CmaCh04G010850 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G010850
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	alpha-soluble NSF attachment protein 2-like
Location	Cma_Chr04: 5590125 .. 5593726 (+)
RNA-Seq Expression	CmaCh04G010850
Synteny	CmaCh04G010850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTTTTTTTTTTTTTTTTTTTTTTCCCGTTTCTCGATTCACATTCGTCCATGGCGGATCATTTGGCCAGAGCAGAGGATTTCGATTCCAAAGCAGAAAAGAAGCTCAGTGGTTGGGGATTGTTCGGCTCCAAGTACGATGATGCTGCAGATCTCTTCGATAAAGCCGCCAATTCTTACAAGCTTTCGAAATCATGTATTGCTAATCGCTTTTCACCTTATGATTCTTCTTTTTATTTCCCTCTGTTCGATCATTACTTTCCCTGTTTTGTTAATTTCATTATCCCTAAACGCTGCTGCATATGAGGACGGTCAGGATTTTTGTTGTTTCATTGTTTTTATCATTTTGATGCGTAATTTAATTGTGGCGGGCTACTTGTGTTCTGTTTCTTTTTGCTTCTTTTATGATATCGTTTTTCCCGTTTTCTGGCGGTTTTTTTGGGTTATGCGAGAGTTTTTGCTTAGAAATCAAGAGCACCATGATTTCAACTTTTGATTGATCGGCATTGTTGCATGCTTGGTAGGGGCGGAGCCAAAAATTTTCATGATGAGGAGAAAAGTTTTACATTCTTATATATAATCTTTGGAGTTTAAAGTTAGGAGGTAAAATAAAACTTGTGAGATCACACGTTGGTTGGAGAGGGAAGAAATATTTCTTATAAGGGTGTAGAAACCTCTCCCTAACATACACGTTTTAAAACCGTGAGACTGATAGCGATACGTAACGAGCCAAAGCGGACAATATTTGTTAGTAGTGGGCTTGAGCGGTTACAAATAATATCAGAGCCAAACACCAAGAGGTGTGCCAATGGGGATGCTGGTCCCCCAAGGAGGTGGATTGTGAGATCCCACATCGGTTGGAGAAGGAAACAAAGCATTTCTTATTGTCTATTTCAGTTTCCTGCCATCCATTGCCTTCTAATCTCTGTGGATGTGGTTTTCCAGGGGATAAAGCAGGATCAACGTATATAAAGTTAGCAAATTGTCATCTGAAGGTTGGTGCTTCGTGTTTGTGAGATCAATGGCTTCGTCTTCCTTTGTTGTACACGGTTTCTTTTACTGAACAATGCATTGTTTTTCTTATAAAGATGGAAAGCAAACATGAAGCAGCTCAAGCTTATATTGATGCGGGCAATTGCTATAAGAAAACATCTGTTAGAGGTAATACTGGTTCATAATTGATCATTCTATACTTCAACGCGAACTGTTCGAGTGAAACAATGCTAGTTCAATTATCGACGTTATTGTTGGTTAAATTGCTTATTTCCAATTGTTATCTTGATGCAGAGGCCATATCTTGCATAGAGCAGGCAGTGAATTTGTTTTGTGACATTGGAAGACTCAATATGGCTGCAAGATATTACAAGGTTCATTGTTATTCTGCTACGTGAAATGCTTATTATTTGATTTCTTTGATCATCTAAAATGTTCGGTTACTTCACGAGGTTATGCTGTGAATTGTTTTTTTCTTTTCTTTTCTTTTGGAACCTATTCTTGTGGCTGCTAATTCTTGATTAGACACTATGAAGTATCATACATTATGTAACATCCCAAGCTCACCGCTAGCAGATATTGTCCTCGTTGGACTTTCCCTTTCGAGCTTCTCCGGAAGGCTTTTAAAATGCATCTGCTAGGGATGGGCTTGGAATGTTACAAATGGTATCAAAGCCAGACATTGGGCGGTTTACCAGCATGAATGCTGAGCCCCGAAGGAGGGTGGATTGTGAGATCCCCCATCAATTGGGGAGGAGAACAAAACATTATTCATAAGGATATGGAAACCTCTCCTTAGTAAACACGCTTTAAAACTGTGATGTTGACAGCGATAGTAACCTGCCAAAGAAGACAATATCCGCTAGCAGTGGGCTTGAACTGTTACACATGGCAATTTCTTATTGCAGAATTCTTTCATCATTTGATTGACAGAGAATTAAACAAATGCCTCATATGCAATCCATGACTTGGATTTACCTTCTCGTGTGTGCAGGAAATTGCTGAATTGTTCGAGTCCGAACAGGATATCGAGCAAGCAATCGATTACTTTGAAAAAGCTGCTGACCTTTTCCAAAATGAAGAAGTGACAACTTCAGCAAATCAATGCAAGCAGAAAGTTGCACAATTTGCTGCACAGTTGGAACAGTAAGATAGTGTAGTATTGATAAATTGTGTTGCATCCTTATGATTGACACATTTAAAGCATTACCAAGTTTTCTTCTACTTTGATGGTTTCTTCTGGTTTCATATTATCCATTTATTTTGACATTGTGCAAACATTTATCTTATGAAAACATTTTTGAATGGTAACATAATTTGTAGGTATCAGAAAGCAATCGATATCTACGAAGAGATAGCGAAGTATTCTCTGAAGAGTAACTTGCTCAAGTATGGAGTGAAAGGGCATCTTCTTAATGCTGGAATTTGCCAGCTTTGCAAAGGCGATATCGTTGCTATTACCAGAGCATTGGATAACTATCAGGTAGTTATCGGACATGACTGTCTCATCTCGTCCTCGTGATTTCTGTCCTTACCATATTACATCCCTTCTGCAGGACCTAGATCCTACATTTTCAGGGTCACGTGAATATAAGCTTTTAGCGGTGCGTTACGTTCACCCCTGAATTCTGATTTATTATTCTTTCTTTTGCATATTTAATGTTTTTTCATATATGTTGAACAAGAGGTTTGATGGTTCTACTCATTTCTTGTCCGGCTTGCTCATTGATTGAAGGCATTGTCATATATTGCATGAAATTGTAGTGCTTCTTGACTAGAAAACCTCTTATATTTGTTCGTTTATCCGTCTGATAATTTTACCTCAGGACATTGCATCTGCCATTGATGAGGAAGATGTAGCAAAATTTACTGATGTTGTCAAGGAATTTGATAGCATGACCACACTGGTAAGATCATACTTCATGAGTCGACTTGAATGCTATGTTATTGATATGTAATCTGCGAAGTTACGTCCTGAAAATCTTACGTTCGATCTAGAGAATGTTCGGTATCCAACTCGGAATCACCTGAAAATAACACGAAAACACACACAAACCAGAGGGAAACAAATACCAAATTGTGTTATATTGATAGTTCTTCAATATACAGCACAATCACACCCCATGCTGAAATCCCAACCCCCCACACACACGCATGTTCCTCCTGCCCCCGTAACTTACTACCTACTGTGAAATAACATTCTTACCGTTCGATTGGAGTCTTCACACAAGTGCAGTTTGATCGTTTGAAATAACTTTGTTGTTTTCATTCGTAATGGTAAAGATTAATGTGCACAAACTATTGACAGGATCCATTGAAGACGACCCTTCTTTTAAGGGTGAAGGACAAGCTGAAAGCCAAAGAGCTGGAAGAGGACGATCTTACCTAAAGAGGATGATTTGTTTTTTAGATTCTTTCTATGGCTGTGTGTGTGTGTGTGTGTGTGTGTGTTCTATGTCCAGATGTTTTGGCAATCGACTGTCTATTCATTATTGAATGACTTGGTGTTTGGGTTTGGGTTTGGATTTTGATGAATAAAGTCTCATCCTCTAAGCTGTGAGCTTCTGAGTGTTATTCTCATTTTGGAAACCAAATCTCTACAAAAAAAAAA

mRNA sequence

TGTTTTTTTTTTTTTTTTTTTTTTCCCGTTTCTCGATTCACATTCGTCCATGGCGGATCATTTGGCCAGAGCAGAGGATTTCGATTCCAAAGCAGAAAAGAAGCTCAGTGGTTGGGGATTGTTCGGCTCCAAGTACGATGATGCTGCAGATCTCTTCGATAAAGCCGCCAATTCTTACAAGCTTTCGAAATCATGGGATAAAGCAGGATCAACGTATATAAAGTTAGCAAATTGTCATCTGAAGATGGAAAGCAAACATGAAGCAGCTCAAGCTTATATTGATGCGGGCAATTGCTATAAGAAAACATCTGTTAGAGAGGCCATATCTTGCATAGAGCAGGCAGTGAATTTGTTTTGTGACATTGGAAGACTCAATATGGCTGCAAGATATTACAAGGAAATTGCTGAATTGTTCGAGTCCGAACAGGATATCGAGCAAGCAATCGATTACTTTGAAAAAGCTGCTGACCTTTTCCAAAATGAAGAAGTGACAACTTCAGCAAATCAATGCAAGCAGAAAGTTGCACAATTTGCTGCACAGTTGGAACAGTATCAGAAAGCAATCGATATCTACGAAGAGATAGCGAAGTATTCTCTGAAGAGTAACTTGCTCAAGTATGGAGTGAAAGGGCATCTTCTTAATGCTGGAATTTGCCAGCTTTGCAAAGGCGATATCGTTGCTATTACCAGAGCATTGGATAACTATCAGGACCTAGATCCTACATTTTCAGGGTCACGTGAATATAAGCTTTTAGCGGACATTGCATCTGCCATTGATGAGGAAGATGTAGCAAAATTTACTGATGTTGTCAAGGAATTTGATAGCATGACCACACTGGATCCATTGAAGACGACCCTTCTTTTAAGGGTGAAGGACAAGCTGAAAGCCAAAGAGCTGGAAGAGGACGATCTTACCTAAAGAGGATGATTTGTTTTTTAGATTCTTTCTATGGCTGTGTGTGTGTGTGTGTGTGTGTGTGTTCTATGTCCAGATGTTTTGGCAATCGACTGTCTATTCATTATTGAATGACTTGGTGTTTGGGTTTGGGTTTGGATTTTGATGAATAAAGTCTCATCCTCTAAGCTGTGAGCTTCTGAGTGTTATTCTCATTTTGGAAACCAAATCTCTACAAAAAAAAAA

Coding sequence (CDS)

ATGGCGGATCATTTGGCCAGAGCAGAGGATTTCGATTCCAAAGCAGAAAAGAAGCTCAGTGGTTGGGGATTGTTCGGCTCCAAGTACGATGATGCTGCAGATCTCTTCGATAAAGCCGCCAATTCTTACAAGCTTTCGAAATCATGGGATAAAGCAGGATCAACGTATATAAAGTTAGCAAATTGTCATCTGAAGATGGAAAGCAAACATGAAGCAGCTCAAGCTTATATTGATGCGGGCAATTGCTATAAGAAAACATCTGTTAGAGAGGCCATATCTTGCATAGAGCAGGCAGTGAATTTGTTTTGTGACATTGGAAGACTCAATATGGCTGCAAGATATTACAAGGAAATTGCTGAATTGTTCGAGTCCGAACAGGATATCGAGCAAGCAATCGATTACTTTGAAAAAGCTGCTGACCTTTTCCAAAATGAAGAAGTGACAACTTCAGCAAATCAATGCAAGCAGAAAGTTGCACAATTTGCTGCACAGTTGGAACAGTATCAGAAAGCAATCGATATCTACGAAGAGATAGCGAAGTATTCTCTGAAGAGTAACTTGCTCAAGTATGGAGTGAAAGGGCATCTTCTTAATGCTGGAATTTGCCAGCTTTGCAAAGGCGATATCGTTGCTATTACCAGAGCATTGGATAACTATCAGGACCTAGATCCTACATTTTCAGGGTCACGTGAATATAAGCTTTTAGCGGACATTGCATCTGCCATTGATGAGGAAGATGTAGCAAAATTTACTGATGTTGTCAAGGAATTTGATAGCATGACCACACTGGATCCATTGAAGACGACCCTTCTTTTAAGGGTGAAGGACAAGCTGAAAGCCAAAGAGCTGGAAGAGGACGATCTTACCTAA

Protein sequence

MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLANCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAELFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAKYSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT

Homology

[Close]

BLAST of CmaCh04G010850 vs. ExPASy Swiss-Prot
Match: Q9SPE6 (Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2 PE=1 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.1e-124
Identity = 218/289 (75.43%), Postives = 254/289 (87.89%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M DHL RAE+F+ KAEKKL+GWG+FGSKY+DAADL +KAANSYKL+KSWD+AG  Y+KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           +CHLK +SKH+AA AY +A  CYKK    EA SC+E+AVN+FC+IGRLNMAARYYKEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
            +ES+Q  EQAI YFEKAA+ FQNEEVTTSANQC  KVAQ+AAQLEQY+KAI IYE+IA+
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           +SL +NLLKYGVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTF+G+RE K LAD+AS
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLADLAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEED+AKFTDVVKEFDSMT LD  KTT+LLRVK+KLKAKELEEDDLT
Sbjct: 241 AIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. ExPASy Swiss-Prot
Match: P93798 (Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 1.9e-121
Identity = 216/289 (74.74%), Postives = 251/289 (86.85%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MAD++ R E+F+ KAEKK+SGWGLFGSKY+DAAD +DKAAN +KL+KSWD+AGSTY+KL+
Sbjct: 1   MADNIQRGEEFEKKAEKKISGWGLFGSKYEDAADFYDKAANCFKLAKSWDRAGSTYVKLS 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
                 +SKHEAAQAY DAG+CYKKTS +EAISC+EQA  LF D GR NMA +YYKEIAE
Sbjct: 61  TVIQSSDSKHEAAQAYADAGHCYKKTSAKEAISCLEQAAYLFLDNGRFNMAGKYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E EQ+ EQAI YFEKAAD++Q+EE TT+ANQC  KVAQFAAQLEQYQKAI IYE+I +
Sbjct: 121 LYELEQNFEQAIIYFEKAADIYQSEEATTAANQCNAKVAQFAAQLEQYQKAIQIYEDIGR 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
            SL +NLLKYGVKGHLLNAGICQLCKGD+VAIT ALD YQ++DPTFSG+REYKLL D+A+
Sbjct: 181 PSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALDRYQEMDPTFSGTREYKLLVDLAA 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           A+DEEDV KFTD VKEFDSMT LD  KTTLLLRVK+ +KAKELEEDDLT
Sbjct: 241 AVDEEDVVKFTDAVKEFDSMTQLDAWKTTLLLRVKEAIKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. ExPASy Swiss-Prot
Match: Q9M5P8 (Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.5e-113
Identity = 202/289 (69.90%), Postives = 248/289 (85.81%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M DH+AR EDF++KAEKKL GW LFGSKY+DAADLFDKA N +KL+KSWDKAG+ Y+KLA
Sbjct: 1   MGDHIARGEDFENKAEKKLGGWALFGSKYEDAADLFDKAGNCFKLAKSWDKAGAVYVKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLK++SKHEAA AY DA +CYKK++++EA+SC+EQAVN F DIGRLNM+ARYYKEIAE
Sbjct: 61  NCHLKLDSKHEAANAYADAAHCYKKSNIKEAVSCLEQAVNFFLDIGRLNMSARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E EQ+++QAI Y     +   +E+V+   NQC +K+AQF+AQ E+Y KAI+I+EEIA+
Sbjct: 121 LYEQEQNLDQAIRYAVYEINP-GSEDVSAPPNQCLKKIAQFSAQNEKYPKAIEIFEEIAR 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           +S+ +NLLKYGV+ HLLNAGICQLCKGD+V I  AL+ YQ+LDPTFSG+RE KLL D+A+
Sbjct: 181 HSVNNNLLKYGVREHLLNAGICQLCKGDVVPINNALERYQELDPTFSGTRECKLLVDLAA 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEEDVAKFTD VKE+DSMT LD  +TTLLLRVK+ LKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDAVKEYDSMTQLDAWRTTLLLRVKETLKAKELEEDDLT 288

[Close]

BLAST of CmaCh04G010850 vs. ExPASy Swiss-Prot
Match: Q9LXZ5 (Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.7e-61
Identity = 135/253 (53.36%), Postives = 166/253 (65.61%), Query Frame = 0


Query: 11  FDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLANCHLKMESKH 70
           F  KAEKKL+    F +KY+DAADL +KA +SYKL+KSWD+AG  Y+KLA+CHLK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAELFESEQDIEQ 130
             A  Y+    C                                  EIAE +ES++  EQ
Sbjct: 159 -LANTYMIMDECMD-------------------------------HEIAEYYESDEMFEQ 218

Query: 131 AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAKYSLKSNLLKY 190
           AI Y+E AA+ FQ EEVTTSANQC  KVAQ+A+QLEQ  +     +  A++SL + LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSR---FMKTQARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIASAIDEEDVAKF 250
           GVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTFSG+RE K LAD+ASAIDEED+AKF
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLADLASAIDEEDIAKF 314

Query: 251 TDVVKEFDSMTTL 264
           TDV KE DS++ L
Sbjct: 339 TDVSKEIDSVSPL 314

[Close]

BLAST of CmaCh04G010850 vs. ExPASy Swiss-Prot
Match: Q54NP6 (Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snpA PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.4e-60
Identity = 123/279 (44.09%), Postives = 187/279 (67.03%), Query Frame = 0


Query: 6   ARAEDFDSKAEKKLSGWGLF------GSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKL 65
           A+A++F   A+K+L G   F       S+YDDAA  + KAAN +K+SK WD+AG+ + K 
Sbjct: 5   AKAKEFLDAADKRLRGGNFFKMFGGGSSRYDDAASDYTKAANLFKMSKKWDQAGAAFQKA 64

Query: 66  ANCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIA 125
           A C LK  SKH+AA +Y+ A  CYKK +V +AI+C++ A+  + D GR  ++A++ KEIA
Sbjct: 65  AECFLKGSSKHDAASSYVLAAGCYKKGNVIDAITCLKAAIEYYTDEGRFAISAKHQKEIA 124

Query: 126 ELFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIA 185
           EL+E+E D +QAI  ++ A+D F  E  T S++QC  K+A F+AQLE+Y+K+I+IYE++A
Sbjct: 125 ELYEAEGDFDQAIASYQIASDYFDGENSTVSSHQCLLKIALFSAQLERYEKSIEIYEQVA 184

Query: 186 KYSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIA 245
             SL +NL ++G K + L A +C L   D+V   RAL  Y+D+  +F+ +RE +LL  I 
Sbjct: 185 AASLDNNLTQWGCKEYFLRACLCYLAADDVVGAERALQRYKDMQASFNSTRECRLLDGII 244

Query: 246 SAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKL 279
            A    +V  FT+ V EF+S++ LD  KT++LLR+K+ +
Sbjct: 245 QACRNNNVEDFTNEVAEFNSISPLDSWKTSILLRIKNTI 283

[Close]

BLAST of CmaCh04G010850 vs. ExPASy TrEMBL
Match: A0A6J1JUA6 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488422 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 6.5e-157
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. ExPASy TrEMBL
Match: A0A6J1GYT0 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458118 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.4e-156
Identity = 288/289 (99.65%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAIT+ALDNYQDLDPTFSGSREYKLLADIAS
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. ExPASy TrEMBL
Match: A0A6J1CZQ2 (alpha-soluble NSF attachment protein 2-like OS=Momordica charantia OX=3673 GN=LOC111015967 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 3.7e-152
Identity = 275/289 (95.16%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARA+DF++KAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARADDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAY+DAGNCYKKTSV+EAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVKEAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E+EQD+E+AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LYENEQDVERAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           YSLK+NLLKYG KGHLLNAGICQLCKGDIVAIT+ALDNYQDLDPTFSGSREYKLLADIAS
Sbjct: 181 YSLKNNLLKYGAKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           A+DEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 ALDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. ExPASy TrEMBL
Match: A0A6J1HIF0 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464692 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.1e-135
Identity = 248/289 (85.81%), Postives = 271/289 (93.77%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MAD LA+ E+F+ KA KKL+GWGLFGSKY+DAADLFDKAANSYKL+KSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYTKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
            CHLK+ESKHEAAQAY+DA N YKKTS++EAISC+EQAVNLFCDIGRL+MAARY KEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ES+Q+IE AI YFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQY+KAI IYEEIA+
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           +SLKSNLLKYGVKGHLLNAG+CQLCKGDIVAIT+ALD YQDLDPTFSG+RE +LLADIA 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADIAG 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEEDV KFTDVVKEFDSMT LDP KTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPCKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. ExPASy TrEMBL
Match: A0A7N2R9Y3 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.1e-135
Identity = 244/289 (84.43%), Postives = 273/289 (94.46%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M D +ARAEDF+ KAEKKLSGWGLFGSKY+DAADLFDKAANS+KL KSWDKAG+TY+KLA
Sbjct: 1   MGDQIARAEDFEKKAEKKLSGWGLFGSKYEDAADLFDKAANSFKLGKSWDKAGATYVKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLK+ESKHEAAQAY+DA +CYKK+++ EAISC+EQAVN+FC+IGRL+MAARY+KEIAE
Sbjct: 61  NCHLKLESKHEAAQAYVDAAHCYKKSNINEAISCLEQAVNMFCEIGRLSMAARYFKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ESEQ+IEQAI YFEK+AD FQNEEVTTSANQCKQKVAQFAAQLEQYQK+I IYEEIA+
Sbjct: 121 LYESEQNIEQAIVYFEKSADFFQNEEVTTSANQCKQKVAQFAAQLEQYQKSIVIYEEIAR 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
            SL +NLLKYGVKGHLLNAGICQLCKGD+VAIT AL+ YQ+LDPTFSG+REYKLLADIAS
Sbjct: 181 QSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALERYQELDPTFSGTREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           A+DEEDVAKFTDVVKEFDSMT LD  KTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 ALDEEDVAKFTDVVKEFDSMTPLDSWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. NCBI nr
Match: XP_022991930.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita maxima])

HSP 1 Score: 563.1 bits (1450), Expect = 1.3e-156
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. NCBI nr
Match: XP_022956354.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023536437.1 alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo] >KAG6600896.1 hypothetical protein SDJN03_06129, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031530.1 hypothetical protein SDJN02_05570 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 562.0 bits (1447), Expect = 3.0e-156
Identity = 288/289 (99.65%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAIT+ALDNYQDLDPTFSGSREYKLLADIAS
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. NCBI nr
Match: XP_038893428.1 (alpha-soluble NSF attachment protein 2-like [Benincasa hispida])

HSP 1 Score: 555.4 bits (1430), Expect = 2.8e-154
Identity = 282/289 (97.58%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDF++KAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAY+DAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LYESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           YSLK+NLLKYGVKGHLLNAGICQLCKGDIVAIT+ALDNYQDLDPTFSG+REYKLLADIAS
Sbjct: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. NCBI nr
Match: XP_022146873.1 (alpha-soluble NSF attachment protein 2-like [Momordica charantia])

HSP 1 Score: 547.4 bits (1409), Expect = 7.6e-152
Identity = 275/289 (95.16%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARA+DF++KAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARADDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAY+DAGNCYKKTSV+EAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVKEAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E+EQD+E+AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LYENEQDVERAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           YSLK+NLLKYG KGHLLNAGICQLCKGDIVAIT+ALDNYQDLDPTFSGSREYKLLADIAS
Sbjct: 181 YSLKNNLLKYGAKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           A+DEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 ALDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. NCBI nr
Match: KAF3964963.1 (hypothetical protein CMV_010801 [Castanea mollissima])

HSP 1 Score: 493.4 bits (1269), Expect = 1.3e-135
Identity = 245/289 (84.78%), Postives = 273/289 (94.46%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M D +ARAEDF+ KAEKKLSGWGLFGSKY+DAADLFDKAANS+KL KSWDKAGSTY+KLA
Sbjct: 1   MGDQIARAEDFEKKAEKKLSGWGLFGSKYEDAADLFDKAANSFKLGKSWDKAGSTYVKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLK+ESKHEAAQAY+DA +CYKK+S+ EAISC+EQAVN+FC+IGRL+MAARY+KEIAE
Sbjct: 61  NCHLKLESKHEAAQAYVDAAHCYKKSSINEAISCLEQAVNMFCEIGRLSMAARYFKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ESE +IEQAI YFEK+AD FQNEEVTTSANQCKQKVAQFAAQLEQYQK+I+IYEEIA+
Sbjct: 121 LYESEHNIEQAIVYFEKSADFFQNEEVTTSANQCKQKVAQFAAQLEQYQKSIEIYEEIAR 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
            SL +NLLKYGVKGHLLNAGICQLCKGD+VAIT AL+ YQ+LDPTFSG+REYKLLADIAS
Sbjct: 181 QSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALERYQELDPTFSGTREYKLLADIAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           A+DEEDVAKFTDVVKEFDSMT LD  KTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 ALDEEDVAKFTDVVKEFDSMTPLDSWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. TAIR 10
Match: AT3G56190.1 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 446.0 bits (1146), Expect = 2.2e-125
Identity = 218/289 (75.43%), Postives = 254/289 (87.89%), Query Frame = 0


Query: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M DHL RAE+F+ KAEKKL+GWG+FGSKY+DAADL +KAANSYKL+KSWD+AG  Y+KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           +CHLK +SKH+AA AY +A  CYKK    EA SC+E+AVN+FC+IGRLNMAARYYKEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
            +ES+Q  EQAI YFEKAA+ FQNEEVTTSANQC  KVAQ+AAQLEQY+KAI IYE+IA+
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIAS 240
           +SL +NLLKYGVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTF+G+RE K LAD+AS
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLADLAS 240

Query: 241 AIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           AIDEED+AKFTDVVKEFDSMT LD  KTT+LLRVK+KLKAKELEEDDLT
Sbjct: 241 AIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of CmaCh04G010850 vs. TAIR 10
Match: AT3G56190.2 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 341.7 bits (875), Expect = 5.9e-94
Identity = 174/239 (72.80%), Postives = 204/239 (85.36%), Query Frame = 0


Query: 53  GSTYIKLA-NCHLKM-ESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNM 112
           GS++  L+  C L + +SKH+AA AY +A  CYKK    EA SC+E+AVN+FC+IGRLNM
Sbjct: 2   GSSWKSLSKTCRLSLKDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNM 61

Query: 113 AARYYKEIAELFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQK 172
           AARYYKEIAE +ES+Q  EQAI YFEKAA+ FQNEEVTTSANQC  KVAQ+AAQLEQY+K
Sbjct: 62  AARYYKEIAEYYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEK 121

Query: 173 AIDIYEEIAKYSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSR 232
           AI IYE+IA++SL +NLLKYGVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTF+G+R
Sbjct: 122 AIKIYEDIARHSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTR 181

Query: 233 EYKLLADIASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 290
           E K LAD+ASAIDEED+AKFTDVVKEFDSMT LD  KTT+LLRVK+KLKAKELEEDDLT
Sbjct: 182 ECKFLADLASAIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 240

[Close]

BLAST of CmaCh04G010850 vs. TAIR 10
Match: AT3G56450.1 (alpha-soluble NSF attachment protein 1 )

HSP 1 Score: 237.7 bits (605), Expect = 1.2e-62
Identity = 135/253 (53.36%), Postives = 166/253 (65.61%), Query Frame = 0


Query: 11  FDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLANCHLKMESKH 70
           F  KAEKKL+    F +KY+DAADL +KA +SYKL+KSWD+AG  Y+KLA+CHLK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAELFESEQDIEQ 130
             A  Y+    C                                  EIAE +ES++  EQ
Sbjct: 159 -LANTYMIMDECMD-------------------------------HEIAEYYESDEMFEQ 218

Query: 131 AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAKYSLKSNLLKY 190
           AI Y+E AA+ FQ EEVTTSANQC  KVAQ+A+QLEQ  +     +  A++SL + LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSR---FMKTQARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLADIASAIDEEDVAKF 250
           GVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTFSG+RE K LAD+ASAIDEED+AKF
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLADLASAIDEEDIAKF 314

Query: 251 TDVVKEFDSMTTL 264
           TDV KE DS++ L
Sbjct: 339 TDVSKEIDSVSPL 314

The following BLAST results are available for this feature:

BLAST of CmaCh04G010850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SPE6	3.1e-124	75.43	Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2...	[more]
P93798	1.9e-121	74.74	Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
Q9M5P8	1.5e-113	69.90	Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9LXZ5	1.7e-61	53.36	Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1...	[more]
Q54NP6	1.4e-60	44.09	Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snp...	[more]

BLAST of CmaCh04G010850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JUA6	6.5e-157	100.00	alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1GYT0	1.4e-156	99.65	alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1CZQ2	3.7e-152	95.16	alpha-soluble NSF attachment protein 2-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1HIF0	3.1e-135	85.81	alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A7N2R9Y3	3.1e-135	84.43	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of CmaCh04G010850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022991930.1	1.3e-156	100.00	alpha-soluble NSF attachment protein 2-like [Cucurbita maxima]	[more]
XP_022956354.1	3.0e-156	99.65	alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023536437.1...	[more]
XP_038893428.1	2.8e-154	97.58	alpha-soluble NSF attachment protein 2-like [Benincasa hispida]	[more]
XP_022146873.1	7.6e-152	95.16	alpha-soluble NSF attachment protein 2-like [Momordica charantia]	[more]
KAF3964963.1	1.3e-135	84.78	hypothetical protein CMV_010801 [Castanea mollissima]	[more]

BLAST of CmaCh04G010850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56190.1	2.2e-125	75.43	alpha-soluble NSF attachment protein 2	[more]
AT3G56190.2	5.9e-94	72.80	alpha-soluble NSF attachment protein 2	[more]
AT3G56450.1	1.2e-62	53.36	alpha-soluble NSF attachment protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 151..178
None	No IPR available	PFAM	PF14938	SNAP	coord: 7..278 e-value: 3.9E-109 score: 364.5
None	No IPR available	PANTHER	PTHR13768:SF37	ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN	coord: 1..289
IPR000744	NSF attachment protein	PRINTS	PR00448	NSFATTACHMNT	coord: 119..136 score: 29.37 coord: 152..175 score: 71.43 coord: 183..202 score: 36.43 coord: 28..47 score: 39.29 coord: 255..275 score: 53.06 coord: 91..108 score: 34.92 coord: 224..244 score: 43.54
IPR000744	NSF attachment protein	PANTHER	PTHR13768	SOLUBLE NSF ATTACHMENT PROTEIN SNAP	coord: 1..289
IPR000744	NSF attachment protein	CDD	cd15832	SNAP	coord: 7..278 e-value: 1.56911E-123 score: 352.267
IPR019734	Tetratricopeptide repeat	SMART	SM00028	tpr_5	coord: 53..86 e-value: 190.0 score: 3.7 coord: 112..145 e-value: 0.0062 score: 25.7
IPR019734	Tetratricopeptide repeat	PROSITE	PS50005	TPR	coord: 112..145 score: 9.7649
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 6..288 e-value: 1.2E-107 score: 361.4
IPR011990	Tetratricopeptide-like helical domain superfamily	SUPERFAMILY	48452	TPR-like	coord: 7..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G010850.1	CmaCh04G010850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0035494	SNARE complex disassembly
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005483	soluble NSF attachment protein activity
molecular_function	GO:0019905	syntaxin binding
molecular_function	GO:0005515	protein binding