Homology
BLAST of CmaCh04G011570 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 819.3 bits (2115), Expect = 3.5e-236
Identity = 425/736 (57.74%), Postives = 528/736 (71.74%), Query Frame = 0
Query: 3 SLALRLVFLNLLFT-LLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGSA 62
SL +L F +L F+ L + S+ DG+ IYIVYMG K +D AHLHHRAMLE+VVGS
Sbjct: 4 SLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGST 63
Query: 63 FSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTRD 122
F+ ES++++YKRSFNGFAVKLTEEEA+++A EGVVSVF ++ N+L TTRSWDF+GF
Sbjct: 64 FAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLT 123
Query: 123 VPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGARA 182
VPR Q ES+I+VGV DTGIWP+S SF D+G PPPPKWKGTCE S NFRCNRKIIGAR+
Sbjct: 124 VPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARS 183
Query: 183 YRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVYK 242
Y P+ P + GP DTNGHGTH AST AG LV A+LYG GLG ARGGVP ARIA YK
Sbjct: 184 YHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYK 243
Query: 243 VCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL-VRPFFHDGIAIGAYQAMRNGILTSN 302
VCW C D D+LAA+DDAIADGVDI+SLS+GG R +F D IAIG++ A+ GILTSN
Sbjct: 244 VCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSN 303
Query: 303 SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL 362
SAGN GPN++TT SL+PW+LSVAA+T+DR F VQ+GNG +QGV+IN FD + YPL
Sbjct: 304 SAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPL 363
Query: 363 VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGAS---FPSLNGAVGVVME 422
V D+PN GF+ + SRFC SV+ NL++GKIV+C++S F SL+GA GV+M
Sbjct: 364 VSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMT 423
Query: 423 GTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSR 482
T ++PLP+S +P YI S +P ATIF+S + + +APV FSSR
Sbjct: 424 SNTR-DYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 483
Query: 483 GPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAY 542
GPN + +++KPD+S PGV ILAAWP P+ G ++ +N SGTSM+CPH+T IA Y
Sbjct: 484 GPNRATKDVIKPDISGPGVEILAAWPSVAPVG-GIRRNTLFNIISGTSMSCPHITGIATY 543
Query: 543 VKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDY 602
VKT+NP WSPAAIKSALMTTA PM+ + N QAEF+YGSG +NPLKAV PGLVY+A+ESDY
Sbjct: 544 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 603
Query: 603 VEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLT 662
V++LCG G +R ITG+ CT NTGR DLNYPSF L + Q+ +F RTLT
Sbjct: 604 VKFLCG-QGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLT 663
Query: 663 NVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSAS 722
+V STYRA + +P GL + V+P L+F+G+GD KSFTL V+G + VSAS
Sbjct: 664 SVAPQASTYRAMISAPQGLTI--SVNPNVLSFNGLGDRKSFTLTVRGSIKG---FVVSAS 723
Query: 723 LVWSNGVREVRSPIVL 734
LVWS+GV VRSPI +
Sbjct: 724 LVWSDGVHYVRSPITI 727
BLAST of CmaCh04G011570 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 608.6 bits (1568), Expect = 9.3e-173
Identity = 338/743 (45.49%), Postives = 457/743 (61.51%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRK---IYIVYMGSKPKDSSQAHLHHRAMLEEV 60
M L+ L + L F + T S D R+ +YIVYMG+ P+ HH ++L+++
Sbjct: 1 MAKLSTPLYLICLAF--IFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKL 60
Query: 61 VGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIG 120
VG+ ++ +V SYKRSFNGFA L++ E+Q++ + + VVSVFPSK+++L TTRSWDF+G
Sbjct: 61 VGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVG 120
Query: 121 FTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKII 180
F R +ESD+IVGV D+GIWP+S SF D+G GPPP KWKG+C+ F CN K+I
Sbjct: 121 FGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLI 180
Query: 181 GARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARI 240
GAR Y A D GHGTH AST AG V AS YG G ARGGVPSARI
Sbjct: 181 GARFYNKF-------ADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARI 240
Query: 241 AVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGG-LVRPFFHDGIAIGAYQAMRNGI 300
A YKVC+ R C D D+LAAFDDAIADGVD++S+SI V + +AIG++ AM GI
Sbjct: 241 AAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGI 300
Query: 301 LTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGK 360
+T+ SAGN+GP+ + +++PWM++VAA+ DR F V LGNG G+++N F+L G
Sbjct: 301 ITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGT 360
Query: 361 QYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDS-SGASFPSLNGAVGVV 420
++P+VY N+ + + +C VD LV+GKIVLCD G L GA+GV+
Sbjct: 361 KFPIVYG---QNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVI 420
Query: 421 MEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFS 480
++ T P PAS I+ YI S P A I R+ E+ AP FS
Sbjct: 421 VQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFS 480
Query: 481 SRGPNAISPNILKPDLSAPGVNILAAW-PQNVP---ISPGDPLSSPYNFASGTSMACPHV 540
SRGP+ + N+LKPD+SAPG+ ILAA+ P P ++P D S Y+ SGTSMACPHV
Sbjct: 481 SRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHV 540
Query: 541 TAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYN 600
+AAYVK+F+P WSP+AIKSA+MTTA PM++K N + EF+YGSGQINP KA +PGLVY
Sbjct: 541 AGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYE 600
Query: 601 ASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVF 660
DY++ LC G L +G+++ C++ + DLNYP+ F+ V
Sbjct: 601 VETEDYLKMLCA-EGFDSTTLTTTSGQNVTCSERTEVK--DLNYPTMTTFVSSLDPFNVT 660
Query: 661 FPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP 720
F RT+TNV STY+A+V P +Q ++P L F + + KSF + + G D
Sbjct: 661 FKRTVTNVGFPNSTYKASVV-PLQPELQISIEPEILRFGFLEEKKSFVVTISG-KELKDG 720
Query: 721 TTVSASLVWSNGVREVRSPIVLF 735
+ VS+S+VWS+G VRSPIV +
Sbjct: 721 SFVSSSVVWSDGSHSVRSPIVAY 725
BLAST of CmaCh04G011570 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 584.7 bits (1506), Expect = 1.4e-165
Identity = 332/739 (44.93%), Postives = 454/739 (61.43%), Query Frame = 0
Query: 9 VFLNLLFTLLITTSASNGD--GRKIYIVYMGSKPKDSSQAHL-HHRAMLEEVVGSAFSAE 68
+F +LL L + SA D +++YIVY+GS P + H ++L+E+ G +
Sbjct: 10 LFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIEN 69
Query: 69 SIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTRDV--P 128
+V SYK+SFNGFA +LTE E +R+A E VVSVFPS+ KLQTT SW+F+G +
Sbjct: 70 RLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTK 129
Query: 129 RIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGARAYR 188
R ESD I+GV D+GI+P+S SF DQG GPPP KWKGTC NF CN K+IGAR Y
Sbjct: 130 RTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYT 189
Query: 189 AVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVYKVC 248
A T D +GHGTH AS AG V N++ YG G G ARGGVP+ARIAVYKVC
Sbjct: 190 AKSKANQTAR----DYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC 249
Query: 249 WGRTCEDADMLAAFDDAIADGVDILSLSIG-GLVRPFFHDGIAIGAYQAMRNGILTSNSA 308
C+ M++AFDDAIADGVD++S+SI + PF D IAIGA+ AM G+LT N+A
Sbjct: 250 DNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAA 309
Query: 309 GNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVY 368
GN+GP T S APW+ SVAA+ +R F A V LG+G + G ++N +D+ G YPLVY
Sbjct: 310 GNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVY 369
Query: 369 AGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSS-GASFPSLNGAVG-VVMEGTT 428
+ + +R C +D LV+GKIVLCDS+ G GAVG +V
Sbjct: 370 GKSAAL--STCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEP 429
Query: 429 TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN 488
IR+FP+ S + + Y+ S P AT+ +S E+++ AP+ A FSSRGP+
Sbjct: 430 DRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPS 489
Query: 489 AISPNILKPDLSAPGVNILAAW-PQNVPI-SPGDPLSSPYNFASGTSMACPHVTAIAAYV 548
+I +ILKPD++APGV ILAA+ P + P S D Y+ SGTSMACPHV +AAYV
Sbjct: 490 SIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYV 549
Query: 549 KTFNPWWSPAAIKSALMTTAFPMDVKVNR--QAEFSYGSGQINPLKAVNPGLVYNASESD 608
KTF+P WSP+ I+SA+MTTA+PM+ + EF+YGSG ++P+ A+NPGLVY +++D
Sbjct: 550 KTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKAD 609
Query: 609 YVEYLCGIPGITPAQLRLITGEDIICT-DSNTGRDLDLNYPSFALFMGRGQSLRVFFPRT 668
++ +LCG+ T LR+I+G++ CT + + +LNYP+ + + + + F RT
Sbjct: 610 HINFLCGL-NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRT 669
Query: 669 LTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVS 728
+TNV STY A V PG + KV P L+ + + +SF + V T VS
Sbjct: 670 VTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT-KQPVS 729
Query: 729 ASLVWSNGVREVRSPIVLF 735
A+L+WS+G VRSPI+++
Sbjct: 730 ANLIWSDGTHNVRSPIIVY 738
BLAST of CmaCh04G011570 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 582.8 bits (1501), Expect = 5.5e-165
Identity = 322/742 (43.40%), Postives = 447/742 (60.24%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHL-HHRAMLEEVVG 60
M +LA L+ L L +++ ++ D +++YIVYMGS + H +L+EV G
Sbjct: 1 MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60
Query: 61 SAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFT 120
+ +V SYKRSFNGFA +LTE E +R+A GVVSVFP+K +LQTT SWDF+G
Sbjct: 61 ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120
Query: 121 RDV--PRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKII 180
+ R P ESD I+GV D+GI P+S SF D+G GPPP KWKG C NF CN K+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180
Query: 181 GARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARI 240
GAR Y + G D +GHGTH AST AG V +AS +G G G RGGVP++R+
Sbjct: 181 GARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 240
Query: 241 AVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFF-HDGIAIGAYQAMRNGI 300
A YKVC C +L+AFDDAIADGVD++++SIG F +D IAIGA+ AM G+
Sbjct: 241 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 300
Query: 301 LTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGK 360
LT NSAGN GP + +APW+L+VAA+T +R F V LGNG G ++N +++ GK
Sbjct: 301 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 360
Query: 361 QYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDS-SGASFPSLNGAVGVV 420
YPLVY + ++ + C ++ VD++ V+GKI++C G GAVG++
Sbjct: 361 DYPLVYGKSAAS--SACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLI 420
Query: 421 MEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFS 480
T + PLPA+ + Y+ S +P A + ++ + + +PV A FS
Sbjct: 421 YR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 480
Query: 481 SRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIA 540
SRGPN I+ +ILKPD++APGV ILAA+ S D Y+ SGTSM+CPHV +A
Sbjct: 481 SRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVA 540
Query: 541 AYVKTFNPWWSPAAIKSALMTTAFPMDVKVN--RQAEFSYGSGQINPLKAVNPGLVYNAS 600
AYVKTFNP WSP+ I+SA+MTTA+P++ EF+YGSG ++P+ A NPGLVY
Sbjct: 541 AYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELD 600
Query: 601 ESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSF-ALFMGRGQSLRVFF 660
+SD++ +LCG+ T L++I+GE + C+++ +LNYPS A G G + V F
Sbjct: 601 KSDHIAFLCGM-NYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 660
Query: 661 PRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPT 720
RTLTNV STY + V + G + K+ P L+F + + +SFT+ V G +
Sbjct: 661 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTG-SNLDSEV 720
Query: 721 TVSASLVWSNGVREVRSPIVLF 735
SA+L+WS+G VRSPIV++
Sbjct: 721 PSSANLIWSDGTHNVRSPIVVY 728
BLAST of CmaCh04G011570 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 574.3 bits (1479), Expect = 1.9e-162
Identity = 328/746 (43.97%), Postives = 446/746 (59.79%), Query Frame = 0
Query: 5 ALRLVFLNLLFTLLITTSASNG---DGRKIYIVYMGSKPKDSSQAHL-HHRAMLEEVVGS 64
A+ L+ +F LL+ + AS G +++YIVYMG+ P + HH ++L++V G
Sbjct: 4 AVSYCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGE 63
Query: 65 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFT- 124
+ + +V +YKRSFNGFA +LTE E + +A + VVSVFPSKN LQTT SW+F+G
Sbjct: 64 SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 123
Query: 125 -RDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIG 184
+ R P ESD I+GV D+GI+P+S SF +G GPPP KWKG C+ NF CN K+IG
Sbjct: 124 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 183
Query: 185 ARAYRAVGPLPPTEAW--GPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSAR 244
AR Y P E + D GHG+H AS AG V + S YG G G RGGVP+AR
Sbjct: 184 ARYY-----TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAAR 243
Query: 245 IAVYKVCWGRT--CEDADMLAAFDDAIADGVDILSLSIGG-LVRPFFHDGIAIGAYQAMR 304
IAVYKVC C +LAAFDDAIAD VDI+++S+G V F D +AIGA+ AM
Sbjct: 244 IAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMA 303
Query: 305 NGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDL 364
GILT N AGN+GP T S+APW+ +VAA+ ++R F V LGNG G ++N FDL
Sbjct: 304 KGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDL 363
Query: 365 GGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSS-GASFPSLNGAV 424
GK+YPLVY + +++ + FC +D V+GKIVLCD+ GAV
Sbjct: 364 NGKKYPLVYG---KSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAV 423
Query: 425 GVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAA 484
++ F P S + N + Y+ S P A + +S + + APV A
Sbjct: 424 ASIVRNPYE-DAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 483
Query: 485 DFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVT 544
+SSRGPN + +ILKPD++APG ILAA+ VP S D Y SGTSM+CPHV
Sbjct: 484 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSESDTRHVKYTVISGTSMSCPHVA 543
Query: 545 AIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKV---NRQAEFSYGSGQINPLKAVNPGLV 604
+AAY+KTF+P WSP+ I+SA+MTTA+PM+ N AEF+YG+G ++P+ A++PGLV
Sbjct: 544 GVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLV 603
Query: 605 YNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNT-GRDLDLNYPSFALFMGRGQSL 664
Y A++SD++ +LCG T +LRLI+G+ CT T +LNYPS + + +
Sbjct: 604 YEANKSDHITFLCGF-NYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPF 663
Query: 665 RVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSA 724
+V F RT+TNV +TY+A V G ++ KV P L+ + + KSFT+ V G
Sbjct: 664 KVTFRRTVTNVGRPNATYKAKVV---GSKLKVKVVPAVLSLKSLYEKKSFTVTVSG-AGP 723
Query: 725 TDPTTVSASLVWSNGVREVRSPIVLF 735
VSA L+WS+GV VRSPIV++
Sbjct: 724 KAENLVSAQLIWSDGVHFVRSPIVVY 735
BLAST of CmaCh04G011570 vs. ExPASy TrEMBL
Match:
A0A6J1IMT3 (cucumisin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1)
HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS
Sbjct: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
Query: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR
Sbjct: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
Query: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSN 300
KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSN
Sbjct: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSN 300
Query: 301 SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL 360
SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL
Sbjct: 301 SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL 360
Query: 361 VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTT 420
VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTT
Sbjct: 361 VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTT 420
Query: 421 TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN 480
TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN
Sbjct: 421 TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN 480
Query: 481 AISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKT 540
AISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKT
Sbjct: 481 AISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKT 540
Query: 541 FNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEY 600
FNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEY
Sbjct: 541 FNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEY 600
Query: 601 LCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVE 660
LCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVE
Sbjct: 601 LCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVE 660
Query: 661 ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW 720
ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW
Sbjct: 661 ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW 720
Query: 721 SNGVREVRSPIVLF 735
SNGVREVRSPIVLF
Sbjct: 721 SNGVREVRSPIVLF 734
BLAST of CmaCh04G011570 vs. ExPASy TrEMBL
Match:
A0A6J1IP33 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1)
HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 699/699 (100.00%), Postives = 699/699 (100.00%), Query Frame = 0
Query: 36 MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG 95
MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG
Sbjct: 1 MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG 60
Query: 96 VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP 155
VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP
Sbjct: 61 VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP 120
Query: 156 PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV 215
PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV
Sbjct: 121 PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV 180
Query: 216 DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL 275
DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL
Sbjct: 181 DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL 240
Query: 276 VRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHV 335
VRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHV
Sbjct: 241 VRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHV 300
Query: 336 QLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKI 395
QLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKI
Sbjct: 301 QLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKI 360
Query: 396 VLCDSSGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTA 455
VLCDSSGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTA
Sbjct: 361 VLCDSSGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTA 420
Query: 456 TIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLS 515
TIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLS
Sbjct: 421 TIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLS 480
Query: 516 SPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGS 575
SPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGS
Sbjct: 481 SPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGS 540
Query: 576 GQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNY 635
GQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNY
Sbjct: 541 GQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNY 600
Query: 636 PSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDT 695
PSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDT
Sbjct: 601 PSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDT 660
Query: 696 KSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 735
KSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF
Sbjct: 661 KSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 699
BLAST of CmaCh04G011570 vs. ExPASy TrEMBL
Match:
A0A6J1GX71 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458261 PE=3 SV=1)
HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 670/735 (91.16%), Postives = 691/735 (94.01%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
MC LALR+VFLNLL T LI+ SASNGDGRKIYIVYMGSKPKDSSQ HLHHRAMLEEVVGS
Sbjct: 1 MCCLALRVVFLNLLCTSLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMA KEGVVSVFPSKN KLQTTRSWDFIGF R
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYKLQTTRSWDFIGFPR 120
Query: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
DVPR+PQQESDIIVGV DTGIWPDSLSFHDQGLG PPPKWKG CEASANFRCNRKIIGAR
Sbjct: 121 DVPRVPQQESDIIVGVLDTGIWPDSLSFHDQGLGAPPPKWKGICEASANFRCNRKIIGAR 180
Query: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRA GP PPT+AWGPIDT+GHGTHVASTVAG LVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGG-LVRPFFHDGIAIGAYQAMRNGILTS 300
KVCW R C DA++LAAFDDAIADGVDILS+SIGG VRPFF +GIAIGA+QAMRNGIL S
Sbjct: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
Query: 301 NSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGNDGPNWYTTGS+APWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP
Sbjct: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
Query: 361 LVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGT 420
LVYAGDVPNIGGGF+S+ISRFC VNSVDRNLVRGKIVLCDSS SFPSLNGAVGVVME T
Sbjct: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
Query: 421 TTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGP 480
+W LI TFPLPASHFNPPSANPIRLYITSNPAPTATIFRSV VN TNAPVAA FSSRGP
Sbjct: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
Query: 481 NAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVK 540
N ISPNILKPDLSAPGVNILAAWPQNVPIS G PLSSPYNFASGTSMACPH TAIAAYVK
Sbjct: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
Query: 541 TFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVE 600
TF+PWWSPAAIKSALMTTA PMD + ++ AEF+YGSGQINPLKAVNPGLVYNASESDYVE
Sbjct: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
Query: 601 YLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNV 660
YLCGIP IT AQL+ ITGEDIICTDSNTGRDLDLNYPSFAL MGRGQSLRVFFPRTLTNV
Sbjct: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
Query: 661 EATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLV 720
EATVSTYRATVYSPPGLN+QFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP TVSASLV
Sbjct: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
Query: 721 WSNGVREVRSPIVLF 735
WSNGVREVRSPIVLF
Sbjct: 721 WSNGVREVRSPIVLF 735
BLAST of CmaCh04G011570 vs. ExPASy TrEMBL
Match:
A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)
HSP 1 Score: 887.1 bits (2291), Expect = 5.0e-254
Identity = 451/743 (60.70%), Postives = 552/743 (74.29%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
M SL + +F +L + LL++ S + DGRK YIVYMGSKP+D++ LHHRAMLEEV+GS
Sbjct: 7 MSSLIFKFIFFSLFYRLLVSGSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAMLEEVIGS 66
Query: 61 AF---SAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIG 120
F + ES++YSYKRSFNGFAV+LTEEEA ++A KEGVVSVFP+ L TTRSWDF+G
Sbjct: 67 TFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMG 126
Query: 121 FTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKII 180
F++ VPR Q ESD++VGV DTGIWP+S SF D+ GPPP KW G C+A+ +FRCN+KII
Sbjct: 127 FSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKII 186
Query: 181 GARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARI 240
GAR YR+ LPP++ P D+NGHGTH ASTVAG LV+ ASLYG G+G ARGGVPSARI
Sbjct: 187 GARVYRSES-LPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSARI 246
Query: 241 AVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL-VRPFFHDGIAIGAYQAMRNGI 300
AVYK+CW C DAD+LAAFDDAIADGVDI+SLS+GG +F D IAIGA+ AM++GI
Sbjct: 247 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAFHAMKHGI 306
Query: 301 LTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGK 360
LTSNSAGN+GPN++T +++PW LSVAA+T DR VQLGNGN+YQGVAIN FDL GK
Sbjct: 307 LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQGVAINTFDLLGK 366
Query: 361 QYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDS--SGASFPSLNGAVGV 420
QYPL+YAGD PNI GGFN +ISR C NSVD+NLVRGKI++CDS + S N VG+
Sbjct: 367 QYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGI 426
Query: 421 VMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADF 480
VM+ ++P PAS+ + + I+LYI+S PTATI +S+ V +APV F
Sbjct: 427 VMQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSF 486
Query: 481 SSRGPNAISPNILKPDLSAPGVNILAAWPQNVPI--SPGDPLSSPYNFASGTSMACPHVT 540
SSRGP I+ +ILK DLSAPGV ILAAW P+ GD S YN SGTSM+CPH T
Sbjct: 487 SSRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHAT 546
Query: 541 AIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNA 600
AIAAYVKTF+P WSPAA+KSALMTTAF M+ K+N QAEF+YGSG +NPLKAVNPGLVY+A
Sbjct: 547 AIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDA 606
Query: 601 SESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFF 660
+E DYV++LCG G + A +R ITG+ CT N GR DLNYPSFAL + + FF
Sbjct: 607 NEGDYVKFLCG-QGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF 666
Query: 661 PRTLTNVEATVSTYRATVYSPP-GLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP 720
RTLTNVE+ STY A V+ PP GL++ V+P L+F+G+GDTKSF L VQG +S
Sbjct: 667 RRTLTNVESGASTYSAVVFGPPDGLHI--TVNPSVLSFNGVGDTKSFNLTVQGTVS---Q 726
Query: 721 TTVSASLVWSNGVREVRSPIVLF 735
VSASL WS+GV +VRSPI ++
Sbjct: 727 AIVSASLEWSDGVHQVRSPITIY 742
BLAST of CmaCh04G011570 vs. ExPASy TrEMBL
Match:
K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)
HSP 1 Score: 882.5 bits (2279), Expect = 1.2e-252
Identity = 454/738 (61.52%), Postives = 562/738 (76.15%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
M SL +L+FL+L +LL+++S SN DGRKIYIVYMGSK +D++ AHL+HRAMLEEVVGS
Sbjct: 6 MSSLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGS 65
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
F+ ES++Y+YKRSFNGFAVKLTEEEA ++A KEGVVSVFPS+ N L TTRSWDF+G ++
Sbjct: 66 TFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQ 125
Query: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
+VPR+ Q ES+I+VGVFD+GIWP++ SF+D G GP P W+GTC+AS NFRCNRKIIGAR
Sbjct: 126 NVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGAR 185
Query: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
AYR+ LPP + P DT+GHGTH ASTVAG LV ASLYG G+G ARGGVP ARIAVY
Sbjct: 186 AYRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVY 245
Query: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLV-RPFFHDGIAIGAYQAMRNGILTS 300
K+CW C DAD+LAAFDDAIADGVDI+SLS+GG V +P+ ++ IAIG++ AM+ GILTS
Sbjct: 246 KICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTS 305
Query: 301 NSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGN+GP +T SL+PW+ +VAA++ DR F V LGNGN YQGV+IN FD+ QYP
Sbjct: 306 NSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDM-RNQYP 365
Query: 361 LVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSS--GASFPSLNGAVGVVME 420
L+YAG+ P+I GFNS+ SR+C +SVD NLVRGKI+LCDS+ F S GA GV+M+
Sbjct: 366 LIYAGNAPSI--GFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLMQ 425
Query: 421 GTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSR 480
T ++PLPAS +P N I+ Y++S APTATIF+S V T+APV FSSR
Sbjct: 426 SNTR-DHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSR 485
Query: 481 GPNAISPNILKPDLSAPGVNILAAWPQNVPIS-PGDPLSSPYNFASGTSMACPHVTAIAA 540
GPN ++ +ILKPD +APGV ILAAWP PIS D S+ YN SGTSM+CPHVTAIA
Sbjct: 486 GPNYVTHDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTAIAV 545
Query: 541 YVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESD 600
++KTF P WSPAAIKSALMTTA PM+ + N AEF+YGSG +NPLKAV+PGLVY+ASESD
Sbjct: 546 HIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESD 605
Query: 601 YVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTL 660
YV++LCG G T A +R TG++ CT N GR DLNYPSFAL + R Q+ F RTL
Sbjct: 606 YVKFLCG-EGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTANQSFRRTL 665
Query: 661 TNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSA 720
TNV + STYRA++ +P GL++ V+P L+F+GIGD KSFTL V+G +S VSA
Sbjct: 666 TNVVSGASTYRASISAPQGLSI--SVNPSVLSFNGIGDQKSFTLTVRGTVS---QAIVSA 725
Query: 721 SLVWSNGVREVRSPIVLF 735
SLVWS+G VRSPI ++
Sbjct: 726 SLVWSDGSHNVRSPITVY 732
BLAST of CmaCh04G011570 vs. NCBI nr
Match:
XP_022976643.1 (cucumisin-like isoform X2 [Cucurbita maxima])
HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS
Sbjct: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
Query: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR
Sbjct: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
Query: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSN 300
KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSN
Sbjct: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSN 300
Query: 301 SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL 360
SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL
Sbjct: 301 SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL 360
Query: 361 VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTT 420
VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTT
Sbjct: 361 VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTT 420
Query: 421 TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN 480
TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN
Sbjct: 421 TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN 480
Query: 481 AISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKT 540
AISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKT
Sbjct: 481 AISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKT 540
Query: 541 FNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEY 600
FNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEY
Sbjct: 541 FNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEY 600
Query: 601 LCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVE 660
LCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVE
Sbjct: 601 LCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVE 660
Query: 661 ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW 720
ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW
Sbjct: 661 ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW 720
Query: 721 SNGVREVRSPIVLF 735
SNGVREVRSPIVLF
Sbjct: 721 SNGVREVRSPIVLF 734
BLAST of CmaCh04G011570 vs. NCBI nr
Match:
XP_022976634.1 (cucumisin-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 699/699 (100.00%), Postives = 699/699 (100.00%), Query Frame = 0
Query: 36 MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG 95
MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG
Sbjct: 1 MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG 60
Query: 96 VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP 155
VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP
Sbjct: 61 VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP 120
Query: 156 PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV 215
PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV
Sbjct: 121 PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV 180
Query: 216 DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL 275
DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL
Sbjct: 181 DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGL 240
Query: 276 VRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHV 335
VRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHV
Sbjct: 241 VRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHV 300
Query: 336 QLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKI 395
QLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKI
Sbjct: 301 QLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKI 360
Query: 396 VLCDSSGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTA 455
VLCDSSGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTA
Sbjct: 361 VLCDSSGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTA 420
Query: 456 TIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLS 515
TIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLS
Sbjct: 421 TIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLS 480
Query: 516 SPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGS 575
SPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGS
Sbjct: 481 SPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGS 540
Query: 576 GQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNY 635
GQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNY
Sbjct: 541 GQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNY 600
Query: 636 PSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDT 695
PSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDT
Sbjct: 601 PSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDT 660
Query: 696 KSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 735
KSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF
Sbjct: 661 KSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 699
BLAST of CmaCh04G011570 vs. NCBI nr
Match:
XP_023548098.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 688/734 (93.73%), Postives = 703/734 (95.78%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
MC LALR+VFLNLLFTLLITTSASNGDGRKIYIVYMGSKP DSSQ HLHHRAMLEEVVGS
Sbjct: 14 MCCLALRVVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPTDSSQPHLHHRAMLEEVVGS 73
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
AFSAESIVYSYKRSFNGFAVKLT+EEAQRMADKEGVVSVFPSKN KLQTTRSWDFIGF
Sbjct: 74 AFSAESIVYSYKRSFNGFAVKLTDEEAQRMADKEGVVSVFPSKNYKLQTTRSWDFIGFPP 133
Query: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
DVPR+PQQESDIIVGV DTGIWPDSLSFHDQGLG PPPKWKGTCEASANFRCNRKIIGAR
Sbjct: 134 DVPRVPQQESDIIVGVLDTGIWPDSLSFHDQGLGAPPPKWKGTCEASANFRCNRKIIGAR 193
Query: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRA GPLPPT+AWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 194 AYRAEGPLPPTQAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 253
Query: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSN 300
KVCW R C DA++LAAFDDAIADGVDILSLSIG LVRPFF DGIAIGAYQAMRNGILTSN
Sbjct: 254 KVCWERGCSDANILAAFDDAIADGVDILSLSIGDLVRPFFEDGIAIGAYQAMRNGILTSN 313
Query: 301 SAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPL 360
AGN G NWYTTGS+APWMLSVAANTIDRNF +HVQLGNGNVYQGVAINLFDLGGKQYPL
Sbjct: 314 GAGNGGTNWYTTGSMAPWMLSVAANTIDRNFVSHVQLGNGNVYQGVAINLFDLGGKQYPL 373
Query: 361 VYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTT 420
VYAGDVPNI GGF+S+ SRFCGVNSVDRNLVRGKIVLCDSS SFPSLNGAVGVVME TT
Sbjct: 374 VYAGDVPNIAGGFSSSTSRFCGVNSVDRNLVRGKIVLCDSSAVSFPSLNGAVGVVMESTT 433
Query: 421 TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN 480
TW LIRTFPLPASHFNPPS NPIRLYITSNPAPTATIFRSV+VNHTNAPVAADFSSRGPN
Sbjct: 434 TWHLIRTFPLPASHFNPPSGNPIRLYITSNPAPTATIFRSVDVNHTNAPVAADFSSRGPN 493
Query: 481 AISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKT 540
AISPNILKPDLSAPGVNILAAWPQNVPISPG+PL SPYNFASGTSMACPH TAIAAYVKT
Sbjct: 494 AISPNILKPDLSAPGVNILAAWPQNVPISPGNPLLSPYNFASGTSMACPHATAIAAYVKT 553
Query: 541 FNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEY 600
F+PWWSPAAIKSALMTTA PMD KVN QAEFS+GSGQINPLKAVNPGLVYNASESDYVEY
Sbjct: 554 FSPWWSPAAIKSALMTTASPMDAKVNTQAEFSHGSGQINPLKAVNPGLVYNASESDYVEY 613
Query: 601 LCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVE 660
LCGIPGIT AQLRLITGEDIICTDSNTGRD+DLNYPSFALFMGRGQSLRVFFPRTLTNVE
Sbjct: 614 LCGIPGITRAQLRLITGEDIICTDSNTGRDVDLNYPSFALFMGRGQSLRVFFPRTLTNVE 673
Query: 661 ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW 720
ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW
Sbjct: 674 ATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVW 733
Query: 721 SNGVREVRSPIVLF 735
SNGVREVRSPIVLF
Sbjct: 734 SNGVREVRSPIVLF 747
BLAST of CmaCh04G011570 vs. NCBI nr
Match:
XP_022956553.1 (cucumisin-like [Cucurbita moschata])
HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 670/735 (91.16%), Postives = 691/735 (94.01%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
MC LALR+VFLNLL T LI+ SASNGDGRKIYIVYMGSKPKDSSQ HLHHRAMLEEVVGS
Sbjct: 1 MCCLALRVVFLNLLCTSLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMA KEGVVSVFPSKN KLQTTRSWDFIGF R
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYKLQTTRSWDFIGFPR 120
Query: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
DVPR+PQQESDIIVGV DTGIWPDSLSFHDQGLG PPPKWKG CEASANFRCNRKIIGAR
Sbjct: 121 DVPRVPQQESDIIVGVLDTGIWPDSLSFHDQGLGAPPPKWKGICEASANFRCNRKIIGAR 180
Query: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRA GP PPT+AWGPIDT+GHGTHVASTVAG LVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGG-LVRPFFHDGIAIGAYQAMRNGILTS 300
KVCW R C DA++LAAFDDAIADGVDILS+SIGG VRPFF +GIAIGA+QAMRNGIL S
Sbjct: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
Query: 301 NSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGNDGPNWYTTGS+APWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP
Sbjct: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
Query: 361 LVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGT 420
LVYAGDVPNIGGGF+S+ISRFC VNSVDRNLVRGKIVLCDSS SFPSLNGAVGVVME T
Sbjct: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
Query: 421 TTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGP 480
+W LI TFPLPASHFNPPSANPIRLYITSNPAPTATIFRSV VN TNAPVAA FSSRGP
Sbjct: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
Query: 481 NAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVK 540
N ISPNILKPDLSAPGVNILAAWPQNVPIS G PLSSPYNFASGTSMACPH TAIAAYVK
Sbjct: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
Query: 541 TFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVE 600
TF+PWWSPAAIKSALMTTA PMD + ++ AEF+YGSGQINPLKAVNPGLVYNASESDYVE
Sbjct: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
Query: 601 YLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNV 660
YLCGIP IT AQL+ ITGEDIICTDSNTGRDLDLNYPSFAL MGRGQSLRVFFPRTLTNV
Sbjct: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
Query: 661 EATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLV 720
EATVSTYRATVYSPPGLN+QFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP TVSASLV
Sbjct: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
Query: 721 WSNGVREVRSPIVLF 735
WSNGVREVRSPIVLF
Sbjct: 721 WSNGVREVRSPIVLF 735
BLAST of CmaCh04G011570 vs. NCBI nr
Match:
XP_023547983.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 670/731 (91.66%), Postives = 688/731 (94.12%), Query Frame = 0
Query: 5 ALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGSAFSA 64
ALR+VFLNLL TL+I+ SASNGDGRKIYIVYMGSKPKDSSQ HLHHRAMLEEVVGSAFSA
Sbjct: 121 ALRVVFLNLLCTLIISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGSAFSA 180
Query: 65 ESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTRDVPR 124
ESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGF DVPR
Sbjct: 181 ESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFPPDVPR 240
Query: 125 IPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGARAYRA 184
+PQQES+IIVGV D+GIWPDSLSF DQGLGPPPPKWKGTCEASANFRCNRKIIGARAYRA
Sbjct: 241 VPQQESNIIVGVLDSGIWPDSLSFDDQGLGPPPPKWKGTCEASANFRCNRKIIGARAYRA 300
Query: 185 VGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVYKVCW 244
GPLPPTEAWGPIDT+GHGTHVASTVAGRLVDNA LYGFG+GLARGGVPSARIAVYKVCW
Sbjct: 301 EGPLPPTEAWGPIDTSGHGTHVASTVAGRLVDNAGLYGFGVGLARGGVPSARIAVYKVCW 360
Query: 245 -GRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDGIAIGAYQAMRNGILTSNSAG 304
C DA++LAA DDAIADGVDILSLSI LVRPFF D IAIGAYQAMRNGILTSNSAG
Sbjct: 361 ENGWCSDANILAAIDDAIADGVDILSLSITSLVRPFFEDAIAIGAYQAMRNGILTSNSAG 420
Query: 305 NDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVYA 364
N GP+W TTGS APWMLSVAANTIDRNF + VQLGNGNVYQGVAINLFDLGGKQYPLVYA
Sbjct: 421 NRGPDWSTTGSTAPWMLSVAANTIDRNFQSQVQLGNGNVYQGVAINLFDLGGKQYPLVYA 480
Query: 365 GDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGTTTWQ 424
GDVPNI GGF+S+ SRFCGVNSVDRNLVRGKIVLCDSS SFPSLNGAVGVVME TTTW
Sbjct: 481 GDVPNIAGGFSSSTSRFCGVNSVDRNLVRGKIVLCDSSAVSFPSLNGAVGVVMESTTTWH 540
Query: 425 LIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPNAIS 484
LI TFPLPASHFNPPSANPIRLYITSNPAPTATIFRSV VN TNAPVAADFSSRGPNAIS
Sbjct: 541 LIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAADFSSRGPNAIS 600
Query: 485 PNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKTFNP 544
PNILKPDLSAPGVNILAAWPQNVPIS G PLSSPYNFASGTSM+CPH TAIA YVKTF+P
Sbjct: 601 PNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMSCPHATAIAVYVKTFSP 660
Query: 545 WWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVEYLCG 604
WWSPAAIKSALMTTA PMD KVN QAEFS+GSGQINPLKAVNPGLVYNASESDYVEYLCG
Sbjct: 661 WWSPAAIKSALMTTASPMDAKVNTQAEFSHGSGQINPLKAVNPGLVYNASESDYVEYLCG 720
Query: 605 IPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVEATV 664
IPGIT AQLRLITGEDIICTD NTGRD+DLNYPSFALFMGRGQSLRVFFPRTLTNVEATV
Sbjct: 721 IPGITRAQLRLITGEDIICTDINTGRDVDLNYPSFALFMGRGQSLRVFFPRTLTNVEATV 780
Query: 665 STYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVWSNG 724
STYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP TVSASLVWSNG
Sbjct: 781 STYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVWSNG 840
Query: 725 VREVRSPIVLF 735
VREVRSPIVLF
Sbjct: 841 VREVRSPIVLF 851
BLAST of CmaCh04G011570 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 598.6 bits (1542), Expect = 6.8e-171
Identity = 325/705 (46.10%), Postives = 439/705 (62.27%), Query Frame = 0
Query: 36 MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG 95
MG+ P+ HH ++L+++VG+ ++ +V SYKRSFNGFA L++ E+Q++ + +
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 96 VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP 155
VVSVFPSK+++L TTRSWDF+GF R +ESD+IVGV D+GIWP+S SF D+G GP
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 156 PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV 215
PP KWKG+C+ F CN K+IGAR Y A D GHGTH AST AG V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKF-------ADSARDEEGHGTHTASTAAGNAV 180
Query: 216 DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGG- 275
AS YG G ARGGVPSARIA YKVC+ R C D D+LAAFDDAIADGVD++S+SI
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISAD 240
Query: 276 LVRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAH 335
V + +AIG++ AM GI+T+ SAGN+GP+ + +++PWM++VAA+ DR F
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300
Query: 336 VQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGK 395
V LGNG G+++N F+L G ++P+VY N+ + + +C VD LV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSELVKGK 360
Query: 396 IVLCDS-SGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAP 455
IVLCD G L GA+GV+++ T P PAS I+ YI S P
Sbjct: 361 IVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPP 420
Query: 456 TATIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAW-PQNVP---IS 515
A I R+ E+ AP FSSRGP+ + N+LKPD+SAPG+ ILAA+ P P ++
Sbjct: 421 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 480
Query: 516 PGDPLSSPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQA 575
P D S Y+ SGTSMACPHV +AAYVK+F+P WSP+AIKSA+MTTA PM++K N +
Sbjct: 481 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 540
Query: 576 EFSYGSGQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGR 635
EF+YGSGQINP KA +PGLVY DY++ LC G L +G+++ C++ +
Sbjct: 541 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCA-EGFDSTTLTTTSGQNVTCSERTEVK 600
Query: 636 DLDLNYPSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTF 695
DLNYP+ F+ V F RT+TNV STY+A+V P +Q ++P L F
Sbjct: 601 --DLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVV-PLQPELQISIEPEILRF 660
Query: 696 HGIGDTKSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 735
+ + KSF + + G D + VS+S+VWS+G VRSPIV +
Sbjct: 661 GFLEEKKSFVVTISG-KELKDGSFVSSSVVWSDGSHSVRSPIVAY 689
BLAST of CmaCh04G011570 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 584.7 bits (1506), Expect = 1.0e-166
Identity = 332/739 (44.93%), Postives = 454/739 (61.43%), Query Frame = 0
Query: 9 VFLNLLFTLLITTSASNGD--GRKIYIVYMGSKPKDSSQAHL-HHRAMLEEVVGSAFSAE 68
+F +LL L + SA D +++YIVY+GS P + H ++L+E+ G +
Sbjct: 10 LFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIEN 69
Query: 69 SIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTRDV--P 128
+V SYK+SFNGFA +LTE E +R+A E VVSVFPS+ KLQTT SW+F+G +
Sbjct: 70 RLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTK 129
Query: 129 RIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGARAYR 188
R ESD I+GV D+GI+P+S SF DQG GPPP KWKGTC NF CN K+IGAR Y
Sbjct: 130 RTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYT 189
Query: 189 AVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVYKVC 248
A T D +GHGTH AS AG V N++ YG G G ARGGVP+ARIAVYKVC
Sbjct: 190 AKSKANQTAR----DYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC 249
Query: 249 WGRTCEDADMLAAFDDAIADGVDILSLSIG-GLVRPFFHDGIAIGAYQAMRNGILTSNSA 308
C+ M++AFDDAIADGVD++S+SI + PF D IAIGA+ AM G+LT N+A
Sbjct: 250 DNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAA 309
Query: 309 GNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVY 368
GN+GP T S APW+ SVAA+ +R F A V LG+G + G ++N +D+ G YPLVY
Sbjct: 310 GNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVY 369
Query: 369 AGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSS-GASFPSLNGAVG-VVMEGTT 428
+ + +R C +D LV+GKIVLCDS+ G GAVG +V
Sbjct: 370 GKSAAL--STCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEP 429
Query: 429 TWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPN 488
IR+FP+ S + + Y+ S P AT+ +S E+++ AP+ A FSSRGP+
Sbjct: 430 DRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPS 489
Query: 489 AISPNILKPDLSAPGVNILAAW-PQNVPI-SPGDPLSSPYNFASGTSMACPHVTAIAAYV 548
+I +ILKPD++APGV ILAA+ P + P S D Y+ SGTSMACPHV +AAYV
Sbjct: 490 SIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYV 549
Query: 549 KTFNPWWSPAAIKSALMTTAFPMDVKVNR--QAEFSYGSGQINPLKAVNPGLVYNASESD 608
KTF+P WSP+ I+SA+MTTA+PM+ + EF+YGSG ++P+ A+NPGLVY +++D
Sbjct: 550 KTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKAD 609
Query: 609 YVEYLCGIPGITPAQLRLITGEDIICT-DSNTGRDLDLNYPSFALFMGRGQSLRVFFPRT 668
++ +LCG+ T LR+I+G++ CT + + +LNYP+ + + + + F RT
Sbjct: 610 HINFLCGL-NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRT 669
Query: 669 LTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVS 728
+TNV STY A V PG + KV P L+ + + +SF + V T VS
Sbjct: 670 VTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT-KQPVS 729
Query: 729 ASLVWSNGVREVRSPIVLF 735
A+L+WS+G VRSPI+++
Sbjct: 730 ANLIWSDGTHNVRSPIIVY 738
BLAST of CmaCh04G011570 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 582.8 bits (1501), Expect = 3.9e-166
Identity = 322/742 (43.40%), Postives = 447/742 (60.24%), Query Frame = 0
Query: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHL-HHRAMLEEVVG 60
M +LA L+ L L +++ ++ D +++YIVYMGS + H +L+EV G
Sbjct: 1 MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60
Query: 61 SAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFT 120
+ +V SYKRSFNGFA +LTE E +R+A GVVSVFP+K +LQTT SWDF+G
Sbjct: 61 ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120
Query: 121 RDV--PRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKII 180
+ R P ESD I+GV D+GI P+S SF D+G GPPP KWKG C NF CN K+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180
Query: 181 GARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARI 240
GAR Y + G D +GHGTH AST AG V +AS +G G G RGGVP++R+
Sbjct: 181 GARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 240
Query: 241 AVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGGLVRPFF-HDGIAIGAYQAMRNGI 300
A YKVC C +L+AFDDAIADGVD++++SIG F +D IAIGA+ AM G+
Sbjct: 241 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 300
Query: 301 LTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGK 360
LT NSAGN GP + +APW+L+VAA+T +R F V LGNG G ++N +++ GK
Sbjct: 301 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 360
Query: 361 QYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDS-SGASFPSLNGAVGVV 420
YPLVY + ++ + C ++ VD++ V+GKI++C G GAVG++
Sbjct: 361 DYPLVYGKSAAS--SACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLI 420
Query: 421 MEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFS 480
T + PLPA+ + Y+ S +P A + ++ + + +PV A FS
Sbjct: 421 YR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 480
Query: 481 SRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIA 540
SRGPN I+ +ILKPD++APGV ILAA+ S D Y+ SGTSM+CPHV +A
Sbjct: 481 SRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVA 540
Query: 541 AYVKTFNPWWSPAAIKSALMTTAFPMDVKVN--RQAEFSYGSGQINPLKAVNPGLVYNAS 600
AYVKTFNP WSP+ I+SA+MTTA+P++ EF+YGSG ++P+ A NPGLVY
Sbjct: 541 AYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELD 600
Query: 601 ESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSF-ALFMGRGQSLRVFF 660
+SD++ +LCG+ T L++I+GE + C+++ +LNYPS A G G + V F
Sbjct: 601 KSDHIAFLCGM-NYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 660
Query: 661 PRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPT 720
RTLTNV STY + V + G + K+ P L+F + + +SFT+ V G +
Sbjct: 661 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTG-SNLDSEV 720
Query: 721 TVSASLVWSNGVREVRSPIVLF 735
SA+L+WS+G VRSPIV++
Sbjct: 721 PSSANLIWSDGTHNVRSPIVVY 728
BLAST of CmaCh04G011570 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 574.3 bits (1479), Expect = 1.4e-163
Identity = 328/746 (43.97%), Postives = 446/746 (59.79%), Query Frame = 0
Query: 5 ALRLVFLNLLFTLLITTSASNG---DGRKIYIVYMGSKPKDSSQAHL-HHRAMLEEVVGS 64
A+ L+ +F LL+ + AS G +++YIVYMG+ P + HH ++L++V G
Sbjct: 4 AVSYCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGE 63
Query: 65 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFT- 124
+ + +V +YKRSFNGFA +LTE E + +A + VVSVFPSKN LQTT SW+F+G
Sbjct: 64 SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 123
Query: 125 -RDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIG 184
+ R P ESD I+GV D+GI+P+S SF +G GPPP KWKG C+ NF CN K+IG
Sbjct: 124 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 183
Query: 185 ARAYRAVGPLPPTEAW--GPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSAR 244
AR Y P E + D GHG+H AS AG V + S YG G G RGGVP+AR
Sbjct: 184 ARYY-----TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAAR 243
Query: 245 IAVYKVCWGRT--CEDADMLAAFDDAIADGVDILSLSIGG-LVRPFFHDGIAIGAYQAMR 304
IAVYKVC C +LAAFDDAIAD VDI+++S+G V F D +AIGA+ AM
Sbjct: 244 IAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMA 303
Query: 305 NGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDL 364
GILT N AGN+GP T S+APW+ +VAA+ ++R F V LGNG G ++N FDL
Sbjct: 304 KGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDL 363
Query: 365 GGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSS-GASFPSLNGAV 424
GK+YPLVY + +++ + FC +D V+GKIVLCD+ GAV
Sbjct: 364 NGKKYPLVYG---KSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAV 423
Query: 425 GVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAA 484
++ F P S + N + Y+ S P A + +S + + APV A
Sbjct: 424 ASIVRNPYE-DAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 483
Query: 485 DFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVT 544
+SSRGPN + +ILKPD++APG ILAA+ VP S D Y SGTSM+CPHV
Sbjct: 484 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSESDTRHVKYTVISGTSMSCPHVA 543
Query: 545 AIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKV---NRQAEFSYGSGQINPLKAVNPGLV 604
+AAY+KTF+P WSP+ I+SA+MTTA+PM+ N AEF+YG+G ++P+ A++PGLV
Sbjct: 544 GVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLV 603
Query: 605 YNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNT-GRDLDLNYPSFALFMGRGQSL 664
Y A++SD++ +LCG T +LRLI+G+ CT T +LNYPS + + +
Sbjct: 604 YEANKSDHITFLCGF-NYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPF 663
Query: 665 RVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSA 724
+V F RT+TNV +TY+A V G ++ KV P L+ + + KSFT+ V G
Sbjct: 664 KVTFRRTVTNVGRPNATYKAKVV---GSKLKVKVVPAVLSLKSLYEKKSFTVTVSG-AGP 723
Query: 725 TDPTTVSASLVWSNGVREVRSPIVLF 735
VSA L+WS+GV VRSPIV++
Sbjct: 724 KAENLVSAQLIWSDGVHFVRSPIVVY 735
BLAST of CmaCh04G011570 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 572.8 bits (1475), Expect = 4.0e-163
Identity = 322/733 (43.93%), Postives = 440/733 (60.03%), Query Frame = 0
Query: 13 LLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHL----HHRAMLEEVVGSAFSAESIV 72
LL LL + SA + ++YIVYMGS SS+A H ++L++V G + +V
Sbjct: 14 LLVLLLSSVSAIIDEDTQVYIVYMGSL---SSRADYIPTSDHMSILQQVTGESSIEGRLV 73
Query: 73 YSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFT--RDVPRIP 132
SYKRSFNGFA +LTE E +A+ EGVVSVFP+K +L TT SWDF+G ++ R
Sbjct: 74 RSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNL 133
Query: 133 QQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGARAYRAVG 192
ESD I+GV DTGIWP+S SF D+G GPPP KWKG C NF CN K+IGAR Y + G
Sbjct: 134 AIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG 193
Query: 193 PLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVYKVCWGR 252
DT+GHGTH AST AG V + S +G G G RGGVP++RIA YKVC
Sbjct: 194 TR---------DTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDS 253
Query: 253 TCEDADMLAAFDDAIADGVDILSLSIGGLVRPFFHDG-IAIGAYQAMRNGILTSNSAGND 312
C +L++FDDAIADGVD++++SIG F D IAIGA+ AM GILT +SAGN
Sbjct: 254 GCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNS 313
Query: 313 GPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVYAGD 372
GP T +APW+ +VAA+T +R F V LGNG G ++N FD+ GK+YPLVY
Sbjct: 314 GPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKS 373
Query: 373 VPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDS-SGASFPSLNGAVGVVMEGTTTWQL 432
+ ++ + C ++++ V+GKI++C SG GA+ ++ + + +
Sbjct: 374 AAS--SACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAII-DKSPRPDV 433
Query: 433 IRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGPNAISP 492
T LPAS + YI S +P A + ++ + + +PV A FSSRGPN I+
Sbjct: 434 AFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAV 493
Query: 493 NILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVKTFNPW 552
+ILKPD++APGV ILAA+ N S D Y+ SGTSMACPHV +AAYVKTF P
Sbjct: 494 DILKPDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPR 553
Query: 553 WSPAAIKSALMTTAFPMDVKVN--RQAEFSYGSGQINPLKAVNPGLVYNASESDYVEYLC 612
WSP+ I+SA+MTTA+P+ K EF+YG+G ++P+ A+NPGLVY ++D++ +LC
Sbjct: 554 WSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLC 613
Query: 613 GIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSF-ALFMGRGQSLRVFFPRTLTNVEA 672
G+ T L++I+G+ + C+ N +LNYPS A G + V F RTLTNV
Sbjct: 614 GM-NYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGT 673
Query: 673 TVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVWS 732
STY++ V + G + KV P L F + + +SF++ V G SA+L+WS
Sbjct: 674 PNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG-SDVDSEVPSSANLIWS 729
Query: 733 NGVREVRSPIVLF 735
+G VRSPIV++
Sbjct: 734 DGTHNVRSPIVVY 729
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 3.5e-236 | 57.74 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 9.3e-173 | 45.49 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FGU3 | 1.4e-165 | 44.93 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
| Q9FIG2 | 5.5e-165 | 43.40 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q9STF7 | 1.9e-162 | 43.97 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1IMT3 | 0.0e+00 | 100.00 | cucumisin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1 | [more] |
| A0A6J1IP33 | 0.0e+00 | 100.00 | cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1 | [more] |
| A0A6J1GX71 | 0.0e+00 | 91.16 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458261 PE=3 SV=1 | [more] |
| A0A6J1CBL9 | 5.0e-254 | 60.70 | LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC1110101... | [more] |
| K7NBW1 | 1.2e-252 | 61.52 | Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1 | [more] |
