CmaCh04G008970 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh04G008970 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 1.4e-259
Identity = 442/598 (73.91%), Postives = 513/598 (85.79%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAEVAIGSIG GYD+  DLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SI NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++LR+HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 FKMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESRE 240
            KMGGKDVIY KQQHSS L+P ++QKRLK++ADKRF E    +  GSE +   +K E++E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 QHLQFSDASSAIS-ALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITS 300
           Q L+F+D SS  S A KED + + KRRGG+D  NL H+EWLQTVQ+EPDVIS+SFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ++ AS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPN 420
           LQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPK +QL +D N
Sbjct: 361 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 420

Query: 421 NNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFF 480
            +  QES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V +HG +NVLFLRL F
Sbjct: 421 RSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLRLCF 480

Query: 481 CSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPP 540
             V+GAT+VK +EW+ + G A KSGLISTLISHHF+A QKPPP+PADVNINSA+YPGGPP
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDE 597
           VP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKGKISL+VK+SL T +L DE
Sbjct: 541 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDE 598

BLAST of CmaCh04G008970 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 3.2e-171
Identity = 322/600 (53.67%), Postives = 413/600 (68.83%), Query Frame = 0

Query: 10  AAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGISISNVPKSIK 69
           AAE A+  IG GYD+ +D+R   CK    GSRL+EID     +++ PGGI ++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAF 129
           CDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++ K LA+
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 DGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGFKMGGKDVIY 189
           DG FI+LY+V + + Q+ LRD V++ VPSSWD AALA FIE +GTH++VG  MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSSTLEPSEVQKRLKDMADKRF----TETMGQYGMGSEWIYNDNKFESREQHLQFS 249
           +KQ   S  EP E+QK LK   D+RF     E+     + S     +N  +   Q    S
Sbjct: 194 VKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTS 253

Query: 250 DASSAISALK-EDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVP 309
            +S+ +   K E+I+ +  RRGG D +   H  WL TV   P+VIS+ F+PITSLL+G+P
Sbjct: 254 VSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLSGLP 313

Query: 310 GSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQFSLM 369
           G+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R +++  SLQFSLM
Sbjct: 314 GTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLM 373

Query: 370 GSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNNSGQE 429
           G KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P S  L+ D      +E
Sbjct: 374 GPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEE 433

Query: 430 SYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNHGMRNVLFLRLFFC 489
             ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLFLRL F 
Sbjct: 434 PVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRLGF- 493

Query: 490 SVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPP---KP-ADVNINSAVYPG 549
           S+  + V +++ W+     + KSG+ S +IS   S    P P   KP + ++INSAVYP 
Sbjct: 494 SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAVYPR 553

Query: 550 GPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSD 596
           GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE GKIS++ K+SLLTV+  D
Sbjct: 554 GPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVISED 609

BLAST of CmaCh04G008970 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 5.0e-132
Identity = 265/613 (43.23%), Postives = 375/613 (61.17%), Query Frame = 0

Query: 12  EVAIGSIGRGYDITTDLRLKYCK---GNFHGSRLIEIDEGVGHEIVLPGGISISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGFKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G  +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLEPSEVQKRLKDMADKRFTET--MGQYGMGSEWIYNDNKFESREQHLQF 251
           V+ ++Q  SS L+   ++  L D+ D+ FT +  +    +   + ++ ++ +  E    F
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 249

Query: 252 SD----ASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 311
            D    A +  S   ++ +T+   + G D     H EWL TV  +PD I+ +FIPITSLL
Sbjct: 250 DDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLL 309

Query: 312 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRN-LASL 371
             VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      +   +L
Sbjct: 310 KDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPAL 369

Query: 372 QFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSF--QLTEDP 431
             + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +   +   ++T++ 
Sbjct: 370 HINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEH 429

Query: 432 N-NNSGQESYDRRYLEKVQSKYFSHVCTAPVEADED--------------LSIVTGAQLQ 491
               S Q + + RY E +  K FSHVCT PV+ D +                IVTGAQL+
Sbjct: 430 IWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLE 489

Query: 492 VVNHGMRNVLFLRLFFCSVLGATVVKQAEWEGSPGLAAKSGLISTLI------SHHFSAV 551
           V  HG ++VL LRL +  V    VV+ +   G  G + KSG+ S++       S H + +
Sbjct: 490 VKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMI 549

Query: 552 QKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVE 590
           QK      +V ++S V+PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G RL ++
Sbjct: 550 QK---DKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLD 609

BLAST of CmaCh04G008970 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.5e-128
Identity = 269/596 (45.13%), Postives = 353/596 (59.23%), Query Frame = 0

Query: 14  AIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGISISNVPKSIKCDKG 73
           AI ++GRG+D+T+D+RL YCKG   GSRL+ I+EG   ++ L  G  + NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGFKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+    +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQHLQFSDASSAISA 253
            SS L  SE++  + DM   RF E   Q   G                L++ D       
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD------- 263

Query: 254 LKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVPGSGFLSHAI 313
             +DI  I++RRGG D +   H  W +TV   PD+I+++F PI SLL GVPG   L+ AI
Sbjct: 264 --KDITVIFRRRGGDD-LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAI 323

Query: 314 NLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQFSLMGSKLYVNTT 373
            LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++  
Sbjct: 324 ELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISAD 383

Query: 374 PVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPN------NNSGQESYD 433
            V VGR+PVTGLRL LEG + N LSIHLQHL SLPK  Q   D +         G E  D
Sbjct: 384 QVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQD 443

Query: 434 RRYLEKVQSKYFSHVCTAPVEADE----DLS---IVTGAQLQVVNHGMRNVLFLRLFFCS 493
            R+ E ++ K FSHV T+P+E  E    DLS   IVTGAQL V + G +NVL L+L F  
Sbjct: 444 SRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSK 503

Query: 494 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP--- 553
           V G T ++++ W+ +P   A SG +                            PGGP   
Sbjct: 504 VPGCT-IRRSVWDHTP--VASSGRLE---------------------------PGGPSTS 559

Query: 554 ----PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLL 590
                V  Q+ KL K VD++EM +GPQ+ PG+W+V+GA+L VEKGKI LRVK+SLL
Sbjct: 564 SSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of CmaCh04G008970 vs. ExPASy TrEMBL
Match: A0A6J1J8T9 (MACPF domain-containing protein At4g24290 OS=Cucurbita maxima OX=3661 GN=LOC111482322 PE=4 SV=1)

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 602/602 (100.00%), Postives = 602/602 (100.00%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180

Query: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240
           KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240

Query: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420

Query: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480
           SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600

Query: 601 DN 603
           DN
Sbjct: 601 DN 602

BLAST of CmaCh04G008970 vs. ExPASy TrEMBL
Match: A0A6J1FQI8 (MACPF domain-containing protein At4g24290 OS=Cucurbita moschata OX=3662 GN=LOC111447540 PE=4 SV=1)

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 591/602 (98.17%), Postives = 597/602 (99.17%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFH SRLIEIDEGVGH IVLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240
           KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGM SEWIYN++KFESREQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMSSEWIYNNDKFESREQ 240

Query: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HLQFSDASSAISALKEDI+TIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLP SFQLTEDPN+N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPISFQLTEDPNSN 420

Query: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480
           SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+LSDEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEIN 600

Query: 601 DN 603
           DN
Sbjct: 601 DN 602

BLAST of CmaCh04G008970 vs. ExPASy TrEMBL
Match: A0A5D3BT29 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00720 PE=4 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 557/605 (92.07%), Postives = 574/605 (94.88%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN HGSRLIEIDEGVGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFES 240
           KMGGKDVIYMKQQHSSTL   EPSEVQK LK+MADKRF+ET+ QYGMGSE +YN++K E 
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300
           REQHL+F DAS+AIS LK DI+ I KRRGGSDRI+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLRFGDASTAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPK FQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420

Query: 421 NNNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLF 480
           N N  Q+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVN GMRNVLFLRL 
Sbjct: 421 NGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLR 480

Query: 481 FCSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540
           F SV GATVV+QAEWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FSSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEE 600
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+L DEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600

Query: 601 EINDN 603
           EINDN
Sbjct: 601 EINDN 604

BLAST of CmaCh04G008970 vs. ExPASy TrEMBL
Match: A0A1S3BTG2 (MACPF domain-containing protein At4g24290 OS=Cucumis melo OX=3656 GN=LOC103493321 PE=4 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 557/605 (92.07%), Postives = 574/605 (94.88%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN HGSRLIEIDEGVGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFES 240
           KMGGKDVIYMKQQHSSTL   EPSEVQK LK+MADKRF+ET+ QYGMGSE +YN++K E 
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300
           REQHL+F DAS+AIS LK DI+ I KRRGGSDRI+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLRFGDASTAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPK FQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420

Query: 421 NNNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLF 480
           N N  Q+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVN GMRNVLFLRL 
Sbjct: 421 NGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLR 480

Query: 481 FCSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540
           F SV GATVV+QAEWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FSSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEE 600
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+L DEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600

Query: 601 EINDN 603
           EINDN
Sbjct: 601 EINDN 604

BLAST of CmaCh04G008970 vs. ExPASy TrEMBL
Match: A0A6J1EMJ3 (MACPF domain-containing protein At4g24290-like OS=Cucurbita moschata OX=3662 GN=LOC111435883 PE=4 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 544/602 (90.37%), Postives = 559/602 (92.86%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGH NTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHLNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPS+WDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSTWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240
           KMGGKDVIYMKQQH STL+PSEVQKRLK+MADKRF+ET+GQYGMGSE IYN +K      
Sbjct: 181 KMGGKDVIYMKQQHYSTLQPSEVQKRLKEMADKRFSETIGQYGMGSEQIYNSDK------ 240

Query: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
                           DIL IYKRRGGSDRIN PHHEWLQTVQLEPDVIS+SFIPITSLL
Sbjct: 241 ----------------DILAIYKRRGGSDRINYPHHEWLQTVQLEPDVISMSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPK FQLTEDPN N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420

Query: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480
             QESYDRRYLEKVQSKYFSHVCTAP+EADEDLSIVTGAQLQVVNHGM+NVLFLRL F S
Sbjct: 421 FCQESYDRRYLEKVQSKYFSHVCTAPIEADEDLSIVTGAQLQVVNHGMKNVLFLRLRFSS 480

Query: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVV+Q EWEGSPGLAAKSGLISTLISHHFS VQKPPPKPADV INSAVYPGGPPVP
Sbjct: 481 VLGATVVRQPEWEGSPGLAAKSGLISTLISHHFSTVQKPPPKPADVIINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQ+TPGYWVVSGARLMVEKG+ISLRV+FSLLTV+L DEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQDTPGYWVVSGARLMVEKGRISLRVRFSLLTVILPDEEEIN 580

Query: 601 DN 603
           DN
Sbjct: 601 DN 580

BLAST of CmaCh04G008970 vs. NCBI nr
Match: XP_022983823.1 (MACPF domain-containing protein At4g24290 [Cucurbita maxima])

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 602/602 (100.00%), Postives = 602/602 (100.00%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180

Query: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240
           KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240

Query: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420

Query: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480
           SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600

Query: 601 DN 603
           DN
Sbjct: 601 DN 602

BLAST of CmaCh04G008970 vs. NCBI nr
Match: KAG7031360.1 (MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 593/602 (98.50%), Postives = 599/602 (99.50%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFH SRLIEIDEGVGH IVLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240
           KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYN++KFESREQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFESREQ 240

Query: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HLQFSDASSAISALKEDI+TIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPN+N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNSN 420

Query: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480
           SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+LSDEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEIN 600

Query: 601 DN 603
           DN
Sbjct: 601 DN 602

BLAST of CmaCh04G008970 vs. NCBI nr
Match: XP_022942532.1 (MACPF domain-containing protein At4g24290 [Cucurbita moschata])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 591/602 (98.17%), Postives = 597/602 (99.17%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFH SRLIEIDEGVGH IVLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240
           KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGM SEWIYN++KFESREQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMSSEWIYNNDKFESREQ 240

Query: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HLQFSDASSAISALKEDI+TIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLP SFQLTEDPN+N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPISFQLTEDPNSN 420

Query: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480
           SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+LSDEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEIN 600

Query: 601 DN 603
           DN
Sbjct: 601 DN 602

BLAST of CmaCh04G008970 vs. NCBI nr
Match: XP_023540812.1 (MACPF domain-containing protein At4g24290 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 591/602 (98.17%), Postives = 598/602 (99.34%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60
           MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNF+ SRLIEIDEGVGH IVLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFNDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240
           KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYN++KFESREQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFESREQ 240

Query: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HLQFSDASSAISALKEDI+TIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPN+N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNSN 420

Query: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480
           SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+LSDEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEIN 600

Query: 601 DN 603
           DN
Sbjct: 601 DN 602

BLAST of CmaCh04G008970 vs. NCBI nr
Match: KAG6600721.1 (MACPF domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 585/595 (98.32%), Postives = 592/595 (99.50%), Query Frame = 0

Query: 8   SKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGISISNVPKS 67
           S+AAEVAIGSIGRGYDITTDLRLKYCKGNFH SRLIEIDEGVGH IVLPGGISISNVPKS
Sbjct: 54  SRAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGISISNVPKS 113

Query: 68  IKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNL 127
           IKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNL
Sbjct: 114 IKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNL 173

Query: 128 AFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGFKMGGKDV 187
           AFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVG KMGGKDV
Sbjct: 174 AFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDV 233

Query: 188 IYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQHLQFSDA 247
           IYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYN++KFESREQHLQFSDA
Sbjct: 234 IYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFESREQHLQFSDA 293

Query: 248 SSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVPGSG 307
           SSAISALKEDI+TIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVPGSG
Sbjct: 294 SSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVPGSG 353

Query: 308 FLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQFSLMGSK 367
           FLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQFSLMGSK
Sbjct: 354 FLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQFSLMGSK 413

Query: 368 LYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNNSGQESYD 427
           LYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPN+NSGQESYD
Sbjct: 414 LYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNSNSGQESYD 473

Query: 428 RRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCSVLGATVV 487
           RRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCSVLGATVV
Sbjct: 474 RRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCSVLGATVV 533

Query: 488 KQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVPVQAPKLL 547
           KQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVPVQAPKLL
Sbjct: 534 KQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVPVQAPKLL 593

Query: 548 KFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEINDN 603
           KFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+LSDEEEINDN
Sbjct: 594 KFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEINDN 648

BLAST of CmaCh04G008970 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 896.7 bits (2316), Expect = 9.9e-261
Identity = 442/598 (73.91%), Postives = 513/598 (85.79%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAEVAIGSIG GYD+  DLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SI NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++LR+HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 FKMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESRE 240
            KMGGKDVIY KQQHSS L+P ++QKRLK++ADKRF E    +  GSE +   +K E++E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 QHLQFSDASSAIS-ALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITS 300
           Q L+F+D SS  S A KED + + KRRGG+D  NL H+EWLQTVQ+EPDVIS+SFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ++ AS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPN 420
           LQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPK +QL +D N
Sbjct: 361 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 420

Query: 421 NNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFF 480
            +  QES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V +HG +NVLFLRL F
Sbjct: 421 RSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLRLCF 480

Query: 481 CSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPP 540
             V+GAT+VK +EW+ + G A KSGLISTLISHHF+A QKPPP+PADVNINSA+YPGGPP
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDE 597
           VP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKGKISL+VK+SL T +L DE
Sbjct: 541 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDE 598

BLAST of CmaCh04G008970 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 603.2 bits (1554), Expect = 2.3e-172
Identity = 322/600 (53.67%), Postives = 413/600 (68.83%), Query Frame = 0

Query: 10  AAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGISISNVPKSIK 69
           AAE A+  IG GYD+ +D+R   CK    GSRL+EID     +++ PGGI ++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAF 129
           CDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++ K LA+
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 DGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGFKMGGKDVIY 189
           DG FI+LY+V + + Q+ LRD V++ VPSSWD AALA FIE +GTH++VG  MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSSTLEPSEVQKRLKDMADKRF----TETMGQYGMGSEWIYNDNKFESREQHLQFS 249
           +KQ   S  EP E+QK LK   D+RF     E+     + S     +N  +   Q    S
Sbjct: 194 VKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTS 253

Query: 250 DASSAISALK-EDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVP 309
            +S+ +   K E+I+ +  RRGG D +   H  WL TV   P+VIS+ F+PITSLL+G+P
Sbjct: 254 VSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLSGLP 313

Query: 310 GSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQFSLM 369
           G+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R +++  SLQFSLM
Sbjct: 314 GTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLM 373

Query: 370 GSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNNSGQE 429
           G KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P S  L+ D      +E
Sbjct: 374 GPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEE 433

Query: 430 SYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNHGMRNVLFLRLFFC 489
             ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLFLRL F 
Sbjct: 434 PVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRLGF- 493

Query: 490 SVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPP---KP-ADVNINSAVYPG 549
           S+  + V +++ W+     + KSG+ S +IS   S    P P   KP + ++INSAVYP 
Sbjct: 494 SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAVYPR 553

Query: 550 GPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSD 596
           GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE GKIS++ K+SLLTV+  D
Sbjct: 554 GPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVISED 609

BLAST of CmaCh04G008970 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 473.0 bits (1216), Expect = 3.5e-133
Identity = 265/613 (43.23%), Postives = 375/613 (61.17%), Query Frame = 0

Query: 12  EVAIGSIGRGYDITTDLRLKYCK---GNFHGSRLIEIDEGVGHEIVLPGGISISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGFKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G  +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLEPSEVQKRLKDMADKRFTET--MGQYGMGSEWIYNDNKFESREQHLQF 251
           V+ ++Q  SS L+   ++  L D+ D+ FT +  +    +   + ++ ++ +  E    F
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 249

Query: 252 SD----ASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 311
            D    A +  S   ++ +T+   + G D     H EWL TV  +PD I+ +FIPITSLL
Sbjct: 250 DDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLL 309

Query: 312 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRN-LASL 371
             VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      +   +L
Sbjct: 310 KDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPAL 369

Query: 372 QFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSF--QLTEDP 431
             + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +   +   ++T++ 
Sbjct: 370 HINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEH 429

Query: 432 N-NNSGQESYDRRYLEKVQSKYFSHVCTAPVEADED--------------LSIVTGAQLQ 491
               S Q + + RY E +  K FSHVCT PV+ D +                IVTGAQL+
Sbjct: 430 IWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLE 489

Query: 492 VVNHGMRNVLFLRLFFCSVLGATVVKQAEWEGSPGLAAKSGLISTLI------SHHFSAV 551
           V  HG ++VL LRL +  V    VV+ +   G  G + KSG+ S++       S H + +
Sbjct: 490 VKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMI 549

Query: 552 QKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVE 590
           QK      +V ++S V+PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G RL ++
Sbjct: 550 QK---DKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLD 609

BLAST of CmaCh04G008970 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 461.8 bits (1187), Expect = 8.2e-130
Identity = 235/335 (70.15%), Postives = 276/335 (82.39%), Query Frame = 0

Query: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAEVAIGSIG GYD+  DLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SI NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++LR+HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 FKMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESRE 240
            KMGGKDVIY KQQHSS L+P ++QKRLK++ADKRF E    +  GSE +   +K E++E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 QHLQFSDASSAIS-ALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITS 300
           Q L+F+D SS  S A KED + + KRRGG+D  NL H+EWLQTVQ+EPDVIS+SFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKP--PIEELHQFLE 332
           LLNGVPGSGFLSHAINLYLR KP   +  +H FLE
Sbjct: 301 LLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335

BLAST of CmaCh04G008970 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 461.5 bits (1186), Expect = 1.1e-129
Identity = 269/596 (45.13%), Postives = 353/596 (59.23%), Query Frame = 0

Query: 14  AIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGISISNVPKSIKCDKG 73
           AI ++GRG+D+T+D+RL YCKG   GSRL+ I+EG   ++ L  G  + NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGFKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+    +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQHLQFSDASSAISA 253
            SS L  SE++  + DM   RF E   Q   G                L++ D       
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD------- 263

Query: 254 LKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVPGSGFLSHAI 313
             +DI  I++RRGG D +   H  W +TV   PD+I+++F PI SLL GVPG   L+ AI
Sbjct: 264 --KDITVIFRRRGGDD-LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAI 323

Query: 314 NLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQFSLMGSKLYVNTT 373
            LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++  
Sbjct: 324 ELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISAD 383

Query: 374 PVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPN------NNSGQESYD 433
            V VGR+PVTGLRL LEG + N LSIHLQHL SLPK  Q   D +         G E  D
Sbjct: 384 QVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQD 443

Query: 434 RRYLEKVQSKYFSHVCTAPVEADE----DLS---IVTGAQLQVVNHGMRNVLFLRLFFCS 493
            R+ E ++ K FSHV T+P+E  E    DLS   IVTGAQL V + G +NVL L+L F  
Sbjct: 444 SRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSK 503

Query: 494 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP--- 553
           V G T ++++ W+ +P   A SG +                            PGGP   
Sbjct: 504 VPGCT-IRRSVWDHTP--VASSGRLE---------------------------PGGPSTS 559

Query: 554 ----PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLL 590
                V  Q+ KL K VD++EM +GPQ+ PG+W+V+GA+L VEKGKI LRVK+SLL
Sbjct: 564 SSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559