CmaCh04G005310 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G005310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSerine hydroxymethyltransferase
LocationCma_Chr04: 2705656 .. 2710889 (-)
RNA-Seq ExpressionCmaCh04G005310
SyntenyCmaCh04G005310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCCAGGTTCGTCCTTCGACTTCCACAGCCGAAATAAGATTCATCCCGTCATCGTCCAGTTCTTCTTCCCTTCCCTTCTGTATCCCTTTACCTCCATTTTTAGAGAGATTTCTCTTAGTTTTCAGGAATTTTGTTGTTTCCGGGCTTTCCATGGACGTTTCATTGAGTTCAATGGAACCAACATTCGAGCTTCTCTGTTTTACCGACCTCAATCGCCTCTGCTGCTGTTTTTCTTGTTGTCGCTGTTGTTGCTGATCAGGAAATTCTGTTGGGGTTTATTTCGTTCAATTTTTCTATCTCCGATTGTGGTTGTTTACAGGAGGTGTAACTTTTGGGGGATTGGATTTTGTTTGCTAGGGTTAAGACTCTGATTCTTCGCTTTGTTCGTAGTTAATGGATTTGTCTAAGTCCCATTCCAACCTTTCTCTTGGATTCTCATCGCATGCTTCCCCGCCTCACCGTGCTCAGATTGCCGATGATTCCGTCTTGCTGCATCTCGATTCTTCTCGACGGGAATCCTCGTATTCGTCCACCCCAGTCCCTCTTCAGTTGATGGAACAGCAGACGGAGAGTCACAGAAATGATGGTGGAGATGCGGAGAGAGATGACGATGAATACGACGACAGGGAGGTAGAAGAGTTTCGGATTTTGGGTCATCCTATGTGTTTGAAGCGTCGAAGAGATGGCGAATCTTCGACATCCTCCAGTTCTGCGAAAAGAATTTCGTGTGAATTTGATATAGAGACACGAAGGAGTGCTGTACGGTCATGGGGTGATCAGACCCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAAGCAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCTGAAAATTATGTGTGTCGGGCTGTAATGGAAGCATTGGGAAGCCATTTGACTAATAAGTACTCGGAAGGTATGCCCGGTGCGAGGTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTATGTCGTGAGCGCGCATTGGCGGCATTTGGTCTTAGTTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACATCTGCAAACTTTGCTGTCTACACTGGTCTGTTATTGCCGGGCGATCGGATCATGGGGTTGGATACTCCTTCTGGTGGTAATACAAGCCATGGGTACTGTACCCCAAATGGAAGAAAAGTTTCTGGGGCCTCAATTTTCTTTGAGAGTTTGCCATATAAGGTGAATCCACAAACTGGTTATATCGATTATGATAAGCTTGAGGAGAGGGCACTTGACTTTCGCCCAAAAATACTTATCTGTGGTGGGAGCTCGTATCCTCGTGAACTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAATGTGGAGCAGCTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCCAAGGTGTGATTTTTTCTCCATCTAAATACGACCCTTGAATATCTGTGTATATATCTTTTGGTTTTTCTTATCACATGTACTAGTTACTATCCTGTCTTCATTCTCTTGGGTCTCTATTTAGTTGGTTGTTAATATAATTAAGATATTTGAATTGTTTTACCTGATATTCAGACATGTCTTCAAAGGTGGCTGTGTGGGGACTTCATTGTGGTTTTTGGTATTTAGGATATGTATGGCTTTGGCTATGAGACTTAGTGATACGAAAATGTAATTGATTGAAGTATTTTGGGACAAGACGTGTTGGTACATTGGCCAATCGTGAATGCTTTGAATCATTAAGCTTATGACTGTGGCATTGTTTTTCTTTTCTACTTTTATTAAAGATAGTTTTTGTTTCTACGTGCTTCTATTCTTCATTTTCATTCAATAGTGTATTTATGCATTTTTCAGTTTTTTTTAGGTCTTCATGTTATAACAATGCCTTGAATATATATATTTTTTAATAACCCGATATGGGGTCTCCTATGCCCGCCCCAAGATCTGGACCAGGAGCCTTTTCAATTTGTTGAACCTTTTTTTGTTACTTTTTTTATGGTAGAGTGCATGTTTTGACACCCGTTTCATAGTCACTAAAGCTTGCACTTTCGTTACTCGGTGAATTGTTATTTCCTTTTTTTAAAACAAAATAGTATGTTGCTCTTTGACATCTGTGTTTTTTTTTTCTACTTATATTGTTTGATCCATCAGTACTTTTTATGTGTAATCTTGTAGTTTTAGATGGAAAATTATCCCTACTCTTCCGTTCATATTTTGATTTTGGTTTGAATTTTCTGACAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAAAGTCTTCGAGGTCCGAGGGGAGGTATAATTTTTTATAGGAAGGGTAGTAAGCCGAGGAAGAGAGCAATGCTATTGTATCAGGGAAATGAAACTGACCAATATGATTTTGAGGAGAAGATAAACTTTGCCGTTTTTCCCTCTTTACAAGGTGGACCTCACAATAACCATATTGCTGCTCTTGCTGTGGCCCTAAAGCAAGTGGCTACTCCCGAGTACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTGCTGCTTTACTTCGAAAGAAGTACAAGCTTGTAACTGGTGGCACGGATAACCACATGATACTCTGGGATCTAAGGTCCTTGGGATTGACAGGTGCATAATTTTGACTCGAATGTTGCAAATTGATGTGAACATGATATATTTAAGCTGCACAACTAGCTTTTTGTTTGTCTAGGGGGAAGTGGCACACAAAAAACGTTGCTCAAGCACAGCGAGGGTTGATTAAGTTATGCACTGTGGGTTATGATTAGGGGAAATGCCATATTATATTATTATTGCATTACATGATTTACATAATTTGGAATAAGTATATCTCTTATATCAACTGAATTGCCAAACAAAATAGGATTTTTTTTTTTTTTTTATATGGTTAAATTAGCTTAAAAACTACTTGCTTCGGCACAAATATGATTTTTATTTAGCAATATCTGGTACTTTTTTTCTTTTTCATTTCATAGATTATGTCTGACTTTTCATTTCATAGATTATGTCTGACTTTTCATTTCATAGATTATGTCTGACTTTTCATTTCATAGATTATGTCTGACTTTTCATTTCATAGATTATGCCTGTGCATTTAATTACTCTAATAAGAAAGCTGAATACATTCTTTAATAAGGTGTTAAAAGTGATGAAACTGTGGACAAGGCATGCAACTGTTAGTCATGGCAAGTCTTAGCATAGCCTTCAAAACTCACCAAAACTATGGAAAAGTACAGAAAGGGTGATGAGAAATCAACCCAAAAAGGCCATTAGGAATTGGATAAGTTTCTCCAATTGGCAGCGACTAATGATGATTGCAGTTACTAAAAACAAAAAAAAAAAAAGGGTTTTTTTAAGGTACTCCAAGAAGCCTTTTATTCAATGAAGATGAACTCTAAACTTCTGAAATAATTTATGATTTCATTCTAGTCGAACTTCCACGAGTAAAGATTCTTTGCTTTGTACTTTAAGGGATGCCTCCTTGGCAAACAATCCAGAATTTGCAGAACAGAAAAGGGTAGGCATTAAGCAAAATTAAACTTATCTAGCAGAGTTCCTCAAAGATTTCTGTAGCAGTATAGGAATAGACTGTTTTTGGCTTTTGAACCATCTTTATCTTGAGCAGCACAGCTATAAGAGATGAGTCTGGGGATTTCTTCTTTGTGTAATATCAGACATATTGATGCCCGTTATCACTAAGTTTCACAGAACTTCTTGTCTTCTTAGGGCTTCCTTTCTGCCAAATGACTCCAAAAGTCTCTTGTTAAGAGTGGTGTAAAATCTATTACAACGCACAACTTGTTTTTTGTTCATATTTGTATGCGTCTATTTCTTCTGAGTCCTAATTTTGATGGTATCTGTGGTGGCTTAGTCTATTATTCTACATTTATATTGTGTAACTTATTGATGGCATGGTAATTGTAGGTAAAAACTATGAGAATGTTTGTGAGATCTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAACGGAATTATCATACCCGGAGGAGTACGGGTTGGTGAGTAATGCCCTCTTATATTTCATATTTTGGGCTCCTTTTATTCGAGTTTTTTATGTATGCCTTTTTATTGTATTCATTTTCTCAATCAACACTTAGTTTCATACCGAAAGAAATATCTTGGGGTATCCTATGTTTGGAGAGCTTAGTATGCGTTATAAATTGGCTCACTGTTACCCCTGCAGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAGACAATTGCTGATTTTCTTCATACAGCCGCTCAGATTGCAATTTCAGTACAGCGAGAGTATGGGAAGCTACCAAAGACTTATCTCAAGGGCCTTCTAAGTAATAATGAAGTGGTTGAGCTCCGAAACCGTGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCAGGCTTTGAAATTTGAATGGTAGTTGCAATTGCTAGTGAAATGGACAACCATCCGTAGTCCTCAAAATTCGTAATTTCCGCTCATCATGCCTTCCATATATACAATATATGAAGCTTCTCCCTCTTGGACTTGCTGTCTTCAAAATTGTTTGGTTGCTATTCAGTTGCCAGTAGTTCATATCATCTTATGGTTTGGAAGGTGTTGTAATTATCAGAAGAGCATTTGGTAATACCGAGCTTTTCTTCTGTTCGGCAGAAGGCATACAATGGATAGGAGGTTATGTGCAGTTTTGTGGTGGTATCATTTTTTGGAGCAAATCAACTGAAGATATTGTTTGGTACGGGAGAATAATTAAATTCTAGTACTCTAAATTATCAGAGTTCTAGAAGAATGTTTTGGATCTGATTGTTTATGGAAACGGCTCAGGAATCATTTTGCCAGTTTGATATGTGATGATGTGGTAGGGAAGTATTCACAATGCTGATCAAGTTCCTTTGATCAGTTTCCATTGTGTAAGTTCTTGCTACATTTCCTTCATCATAAATTATGTTATATAAGTGTCTATTCATCAACCTAGTTCATTTCTCTGAAGGTCTAGTCGATTTATTTGTCCACGTTTGTTGATAGAAAATTCAGCTATGGTTCTTGCACTTATTTTATTCTTCTCCCATGTTTGATAGTAATATGATAATTGTTTCAACACTGCAGAAGCTGATGCAATTGTGATAAATAAATTGATAAGATGATATATTTGCA

mRNA sequence

GTCCAGGTTCGTCCTTCGACTTCCACAGCCGAAATAAGATTCATCCCGTCATCGTCCAGTTCTTCTTCCCTTCCCTTCTGTATCCCTTTACCTCCATTTTTAGAGAGATTTCTCTTAGTTTTCAGGAATTTTGTTGTTTCCGGGCTTTCCATGGACGTTTCATTGAGTTCAATGGAACCAACATTCGAGCTTCTCTGTTTTACCGACCTCAATCGCCTCTGCTGCTGTTTTTCTTGTTGTCGCTGTTGTTGCTGATCAGGAAATTCTGTTGGGGTTTATTTCGTTCAATTTTTCTATCTCCGATTGTGGTTGTTTACAGGAGGTGTAACTTTTGGGGGATTGGATTTTGTTTGCTAGGGTTAAGACTCTGATTCTTCGCTTTGTTCGTAGTTAATGGATTTGTCTAAGTCCCATTCCAACCTTTCTCTTGGATTCTCATCGCATGCTTCCCCGCCTCACCGTGCTCAGATTGCCGATGATTCCGTCTTGCTGCATCTCGATTCTTCTCGACGGGAATCCTCGTATTCGTCCACCCCAGTCCCTCTTCAGTTGATGGAACAGCAGACGGAGAGTCACAGAAATGATGGTGGAGATGCGGAGAGAGATGACGATGAATACGACGACAGGGAGGTAGAAGAGTTTCGGATTTTGGGTCATCCTATGTGTTTGAAGCGTCGAAGAGATGGCGAATCTTCGACATCCTCCAGTTCTGCGAAAAGAATTTCGTGTGAATTTGATATAGAGACACGAAGGAGTGCTGTACGGTCATGGGGTGATCAGACCCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAAGCAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCTGAAAATTATGTGTGTCGGGCTGTAATGGAAGCATTGGGAAGCCATTTGACTAATAAGTACTCGGAAGGTATGCCCGGTGCGAGGTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTATGTCGTGAGCGCGCATTGGCGGCATTTGGTCTTAGTTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACATCTGCAAACTTTGCTGTCTACACTGGTCTGTTATTGCCGGGCGATCGGATCATGGGGTTGGATACTCCTTCTGGTGGTAATACAAGCCATGGGTACTGTACCCCAAATGGAAGAAAAGTTTCTGGGGCCTCAATTTTCTTTGAGAGTTTGCCATATAAGGTGAATCCACAAACTGGTTATATCGATTATGATAAGCTTGAGGAGAGGGCACTTGACTTTCGCCCAAAAATACTTATCTGTGGTGGGAGCTCGTATCCTCGTGAACTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAATGTGGAGCAGCTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCCAAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAAAGTCTTCGAGGTCCGAGGGGAGGTATAATTTTTTATAGGAAGGGTAGTAAGCCGAGGAAGAGAGCAATGCTATTGTATCAGGGAAATGAAACTGACCAATATGATTTTGAGGAGAAGATAAACTTTGCCGTTTTTCCCTCTTTACAAGGTGGACCTCACAATAACCATATTGCTGCTCTTGCTGTGGCCCTAAAGCAAGTGGCTACTCCCGAGTACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTGCTGCTTTACTTCGAAAGAAGTACAAGCTTGTAACTGGTGGCACGGATAACCACATGATACTCTGGGATCTAAGGTCCTTGGGATTGACAGGTAAAAACTATGAGAATGTTTGTGAGATCTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAACGGAATTATCATACCCGGAGGAGTACGGGTTGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAGACAATTGCTGATTTTCTTCATACAGCCGCTCAGATTGCAATTTCAGTACAGCGAGAGTATGGGAAGCTACCAAAGACTTATCTCAAGGGCCTTCTAAGTAATAATGAAGTGGTTGAGCTCCGAAACCGTGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCAGGCTTTGAAATTTGAATGGTAGTTGCAATTGCTAGTGAAATGGACAACCATCCGTAGTCCTCAAAATTCGTAATTTCCGCTCATCATGCCTTCCATATATACAATATATGAAGCTTCTCCCTCTTGGACTTGCTGTCTTCAAAATTGTTTGGTTGCTATTCAGTTGCCAGTAGTTCATATCATCTTATGGTTTGGAAGGTGTTGTAATTATCAGAAGAGCATTTGGTAATACCGAGCTTTTCTTCTGTTCGGCAGAAGGCATACAATGGATAGGAGGTTATGTGCAGTTTTGTGGTGGTATCATTTTTTGGAGCAAATCAACTGAAGATATTGTTTGGTACGGGAGAATAATTAAATTCTAGTACTCTAAATTATCAGAGTTCTAGAAGAATGTTTTGGATCTGATTGTTTATGGAAACGGCTCAGGAATCATTTTGCCAGTTTGATATGTGATGATGTGGTAGGGAAGTATTCACAATGCTGATCAAGTTCCTTTGATCAGTTTCCATTGTGTAAGTTCTTGCTACATTTCCTTCATCATAAATTATGTTATATAAGTGTCTATTCATCAACCTAGTTCATTTCTCTGAAGGTCTAGTCGATTTATTTGTCCACGTTTGTTGATAGAAAATTCAGCTATGGTTCTTGCACTTATTTTATTCTTCTCCCATGTTTGATAGTAATATGATAATTGTTTCAACACTGCAGAAGCTGATGCAATTGTGATAAATAAATTGATAAGATGATATATTTGCA

Coding sequence (CDS)

ATGGATTTGTCTAAGTCCCATTCCAACCTTTCTCTTGGATTCTCATCGCATGCTTCCCCGCCTCACCGTGCTCAGATTGCCGATGATTCCGTCTTGCTGCATCTCGATTCTTCTCGACGGGAATCCTCGTATTCGTCCACCCCAGTCCCTCTTCAGTTGATGGAACAGCAGACGGAGAGTCACAGAAATGATGGTGGAGATGCGGAGAGAGATGACGATGAATACGACGACAGGGAGGTAGAAGAGTTTCGGATTTTGGGTCATCCTATGTGTTTGAAGCGTCGAAGAGATGGCGAATCTTCGACATCCTCCAGTTCTGCGAAAAGAATTTCGTGTGAATTTGATATAGAGACACGAAGGAGTGCTGTACGGTCATGGGGTGATCAGACCCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAAGCAGAGGCAATTCAAGGGCATTGAATTGATTGCTTCTGAAAATTATGTGTGTCGGGCTGTAATGGAAGCATTGGGAAGCCATTTGACTAATAAGTACTCGGAAGGTATGCCCGGTGCGAGGTATTACTGTGGAAATCAATATATAGATGAGATTGAAACCCTATGTCGTGAGCGCGCATTGGCGGCATTTGGTCTTAGTTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACATCTGCAAACTTTGCTGTCTACACTGGTCTGTTATTGCCGGGCGATCGGATCATGGGGTTGGATACTCCTTCTGGTGGTAATACAAGCCATGGGTACTGTACCCCAAATGGAAGAAAAGTTTCTGGGGCCTCAATTTTCTTTGAGAGTTTGCCATATAAGGTGAATCCACAAACTGGTTATATCGATTATGATAAGCTTGAGGAGAGGGCACTTGACTTTCGCCCAAAAATACTTATCTGTGGTGGGAGCTCGTATCCTCGTGAACTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAATGTGGAGCAGCTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCCAAGGAATGTGTCAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAAAGTCTTCGAGGTCCGAGGGGAGGTATAATTTTTTATAGGAAGGGTAGTAAGCCGAGGAAGAGAGCAATGCTATTGTATCAGGGAAATGAAACTGACCAATATGATTTTGAGGAGAAGATAAACTTTGCCGTTTTTCCCTCTTTACAAGGTGGACCTCACAATAACCATATTGCTGCTCTTGCTGTGGCCCTAAAGCAAGTGGCTACTCCCGAGTACAAGGCATATATGCAGCAGGTGAAAAAAAATGCTCAAGCCTTGGCTGCTGCTTTACTTCGAAAGAAGTACAAGCTTGTAACTGGTGGCACGGATAACCACATGATACTCTGGGATCTAAGGTCCTTGGGATTGACAGGTAAAAACTATGAGAATGTTTGTGAGATCTGCCACATTACTCTCAACAAAATTACAATATTTGGTGATAACGGAATTATCATACCCGGAGGAGTACGGGTTGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAGACAATTGCTGATTTTCTTCATACAGCCGCTCAGATTGCAATTTCAGTACAGCGAGAGTATGGGAAGCTACCAAAGACTTATCTCAAGGGCCTTCTAAGTAATAATGAAGTGGTTGAGCTCCGAAACCGTGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCAGGCTTTGAAATTTGA

Protein sequence

MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTESHRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI
Homology
BLAST of CmaCh04G005310 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 1.1e-240
Identity = 416/597 (69.68%), Postives = 482/597 (80.74%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFS-SHAS----PPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLME 60
           MDLS+S +N  LGF  SHAS    P  RA IADDS+ L +D S R    + +P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  QQTE------SHRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGE-SSTSSSSAK 120
           Q+ E        + DGG         D +E E FRILGH MCLKR+RD     T S   K
Sbjct: 61  QKAEKTTTVDEPKKDGGGGG------DQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPK 120

Query: 121 RISC-EFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVM 180
           R S  + D+E+RR+AVR+WGDQ + +ADP++  +MEKEKQRQ +GIELIASEN+VCRAVM
Sbjct: 121 RSSIGDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVM 180

Query: 181 EALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTS 240
           EALGSHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL SD WGVNVQPYSCTS
Sbjct: 181 EALGSHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTS 240

Query: 241 ANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYI 300
           ANFAVYTGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYI
Sbjct: 241 ANFAVYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYI 300

Query: 301 DYDKLEERALDFRPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKEC 360
           DYDKLE++ALD+RPKILICGGSSYPR+ D+AR RQIADKCGA LMCDMA ISGLVA KEC
Sbjct: 301 DYDKLEDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKEC 360

Query: 361 VSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAV 420
            +PF +CD+VTSTTHK LRGPRGGIIFYR+G K RK+       + +  YD EEKINFAV
Sbjct: 361 SNPFDHCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAV 420

Query: 421 FPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDN 480
           FPSLQGGPHNNHIAALA+ALKQVATPEYKAY+QQ+KKNAQALAAALLR+K +LVTGGTDN
Sbjct: 421 FPSLQGGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDN 480

Query: 481 HMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLES 540
           H++LWDL  +GLTGK YE VCE+CHITLNK  IFGDNG I PGGVR+GTPAMT+RGC+ES
Sbjct: 481 HLLLWDLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIES 540

Query: 541 DFETIADFLHTAAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMP 585
           DFET+ADFL  AAQI  ++QRE+GK  K ++K L +N ++ ELRNRVE+F+ Q+ MP
Sbjct: 541 DFETMADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of CmaCh04G005310 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.5e-237
Identity = 408/595 (68.57%), Postives = 486/595 (81.68%), Query Frame = 0

Query: 6   SHSNLSLGF-SSHASP---PHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTESH 65
           + S+LSLGF SSHA P   P R  IADDS+ L +DSS R SS    PVPLQL+EQ+ +  
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  RNDGGDAERDD------DEYDDREVEEFRILGHPMCLKRRRDGESST--SSSSAKRISCE 125
            +     E DD      D+ D RE E+F +LGHPM LKR R G S +  SSS  KR   +
Sbjct: 65  GSCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVD 124

Query: 126 FDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSH 185
             IE+RR+ VR+WG+Q++  ADP +   MEKEKQRQF+GIELIASEN+VCRAVMEALGSH
Sbjct: 125 SGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSH 184

Query: 186 LTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVY 245
           LTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFAV+
Sbjct: 185 LTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVF 244

Query: 246 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 305
           TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKLE
Sbjct: 245 TGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLE 304

Query: 306 ERALDFRPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGY 365
           E+ALD+RPKILICGGSSYPR+ ++ RFR IADKCGA LM DMAQISGLVAAKE  +PF Y
Sbjct: 305 EKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDY 364

Query: 366 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 425
           CD+VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQG
Sbjct: 365 CDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQG 424

Query: 426 GPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWD 485
           GPHNNHIAALA+ALKQ A+PEYK YM+QVKKNA+ALA+AL+ +K KL+TGGTDNH++LWD
Sbjct: 425 GPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWD 484

Query: 486 LRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIA 545
           L  LGLTGK YE VCE+CHIT+NK+ IF +NG+I PGGVR+G+PAMTSRGCLE +FET+A
Sbjct: 485 LTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMA 544

Query: 546 DFLHTAAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
           DFL+ AAQIA + QRE+GKL K  LK +    E+ +LRN+VE+F+ QFAMP F++
Sbjct: 545 DFLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of CmaCh04G005310 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.4e-174
Identity = 295/464 (63.58%), Postives = 355/464 (76.51%), Query Frame = 0

Query: 123 VRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWG+ +L   DP +  ++EKEK+RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY GN++IDEIE LCR RAL AF      WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+ DYARFR IADK GA L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGK 482
           LAVALKQ  TP +K Y +QVK NA AL   L+ K Y++VT GT+NH++LWDLR LGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAAQI 542
             E +C++C ITLNK  +FGD+  + PGGVR+G PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGF 587
            + +Q+ YGKL K + KGL++N ++ +L+  VE FSA + MPGF
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of CmaCh04G005310 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 1.1e-173
Identity = 290/466 (62.23%), Postives = 353/466 (75.75%), Query Frame = 0

Query: 123 VRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWG+  L   DP ++ ++EKEK RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY G ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPRE DYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGK 482
           LAVALKQV  P +K Y +QVK NA  LA+ L+ K Y LVT GTDNH+ILWDLR LGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAAQI 542
             E VCE+C+ITLN+  +FGD   + PGGVR+GTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
            + +Q +YGK+ K + KGL++N E+ E++  VE F+  F MPGF I
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of CmaCh04G005310 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 5.5e-152
Identity = 265/459 (57.73%), Postives = 332/459 (72.33%), Query Frame = 0

Query: 127 GDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGAR 186
           G+  L+  D  +F +M +EK RQFKG+ELIASEN+  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 187 YYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGL 246
           YY G + +DE+ETLC++RAL AF L    WGVNVQPYS + ANFAVYT LL P DRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 247 DTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILIC 306
           D PSGG+ +HGY T + +K+S +SIFFES+PY++    G IDY +LEE AL F+PK++I 
Sbjct: 126 DLPSGGHLTHGYQT-DKKKISASSIFFESMPYQIGAD-GLIDYQRLEENALLFKPKLIIS 185

Query: 307 GGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLR 366
           G S+YPRE DY R R IADK GA LMCDMA  SGLVAA+   SPF YCDVVTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 367 GPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVA 426
           GPR GIIF+R+G +      +   G E ++YD E KINFAVFPSLQGGPH N IA +AVA
Sbjct: 246 GPRSGIIFFRRGKR------VDGNGKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVA 305

Query: 427 LKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGKNYEN 486
           LK+  + E+K Y  QVKKNA A+  AL+ K YKLVT GTDNH+ILWDLR   LTG  +E 
Sbjct: 306 LKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEK 365

Query: 487 VCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAAQIAISV 546
             +I +IT+NK  + GD   I PGG+R+G+ A+TSRG  E+DFE IADFL     I++ +
Sbjct: 366 AADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEI 425

Query: 547 QREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPG 586
           Q   GK    ++  +  + E+++LR  VE FS++F +PG
Sbjct: 426 QGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

BLAST of CmaCh04G005310 vs. ExPASy TrEMBL
Match: A0A6J1IXT5 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3 SV=1)

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
           QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 588

BLAST of CmaCh04G005310 vs. ExPASy TrEMBL
Match: A0A6J1FV71 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE=3 SV=1)

HSP 1 Score: 1187.2 bits (3070), Expect = 0.0e+00
Identity = 585/588 (99.49%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HRNDGGDAERDDDEYDDREVEEFRILGH MCLKRRRDGESSTSS SAKRISCEFDIETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
           QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 588

BLAST of CmaCh04G005310 vs. ExPASy TrEMBL
Match: A0A6J1D7A8 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 548/587 (93.36%), Postives = 567/587 (96.59%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS SHSNLSLGFSSHASPPHRAQIADDSVLLHLDS+RRE  YSS PVPLQLME QTES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HR++ G+AER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SS S KR+SCEFD++ RR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGL SDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQ+ATPEYKAYMQQVKKNAQALA+ALLRKKY+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKNYE VCEIC ITLNKITI+GDNGIIIPGGVR+GTPAMTSRGCLESDFETI DFLHTAA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQRE+GKLPK YLKGL +NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of CmaCh04G005310 vs. ExPASy TrEMBL
Match: A0A6J1E9K3 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE=3 SV=1)

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 525/587 (89.44%), Postives = 550/587 (93.70%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS SHSNLSLG SSHASPPHR QIADDS+LLHL+SSR+E ++SS P+PLQLME  TES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HR++ GDAER+DD YDDR+VEEFRILGH MCLKRRRD ESS SSSS+KR SCE D+ETRR
Sbjct: 61  HRDEVGDAEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSLSSSSSKRNSCERDLETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLR  DPNLFGIMEKEKQRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRATDPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVAT EYKAYMQQVKKNAQALA+ALLRK YKLVTGGTDNHM+LWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKN+E VCE+CHITLNKITIFGDNGII PGGVR+GTPAMTSRGCLESDFE I +FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAI VQRE GKLPK +L+GL SNNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAIVVQRENGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 587

BLAST of CmaCh04G005310 vs. ExPASy TrEMBL
Match: A0A6J1JAX6 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 7.2e-304
Identity = 517/587 (88.07%), Postives = 546/587 (93.02%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS S+SNLSLG SSHASPPHR QIADDS+LLHL+SSR+E ++SS P+PLQLME  TES
Sbjct: 1   MDLSNSNSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HR++ G AER+    DDR+VEEF ILGH MCLKRRRD ESS SSSSAKR SCE D+ETRR
Sbjct: 61  HRDEVGGAERE----DDRDVEEFHILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLR ADPNLFGIMEKEKQRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYRRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGS+PR  AMLLYQGNE++QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSRPRTIAMLLYQGNESNQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVAT EYKAYMQQVKKNAQALA+ALLRK YKLVTGGTDNHM+LWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKN+E VCE+CHITLNKITIFGDNGII PGGVR+GTPAMTSRGCLESDFE I +FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 588
           QIA+ VQRE+GKLPK +L+GL S+NEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAVVVQREHGKLPKPFLRGLQSSNEVVELGYRVASFSAKFAMPGLE 583

BLAST of CmaCh04G005310 vs. NCBI nr
Match: XP_022979958.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
           QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 588

BLAST of CmaCh04G005310 vs. NCBI nr
Match: XP_023547556.1 (serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 585/588 (99.49%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HRNDGGD ERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSS SAKRISCEFDIETRR
Sbjct: 61  HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
           QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 588

BLAST of CmaCh04G005310 vs. NCBI nr
Match: XP_022941930.1 (serine hydroxymethyltransferase 7-like [Cucurbita moschata] >KAG6600365.1 Serine hydroxymethyltransferase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031023.1 Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1187.2 bits (3070), Expect = 0.0e+00
Identity = 585/588 (99.49%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HRNDGGDAERDDDEYDDREVEEFRILGH MCLKRRRDGESSTSS SAKRISCEFDIETRR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
           QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 588

BLAST of CmaCh04G005310 vs. NCBI nr
Match: XP_022149204.1 (serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149207.1 serine hydroxymethyltransferase 7-like [Momordica charantia])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 548/587 (93.36%), Postives = 567/587 (96.59%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS SHSNLSLGFSSHASPPHRAQIADDSVLLHLDS+RRE  YSS PVPLQLME QTES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HR++ G+AER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SS S KR+SCEFD++ RR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGL SDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQ+ATPEYKAYMQQVKKNAQALA+ALLRKKY+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKNYE VCEIC ITLNKITI+GDNGIIIPGGVR+GTPAMTSRGCLESDFETI DFLHTAA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAISVQRE+GKLPK YLKGL +NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of CmaCh04G005310 vs. NCBI nr
Match: KAG6576983.1 (Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015003.1 Serine hydroxymethyltransferase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 526/587 (89.61%), Postives = 551/587 (93.87%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60
           MDLS SHSNLSLG SSHASPPHR QIADDS+LLHL+SSR+E ++SS P+PLQLME  TES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120
           HR++ GD ER+DD YDDR+VEEFRILGH MCLKRRRD ESS SSSSAKR SCE D+ETRR
Sbjct: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLR ADPNLFGIMEKEKQRQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYCGNQYIDEIETLCRERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480
           AALAVALKQVAT EYKAYMQQVKKNAQALA+ALLRK YKLVTGGTDNHM+LWDLRSLGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540
           GKN+E VCE+CHITLNKITIFGDNGII PGGVR+GTPAMTSRGCLESDFE I +FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 588
           QIAI VQRE+GKLPK +L+GL SNNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 587

BLAST of CmaCh04G005310 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 833.9 bits (2153), Expect = 7.7e-242
Identity = 416/597 (69.68%), Postives = 482/597 (80.74%), Query Frame = 0

Query: 1   MDLSKSHSNLSLGFS-SHAS----PPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLME 60
           MDLS+S +N  LGF  SHAS    P  RA IADDS+ L +D S R    + +P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  QQTE------SHRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGE-SSTSSSSAK 120
           Q+ E        + DGG         D +E E FRILGH MCLKR+RD     T S   K
Sbjct: 61  QKAEKTTTVDEPKKDGGGGG------DQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPK 120

Query: 121 RISC-EFDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVM 180
           R S  + D+E+RR+AVR+WGDQ + +ADP++  +MEKEKQRQ +GIELIASEN+VCRAVM
Sbjct: 121 RSSIGDSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVM 180

Query: 181 EALGSHLTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTS 240
           EALGSHLTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL SD WGVNVQPYSCTS
Sbjct: 181 EALGSHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTS 240

Query: 241 ANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYI 300
           ANFAVYTGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYI
Sbjct: 241 ANFAVYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYI 300

Query: 301 DYDKLEERALDFRPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKEC 360
           DYDKLE++ALD+RPKILICGGSSYPR+ D+AR RQIADKCGA LMCDMA ISGLVA KEC
Sbjct: 301 DYDKLEDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKEC 360

Query: 361 VSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAV 420
            +PF +CD+VTSTTHK LRGPRGGIIFYR+G K RK+       + +  YD EEKINFAV
Sbjct: 361 SNPFDHCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAV 420

Query: 421 FPSLQGGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDN 480
           FPSLQGGPHNNHIAALA+ALKQVATPEYKAY+QQ+KKNAQALAAALLR+K +LVTGGTDN
Sbjct: 421 FPSLQGGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDN 480

Query: 481 HMILWDLRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLES 540
           H++LWDL  +GLTGK YE VCE+CHITLNK  IFGDNG I PGGVR+GTPAMT+RGC+ES
Sbjct: 481 HLLLWDLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIES 540

Query: 541 DFETIADFLHTAAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMP 585
           DFET+ADFL  AAQI  ++QRE+GK  K ++K L +N ++ ELRNRVE+F+ Q+ MP
Sbjct: 541 DFETMADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of CmaCh04G005310 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 822.8 bits (2124), Expect = 1.8e-238
Identity = 408/595 (68.57%), Postives = 486/595 (81.68%), Query Frame = 0

Query: 6   SHSNLSLGF-SSHASP---PHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTESH 65
           + S+LSLGF SSHA P   P R  IADDS+ L +DSS R SS    PVPLQL+EQ+ +  
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  RNDGGDAERDD------DEYDDREVEEFRILGHPMCLKRRRDGESST--SSSSAKRISCE 125
            +     E DD      D+ D RE E+F +LGHPM LKR R G S +  SSS  KR   +
Sbjct: 65  GSCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVD 124

Query: 126 FDIETRRSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSH 185
             IE+RR+ VR+WG+Q++  ADP +   MEKEKQRQF+GIELIASEN+VCRAVMEALGSH
Sbjct: 125 SGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSH 184

Query: 186 LTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVY 245
           LTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFAV+
Sbjct: 185 LTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVF 244

Query: 246 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 305
           TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKLE
Sbjct: 245 TGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLE 304

Query: 306 ERALDFRPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGY 365
           E+ALD+RPKILICGGSSYPR+ ++ RFR IADKCGA LM DMAQISGLVAAKE  +PF Y
Sbjct: 305 EKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDY 364

Query: 366 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 425
           CD+VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQG
Sbjct: 365 CDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQG 424

Query: 426 GPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWD 485
           GPHNNHIAALA+ALKQ A+PEYK YM+QVKKNA+ALA+AL+ +K KL+TGGTDNH++LWD
Sbjct: 425 GPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWD 484

Query: 486 LRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIA 545
           L  LGLTGK YE VCE+CHIT+NK+ IF +NG+I PGGVR+G+PAMTSRGCLE +FET+A
Sbjct: 485 LTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMA 544

Query: 546 DFLHTAAQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
           DFL+ AAQIA + QRE+GKL K  LK +    E+ +LRN+VE+F+ QFAMP F++
Sbjct: 545 DFLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of CmaCh04G005310 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 614.4 bits (1583), Expect = 9.6e-176
Identity = 295/464 (63.58%), Postives = 355/464 (76.51%), Query Frame = 0

Query: 123 VRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWG+ +L   DP +  ++EKEK+RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY GN++IDEIE LCR RAL AF      WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+ DYARFR IADK GA L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGK 482
           LAVALKQ  TP +K Y +QVK NA AL   L+ K Y++VT GT+NH++LWDLR LGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAAQI 542
             E +C++C ITLNK  +FGD+  + PGGVR+G PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGF 587
            + +Q+ YGKL K + KGL++N ++ +L+  VE FSA + MPGF
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of CmaCh04G005310 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 611.3 bits (1575), Expect = 8.2e-175
Identity = 290/466 (62.23%), Postives = 353/466 (75.75%), Query Frame = 0

Query: 123 VRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWG+  L   DP ++ ++EKEK RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYY G ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPRE DYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLTGK 482
           LAVALKQV  P +K Y +QVK NA  LA+ L+ K Y LVT GTDNH+ILWDLR LGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAAQI 542
             E VCE+C+ITLN+  +FGD   + PGGVR+GTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFEI 589
            + +Q +YGK+ K + KGL++N E+ E++  VE F+  F MPGF I
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of CmaCh04G005310 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 486.1 bits (1250), Expect = 4.0e-137
Identity = 252/479 (52.61%), Postives = 327/479 (68.27%), Query Frame = 0

Query: 117 ETRRSAVRSWGDQ---TLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSH 176
           E  RS V +W  Q    L   DP +  I+E EK RQ+KG+ELI SEN+   +VM+A+GS 
Sbjct: 39  EKERSRV-TWPKQLNAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSV 98

Query: 177 LTNKYSEGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVY 236
           +TNKYSEG PGARYY GN+YID  ETLC++RAL AF L  + WGVNVQP S + ANF VY
Sbjct: 99  MTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVY 158

Query: 237 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 296
           T LL P +RIM LD P GG+ SHGY T + +K+S  SIFFE++PY+++  TGYIDYD++E
Sbjct: 159 TALLKPHERIMALDLPHGGHLSHGYQT-DTKKISAVSIFFETMPYRLDESTGYIDYDQME 218

Query: 297 ERALDFRPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGY 356
           + A  FRPK+++ G S+Y R  DYAR R++ +K  A ++ DMA ISGLVAA    SPF Y
Sbjct: 219 KSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDY 278

Query: 357 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 416
            DVVT+TTHKSLRGPRG +IF+RKG K   +     QG E   YDFE+KIN AVFP LQG
Sbjct: 279 ADVVTTTTHKSLRGPRGAMIFFRKGVKEINK-----QGKEV-LYDFEDKINQAVFPGLQG 338

Query: 417 GPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWD 476
           GPHN+ I  LAVALKQ  T EYKAY +QV  N+   A  L+ + Y+LV+GGTDNH++L +
Sbjct: 339 GPHNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVN 398

Query: 477 LRSLGLTGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIA 536
           L+  G+ G   E V E  HI  NK T+ GD   ++PGG+R+GTPA+TSRG +E DF  +A
Sbjct: 399 LKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVA 458

Query: 537 DFLHTAAQIAISVQRE-YGKLPKTYLKGLLSN----NEVVELRNRVESFSAQFAMPGFE 588
           ++   A  IA+ V+ E  G   K ++  + S+    +E+ +LR+ VE F+ QF   GFE
Sbjct: 459 EYFDKAVTIALKVKSEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84WV01.1e-24069.68Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
Q9LM592.5e-23768.57Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
O232541.4e-17463.58Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM41.1e-17362.23Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q54Z265.5e-15257.73Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IXT50.0e+00100.00Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3... [more]
A0A6J1FV710.0e+0099.49Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE... [more]
A0A6J1D7A80.0e+0093.36Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 P... [more]
A0A6J1E9K30.0e+0089.44Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE... [more]
A0A6J1JAX67.2e-30488.07Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3... [more]
Match NameE-valueIdentityDescription
XP_022979958.10.0e+00100.00serine hydroxymethyltransferase 7-like [Cucurbita maxima][more]
XP_023547556.10.0e+0099.49serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022941930.10.0e+0099.49serine hydroxymethyltransferase 7-like [Cucurbita moschata] >KAG6600365.1 Serine... [more]
XP_022149204.10.0e+0093.36serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 ser... [more]
KAG6576983.10.0e+0089.61Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT1G36370.17.7e-24269.68serine hydroxymethyltransferase 7 [more]
AT1G22020.11.8e-23868.57serine hydroxymethyltransferase 6 [more]
AT4G13930.19.6e-17663.58serine hydroxymethyltransferase 4 [more]
AT4G13890.18.2e-17562.23Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G37930.14.0e-13752.61serine transhydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 132..535
e-value: 4.0E-170
score: 565.7
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 160..440
e-value: 2.2E-193
score: 644.6
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 135..584
e-value: 2.2E-193
score: 644.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..59
NoneNo IPR availablePANTHERPTHR11680:SF45SERINE HYDROXYMETHYLTRANSFERASEcoord: 74..588
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 74..588
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 130..585
score: 39.764168
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 135..577
e-value: 0.0
score: 554.89
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 355..371
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 122..586

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G005310.1CmaCh04G005310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity