  W  DF P+E  L+ +DVWV +  + + Y  +  ++ 
Sbjct: 85  EIDLLNGLRRAPWVFNEWFIALQRW-EDF-PTEDFLTFIDVWVHIRGIPLPYVSERTVEI 144

Query: 150 IAAAIGGDLVKIDPVTKNRLKCKFARICVRVNLCDPLPSMIRLGKIRQE-----IEYEGF 209
           IA+ + G++V +D   +   +  F R+ VR++  +PL    R+    +E      EYE  
Sbjct: 145 IASTL-GEVVAMDFNEETTSQITFIRVKVRMDFTEPLRFFRRVRFASRERAMIGFEYEKL 204

Query: 210 ELLCPNCSRVDGLRHNC 221
           + +C NC RV+    +C
Sbjct: 205 QRVCTNCCRVNHQVSHC 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7SSJ36.2e-20762.46DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A0A0KLB02.1e-19158.88DUF4283 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175790 PE=... [more]
A0A6J1FN131.7e-13549.50uncharacterized protein LOC111446932 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1FU802.8e-13549.34uncharacterized protein LOC111446932 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7SUD33.1e-12143.19DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
Match NameE-valueIdentityDescription
KAG7031864.10.0e+0094.71hypothetical protein SDJN02_05905, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6601052.10.0e+0094.54hypothetical protein SDJN03_06285, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAA0034062.11.3e-20662.46uncharacterized protein E6C27_scaffold65G00480 [Cucumis melo var. makuwa][more]
KGN50455.14.4e-19158.88hypothetical protein Csa_000357 [Cucumis sativus][more]
KAG6600114.11.3e-19058.90hypothetical protein SDJN03_05347, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT2G01050.13.7e-1832.89zinc ion binding;nucleic acid binding [more]
AT5G36228.11.1e-0929.20nucleic acid binding;zinc ion binding [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025558Domain of unknown function DUF4283PFAMPF14111DUF4283coord: 37..177
e-value: 2.2E-21
score: 75.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 277..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..265
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 277..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..265
NoneNo IPR availablePANTHERPTHR31286:SF74BNAA05G15600D PROTEINcoord: 15..473
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 15..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G012490.1CmaCh04G012490.1mRNA