CmaCh04G006050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G006050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description9-cis-epoxycarotenoid dioxygenase, putative
LocationCma_Chr04: 3075614 .. 3081929 (-)
RNA-Seq ExpressionCmaCh04G006050
SyntenyCmaCh04G006050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCCCTTTCTTCCCCTTTCCTCACCGGCGGGAACCTCCTCCTCTCTCCGCCAATCTCCATCTCCCGACCGCCCTTTTCTATGGCCATAACATCCGTTTTTACCGAAGAAACCCCCAAAACCGTGAAAAAAACGAATGCGGATTCTCCATCGCCCCGACGACCTCCTCCTCCTGCAATGGCGAAGGGCCCTTCCACCACACGACTCGAACCATCTTTACCGGCGAGGTTTTTCAATGCCTTTGACGACCTGATCAACAACTTCATAAACCCGCCGGTCAGTCCGTCGGTCGATCCACGGTACGTCCTTGCCGACAACTTCGCTCCGGTGGACGAGTTACCACCGACGGAGTGTGAAGTCATCCAAGGCTCGTTGGCTTCGTCTCTCAACGGCGCGTACATTCGTAACGGCCCGAATCCTCAGTACCTTCCCCGTGGGCCCTACCATTTGTTCGACGGCGACGGGATGCTTCACTCGCTTCGAATCTCCAACGGCCAAGCCGTTCTGTGCAGTCGTTATGTCAAGACTTACTGTGAATGTTTGATTAATCCACCACACCCAAATGGTGTGAGACTTCTCTTATTTATAATCATTTACAGAATACAGAAATAAGGTAAATTACAAATAATAAGGAAATAGCGATAAAAGGAAAGAATAATAAATGAATACAATATATTTGCATTTAGTAAAAGGGAAAATATGGTAACCCGTAATGTAAGGCAAATATCTAGATATTTGCCTTATAATGAACGGTCAACTATCCTTAACACCCCCCCGCAAGCTGAGCGTCGGATTTGAACGAACATGAAGCTTGGATCTGAAAAATTCAAAGAGAGGTCGAGAAATACTCTTTGTGAAGATGTCAGCAACTTGGAGATGAGAGGGTACATATTGTGTACGAAGTTTGCCAGCGATAACAAGTTCTCGAAGGAAATGATAATCTAGTTCAACATGCTTGGCCCGCTTGTGAGAAACAGGATTAGAGCTCAAAAAAATAGCACTTTTGTTGTCACATAAGAGTAAGGGCTGCTGTGAAATAGGGACCTTGAGGTCATGCAAAAGATGCGTAACCCAAAGAAGTTCAGCAGCAGTTGTGGCAAGAGCACGATACTCAGATTCACAGCTGGAGCGTGATACAGTAGGTTGCTTTTTGGCACTCCAAGAAATGAGATTGTTACCAAGATAAATAGAATAGCCGGATGTAGAACGACGAGTATCGGGACAACCAGCCCAGTCAGCATCCGAATAAGCGACTAGCGTACTAGGAACAGTGGATGGACGAAAGATAAGACCAAAGTGGAGTGTTCCTTTGACATAGCGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAACGAGGAGAAAGATGAGAGTGAAAGAGAAGGGCCAAGCCAGTCTCAGTCACATGACGATGTTTTCTCTCAACACGACCATTTTGAGCAGGTGTATATGGACAAGAGAGTTGATGGTGGATGCCGAGTCCACGAAGGAACCAATGCACTTTATCAATGTCCTCGACGGGTCTGCCAATGGCATGAAGTTGGTCACAAATTTTTTTGAAGGCACGGGCATACTCAGCAACAGGTTTTGTGCCACGTTTNTATTGATTTTCCACAAATTTTTGAAATTGAAGAAAAATATCAAAAAAATCAGATTTAAATTTTAAAGGGTAAAACCAAGTGAATCGAGAATAATCATCAATAAAAATAGCATAATAAAGAAAACCTGAATTTGATTTGATGGGAGAAGGACCCCAAAGATCACAATGAATAAGATCTAACACATGAGACGACCTACGTTCATTGCGGGAATAAGGCAATCGATGGCTTTTCGCAAGCTGACAACTATTACATAATGATGGAGAAGGCAATAAAGACGTAAGAGAAAGATGACCTTTTTTATTTAAAAAAGAAATAACAGAATGATTCACATGACCCAGACGAGCATGCCATAAATCATATGAAGCACGTAAAGATTTGTTTTTAAGGGCTGAAATAAAAGCAGAGTTGCCGCGCTCCAGCACATATAGCCCTCCATCTCTTTTACCGGTTGCCACCACCCTTCCTGTTTGATGATTCTGGATAGTAATAAGATTATTAGTAAATGTAACGGAGAGAGGAAAATCAGACGTTAATTTACTTATGGAAAGAAGATTTTTAGTGAGGTGAGGGACAACCAAGACATCTAATAAGTGAATATTTGGAACAGGAGAAAGAGTACCGATGTGGGTAATGGGTAGGGATGCACCGTTTCCTACAATCACAGAGTCCTTACCCGTGTAATTTTTAGACTGATCCAGAATTGATGGGTCGGCAGTCATATGGGCCGAAGCTCCAGTGTCCAAAAACCAATCAGCAGCATCGGGTCCAGCAATAGAACATGACGTGTTAAAGGCTTCAGCAAGGTGAGCATGAGAAGAATCAGGTCGAACATACCGTTGGTTGCAGCGGTCAGCATAATGGCCCTCTTTGCGGCATATTTGGCAATGAGGTGGTCGACGACCCTGACTTGAGTGGGTTCGTCCTCGATTAGAAGAGTTGTTTTTGTGAGAATAAGAACGACCTCGCTGGTTGGTAAAGGAAGCAGGGTGACTTTCATGGGTGCGACCACGATTAGTGGCTATGAATGTTGTAGGAGTGGAGTCAGAGGACTCAAGGGAGCGCTGGAACAACTCAAAACTTTCAGTTTTAGAGACTAGATCTGCAAAACAGGGGATAGGGGTGAGAGCCATCTGAGCAGTAGAAAAAGCTGAAAATTCGGTGCCGAGTCCACGAAGGAACCAGTGCACTTTATCAATGTCCTCGACGGGTCTGCCAATGGCATGAAGTTGGTCACAAATTTTTTTGAAGGCACGGGCATACTCAGCAACAGGTTTTGTGCCACGTTTCATCAACTGCAAGTCATCCTTGAGTCTCAGTTCACGAGCTTTTGACTGATGGCTAAACGTAGTTTCCAACGCAAGCCAAACATCACGTGCAGTAGAGAGACCAACGACAACAGCCATGGCTTCCTCAGTGAGAGAGGAGAGCAGGAGACAGAGAAGTCGTTGATCGGCTGCTCTCCATGCCAAATATTTGGGGTTGAATGTTGAGGAGGTTTCTGGTTCAAAGCGAGGTGGTGGAACCATAGTTCCATCGACATAGCCCAGCATGTCTTGACTCTCAAGAAGAGGGAGAAGTTGGCTTTTCCAAAGAAGATAATTGGAGGAAGAAAGTTTGATGGTGATCATATGGATTAGAGTATTGAAAGGAAGAAGATGATAACAAGATTCGGAAGCCATAGAGAAGAAAGGATCAGGGTTGTTAAACCGAAAGGGCTCTGATACCATGTGAATGTTTGATTAATCCACCACACCCAAATGGTGTGAGACTTCTCTTATTTATAATCATTTACAGAATACAGAAATAAGGTAAATTACAAATAATAAGGAAATAGCGATAAAAGGAAAGAATAATAAATGAATACAATATATTTGCATTTAGTAAAAGGGAAAATATGGTAACCCGTAATGTAAGGCAAATATCTAGATATTTGCCTTATAATGAACGGTCAACTATCCTTAACACTTACAAATACATTCTGGAGCGTGATGCTGGTCATCCTGTTTTCCCCAATGTTTTCTCCGGCTTCAATGGACTCACAGCCTCCGCCGCGCGGGGCGCCGTCGCAGCTGGCCGGGTGTTAACCGGCCAGTACAATCCGGCGAACGGCATTGGCCTTGCCAACACCAGTGTCGCTTTCTTTGGCGACCGCCTTTACGCCCTAGGTGAGTCCGATTTACCGTATTCGATCCGATTGGCTCCGAACGGCGAAATTGAAACTCTCGGCCGCCAGGATTTCGACGGAAATCTGTTTATGAGTATGACGGCTCATCCGAAATTCGATCCCGATACTGGTGAAACATTCGCGTTTCGGTACGGTCCTCTGCCTCCGTTTTTAACCTTCTTTCGATTCGACAAAAACGGAGCCAAACAGTCCGATGTACCGATATTTTCGATGAATCGACCTTCCTTCCTGCACGATTTTGCAATTACGAAAAAGTACGCTGTATTTACAGAGATTCAGATTGGATTTAATCCTATGCTGATGATAATGGAAGGACGATCTCCAGTTGGAACAGATCCATCGACAGTTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGACGAATCGAAGATGGAGTGGTTCGATGTACCTGGATTTAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGGTTCTGCTGGCGCCGAACATTCTCTCCGTCGAACACACACTGGAGAGGATGGATCTGGTTCACGCCTTAATGGAGGAGGTCAGAATCGACTTGAAAACAGGAATAGTTACGCGGCGGCCATTGTCTACCAGAAACTTAGACTTTGGAGTGATAAATCCGTCGTACATCGGGAAGAAAAACAAGTTCGTATACGCCGGCGTTGGAGATCCAATGCCTAAGATATCGGGAGTAGTAAAGCTCGACGTGTCTCAAGAAGAACGCCGAGACTGCATCGTTGCGAGTAGGATTTTCGGGCCTGGTTGCTACGGCGGCGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAGTTCCGACAAGACGGCGTTGGAGGAAGACGACGGATATGTTGTCTCGTACGTTCACGACGAAAACTTCGGCGAGTCGAAGTTCATCGTCATGGACGCCAAGTCGCCGAAGCTCGAGATTATCGCTGTCGTGAAGCTGCCGCGTCGGGTCCCTTACGGCTTCCACGGATTGTTTGTGAAAGAAAGTGATCTCAATATGCTGTGA

mRNA sequence

ATGGACGCCCTTTCTTCCCCTTTCCTCACCGGCGGGAACCTCCTCCTCTCTCCGCCAATCTCCATCTCCCGACCGCCCTTTTCTATGGCCATAACATCCGTTTTTACCGAAGAAACCCCCAAAACCGTGAAAAAAACGAATGCGGATTCTCCATCGCCCCGACGACCTCCTCCTCCTGCAATGGCGAAGGGCCCTTCCACCACACGACTCGAACCATCTTTACCGGCGAGGTTTTTCAATGCCTTTGACGACCTGATCAACAACTTCATAAACCCGCCGGTCAGTCCGTCGGTCGATCCACGGTACGTCCTTGCCGACAACTTCGCTCCGGTGGACGAGTTACCACCGACGGAGTGTGAAGTCATCCAAGGCTCGTTGGCTTCGTCTCTCAACGGCGCGTACATTCGTAACGGCCCGAATCCTCAGTACCTTCCCCCCTCCGCCGCGCGGGGCGCCGTCGCAGCTGGCCGGGTGTTAACCGGCCAGTACAATCCGGCGAACGGCATTGGCCTTGCCAACACCAGTGTCGCTTTCTTTGGCGACCGCCTTTACGCCCTAGGTGAGTCCGATTTACCGTATTCGATCCGATTGGCTCCGAACGGCGAAATTGAAACTCTCGGCCGCCAGGATTTCGACGGAAATCTGTTTATGAGTATGACGGCTCATCCGAAATTCGATCCCGATACTGGTGAAACATTCGCGTTTCGGTACGGTCCTCTGCCTCCGTTTTTAACCTTCTTTCGATTCGACAAAAACGGAGCCAAACAGTCCGATGTACCGATATTTTCGATGAATCGACCTTCCTTCCTGCACGATTTTGCAATTACGAAAAAGTACGCTGTATTTACAGAGATTCAGATTGGATTTAATCCTATGCTGATGATAATGGAAGGACGATCTCCAGTTGGAACAGATCCATCGACAGTTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGACGAATCGAAGATGGAGTGGTTCGATGTACCTGGATTTAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGGTTCTGCTGGCGCCGAACATTCTCTCCGTCGAACACACACTGGAGAGGATGGATCTGGTTCACGCCTTAATGGAGGAGGTCAGAATCGACTTGAAAACAGGAATAGTTACGCGGCGGCCATTGTCTACCAGAAACTTAGACTTTGGAGTGATAAATCCGTCGTACATCGGGAAGAAAAACAAGTTCGTATACGCCGGCGTTGGAGATCCAATGCCTAAGATATCGGGAGTAGTAAAGCTCGACGTGTCTCAAGAAGAACGCCGAGACTGCATCGTTGCGAGTAGGATTTTCGGGCCTGGTTGCTACGGCGGCGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAGTTCCGACAAGACGGCGTTGGAGGAAGACGACGGATATGTTGTCTCGTACGTTCACGACGAAAACTTCGGCGAGTCGAAGTTCATCGTCATGGACGCCAAGTCGCCGAAGCTCGAGATTATCGCTGTCGTGAAGCTGCCGCGTCGGGTCCCTTACGGCTTCCACGGATTGTTTGTGAAAGAAAGTGATCTCAATATGCTGTGA

Coding sequence (CDS)

ATGGACGCCCTTTCTTCCCCTTTCCTCACCGGCGGGAACCTCCTCCTCTCTCCGCCAATCTCCATCTCCCGACCGCCCTTTTCTATGGCCATAACATCCGTTTTTACCGAAGAAACCCCCAAAACCGTGAAAAAAACGAATGCGGATTCTCCATCGCCCCGACGACCTCCTCCTCCTGCAATGGCGAAGGGCCCTTCCACCACACGACTCGAACCATCTTTACCGGCGAGGTTTTTCAATGCCTTTGACGACCTGATCAACAACTTCATAAACCCGCCGGTCAGTCCGTCGGTCGATCCACGGTACGTCCTTGCCGACAACTTCGCTCCGGTGGACGAGTTACCACCGACGGAGTGTGAAGTCATCCAAGGCTCGTTGGCTTCGTCTCTCAACGGCGCGTACATTCGTAACGGCCCGAATCCTCAGTACCTTCCCCCCTCCGCCGCGCGGGGCGCCGTCGCAGCTGGCCGGGTGTTAACCGGCCAGTACAATCCGGCGAACGGCATTGGCCTTGCCAACACCAGTGTCGCTTTCTTTGGCGACCGCCTTTACGCCCTAGGTGAGTCCGATTTACCGTATTCGATCCGATTGGCTCCGAACGGCGAAATTGAAACTCTCGGCCGCCAGGATTTCGACGGAAATCTGTTTATGAGTATGACGGCTCATCCGAAATTCGATCCCGATACTGGTGAAACATTCGCGTTTCGGTACGGTCCTCTGCCTCCGTTTTTAACCTTCTTTCGATTCGACAAAAACGGAGCCAAACAGTCCGATGTACCGATATTTTCGATGAATCGACCTTCCTTCCTGCACGATTTTGCAATTACGAAAAAGTACGCTGTATTTACAGAGATTCAGATTGGATTTAATCCTATGCTGATGATAATGGAAGGACGATCTCCAGTTGGAACAGATCCATCGACAGTTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGACGAATCGAAGATGGAGTGGTTCGATGTACCTGGATTTAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGGTTCTGCTGGCGCCGAACATTCTCTCCGTCGAACACACACTGGAGAGGATGGATCTGGTTCACGCCTTAATGGAGGAGGTCAGAATCGACTTGAAAACAGGAATAGTTACGCGGCGGCCATTGTCTACCAGAAACTTAGACTTTGGAGTGATAAATCCGTCGTACATCGGGAAGAAAAACAAGTTCGTATACGCCGGCGTTGGAGATCCAATGCCTAAGATATCGGGAGTAGTAAAGCTCGACGTGTCTCAAGAAGAACGCCGAGACTGCATCGTTGCGAGTAGGATTTTCGGGCCTGGTTGCTACGGCGGCGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAGTTCCGACAAGACGGCGTTGGAGGAAGACGACGGATATGTTGTCTCGTACGTTCACGACGAAAACTTCGGCGAGTCGAAGTTCATCGTCATGGACGCCAAGTCGCCGAAGCTCGAGATTATCGCTGTCGTGAAGCTGCCGCGTCGGGTCCCTTACGGCTTCCACGGATTGTTTGTGAAAGAAAGTGATCTCAATATGCTGTGA

Protein sequence

MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLASSLNGAYIRNGPNPQYLPPSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLERMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDENFGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKESDLNML
Homology
BLAST of CmaCh04G006050 vs. ExPASy Swiss-Prot
Match: O49675 (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD4 PE=1 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 4.3e-188
Identity = 339/577 (58.75%), Postives = 407/577 (70.54%), Query Frame = 0

Query: 23  SRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPP--------AMAKGPSTTRLEPSL 82
           S  P  + I S   EE       TN    + RR  P          +   P   R E +L
Sbjct: 25  SSSPTLLRINSAVVEERSPI---TNPSDNNDRRNKPKTLHNRTNHTLVSSPPKLRPEMTL 84

Query: 83  PARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPV-DELPPTECEVIQGSLASSLNGA 142
               F   +D+IN FI+PP  PSVDP++VL+DNFAPV DELPPT+CE+I G+L  SLNGA
Sbjct: 85  ATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSLNGA 144

Query: 143 YIRNGPNPQYLP------------------------------------------------ 202
           YIRNGPNPQ+LP                                                
Sbjct: 145 YIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEKQTGAPVMPN 204

Query: 203 ---------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIR 262
                     S ARGA+ A RVLTGQYNP NGIGLANTS+AFF +RL+ALGESDLPY++R
Sbjct: 205 VFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALGESDLPYAVR 264

Query: 263 LAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQ 322
           L  +G+IET+GR DFDG L MSMTAHPK DP TGETFAFRYGP+PPFLT+FRFD  G KQ
Sbjct: 265 LTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYFRFDSAGKKQ 324

Query: 323 SDVPIFSMNRPSFLHDFAITKKYAVFTEIQIG--FNPMLMIMEGRSPVGTDPSTVSRVGL 382
            DVPIFSM  PSFLHDFAITK++A+F EIQ+G   N + +++EG SPVGTD     R+G+
Sbjct: 325 RDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGKTPRLGV 384

Query: 383 IPRYANDESKMEWFDVPGFNLIHAINAWDEDD--AVVLLAPNILSVEHTLERMDLVHALM 442
           IP+YA DES+M+WF+VPGFN+IHAINAWDEDD  +VVL+APNI+S+EHTLERMDLVHAL+
Sbjct: 385 IPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMSIEHTLERMDLVHALV 444

Query: 443 EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKISGVVKLDVSQ 502
           E+V+IDL TGIV R P+S RNLDF VINP+++G+ +++VYA +GDPMPKISGVVKLDVS+
Sbjct: 445 EKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKISGVVKLDVSK 504

Query: 503 EERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDENFGESKFIV 530
            +R DC VA R++G GCYGGEPFFV R+  + +    EEDDGYVV+YVHDE  GESKF+V
Sbjct: 505 GDRDDCTVARRMYGSGCYGGEPFFVARDPGNPE---AEEDDGYVVTYVHDEVTGESKFLV 564

BLAST of CmaCh04G006050 vs. ExPASy Swiss-Prot
Match: Q6YVJ0 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 1.2e-161
Identity = 306/587 (52.13%), Postives = 387/587 (65.93%), Query Frame = 0

Query: 14  LLLSPPISISRPPFSMAITS---VFTEETPKTVKKTN----ADSPS-PRRPPPPAMAKGP 73
           +L SP ++ +R P          V   +T +TV  T     A S S PRR P PA A   
Sbjct: 57  VLTSPLVTTTRTPKQTEQEDEQLVAKTKTTRTVIATTNGRAAPSQSRPRRRPAPAAAASA 116

Query: 74  STTRLEPSLPARFFNAFDDLINNFINPP-VSPSVDPRYVLADNFAPVDELPPTECEVIQG 133
           +      SLP  F NA +++IN FI+PP + P+VDPR VL  NF PVDELPPT C V++G
Sbjct: 117 A------SLPMTFCNALEEVINTFIDPPALRPAVDPRNVLTSNFVPVDELPPTPCPVVRG 176

Query: 134 SLASSL-NGAYIRNGPNPQYLP------------------PS------------------ 193
           ++   L  GAYIRNGPNPQ+LP                  PS                  
Sbjct: 177 AIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSPASSGDDPVLCSRYVQTY 236

Query: 194 -------------------------AARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDR 253
                                     ARGAV A RVLTGQ NP  G+GLANTS+A+F  R
Sbjct: 237 KYLVERDAGAPVLPNVFSGFHGVAGMARGAVVAARVLTGQMNPLEGVGLANTSLAYFAGR 296

Query: 254 LYALGESDLPYSIRLAPN-GEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLP 313
           LYALGESDLPY++R+ P+ GE+ T GR DF G L M MTAHPK DP TGE FAFRYGP+P
Sbjct: 297 LYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPKKDPVTGELFAFRYGPVP 356

Query: 314 PFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSP 373
           PF+T+FRFD  G K +DVPIFS+ +PSFLHDFAIT++YA+F EIQI   PM M++ G SP
Sbjct: 357 PFVTYFRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPEIQIVMKPMDMVVGGGSP 416

Query: 374 VGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDE--DDAVVLLAPNILSVE 433
           VG+DP  V R+G+IPRYA DES+M WF+VPGFN++H++NAW+E   + +VL+APN+LS+E
Sbjct: 417 VGSDPGKVPRLGVIPRYATDESEMRWFEVPGFNIMHSVNAWEEAGGEELVLVAPNVLSIE 476

Query: 434 HTLERMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPM 493
           H LE M+LVH+ +E+VRI+L+TG+VTR PL+  N DF VINP+++G++N++ Y GVGDP 
Sbjct: 477 HALEHMELVHSCVEKVRINLRTGVVTRTPLAAGNFDFPVINPAFLGRRNRYGYFGVGDPA 536

Query: 494 PKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSY 527
           PKI GV KLD  +    DC VA R FGPGC+ GEPFFV  + E +      EDDGY+V Y
Sbjct: 537 PKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFVADDVEGNG----NEDDGYLVCY 596

BLAST of CmaCh04G006050 vs. ExPASy Swiss-Prot
Match: Q5MBR6 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 5.5e-135
Identity = 258/522 (49.43%), Postives = 323/522 (61.88%), Query Frame = 0

Query: 73  SLPARFFNAFDDLINNFIN-PPVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLASSL- 132
           SLP    N  D +IN FI+ P   P+VDPR VL+ NFAPVDELPPT C VI+GS+ S L 
Sbjct: 55  SLPMAICNTVDKVINRFIDLPEQRPTVDPRRVLSGNFAPVDELPPTSCHVIRGSIPSCLA 114

Query: 133 NGAYIRNGPNPQY-------------------LPPSA----------------------- 192
            G YIRNGPNPQ+                   L PSA                       
Sbjct: 115 GGVYIRNGPNPQHRLPQRTHHLFDGDGMLHSLLIPSASSTLLSEPVLCSRYVHTYKYLLE 174

Query: 193 ---------------------ARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALG 252
                                AR  V   RVL GQ N   G GLANTS+  F D LYAL 
Sbjct: 175 RETGGPVLPNFFAGFHGVAGLARAVVMIARVLAGQINLNKGFGLANTSITLFADCLYALC 234

Query: 253 ESDLPYSIRLAP-NGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPFLTF 312
           ESDLPYS+ + P NGE+ TLGR DF G+L   MTAHPK DP T E FAFRY    PF+T+
Sbjct: 235 ESDLPYSMHINPANGEVTTLGRCDFGGDLSFRMTAHPKKDPVTMELFAFRYNVFQPFITY 294

Query: 313 FRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVGTDP 372
           F FD+ G+K +DVPI S+ +PS +HDFAIT++YA+F E Q+  NPM M+M G S VG D 
Sbjct: 295 FWFDRAGSKVADVPILSLQKPSVMHDFAITERYAIFPESQLIVNPMDMVMRGSSLVGLDR 354

Query: 373 STVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDA--VVLLAPNILSVEHTLER 432
           + V R+G++PRYA DES M WF+VP FN++H  N W+E D   +VL+APNILS+EH L  
Sbjct: 355 TMVPRIGVLPRYAKDESDMRWFEVPRFNMLHTTNGWEEADGEEIVLVAPNILSIEHMLGN 414

Query: 433 MDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKISG 492
           M+L+ A ++ VRI+L TG V+   LS  +L+FGVI+  Y+G+KN++ Y GV  P+PKI G
Sbjct: 415 MELMRARVDMVRINLCTGDVSCTALSPESLEFGVIHQGYVGRKNRYGYFGVSGPLPKIKG 474

Query: 493 VVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDEN 527
           + KLD       DC V  R FG GC+ GEPFFVP   +        ED GYVV Y H+E+
Sbjct: 475 IRKLDFDLVGSGDCTVGRRDFGLGCFAGEPFFVPDNIDGYG----NEDSGYVVCYTHEED 534

BLAST of CmaCh04G006050 vs. ExPASy Swiss-Prot
Match: Q84K96 (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=82528 GN=ZCD PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.3e-119
Identity = 212/370 (57.30%), Postives = 273/370 (73.78%), Query Frame = 0

Query: 162 QYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAP-NGEIETLGRQDFDGNL-FMSM 221
           Q +P  GIGLANTS+ F   RL+AL E DLPY +RL+P +G+I T+GR   + N+   S 
Sbjct: 2   QVDPTKGIGLANTSLQFSNGRLHALCEYDLPYVVRLSPEDGDISTVGR--IENNVSTKST 61

Query: 222 TAHPKFDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQS-DVPIFSMNRPSFLHDFAITKK 281
           TAHPK DP TGETF+F YGP+ P++T+ R+D +G K   DVPIFS   PSF+HDFAIT+ 
Sbjct: 62  TAHPKTDPVTGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEH 121

Query: 282 YAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHA 341
           YAVF +IQI   P   I+ GR  +G D   V R+GL+PRYA  +S+M WFDVPGFN++H 
Sbjct: 122 YAVFPDIQIVMKP-AEIVRGRRMIGPDLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHV 181

Query: 342 INAWDED--DAVVLLAPNILSVEHTLERMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDF 401
           +NAW+E+  + VV++APN+  +E+ ++R DL+H  +E  RI+LK+G V+R  LS  NLDF
Sbjct: 182 VNAWEEEGGEVVVIVAPNVSPIENAIDRFDLLHVSVEMARIELKSGSVSRTLLSAENLDF 241

Query: 402 GVINPSYIGKKNKFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFF 461
           GVI+  Y G+K+++ Y GVGDPMPKI GVVK+D     R +C+VA R FG GC+GGEPFF
Sbjct: 242 GVIHRGYSGRKSRYAYLGVGDPMPKIRGVVKVDFELAGRGECVVARREFGVGCFGGEPFF 301

Query: 462 VPRERESSDKTALEEDDGYVVSYVHDENFGESKFIVMDAKSPKLEIIAVVKLPRRVPYGF 521
           VP    SS K+  EEDDGYVVSY+HDE  GES F+VMDA+SP+LEI+A V LPRRVPYGF
Sbjct: 302 VP---ASSKKSGGEEDDGYVVSYLHDEGKGESSFVVMDARSPELEILAEVVLPRRVPYGF 361

Query: 522 HGLFVKESDL 527
           HGLFV E++L
Sbjct: 362 HGLFVTEAEL 365

BLAST of CmaCh04G006050 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.3e-83
Identity = 179/499 (35.87%), Postives = 264/499 (52.91%), Query Frame = 0

Query: 93  PVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLASSLNGAYIRNGPNPQYLP------- 152
           P+  + DP   +A NFAPV+E P      + G L  S+ G Y+RNG NP + P       
Sbjct: 112 PLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFF 171

Query: 153 ---------------------------------------PSA----------ARGAVAAG 212
                                                  P A          AR  +   
Sbjct: 172 DGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYA 231

Query: 213 RVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLF 272
           R   G  +PA+G G+AN  + +F  RL A+ E DLPY +++ PNG+++T+GR DFDG L 
Sbjct: 232 RAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLE 291

Query: 273 MSMTAHPKFDPDTGETFAFRYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAI 332
            +M AHPK DP++GE FA  Y  +  P+L +FRF  +G K  DV I  +++P+ +HDFAI
Sbjct: 292 STMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAI 351

Query: 333 TKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNL 392
           T+ + V  + Q+ F    MI  G SPV  D + V+R G++ +YA D S ++W D P    
Sbjct: 352 TENFVVVPDQQVVFKLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 411

Query: 393 IHAINAWD--EDDAVVLLAPNILSVEHTLERMD-LVHALMEEVRIDLKTGIVTRRPLSTR 452
            H  NAW+  E D VV++   +   +      D  + +++ E+R++LKTG  TRRP+ + 
Sbjct: 412 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 471

Query: 453 -----NLDFGVINPSYIGKKNKFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGP 512
                NL+ G++N + +G+K KF Y  + +P PK+SG  K+D++  E     V   ++G 
Sbjct: 472 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGD 531

Query: 513 GCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDENFGESKFIVMDAKSPKLEIIAVVK 527
             YGGEP F+P E         EED+GY++ +VHDE   +S+  +++A S  LE+ A VK
Sbjct: 532 NRYGGEPLFLPGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVK 591

BLAST of CmaCh04G006050 vs. ExPASy TrEMBL
Match: A0A6J1ISA3 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111479509 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.8e-301
Identity = 528/586 (90.10%), Postives = 528/586 (90.10%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLASSLNGAYIRNGPNPQYLP----------------------------------- 180
           VIQGSLASSLNGAYIRNGPNPQYLP                                   
Sbjct: 121 VIQGSLASSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGQAVLCSRYVKTYKY 180

Query: 181 ----------------------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
                                  SAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 ILERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480
           RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS
Sbjct: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 530
           GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 540

BLAST of CmaCh04G006050 vs. ExPASy TrEMBL
Match: A0A6J1FTM6 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448011 PE=3 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.0e-293
Identity = 515/586 (87.88%), Postives = 521/586 (88.91%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDALSSPFLTGGNLLLSPPIS SRPPFSMAITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLASSLNGAYIRNGPNPQYLP----------------------------------- 180
           VIQGSL SSLNGAYIRNGPNPQYLP                                   
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 ----------------------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
                                  SAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGD L
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDSL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYA+DESKM+WFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYADDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480
           RMDLVHAL+EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKN+FVYAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 530
           GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSD+TALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

BLAST of CmaCh04G006050 vs. ExPASy TrEMBL
Match: A0A346DA05 (Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 3.0e-261
Identity = 460/594 (77.44%), Postives = 491/594 (82.66%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKK----TNADSPSP--- 60
           MDA+SSPFL+GGNLLLS PIS SR   S AI SV  EETP+TVKK     +AD PSP   
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  -RRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120
            R  PPPA+AKG ST R+EPS+PA  FNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLASSLNGAYIRNGPNPQYLP--------------------------- 180
           ELPPTECEVIQGSL SSLNGAYIRNGPNPQYLP                           
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 ------------------------------PSAARGAVAAGRVLTGQYNPANGIGLANTS 240
                                          SAARGAVAAGRVLTGQYNPANGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240

Query: 241 VAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAF 300
           VAFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE FAF
Sbjct: 241 VAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMI 360
           RYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM MI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 MEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNI 420
           +EGRSPVG DPS +SR+GLIPRYANDESKM+WFDVPG NL+HAINAWDEDDAV+L+APNI
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNI 420

Query: 421 LSVEHTLERMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGV 480
           LSVEHT+ERMDLVHAL+E++RIDLKTGI+TRRPLSTRNLDFGVINP Y+GKKN+FVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGY 530
           GDPMPKISGVVKLDVSQEERRDCIVA+R+FGP CYGGEPFFVPRERE+SD+TA EEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAAEEDDGY 540

BLAST of CmaCh04G006050 vs. ExPASy TrEMBL
Match: A0A346DA10 (Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE=2 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.9e-256
Identity = 448/594 (75.42%), Postives = 485/594 (81.65%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKK----TNADSPSP--- 60
           MDA+SSPFL+GGNLLLSP IS SR   S AI SV  EETP+TVKK     +AD PSP   
Sbjct: 1   MDAISSPFLSGGNLLLSPSISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  -RRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120
            R  PPPA+AKG ST R+EPS+PA  FNAFDDLINNFINPP +PS DPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLASSLNGAYIRNGPNPQYLP--------------------------- 180
           ELPPT+CEVIQGSL SSLNGAYIRNGPNPQYLP                           
Sbjct: 121 ELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 ------------------------------PSAARGAVAAGRVLTGQYNPANGIGLANTS 240
                                          SAARGAV AGR+ TGQ+NP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240

Query: 241 VAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAF 300
           +AFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE FAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMI 360
           RYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM MI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 MEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNI 420
           +EGRSPVG DPS +SR+GLIPRYANDESKM+WFDVPG NL+HAINAWDEDDAV+L+APN+
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420

Query: 421 LSVEHTLERMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGV 480
           LSVEHT+ERMDLVHAL+E++RIDLKTGI+TRRPLSTRNLDFGVINP Y+GKKN+FVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGY 530
           GDPMPKISGVVKLDVSQEERRDCIVA+R+FGP CYGGEPFFVPRERE+SD+ A EEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDEMAAEEDDGY 540

BLAST of CmaCh04G006050 vs. ExPASy TrEMBL
Match: A0A346DA08 (Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.1e-255
Identity = 450/594 (75.76%), Postives = 485/594 (81.65%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKK----TNADSPSP--- 60
           MDA+SSPFL+GGNLLLSPP S  R   S AI SV TEETP+TVKK     +AD PSP   
Sbjct: 1   MDAISSPFLSGGNLLLSPPTSKYRTAVSTAICSVLTEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  -RRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120
            R   PPA+AKG ST R+EPS+PA  FNAFDDLINNFINPP +PS DPRYVLADNFAPVD
Sbjct: 61  QRATHPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLASSLNGAYIRNGPNPQYLP--------------------------- 180
           ELPPTECEVIQGSL SSLNGAYIRNGPNPQYLP                           
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 ------------------------------PSAARGAVAAGRVLTGQYNPANGIGLANTS 240
                                          SAARGAV AGR+ TGQ+NPANGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPANGIGLANTS 240

Query: 241 VAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAF 300
           +AFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE FAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMI 360
           RYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM MI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 MEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNI 420
           +EGRSPVG DPS +SR+GLIPRYANDESKM+WFDVPG NL+HAINAWDE+DAV+L+APNI
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEEDAVILVAPNI 420

Query: 421 LSVEHTLERMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGV 480
           LSVEHT+ERMDLVHAL+E++RIDLKTGI+TRRPLSTRNLDFGVINP Y+GKKN+FVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPVYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGY 530
           GDPMPKISGVVKLDVSQEERRDCIVA+R+FGP  YGGEPFFVPRERESSD+TA EEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNYYGGEPFFVPRERESSDETAAEEDDGY 540

BLAST of CmaCh04G006050 vs. NCBI nr
Match: XP_022979981.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.6e-301
Identity = 528/586 (90.10%), Postives = 528/586 (90.10%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLASSLNGAYIRNGPNPQYLP----------------------------------- 180
           VIQGSLASSLNGAYIRNGPNPQYLP                                   
Sbjct: 121 VIQGSLASSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGQAVLCSRYVKTYKY 180

Query: 181 ----------------------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
                                  SAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 ILERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480
           RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS
Sbjct: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 530
           GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 540

BLAST of CmaCh04G006050 vs. NCBI nr
Match: KAG6600436.1 (putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.3e-294
Identity = 516/586 (88.05%), Postives = 522/586 (89.08%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDALSSPFLTGG LLLSPPIS SRPPFSMAITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGKLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLASSLNGAYIRNGPNPQYLP----------------------------------- 180
           VIQGSL SSLNGAYIRNGPNPQYLP                                   
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 ----------------------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
                                  SAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPK DPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAIT+KYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITQKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKM+WFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480
           RMDLVHAL+EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKN+FVYAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 530
           GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSD+TALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

BLAST of CmaCh04G006050 vs. NCBI nr
Match: XP_022943214.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1018.5 bits (2632), Expect = 2.1e-293
Identity = 515/586 (87.88%), Postives = 521/586 (88.91%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDALSSPFLTGGNLLLSPPIS SRPPFSMAITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGNLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLASSLNGAYIRNGPNPQYLP----------------------------------- 180
           VIQGSL SSLNGAYIRNGPNPQYLP                                   
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 ----------------------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
                                  SAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGD L
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDSL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYA+DESKM+WFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYADDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480
           RMDLVHAL+EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKN+FVYAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 530
           GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSD+TALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

BLAST of CmaCh04G006050 vs. NCBI nr
Match: XP_023531023.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1017.7 bits (2630), Expect = 3.6e-293
Identity = 512/586 (87.37%), Postives = 522/586 (89.08%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDA+ SPFLTGGNLLLSPPIS +RPPFS+AITSVFTEETPKTVKKTNADSPSPRRPPPPA
Sbjct: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLASSLNGAYIRNGPNPQYLP----------------------------------- 180
           VIQGSL SSLNGAYIRNGPNPQYLP                                   
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 ----------------------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
                                  SAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPK DPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKM+WFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480
           RMDLVHAL+EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKN+F+YAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 530
           GVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSD+TALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

BLAST of CmaCh04G006050 vs. NCBI nr
Match: KAG7031088.1 (putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 941.4 bits (2432), Expect = 3.3e-270
Identity = 484/586 (82.59%), Postives = 490/586 (83.62%), Query Frame = 0

Query: 1   MDALSSPFLTGGNLLLSPPISISRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPA 60
           MDALSSPFLTGG LLLSPPIS SRPPFSMAITSVFTEETPKTVK+TNADSPSPRRPPPPA
Sbjct: 1   MDALSSPFLTGGKLLLSPPISTSRPPFSMAITSVFTEETPKTVKRTNADSPSPRRPPPPA 60

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120
           MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE
Sbjct: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECE 120

Query: 121 VIQGSLASSLNGAYIRNGPNPQYLP----------------------------------- 180
           VIQGSL SSLNGAYIRNGPNPQYLP                                   
Sbjct: 121 VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKY 180

Query: 181 ----------------------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240
                                  SAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL
Sbjct: 181 TLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRL 240

Query: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPF 300
           YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPK DPDTGETFAFRYGPLPPF
Sbjct: 241 YALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETFAFRYGPLPPF 300

Query: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVG 360
           LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAIT+KYAVFTEIQIGFNPMLMIMEGRSPVG
Sbjct: 301 LTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITQKYAVFTEIQIGFNPMLMIMEGRSPVG 360

Query: 361 TDPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420
           TDPSTVSRVGLIPRYANDESKM+WFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE
Sbjct: 361 TDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNILSVEHTLE 420

Query: 421 RMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKIS 480
           RMDLVHAL+EE                                KN+FVYAGVGDPMPKIS
Sbjct: 421 RMDLVHALIEE--------------------------------KNRFVYAGVGDPMPKIS 480

Query: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDE 530
           GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSD+TALEEDDGYVVSYVHDE
Sbjct: 481 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGYVVSYVHDE 540

BLAST of CmaCh04G006050 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 659.1 bits (1699), Expect = 3.1e-189
Identity = 339/577 (58.75%), Postives = 407/577 (70.54%), Query Frame = 0

Query: 23  SRPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPP--------AMAKGPSTTRLEPSL 82
           S  P  + I S   EE       TN    + RR  P          +   P   R E +L
Sbjct: 25  SSSPTLLRINSAVVEERSPI---TNPSDNNDRRNKPKTLHNRTNHTLVSSPPKLRPEMTL 84

Query: 83  PARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPV-DELPPTECEVIQGSLASSLNGA 142
               F   +D+IN FI+PP  PSVDP++VL+DNFAPV DELPPT+CE+I G+L  SLNGA
Sbjct: 85  ATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSLNGA 144

Query: 143 YIRNGPNPQYLP------------------------------------------------ 202
           YIRNGPNPQ+LP                                                
Sbjct: 145 YIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEKQTGAPVMPN 204

Query: 203 ---------PSAARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIR 262
                     S ARGA+ A RVLTGQYNP NGIGLANTS+AFF +RL+ALGESDLPY++R
Sbjct: 205 VFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALGESDLPYAVR 264

Query: 263 LAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPFLTFFRFDKNGAKQ 322
           L  +G+IET+GR DFDG L MSMTAHPK DP TGETFAFRYGP+PPFLT+FRFD  G KQ
Sbjct: 265 LTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYFRFDSAGKKQ 324

Query: 323 SDVPIFSMNRPSFLHDFAITKKYAVFTEIQIG--FNPMLMIMEGRSPVGTDPSTVSRVGL 382
            DVPIFSM  PSFLHDFAITK++A+F EIQ+G   N + +++EG SPVGTD     R+G+
Sbjct: 325 RDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGKTPRLGV 384

Query: 383 IPRYANDESKMEWFDVPGFNLIHAINAWDEDD--AVVLLAPNILSVEHTLERMDLVHALM 442
           IP+YA DES+M+WF+VPGFN+IHAINAWDEDD  +VVL+APNI+S+EHTLERMDLVHAL+
Sbjct: 385 IPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMSIEHTLERMDLVHALV 444

Query: 443 EEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDPMPKISGVVKLDVSQ 502
           E+V+IDL TGIV R P+S RNLDF VINP+++G+ +++VYA +GDPMPKISGVVKLDVS+
Sbjct: 445 EKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKISGVVKLDVSK 504

Query: 503 EERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDENFGESKFIV 530
            +R DC VA R++G GCYGGEPFFV R+  + +    EEDDGYVV+YVHDE  GESKF+V
Sbjct: 505 GDRDDCTVARRMYGSGCYGGEPFFVARDPGNPE---AEEDDGYVVTYVHDEVTGESKFLV 564

BLAST of CmaCh04G006050 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 312.0 bits (798), Expect = 9.2e-85
Identity = 179/499 (35.87%), Postives = 264/499 (52.91%), Query Frame = 0

Query: 93  PVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLASSLNGAYIRNGPNPQYLP------- 152
           P+  + DP   +A NFAPV+E P      + G L  S+ G Y+RNG NP + P       
Sbjct: 112 PLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFF 171

Query: 153 ---------------------------------------PSA----------ARGAVAAG 212
                                                  P A          AR  +   
Sbjct: 172 DGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYA 231

Query: 213 RVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLF 272
           R   G  +PA+G G+AN  + +F  RL A+ E DLPY +++ PNG+++T+GR DFDG L 
Sbjct: 232 RAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLE 291

Query: 273 MSMTAHPKFDPDTGETFAFRYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAI 332
            +M AHPK DP++GE FA  Y  +  P+L +FRF  +G K  DV I  +++P+ +HDFAI
Sbjct: 292 STMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAI 351

Query: 333 TKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNL 392
           T+ + V  + Q+ F    MI  G SPV  D + V+R G++ +YA D S ++W D P    
Sbjct: 352 TENFVVVPDQQVVFKLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 411

Query: 393 IHAINAWD--EDDAVVLLAPNILSVEHTLERMD-LVHALMEEVRIDLKTGIVTRRPLSTR 452
            H  NAW+  E D VV++   +   +      D  + +++ E+R++LKTG  TRRP+ + 
Sbjct: 412 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 471

Query: 453 -----NLDFGVINPSYIGKKNKFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGP 512
                NL+ G++N + +G+K KF Y  + +P PK+SG  K+D++  E     V   ++G 
Sbjct: 472 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGD 531

Query: 513 GCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDENFGESKFIVMDAKSPKLEIIAVVK 527
             YGGEP F+P E         EED+GY++ +VHDE   +S+  +++A S  LE+ A VK
Sbjct: 532 NRYGGEPLFLPGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVK 591

BLAST of CmaCh04G006050 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 308.9 bits (790), Expect = 7.8e-84
Identity = 196/577 (33.97%), Postives = 289/577 (50.09%), Query Frame = 0

Query: 17  SPPISIS---RPPFSMAITSVFTEETPKTVKKTNADSPSPRRPPPPAMAKGPSTTRLEPS 76
           SP  S+S     P    +++    +TP  +   N  SP+P  P        P    L+ +
Sbjct: 27  SPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKDTSRWNP----LQRA 86

Query: 77  LPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLASSLNGA 136
             A    A   L+    + P+  +VDPR+ ++ N+APV E        + G +   ++G 
Sbjct: 87  ASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGV 146

Query: 137 YIRNGPNPQYLPPSA--------------------------------------------- 196
           Y+RNG NP + P S                                              
Sbjct: 147 YLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPK 206

Query: 197 -----------ARGAVAAGRVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRL 256
                      AR  +   R L G  N  NG G+AN  + +F DRL A+ E DLPY +R+
Sbjct: 207 AIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRV 266

Query: 257 APNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPL-PPFLTFFRFDKNGAKQ 316
             NG++ET+GR DFDG L  +M AHPK DP T E FA  Y  +  P+L +F+F   G K 
Sbjct: 267 TDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKS 326

Query: 317 SDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIP 376
            DV I  +  P+ +HDFAIT+ + V  + Q+ F    M + G+SPV  D   +SR G++P
Sbjct: 327 PDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKYDGEKISRFGILP 386

Query: 377 RYANDESKMEWFDVPGFNLIHAINAWD--EDDAVVLLAPNILSVEHTLERMD-LVHALME 436
           R A D S+M W + P     H  NAW+  E D VV++   +   +      D  +++++ 
Sbjct: 387 RNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLS 446

Query: 437 EVRIDLKTGIVTRRPL----STRNLDFGVINPSYIGKKNKFVYAGVGDPMPKISGVVKLD 496
           E+R++LKTG  TRR +       NL+ G++N + +G+K ++ Y  + +P PK+SG  K+D
Sbjct: 447 EIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVD 506

Query: 497 VSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDENFGESK 527
           +S  E     V +  +G   YGGEPFF+PR  ES       EDDGY++S+VHDE   ES+
Sbjct: 507 LSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-----EDDGYIMSFVHDEESWESE 566

BLAST of CmaCh04G006050 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 303.9 bits (777), Expect = 2.5e-82
Identity = 181/536 (33.77%), Postives = 273/536 (50.93%), Query Frame = 0

Query: 61  MAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPV-DELPPTEC 120
           ++ G S   + P     F +   DL+   +   +  +  P + L+ NFAP+ DE PP + 
Sbjct: 5   LSDGSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKD 64

Query: 121 EVIQGSLASSLNGAYIRNGPNPQYLPPSA-----ARGAVAAGRVLTGQ------------ 180
             + G L   LNG ++R GPNP++   +        G +   R+  G+            
Sbjct: 65  LPVHGFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSR 124

Query: 181 --------------------------------------YNPANGIGLANTSVAFFGDRLY 240
                                                  +   G G ANT++ +   +L 
Sbjct: 125 LKQEEFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHGKLL 184

Query: 241 ALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAFRYGPLPPFL 300
           AL E+D PY I++  +G+++TLG  D+D  L  S TAHPK DP TGE F F Y   PP+L
Sbjct: 185 ALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYL 244

Query: 301 TFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMIMEGRSPVGT 360
           T+    K+G     VPI +++ P  +HDFAIT+ YA+F ++ + F P  M+ E +     
Sbjct: 245 TYRVISKDGIMHDPVPI-TISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSF 304

Query: 361 DPSTVSRVGLIPRYANDESKMEWFDVPGFNLIHAINAWDEDDAVVLLA-----PNILSVE 420
           DP+  +R G++PRYA DE  + WF++P   + H  NAW+E+D VVL+      P++  V 
Sbjct: 305 DPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDLDMVS 364

Query: 421 HTL-ERMDLVHALMEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNKFVYAGVGDP 480
             + E+++     + E+R ++KTG  +++ LS   +DF  IN  Y GKK ++VY  + D 
Sbjct: 365 GKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGTILDS 424

Query: 481 MPKISGVVKLDVSQEE---RRDCIVASRI-----FGPGCYGGEPFFVPRERESSDKTALE 527
           + K++G++K D+  E    +R   V   I      G G YG E  +VPRE         E
Sbjct: 425 IAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRE-------TAE 484

BLAST of CmaCh04G006050 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 288.1 bits (736), Expect = 1.4e-77
Identity = 173/501 (34.53%), Postives = 259/501 (51.70%), Query Frame = 0

Query: 93  PVSPSVDPRYVLADNFAPVDELPPTECEVIQGSLASSLNGAYIRNGPNPQYLPPSA---- 152
           P   + DP   +A NF PV E P      + G++   + G Y+RNG NP + P S     
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLF 231

Query: 153 -----------ARGAVAAG----------------------------------------- 212
                        G+V+                                           
Sbjct: 232 DGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNT 291

Query: 213 RVLTGQYNPANGIGLANTSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLF 272
           R L G  +P  G+G+AN  + +F   L A+ E DLPY +++   G++ET GR DFDG L 
Sbjct: 292 RGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLK 351

Query: 273 MSMTAHPKFDPDTGETFAFRYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAI 332
            +M AHPK DP+T E FA  Y  +  P+L +FRF  +G K  DV I  +++P+ +HDFAI
Sbjct: 352 STMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEI-PLDQPTMIHDFAI 411

Query: 333 TKKYAVFTEIQIGFNPMLMIMEGRSPVGTDPSTVSRVGLIPRYANDESKMEWFDVPGFNL 392
           T+ + V  + Q+ F    MI  G SPV  D    SR G++ + A D S ++W +VP    
Sbjct: 412 TENFVVIPDQQVVFRLPEMI-RGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFC 471

Query: 393 IHAINAWDE---DDAVV----LLAPNILSVEHTLERMDLVHALMEEVRIDLKTGIVTRRP 452
            H  N+W+E   D+ VV    +  P+ +  EH     + + +++ E+R++LKTG  TRRP
Sbjct: 472 FHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHD----ETLQSVLSEIRLNLKTGESTRRP 531

Query: 453 LSTR--NLDFGVINPSYIGKKNKFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFG 512
           + +   NL+ G++N + +G+K ++ Y  + +P PK+SG  K+D+S  E     +   I+G
Sbjct: 532 VISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGE-----IRKYIYG 591

Query: 513 PGCYGGEPFFVPRERESSDKTALEEDDGYVVSYVHDENFGESKFIVMDAKSPKLEIIAVV 528
            G YGGEP F+P    S D    EED GY++ +VHDE   +S+  +++A + KLE  A V
Sbjct: 592 EGKYGGEPLFLP----SGDG---EEDGGYIMVFVHDEEKVKSELQLINAVNMKLE--ATV 651

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O496754.3e-18858.75Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thalian... [more]
Q6YVJ01.2e-16152.139-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q5MBR65.5e-13549.439-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q84K963.3e-11957.30Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=8... [more]
Q9LRR71.3e-8335.879-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1ISA31.8e-30190.10probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita maxima OX... [more]
A0A6J1FTM61.0e-29387.88probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata ... [more]
A0A346DA053.0e-26177.44Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A346DA102.9e-25675.42Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE... [more]
A0A346DA081.1e-25575.76Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2... [more]
Match NameE-valueIdentityDescription
XP_022979981.13.6e-30190.10probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita maxima][more]
KAG6600436.13.3e-29488.05putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita ar... [more]
XP_022943214.12.1e-29387.88probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata][more]
XP_023531023.13.6e-29387.37probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita pepo subsp.... [more]
KAG7031088.13.3e-27082.59putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita ar... [more]
Match NameE-valueIdentityDescription
AT4G19170.13.1e-18958.75nine-cis-epoxycarotenoid dioxygenase 4 [more]
AT3G14440.19.2e-8535.87nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.17.8e-8433.97nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT3G63520.12.5e-8233.77carotenoid cleavage dioxygenase 1 [more]
AT1G78390.11.4e-7734.53nine-cis-epoxycarotenoid dioxygenase 9 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 164..521
e-value: 5.9E-79
score: 266.4
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 147..527
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 30..146
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..71
NoneNo IPR availablePANTHERPTHR10543:SF46CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATEDcoord: 147..527
NoneNo IPR availablePANTHERPTHR10543:SF46CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATEDcoord: 30..146

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G006050.1CmaCh04G006050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen