CmaCh04G008550 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G008550
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	SpoU_methylase domain-containing protein
Location	Cma_Chr04: 4381281 .. 4383619 (+)
RNA-Seq Expression	CmaCh04G008550
Synteny	CmaCh04G008550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCCTCGTGTAAATTTTGAGAACAAAAGAAGCCGAGTTTAATTTGAAGAATGCAATGCTCCTGCATTTCCTTGCCCTCAAATTTGAAACTCGGACCTTCCATCTCCAAATTCAAGCTATCATCAAATCTTAAAGTGGAAGCCATTGATCTTGCTGCAAGAATTTCACTTCCTTCTCATGTGAAAGCCATCACCAGCACCTCCAACCCCTTCGTCAAGCATTGCGTTAAACTCCGCTTCAGTTCCTCTTATCGCCACTTCCATGGCTCCGTCCTCGTCGTCGGCGCTACCCCAATTAGGTCGCCTCTTAACGCTTCACTTTCTGCGTCTATCCATAATTTTGTTCTTGAATTGAAAAAACCAAATTCGTCTATGCAGGGTTTTGCCTTTCGAATTCTTTTTGTTGATACATTTCATCAAAAAGGAAACTCTAAATCCTTGAATATTTTTGCACATTTGTCGTATGGGTTAACCAATTCCCTTCCTTCGTTTATTGTTTTTCCCAAGTAAGGATGAAAGTTGTTCTGTGAGTTTCTCCTGAGATGTCGGTTTGATATGAGTAGTTTTATAATTGGCCCATTTTAGTTTCTGAAGAATTAAGTTCTTGGAATCAGGTCTAATGTAACAGCAAGCATGTGACTGAATGTTTTTGAAGCACCCTTAAGCTTCGTCCATAGTTTCATTTGCTTGTGACTGTTCTCTGATTGAGCTATAAAGGATAAATTGTTGGAGTTGTGTACAATATGTGCTTTCCAGTCATTTGAATTCAATCCTTATTAATGTTTTCCCATGAGAAATTTGCTAGCTCCCCCCACCCCCCGGATCTTGGGAATTCTACTTCTGTCTGTATTAGTTCATTTTTCTCAAGCGGTCATTCTTTGAGAGCAATATTTTGCTCGACTTGACGAGGTTTGTATGCACATACATCTAATCTTAGTGTTTTACAGAGAAATATGCAAGTTTCAAGAGTCTCTGCGTGGGATAAGTTGTACACTGGCGTGCCTACTTCTGCTAGATGAAGTCGAGGTTCCTGAAGAACTTGTCGATGTCTCTGTTCGTATCGTGCGGGTGAGCCCAACTGTGATGAAAAAGCTTTCTGGAATGCAATCGATAGAATCAATTGATGCAATTGCCCTCATGAGAATACCTGACAGTTTTTGTAGTTTAGATGGAAACTTAAAACAAGCACATTCTAAAACATGGTTTCCATCTCCACATCGAATACTAGTCCTCGATGGAATCCAGGTAACTATTTCATCTGGAATCGACCTAATCCTGTAATTTGGTGCATTTAGCTGTCCTGTCTCTAAGTTGTTTTAAAATAGTTATCATTTCCTGCATTTCATTCTATAGTTACTGCTGAGATAACTTGGTACTTTGTGTTAAGCTATCTCTCAAAGGCCACTTTTGGTATTTACCTGTGCACATTGACATGGCAATAGTTTCTCTGTAGCTATGGTCTTCTTAATCTATCTTTAAGGCTTTGAAAAGAACTTTTAATGCTTAGCATTTTATTTATAAAGTACTACAGATAATTTCATTGATTTAGTATCGATATGTGAACATGACATTTAAAGGTTCGTTTTCGCTTATTGCAGGACCCAGGCAATCTTGGTACATTAATCAGGTCTGCTTTGGCCTTCAAATGGGTAAGGTTATTGGTTTGAATAATCCTTCTTTGAGCCCAATTTTCAATGAATGCTGATGGACATACTCACCTTGATGGTTCAAAATTACCTGAAGGATTATACATGACATACCGTGTCTAAGACTGCAATTTTCTGAAGTATGATGAAGCATTTGGGTATGTCAAATAAGTTTTCTGATACATATTTGGGTATGTCAAACAGAATGGTGTGTTTCTGCTTCCTGGGTGTTGTGATCCATTCAACGAGAAGGCACTCCGAGCCTCACGAGGAGCATCTTTTCAGCTTCCAATAGTCTCTGGCACTTGGAATCATCTTGAGGCTCTAAAAACTGACTTCCAAATGAAAATGTTGGCTGGACATCCCGAAAGTAATGAACTGAAGCCTGTATCGGTCCTTTCACAACAGTTCGCGAATTCTTCAAAAGATATCTCATTGTGCTTGGTTTTGGGGAGTGAAGGGAGTGGTCTATCTGAGAAATCTTGGCAGGTATGTGAGCTTTTGAGCATTCCAATGGTGGGTGGTTTCGAGTCACTCAACGTCTCAGTTGCAGGAGGAATTTTCTTGTACATGCTGCAGGCTGAAAACGATAGACATATTTGATGTACGATTACCACAACTTGAATTCAAGTTTAATGAAACTTGATTTTAATTAATGTGACTTAGACTTTAATTGGAACGACATTCTTCACATA

mRNA sequence

TGCCTCGTGTAAATTTTGAGAACAAAAGAAGCCGAGTTTAATTTGAAGAATGCAATGCTCCTGCATTTCCTTGCCCTCAAATTTGAAACTCGGACCTTCCATCTCCAAATTCAAGCTATCATCAAATCTTAAAGTGGAAGCCATTGATCTTGCTGCAAGAATTTCACTTCCTTCTCATGTGAAAGCCATCACCAGCACCTCCAACCCCTTCGTCAAGCATTGCGTTAAACTCCGCTTCAGTTCCTCTTATCGCCACTTCCATGGCTCCGTCCTCGTCGTCGGCGCTACCCCAATTAGAGAAATATGCAAGTTTCAAGAGTCTCTGCGTGGGATAAGTTGTACACTGGCGTGCCTACTTCTGCTAGATGAAGTCGAGGTTCCTGAAGAACTTGTCGATGTCTCTGTTCGTATCGTGCGGGTGAGCCCAACTGTGATGAAAAAGCTTTCTGGAATGCAATCGATAGAATCAATTGATGCAATTGCCCTCATGAGAATACCTGACAGTTTTTGTAGTTTAGATGGAAACTTAAAACAAGCACATTCTAAAACATGGTTTCCATCTCCACATCGAATACTAGTCCTCGATGGAATCCAGGACCCAGGCAATCTTGGTACATTAATCAGGTCTGCTTTGGCCTTCAAATGGAATGGTGTGTTTCTGCTTCCTGGGTGTTGTGATCCATTCAACGAGAAGGCACTCCGAGCCTCACGAGGAGCATCTTTTCAGCTTCCAATAGTCTCTGGCACTTGGAATCATCTTGAGGCTCTAAAAACTGACTTCCAAATGAAAATGTTGGCTGGACATCCCGAAAGTAATGAACTGAAGCCTGTATCGGTCCTTTCACAACAGTTCGCGAATTCTTCAAAAGATATCTCATTGTGCTTGGTTTTGGGGAGTGAAGGGAGTGGTCTATCTGAGAAATCTTGGCAGGTATGTGAGCTTTTGAGCATTCCAATGGTGGGTGGTTTCGAGTCACTCAACGTCTCAGTTGCAGGAGGAATTTTCTTGTACATGCTGCAGGCTGAAAACGATAGACATATTTGATGTACGATTACCACAACTTGAATTCAAGTTTAATGAAACTTGATTTTAATTAATGTGACTTAGACTTTAATTGGAACGACATTCTTCACATA

Coding sequence (CDS)

ATGCAATGCTCCTGCATTTCCTTGCCCTCAAATTTGAAACTCGGACCTTCCATCTCCAAATTCAAGCTATCATCAAATCTTAAAGTGGAAGCCATTGATCTTGCTGCAAGAATTTCACTTCCTTCTCATGTGAAAGCCATCACCAGCACCTCCAACCCCTTCGTCAAGCATTGCGTTAAACTCCGCTTCAGTTCCTCTTATCGCCACTTCCATGGCTCCGTCCTCGTCGTCGGCGCTACCCCAATTAGAGAAATATGCAAGTTTCAAGAGTCTCTGCGTGGGATAAGTTGTACACTGGCGTGCCTACTTCTGCTAGATGAAGTCGAGGTTCCTGAAGAACTTGTCGATGTCTCTGTTCGTATCGTGCGGGTGAGCCCAACTGTGATGAAAAAGCTTTCTGGAATGCAATCGATAGAATCAATTGATGCAATTGCCCTCATGAGAATACCTGACAGTTTTTGTAGTTTAGATGGAAACTTAAAACAAGCACATTCTAAAACATGGTTTCCATCTCCACATCGAATACTAGTCCTCGATGGAATCCAGGACCCAGGCAATCTTGGTACATTAATCAGGTCTGCTTTGGCCTTCAAATGGAATGGTGTGTTTCTGCTTCCTGGGTGTTGTGATCCATTCAACGAGAAGGCACTCCGAGCCTCACGAGGAGCATCTTTTCAGCTTCCAATAGTCTCTGGCACTTGGAATCATCTTGAGGCTCTAAAAACTGACTTCCAAATGAAAATGTTGGCTGGACATCCCGAAAGTAATGAACTGAAGCCTGTATCGGTCCTTTCACAACAGTTCGCGAATTCTTCAAAAGATATCTCATTGTGCTTGGTTTTGGGGAGTGAAGGGAGTGGTCTATCTGAGAAATCTTGGCAGGTATGTGAGCTTTTGAGCATTCCAATGGTGGGTGGTTTCGAGTCACTCAACGTCTCAGTTGCAGGAGGAATTTTCTTGTACATGCTGCAGGCTGAAAACGATAGACATATTTGA

Protein sequence

MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVKLRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVRIVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEALKTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLSIPMVGGFESLNVSVAGGIFLYMLQAENDRHI

Homology

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BLAST of CmaCh04G008550 vs. ExPASy Swiss-Prot
Match: P74261 (Uncharacterized tRNA/rRNA methyltransferase slr1673 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1673 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.5e-16
Identity = 88/289 (30.45%), Postives = 136/289 (47.06%), Query Frame = 0


Query: 40  LPSHVKAITSTSNPFVKHCVKLRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTL 99
           L S + +ITST NP VK   +L   +  R   G +L+ G T + E+   Q       C  
Sbjct: 4   LTSDIASITSTQNPLVKQLRQLH-QTKGRKQQGQLLLEG-THLLEVALAQGKGFNHGCFT 63

Query: 100 ACLLLLDEVEVPEELVDVSVRIVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGN 159
           A     + V + + L+  SV    VS  V+ K++   + + + A   M   D F      
Sbjct: 64  AMWQEKNPV-LADRLMAQSVHSYLVSGEVLAKMASTVNPDGVVATLTM---DQF------ 123

Query: 160 LKQAHSKTW-FPSPH-RI-LVLDGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKA 219
                   W  P PH R+ LVL+ +QDPGNLGT++R+A A    G++L   C DP + K 
Sbjct: 124 --------WRSPPPHARLGLVLERLQDPGNLGTILRTAAATGVEGIWLTADCVDPTSPKV 183

Query: 220 LRASRGASFQLPIVSGTWNHLEALKTDFQ---MKMLAGHPESNELKPVSVLSQQFANSSK 279
           LR+S G+S  LP        L  L   F    ++++A  P++ +    ++    F   + 
Sbjct: 184 LRSSAGSSLLLP--QQQLQSLPPLLEKFHTQGLQLIATVPQATQ----TLWEIDFQRPT- 243

Query: 280 DISLCLVLGSEGSGLSEKSWQV-CELLSIPMVGGFESLNVSVAGGIFLY 322
                ++ GSEG GLS    ++    ++IP     ESLNV++A G+ LY
Sbjct: 244 ----IVIFGSEGQGLSAPVLELTTHQVAIPQAPQVESLNVAIAVGVMLY 261

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BLAST of CmaCh04G008550 vs. ExPASy Swiss-Prot
Match: Q99W72 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=SAV0531 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 57/183 (31.15%), Postives = 90/183 (49.18%), Query Frame = 0


Query: 144 IALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDGIQDPGNLGTLIRSALAFKWNGVF 203
           +A +  P  +   D  LKQ   K    +   +L+LDG++DP NLG+++R+A A   +GV 
Sbjct: 69  VAALIAPYEYADFDQFLKQQKEKEGLST---VLILDGLEDPHNLGSILRTADATGVDGVI 128

Query: 204 LLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEALKTDFQMK----MLAGHPESNELK 263
           +         +   +AS GA   +P++  T       KT  ++K     +AG   +N   
Sbjct: 129 IPKRRSVTLTQTVAKASTGAIEHVPVIRVT----NLAKTIDELKDNGFWVAGTEANN--- 188

Query: 264 PVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCEL-LSIPMVGGFESLNVSVAGGI 322
                +  + N   D+SL +V+GSEG G+S      C+  + IPMVG   SLN SVA  +
Sbjct: 189 -----ATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPMVGHVNSLNASVAASL 236

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BLAST of CmaCh04G008550 vs. ExPASy Swiss-Prot
Match: Q7A794 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain N315) OX=158879 GN=SA0490 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 57/183 (31.15%), Postives = 90/183 (49.18%), Query Frame = 0


Query: 144 IALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDGIQDPGNLGTLIRSALAFKWNGVF 203
           +A +  P  +   D  LKQ   K    +   +L+LDG++DP NLG+++R+A A   +GV 
Sbjct: 69  VAALIAPYEYADFDQFLKQQKEKEGLST---VLILDGLEDPHNLGSILRTADATGVDGVI 128

Query: 204 LLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEALKTDFQMK----MLAGHPESNELK 263
           +         +   +AS GA   +P++  T       KT  ++K     +AG   +N   
Sbjct: 129 IPKRRSVTLTQTVAKASTGAIEHVPVIRVT----NLAKTIDELKDNGFWVAGTEANN--- 188

Query: 264 PVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCEL-LSIPMVGGFESLNVSVAGGI 322
                +  + N   D+SL +V+GSEG G+S      C+  + IPMVG   SLN SVA  +
Sbjct: 189 -----ATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPMVGHVNSLNASVAASL 236

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BLAST of CmaCh04G008550 vs. ExPASy Swiss-Prot
Match: Q6GJD7 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=SAR0535 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 57/183 (31.15%), Postives = 90/183 (49.18%), Query Frame = 0


Query: 144 IALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDGIQDPGNLGTLIRSALAFKWNGVF 203
           +A +  P  +   D  LKQ   K    +   +L+LDG++DP NLG+++R+A A   +GV 
Sbjct: 69  VAALIAPYEYADFDQFLKQQKEKEGLST---VLILDGLEDPHNLGSILRTADATGVDGVI 128

Query: 204 LLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEALKTDFQMK----MLAGHPESNELK 263
           +         +   +AS GA   +P++  T       KT  ++K     +AG   +N   
Sbjct: 129 IPKRRSVTLTQTVAKASTGAIEHVPVIRVT----NLAKTIDELKDNGFWVAGTEANN--- 188

Query: 264 PVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCEL-LSIPMVGGFESLNVSVAGGI 322
                +  + N   D+SL +V+GSEG G+S      C+  + IPMVG   SLN SVA  +
Sbjct: 189 -----ATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPMVGHVNSLNASVAASL 236

[Close]

BLAST of CmaCh04G008550 vs. ExPASy Swiss-Prot
Match: Q6GBV6 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=SAS0489 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 57/183 (31.15%), Postives = 90/183 (49.18%), Query Frame = 0


Query: 144 IALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDGIQDPGNLGTLIRSALAFKWNGVF 203
           +A +  P  +   D  LKQ   K    +   +L+LDG++DP NLG+++R+A A   +GV 
Sbjct: 69  VAALIAPYEYADFDQFLKQQKEKEGLST---VLILDGLEDPHNLGSILRTADATGVDGVI 128

Query: 204 LLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEALKTDFQMK----MLAGHPESNELK 263
           +         +   +AS GA   +P++  T       KT  ++K     +AG   +N   
Sbjct: 129 IPKRRSVTLTQTVAKASTGAIEHVPVIRVT----NLAKTIDELKDNGFWVAGTEANN--- 188

Query: 264 PVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCEL-LSIPMVGGFESLNVSVAGGI 322
                +  + N   D+SL +V+GSEG G+S      C+  + IPMVG   SLN SVA  +
Sbjct: 189 -----ATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPMVGHVNSLNASVAASL 236

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BLAST of CmaCh04G008550 vs. ExPASy TrEMBL
Match: A0A6J1JVY6 (uncharacterized protein LOC111488367 OS=Cucurbita maxima OX=3661 GN=LOC111488367 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.0e-186
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR
Sbjct: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG
Sbjct: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS
Sbjct: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 332
           IPMVGGFESLNVSVAGGIFLYMLQAENDRHI
Sbjct: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 331

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BLAST of CmaCh04G008550 vs. ExPASy TrEMBL
Match: A0A6J1FNI0 (uncharacterized protein LOC111447061 OS=Cucurbita moschata OX=3662 GN=LOC111447061 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 9.4e-176
Identity = 313/330 (94.85%), Postives = 318/330 (96.36%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSCISLPSNLKLGPSISKFKLSS  KVEAIDLAARISLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCISLPSNLKLGPSISKFKLSSIPKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRF+SSYRHFHGSVLVVGATPIREICKFQESLRGISCTL CLLLLDEVE+PEELVDVSVR
Sbjct: 61  LRFTSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLECLLLLDEVEIPEELVDVSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           +VRVSPTVMKKLSGMQS ESIDAIALMRIPDSF SLDGNLKQAH K WFPSPHRILVL+G
Sbjct: 121 VVRVSPTVMKKLSGMQSTESIDAIALMRIPDSFYSLDGNLKQAHCKRWFPSPHRILVLEG 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLE L
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEDL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           KT+FQMKMLAGHPESNELKPVSVLSQQFANSSKDI LCLVLGSEGSGLSEK WQVCELLS
Sbjct: 241 KTEFQMKMLAGHPESNELKPVSVLSQQFANSSKDIPLCLVLGSEGSGLSEKCWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRH 331
           IPM GGFESLNVSVAGGIFLYMLQA NDRH
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQAVNDRH 330

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BLAST of CmaCh04G008550 vs. ExPASy TrEMBL
Match: A0A6J1C7F0 (uncharacterized protein LOC111008099 OS=Momordica charantia OX=3673 GN=LOC111008099 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.7e-154
Identity = 282/331 (85.20%), Postives = 295/331 (89.12%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSCI   +NLKLGPSISK K+S     E ID+A RISLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCIFWSTNLKLGPSISKSKVSI-AGAETIDIAGRISLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRHFHGSVLVVGATPIREIC+FQE+L G + TL CLLLLDEV+VPE LVDVSVR
Sbjct: 61  LRFSSSYRHFHGSVLVVGATPIREICRFQETLHGTTSTLECLLLLDEVDVPEGLVDVSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           IVRVSPTVMKKLSGMQS ESIDAIALMRIP SF S+  N K+A+ + WFPS HRILVL+G
Sbjct: 121 IVRVSPTVMKKLSGMQSTESIDAIALMRIPASFSSVYHNEKEANFRRWFPSLHRILVLEG 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           K +FQMKMLAGHPESNE KPVS LSQQFANS KD  LCLVLG EGSGLSEKSWQVCELLS
Sbjct: 241 KNEFQMKMLAGHPESNEPKPVSFLSQQFANSLKDFPLCLVLGCEGSGLSEKSWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 332
           IPM GGFESLNVSVAGGIFLYMLQ EN  HI
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQNENHTHI 330

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BLAST of CmaCh04G008550 vs. ExPASy TrEMBL
Match: A0A5D3DF85 (Putative tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G001150 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.2e-152
Identity = 276/326 (84.66%), Postives = 289/326 (88.65%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSC+S  SN KLGPS  K KLSS  + EAID   RISLPSHVKAI+S SNPFVKHCVK
Sbjct: 1   MQCSCLSWSSNPKLGPSFHKSKLSSINQAEAIDFTGRISLPSHVKAISSISNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRHFHGSV+VVGATPIREICKFQESL GI+CTL  LLLLDEVEVPE LV+++VR
Sbjct: 61  LRFSSSYRHFHGSVVVVGATPIREICKFQESLHGITCTLEYLLLLDEVEVPEGLVNLTVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           IVRVS  VMKKLSG+QS ESIDAIALMRIP SFCS+DGN K    K WFPSPHRILVL+ 
Sbjct: 121 IVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKVVDFKRWFPSPHRILVLER 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLE  
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEVF 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           K +F+MKMLAGHPESNE KPVSVLSQQFANS KD  LCLVLG EGSGLSEKSWQVCELLS
Sbjct: 241 KKEFKMKMLAGHPESNEPKPVSVLSQQFANSLKDFPLCLVLGCEGSGLSEKSWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAE 327
           IPM GGFESLNVSVAGGIFLYMLQAE
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQAE 326

[Close]

BLAST of CmaCh04G008550 vs. ExPASy TrEMBL
Match: A0A1S3BNM2 (uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491842 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.2e-152
Identity = 276/326 (84.66%), Postives = 289/326 (88.65%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSC+S  SN KLGPS  K KLSS  + EAID   RISLPSHVKAI+S SNPFVKHCVK
Sbjct: 1   MQCSCLSWSSNPKLGPSFHKSKLSSINQAEAIDFTGRISLPSHVKAISSISNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRHFHGSV+VVGATPIREICKFQESL GI+CTL  LLLLDEVEVPE LV+++VR
Sbjct: 61  LRFSSSYRHFHGSVVVVGATPIREICKFQESLHGITCTLEYLLLLDEVEVPEGLVNLTVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           IVRVS  VMKKLSG+QS ESIDAIALMRIP SFCS+DGN K    K WFPSPHRILVL+ 
Sbjct: 121 IVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKVVDFKRWFPSPHRILVLER 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLE  
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEVF 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           K +F+MKMLAGHPESNE KPVSVLSQQFANS KD  LCLVLG EGSGLSEKSWQVCELLS
Sbjct: 241 KKEFKMKMLAGHPESNEPKPVSVLSQQFANSLKDFPLCLVLGCEGSGLSEKSWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAE 327
           IPM GGFESLNVSVAGGIFLYMLQAE
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQAE 326

[Close]

BLAST of CmaCh04G008550 vs. NCBI nr
Match: XP_022991844.1 (uncharacterized protein LOC111488367 [Cucurbita maxima])

HSP 1 Score: 661.4 bits (1705), Expect = 4.2e-186
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR
Sbjct: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG
Sbjct: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS
Sbjct: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 332
           IPMVGGFESLNVSVAGGIFLYMLQAENDRHI
Sbjct: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 331

[Close]

BLAST of CmaCh04G008550 vs. NCBI nr
Match: XP_023545415.1 (uncharacterized protein LOC111804847 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633.3 bits (1632), Expect = 1.2e-177
Identity = 314/331 (94.86%), Postives = 322/331 (97.28%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSCISLPSNLKLGPS+SKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCISLPSNLKLGPSVSKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRHFHGSV+VVGATPIREICKFQE+LRGISCTL CLLLLDEVE+PEELVDVSVR
Sbjct: 61  LRFSSSYRHFHGSVIVVGATPIREICKFQETLRGISCTLECLLLLDEVEIPEELVDVSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           +V+VSPTVMKKLSGMQS ESIDAIALMRIPDSF SLDGNLKQAH K WFPSPHRILVL+G
Sbjct: 121 VVQVSPTVMKKLSGMQSTESIDAIALMRIPDSFYSLDGNLKQAHCKRWFPSPHRILVLEG 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           KT+FQMKMLAGHPESNELKPVSVLSQQFANSSKDI LCLVLGSEGSGLSEK WQVCELLS
Sbjct: 241 KTEFQMKMLAGHPESNELKPVSVLSQQFANSSKDIPLCLVLGSEGSGLSEKCWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 332
           IPM GGFESLNVSVAGGIFLYMLQA NDRHI
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQAVNDRHI 331

[Close]

BLAST of CmaCh04G008550 vs. NCBI nr
Match: XP_022941807.1 (uncharacterized protein LOC111447061 [Cucurbita moschata])

HSP 1 Score: 625.9 bits (1613), Expect = 1.9e-175
Identity = 313/330 (94.85%), Postives = 318/330 (96.36%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSCISLPSNLKLGPSISKFKLSS  KVEAIDLAARISLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCISLPSNLKLGPSISKFKLSSIPKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRF+SSYRHFHGSVLVVGATPIREICKFQESLRGISCTL CLLLLDEVE+PEELVDVSVR
Sbjct: 61  LRFTSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLECLLLLDEVEIPEELVDVSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           +VRVSPTVMKKLSGMQS ESIDAIALMRIPDSF SLDGNLKQAH K WFPSPHRILVL+G
Sbjct: 121 VVRVSPTVMKKLSGMQSTESIDAIALMRIPDSFYSLDGNLKQAHCKRWFPSPHRILVLEG 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLE L
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEDL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           KT+FQMKMLAGHPESNELKPVSVLSQQFANSSKDI LCLVLGSEGSGLSEK WQVCELLS
Sbjct: 241 KTEFQMKMLAGHPESNELKPVSVLSQQFANSSKDIPLCLVLGSEGSGLSEKCWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRH 331
           IPM GGFESLNVSVAGGIFLYMLQA NDRH
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQAVNDRH 330

[Close]

BLAST of CmaCh04G008550 vs. NCBI nr
Match: KAG6600682.1 (hypothetical protein SDJN03_05915, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 624.0 bits (1608), Expect = 7.3e-175
Identity = 313/331 (94.56%), Postives = 318/331 (96.07%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSCISLPSNLKLGPSISKFKLSS  KVEAIDLAARISLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCISLPSNLKLGPSISKFKLSSIPKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTL CLLLLDEVE+PEELVDVSVR
Sbjct: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLECLLLLDEVEIPEELVDVSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           +VRVS TVMKKLSGMQS ESIDAIALMRIPDSF SLDGNLKQAH K WFPSPHRILVL+G
Sbjct: 121 VVRVSSTVMKKLSGMQSTESIDAIALMRIPDSFYSLDGNLKQAHCKRWFPSPHRILVLEG 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           KT+FQM+MLAGHPESNELKPVSVLSQQFANS KDI LCLVLGSEGSGLSEK WQVCELLS
Sbjct: 241 KTEFQMEMLAGHPESNELKPVSVLSQQFANSWKDIPLCLVLGSEGSGLSEKCWQVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 332
           IPM GGFESLNVSVAGGIFLYMLQA NDRHI
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQAANDRHI 331

[Close]

BLAST of CmaCh04G008550 vs. NCBI nr
Match: XP_038904874.1 (uncharacterized tRNA/rRNA methyltransferase slr1673 [Benincasa hispida])

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-155
Identity = 282/331 (85.20%), Postives = 295/331 (89.12%), Query Frame = 0


Query: 1   MQCSCISLPSNLKLGPSISKFKLSSNLKVEAIDLAARISLPSHVKAITSTSNPFVKHCVK 60
           MQCSC+S  SNLKLGPS SK KLSS  + E ID AAR+SLPSHVKAITSTSNPFVKHCVK
Sbjct: 1   MQCSCVSWSSNLKLGPSFSKSKLSSINQAETIDFAARVSLPSHVKAITSTSNPFVKHCVK 60

Query: 61  LRFSSSYRHFHGSVLVVGATPIREICKFQESLRGISCTLACLLLLDEVEVPEELVDVSVR 120
           LRFSSSYRH+HG+VLVVGATPIREICKFQESL GI+CTL CLLLLD+VEVPE LVD SVR
Sbjct: 61  LRFSSSYRHYHGTVLVVGATPIREICKFQESLHGITCTLECLLLLDDVEVPEGLVDKSVR 120

Query: 121 IVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFCSLDGNLKQAHSKTWFPSPHRILVLDG 180
           IVRVSPTVM+KLSGMQS ESIDAIALMRIP SFCS+DGN K    K  FPSPHRILVL+ 
Sbjct: 121 IVRVSPTVMRKLSGMQSTESIDAIALMRIPASFCSVDGNEKDIDFKRCFPSPHRILVLER 180

Query: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWNHLEAL 240
           IQDPGNLGTLIRSALAFKWNGVFLLPGCCDP+NEKALRASRGASFQLPIVSGTWNHLE L
Sbjct: 181 IQDPGNLGTLIRSALAFKWNGVFLLPGCCDPYNEKALRASRGASFQLPIVSGTWNHLEVL 240

Query: 241 KTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQVCELLS 300
           K  FQMKMLAGHPESNE K VSVLSQQFANS K+  LCLVLG EGSGLS KSW VCELLS
Sbjct: 241 KNKFQMKMLAGHPESNEPKRVSVLSQQFANSLKEFPLCLVLGCEGSGLSGKSWHVCELLS 300

Query: 301 IPMVGGFESLNVSVAGGIFLYMLQAENDRHI 332
           IPM GGFESLNVSVAGGIFLYMLQAEN+ HI
Sbjct: 301 IPMAGGFESLNVSVAGGIFLYMLQAENNSHI 331

[Close]

BLAST of CmaCh04G008550 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 399.4 bits (1025), Expect = 2.7e-111
Identity = 198/293 (67.58%), Postives = 237/293 (80.89%), Query Frame = 0


Query: 35  AARISLPSHVKAITSTSNPFVKHCVKLRFSSSYRHFHGSVLVVGATPIREICKFQESLRG 94
           A R+SLPSHV +ITST+NPFVKHC+KLR SSSYRH HGSVLVVG  PIRE+C FQ + +G
Sbjct: 57  ANRVSLPSHVNSITSTTNPFVKHCLKLRQSSSYRHAHGSVLVVGTIPIREVCMFQTNKQG 116

Query: 95  ISCTLACLLLLDEVEVPEELVDVSVRIVRVSPTVMKKLSGMQSIESIDAIALMRIPDSFC 154
           ++  + CLLL +E ++P+ L  +S+RIVRVS  VMKKLSG+QS ES++AIALMRIP SF 
Sbjct: 117 MTTEIECLLLHEEAKIPQGLESLSIRIVRVSSLVMKKLSGVQSTESVEAIALMRIPSSFT 176

Query: 155 SL-DGNLKQAHSKTWFPSPHRILVLDGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFN 214
            L D          WFPS HR+LVLD IQDPGNLGTL+RSA+AF W+G FLLPGCCDP+N
Sbjct: 177 DLKDDKDIITDCNKWFPSAHRVLVLDSIQDPGNLGTLVRSAMAFNWDGAFLLPGCCDPYN 236

Query: 215 EKALRASRGASFQLPIVSGTWNHLEALKTDFQMKMLAGHP--ESNELKPVSVLSQQFANS 274
           +KALRASRGASFQLPIVSG WNHL+ L+ +FQMK+LAGHP   + +LKPVS LS +FA S
Sbjct: 237 DKALRASRGASFQLPIVSGNWNHLKLLENEFQMKLLAGHPATTTQKLKPVSKLSVEFAQS 296

Query: 275 SKDISLCLVLGSEGSGLSEKSWQVCELLSIPMVGGFESLNVSVAGGIFLYMLQ 325
             +  LCL+LGSEG+GLSE++ +VC L+SIPM G FESLNVSVAGGIFLYMLQ
Sbjct: 297 LAEKPLCLILGSEGNGLSEQARKVCVLVSIPMAGDFESLNVSVAGGIFLYMLQ 349

[Close]

BLAST of CmaCh04G008550 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.4e-06
Identity = 44/156 (28.21%), Postives = 75/156 (48.08%), Query Frame = 0


Query: 176 LVLDGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGASFQLPIVSGTWN 235
           LV++G+ D GN+    RSA A     V ++   CD  + K    +R  S    + +  W 
Sbjct: 157 LVVEGLSDFGNISAAFRSADALGIQSVHVV--SCD--SSKRYNGNRHVS----MGAEKWL 216

Query: 236 HLEALKTDFQ-MKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLSEKSWQ 295
            +E   T  +  K+L           + + +    +        +V+G+EG G+S+++ +
Sbjct: 217 DIEFWDTPKECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALE 276

Query: 296 VCEL-LSIPMVGGFESLNVSVAGGIFLYMLQAENDR 330
           + +L  SIPM G  +S NVSVA GI ++   A +DR
Sbjct: 277 LSDLRCSIPMNGMVDSFNVSVAAGILMH--HAVSDR 302

[Close]

BLAST of CmaCh04G008550 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 49.3 bits (116), Expect = 7.0e-06
Identity = 53/172 (30.81%), Postives = 74/172 (43.02%), Query Frame = 0


Query: 176 LVLDGIQDPGNLGTLIRSALAFKWNGVFLLPGCCDPFNEKALRASRGA--SFQLPIVSGT 235
           + LD + DP NLG +IRSA  F   GV +      P +    +AS G+    +L      
Sbjct: 420 VALDEVTDPQNLGAIIRSAYFFGATGVVVCAKNSAPLSAVVSKASAGSLEVMELRYCKNM 479

Query: 236 WNHLEALKTDFQMKMLAGHPESNELKPVSVLSQQFANSSKDISLCLVLGSEGSGLS---E 295
              LEA   +   +++ G      + P +V   +    S  I   LVLG+EG+GL    E
Sbjct: 480 MQFLEA-SAENGWRVVGG-----SVSPKAVALNEVLPGSPTI---LVLGNEGTGLRPLVE 539

Query: 296 KSWQVCELLSIPMVG-------------GF------ESLNVSVAGGIFLYML 324
           +S      +S  M               GF      ESLNVSVA G+FL+ L
Sbjct: 540 RSCTDLVRISGNMPNEVAVTESDDAEGEGFRSFLAVESLNVSVAAGLFLHHL 582

The following BLAST results are available for this feature:

BLAST of CmaCh04G008550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P74261	1.5e-16	30.45	Uncharacterized tRNA/rRNA methyltransferase slr1673 OS=Synechocystis sp. (strain...	[more]
Q99W72	2.1e-15	31.15	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q7A794	2.1e-15	31.15	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q6GJD7	2.1e-15	31.15	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q6GBV6	2.1e-15	31.15	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]

BLAST of CmaCh04G008550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JVY6	2.0e-186	100.00	uncharacterized protein LOC111488367 OS=Cucurbita maxima OX=3661 GN=LOC111488367...	[more]
A0A6J1FNI0	9.4e-176	94.85	uncharacterized protein LOC111447061 OS=Cucurbita moschata OX=3662 GN=LOC1114470...	[more]
A0A6J1C7F0	7.7e-154	85.20	uncharacterized protein LOC111008099 OS=Momordica charantia OX=3673 GN=LOC111008...	[more]
A0A5D3DF85	4.2e-152	84.66	Putative tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo var. makuwa...	[more]
A0A1S3BNM2	4.2e-152	84.66	uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1 OS=Cucumis melo OX=3...	[more]

BLAST of CmaCh04G008550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022991844.1	4.2e-186	100.00	uncharacterized protein LOC111488367 [Cucurbita maxima]	[more]
XP_023545415.1	1.2e-177	94.86	uncharacterized protein LOC111804847 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022941807.1	1.9e-175	94.85	uncharacterized protein LOC111447061 [Cucurbita moschata]	[more]
KAG6600682.1	7.3e-175	94.56	hypothetical protein SDJN03_05915, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038904874.1	1.3e-155	85.20	uncharacterized tRNA/rRNA methyltransferase slr1673 [Benincasa hispida]	[more]

BLAST of CmaCh04G008550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38020.1	2.7e-111	67.58	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT5G15390.1	2.4e-06	28.21	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT2G19870.1	7.0e-06	30.81	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029064	50S ribosomal protein L30e-like	GENE3D	3.30.1330.30		coord: 46..150 e-value: 4.2E-11 score: 45.0
IPR029064	50S ribosomal protein L30e-like	SUPERFAMILY	55315	L30e-like	coord: 46..141
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 175..321 e-value: 2.9E-29 score: 102.1
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 165..330 e-value: 5.7E-40 score: 138.6
None	No IPR available	PANTHER	PTHR43191	RRNA METHYLTRANSFERASE 3,	coord: 44..326
None	No IPR available	PANTHER	PTHR43191:SF2	RRNA METHYLTRANSFERASE 3, MITOCHONDRIAL	coord: 44..326
None	No IPR available	CDD	cd18095	SpoU-like_rRNA-MTase	coord: 174..323 e-value: 2.26491E-53 score: 170.3
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 168..323

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G008550.1	CmaCh04G008550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001510	RNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity