CmaCh04G008850 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G008850
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein NRT1/ PTR FAMILY 4.3-like
LocationCma_Chr04: 4598443 .. 4601568 (-)
RNA-Seq ExpressionCmaCh04G008850
SyntenyCmaCh04G008850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TACCAAATGGAAATGGAAATGGAAATGGACATTGAGAGAAGGAGAGACACCCATCTCATGCCTTCTGAGGATCTCACTGTTGATTGGAGAGGAAGACCCTCCAACCCCGTCGCCCACGGCGGGATGAGAGCCGCTGCTTTTGTTCTCGGTAACGACCCTCTTTCTCTCTTGACTCAAATTCTTAAGTGTTGCTGACGACAATGAATAGAAATAGGGTATGAAAATAGAAAAGTTCTCGTCTCTCTTATTTTTTTATATTGATTCTGGATTTTTTTTTAAAATGGTTCCAAAAACTTTTTTCGAAATGGGTTTTAAGGAGAGAAAAATAAGCACGATAAAAGATCATAGTTTTAAATCTCGATCTCGTAAAATTTAACTTGTAATACTTTTGAATCTGATAGAGCGATCGCTTTGTATACGGTCCGTGCAGGGCTGCAATCATTCGAGATAATGGCGATTGCTGCAGTTGGGAACAATCTGATAACATATGTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACGTAGTGACAAACTTTGTTGGGACTGTGTTTCTTTTGGCACTTCTTGGCGGTTTCCTTTCTGATTCCTATCTTGGGAGTTTTTGGACAATGCTTATCTTTGGCTTTGTTGAACTTTCTGTAAGTTCAGTTCCCCCTCCCTTCCATCGTGTTTGTTTATTGTACACATTTTTTATGTGGTTTGAATCCAAAACATCTTATCTCTCAATCGATGATATATAATTTTATTAGTTGAGTTATATTTAGGTTCACCTATGATAGCTGACATATATAATTTCGAAAAAATACAGCTTTTGCATGTGCATGTATACACAATAGATTAGATAAGAAAAACGGTATTATTTAAATGCTAAAAATTGTGTTGTTTTAGGGGTTCATATTACTATCAGTTCAGGCTCATCTTCCCCAACTAAAGCCACCCCAATGCAACATGCTAAGTGAAGGAGATGACTGCGTGGAAGCCAAGGGGATAAAGGCATTGATATTCTTTGTGGCACTCTACTTGGTGGCTCTTGGGAGTGGGTGTGTGAAGCCCAACATGATAGCTCATGGGGCTGATCAGTTCACTCCACAAGATCCAAGCCAATCCAAGAGGCTTTCAACATACTTCAATGCTGCTTATTTTGCCTTCTCAATTGGAGAGCTCATTGCCCTCACTCTTGTTGTTTGGATCCAAACCCATTCTGGGATGGACGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCTATGGGGTTGATCAGCTTGGTTGGCGGTATTTTTTACTACAGGAATAAACCCCCTCAAGGAGCCCTTTTTATCCCAATTCTTCAAGTAATTTTCTTGCTTTCTTTCTTTCTTTACAACTTTACATGTGTTAGTATTGTGCACACTTAACATGAAACTCTGTGTCCTTCAGAAAAAGCTTTCTTTTGTGTCTCAAACATGTTCCCTAAACCATATATCCCTTTTCTTTTCCTTTTTCTACTTTCTTCTAGTTATTATCTTCCTTTAATCGTTCCCTTTTAAATGTCTTCTGGTTTGAGTATAATTTTCGTGTTGTTTTCTACCAGGAATCTTAAACGATATGTTCATCAAATGTAAAATATTCATCAAACATATGATTTAAAAGTCTATCGAAGAAGACAAAAGCGGGATTTTGTGTTGAAAGTGTTGTGTTACTAAAATAGAATTGAAATCTGATTTTATTTTCCATTTTTGAGTAAAAGAAATAATGGGGGAATTTGAAAGTAAACATTTTAGGGTCATAGTGGGAAGACTGTTTGAAAATAATGTGGTTATGTTGATTAAAAAAGGAGTAACATTAATGTATGAATTGAAAAGACATAAAAGTTAGTGCTGTCTGCCGAGTCATTTTGTCCTGAAAAAGTGGGATCAAAACTACTTTATTTCTCATACTAATCCAAAGAAAATATATATGTTTTTCAGGTCTTTGTTGCCGCTGTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAACCCTCAAATGCTTCATGGAAGCCAAACCAGTAATCATAAAGTTGCCTTATCCTCTCATTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGCAAGTCCTCTTTCTTTTCTATTTTCAACTTGATTTGGACGCCTCAAAGGACTTCACAACTTAACCTAAAGTTTCCTTAAACAAGCTTCAAATCTTTTGAACATAAAAGACAATTTCTCTATCAAAATTTGAAGTTTTGTACCAAAATTTAACTTCAGGTTCTTGGACAAGGCTTGTATCAAAAGCCTAGATGGAACAACCAGGGAAAGCCCATGGAGATTGTGCACTCCCACTCAAGTTGAGCAAGTGAAGATATTGATATCAGTTGTCCCAATATTTGCTTGCACGATTATATTCAACACAATATTGGCTCAGCTCCAAACATTTTCTGTTCAACAAGGGAGTTTGATGAATACTCAGCTCACCAAATCCTTCCACATTCCTCCCGCTTCCCTTCAGTCCATTCCTTACATTATGCTCATTTTCATTGTCCCTTTGTATGATACTTTCTTTGTCCCATTTGCTAGAAAGTTCACTGGCCACACCTCTGGAATCTCACCCTTAAAACGTATTGGCTTTGGTCTCTTCCTCTCCACATTTTCGATGGTTTCAGCAGCTATAATGGAGAAAAAGAGGAGAGATTCAGCTGTGAATTCGAACCAGACGATATCAATCTTCTGGATCACACCACAGTTCCTTATTTTTGGATTGTCAGAAATGTTCACAGCAGTTGGACTCATTGAGTTCTTTTACAAGCAATCAGTAAAAGGGATGCAAGCATTTTTAACAGCCATAACTTATTGCTCATACTCATTTGGGTTTTACTTGAGCTCATTGCTAGTGTCCATGGTGAATAAGATCACATCAAGCTCCACCAACGGAGGTTGGCTCCATGACAACAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTCAACTTTCTTAACTATCTCTTCTGGTCCAGTTGGTTCCAAGATCCCCCACCTCTCTCCCAAAAGCTACAACAAGATAATCTTCACCATGGAGAAAACCCACATTTACAACAACAATTTTTCAAAGAACAATGTTGA

mRNA sequence

TACCAAATGGAAATGGAAATGGAAATGGACATTGAGAGAAGGAGAGACACCCATCTCATGCCTTCTGAGGATCTCACTGTTGATTGGAGAGGAAGACCCTCCAACCCCGTCGCCCACGGCGGGATGAGAGCCGCTGCTTTTGTTCTCGGGCTGCAATCATTCGAGATAATGGCGATTGCTGCAGTTGGGAACAATCTGATAACATATGTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACGTAGTGACAAACTTTGTTGGGACTGTGTTTCTTTTGGCACTTCTTGGCGGTTTCCTTTCTGATTCCTATCTTGGGAGTTTTTGGACAATGCTTATCTTTGGCTTTGTTGAACTTTCTGGGTTCATATTACTATCAGTTCAGGCTCATCTTCCCCAACTAAAGCCACCCCAATGCAACATGCTAAGTGAAGGAGATGACTGCGTGGAAGCCAAGGGGATAAAGGCATTGATATTCTTTGTGGCACTCTACTTGGTGGCTCTTGGGAGTGGGTGTGTGAAGCCCAACATGATAGCTCATGGGGCTGATCAGTTCACTCCACAAGATCCAAGCCAATCCAAGAGGCTTTCAACATACTTCAATGCTGCTTATTTTGCCTTCTCAATTGGAGAGCTCATTGCCCTCACTCTTGTTGTTTGGATCCAAACCCATTCTGGGATGGACGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCTATGGGGTTGATCAGCTTGGTTGGCGGTATTTTTTACTACAGGAATAAACCCCCTCAAGGAGCCCTTTTTATCCCAATTCTTCAAGTCTTTGTTGCCGCTGTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAACCCTCAAATGCTTCATGGAAGCCAAACCAGTAATCATAAAGTTGCCTTATCCTCTCATTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGCAAGCTTGTATCAAAAGCCTAGATGGAACAACCAGGGAAAGCCCATGGAGATTGTGCACTCCCACTCAAGTTGAGCAAGTGAAGATATTGATATCAGTTGTCCCAATATTTGCTTGCACGATTATATTCAACACAATATTGGCTCAGCTCCAAACATTTTCTGTTCAACAAGGGAGTTTGATGAATACTCAGCTCACCAAATCCTTCCACATTCCTCCCGCTTCCCTTCAGTCCATTCCTTACATTATGCTCATTTTCATTGTCCCTTTGTATGATACTTTCTTTGTCCCATTTGCTAGAAAGTTCACTGGCCACACCTCTGGAATCTCACCCTTAAAACGTATTGGCTTTGGTCTCTTCCTCTCCACATTTTCGATGGTTTCAGCAGCTATAATGGAGAAAAAGAGGAGAGATTCAGCTGTGAATTCGAACCAGACGATATCAATCTTCTGGATCACACCACAGTTCCTTATTTTTGGATTGTCAGAAATGTTCACAGCAGTTGGACTCATTGAGTTCTTTTACAAGCAATCAGTAAAAGGGATGCAAGCATTTTTAACAGCCATAACTTATTGCTCATACTCATTTGGGTTTTACTTGAGCTCATTGCTAGTGTCCATGGTGAATAAGATCACATCAAGCTCCACCAACGGAGGTTGGCTCCATGACAACAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTCAACTTTCTTAACTATCTCTTCTGGTCCAGTTGGTTCCAAGATCCCCCACCTCTCTCCCAAAAGCTACAACAAGATAATCTTCACCATGGAGAAAACCCACATTTACAACAACAATTTTTCAAAGAACAATGTTGA

Coding sequence (CDS)

TACCAAATGGAAATGGAAATGGAAATGGACATTGAGAGAAGGAGAGACACCCATCTCATGCCTTCTGAGGATCTCACTGTTGATTGGAGAGGAAGACCCTCCAACCCCGTCGCCCACGGCGGGATGAGAGCCGCTGCTTTTGTTCTCGGGCTGCAATCATTCGAGATAATGGCGATTGCTGCAGTTGGGAACAATCTGATAACATATGTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACGTAGTGACAAACTTTGTTGGGACTGTGTTTCTTTTGGCACTTCTTGGCGGTTTCCTTTCTGATTCCTATCTTGGGAGTTTTTGGACAATGCTTATCTTTGGCTTTGTTGAACTTTCTGGGTTCATATTACTATCAGTTCAGGCTCATCTTCCCCAACTAAAGCCACCCCAATGCAACATGCTAAGTGAAGGAGATGACTGCGTGGAAGCCAAGGGGATAAAGGCATTGATATTCTTTGTGGCACTCTACTTGGTGGCTCTTGGGAGTGGGTGTGTGAAGCCCAACATGATAGCTCATGGGGCTGATCAGTTCACTCCACAAGATCCAAGCCAATCCAAGAGGCTTTCAACATACTTCAATGCTGCTTATTTTGCCTTCTCAATTGGAGAGCTCATTGCCCTCACTCTTGTTGTTTGGATCCAAACCCATTCTGGGATGGACGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCTATGGGGTTGATCAGCTTGGTTGGCGGTATTTTTTACTACAGGAATAAACCCCCTCAAGGAGCCCTTTTTATCCCAATTCTTCAAGTCTTTGTTGCCGCTGTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAACCCTCAAATGCTTCATGGAAGCCAAACCAGTAATCATAAAGTTGCCTTATCCTCTCATTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGCAAGCTTGTATCAAAAGCCTAGATGGAACAACCAGGGAAAGCCCATGGAGATTGTGCACTCCCACTCAAGTTGAGCAAGTGAAGATATTGATATCAGTTGTCCCAATATTTGCTTGCACGATTATATTCAACACAATATTGGCTCAGCTCCAAACATTTTCTGTTCAACAAGGGAGTTTGATGAATACTCAGCTCACCAAATCCTTCCACATTCCTCCCGCTTCCCTTCAGTCCATTCCTTACATTATGCTCATTTTCATTGTCCCTTTGTATGATACTTTCTTTGTCCCATTTGCTAGAAAGTTCACTGGCCACACCTCTGGAATCTCACCCTTAAAACGTATTGGCTTTGGTCTCTTCCTCTCCACATTTTCGATGGTTTCAGCAGCTATAATGGAGAAAAAGAGGAGAGATTCAGCTGTGAATTCGAACCAGACGATATCAATCTTCTGGATCACACCACAGTTCCTTATTTTTGGATTGTCAGAAATGTTCACAGCAGTTGGACTCATTGAGTTCTTTTACAAGCAATCAGTAAAAGGGATGCAAGCATTTTTAACAGCCATAACTTATTGCTCATACTCATTTGGGTTTTACTTGAGCTCATTGCTAGTGTCCATGGTGAATAAGATCACATCAAGCTCCACCAACGGAGGTTGGCTCCATGACAACAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTCAACTTTCTTAACTATCTCTTCTGGTCCAGTTGGTTCCAAGATCCCCCACCTCTCTCCCAAAAGCTACAACAAGATAATCTTCACCATGGAGAAAACCCACATTTACAACAACAATTTTTCAAAGAACAATGTTGA

Protein sequence

YQMEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRQACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFFKEQC
Homology
BLAST of CmaCh04G008850 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.2e-234
Identity = 416/554 (75.09%), Postives = 477/554 (86.10%), Query Frame = 0

Query: 22  SEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKS 81
           +E+ +VDWRGRPSNP  HGGMRAA FVLGLQ+FEIM IAAVGNNLITYVINEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 82  ANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQC 141
           AN+VTNFVGT+F+ ALLGG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+C
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKC 144

Query: 142 NMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTY 201
           N L +   C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF+   P QSKRLS+Y
Sbjct: 145 NPLID-QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSY 204

Query: 202 FNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQG 261
           FNAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV G  Y+RNK P+ 
Sbjct: 205 FNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRR 264

Query: 262 ALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIP-TQKFR---QA 321
           ++F PI  V VAA+LKRK   PS+P+MLHG    +H VA     ++ +P T +FR   +A
Sbjct: 265 SIFTPIAHVIVAAILKRKLASPSDPRMLHG----DHHVANDVVPSSTLPHTPRFRFLDKA 324

Query: 322 CIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 381
           CIK  D  T+ESPWRLCT TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS MN
Sbjct: 325 CIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 384

Query: 382 TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 441
           T+L+ SFHIPPASLQ+IPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLS
Sbjct: 385 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 444

Query: 442 TFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 501
           TFSMVSAA++EKKRRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS KGM
Sbjct: 445 TFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGM 504

Query: 502 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG-GWLHDNNLNKDRLDLFYWLLAAL 561
           ++FL A+TYCSYSFGFY SS+LVS+VNKITS+S +  GWL +N+LNKDRLDLFYWLLA L
Sbjct: 505 ESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVL 564

Query: 562 SFLNFLNYLFWSSW 571
           S LNFL+YLFWS W
Sbjct: 565 SLLNFLSYLFWSRW 573

BLAST of CmaCh04G008850 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.4e-222
Identity = 402/580 (69.31%), Postives = 473/580 (81.55%), Query Frame = 0

Query: 23  EDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSA 82
           +DL VD+RGRP  P  HGG RAA FVLG Q+FE+MAIAAVGNNLITYV NEMHF LSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 83  NVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 142
           N+VTNF+GTVFLL+LLGGFLSDSYLGSF TML+FG +E+SGFILLSVQAHLP+L+PP+CN
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 143 MLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYF 202
           M S    CVEA G KA   + AL LVALGSGC+KPN+I+HGA+QF  +D    ++LS++F
Sbjct: 143 MKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFF 202

Query: 203 NAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGA 262
           NAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+ISLV G  +YRNKPP G+
Sbjct: 203 NAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS 262

Query: 263 LFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFR---QACI 322
           +F PI QVFVAA+ KRKQ+CPSNP M+H   T   +V    HS       KFR   +ACI
Sbjct: 263 IFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHS------NKFRFLDKACI 322

Query: 323 KSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQ 382
           K+  G   ESPWRLCT  QV QVKIL+SV+PIFACTIIFNTILAQLQTFSVQQGS MNT 
Sbjct: 323 KT-QGKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTH 382

Query: 383 LTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTF 442
           +TK+F IPPASLQ+IPYI+LIF VPLY+TFFVP ARK TG+ SGISPL+RIG GLFL+TF
Sbjct: 383 ITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATF 442

Query: 443 SMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQA 502
           SMV+AA++EKKRR+S +  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFYKQS + MQ+
Sbjct: 443 SMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQS 502

Query: 503 FLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG---GWLHDNNLNKDRLDLFYWLLAAL 562
           FLTA+TYCSYSFGFYLSS+LVS VN++TSS+ +G   GWL DN+LNKDRLD FYWLLA+L
Sbjct: 503 FLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASL 562

Query: 563 SFLNFLNYLFWSSWFQ-DPPPLSQKLQQDNLHHGENPHLQ 596
           SF+NF NYLFWS W+  DP       + ++L   EN  ++
Sbjct: 563 SFINFFNYLFWSRWYSCDPSATHHSAEVNSLEALENGEIK 592

BLAST of CmaCh04G008850 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.8e-113
Identity = 237/564 (42.02%), Postives = 340/564 (60.28%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+GT FLLALLGGFLSD++  +F   LI   +E  G I+L++QA  P L PP C    + 
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC----DS 134

Query: 148 DDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C E  G KA + FV LYLVALG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVF 194

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGALFIPI 267
             + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G+    I
Sbjct: 195 CLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTI 254

Query: 268 LQVFVAAVLK------------RKQVCPSNPQMLHGSQTSNHKVALSS-HSANLIPTQKF 327
           L+V +AA +K               V PSN  +  G +    +  L        +P +  
Sbjct: 255 LKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQ 314

Query: 328 RQACIKSLDGTTRESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQ 387
               +K L+G   E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL TFSVQ
Sbjct: 315 LTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQ 374

Query: 388 QGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIG 447
           Q + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L+RIG
Sbjct: 375 QAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIG 434

Query: 448 FGLFLSTFSMVSAAIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVG 507
            GL LS  +M  AA++E KR+  A     ++S +T  ++  WI  Q+L  G +++FT  G
Sbjct: 435 VGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAG 494

Query: 508 LIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKD 567
           L+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N+ 
Sbjct: 495 LLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSINRY 554

Query: 568 RLDLFYWLLAALSFLNFLNYLFWS 569
           +LD FYWL+  LS  NFL+YLFW+
Sbjct: 555 KLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of CmaCh04G008850 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 6.2e-106
Identity = 230/582 (39.52%), Postives = 346/582 (59.45%), Query Frame = 0

Query: 3   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           ME+EM  D+ +              DWR + +    HGGM AA+FVL ++  E +A  A 
Sbjct: 1   MEVEMHGDVSKWEG---------YADWRNKAALRGRHGGMLAASFVLAVEILENLAFLAN 60

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
            +NL+ Y+ N MH SL++S++ VT F+ T FLLALLGGFL+D++  +F   LI   +E  
Sbjct: 61  ASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFL 120

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           G ILL++QA  P L PP C   S    C    G KA   FV LYLV+LG G +K ++ +H
Sbjct: 121 GLILLTIQARRPSLMPPPCKS-SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSH 180

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GA+QF    P   K+ ST+FN   F  S G L+A+T VVWI+ + G + GFGVS   + +
Sbjct: 181 GAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFL 240

Query: 243 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA-- 302
            ++  + G  +Y+NK P+G+    I +V +AA +     C S         +SNH  +  
Sbjct: 241 SILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVS---CSS-------KTSSNHFTSRE 300

Query: 303 LSSHSANLIPTQKFRQA--CI-KSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTII 362
           + S      P+Q    +  C+ K+++G T    W  CT  QVE VKI++ ++PIF CTI+
Sbjct: 301 VQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIM 360

Query: 363 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 422
            N  LAQL T+SV Q + MN ++  +F++P ASL   P + ++ + P YD   +PFARK 
Sbjct: 361 LNCCLAQLSTYSVHQAATMNRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKV 420

Query: 423 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA-----VNSNQT--ISIFWITPQ 482
           T    GI+ L+RIG GL LS  +M  AA++E KR+  A     ++S +T  I+  WI  Q
Sbjct: 421 TKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQ 480

Query: 483 FLIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSS 542
           +L  G +++FT  GL+EFF+ ++   M++  T++++ S + G+YLSS++V +VN++T S+
Sbjct: 481 YLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSA 540

Query: 543 TNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQ 573
               WL +  LN++RLDLFYWL+  LS +NFL+YLFW+  ++
Sbjct: 541 GQSPWLGE-KLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYK 559

BLAST of CmaCh04G008850 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.7e-101
Identity = 208/560 (37.14%), Postives = 333/560 (59.46%), Query Frame = 0

Query: 22  SEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKS 81
           ++D TVD    P+N    G  +A  F+LG +  E +A   +G NL+ Y+ + ++   + +
Sbjct: 8   TQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATA 67

Query: 82  ANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQC 141
           AN VTN+ GT ++  L+G F++D+YLG +WT+  F F+ +SG  LL++ A +P LKP  C
Sbjct: 68  ANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC 127

Query: 142 NMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTY 201
           N      D       +  +FFVALY++ALG+G +KP + + GADQF   D ++  + S++
Sbjct: 128 NA-----DTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSF 187

Query: 202 FNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQG 261
           FN  YF+ ++G LIA T++VWIQ + G   GFGV    M + +     G  +YR + P G
Sbjct: 188 FNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGG 247

Query: 262 ALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRQACIKS 321
           +    I QV VAA  K     P +  +L  +      +  S    +    + F +A ++S
Sbjct: 248 SPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVES 307

Query: 322 LDGTTRE---SPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNT 381
              + ++   +PWRLC+ TQVE++K +I+++P++A  I+F T+ +Q+ T  V QG+ M+ 
Sbjct: 308 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 367

Query: 382 QLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLST 441
            + K+F IP ASL     + ++F  P+YD F +P ARKFT +  G + L+R+G GL +S 
Sbjct: 368 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 427

Query: 442 FSMVSAAIMEKKRRDSAVNSNQ------TISIFWITPQFLIFGLSEMFTAVGLIEFFYKQ 501
           F+M++A ++E  R D     N        +SIFW  PQ+L+ G +E+FT +G +EFFY Q
Sbjct: 428 FAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQ 487

Query: 502 SVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWL 561
           +   M++  +A++  + + G YLS++LV++V KIT  +   GW+ D NLN+  LD F++L
Sbjct: 488 APDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYFFYL 547

Query: 562 LAALSFLNFLNYLFWSSWFQ 573
           LA LSFLNFL YL+ S  ++
Sbjct: 548 LATLSFLNFLVYLWISKRYK 561

BLAST of CmaCh04G008850 vs. ExPASy TrEMBL
Match: A0A6J1JRW4 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE=3 SV=1)

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 600/604 (99.34%), Postives = 601/604 (99.50%), Query Frame = 0

Query: 3   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV
Sbjct: 1   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 60

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
           GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS
Sbjct: 61  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 120

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH
Sbjct: 121 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 180

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM
Sbjct: 181 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 240

Query: 243 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 302
           GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS
Sbjct: 241 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 300

Query: 303 SHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 362
           SHSANLIPTQKFR   +ACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SHSANLIPTQKFRFLDKACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 363 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 422
           TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 423 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 482
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 480

Query: 483 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 542
           FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 540

Query: 543 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF 602
           NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF
Sbjct: 541 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF 600

Query: 603 KEQC 604
           KEQC
Sbjct: 601 KEQC 604

BLAST of CmaCh04G008850 vs. ExPASy TrEMBL
Match: A0A6J1FR74 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 PE=3 SV=1)

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 591/605 (97.69%), Postives = 597/605 (98.68%), Query Frame = 0

Query: 2   QMEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA 61
           +MEM+M+MDIERRRDTHLMPS+DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA
Sbjct: 2   EMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA 61

Query: 62  VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL 121
           VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL
Sbjct: 62  VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL 121

Query: 122 SGFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIA 181
           SGFILLSVQAHLPQLKPPQCNMLSEGD CVEAKGIKALIFFVALYLVALGSGCVKPNMIA
Sbjct: 122 SGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIA 181

Query: 182 HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA 241
           HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA
Sbjct: 182 HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA 241

Query: 242 MGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL 301
           MGLISLVGGIFYYRNKPPQGA+FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL
Sbjct: 242 MGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL 301

Query: 302 SSHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIF 361
           SSHSANLIPTQKFR   +ACIKS DGTTRESPWRLCT TQVEQVKILISVVPIFACTIIF
Sbjct: 302 SSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIF 361

Query: 362 NTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFT 421
           NTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFT
Sbjct: 362 NTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFT 421

Query: 422 GHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSE 481
           GHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSE
Sbjct: 422 GHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSE 481

Query: 482 MFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHD 541
           MFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHD
Sbjct: 482 MFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHD 541

Query: 542 NNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQF 601
           NNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPP LSQKLQQDNLHHGEN HLQQQF
Sbjct: 542 NNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQF 601

Query: 602 FKEQC 604
           FKEQC
Sbjct: 602 FKEQC 606

BLAST of CmaCh04G008850 vs. ExPASy TrEMBL
Match: A0A6J1EMI3 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435878 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 9.7e-296
Identity = 525/601 (87.35%), Postives = 557/601 (92.68%), Query Frame = 0

Query: 9   MDIERRRD----------THLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMA 68
           M++ERRRD           + MPSED+TVDWRGRPSN V+HGGMRAAAFVLGLQ+FEIMA
Sbjct: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60

Query: 69  IAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGF 128
           IAAVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLAL+GGFLSDSYLGSFWTMLIFGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 129 VELSGFILLSVQAHLPQLKPPQCNML-SEGDDCVEAKGIKALIFFVALYLVALGSGCVKP 188
           VELSGFILLSVQAH+PQLKPPQCNML S+G++CVEAKG+KALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 189 NMIAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSA 248
           NMIAHGADQF+PQ P+QSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 249 AVMAMGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNH 308
           AVMA+GLISLV GIFYYRNKPPQG +F PILQVFVAA+LKRKQVCPSNP MLHGSQTSN 
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 309 KVALSSHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFAC 368
           KV LSSHSANLIPTQKFR   +ACIK+ D   +ESPWRLCTP QVEQVKIL+SVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 369 TIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFA 428
           TI+FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLI IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 429 RKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIF 488
           RKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSAVN NQTISIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 489 GLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGG 548
           GLSEMFTAVGLIEFFY QS+KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+NGG
Sbjct: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 549 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQ-QDNLHHGENPH 595
           WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWS WF + P +SQK Q  DN HHGE+ H
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

BLAST of CmaCh04G008850 vs. ExPASy TrEMBL
Match: A0A6J1JCZ7 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483371 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.4e-294
Identity = 524/599 (87.48%), Postives = 554/599 (92.49%), Query Frame = 0

Query: 3   MEMEMEMDI--ERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 62
           MEME   D   E   + + MPSED+TVDWRGRPSN V+HGGMRAAAFVLGLQ+FEIMAIA
Sbjct: 1   MEMERRRDFKGENNANANAMPSEDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMAIA 60

Query: 63  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 122
           AVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLAL+GGFLSDSYLGSFWTMLIFGFVE
Sbjct: 61  AVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVE 120

Query: 123 LSGFILLSVQAHLPQLKPPQCNML-SEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNM 182
           LSGFILLSVQAH+PQLKPPQCNML S+G++CVEAKG+KALIFFVALYLVALGSGCVKPNM
Sbjct: 121 LSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKPNM 180

Query: 183 IAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAV 242
           IAHGADQF+PQ PSQSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVSAAV
Sbjct: 181 IAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAV 240

Query: 243 MAMGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKV 302
           MA+GLISLV GIFYYRNKPPQG +F PILQVFVAA+LKRKQVCPSNP MLHGSQTSN KV
Sbjct: 241 MALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKV 300

Query: 303 ALSSHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTI 362
            LSSHSANLIPTQKFR   +ACIK+ D   +ESPWRLCTP QVEQVKIL+SVVPIFACTI
Sbjct: 301 GLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTI 360

Query: 363 IFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARK 422
           +FNTILAQLQTFSVQQGSLMNTQLTKS HIPPASLQSIPYIMLI IVPLYDTFFVPFARK
Sbjct: 361 VFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFARK 420

Query: 423 FTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGL 482
            TGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSAVN NQTISIFWITPQFLIFGL
Sbjct: 421 ITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGL 480

Query: 483 SEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWL 542
           SEMFTAVGLIEFFYKQS+KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+NGGWL
Sbjct: 481 SEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWL 540

Query: 543 HDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQ-QDNLHHGENPHL 595
           HDNNLNKDRLDLFYWLLAALSFLN LNYLFWS WF + P +SQK Q  DN HHGE+ H+
Sbjct: 541 HDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHI 599

BLAST of CmaCh04G008850 vs. ExPASy TrEMBL
Match: A0A6J1C7K1 (protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146 PE=3 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.5e-293
Identity = 526/595 (88.40%), Postives = 558/595 (93.78%), Query Frame = 0

Query: 7   MEMDIERRRDTHLMPSED-LTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNN 66
           MEM++ERR+D       D +TVDWRGRPSNP++HGGMRAAAFVLGLQ+FEIMAIAAVGNN
Sbjct: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60

Query: 67  LITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFI 126
           LITY INEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTML+FGFVELSGFI
Sbjct: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120

Query: 127 LLSVQAHLPQLKPPQCNMLSE-GDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGA 186
           LLSVQAHLPQLKPPQCNMLSE G++C EAKG+KALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180

Query: 187 DQFTPQ-DPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 246
           DQF+PQ +P  SK+LSTYFNAAYFAFS+GELIALTLVVWIQTHSGMDVGFGVSAAVMAMG
Sbjct: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240

Query: 247 LISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS-NHKVALS 306
           LISLVGGIFYYRNKPPQG +F PILQVFVAA+LKRKQVCPSNP MLHGSQ + +HKVALS
Sbjct: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300

Query: 307 SHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 366
           S SANLIPTQKFR   +ACIKS DG T+ESPWRLCTPTQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SDSANLIPTQKFRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 367 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 426
           TILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 427 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 486
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA+N NQT+SIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480

Query: 487 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 546
           FTAVGLIEFFYKQS KGMQAFLTAITYCSYSFGFYLSSLLVSMVNKIT++S+NGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540

Query: 547 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQD-NLHHGENPH 594
           NLNKDRLDLFYWLLA LSFLNFLNYLFWS+WF + P LSQK   D N HHGE+ H
Sbjct: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 595

BLAST of CmaCh04G008850 vs. NCBI nr
Match: XP_022993122.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita maxima])

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 600/604 (99.34%), Postives = 601/604 (99.50%), Query Frame = 0

Query: 3   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV
Sbjct: 1   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 60

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
           GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS
Sbjct: 61  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 120

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH
Sbjct: 121 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 180

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM
Sbjct: 181 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 240

Query: 243 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 302
           GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS
Sbjct: 241 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 300

Query: 303 SHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 362
           SHSANLIPTQKFR   +ACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SHSANLIPTQKFRFLDKACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 363 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 422
           TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 423 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 482
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 480

Query: 483 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 542
           FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 540

Query: 543 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF 602
           NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF
Sbjct: 541 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF 600

Query: 603 KEQC 604
           KEQC
Sbjct: 601 KEQC 604

BLAST of CmaCh04G008850 vs. NCBI nr
Match: XP_022942404.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita moschata])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 591/605 (97.69%), Postives = 597/605 (98.68%), Query Frame = 0

Query: 2   QMEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA 61
           +MEM+M+MDIERRRDTHLMPS+DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA
Sbjct: 2   EMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA 61

Query: 62  VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL 121
           VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL
Sbjct: 62  VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL 121

Query: 122 SGFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIA 181
           SGFILLSVQAHLPQLKPPQCNMLSEGD CVEAKGIKALIFFVALYLVALGSGCVKPNMIA
Sbjct: 122 SGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIA 181

Query: 182 HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA 241
           HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA
Sbjct: 182 HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA 241

Query: 242 MGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL 301
           MGLISLVGGIFYYRNKPPQGA+FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL
Sbjct: 242 MGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL 301

Query: 302 SSHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIF 361
           SSHSANLIPTQKFR   +ACIKS DGTTRESPWRLCT TQVEQVKILISVVPIFACTIIF
Sbjct: 302 SSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIF 361

Query: 362 NTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFT 421
           NTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFT
Sbjct: 362 NTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFT 421

Query: 422 GHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSE 481
           GHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSE
Sbjct: 422 GHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSE 481

Query: 482 MFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHD 541
           MFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHD
Sbjct: 482 MFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHD 541

Query: 542 NNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQF 601
           NNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPP LSQKLQQDNLHHGEN HLQQQF
Sbjct: 542 NNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQF 601

Query: 602 FKEQC 604
           FKEQC
Sbjct: 602 FKEQC 606

BLAST of CmaCh04G008850 vs. NCBI nr
Match: KAG6600707.1 (Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031346.1 Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 590/603 (97.84%), Postives = 596/603 (98.84%), Query Frame = 0

Query: 2   QMEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA 61
           +MEMEM+MDIERRRDTHLMPS+DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA
Sbjct: 4   EMEMEMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAA 63

Query: 62  VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL 121
           VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL
Sbjct: 64  VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVEL 123

Query: 122 SGFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIA 181
           SGFILLSVQAHLPQLKPPQCNM+SEGD CVEAKGIKALIFFVALYLVALGSGCVKPNMIA
Sbjct: 124 SGFILLSVQAHLPQLKPPQCNMVSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIA 183

Query: 182 HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA 241
           HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA
Sbjct: 184 HGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMA 243

Query: 242 MGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL 301
           MGLISLVGGIFYYRNKPPQGA+FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL
Sbjct: 244 MGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVAL 303

Query: 302 SSHSANLIPTQKF-RQACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNT 361
           SSHSANLIPTQKF  +ACIKS DGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNT
Sbjct: 304 SSHSANLIPTQKFLDKACIKSQDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNT 363

Query: 362 ILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGH 421
           ILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGH
Sbjct: 364 ILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGH 423

Query: 422 TSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMF 481
           TSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMF
Sbjct: 424 TSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMF 483

Query: 482 TAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNN 541
           TAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNN
Sbjct: 484 TAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNN 543

Query: 542 LNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFFK 601
           LNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPP LSQKLQQDNLHH EN HLQQQFFK
Sbjct: 544 LNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHEENSHLQQQFFK 603

Query: 602 EQC 604
           EQC
Sbjct: 604 EQC 606

BLAST of CmaCh04G008850 vs. NCBI nr
Match: XP_023530877.1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 580/602 (96.35%), Postives = 589/602 (97.84%), Query Frame = 0

Query: 3   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           MEM+M+MDIERRRDTHLMPS+DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV
Sbjct: 5   MEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 64

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
           GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS
Sbjct: 65  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 124

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           GFILLSVQAHLPQLKPPQCNMLSEGD CVEAKGIKALIFFVALYLVALGSGCVKPNMIAH
Sbjct: 125 GFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 184

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GADQFTPQ PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM
Sbjct: 185 GADQFTPQHPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 244

Query: 243 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 302
           GLISLVGGIFYYRNKPPQGA+FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS
Sbjct: 245 GLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 304

Query: 303 SHSANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 362
           SHSANLIPTQKFR   +AC+KS DGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN
Sbjct: 305 SHSANLIPTQKFRFLDKACVKSQDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 364

Query: 363 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 422
           TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 365 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 424

Query: 423 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 482
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAV+SNQTISIFWITPQFLIFGLSEM
Sbjct: 425 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVDSNQTISIFWITPQFLIFGLSEM 484

Query: 483 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 542
           FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN
Sbjct: 485 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 544

Query: 543 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF 602
           NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPP LSQKLQQDNLHHGEN H+    F
Sbjct: 545 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENXHIYNNNF 604

BLAST of CmaCh04G008850 vs. NCBI nr
Match: XP_038903187.1 (protein NRT1/ PTR FAMILY 4.3-like [Benincasa hispida])

HSP 1 Score: 1030.0 bits (2662), Expect = 8.1e-297
Identity = 526/591 (89.00%), Postives = 557/591 (94.25%), Query Frame = 0

Query: 5   MEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGN 64
           MEME   + + ++H +PSED++VDWRGRPSNP +HGGMRAAAFVLGLQ+FEIMAIAAVGN
Sbjct: 1   MEMERKRDFKGESHTIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGN 60

Query: 65  NLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGF 124
           NLITY+INEMHFSLSKSAN+VTNFVGTVFLLALLGGFLSDSYLGSFWT+LIFGFVELSGF
Sbjct: 61  NLITYLINEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVELSGF 120

Query: 125 ILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGA 184
           ILLSVQAH+PQLKPPQCNML + D+CVEAKG+KALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 121 ILLSVQAHVPQLKPPQCNMLLKSDECVEAKGVKALIFFVALYLVALGSGCVKPNMIAHGA 180

Query: 185 DQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGL 244
           DQF+PQ+ SQSK LS YFNAAYFAFSIGELIALTL+VWIQTHSGMDVGFGVSAAVMA+GL
Sbjct: 181 DQFSPQNSSQSKSLSKYFNAAYFAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGL 240

Query: 245 ISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSH 304
           I LV GIFYYRNKPPQG +FIPILQVFVAA+LKRKQVCPSN  MLHGSQTSN KVALSS 
Sbjct: 241 IILVAGIFYYRNKPPQGPIFIPILQVFVAALLKRKQVCPSNSHMLHGSQTSNDKVALSSD 300

Query: 305 SANLIPTQKFR---QACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTI 364
           SANLIPTQKFR   +AC KS DG T+ESPWRLCTP QVEQVKILISVVPIFACTIIFNTI
Sbjct: 301 SANLIPTQKFRFLDKACFKSQDGATKESPWRLCTPNQVEQVKILISVVPIFACTIIFNTI 360

Query: 365 LAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHT 424
           LAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHT
Sbjct: 361 LAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHT 420

Query: 425 SGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFT 484
           SGISPLKRIGFGLFLSTFSMVSAAIMEK+RRDSAVN NQT+SIFWITPQFLIFGLSEMFT
Sbjct: 421 SGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTMSIFWITPQFLIFGLSEMFT 480

Query: 485 AVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNL 544
           AVGLIEFFYKQSVKGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+NGGWLHDNNL
Sbjct: 481 AVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNL 540

Query: 545 NKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQ-QDNLHHGEN 592
           NKDRLDLFYWLLAALSFLNFLNYLFWS+WF + P LSQKLQ  DN H GE+
Sbjct: 541 NKDRLDLFYWLLAALSFLNFLNYLFWSTWFDEHPSLSQKLQNDDNPHQGED 591

BLAST of CmaCh04G008850 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 813.9 bits (2101), Expect = 8.5e-236
Identity = 416/554 (75.09%), Postives = 477/554 (86.10%), Query Frame = 0

Query: 22  SEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKS 81
           +E+ +VDWRGRPSNP  HGGMRAA FVLGLQ+FEIM IAAVGNNLITYVINEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 82  ANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQC 141
           AN+VTNFVGT+F+ ALLGG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+C
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKC 144

Query: 142 NMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTY 201
           N L +   C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF+   P QSKRLS+Y
Sbjct: 145 NPLID-QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSY 204

Query: 202 FNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQG 261
           FNAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV G  Y+RNK P+ 
Sbjct: 205 FNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRR 264

Query: 262 ALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIP-TQKFR---QA 321
           ++F PI  V VAA+LKRK   PS+P+MLHG    +H VA     ++ +P T +FR   +A
Sbjct: 265 SIFTPIAHVIVAAILKRKLASPSDPRMLHG----DHHVANDVVPSSTLPHTPRFRFLDKA 324

Query: 322 CIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 381
           CIK  D  T+ESPWRLCT TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS MN
Sbjct: 325 CIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 384

Query: 382 TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 441
           T+L+ SFHIPPASLQ+IPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLS
Sbjct: 385 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 444

Query: 442 TFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 501
           TFSMVSAA++EKKRRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS KGM
Sbjct: 445 TFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGM 504

Query: 502 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG-GWLHDNNLNKDRLDLFYWLLAAL 561
           ++FL A+TYCSYSFGFY SS+LVS+VNKITS+S +  GWL +N+LNKDRLDLFYWLLA L
Sbjct: 505 ESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVL 564

Query: 562 SFLNFLNYLFWSSW 571
           S LNFL+YLFWS W
Sbjct: 565 SLLNFLSYLFWSRW 573

BLAST of CmaCh04G008850 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 773.9 bits (1997), Expect = 9.7e-224
Identity = 402/580 (69.31%), Postives = 473/580 (81.55%), Query Frame = 0

Query: 23  EDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSA 82
           +DL VD+RGRP  P  HGG RAA FVLG Q+FE+MAIAAVGNNLITYV NEMHF LSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 83  NVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 142
           N+VTNF+GTVFLL+LLGGFLSDSYLGSF TML+FG +E+SGFILLSVQAHLP+L+PP+CN
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 143 MLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYF 202
           M S    CVEA G KA   + AL LVALGSGC+KPN+I+HGA+QF  +D    ++LS++F
Sbjct: 143 MKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFF 202

Query: 203 NAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGA 262
           NAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+ISLV G  +YRNKPP G+
Sbjct: 203 NAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS 262

Query: 263 LFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFR---QACI 322
           +F PI QVFVAA+ KRKQ+CPSNP M+H   T   +V    HS       KFR   +ACI
Sbjct: 263 IFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHS------NKFRFLDKACI 322

Query: 323 KSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQ 382
           K+  G   ESPWRLCT  QV QVKIL+SV+PIFACTIIFNTILAQLQTFSVQQGS MNT 
Sbjct: 323 KT-QGKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTH 382

Query: 383 LTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTF 442
           +TK+F IPPASLQ+IPYI+LIF VPLY+TFFVP ARK TG+ SGISPL+RIG GLFL+TF
Sbjct: 383 ITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATF 442

Query: 443 SMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQA 502
           SMV+AA++EKKRR+S +  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFYKQS + MQ+
Sbjct: 443 SMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQS 502

Query: 503 FLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG---GWLHDNNLNKDRLDLFYWLLAAL 562
           FLTA+TYCSYSFGFYLSS+LVS VN++TSS+ +G   GWL DN+LNKDRLD FYWLLA+L
Sbjct: 503 FLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASL 562

Query: 563 SFLNFLNYLFWSSWFQ-DPPPLSQKLQQDNLHHGENPHLQ 596
           SF+NF NYLFWS W+  DP       + ++L   EN  ++
Sbjct: 563 SFINFFNYLFWSRWYSCDPSATHHSAEVNSLEALENGEIK 592

BLAST of CmaCh04G008850 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 409.5 bits (1051), Expect = 4.8e-114
Identity = 237/564 (42.02%), Postives = 340/564 (60.28%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+GT FLLALLGGFLSD++  +F   LI   +E  G I+L++QA  P L PP C    + 
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC----DS 134

Query: 148 DDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C E  G KA + FV LYLVALG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVF 194

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGALFIPI 267
             + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G+    I
Sbjct: 195 CLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTI 254

Query: 268 LQVFVAAVLK------------RKQVCPSNPQMLHGSQTSNHKVALSS-HSANLIPTQKF 327
           L+V +AA +K               V PSN  +  G +    +  L        +P +  
Sbjct: 255 LKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQ 314

Query: 328 RQACIKSLDGTTRESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQ 387
               +K L+G   E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL TFSVQ
Sbjct: 315 LTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQ 374

Query: 388 QGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIG 447
           Q + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L+RIG
Sbjct: 375 QAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIG 434

Query: 448 FGLFLSTFSMVSAAIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVG 507
            GL LS  +M  AA++E KR+  A     ++S +T  ++  WI  Q+L  G +++FT  G
Sbjct: 435 VGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAG 494

Query: 508 LIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKD 567
           L+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N+ 
Sbjct: 495 LLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSINRY 554

Query: 568 RLDLFYWLLAALSFLNFLNYLFWS 569
           +LD FYWL+  LS  NFL+YLFW+
Sbjct: 555 KLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of CmaCh04G008850 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.5e-107
Identity = 225/557 (40.39%), Postives = 338/557 (60.68%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y+ N MH SL++S++ VT 
Sbjct: 13  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 72

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+ T FLLALLGGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS-SAA 132

Query: 148 DDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C    G KA   FV LYLV+LG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 133 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 192

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGALFIPI 267
             S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G+    I
Sbjct: 193 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 252

Query: 268 LQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA--LSSHSANLIPTQKFRQA--CI-KSL 327
            +V +AA +     C S         +SNH  +  + S      P+Q    +  C+ K++
Sbjct: 253 FKVLLAASIVS---CSS-------KTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI 312

Query: 328 DGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQLTK 387
           +G T    W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  
Sbjct: 313 EGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV- 372

Query: 388 SFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMV 447
           +F++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M 
Sbjct: 373 NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMA 432

Query: 448 SAAIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVK 507
            AA++E KR+  A     ++S +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++  
Sbjct: 433 VAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPS 492

Query: 508 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAA 567
            M++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  
Sbjct: 493 SMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCV 552

Query: 568 LSFLNFLNYLFWSSWFQ 573
           LS +NFL+YLFW+  ++
Sbjct: 553 LSVVNFLHYLFWAKRYK 555

BLAST of CmaCh04G008850 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 4.4e-107
Identity = 230/582 (39.52%), Postives = 346/582 (59.45%), Query Frame = 0

Query: 3   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           ME+EM  D+ +              DWR + +    HGGM AA+FVL ++  E +A  A 
Sbjct: 1   MEVEMHGDVSKWEG---------YADWRNKAALRGRHGGMLAASFVLAVEILENLAFLAN 60

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
            +NL+ Y+ N MH SL++S++ VT F+ T FLLALLGGFL+D++  +F   LI   +E  
Sbjct: 61  ASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFL 120

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           G ILL++QA  P L PP C   S    C    G KA   FV LYLV+LG G +K ++ +H
Sbjct: 121 GLILLTIQARRPSLMPPPCKS-SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSH 180

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GA+QF    P   K+ ST+FN   F  S G L+A+T VVWI+ + G + GFGVS   + +
Sbjct: 181 GAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFL 240

Query: 243 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA-- 302
            ++  + G  +Y+NK P+G+    I +V +AA +     C S         +SNH  +  
Sbjct: 241 SILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVS---CSS-------KTSSNHFTSRE 300

Query: 303 LSSHSANLIPTQKFRQA--CI-KSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTII 362
           + S      P+Q    +  C+ K+++G T    W  CT  QVE VKI++ ++PIF CTI+
Sbjct: 301 VQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIM 360

Query: 363 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 422
            N  LAQL T+SV Q + MN ++  +F++P ASL   P + ++ + P YD   +PFARK 
Sbjct: 361 LNCCLAQLSTYSVHQAATMNRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKV 420

Query: 423 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA-----VNSNQT--ISIFWITPQ 482
           T    GI+ L+RIG GL LS  +M  AA++E KR+  A     ++S +T  I+  WI  Q
Sbjct: 421 TKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQ 480

Query: 483 FLIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSS 542
           +L  G +++FT  GL+EFF+ ++   M++  T++++ S + G+YLSS++V +VN++T S+
Sbjct: 481 YLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSA 540

Query: 543 TNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQ 573
               WL +  LN++RLDLFYWL+  LS +NFL+YLFW+  ++
Sbjct: 541 GQSPWLGE-KLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYK 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VV51.2e-23475.09Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ61.4e-22269.31Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q8H1576.8e-11342.02Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE46.2e-10639.52Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9M3902.7e-10137.14Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JRW40.0e+0099.34protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE... [more]
A0A6J1FR740.0e+0097.69protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 ... [more]
A0A6J1EMI39.7e-29687.35protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JCZ72.4e-29487.48protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1C7K11.5e-29388.40protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146... [more]
Match NameE-valueIdentityDescription
XP_022993122.10.0e+0099.34protein NRT1/ PTR FAMILY 4.3-like [Cucurbita maxima][more]
XP_022942404.10.0e+0097.69protein NRT1/ PTR FAMILY 4.3-like [Cucurbita moschata][more]
KAG6600707.10.0e+0097.84Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023530877.10.0e+0096.35LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pe... [more]
XP_038903187.18.1e-29789.00protein NRT1/ PTR FAMILY 4.3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G59740.18.5e-23675.09Major facilitator superfamily protein [more]
AT1G33440.19.7e-22469.31Major facilitator superfamily protein [more]
AT1G69850.14.8e-11442.02nitrate transporter 1:2 [more]
AT1G27040.21.5e-10740.39Major facilitator superfamily protein [more]
AT1G27040.14.4e-10739.52Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 15..574
e-value: 2.1E-162
score: 543.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 33..569
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 110..533
e-value: 2.8E-90
score: 303.0
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 22..578
NoneNo IPR availablePANTHERPTHR11654:SF551PROTEIN NRT1/ PTR FAMILY 4.3coord: 22..578
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 42..570
e-value: 0.0
score: 654.093

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G008850.1CmaCh04G008850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity