CmaCh04G009100 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G009100
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	TIR domain-containing protein-like
Location	Cma_Chr04: 4729153 .. 4729995 (+)
RNA-Seq Expression	CmaCh04G009100
Synteny	CmaCh04G009100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACCGTGTGGCTGCCGTCGCGAAAATCACGCCGAGACTGTTTCTAGCTCAGCTGGGGAGCAAGCAGTTGCAGAGGGGTATTGCCAAACCCTGCGATGTGTTCATAAACCACAGAGGCGTCGACACCAAGAAGACAGTGGCGGCGTTGCTGTACGACAGGCTCATCCGGGGCAACTTCCGTCCGTTTCTTGATTACAGAAATTTGAAGCCTGGGGAGAAGCTGTTCGACGAACTCCACGGCGCCATTAGACAGTGTAAAGTGGGCGTGGCTGTGTTTTCGCCTCGGTATTGCGAGTCTTATTTCTGCCTCCACGAGTTGGCTATGATCATCGAGTCCAACAAGAAGGTACCCCAAATGGGTTTTCTTTGAATGTTTACAATTAGAATTCAACAACATCATCAAATGAATTGAATTATTGAACAGGTGATTCCTATATTTTGTGATGTGAACCCATCTCAGCTTCGGATAAAGGAGGACGATGGGTACGAAACAGAGGATCTGAGAAGGTTCAATTGGGCTCTTGAACAAGCTAACCAGTTGGAGGGAATCAAATTTAATTCCTCAAAACAGTAAGTAACAAAGATGTGGGCTAATCATCCTACAAAATTAATGTTTATATCCCTCTAATTAACCTAATTCTGTTGTTTCAGGACCTTATCAGACATTGTGACAAGGGCTTCAAATATTGTGATCAGTAGTTTGGTTGAGATGGACGGTAGGGATGAACAGTAGGCCCAACATCCAACACTTTATTCTTATTCTTTTATACATCATTCAATTTGTTAATTATTTCCTCTTCTCTCCAACACACAAAATGTAGCATGATTATGCATAATAACA

mRNA sequence

ATGAACCGTGTGGCTGCCGTCGCGAAAATCACGCCGAGACTGTTTCTAGCTCAGCTGGGGAGCAAGCAGTTGCAGAGGGGTATTGCCAAACCCTGCGATGTGTTCATAAACCACAGAGGCGTCGACACCAAGAAGACAGTGGCGGCGTTGCTGTACGACAGGCTCATCCGGGGCAACTTCCGTCCGTTTCTTGATTACAGAAATTTGAAGCCTGGGGAGAAGCTGTTCGACGAACTCCACGGCGCCATTAGACAGTGTAAAGTGGGCGTGGCTGTGTTTTCGCCTCGGTATTGCGAGTCTTATTTCTGCCTCCACGAGTTGGCTATGATCATCGAGTCCAACAAGAAGGTGATTCCTATATTTTGTGATGTGAACCCATCTCAGCTTCGGATAAAGGAGGACGATGGGTACGAAACAGAGGATCTGAGAAGGTTCAATTGGGCTCTTGAACAAGCTAACCAGTTGGAGGGAATCAAATTTAATTCCTCAAAACAGACCTTATCAGACATTGTGACAAGGGCTTCAAATATTGTGATCAGTAGTTTGGTTGAGATGGACGGTAGGGATGAACAGTAGGCCCAACATCCAACACTTTATTCTTATTCTTTTATACATCATTCAATTTGTTAATTATTTCCTCTTCTCTCCAACACACAAAATGTAGCATGATTATGCATAATAACA

Coding sequence (CDS)

ATGAACCGTGTGGCTGCCGTCGCGAAAATCACGCCGAGACTGTTTCTAGCTCAGCTGGGGAGCAAGCAGTTGCAGAGGGGTATTGCCAAACCCTGCGATGTGTTCATAAACCACAGAGGCGTCGACACCAAGAAGACAGTGGCGGCGTTGCTGTACGACAGGCTCATCCGGGGCAACTTCCGTCCGTTTCTTGATTACAGAAATTTGAAGCCTGGGGAGAAGCTGTTCGACGAACTCCACGGCGCCATTAGACAGTGTAAAGTGGGCGTGGCTGTGTTTTCGCCTCGGTATTGCGAGTCTTATTTCTGCCTCCACGAGTTGGCTATGATCATCGAGTCCAACAAGAAGGTGATTCCTATATTTTGTGATGTGAACCCATCTCAGCTTCGGATAAAGGAGGACGATGGGTACGAAACAGAGGATCTGAGAAGGTTCAATTGGGCTCTTGAACAAGCTAACCAGTTGGAGGGAATCAAATTTAATTCCTCAAAACAGACCTTATCAGACATTGTGACAAGGGCTTCAAATATTGTGATCAGTAGTTTGGTTGAGATGGACGGTAGGGATGAACAGTAG

Protein sequence

MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPIFCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVISSLVEMDGRDEQ

Homology

[Close]

BLAST of CmaCh04G009100 vs. ExPASy Swiss-Prot
Match: I1GTC2 (TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 7.3e-37
Identity = 72/158 (45.57%), Postives = 110/158 (69.62%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           +VFINHRGVDTK+TVA LLYDRL +   R FLD  +++PG++L + +  AIR+C V VA+
Sbjct: 54  EVFINHRGVDTKRTVARLLYDRLAQAGLRGFLDNMSMRPGDRLEERIGSAIRECTVAVAI 113

Query: 93  FSPRYCESYFCLHELAMIIESNKKVIPIFCDVNPSQL----RIKEDDGYETEDLRRFNWA 152
           FSP YC+S +CL ELAM++ES K +IPIF D+ PS L     + + + Y   DL RF +A
Sbjct: 114 FSPSYCDSEYCLRELAMLVESRKAIIPIFYDIKPSDLLLPQAVADSEVYLPRDLERFKFA 173

Query: 153 LEQANQLEGIKFNSSKQTLSDIVTRASNIVISSLVEMD 187
           L +A    GI ++S+   ++++V+ A++ V+ ++ +M+
Sbjct: 174 LREAKHTVGITYDSATGDMAELVSAAADAVMYNMEKME 211

[Close]

BLAST of CmaCh04G009100 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.1e-15
Identity = 42/102 (41.18%), Postives = 61/102 (59.80%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           DVF++ RG DT+KT    L++ LI      F+D + LK G+ +  EL  AI + +  V V
Sbjct: 13  DVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVV 72

Query: 93  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDVNPSQLR 131
           FS  Y  S +CL EL  I+E ++K    V+P+F DV+PS +R
Sbjct: 73  FSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVR 114

[Close]

BLAST of CmaCh04G009100 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.0e-14
Identity = 59/180 (32.78%), Postives = 92/180 (51.11%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           DVF++ RG DT+KT  + LY+ L     + F D + L+ G  +  EL  AI + +  + V
Sbjct: 13  DVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAIVV 72

Query: 93  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDVNPSQLRIKED------DGYET--- 152
           FS  Y  S +CL+EL  I+E   +    VIPIF DV+PS +R +++      + +ET   
Sbjct: 73  FSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHETKYK 132

Query: 153 ---EDLRRFNWALEQANQLEGIKFNSSK-------QTLSDIVTRASNIVISSLVEMDGRD 190
              E ++R+  AL +A  L+G   N  K       Q +  I ++   I +S L  + G D
Sbjct: 133 DDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKISLSYLQNIVGID 192

[Close]

BLAST of CmaCh04G009100 vs. ExPASy Swiss-Prot
Match: Q9SSN3 (Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.8e-12
Identity = 38/102 (37.25%), Postives = 55/102 (53.92%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           DVF++ RG DT+    + LY  L+R + R F D + L+ G++   EL   I   +  V V
Sbjct: 10  DVFLSFRGHDTRHNFISFLYKELVRRSIRTFKDDKELENGQRFSPELKSPIEVSRFAVVV 69

Query: 93  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDVNPSQLR 131
            S  Y  S +CL EL  I++  KK    V+PIF  V P+ +R
Sbjct: 70  VSENYAASSWCLDELVTIMDFEKKGSITVMPIFYGVEPNHVR 111

[Close]

BLAST of CmaCh04G009100 vs. ExPASy Swiss-Prot
Match: F4JNB7 (Disease resistance protein RPP5 OS=Arabidopsis thaliana OX=3702 GN=RPP5 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.6e-10
Identity = 35/102 (34.31%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           DVF +  GVD +KT  + L + L   +   F+D+  ++    +  EL  AIR+ ++ + +
Sbjct: 13  DVFPSFSGVDVRKTFLSHLIEALDGKSINTFIDH-GIERSRTIAPELISAIREARISIVI 72

Query: 93  FSPRYCESYFCLHELAMI----IESNKKVIPIFCDVNPSQLR 131
           FS  Y  S +CL+EL  I     +  + VIP+F DV+PS++R
Sbjct: 73  FSKNYASSTWCLNELVEIHKCFNDLGQMVIPVFYDVDPSEVR 113

[Close]

BLAST of CmaCh04G009100 vs. ExPASy TrEMBL
Match: A0A6J1KUS0 (TMV resistance protein N-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496557 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.5e-104
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
           FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS
Sbjct: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 191

[Close]

BLAST of CmaCh04G009100 vs. ExPASy TrEMBL
Match: A0A6J1FX23 (TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447771 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.0e-102
Identity = 187/191 (97.91%), Postives = 189/191 (98.95%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
           FCDVNPSQL IKE+DGYETEDLRRFNWALEQA QLEGIKFNSSKQTLSD+VTRASNIVIS
Sbjct: 121 FCDVNPSQLGIKEEDGYETEDLRRFNWALEQAKQLEGIKFNSSKQTLSDVVTRASNIVIS 180

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 191

[Close]

BLAST of CmaCh04G009100 vs. ExPASy TrEMBL
Match: A0A6J1KUX9 (uncharacterized protein LOC111496557 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496557 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.8e-94
Identity = 179/191 (93.72%), Postives = 179/191 (93.72%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKK    
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKK---- 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
                   LRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS
Sbjct: 121 --------LRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 179

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 179

[Close]

BLAST of CmaCh04G009100 vs. ExPASy TrEMBL
Match: A0A6J1FQ57 (uncharacterized protein LOC111447771 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447771 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 8.3e-92
Identity = 175/191 (91.62%), Postives = 177/191 (92.67%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKK    
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKK---- 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
                   L IKE+DGYETEDLRRFNWALEQA QLEGIKFNSSKQTLSD+VTRASNIVIS
Sbjct: 121 --------LGIKEEDGYETEDLRRFNWALEQAKQLEGIKFNSSKQTLSDVVTRASNIVIS 179

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 179

[Close]

BLAST of CmaCh04G009100 vs. ExPASy TrEMBL
Match: A0A6J1CP46 (disease resistance protein LAZ5 OS=Momordica charantia OX=3673 GN=LOC111012886 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.8e-75
Identity = 145/188 (77.13%), Postives = 163/188 (86.70%), Query Frame = 0


Query: 1   MNRV-AAVAKITPRLFLAQLGSKQL-QRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRG 60
           MNRV  AV KITP  FLAQLG   L  + I KPCDVFINHRGVDTKKT+AALLYDRL+R 
Sbjct: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60

Query: 61  NFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120
           N RPFLDYRNLKPGEKLFDE+HGAI QCKVGVAVFSPRYCESYFCLHELAMI+ES KKVI
Sbjct: 61  NLRPFLDYRNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESKKKVI 120

Query: 121 PIFCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIV 180
           PIFCDV PSQLRI++ + Y +++LRRFNWALE+A Q  G+KF+SSK+TLS++VT+ASNIV
Sbjct: 121 PIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKETLSEVVTKASNIV 180

Query: 181 ISSLVEMD 187
           I SL EMD
Sbjct: 181 IRSLDEMD 188

[Close]

BLAST of CmaCh04G009100 vs. NCBI nr
Match: XP_023002843.1 (TMV resistance protein N-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 387.9 bits (995), Expect = 5.1e-104
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
           FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS
Sbjct: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 191

[Close]

BLAST of CmaCh04G009100 vs. NCBI nr
Match: KAG6600735.1 (TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031376.1 TMV resistance protein N, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 381.3 bits (978), Expect = 4.8e-102
Identity = 188/191 (98.43%), Postives = 189/191 (98.95%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
           FCDVNPSQL IKE+DGYETEDLRRFNWALEQA QLEGIKFNSSKQTLSDIVTRASNIVIS
Sbjct: 121 FCDVNPSQLGIKEEDGYETEDLRRFNWALEQAKQLEGIKFNSSKQTLSDIVTRASNIVIS 180

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 191

[Close]

BLAST of CmaCh04G009100 vs. NCBI nr
Match: XP_022942870.1 (TMV resistance protein N-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 380.9 bits (977), Expect = 6.2e-102
Identity = 187/191 (97.91%), Postives = 189/191 (98.95%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
           FCDVNPSQL IKE+DGYETEDLRRFNWALEQA QLEGIKFNSSKQTLSD+VTRASNIVIS
Sbjct: 121 FCDVNPSQLGIKEEDGYETEDLRRFNWALEQAKQLEGIKFNSSKQTLSDVVTRASNIVIS 180

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 191

[Close]

BLAST of CmaCh04G009100 vs. NCBI nr
Match: XP_023540142.1 (TMV resistance protein N-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.2 bits (975), Expect = 1.1e-101
Identity = 187/191 (97.91%), Postives = 189/191 (98.95%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
           FCDVNPSQL IKE+DGYETEDLRRFNWALEQA QLEGIKFNSSKQTLSDIVTRASNIVI+
Sbjct: 121 FCDVNPSQLGIKEEDGYETEDLRRFNWALEQAKQLEGIKFNSSKQTLSDIVTRASNIVIT 180

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 191

[Close]

BLAST of CmaCh04G009100 vs. NCBI nr
Match: XP_023002903.1 (uncharacterized protein LOC111496557 isoform X2 [Cucurbita maxima])

HSP 1 Score: 353.2 bits (905), Expect = 1.4e-93
Identity = 179/191 (93.72%), Postives = 179/191 (93.72%), Query Frame = 0


Query: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60
           MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120
           RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKK    
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKK---- 120

Query: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180
                   LRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS
Sbjct: 121 --------LRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 179

Query: 181 SLVEMDGRDEQ 192
           SLVEMDGRDEQ
Sbjct: 181 SLVEMDGRDEQ 179

[Close]

BLAST of CmaCh04G009100 vs. TAIR 10
Match: AT1G52900.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-49
Identity = 96/186 (51.61%), Postives = 130/186 (69.89%), Query Frame = 0


Query: 9   KITPRLFLAQLGSKQLQRGIAKP---CDVFINHRGVDTKKTVAALLYDRLIRGNFRPFLD 68
           ++  +LFL  L S       AKP    DVFINHRG DTK+ +A LLYD L   N RPFLD
Sbjct: 16  QLNNKLFLDLLSSS----SSAKPKILHDVFINHRGSDTKRNIATLLYDNLKSRNLRPFLD 75

Query: 69  YRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPIFCDVN 128
            +N+KPG+KLFD ++ AI   KV V VFSP YC+SYFCLHELA+I+ES K+VIPIFCD+ 
Sbjct: 76  SKNMKPGDKLFDHINNAILTSKVAVTVFSPNYCDSYFCLHELALIMESRKRVIPIFCDIK 135

Query: 129 PSQLRIK-EDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVISSLVE 188
           PSQL +  E      ++++RF WAL++A  + G+ F+S K  LS++VT AS++++  LVE
Sbjct: 136 PSQLDVMIERVTCSDDEIQRFRWALQEAKDIVGLTFDSYKGNLSEVVTVASDVIVERLVE 195

Query: 189 MDGRDE 191
           ++ +DE
Sbjct: 196 LEAKDE 197

[Close]

BLAST of CmaCh04G009100 vs. TAIR 10
Match: AT1G61105.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 183.7 bits (465), Expect = 1.4e-46
Identity = 82/158 (51.90%), Postives = 116/158 (73.42%), Query Frame = 0


Query: 31  PCDVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGV 90
           PCD+FINHRG+DTKKT++ LLYD   R     FLD ++LKPG++LF E+  AI+ C VG+
Sbjct: 28  PCDIFINHRGIDTKKTISGLLYDCFTRQRLTAFLDSKSLKPGDRLFVEIDMAIKACGVGI 87

Query: 91  AVFSPRYCESYFCLHELAMIIESNKKVIPIFCDVNPSQLRIKEDDGYETEDLRRFNWALE 150
           AVFSPRYC+SYFCLHELA+++++ K+VIPIFCDV PS+L +K+D      ++RRF  ALE
Sbjct: 88  AVFSPRYCDSYFCLHELALLVKNKKRVIPIFCDVKPSELCVKDDKTRPAAEIRRFQLALE 147

Query: 151 QANQLEGIKFNSSKQTLSDIVTRASNIVISSLVEMDGR 189
           +A    G+ F++S    S+ +  AS+ V  +L+E++ R
Sbjct: 148 EAKYTVGLTFDTSNGDWSEFLAMASDAVTKNLLEVEER 185

[Close]

BLAST of CmaCh04G009100 vs. TAIR 10
Match: AT1G72950.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 79.3 bits (194), Expect = 3.6e-15
Identity = 54/174 (31.03%), Postives = 84/174 (48.28%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           DVF++ RG+DT+++  + LY  LIR N R F D + LK G ++  EL  AI   +  V V
Sbjct: 12  DVFLSFRGLDTRRSFISFLYKELIRRNIRTFKDDKELKNGRRITPELIRAIEGSRFAVVV 71

Query: 93  FSPRYCESYFCLHELAMIIE----SNKKVIPIFCDVNPSQLR---------IKEDDGYET 152
            S  Y  S +CL EL  I++     + KV+PIF  V+P  +R          K+ +G E 
Sbjct: 72  VSVNYAASRWCLEELVKIMDFENMGSLKVMPIFYGVDPCHVRRQIGEVAEQFKKHEGRED 131

Query: 153 -EDLRRFNWALEQANQLEG---IKFNSSKQTLSDIVTRASNIVISSLVEMDGRD 190
            E +  +  AL     + G    K+    + + +I  R S  ++      +G D
Sbjct: 132 HEKVLSWRQALTNLASISGDCSWKWEDDSKMVEEITDRISKELMIDTTRSNGSD 185

[Close]

BLAST of CmaCh04G009100 vs. TAIR 10
Match: AT1G72920.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 79.3 bits (194), Expect = 3.6e-15
Identity = 51/175 (29.14%), Postives = 82/175 (46.86%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           DVF++ RG+DT++   + LY  L+R   R F D + L+ G ++  EL  AI + K  V V
Sbjct: 10  DVFLSFRGLDTRRNFISFLYKELVRRKIRTFKDDKELENGRRISPELKRAIEESKFAVVV 69

Query: 93  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDVNPSQLR--------------IKED 152
            S  Y  S +CL EL  I++   K    V+PIF  V+P  LR               +E+
Sbjct: 70  VSVNYAASPWCLDELVKIMDFENKGSITVMPIFYGVDPCHLRRQIGDVAEQFKKHEAREE 129

Query: 153 DGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVISSLVEMDGRD 190
           D  +    RR   +L   +    +K+      + +I  + S  +++     +GRD
Sbjct: 130 DHEKVASWRRALTSLASISGECSLKWEDEANLVDEIADKISKNLMTVTTISNGRD 184

[Close]

BLAST of CmaCh04G009100 vs. TAIR 10
Match: AT1G17615.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 79.0 bits (193), Expect = 4.7e-15
Identity = 40/102 (39.22%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 33  DVFINHRGVDTKKTVAALLYDRLIRGNFRPFLDYRNLKPGEKLFDELHGAIRQCKVGVAV 92
           DVF++ RG DT++T  + LY  LI    R F D   L+ G ++  +L  AI   K+ V +
Sbjct: 16  DVFLSFRGKDTRRTFISFLYKELIEMGIRTFKDDVELQSGRRIASDLLAAIENSKIAVVI 75

Query: 93  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDVNPSQLR 131
            S  Y  S +CL EL MI++  KK    V+PIF +V P+ +R
Sbjct: 76  ISKNYSASPWCLQELVMIMDVEKKGSIIVMPIFYNVEPAHVR 117

The following BLAST results are available for this feature:

BLAST of CmaCh04G009100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1GTC2	7.3e-37	45.57	TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=...	[more]
A0A290U7C4	2.1e-15	41.18	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	1.0e-14	32.78	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
Q9SSN3	4.8e-12	37.25	Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR ...	[more]
F4JNB7	2.6e-10	34.31	Disease resistance protein RPP5 OS=Arabidopsis thaliana OX=3702 GN=RPP5 PE=1 SV=...	[more]

BLAST of CmaCh04G009100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KUS0	2.5e-104	100.00	TMV resistance protein N-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1FX23	3.0e-102	97.91	TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1KUX9	6.8e-94	93.72	uncharacterized protein LOC111496557 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FQ57	8.3e-92	91.62	uncharacterized protein LOC111447771 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1CP46	1.8e-75	77.13	disease resistance protein LAZ5 OS=Momordica charantia OX=3673 GN=LOC111012886 P...	[more]

BLAST of CmaCh04G009100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002843.1	5.1e-104	100.00	TMV resistance protein N-like isoform X1 [Cucurbita maxima]	[more]
KAG6600735.1	4.8e-102	98.43	TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031376.1 ...	[more]
XP_022942870.1	6.2e-102	97.91	TMV resistance protein N-like isoform X1 [Cucurbita moschata]	[more]
XP_023540142.1	1.1e-101	97.91	TMV resistance protein N-like [Cucurbita pepo subsp. pepo]	[more]
XP_023002903.1	1.4e-93	93.72	uncharacterized protein LOC111496557 isoform X2 [Cucurbita maxima]	[more]

BLAST of CmaCh04G009100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52900.1	1.3e-49	51.61	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G61105.1	1.4e-46	51.90	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G72950.1	3.6e-15	31.03	Disease resistance protein (TIR-NBS class)	[more]
AT1G72920.1	3.6e-15	29.14	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G17615.1	4.7e-15	39.22	Disease resistance protein (TIR-NBS class)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 31..177 e-value: 7.3E-17 score: 72.0
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 33..130 e-value: 2.5E-20 score: 73.0
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 30..178 score: 27.054169
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 29..182 e-value: 2.7E-33 score: 117.1
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 30..154
None	No IPR available	PANTHER	PTHR31008	COP1-INTERACTING PROTEIN-RELATED	coord: 28..187
None	No IPR available	PANTHER	PTHR31008:SF16	TOLL-INTERLEUKIN-RESISTANCE (TIR) DOMAIN FAMILY PROTEIN	coord: 28..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G009100.1	CmaCh04G009100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003953	NAD+ nucleosidase activity