CmaCh04G001830 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh04G001830 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 2.8e-266
Identity = 464/730 (63.56%), Postives = 567/730 (77.67%), Query Frame = 0

Query: 1   MGSSVIFKLVFLG-FFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVV 60
           M SS+IFKL F   FF   LAS LDSD+DG+ +YIVY+G K +D  S   HH  +LE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 61  GSTFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGF 120
           GSTF+P+++LH+YKRSFNGFAV+LTEEEA+KI + +GVVSVF N    LHTTRSWDF+GF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 121 TTNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIG 180
              VPR +QVES+IVVGVLD+GIWPESPSF D G+ P P KWKG C+ SNNFRCN+KIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 181 ARAYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIA 240
           AR+Y       P D   PRD++GHGTHTAST AGGLVSQA+LYGL LGTARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 241 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGIL 300
            YK+CW+DGC D DILAA+DDAIADGVDIISLSVGG+ P++YF D+IAIG+FH+++ GIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 301 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKH 360
           TSNSAGN GP++FT  + SPW LSVAAS++DRK V+ VQ+GN   FQG +INTFD Q  +
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ--Y 360

Query: 361 YPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSP-STFASLSSAVGV 420
           YPL+     PN   GF  S+SR+C+  SV+ NL+KGKI+VC++   P   F SL  A GV
Sbjct: 361 YPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGV 420

Query: 421 VMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSF 480
           +M  +  +D A SYPLPSS L          YI S   P ATIFKS  + + +AP+VVSF
Sbjct: 421 LM-TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 480

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHAT 540
           SSRGPN  T D++KPD++ PGVEILAAW  +APV  GI+  R TL+NIISGTSMSCPH T
Sbjct: 481 SSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGIR--RNTLFNIISGTSMSCPHIT 540

Query: 541 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDA 600
             A YVKT++PTWSPAAIKSALMTTA P+N   NPQAEFAYG+GH++PLKA+ PGL+YDA
Sbjct: 541 GIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDA 600

Query: 601 NETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFT 660
           NE+DYV FLCGQGY+T  VRR+TGD S CT+ N+GRVWDLNYPSF LS +PS++ NQ+F 
Sbjct: 601 NESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFN 660

Query: 661 RTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSAS 720
           RTLT+V  +ASTY + ++ AP+GLTI+V+P VLSFNG+G ++SFTLT+ G+I   +VSAS
Sbjct: 661 RTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSAS 720

Query: 721 VVWSDGSHNV 729
           +VWSDG H V
Sbjct: 721 LVWSDGVHYV 721

BLAST of CmaCh04G001830 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 2.0e-187
Identity = 347/722 (48.06%), Postives = 470/722 (65.10%), Query Frame = 0

Query: 25  SDNDGRK---VYIVYLGNKPDDMASTPSHHMRLLEEVVGSTFSPDALLHSYKRSFNGFAV 84
           S ND R+   VYIVY+G  P+   S PSHH+ +L+++VG+  +   L+ SYKRSFNGFA 
Sbjct: 22  SANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAA 81

Query: 85  RLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVGVLDSG 144
            L++ E+QK++    VVSVFP+    L TTRSWDF+GF     R +  ESD++VGV+DSG
Sbjct: 82  NLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSG 141

Query: 145 IWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDFESPRDSD 204
           IWPES SF D G+GP P KWKG+C+    F CN K+IGAR Y   N F     +S RD +
Sbjct: 142 IWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY---NKFA----DSARDEE 201

Query: 205 GHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDA 264
           GHGTHTAST AG  V  AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFDDA
Sbjct: 202 GHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDA 261

Query: 265 IADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWS 324
           IADGVD+IS+S+         N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW 
Sbjct: 262 IADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWM 321

Query: 325 LSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGGFSGSSSR 384
           ++VAAS  DR+ +  V LGN     G ++NTF+L G  +P++Y     N+S   S + + 
Sbjct: 322 ITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAG 381

Query: 385 YCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPLPSSYLTR 444
           YCS   VD  LVKGKI++CD  L     A L+ A+GV++ N  + D+A   P P+S L  
Sbjct: 382 YCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGF 441

Query: 445 AAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPDLTAPGVE 504
                +K+YI S   P A I ++ E+ D  AP V SFSSRGP+    ++LKPD++APG+E
Sbjct: 442 EDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLE 501

Query: 505 ILAAWAPIAPVSSGI--KDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSA 564
           ILAA++P+A  SS +  +D R+  Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA
Sbjct: 502 ILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSA 561

Query: 565 LMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGYSTAMVRR 624
           +MTTA P+N++ NP+ EFAYG+G I+P KA +PGL+Y+    DY+  LC +G+ +  +  
Sbjct: 562 IMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTT 621

Query: 625 LTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEASTYTSKILGAP 684
            +G    C+      V DLNYP+     +  +  N  F RT+TNVG   STY + ++   
Sbjct: 622 TSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQ 681

Query: 685 EGLTITVDPPVLSFNGIGHKESFTLTIGGTISQ--SIVSASVVWSDGSHNVYVVYMGTSF 740
             L I+++P +L F  +  K+SF +TI G   +  S VS+SVVWSDGSH+V    +  S 
Sbjct: 682 PELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 729

BLAST of CmaCh04G001830 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.9e-177
Identity = 341/737 (46.27%), Postives = 465/737 (63.09%), Query Frame = 0

Query: 4   SVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTP-SHHMRLLEEVVGST 63
           S IF L+ + F      +S   D+  ++VYIVY+G  P  +   P SHH  +L++V G +
Sbjct: 11  SCIFALLVVSF------ASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGES 70

Query: 64  FSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFT-- 123
              D L+ +YKRSFNGFA RLTE E + + + D VVSVFP+   +L TT SW+FMG    
Sbjct: 71  SIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEG 130

Query: 124 TNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGA 183
               R   +ESD ++GV+DSGI+PES SFS  G+GP P KWKG C+   NF CN K+IGA
Sbjct: 131 KRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGA 190

Query: 184 RAYRSD-NNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIA 243
           R Y      FP    ES RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARIA
Sbjct: 191 RYYTPKLEGFP----ESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIA 250

Query: 244 VYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHG 303
           VYK+C      C    ILAAFDDAIAD VDII++S+G      +  D++AIGAFH+M  G
Sbjct: 251 VYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKG 310

Query: 304 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQG 363
           ILT N AGN+GP+  TI + +PW  +VAAS+++R  ++ V LGN     G ++N+FDL G
Sbjct: 311 ILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNG 370

Query: 364 KHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVG 423
           K YPL+Y   A   S     SS+ +CS   +D   VKGKI++CD+  +P    ++ +   
Sbjct: 371 KKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVAS 430

Query: 424 VVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVS 483
           +V N    +D A  +  P S L+    N V +Y+ S   P A + KS  + +  AP+V S
Sbjct: 431 IVRN--PYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVAS 490

Query: 484 FSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHA 543
           +SSRGPNP  +DILKPD+TAPG EILAA++P  P S    D+R   Y +ISGTSMSCPH 
Sbjct: 491 YSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHV 550

Query: 544 TAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQ---AEFAYGAGHIDPLKAMNPGL 603
              A Y+KTFHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH+DP+ A++PGL
Sbjct: 551 AGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGL 610

Query: 604 IYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVW-DLNYPSFALSTTPSESI 663
           +Y+AN++D++ FLCG  Y+   +R ++GD S CT   +  +  +LNYPS +   + ++  
Sbjct: 611 VYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPF 670

Query: 664 NQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGT--IS 723
              F RT+TNVG   +TY +K++G+   L + V P VLS   +  K+SFT+T+ G    +
Sbjct: 671 KVTFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKA 728

Query: 724 QSIVSASVVWSDGSHNV 729
           +++VSA ++WSDG H V
Sbjct: 731 ENLVSAQLIWSDGVHFV 728

BLAST of CmaCh04G001830 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 6.0e-176
Identity = 333/733 (45.43%), Postives = 467/733 (63.71%), Query Frame = 0

Query: 11  FLGFFGCLLASSLDS------DNDGRKVYIVYLGNKPDDMASTP-SHHMRLLEEVVGSTF 70
           F+  F  LL  SL S      D+  ++VYIVYLG+ P     TP S HM +L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 71  SPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNV 130
             + L+ SYK+SFNGFA RLTE E +++   + VVSVFP+ K  L TT SW+FMG    +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 131 --PRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGAR 190
              R   +ESD ++GV+DSGI+PES SFSD G+GP P KWKG C    NF CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 191 AYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVY 250
            Y + +    +  ++ RD  GHGTHTAS  AG  V+ ++ YGL  GTARGGVP+ARIAVY
Sbjct: 187 DYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 251 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTS 310
           K+C ++GC    +++AFDDAIADGVD+IS+S+       +  D IAIGAFH+M  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 311 NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYP 370
           N+AGN+GP   T+ + +PW  SVAAS  +R  ++ V LG+  I  G ++NT+D+ G +YP
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 371 LIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMN 430
           L+Y   A       S   +R C    +D  LVKGKI++CDS         L +   +V N
Sbjct: 367 LVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKN 426

Query: 431 NAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSR 490
               +   RS+P+  S+L+      + +Y+ S   P AT+ KS E+++  AP+V SFSSR
Sbjct: 427 PEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSR 486

Query: 491 GPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAA 550
           GP+    DILKPD+TAPGVEILAA++P +  +    D+R   Y+++SGTSM+CPH    A
Sbjct: 487 GPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVA 546

Query: 551 VYVKTFHPTWSPAAIKSALMTTAIPLNVELN--PQAEFAYGAGHIDPLKAMNPGLIYDAN 610
            YVKTFHP WSP+ I+SA+MTTA P+N   +     EFAYG+GH+DP+ A+NPGL+Y+  
Sbjct: 547 AYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELT 606

Query: 611 ETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRV-WDLNYPSFALSTTPSESINQFFT 670
           + D++NFLCG  Y++  +R ++GD S CT   S  +  +LNYP+ +   + ++  N  F 
Sbjct: 607 KADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQ 666

Query: 671 RTLTNVGSEASTYTSKILGAP-EGLTITVDPPVLSFNGIGHKESFTLTIGGTI--SQSIV 729
           RT+TNVG + STY +K++  P   L+I V P VLS   +  K+SF +T+      ++  V
Sbjct: 667 RTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPV 726

BLAST of CmaCh04G001830 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.3e-175
Identity = 335/720 (46.53%), Postives = 453/720 (62.92%), Query Frame = 0

Query: 18  LLASSLDSDNDGRKVYIVYLGNKPDDMASTP-SHHMRLLEEVVGSTFSPDALLHSYKRSF 77
           L  SS+ +  D ++VYIVY+G+       TP S HM +L+EV G +     L+ SYKRSF
Sbjct: 17  LFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSF 76

Query: 78  NGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNV--PRVNQVESDIV 137
           NGFA RLTE E +++    GVVSVFPN K  L TT SWDFMG    +   R   VESD +
Sbjct: 77  NGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTI 136

Query: 138 VGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDF 197
           +GV+DSGI PES SFSD G+GP P KWKG C    NF CN K+IGAR Y S         
Sbjct: 137 IGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS--------- 196

Query: 198 ESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADI 257
           E  RD DGHGTHTAST AG  V  AS +G+  GT RGGVP++R+A YK+C   GC    +
Sbjct: 197 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 256

Query: 258 LAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTI 317
           L+AFDDAIADGVD+I++S+G      + ND IAIGAFH+M  G+LT NSAGN GP   ++
Sbjct: 257 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 316

Query: 318 RNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGG 377
              +PW L+VAAS+ +R  V+ V LGN     G ++N ++++GK YPL+Y   A   S  
Sbjct: 317 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSA--ASSA 376

Query: 378 FSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPL 437
               S+  C  + VD++ VKGKILVC          S+  AVG++       D A  +PL
Sbjct: 377 CDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLIYRTP-KPDVAFIHPL 436

Query: 438 PSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPD 497
           P++ L       + +Y+ S + P A + K+  + +  +P++ SFSSRGPN    DILKPD
Sbjct: 437 PAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPD 496

Query: 498 LTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPA 557
           +TAPGVEILAA++P    S    D+R   Y+++SGTSMSCPH    A YVKTF+P WSP+
Sbjct: 497 ITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPS 556

Query: 558 AIKSALMTTAIPLNVELN--PQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGY 617
            I+SA+MTTA P+N         EFAYG+GH+DP+ A NPGL+Y+ +++D++ FLCG  Y
Sbjct: 557 MIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNY 616

Query: 618 STAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPS-ESINQFFTRTLTNVGSEASTY 677
           ++ +++ ++G+   C+ A      +LNYPS +   + S  +    F RTLTNVG+  STY
Sbjct: 617 TSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTY 676

Query: 678 TSKIL-GAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIV--SASVVWSDGSHNV 729
           TSK++ G    L + + P VLSF  +  K+SFT+T+ G+   S V  SA+++WSDG+HNV
Sbjct: 677 TSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNV 721

BLAST of CmaCh04G001830 vs. ExPASy TrEMBL
Match: F6HNS9 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_13s0019g02490 PE=3 SV=1)

HSP 1 Score: 1666.4 bits (4314), Expect = 0.0e+00
Identity = 864/1468 (58.86%), Postives = 1062/1468 (72.34%), Query Frame = 0

Query: 17   CLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGSTFSPDALLHSYKRSF 76
            C  +++  S +DGRK YIVY+G KP    S  + H+ +L++V GS+ +  +L+ SYKRSF
Sbjct: 21   CTHSTAAASKDDGRKEYIVYMGAKPAGDFSASAIHIDMLQQVFGSSRASISLVRSYKRSF 80

Query: 77   NGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVG 136
            NGF  +LTEEE Q+++  DGVVS+FPN KK LHTTRSWDF+GF   V R + +ESDI++G
Sbjct: 81   NGFVAKLTEEEMQQMKGMDGVVSIFPNEKKQLHTTRSWDFVGFPQQVKRTS-IESDIIIG 140

Query: 137  VLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDFES 196
            VLDSGIWPES SF D G+GP P+KW G CQ  +NF CN KIIGA+ YRS   F  EDF+S
Sbjct: 141  VLDSGIWPESDSFDDEGFGPPPSKWIGTCQGFSNFTCNNKIIGAKYYRSSGQFRQEDFQS 200

Query: 197  PRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILA 256
            PRDS+GHGTHTAST AGGLVS ASL G  LGTARGGVPSARIAVYKICWSDGC+ ADILA
Sbjct: 201  PRDSEGHGTHTASTAAGGLVSMASLMGFGLGTARGGVPSARIAVYKICWSDGCFGADILA 260

Query: 257  AFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRN 316
            AFDDAIADGVDIIS+SVGG  P  YF D IAIGAFH+MK  ILTS SAGNDGP   +I N
Sbjct: 261  AFDDAIADGVDIISISVGGKTPTNYFEDPIAIGAFHAMKKRILTSASAGNDGPVLASITN 320

Query: 317  FSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGGFS 376
            FSPWSLSVAAS+IDR   + VQLG+ N+F+G +INTF+L    YPLIY G APN + GFS
Sbjct: 321  FSPWSLSVAASTIDRDFFTKVQLGDSNVFEGVSINTFELNDM-YPLIYGGDAPNTAAGFS 380

Query: 377  GSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPLPS 436
            G+ SR+C  ++++ NLVKGKI++CD + +    A L+ AVG +M +   KD++RS+PLP+
Sbjct: 381  GNRSRFCFPSTLNPNLVKGKIVLCD-VKTNGAGAFLAGAVGALMADTLPKDSSRSFPLPA 440

Query: 437  SYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPDLT 496
            S+L+   G+ + NYI S + PTA+IFKS EV+D  AP VVSFSSRGPNP ++D+LKPD+ 
Sbjct: 441  SHLSARDGSSIANYINSTSNPTASIFKSTEVSDALAPYVVSFSSRGPNPASFDLLKPDIA 500

Query: 497  APGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAI 556
            APGV ILAAW PIAPVS    D+R  LYNIISGTSMSCPHA+ AA Y+K+F+PTWSPAAI
Sbjct: 501  APGVRILAAWPPIAPVSGVKGDNREVLYNIISGTSMSCPHASGAAAYIKSFNPTWSPAAI 560

Query: 557  KSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGYSTAM 616
            KSALMTTA P++ + NP+AEFAYGAG+IDP+KA++PGL+YDA+E DYV FLCGQGYST  
Sbjct: 561  KSALMTTATPMSAKKNPEAEFAYGAGNIDPVKAIDPGLVYDADEIDYVKFLCGQGYSTPA 620

Query: 617  VRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEASTYTSKIL 676
            +R +TGD SVC+AA +G VW+LNYPSFALS+   ESI   F RT+TNVGS  STY + ++
Sbjct: 621  LRLVTGDNSVCSAATNGTVWNLNYPSFALSSLTKESITGMFNRTVTNVGSSVSTYKATVI 680

Query: 677  GAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQ------------------------ 736
            GAPEGL I V+P +LSF  +  K SF L + G + +                        
Sbjct: 681  GAPEGLEIQVEPSILSFTSLMQKLSFVLKVEGKVERERRLEGSSIYMYACPSTTKPIAYH 740

Query: 737  -----------------------SIVSASVVWSDGSHNVYVVYMGTSFEPDYSTASKQHI 796
                                   S  S +    D     Y+VYMG     D+S AS  H 
Sbjct: 741  HLMARQKNSSLWFLLLSLICSLLSTHSTAAASEDDVRKEYIVYMGAKPAGDFS-ASVIHT 800

Query: 797  NMLQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKLAEMDGVVSMFVSEKKHLQTTR 856
            NML+QV     AS SL+ SY RSF+GF A L ++E +++  MDGVVS+F SEKK L TTR
Sbjct: 801  NMLEQVFGSDRASSSLVRSYKRSFNGFVAKLTEDEMQQMKGMDGVVSVFPSEKKQLHTTR 860

Query: 857  SWDFMGLFEGAPRTKLETDLIIGILDTGIWPESESFSDEGFDPPPAKWKGECQSSYNFTC 916
            SWDF+G      RT +E+D+IIG+LD GIWPES+SF D+GF PPP KWKG CQ   NFTC
Sbjct: 861  SWDFVGFPRQVKRTSVESDIIIGVLDGGIWPESDSFDDKGFGPPPRKWKGTCQGFSNFTC 920

Query: 917  NNKIIGARSFRSGATINCEHDNLSPRDTEGHGTLTSSTAAGNLVTGASLFGLGLGTSRGG 976
            NNKIIGA+ ++S    + E D  SPRD++GHGT T+STAAG LV  ASL G GLGT+RGG
Sbjct: 921  NNKIIGAKYYKSDRKFSPE-DLQSPRDSDGHGTHTASTAAGGLVNMASLMGFGLGTARGG 980

Query: 977  SPSARIAVYKICWSEGCFDADILAAFDHAIADGVDIISLSVAGSDLKDYFEDSIAIGAFH 1036
             PSARIAVYKICWS+GC DADILAAFD AIADGVDIIS S+     +DYF+D+ AIGAFH
Sbjct: 981  VPSARIAVYKICWSDGCDDADILAAFDDAIADGVDIISYSLGNPPSQDYFKDTAAIGAFH 1040

Query: 1037 AMKSGILTSNAGGNTGPLFGTIQNVSPWSLSVAASTTDRKFVTNVKLGNGDSFLGISVNT 1096
            AMK+GILTS + GN GP   ++ +VSPWSLSVAAST DRKF+T V+LG+   + G S+N 
Sbjct: 1041 AMKNGILTSTSAGNDGPRLVSVVSVSPWSLSVAASTIDRKFLTEVQLGDRKVYKGFSINA 1100

Query: 1097 FQLGDKLFPLIYAGDAPNTTAGFNGSSSRFCFNGSLDVNLIQGKIVLCDGIADG--EVS- 1156
            F+  + ++PLIY GDAPNT  GF G++SRFC   SL+ NL++GKIVLC G+  G  E S 
Sbjct: 1101 FE-PNGMYPLIYGGDAPNTRGGFRGNTSRFCEKNSLNPNLVKGKIVLCIGLGAGLEETSN 1160

Query: 1157 ---SGAVGMIMQDDTL--QEAALLFPLPASQLDFNAGKNVFQYMRSTSNPVASIEKSTAT 1216
               +GAVG ++ D     ++++ ++PLPAS+L    GK +  Y+ STSNP ASI KS   
Sbjct: 1161 AFLAGAVGTVIVDGLRFPKDSSYIYPLPASRLGAGDGKRIAYYISSTSNPTASILKSIEV 1220

Query: 1217 KDLLAPYVVSFSSRGPNLLTPDILKPDLAAPGVDILASWPEGSAITGFTGDNRKAPFNIV 1276
            KD LAPYV SFSSRGPN +T D+LKPDL APGV ILA+W   S I+  +GDNR A +NI+
Sbjct: 1221 KDTLAPYVPSFSSRGPNNITHDLLKPDLTAPGVHILAAWSPISPISQMSGDNRVAQYNIL 1280

Query: 1277 SGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAEAEFAYGAGHLNPV 1336
            SGTS+ACPHATGAAAY+KSFHPTWSPAAIKSALMTTA PM+ + N EAEFAYGAG+++PV
Sbjct: 1281 SGTSMACPHATGAAAYIKSFHPTWSPAAIKSALMTTATPMSARKNPEAEFAYGAGNIDPV 1340

Query: 1337 NATNPGLVYDAQYIDYIEFLCGQGYSTENLRLVTGDQINCSDVRKTAASHLNYPSFALLI 1396
             A +PGLVYDA  ID++ FLCG+GYS + LR VTGD   CS     A   LNYPSFAL  
Sbjct: 1341 RAVHPGLVYDADEIDFVNFLCGEGYSFQTLRKVTGDHSACSKATNGAVWDLNYPSFALST 1400

Query: 1397 ESQRLATCIYHRTVTNVGLSVSTYKA-VIQAPSGLKVRVYPTTLSFSSLGQKISFTVRVE 1429
             ++      +HR+VTNVG  +STYKA VI AP GLK+ V P  LSF+S+GQK+SF ++V 
Sbjct: 1401 SNKESIARTFHRSVTNVGSPMSTYKAIVIGAPKGLKINVKPNILSFTSIGQKLSFVLKVN 1460

BLAST of CmaCh04G001830 vs. ExPASy TrEMBL
Match: A0A5B6WJM8 (Cucumisin-like OS=Gossypium australe OX=47621 GN=EPI10_022228 PE=3 SV=1)

HSP 1 Score: 1645.6 bits (4260), Expect = 0.0e+00
Identity = 843/1438 (58.62%), Postives = 1033/1438 (71.84%), Query Frame = 0

Query: 9    LVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGSTF-SPDA 68
            L  + F   +L       +D R+VYIVY+GN P    S  S H  +L++V+ S     D 
Sbjct: 11   LSIISFTLAMLICCCHGSSDNRQVYIVYMGNLPKGEVSISSLHTSMLQQVLPSNIVGSDV 70

Query: 69   LLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVN 128
            LL+SY RSFNGFA +LT++EA+K++ KDGVVSVF + K+ LHT+RSWDFMGF   V R +
Sbjct: 71   LLYSYHRSFNGFAAKLTKDEAEKLKEKDGVVSVFLSQKRQLHTSRSWDFMGFNRKVKR-S 130

Query: 129  QVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDN 188
              ESDI++G+LD+GIWPES SF+D  +G +PAKWKG CQ S NF CN+KIIGA+ Y++D 
Sbjct: 131  VFESDIIIGMLDTGIWPESQSFNDTEFGAIPAKWKGTCQTSANFTCNRKIIGAKYYKADG 190

Query: 189  NFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSD 248
            +F P +++SPRDS+GHG+HT+ST AGGLVS+ASLYGLA GTARGGVPSARIAVYKICWS 
Sbjct: 191  DFDPSEYQSPRDSEGHGSHTSSTAAGGLVSKASLYGLAKGTARGGVPSARIAVYKICWSI 250

Query: 249  GCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGND 308
            GC D DILAAFDDAIADGVDIISLSVG   P  YF+D+IAIGAFHSMK+GILTSNSAGN 
Sbjct: 251  GCDDVDILAAFDDAIADGVDIISLSVGSIFPSEYFDDTIAIGAFHSMKNGILTSNSAGNS 310

Query: 309  GPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGH 368
            GP   TI NFSPWSLSVAAS+IDRK V+ V+LGN  I++G +INTFDL+GK YP I    
Sbjct: 311  GPSLSTITNFSPWSLSVAASTIDRKFVTRVKLGNGEIYEGTSINTFDLKGKMYPFIAGAA 370

Query: 369  APNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFAS---LSSAVGVVMNNAG 428
            APN S G++   SR+C   ++D  LVKGKI+ CD     +T+      + AVGVV+ +  
Sbjct: 371  APNTSQGYTSDDSRFCGPGTLDETLVKGKIVFCDY----TTYIEGPVEAGAVGVVVQSGR 430

Query: 429  VKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPN 488
             KD A +Y LP S L    G +V NY+     PTATIFK+   +D  AP VV+FSSRGPN
Sbjct: 431  YKDYAFTYGLPLSNLNLNVGRNVLNYVNLTENPTATIFKTTVEDDQFAPFVVTFSSRGPN 490

Query: 489  PETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYV 548
            P T DILKPD+TAPGV+ILAAW+   P+S    D+R   YNIISGTSMSCPHAT AA YV
Sbjct: 491  PATPDILKPDVTAPGVDILAAWSEAVPLSDTEDDTRIVPYNIISGTSMSCPHATGAAAYV 550

Query: 549  KTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANETDYV 608
            K+FHPTWSPAAIKSALMTTA P++ E N +AEFAYGAGHI+P +A  PGL+YDA E DYV
Sbjct: 551  KSFHPTWSPAAIKSALMTTAFPMSSENNLEAEFAYGAGHINPAQAAQPGLVYDAGEIDYV 610

Query: 609  NFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNV 668
             FLCGQGY+   ++ +T     C+   +G VWDLNYPSF LST+P  SI + F RT+ NV
Sbjct: 611  KFLCGQGYTPKQLKLITESNFTCSEETNGTVWDLNYPSFTLSTSPGNSITRVFHRTVMNV 670

Query: 669  GSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASVVWSDG 728
            GS  STY + ++ AP GL I V P VLSF  +G K +F +T+G  I  S++S S++W DG
Sbjct: 671  GSPVSTYKA-VVNAPPGLIIQVQPSVLSFKSLGQKLTFVVTVGAEIGNSMISGSLIWDDG 730

Query: 729  SHN-----------VYVVYMGTSFEPDYSTASKQHINMLQQVLTISD-ASKSLIYSYHRS 788
             H            VY+VYMG   +   ST S  H +ML +VL  S   S+ L+YSYHRS
Sbjct: 731  VHQVRSPIVAYASLVYIVYMGNLPKGKVSTTS-LHTSMLHEVLPSSTVGSEVLLYSYHRS 790

Query: 789  FSGFAATLNDEEARKLAEMDGVVSMFVSEKKHLQTTRSWDFMGLFEGAPRTKLETDLIIG 848
            F+GFAA L  +EA KL E +GVVS+F S+KK L TTRSWDFMG  +   R+ +E+D+I+G
Sbjct: 791  FNGFAAKLTKDEAAKLREKEGVVSVFRSQKKQLHTTRSWDFMGFNKKVKRSIIESDIIVG 850

Query: 849  ILDTGIWPESESFSDEGFDPPPAKWKGECQSSYNFTCNNKIIGARSFRSGATINCEHDNL 908
            +LDTGIWPES+SF D G  P P KWKG CQSS NFTCN KIIGA+ +R+   I   +D  
Sbjct: 851  MLDTGIWPESQSFDDTGLGPIPQKWKGTCQSSANFTCNRKIIGAKYYRAKGDI--PYDYK 910

Query: 909  SPRDTEGHGTLTSSTAAGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADIL 968
            +PRD EGHGT TSSTAAG LV+ ASL+G+  GT+RGG PSARIAVYKICWS+GC+D DIL
Sbjct: 911  TPRDYEGHGTHTSSTAAGGLVSKASLYGIAKGTARGGVPSARIAVYKICWSDGCYDEDIL 970

Query: 969  AAFDHAIADGVDIISLSVAGSDLKDYFEDSIAIGAFHAMKSGILTSNAGGNTGPLFGTIQ 1028
            AAFD AIADGVDIISLSV      +YF+D+IAIGAFH+MK+G+LTSN+ GN+GP+  +I 
Sbjct: 971  AAFDDAIADGVDIISLSVGSFFASEYFDDTIAIGAFHSMKNGVLTSNSAGNSGPILSSIT 1030

Query: 1029 NVSPWSLSVAASTTDRKFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGF 1088
            N SPWSLSVAAST DRKF+T VKLGNG  + G S+NTF L  K++P I    APNT+ G+
Sbjct: 1031 NFSPWSLSVAASTIDRKFITKVKLGNGKIYEGTSINTFDLKGKMYPFIAGAAAPNTSQGY 1090

Query: 1089 NGSSSRFCFNGSLDVNLIQGKIVLCDGIAD--GEVSSGAVGMIMQDDTLQEAALLFPLPA 1148
            +   SRFC  G+L+  L++GKIV CD  +D  G   +GA+G + Q    ++    + LP 
Sbjct: 1091 SPEGSRFCGPGTLNETLVKGKIVFCDYDSDGTGPAEAGAIGAVFQSGIYKDYVFPYSLPL 1150

Query: 1149 SQLDFNAGKNVFQYMRSTSNPVASIEKSTATKDLLAPYVVSFSSRGPNLLTPDILKPDLA 1208
            S L+ + G+NV  Y+ +T  P A+I K+    D  AP+VVSFSSRGPN  TPDILKPD+ 
Sbjct: 1151 SNLNLDDGRNVLNYVNTTEKPTATIFKTNVVDDQFAPFVVSFSSRGPNPATPDILKPDVT 1210

Query: 1209 APGVDILASWPEGSAITGFTGDNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAAI 1268
            APGVDILA+W E   +T    D R  P+NI+SGTS++CPHATGAAAYVKSFHPTWSPAAI
Sbjct: 1211 APGVDILAAWSEAVPLTETKDDTRIVPYNIISGTSMSCPHATGAAAYVKSFHPTWSPAAI 1270

Query: 1269 KSALMTTALPMTPKVNAEAEFAYGAGHLNPVNATNPGLVYDAQYIDYIEFLCGQGYSTEN 1328
            KSALMTTA PM+ K N EAEFAYGAGH+NP  A  PGLVYDA  IDY++FLCGQGY+ + 
Sbjct: 1271 KSALMTTAFPMSSKNNLEAEFAYGAGHINPAQAAQPGLVYDAGEIDYVKFLCGQGYTPKQ 1330

Query: 1329 LRLVTGDQINCSDVRKTAASHLNYPSFALLIESQRLATCIYHRTVTNVGLSVSTYKAVIQ 1388
            L L+T     CS+    A   LNYPSF L        T ++HRTVTNVG  VSTYK V+ 
Sbjct: 1331 LNLITESNFTCSEETNGAVWDLNYPSFTLSTTPGNSITRVFHRTVTNVGSPVSTYKVVVN 1390

Query: 1389 APSGLKVRVYPTTLSFSSLGQKISFTVRVEVESDEKMLSGSLTWDDGLHLVRSPIVAY 1429
            AP GL ++V P+ LSF SLGQK++F V V  E    M+SGSL WDDG+H VRSPIVAY
Sbjct: 1391 APPGLIIQVQPSVLSFKSLGQKLTFIVTVGAEIGNSMISGSLIWDDGVHQVRSPIVAY 1439

BLAST of CmaCh04G001830 vs. ExPASy TrEMBL
Match: A0A5D3CCZ5 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001930 PE=3 SV=1)

HSP 1 Score: 1624.8 bits (4206), Expect = 0.0e+00
Identity = 836/1440 (58.06%), Postives = 1052/1440 (73.06%), Query Frame = 0

Query: 3    SSVIFKLVFLGFFGCLLASSLDSDNDG-RKVYIVYLGNKPDDMASTPSHHMRLLEEVVGS 62
            SS+I +L+FL F   LL  S  S N+G +K YIVY+G+ P    ST SHH+RLL+E +GS
Sbjct: 2    SSLIPRLLFLNFCLFLLFFSSSSQNNGSQKTYIVYMGSHPKGKVSTSSHHIRLLKETIGS 61

Query: 63   TFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTT 122
            +F P +LLHS+KRSFNGF  +LTE EA+K+   +GV+SVFPNGKK LHTTRSWDFMGF+ 
Sbjct: 62   SFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSE 121

Query: 123  NVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGAR 182
             V RV  VES+++VGVLDSGIWPESPSF   GYGP PAKWKG+C+ S NF CN KIIGAR
Sbjct: 122  QVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSCNNKIIGAR 181

Query: 183  AYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVY 242
            +YRS+  +P  D + PRDSDGHGTHTAS VAGGLV QA++ GL LGTARGGVPSARIA Y
Sbjct: 182  SYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGGVPSARIAAY 241

Query: 243  KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTS 302
            K+CWSDGC DADILAAFDDAIADGVDIIS S+GGS  + YFNDSIAIG+FH+MK GILTS
Sbjct: 242  KVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTS 301

Query: 303  NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYP 362
             + GN+GPD+ TI NFSPWSLSVAAS+ DRK  + V+LG+   F G ++NTFD++GK  P
Sbjct: 302  LAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSVNTFDIKGKQIP 361

Query: 363  LIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMN 422
            L+YAG  P     F  S SR C +N++D  LVKGKI+VCDS+  P   A++  AVG++M 
Sbjct: 362  LVYAGDIP--KAPFDSSVSRLCFENTIDLKLVKGKIVVCDSLTVPGGVAAVKGAVGIIMQ 421

Query: 423  NAGVKDNARSYPLPSSYLTRAAGNHVKNYIGS-NNFPTATIFKSNEVNDTAAPIVVSFSS 482
            +    D+  S+P+P+S+L   AG  + +YI S N+ PTATI KS E     AP V SFSS
Sbjct: 422  DDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIPTATIKKSTERKRKRAPSVASFSS 481

Query: 483  RGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAA 542
            RGPNP T +ILKPDL+ PGVEILAAW+PI+P S   +DS+  LYNIISGTSM+CPH TAA
Sbjct: 482  RGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACPHVTAA 541

Query: 543  AVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANE 602
            A YVK+FHPTWSP+A+KSAL+TTA  ++ + N   EF YGAGHI+PL A++PGLIYDA+E
Sbjct: 542  AAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLIYDASE 601

Query: 603  TDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRT 662
             DYV+FLCGQGY+T ++++++ D + C++ NS  V+DLNYPSFALST  S+ INQ + RT
Sbjct: 602  IDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQVYRRT 661

Query: 663  LTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASVV 722
            +TNVGS+++TY + I+   + L I V+P VLSF  +G ++SF +TI G I ++I SAS+V
Sbjct: 662  VTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQSFEVTIKGKIRKNIESASLV 721

Query: 723  WSDGSHNV------YVVYMGTSFEPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFS 782
            W DG H V      Y+VYMG   +   ST S  H+ +L++ +  S   KSL++S+ RSF+
Sbjct: 722  WDDGKHKVRSPITTYIVYMGNHPKGKPSTPS-HHMRLLKETIGSSFPPKSLLHSFKRSFN 781

Query: 783  GFAATLNDEEARKLAEMDGVVSMFVSEKKHLQTTRSWDFMGLFEGAPRT-KLETDLIIGI 842
            GF A + +EEA+K++EM+GV+S+F + KK L TTRSWDFMG  E   R   +E+D+I+G+
Sbjct: 782  GFVAKMTEEEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGV 841

Query: 843  LDTGIWPESESFSDEGFDPPPAKWKGECQSSYNFTCNNKIIGARSFRSGATINCEHDNLS 902
             DTGIWPES SF D G+ PPPAKWKG C+ S NF+CNNKIIGARS+ S    + E D   
Sbjct: 842  FDTGIWPESPSFDDTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQ-DLEGDIKG 901

Query: 903  PRDTEGHGTLTSSTAAGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILA 962
            PRD++GHGT T+ST AG LV  A++ GLG GT+RGG PSARIAVYK+CWS+GC DADILA
Sbjct: 902  PRDSDGHGTHTASTVAGGLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILA 961

Query: 963  AFDHAIADGVDIISLSVAGSDLKDYFEDSIAIGAFHAMKSGILTSNAGGNTGPLFGTIQN 1022
            AFD AIADGVDIIS S+      +YF DS+AIG+FHAMK GIL+S A GNTGP F TI N
Sbjct: 962  AFDDAIADGVDIISGSLGAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIAN 1021

Query: 1023 VSPWSLSVAASTTDRKFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGFN 1082
             SPWSL+VAASTTDR F T V+LG+G    G S+NTF +  K  PL+Y GD P   A  +
Sbjct: 1022 YSPWSLTVAASTTDRVFETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPK--ANIS 1081

Query: 1083 GSSSRFCFNGSLDVNLIQGKIVLCDGIADGE---VSSGAVGMIMQDDTLQEAALLFPLPA 1142
             S SR C   S+D+ L +GKIV+CD + D        GAVG+IMQDD+  +    +P+PA
Sbjct: 1082 SSLSRECVENSVDLELAKGKIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGH-SYPIPA 1141

Query: 1143 SQLDFNAGKNVFQYMRSTSN-PVASIEKSTATKDLLAPYVVSFSSRGPNLLTPDILKPDL 1202
            S +D  +G  +  Y+ ST++ P A+I+KST  K   AP V SFSSRGPN +T +ILKPDL
Sbjct: 1142 SHVDSKSGAVILSYINSTNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDL 1201

Query: 1203 AAPGVDILASWPEGSAITGFTGDNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAA 1262
            +APGV+ILA+WP  S+ +G   DN++  +NI+SGTS+ACPH T  AAYVKSFHPTWSPAA
Sbjct: 1202 SAPGVEILAAWPPISSPSGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAA 1261

Query: 1263 IKSALMTTALPMTPKVNAEAEFAYGAGHLNPVNATNPGLVYDAQYIDYIEFLCGQGYSTE 1322
            +KSALMTTA PM+PK N E EFAYGAGHLNP+ A +PGL+YDA   DY+ FLCGQGY+TE
Sbjct: 1262 LKSALMTTAFPMSPKRNKEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTE 1321

Query: 1323 NLRLVTGDQINCSDVRKTAASHLNYPSFALLIESQRLATCIYHRTVTNVGLSVSTYKAVI 1382
             L+LV+ D   CS         LNYPSFAL   +      +Y RTVTNVG   +TYKA I
Sbjct: 1322 LLQLVSEDNNTCSSNDSDTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATI 1381

Query: 1383 QAP-SGLKVRVYPTTLSFSSLGQKISFTVRVEVESDEKMLSGSLTWDDGLHLVRSPIVAY 1429
              P   L+++V P+ LSF+ LG++ SF V ++ +    + S SL WDDG H VRSPI  +
Sbjct: 1382 INPWKNLEIKVNPSVLSFNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 1434

BLAST of CmaCh04G001830 vs. ExPASy TrEMBL
Match: A0A7J9F6D3 (Uncharacterized protein OS=Gossypium trilobum OX=34281 GN=Gotri_004915 PE=3 SV=1)

HSP 1 Score: 1617.8 bits (4188), Expect = 0.0e+00
Identity = 839/1432 (58.59%), Postives = 1029/1432 (71.86%), Query Frame = 0

Query: 3    SSVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVV-GS 62
            S +IF L    +F           +D R+VYIVY+GN P    ST S H  +L EV+  S
Sbjct: 26   SLIIFTLSMFMWFS-------HGSSDKRQVYIVYMGNLPKGKVSTTSLHTSMLHEVLPSS 85

Query: 63   TFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTT 122
            T   + LL+SY RSFNGFA +LT++EA K+R KDGVVSVF + KK LHTTRSWDFMGF  
Sbjct: 86   TVGSEVLLYSYHRSFNGFAAKLTKDEAAKLREKDGVVSVFRSQKKQLHTTRSWDFMGFNK 145

Query: 123  NVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGAR 182
             V R + +ESDI+VG+LD+GIWPES SF+D G GP+P KWKG CQ+S NF CN+KIIGA+
Sbjct: 146  KVKR-SIIESDIIVGMLDTGIWPESQSFNDTGLGPIPIKWKGTCQSSANFTCNRKIIGAK 205

Query: 183  AYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVY 242
             YR++ +  P D++SPRDS+GHGTHT+ST AGGLVS+ASLYG+A GTARGGVPSARIAVY
Sbjct: 206  YYRANGDISPYDYKSPRDSEGHGTHTSSTAAGGLVSKASLYGIAKGTARGGVPSARIAVY 265

Query: 243  KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTS 302
            KICWSDGCYD DILAAFDDAIADGVDIISLSVG      YF+D+IAIGAFHSMK+G+LTS
Sbjct: 266  KICWSDGCYDEDILAAFDDAIADGVDIISLSVGSIFSSEYFDDTIAIGAFHSMKNGVLTS 325

Query: 303  NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYP 362
            NSAGN GP   +I NFSPWSLSVAAS+IDRK ++ V+LGN  I++G +INTFDL+GK YP
Sbjct: 326  NSAGNSGPILSSITNFSPWSLSVAASTIDRKFITKVKLGNGKIYEGTSINTFDLKGKMYP 385

Query: 363  LIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMN 422
             I    APN S G++   SR+C   +++  LVKGKI+ CD   S  T  + + A+G V  
Sbjct: 386  FIAGAAAPNTSQGYTSEDSRFCGPGTLNETLVKGKIVFCD-YDSDGTGPAQAGAIGAVFQ 445

Query: 423  NAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSR 482
            + G KD    Y LP S L    G +V NY+ +   PTATIFK+N  +D  AP VVSFSSR
Sbjct: 446  SGGYKDYVFPYSLPLSNLNLDDGRNVLNYVNTTENPTATIFKTNVEDDQFAPFVVSFSSR 505

Query: 483  GPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAA 542
            GPNP T DILKPD+TAPGV+ILAAW+ + P++    D+R   YNIISGTSMSCPHAT AA
Sbjct: 506  GPNPATPDILKPDVTAPGVDILAAWSEVVPLTETKDDTRIVAYNIISGTSMSCPHATGAA 565

Query: 543  VYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANET 602
             YVK+FHPTWSPAAIKSALMTTA P++ + N +AEFAYGAGHI+P +A  PGL+YDA E 
Sbjct: 566  AYVKSFHPTWSPAAIKSALMTTAFPMSSKNNLEAEFAYGAGHINPAQAAQPGLVYDAGEI 625

Query: 603  DYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTL 662
            DYV FLCGQGY+   ++ +T     C+   +G VWDLNYPSF LS TP  SI + F RT+
Sbjct: 626  DYVKFLCGQGYTPKQLKLITESNFTCSEETNGAVWDLNYPSFTLSATPGNSITRVFHRTV 685

Query: 663  TNVGSEASTYTSKILG--APEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASV 722
            TNVGS  STY   +    AP+  T+      L++  +     FTL++  + S        
Sbjct: 686  TNVGSPVSTYKVVVNAPPAPQNSTMAARTSPLAWLSL---VIFTLSMFMSCSHG------ 745

Query: 723  VWSDGSHNVYVVYMGTSFEPDYSTASKQHINMLQQVLTISD-ASKSLIYSYHRSFSGFAA 782
              S  +  VY+VYMG+  +   ST S  H +ML +VL  +   S  L+YSYHRSF+GFAA
Sbjct: 746  --SSDNRKVYIVYMGSLPKGKVSTTS-LHTSMLHEVLPGNTVGSDVLLYSYHRSFNGFAA 805

Query: 783  TLNDEEARKLAEMDGVVSMFVSEKKHLQTTRSWDFMGLFEGAPRTKLETDLIIGILDTGI 842
             L  +EA KL   DGVVS+F+S++K L T+RSWDFMGL     R+ +E+D+I+G+LDTGI
Sbjct: 806  KLTKDEAAKLRGKDGVVSVFLSQRKQLHTSRSWDFMGLNRKVKRSVIESDIIVGMLDTGI 865

Query: 843  WPESESFSDEGFDPPPAKWKGECQSSYNFTCNNKIIGARSFRSGATINCEHDNLSPRDTE 902
            WPES+SF+D GF P P KW+G CQSS NFTCNNKIIGAR +R+    +  +D +SPRD+E
Sbjct: 866  WPESQSFNDTGFGPIPRKWRGTCQSSTNFTCNNKIIGARYYRANGDYS-PYDYISPRDSE 925

Query: 903  GHGTLTSSTAAGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHA 962
            GHGT TSSTAAG LV+ ASL+GL  GT+RGG PSARIAVYKICW +GC+D DILAAFD A
Sbjct: 926  GHGTHTSSTAAGGLVSKASLYGLAKGTARGGVPSARIAVYKICWYDGCYDEDILAAFDDA 985

Query: 963  IADGVDIISLSVAGSDLKDYFEDSIAIGAFHAMKSGILTSNAGGNTGPLFGTIQNVSPWS 1022
            IADGVDIISLSV      DYF+D+IAIGAFH+MK+GILTSN+ GN+GP   +I N SPWS
Sbjct: 986  IADGVDIISLSVGSIFWSDYFDDTIAIGAFHSMKNGILTSNSAGNSGPSPSSITNFSPWS 1045

Query: 1023 LSVAASTTDRKFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGFNGSSSR 1082
            LSVAAST DRKFVT VKLGNG  + G S+NTF L  K++P I    APNT+ G+    SR
Sbjct: 1046 LSVAASTIDRKFVTKVKLGNGVIYEGTSINTFDLKGKMYPFIAGAAAPNTSQGYTSEDSR 1105

Query: 1083 FCFNGSLDVNLIQGKIVLC--DGIADGEVSSGAVGMIMQDDTLQEAALLFPLPASQLDFN 1142
            +C  G+LD  L++GKIV C  D  ADG V +GA+G + Q    ++ A  + LP S L+ +
Sbjct: 1106 YCGPGTLDETLVKGKIVFCDYDSNADGPVEAGAIGAVFQSGRHKDYAFAYGLPLSNLNLD 1165

Query: 1143 AGKNVFQYMRSTSNPVASIEKSTATKDLLAPYVVSFSSRGPNLLTPDILKPDLAAPGVDI 1202
             G+ VF Y+ +T NP A+I K+    +  AP+VVSFSSRGPN +T DILKPDL APGVDI
Sbjct: 1166 DGRIVFDYVNTTENPTATIFKTNVEDNQFAPFVVSFSSRGPNPVTADILKPDLTAPGVDI 1225

Query: 1203 LASWPEGSAITGFTGDNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMT 1262
            LA+W E   +T    D R   +NI+SGTS++CPHATGAAAYVKSFHPTWSPAAIKSALMT
Sbjct: 1226 LAAWSEALPLTETEDDTRVVSYNIISGTSMSCPHATGAAAYVKSFHPTWSPAAIKSALMT 1285

Query: 1263 TALPMTPKVNAEAEFAYGAGHLNPVNATNPGLVYDAQYIDYIEFLCGQGYSTENLRLVTG 1322
            TA PM+ + N EAEFAYGAGH+NP  A  PGL+YDA  IDY++FLCGQGYS + ++L+T 
Sbjct: 1286 TAFPMSSENNIEAEFAYGAGHINPALAARPGLIYDAGEIDYVKFLCGQGYSPKQIKLITE 1345

Query: 1323 DQINCSDVRKTAASHLNYPSFALLIESQRLATCIYHRTVTNVGLSVSTYKAVIQAPSGLK 1382
                CS+V   A   LNYPSFAL        T ++HRTVTNVG  VSTYKAV+ AP GL 
Sbjct: 1346 SNTKCSEVMNEAVWDLNYPSFALSTTPGDSVTRVFHRTVTNVGSPVSTYKAVVNAPPGLI 1405

Query: 1383 VRVYPTTLSFSSLGQKISFTVRVEVESDEKMLSGSLTWDDGLHLVRSPIVAY 1429
            ++V P+ LSF SLGQK SFTV +  +    M+SGSL WDDG+H VRSP+VAY
Sbjct: 1406 IQVEPSVLSFKSLGQKQSFTVTIGAQLGNSMVSGSLIWDDGVHEVRSPVVAY 1435

BLAST of CmaCh04G001830 vs. ExPASy TrEMBL
Match: A0A1R3I1N2 (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_25554 PE=3 SV=1)

HSP 1 Score: 1591.2 bits (4119), Expect = 0.0e+00
Identity = 822/1422 (57.81%), Postives = 1021/1422 (71.80%), Query Frame = 0

Query: 32   VYIVYLGNKPDDMASTPSHHMRLLEEVVGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKI 91
            VYIVY+G+ P    S  + H ++L+EVV S+ S DALL+SY+RSFNGFA +LTE+EA+K+
Sbjct: 22   VYIVYMGDLPKGELSASTLHTKMLQEVVPSSPS-DALLYSYQRSFNGFAAKLTEQEAEKL 81

Query: 92   RAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVGVLDSGIWPESPSFSD 151
            R K+ VVSVF + KK L T+RSWDFMGF     R + +ES+I+VG+LDSGIWPES SF+D
Sbjct: 82   RGKEEVVSVFLSKKKKLLTSRSWDFMGFPIKKVRRSTMESEIIVGMLDSGIWPESESFND 141

Query: 152  VGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDFESPRDSDGHGTHTASTV 211
             G+GP+P KWKG+CQ S+NF CNKKIIGA+ YR+   F P D +SPRD  GHGTHTAST 
Sbjct: 142  TGFGPIPNKWKGSCQTSSNFTCNKKIIGAKYYRAIGQFGPHDLKSPRDRGGHGTHTASTA 201

Query: 212  AGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISL 271
            AGGLVSQ S YGLA GT RG VPSARIAVYKICW DGCYD DILAAFDDAIADGVDIISL
Sbjct: 202  AGGLVSQTSFYGLAKGTVRGAVPSARIAVYKICWHDGCYDEDILAAFDDAIADGVDIISL 261

Query: 272  SVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDR 331
            SVG  E   YF DSIAIGAFHSMK+GILTSN+AGN+GP  F+I N SPWSLSVAAS+IDR
Sbjct: 262  SVGNLEASDYFEDSIAIGAFHSMKNGILTSNAAGNEGPSLFSIINISPWSLSVAASTIDR 321

Query: 332  KLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRN 391
               + V+LGN  IF+G +INTFDL+GK YP+I+ G+APNIS G +   SRYC  +S++  
Sbjct: 322  NFATKVKLGNGAIFEGSSINTFDLKGKMYPMIWGGNAPNISSGATSEESRYCWPDSLNAT 381

Query: 392  LVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYI 451
            LVKGKI+ C+ + S ++ A  + AVG V       D A SYPLP + L    G  V NY+
Sbjct: 382  LVKGKIVFCEDLESNASEAVDAGAVGAVFLYGQATDYAFSYPLPYANLNIDDGRIVLNYL 441

Query: 452  GSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAP 511
             +    TATI+KS++ N+  AP VVSFSSRGPNP T DILKPDLTAPGV+ILAAW+P+ P
Sbjct: 442  NTTEDTTATIYKSDQ-NNQFAPYVVSFSSRGPNPITADILKPDLTAPGVDILAAWSPLNP 501

Query: 512  VSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVEL 571
             S   +D+R   YNIISGTSMSCPHAT +A YVK+ HPTWSPAAIKSALMTTA P++ E 
Sbjct: 502  PSFYQEDTRKVPYNIISGTSMSCPHATGSAAYVKSLHPTWSPAAIKSALMTTAFPMSSEN 561

Query: 572  NPQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAAN 631
            N ++EFAYGAGHI+P +A  PGL+YDA E DYV FLCGQGY+   ++ +TG  S C+A  
Sbjct: 562  NLESEFAYGAGHINPAQAAQPGLVYDAGEIDYVKFLCGQGYTPKNMQLITGSNSSCSAEI 621

Query: 632  SGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVL 691
            +G VWDLNYPSFALST   +SI + F RT+TNVGS  S+Y + I+ AP GL I V P +L
Sbjct: 622  NGTVWDLNYPSFALSTKVGKSITRVFHRTVTNVGSPMSSYKA-IVNAPPGLIIQVQPSLL 681

Query: 692  SFNGIGHKESFTLTIGGTISQSIVSASVVWSDGSHN------------------------ 751
            SF  +G  +SF +T+   I  S++S S+ W DG H                         
Sbjct: 682  SFKSLGESQSFVVTVHAEIGNSMISGSLTWDDGWHKVLSRASRICNKNIVTAGFGKLLFC 741

Query: 752  ----VYVVYMGTSFEPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFSGFAATLNDE 811
                VY+VYMG   + ++S A   H  M+Q+V++ S +   L+YSY RSF+GFAA L  E
Sbjct: 742  CHLAVYIVYMGDLPKGEFS-APSLHTKMVQEVVS-SASPDILLYSYQRSFNGFAAKLTKE 801

Query: 812  EARKLAEMDGVVSMFVSEKKHLQTTRSWDFMGL-FEGAPRTKLETDLIIGILDTGIWPES 871
            EA+KL E +GVVS+F+S+K+ L TTRSWDFMG       R+ +E+D+++G++DTGIWPES
Sbjct: 802  EAQKLKENEGVVSVFLSQKRQLHTTRSWDFMGFPVSKVKRSVIESDIVVGMIDTGIWPES 861

Query: 872  ESFSDEGFDPPPAKWKGECQSSYNFTCNNKIIGARSFRSGATINCEHDNLSPRDTEGHGT 931
            ESF D GF P P KWKG CQ + NFTCN KIIGA+ + +G  I+  +D +SPRD +GHGT
Sbjct: 862  ESFDDTGFGPIPQKWKGSCQKASNFTCNKKIIGAKYYHAGGKIDPRYDFVSPRDNDGHGT 921

Query: 932  LTSSTAAGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHAIADG 991
             T+STAAG LV+  SL+GL  G +RGG PSARIAVYK+CWS+GC D D+LAAFD AIADG
Sbjct: 922  HTTSTAAGGLVSHTSLYGLAEGIARGGVPSARIAVYKVCWSDGCSDLDLLAAFDDAIADG 981

Query: 992  VDIISLSVAGSDLKDYFEDSIAIGAFHAMKSGILTSNAGGNTGPLFGTIQNVSPWSLSVA 1051
            VDIIS+S+      DYF+D IAIGAFH+MK+GILTSN+ GN+GP+  T+ N+SPWSLSVA
Sbjct: 982  VDIISISIGLPFAIDYFDDVIAIGAFHSMKNGILTSNSAGNSGPIPFTVSNLSPWSLSVA 1041

Query: 1052 ASTTDRKFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGFNGSSSRFCFN 1111
            AST DR F T VKLGNG+ + G S+NTF L  K++P I+ GDAP+T+       SRFC  
Sbjct: 1042 ASTIDRNFGTKVKLGNGEIYEGSSINTFDLEGKMYPFIWGGDAPDTSDDNTWDDSRFCAY 1101

Query: 1112 GSLDVNLIQGKIVLC---DGIADGEVSSGAVGMIMQDDTLQEAALLFPLPASQLDFNAGK 1171
            G+L+  L++GKIV C   D    G  ++GAVG++ QD   ++    +PLP ++L+   G+
Sbjct: 1102 GTLNKTLVKGKIVFCEAGDNDPFGPFAAGAVGVVFQDGQAKDYEFTYPLPLAKLNMEDGR 1161

Query: 1172 NVFQYMRSTSNPVASIEKSTATKDLLAPYVVSFSSRGPNLLTPDILKPDLAAPGVDILAS 1231
             V  Y+ +T +  A+I KS    +  APYVVSFSSRGPN +T D+LKPDL APGV+ILA+
Sbjct: 1162 IVLNYLNTTKDTTATIYKSDQENNQFAPYVVSFSSRGPNSITEDLLKPDLTAPGVNILAA 1221

Query: 1232 WPEGSAITGFTGDNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTAL 1291
            W     ++G+  D RK  +NI+SGTS+ACPHATGAAAYVKS HPTWS AAIKSA+MTTA+
Sbjct: 1222 WAPVDPLSGYEDDPRKVQYNIISGTSMACPHATGAAAYVKSLHPTWSAAAIKSAIMTTAM 1281

Query: 1292 PMTPKVNAEAEFAYGAGHLNPVNATNPGLVYDAQYIDYIEFLCGQGYSTENLRLVTGDQI 1351
            PM+ + N E EFAYGAGH+NP  A  PGLVYDA  ID+++FLCGQGYS + L+L+TG   
Sbjct: 1282 PMSAQNNIEREFAYGAGHINPARAAEPGLVYDAGEIDFVKFLCGQGYSPKMLQLITGSSN 1341

Query: 1352 NCSDVRKTAASHLNYPSFALLIESQRLATCIYHRTVTNVGLSVSTYKAVIQAPSGLKVRV 1411
            +CS         LNYPSFAL        T ++HRTVTNVG  VSTYKAVI AP GL ++V
Sbjct: 1342 SCSAETNGNVWDLNYPSFALSTTPGNPVTGVFHRTVTNVGSEVSTYKAVINAPPGLSIQV 1401

Query: 1412 YPTTLSFSSLGQKISFTVRVEVESDEKMLSGSLTWDDGLHLV 1422
             P+ LSF SLG+  SF V V  E    MLSGSLTWDDG H V
Sbjct: 1402 QPSVLSFKSLGESQSFVVTVHAEVGNSMLSGSLTWDDGQHQV 1438

BLAST of CmaCh04G001830 vs. NCBI nr
Match: KAG7030696.1 (hypothetical protein SDJN02_04733, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2755.3 bits (7141), Expect = 0.0e+00
Identity = 1378/1424 (96.77%), Postives = 1400/1424 (98.31%), Query Frame = 0

Query: 9    LVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGSTFSPDAL 68
            LVFLGFFGCLLASSLDS NDGRKVYIVYLGNKPDDMASTPSHHMRLLE+VVGSTF+PDAL
Sbjct: 733  LVFLGFFGCLLASSLDSVNDGRKVYIVYLGNKPDDMASTPSHHMRLLEQVVGSTFAPDAL 792

Query: 69   LHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQ 128
            LHSYKRSFNGF VRLTE EAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQ
Sbjct: 793  LHSYKRSFNGFVVRLTEGEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQ 852

Query: 129  VESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNN 188
            VESDIVVGVLDSGIWPESPSFSDVGYGP PAKWKGACQAS NFRCNKKIIGARAYRSDNN
Sbjct: 853  VESDIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQASTNFRCNKKIIGARAYRSDNN 912

Query: 189  FPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDG 248
            FPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDG
Sbjct: 913  FPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDG 972

Query: 249  CYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDG 308
            CYDADILAAFDDAIADGVDIISLSVGGSEP+YYFNDSIAIGAFHSMKHGILTSNSAGNDG
Sbjct: 973  CYDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSNSAGNDG 1032

Query: 309  PDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHA 368
            PDYFTIRNFSPWSLSVAASSIDRKLVS VQLGN NIFQGYTINTFDLQGK YPLIYAGHA
Sbjct: 1033 PDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGYTINTFDLQGKQYPLIYAGHA 1092

Query: 369  PNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDN 428
            PNISGGF+GSSSRYCS+NSVDRNLVKGKI+VCDSILSPSTF SLSSAVGVVMN+AG+KDN
Sbjct: 1093 PNISGGFTGSSSRYCSQNSVDRNLVKGKIVVCDSILSPSTFVSLSSAVGVVMNDAGLKDN 1152

Query: 429  ARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETY 488
            ARSYPLPSSYLTRAAGNHVKNY+GSNNFPTATI KSNEVNDT+APIVVSFSSRGPNPET 
Sbjct: 1153 ARSYPLPSSYLTRAAGNHVKNYMGSNNFPTATILKSNEVNDTSAPIVVSFSSRGPNPETN 1212

Query: 489  DILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFH 548
            DILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFH
Sbjct: 1213 DILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFH 1272

Query: 549  PTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLC 608
            PTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKA NPGL+YDANETDYVNFLC
Sbjct: 1273 PTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLC 1332

Query: 609  GQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEA 668
            GQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEA
Sbjct: 1333 GQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEA 1392

Query: 669  STYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASVVWSDGSHNV 728
            STYTSKILGAPEGLTITVDPP LSFN IG K+SFTLTI GTISQSIVSASVVWSDGSHNV
Sbjct: 1393 STYTSKILGAPEGLTITVDPPALSFNSIGDKKSFTLTIDGTISQSIVSASVVWSDGSHNV 1452

Query: 729  YVVYMGTSFEPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKL 788
            YVVYMGTSFEPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKL
Sbjct: 1453 YVVYMGTSFEPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKL 1512

Query: 789  AEMDGVVSMFVSEKKHLQTTRSWDFMGLFEGAPRTKLETDLIIGILDTGIWPESESFSDE 848
             EMDGVVSMFVSEKKHLQTTRSWDFMGLFE APRT+LE++LIIGILDTGIWPESESFSDE
Sbjct: 1513 VEMDGVVSMFVSEKKHLQTTRSWDFMGLFERAPRTRLESELIIGILDTGIWPESESFSDE 1572

Query: 849  GFDPPPAKWKGECQSSYNFTCNNKIIGARSFRSGATINCEHDNLSPRDTEGHGTLTSSTA 908
            GF PPPAKWKGECQSSYNFTCNNKIIGARSFRSGA INCEHDNLSPRDTEGHGTLTSSTA
Sbjct: 1573 GFGPPPAKWKGECQSSYNFTCNNKIIGARSFRSGAIINCEHDNLSPRDTEGHGTLTSSTA 1632

Query: 909  AGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHAIADGVDIISL 968
            AGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHAIADGVDIISL
Sbjct: 1633 AGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHAIADGVDIISL 1692

Query: 969  SVAGSDLKDYFEDSIAIGAFHAMKSGILTSNAGGNTGPLFGTIQNVSPWSLSVAASTTDR 1028
            SVAGSDLKDYFEDSIAIGAFHAMK+GILTSNAGGNTGPLFGTIQNVSPWSLSVAAST DR
Sbjct: 1693 SVAGSDLKDYFEDSIAIGAFHAMKNGILTSNAGGNTGPLFGTIQNVSPWSLSVAASTIDR 1752

Query: 1029 KFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGFNGSSSRFCFNGSLDVN 1088
            KFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGF+GSSSRFCFNGSLDVN
Sbjct: 1753 KFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGFDGSSSRFCFNGSLDVN 1812

Query: 1089 LIQGKIVLCDGIADGEVSSGAVGMIMQDDTLQEAALLFPLPASQLDFNAGKNVFQYMRST 1148
            LIQGKIVLCDGIADG VSSGAVGMIMQDDTLQEAALLFPLP+SQLDFNAGKNVFQYMRST
Sbjct: 1813 LIQGKIVLCDGIADGVVSSGAVGMIMQDDTLQEAALLFPLPSSQLDFNAGKNVFQYMRST 1872

Query: 1149 SNPVASIEKSTATKDLLAPYVVSFSSRGPNLLTPDILKPDLAAPGVDILASWPEGSAITG 1208
            SNPVASIEKSTATKDLLAPYVVSFSSRGP+LLTPDILKPDLAAPGVDILASWPEGSAITG
Sbjct: 1873 SNPVASIEKSTATKDLLAPYVVSFSSRGPSLLTPDILKPDLAAPGVDILASWPEGSAITG 1932

Query: 1209 FTGDNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAE 1268
            FT DNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAE
Sbjct: 1933 FTWDNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAE 1992

Query: 1269 AEFAYGAGHLNPVNATNPGLVYDAQYIDYIEFLCGQGYSTENLRLVTGDQINCSDVRKTA 1328
            AEFAYGAGHLNPVNATNPGLVYDAQYIDYI+FLCGQGYSTENLRLVTGDQINCSDVRKTA
Sbjct: 1993 AEFAYGAGHLNPVNATNPGLVYDAQYIDYIKFLCGQGYSTENLRLVTGDQINCSDVRKTA 2052

Query: 1329 ASHLNYPSFALLIESQRLATCIYHRTVTNVGLSVSTYKAVIQAPSGLKVRVYPTTLSFSS 1388
            ASHLNYPSFALLI+SQRLATCIY RTVTNVGLSVSTYKAVIQAPSGLKVRVYPTTLSFSS
Sbjct: 2053 ASHLNYPSFALLIKSQRLATCIYRRTVTNVGLSVSTYKAVIQAPSGLKVRVYPTTLSFSS 2112

Query: 1389 LGQKISFTVRVEVESDEKMLSGSLTWDDGLHLVRSPIVAYHMPS 1433
            LGQKISFTVRVEVESDEKMLSGSLTWDDGLHLVRSPIVAYH+PS
Sbjct: 2113 LGQKISFTVRVEVESDEKMLSGSLTWDDGLHLVRSPIVAYHLPS 2156

BLAST of CmaCh04G001830 vs. NCBI nr
Match: KAG6600027.1 (hypothetical protein SDJN03_05260, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2712.2 bits (7029), Expect = 0.0e+00
Identity = 1356/1415 (95.83%), Postives = 1384/1415 (97.81%), Query Frame = 0

Query: 18   LLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGSTFSPDALLHSYKRSFN 77
            ++++S+   +    VYIVYLGNKPDDMASTPSHHMRLLEEVVGSTF+PDALLHSYKRSFN
Sbjct: 721  IVSASVVWSDGSHNVYIVYLGNKPDDMASTPSHHMRLLEEVVGSTFAPDALLHSYKRSFN 780

Query: 78   GFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVGV 137
            GF VRLTE EAQKIRAKDGVVSVF NGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVGV
Sbjct: 781  GFVVRLTEGEAQKIRAKDGVVSVFSNGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVGV 840

Query: 138  LDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDFESP 197
            LDSGIWPESPSFSDVGYGP PAKWKGACQAS NFRCNKKIIGARAYRSDNNFPPEDFESP
Sbjct: 841  LDSGIWPESPSFSDVGYGPPPAKWKGACQASTNFRCNKKIIGARAYRSDNNFPPEDFESP 900

Query: 198  RDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAA 257
            RDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAA
Sbjct: 901  RDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAA 960

Query: 258  FDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNF 317
            FDDAIADGVDIISLSVGGSEP+YYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNF
Sbjct: 961  FDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNF 1020

Query: 318  SPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGGFSG 377
            SPWSLSVAASSIDRKLVS VQLGN NIFQGYTINTFDLQGK YPLIYAGHAPNISGGF+G
Sbjct: 1021 SPWSLSVAASSIDRKLVSKVQLGNNNIFQGYTINTFDLQGKQYPLIYAGHAPNISGGFTG 1080

Query: 378  SSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPLPSS 437
            SSSRYCS+NSVDRNLVKGKI+VCDSILSPSTF SLSSAVGVVMN+AG+KDNARSYPLPSS
Sbjct: 1081 SSSRYCSQNSVDRNLVKGKIVVCDSILSPSTFVSLSSAVGVVMNDAGLKDNARSYPLPSS 1140

Query: 438  YLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPDLTA 497
            YLTRAAGNHVKNY+GSNNFPTATIFKSNEVNDT+APIVVSFSSRGPNPET DILKPDLTA
Sbjct: 1141 YLTRAAGNHVKNYMGSNNFPTATIFKSNEVNDTSAPIVVSFSSRGPNPETNDILKPDLTA 1200

Query: 498  PGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIK 557
            PGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIK
Sbjct: 1201 PGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIK 1260

Query: 558  SALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGYSTAMV 617
            SALMTTAIPLNVELNPQAEFAYGAGHIDPLKA NPGL+YDANETDYVNFLCGQGYSTAMV
Sbjct: 1261 SALMTTAIPLNVELNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMV 1320

Query: 618  RRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEASTYTSKILG 677
            RRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNV +EASTYTSKILG
Sbjct: 1321 RRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVNTEASTYTSKILG 1380

Query: 678  APEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASVVWSDGSHNVYVVYMGTSF 737
            APEGLTITVDPP LSFN IG K+SFTLTI GTISQSIVSASVVWSDGSHNVYVVYMGTSF
Sbjct: 1381 APEGLTITVDPPALSFNSIGDKKSFTLTIDGTISQSIVSASVVWSDGSHNVYVVYMGTSF 1440

Query: 738  EPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKLAEMDGVVSM 797
            EPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKLAEMDGVVSM
Sbjct: 1441 EPDYSTASKQHINMLQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKLAEMDGVVSM 1500

Query: 798  FVSEKKHLQTTRSWDFMGLFEGAPRTKLETDLIIGILDTGIWPESESFSDEGFDPPPAKW 857
            FVSEKKHLQTTRSWDFMGLFE APRT+LE++LIIGILDTGIWPESESFSDEGF PPPAKW
Sbjct: 1501 FVSEKKHLQTTRSWDFMGLFERAPRTRLESELIIGILDTGIWPESESFSDEGFGPPPAKW 1560

Query: 858  KGECQSSYNFTCNNKIIGARSFRSGATINCEHDNLSPRDTEGHGTLTSSTAAGNLVTGAS 917
            KGECQSSYNFTCNNKIIGARSFRSGA INCEHDNLSPRDTEGHGTLTSSTAAGNLVTGAS
Sbjct: 1561 KGECQSSYNFTCNNKIIGARSFRSGAIINCEHDNLSPRDTEGHGTLTSSTAAGNLVTGAS 1620

Query: 918  LFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHAIADGVDIISLSVAGSDLKD 977
            LFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHAIADGVDIISLSVAGSDLKD
Sbjct: 1621 LFGLGLGTSRGGSPSARIAVYKICWSEGCFDADILAAFDHAIADGVDIISLSVAGSDLKD 1680

Query: 978  YFEDSIAIGAFHAMKSGILTSNAGGNTGPLFGTIQNVSPWSLSVAASTTDRKFVTNVKLG 1037
            YFEDSIAIGAFHAMK+GILTSNAGGNTGPLFGTIQNVSPWSLSVAAST DRKFVTNVKLG
Sbjct: 1681 YFEDSIAIGAFHAMKNGILTSNAGGNTGPLFGTIQNVSPWSLSVAASTIDRKFVTNVKLG 1740

Query: 1038 NGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGFNGSSSRFCFNGSLDVNLIQGKIVLC 1097
            NGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGF+GSSSRFCFNGSLDVNLIQGKIVLC
Sbjct: 1741 NGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGFDGSSSRFCFNGSLDVNLIQGKIVLC 1800

Query: 1098 DGIADGEVSSGAVGMIMQDDTLQEAALLFPLPASQLDFNAGKNVFQYMRSTSNPVASIEK 1157
            DGIADG VSSGAVGMIMQDDTLQEAALLFPLP+SQLDFN GKNVFQYMRSTSNPVASIEK
Sbjct: 1801 DGIADGVVSSGAVGMIMQDDTLQEAALLFPLPSSQLDFNTGKNVFQYMRSTSNPVASIEK 1860

Query: 1158 STATKDLLAPYVVSFSSRGPNLLTPDILKPDLAAPGVDILASWPEGSAITGFTGDNRKAP 1217
            STATKDLLAPYVVSFSSRGP+LLTPDILKPDLAAPGVDILASWPEGSAITGFT DNRKAP
Sbjct: 1861 STATKDLLAPYVVSFSSRGPSLLTPDILKPDLAAPGVDILASWPEGSAITGFTWDNRKAP 1920

Query: 1218 FNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAEAEFAYGAGH 1277
            FNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAEAEFAYGAGH
Sbjct: 1921 FNIVSGTSIACPHATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAEAEFAYGAGH 1980

Query: 1278 LNPVNATNPGLVYDAQYIDYIEFLCGQGYSTENLRLVTGDQINCSDVRKTAASHLNYPSF 1337
            LNPVNATNPGLVYDAQYIDYI+FLCGQGYSTENLRLVTGDQINCSDVRKTAASHLNYPSF
Sbjct: 1981 LNPVNATNPGLVYDAQYIDYIKFLCGQGYSTENLRLVTGDQINCSDVRKTAASHLNYPSF 2040

Query: 1338 ALLIESQRLATCIYHRTVTNVGLSVSTYKAVIQAPSGLKVRVYPTTLSFSSLGQKISFTV 1397
            ALLI+SQRLATCIY RTVTNVGLSVSTYKAVIQAPSGLKVRVYPTTLSFSSLGQKISFTV
Sbjct: 2041 ALLIKSQRLATCIYRRTVTNVGLSVSTYKAVIQAPSGLKVRVYPTTLSFSSLGQKISFTV 2100

Query: 1398 RVEVESDEKMLSGSLTWDDGLHLVRSPIVAYHMPS 1433
            RVEVESDEKMLSGSLTWDDGLHLVRSPIVAYH+PS
Sbjct: 2101 RVEVESDEKMLSGSLTWDDGLHLVRSPIVAYHLPS 2135

BLAST of CmaCh04G001830 vs. NCBI nr
Match: KAE8648004.1 (hypothetical protein Csa_021411 [Cucumis sativus])

HSP 1 Score: 1758.0 bits (4552), Expect = 0.0e+00
Identity = 908/1396 (65.04%), Postives = 1077/1396 (77.15%), Query Frame = 0

Query: 51   HMRLLEEVVGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHT 110
            H  +L++V+ S+ +  +L++SY RSF+GFA RL ++EA+K+   D VVSVFP+ K  LHT
Sbjct: 16   HTSMLQQVLTSSDASKSLVYSYHRSFSGFAARLNDDEARKLAEMDEVVSVFPSEKHQLHT 75

Query: 111  TRSWDFMGFTTNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNN 170
            TRSWDFMGF     R   +ESD+++G+LD+GIWPES SFSD G+GP P+KWKG C+ S N
Sbjct: 76   TRSWDFMGFFQQASRTT-LESDLIIGMLDTGIWPESKSFSDEGFGPPPSKWKGECKPSLN 135

Query: 171  FRCNKKIIGARAYRSDNNFP-PEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTA 230
            F CN KIIGAR +RS    P   D  SPRD+ GHGTHT+ST  G  VS A+L+GLA GT+
Sbjct: 136  FTCNNKIIGARFFRSQPPSPGGADILSPRDTIGHGTHTSSTAGGNFVSDANLFGLAAGTS 195

Query: 231  RGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIG 290
            RGGVPSARIAVYKICW DGC+ ADILAAFD AIADGVDIIS+SVG   P+ YFNDSIAIG
Sbjct: 196  RGGVPSARIAVYKICWPDGCFGADILAAFDHAIADGVDIISISVGSIFPRNYFNDSIAIG 255

Query: 291  AFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYT 350
            AFH+MK+GILTSNS GN GP   +I N SPWSLSVAAS+IDRK V+ V LGN   F G +
Sbjct: 256  AFHAMKNGILTSNSGGNSGPSIGSISNVSPWSLSVAASTIDRKFVTKVTLGNGESFHGIS 315

Query: 351  INTFDLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTF 410
            +NTFD   K +PLI+AG APN + GF+GS SR C   S+D N V+GKI++CD ++S    
Sbjct: 316  LNTFDAGDKLFPLIHAGEAPNTTAGFNGSISRLCFPGSLDMNKVQGKIVLCD-LISDGEA 375

Query: 411  ASLSSAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVND 470
            A +S AVG +M  + + + A  +PLP S +   AG ++  Y+ SN+ P A I KS  + D
Sbjct: 376  ALISGAVGTIMQGSTLPEVAFLFPLPVSLINFNAGKNIFQYLRSNSNPEAIIEKSTTIED 435

Query: 471  TAAPIVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISG 530
             +AP V+SFSSRGPN  T DILKPDL A GV+ILA+W+   P++  + D R   +NIISG
Sbjct: 436  LSAPSVISFSSRGPNTVTLDILKPDLAASGVDILASWSEGTPITGIVGDKRIAPFNIISG 495

Query: 531  TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKA 590
            TSM+CPHAT AA YVK+FHPTWSPAAIKSALMT+A P++ +LN  AEFAYGAGH++P  A
Sbjct: 496  TSMACPHATGAAAYVKSFHPTWSPAAIKSALMTSAFPMSPKLNTDAEFAYGAGHLNPSNA 555

Query: 591  MNPGLIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFAL-STT 650
            +NPGL+YDA E DYV FLCGQGYST  +R ++GD + C+        DLNYPSF L   +
Sbjct: 556  INPGLVYDAEELDYVKFLCGQGYSTEKLRLVSGDQNNCSDVTKTAASDLNYPSFGLVIIS 615

Query: 651  PSESI-NQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTI- 710
            PS+ +  + + RT+TNVG  A      ++ AP GL +TV P  LSF  +G K SFT+T+ 
Sbjct: 616  PSQRLTTRVYHRTVTNVGLPA------VIKAPPGLKVTVRPATLSFRSLGQKISFTVTVR 675

Query: 711  -GGTISQSIVSASVVWSDGSH---NVYVVYMGTSFEPDYSTASKQHINMLQQVLTISDAS 770
                +   ++S S+ W DG H    VYVVYMG      +  AS+ H +MLQQVLT SDAS
Sbjct: 676  AKADVGGKVISGSLTWDDGVHLRKQVYVVYMGKPSGGGFLAASQLHTSMLQQVLTSSDAS 735

Query: 771  K-SLIYSYHRSFSGFAATLNDEEARKLAEMDGVVSMFVSEKKHLQTTRSWDFMGLFEGAP 830
            K SL+YSYHRSFSGFAA LN++EARKLA MDGVVS+F SEKK L TTRSWDFMG F+ AP
Sbjct: 736  KSSLVYSYHRSFSGFAARLNEDEARKLAVMDGVVSVFPSEKKQLHTTRSWDFMGFFQDAP 795

Query: 831  RTKLETDLIIGILDTGIWPESESFSDEGFDPPPAKWKGECQSSYNFTCNNKIIGARSFRS 890
             T+LE+D+IIG+LDTGIWPES+SFSDEGF PPP+KWKGEC+ + NFTCNNKIIGAR FRS
Sbjct: 796  TTRLESDIIIGMLDTGIWPESQSFSDEGFGPPPSKWKGECKPTLNFTCNNKIIGARFFRS 855

Query: 891  GATINCEHDNLSPRDTEGHGTLTSSTAAGNLVTGASLFGLGLGTSRGGSPSARIAVYKIC 950
               +    D  SPRD EGHGT TSSTA GN V+ A+LFGL  GTSRGG PSARIAVYKIC
Sbjct: 856  EPFVG--GDLPSPRDVEGHGTHTSSTAGGNFVSNANLFGLAAGTSRGGVPSARIAVYKIC 915

Query: 951  WSEGCFDADILAAFDHAIADGVDIISLSVAGSDLKDYFEDSIAIGAFHAMKSGILTSNAG 1010
            WS+GC DADILAAFDHAIADGVDIISLSV G    DY +D IAIGAFHAMK+GILTSN+G
Sbjct: 916  WSDGCPDADILAAFDHAIADGVDIISLSVGGFGASDYLDDPIAIGAFHAMKNGILTSNSG 975

Query: 1011 GNTGPLFGTIQNVSPWSLSVAASTTDRKFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIY 1070
            GN GP  G+I NVSPWSLSVAAST DRKFVTNV LGNG+S  GISVNTF LGDKLFPLI+
Sbjct: 976  GNDGPNLGSISNVSPWSLSVAASTIDRKFVTNVALGNGESIQGISVNTFDLGDKLFPLIH 1035

Query: 1071 AGDAPNTTAGFNGSSSRFCFNGSLDVNLIQGKIVLCDGIADGEV--SSGAVGMIMQDDTL 1130
            AGDAPNTTAGFNGS+SR CF GSLD + +QGKIV+CD I+DGEV  SSGAVG IMQ+   
Sbjct: 1036 AGDAPNTTAGFNGSTSRLCFPGSLDEDKVQGKIVICDLISDGEVTQSSGAVGTIMQNPNF 1095

Query: 1131 QEAALLFPLPASQLDFNAGKNVFQYMRSTSNPVASIEKSTATKDLLAPYVVSFSSRGPNL 1190
            Q+ A LFP P S + FN G+ +FQY+RS SNP A+IEKST  +DL AP VVSFSSRGPNL
Sbjct: 1096 QDVAFLFPQPVSLISFNTGEKLFQYLRSNSNPEAAIEKSTTIEDLSAPAVVSFSSRGPNL 1155

Query: 1191 LTPDILKPDLAAPGVDILASWPEGSAITGFTGDNRKAPFNIVSGTSIACPHATGAAAYVK 1250
            +T DILKPDLAAPGVDILASW EG++ITG  GD R APFNI+SGTS+ACPHATGAAAYVK
Sbjct: 1156 ITLDILKPDLAAPGVDILASWSEGTSITGLVGDKRIAPFNIISGTSMACPHATGAAAYVK 1215

Query: 1251 SFHPTWSPAAIKSALMTTALPMTPKVNAEAEFAYGAGHLNPVNATNPGLVYDAQYIDYIE 1310
            SFHPTWSPAAIKSALMT+A PM+PK+N +AE  YGAGHLNP NA NPGLVYDA+ +DYI+
Sbjct: 1216 SFHPTWSPAAIKSALMTSAFPMSPKLNTDAELGYGAGHLNPSNAINPGLVYDAEELDYIK 1275

Query: 1311 FLCGQGYSTENLRLVTGDQINCSDVRKTAASHLNYPSFALLIE--SQRLATCIYHRTVTN 1370
            FLCGQGYST++LRLV+GD  NCSDV KTAAS LNYPSF L+I   SQRL + +YHRTVTN
Sbjct: 1276 FLCGQGYSTKDLRLVSGDHSNCSDVTKTAASDLNYPSFGLVINSTSQRLISRVYHRTVTN 1335

Query: 1371 VGLSVSTYKAVIQAPSGLKVRVYPTTLSFSSLGQKISFTVRVEVESD--EKMLSGSLTWD 1430
            VGL VSTYKAVI+AP GLKV V P TLSF SLGQKISFTV V  +++   K++SGSLTWD
Sbjct: 1336 VGLPVSTYKAVIKAPPGLKVTVRPATLSFRSLGQKISFTVTVRAKANVVGKVVSGSLTWD 1395

Query: 1431 DGLHLVRSPIVAYHMP 1432
            DG+HLVRSPIV++ +P
Sbjct: 1396 DGVHLVRSPIVSFVLP 1401

BLAST of CmaCh04G001830 vs. NCBI nr
Match: KAF8397395.1 (hypothetical protein HHK36_016308 [Tetracentron sinense])

HSP 1 Score: 1729.5 bits (4478), Expect = 0.0e+00
Identity = 891/1399 (63.69%), Postives = 1059/1399 (75.70%), Query Frame = 0

Query: 32   VYIVYLGNKPDDMASTPSHHMRLLEEVVGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKI 91
            VY+VY+G++P    S  S HM +LE VVGS  S ++LLHSYKRSFNGF  RLT+EE QK+
Sbjct: 758  VYVVYMGDRPKGDFSASSLHMGMLEAVVGSGAS-ESLLHSYKRSFNGFVARLTKEEMQKL 817

Query: 92   RAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVGVLDSGIWPESPSFSD 151
               +GVVSVFP+ KK LHTTRSWDFMGF     R  +VESDIVVGVLD+GIWPES SFSD
Sbjct: 818  AGMEGVVSVFPSEKKKLHTTRSWDFMGFPQQAKR-TEVESDIVVGVLDTGIWPESASFSD 877

Query: 152  VGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDFESPRDSDGHGTHTASTV 211
             G+GP P+KWKG CQAS+NF CN KIIGAR Y SD      DF SPRDS+GHGTHT+ST 
Sbjct: 878  EGFGPPPSKWKGTCQASSNFTCNNKIIGARFYHSDGQVGVADFASPRDSEGHGTHTSSTA 937

Query: 212  AGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISL 271
            AG LVS+ASL GL LGT+RGGVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISL
Sbjct: 938  AGRLVSKASLLGLGLGTSRGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISL 997

Query: 272  SVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDR 331
            SVGG  P  YF+DSIAIGAFHSMK+GILTSNSAGN GP   +I NFSPWSLSVAAS+IDR
Sbjct: 998  SVGGFFPLDYFDDSIAIGAFHSMKNGILTSNSAGNSGPSAASISNFSPWSLSVAASTIDR 1057

Query: 332  KLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRN 391
            K V+ VQLGN  +F+G +INTF+L+   YP+IY G+APN S GF GS SR+C + S+D  
Sbjct: 1058 KFVTKVQLGNSMVFEGISINTFELKNDTYPMIYGGNAPNTSQGFDGSESRFCGQGSLDTT 1117

Query: 392  LVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYI 451
            LVKGKI++CD  L       L+ A+G VM   G +D A S+PL ++YL    G+ V +Y+
Sbjct: 1118 LVKGKIVLCDQ-LDTGEGPLLAGAIGTVMQGGGFRDVAFSFPLSATYLDVKDGSAVSDYL 1177

Query: 452  GSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAP 511
             S +  T  I KSNE  D +AP+VVSFSSRGPNP T DILKPDLTAPGV+ILA+W+P++ 
Sbjct: 1178 NSTSNATGIILKSNESKDVSAPVVVSFSSRGPNPITSDILKPDLTAPGVDILASWSPVSS 1237

Query: 512  VSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVEL 571
            VS    D+R   +NIISGTSMSCPHAT AA Y+K+FHPTWSPAAIKSALMTTA  ++V  
Sbjct: 1238 VSGVAGDNRLEPFNIISGTSMSCPHATGAAAYIKSFHPTWSPAAIKSALMTTAFTMSVAS 1297

Query: 572  NPQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAAN 631
            N  AEFAYGAGHIDP+KA NPGL+YDA E DYV FLCGQGYS+  ++ +TGD S CT A 
Sbjct: 1298 NIDAEFAYGAGHIDPIKAANPGLVYDAGEVDYVKFLCGQGYSSKSLQLVTGDNSTCTTAT 1357

Query: 632  SGRVWDLNYPSFALSTTPSESINQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVL 691
            +G VWDLNYPSFAL T    S N+ F RT+TNVGS  STYT+ +  AP  L I V P VL
Sbjct: 1358 NGTVWDLNYPSFALFTPTEVSFNRSFHRTVTNVGSPVSTYTANVT-APSSLKIQVVPSVL 1417

Query: 692  SFNGIGHKESFTLTIGGTISQSIVSASVVWSDGSHNVYVVYMGTSFEPDYSTASKQHINM 751
            SF  +G ++SF +T+ G+IS +IVSAS+VW+DG   VYVVYMG   + D+S AS  H+ M
Sbjct: 1418 SFKSLGQRQSFVVTVEGSISSTIVSASLVWNDGVFQVYVVYMGDRPKGDFS-ASSLHMGM 1477

Query: 752  LQQVLTISDASKSLIYSYHRSFSGFAATLNDEEARKLAEMDGVVSMFVSEKKHLQTTRSW 811
            L+ V+  S AS+SL++SY RSF+GF A L  EE +KLA M+GVVS+F SEKK L TTRSW
Sbjct: 1478 LEAVVG-SGASESLLHSYKRSFNGFVARLTKEEMQKLAGMEGVVSVFPSEKKKLHTTRSW 1537

Query: 812  DFMGLFEGAPRTKLETDLIIGILDTGIWPESESFSDEGFDPPPAKWKGECQSSYNFTCNN 871
            DFMG  + A RT++E+D+++G+LDTGIWPES SFSDEGF PPP+KWKG CQ+S NFTCNN
Sbjct: 1538 DFMGFPQQAKRTEVESDIVVGVLDTGIWPESASFSDEGFGPPPSKWKGTCQASSNFTCNN 1597

Query: 872  KIIGARSFRSGATINCEHDNLSPRDTEGHGTLTSSTAAGNLVTGASLFGLGLGTSRGGSP 931
            KIIGAR + S   +    D  SPRD+EGHGT TSSTAAG LV+ ASL GLGLGTSRGG P
Sbjct: 1598 KIIGARFYHSDGQVGV-GDFASPRDSEGHGTHTSSTAAGRLVSKASLLGLGLGTSRGGVP 1657

Query: 932  SARIAVYKICWSEGCFDADILAAFDHAIADGVDIISLSVAGSDLKDYFEDSIAIGAFHAM 991
            SARIAVYKICWS+GC DADILAAFD AIADGVDIISLSV G    DYF+DSIAIGAFH+M
Sbjct: 1658 SARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGFFPLDYFDDSIAIGAFHSM 1717

Query: 992  KSGILTSNAGGNTGPLFGTIQNVSPWSLSVAASTTDRKFVTNVKLGNGDSFLGISVNTFQ 1051
            K+GILTSN+ GN+GP   +I N SPWSLSVAAST DRKFVT V+LGN   F GIS+NTF+
Sbjct: 1718 KNGILTSNSAGNSGPSAASISNFSPWSLSVAASTIDRKFVTKVQLGNSMVFEGISINTFE 1777

Query: 1052 LGDKLFPLIYAGDAPNTTAGFNGSSSRFCFNGSLDVNLIQGKIVLCDGIADGE--VSSGA 1111
            L +  +P+IY G+APNT+ GF+GS SRFC  GSLD  L++GKIVLCD +  GE  + +GA
Sbjct: 1778 LKNDTYPMIYGGNAPNTSQGFDGSESRFCGQGSLDTTLVKGKIVLCDQLDTGEGPLLAGA 1837

Query: 1112 VGMIMQDDTLQEAALLFPLPASQLDFNAGKNVFQYMRSTSNPVASIEKSTATKDLLAPYV 1171
            +G +MQ    ++ A  FPL A+ LD   G  V  Y+ STSN    I KS   KD+ AP V
Sbjct: 1838 IGTVMQGGGFRDVAFSFPLSATYLDVKDGSAVSDYLNSTSNATGIILKSNEAKDVSAPVV 1897

Query: 1172 VSFSSRGPNLLTPDILKPDLAAPGVDILASWPEGSAITGFTGDNRKAPFNIVSGTSIACP 1231
            VSFSSRGPN +T DILKPDL APGVDILASW   S+++G  GDNR   FNI+SGTS++CP
Sbjct: 1898 VSFSSRGPNPITSDILKPDLTAPGVDILASWSPVSSVSGVDGDNRLESFNIISGTSMSCP 1957

Query: 1232 HATGAAAYVKSFHPTWSPAAIKSALMTTALPMTPKVNAEAEFAYGAGHLNPVNATNPGLV 1291
            HATGAAAY+KSFH TWSPAAIKSALMTTA  M+   N +AEFAYGAGH++P+ A NPGLV
Sbjct: 1958 HATGAAAYIKSFHTTWSPAAIKSALMTTASTMSVVSNIDAEFAYGAGHIDPIKAANPGLV 2017

Query: 1292 YDAQYIDYIEFLCGQGYSTENLRLVTGDQINCSDVRKTAASHLNYPSFALLIESQRLATC 1351
            YDA  +DY++FLCGQGYS+++L+LVTGD   C+         LNYPSFAL   ++     
Sbjct: 2018 YDAGEVDYVKFLCGQGYSSKSLQLVTGDNSTCTTATNGTVWDLNYPSFALFTPTEVSFNR 2077

Query: 1352 IYHRTVTNVGLSVSTYKAVIQAPSGLKVRVYPTTLSFSSLGQKISFTVRVEVESDEKMLS 1411
             +HRTVTNVG  VSTY A + APS LK++V P+ LSF SLGQ+ SF V VE      ++S
Sbjct: 2078 SFHRTVTNVGSPVSTYTANVTAPSSLKIQVVPSVLSFKSLGQRQSFVVTVEGSISSTIVS 2137

Query: 1412 GSLTWDDGLHLVRSPIVAY 1429
             SL W+DG+  VRSPIV +
Sbjct: 2138 ASLVWNDGVFQVRSPIVVH 2149

BLAST of CmaCh04G001830 vs. NCBI nr
Match: KAA3481900.1 (cucumisin-like [Gossypium australe])

HSP 1 Score: 1645.6 bits (4260), Expect = 0.0e+00
Identity = 843/1438 (58.62%), Postives = 1033/1438 (71.84%), Query Frame = 0

Query: 9    LVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGSTF-SPDA 68
            L  + F   +L       +D R+VYIVY+GN P    S  S H  +L++V+ S     D 
Sbjct: 11   LSIISFTLAMLICCCHGSSDNRQVYIVYMGNLPKGEVSISSLHTSMLQQVLPSNIVGSDV 70

Query: 69   LLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNVPRVN 128
            LL+SY RSFNGFA +LT++EA+K++ KDGVVSVF + K+ LHT+RSWDFMGF   V R +
Sbjct: 71   LLYSYHRSFNGFAAKLTKDEAEKLKEKDGVVSVFLSQKRQLHTSRSWDFMGFNRKVKR-S 130

Query: 129  QVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDN 188
              ESDI++G+LD+GIWPES SF+D  +G +PAKWKG CQ S NF CN+KIIGA+ Y++D 
Sbjct: 131  VFESDIIIGMLDTGIWPESQSFNDTEFGAIPAKWKGTCQTSANFTCNRKIIGAKYYKADG 190

Query: 189  NFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSD 248
            +F P +++SPRDS+GHG+HT+ST AGGLVS+ASLYGLA GTARGGVPSARIAVYKICWS 
Sbjct: 191  DFDPSEYQSPRDSEGHGSHTSSTAAGGLVSKASLYGLAKGTARGGVPSARIAVYKICWSI 250

Query: 249  GCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGND 308
            GC D DILAAFDDAIADGVDIISLSVG   P  YF+D+IAIGAFHSMK+GILTSNSAGN 
Sbjct: 251  GCDDVDILAAFDDAIADGVDIISLSVGSIFPSEYFDDTIAIGAFHSMKNGILTSNSAGNS 310

Query: 309  GPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGH 368
            GP   TI NFSPWSLSVAAS+IDRK V+ V+LGN  I++G +INTFDL+GK YP I    
Sbjct: 311  GPSLSTITNFSPWSLSVAASTIDRKFVTRVKLGNGEIYEGTSINTFDLKGKMYPFIAGAA 370

Query: 369  APNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFAS---LSSAVGVVMNNAG 428
            APN S G++   SR+C   ++D  LVKGKI+ CD     +T+      + AVGVV+ +  
Sbjct: 371  APNTSQGYTSDDSRFCGPGTLDETLVKGKIVFCDY----TTYIEGPVEAGAVGVVVQSGR 430

Query: 429  VKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPN 488
             KD A +Y LP S L    G +V NY+     PTATIFK+   +D  AP VV+FSSRGPN
Sbjct: 431  YKDYAFTYGLPLSNLNLNVGRNVLNYVNLTENPTATIFKTTVEDDQFAPFVVTFSSRGPN 490

Query: 489  PETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYV 548
            P T DILKPD+TAPGV+ILAAW+   P+S    D+R   YNIISGTSMSCPHAT AA YV
Sbjct: 491  PATPDILKPDVTAPGVDILAAWSEAVPLSDTEDDTRIVPYNIISGTSMSCPHATGAAAYV 550

Query: 549  KTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANETDYV 608
            K+FHPTWSPAAIKSALMTTA P++ E N +AEFAYGAGHI+P +A  PGL+YDA E DYV
Sbjct: 551  KSFHPTWSPAAIKSALMTTAFPMSSENNLEAEFAYGAGHINPAQAAQPGLVYDAGEIDYV 610

Query: 609  NFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNV 668
             FLCGQGY+   ++ +T     C+   +G VWDLNYPSF LST+P  SI + F RT+ NV
Sbjct: 611  KFLCGQGYTPKQLKLITESNFTCSEETNGTVWDLNYPSFTLSTSPGNSITRVFHRTVMNV 670

Query: 669  GSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASVVWSDG 728
            GS  STY + ++ AP GL I V P VLSF  +G K +F +T+G  I  S++S S++W DG
Sbjct: 671  GSPVSTYKA-VVNAPPGLIIQVQPSVLSFKSLGQKLTFVVTVGAEIGNSMISGSLIWDDG 730

Query: 729  SHN-----------VYVVYMGTSFEPDYSTASKQHINMLQQVLTISD-ASKSLIYSYHRS 788
             H            VY+VYMG   +   ST S  H +ML +VL  S   S+ L+YSYHRS
Sbjct: 731  VHQVRSPIVAYASLVYIVYMGNLPKGKVSTTS-LHTSMLHEVLPSSTVGSEVLLYSYHRS 790

Query: 789  FSGFAATLNDEEARKLAEMDGVVSMFVSEKKHLQTTRSWDFMGLFEGAPRTKLETDLIIG 848
            F+GFAA L  +EA KL E +GVVS+F S+KK L TTRSWDFMG  +   R+ +E+D+I+G
Sbjct: 791  FNGFAAKLTKDEAAKLREKEGVVSVFRSQKKQLHTTRSWDFMGFNKKVKRSIIESDIIVG 850

Query: 849  ILDTGIWPESESFSDEGFDPPPAKWKGECQSSYNFTCNNKIIGARSFRSGATINCEHDNL 908
            +LDTGIWPES+SF D G  P P KWKG CQSS NFTCN KIIGA+ +R+   I   +D  
Sbjct: 851  MLDTGIWPESQSFDDTGLGPIPQKWKGTCQSSANFTCNRKIIGAKYYRAKGDI--PYDYK 910

Query: 909  SPRDTEGHGTLTSSTAAGNLVTGASLFGLGLGTSRGGSPSARIAVYKICWSEGCFDADIL 968
            +PRD EGHGT TSSTAAG LV+ ASL+G+  GT+RGG PSARIAVYKICWS+GC+D DIL
Sbjct: 911  TPRDYEGHGTHTSSTAAGGLVSKASLYGIAKGTARGGVPSARIAVYKICWSDGCYDEDIL 970

Query: 969  AAFDHAIADGVDIISLSVAGSDLKDYFEDSIAIGAFHAMKSGILTSNAGGNTGPLFGTIQ 1028
            AAFD AIADGVDIISLSV      +YF+D+IAIGAFH+MK+G+LTSN+ GN+GP+  +I 
Sbjct: 971  AAFDDAIADGVDIISLSVGSFFASEYFDDTIAIGAFHSMKNGVLTSNSAGNSGPILSSIT 1030

Query: 1029 NVSPWSLSVAASTTDRKFVTNVKLGNGDSFLGISVNTFQLGDKLFPLIYAGDAPNTTAGF 1088
            N SPWSLSVAAST DRKF+T VKLGNG  + G S+NTF L  K++P I    APNT+ G+
Sbjct: 1031 NFSPWSLSVAASTIDRKFITKVKLGNGKIYEGTSINTFDLKGKMYPFIAGAAAPNTSQGY 1090

Query: 1089 NGSSSRFCFNGSLDVNLIQGKIVLCDGIAD--GEVSSGAVGMIMQDDTLQEAALLFPLPA 1148
            +   SRFC  G+L+  L++GKIV CD  +D  G   +GA+G + Q    ++    + LP 
Sbjct: 1091 SPEGSRFCGPGTLNETLVKGKIVFCDYDSDGTGPAEAGAIGAVFQSGIYKDYVFPYSLPL 1150

Query: 1149 SQLDFNAGKNVFQYMRSTSNPVASIEKSTATKDLLAPYVVSFSSRGPNLLTPDILKPDLA 1208
            S L+ + G+NV  Y+ +T  P A+I K+    D  AP+VVSFSSRGPN  TPDILKPD+ 
Sbjct: 1151 SNLNLDDGRNVLNYVNTTEKPTATIFKTNVVDDQFAPFVVSFSSRGPNPATPDILKPDVT 1210

Query: 1209 APGVDILASWPEGSAITGFTGDNRKAPFNIVSGTSIACPHATGAAAYVKSFHPTWSPAAI 1268
            APGVDILA+W E   +T    D R  P+NI+SGTS++CPHATGAAAYVKSFHPTWSPAAI
Sbjct: 1211 APGVDILAAWSEAVPLTETKDDTRIVPYNIISGTSMSCPHATGAAAYVKSFHPTWSPAAI 1270

Query: 1269 KSALMTTALPMTPKVNAEAEFAYGAGHLNPVNATNPGLVYDAQYIDYIEFLCGQGYSTEN 1328
            KSALMTTA PM+ K N EAEFAYGAGH+NP  A  PGLVYDA  IDY++FLCGQGY+ + 
Sbjct: 1271 KSALMTTAFPMSSKNNLEAEFAYGAGHINPAQAAQPGLVYDAGEIDYVKFLCGQGYTPKQ 1330

Query: 1329 LRLVTGDQINCSDVRKTAASHLNYPSFALLIESQRLATCIYHRTVTNVGLSVSTYKAVIQ 1388
            L L+T     CS+    A   LNYPSF L        T ++HRTVTNVG  VSTYK V+ 
Sbjct: 1331 LNLITESNFTCSEETNGAVWDLNYPSFTLSTTPGNSITRVFHRTVTNVGSPVSTYKVVVN 1390

Query: 1389 APSGLKVRVYPTTLSFSSLGQKISFTVRVEVESDEKMLSGSLTWDDGLHLVRSPIVAY 1429
            AP GL ++V P+ LSF SLGQK++F V V  E    M+SGSL WDDG+H VRSPIVAY
Sbjct: 1391 APPGLIIQVQPSVLSFKSLGQKLTFIVTVGAEIGNSMISGSLIWDDGVHQVRSPIVAY 1439

BLAST of CmaCh04G001830 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 646.0 bits (1665), Expect = 7.3e-185
Identity = 338/707 (47.81%), Postives = 460/707 (65.06%), Query Frame = 0

Query: 37  LGNKPDDMASTPSHHMRLLEEVVGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDG 96
           +G  P+   S PSHH+ +L+++VG+  +   L+ SYKRSFNGFA  L++ E+QK++    
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 97  VVSVFPNGKKHLHTTRSWDFMGFTTNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGP 156
           VVSVFP+    L TTRSWDF+GF     R +  ESD++VGV+DSGIWPES SF D G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 157 LPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLV 216
            P KWKG+C+    F CN K+IGAR Y   N F     +S RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFY---NKFA----DSARDEEGHGTHTASTAAGNAV 180

Query: 217 SQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 276
             AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 277 EPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSD 336
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW ++VAAS  DR+ +  
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 337 VQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGK 396
           V LGN     G ++NTF+L G  +P++Y     N+S   S + + YCS   VD  LVKGK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 397 ILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNF 456
           I++CD  L     A L+ A+GV++ N  + D+A   P P+S L       +K+YI S   
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 457 PTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGI 516
           P A I ++ E+ D  AP V SFSSRGP+    ++LKPD++APG+EILAA++P+A  SS +
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 517 --KDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQ 576
             +D R+  Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTA P+N++ NP+
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 577 AEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGR 636
            EFAYG+G I+P KA +PGL+Y+    DY+  LC +G+ +  +   +G    C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 637 VWDLNYPSFALSTTPSESINQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFN 696
           V DLNYP+     +  +  N  F RT+TNVG   STY + ++     L I+++P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 697 GIGHKESFTLTIGGTISQ--SIVSASVVWSDGSHNVYVVYMGTSFEP 740
            +  K+SF +TI G   +  S VS+SVVWSDGSH+V    +  S +P
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSIQP 693

BLAST of CmaCh04G001830 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 625.2 bits (1611), Expect = 1.3e-178
Identity = 341/737 (46.27%), Postives = 465/737 (63.09%), Query Frame = 0

Query: 4   SVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTP-SHHMRLLEEVVGST 63
           S IF L+ + F      +S   D+  ++VYIVY+G  P  +   P SHH  +L++V G +
Sbjct: 11  SCIFALLVVSF------ASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGES 70

Query: 64  FSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFT-- 123
              D L+ +YKRSFNGFA RLTE E + + + D VVSVFP+   +L TT SW+FMG    
Sbjct: 71  SIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEG 130

Query: 124 TNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGA 183
               R   +ESD ++GV+DSGI+PES SFS  G+GP P KWKG C+   NF CN K+IGA
Sbjct: 131 KRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGA 190

Query: 184 RAYRSD-NNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIA 243
           R Y      FP    ES RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARIA
Sbjct: 191 RYYTPKLEGFP----ESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIA 250

Query: 244 VYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHG 303
           VYK+C      C    ILAAFDDAIAD VDII++S+G      +  D++AIGAFH+M  G
Sbjct: 251 VYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKG 310

Query: 304 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQG 363
           ILT N AGN+GP+  TI + +PW  +VAAS+++R  ++ V LGN     G ++N+FDL G
Sbjct: 311 ILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNG 370

Query: 364 KHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVG 423
           K YPL+Y   A   S     SS+ +CS   +D   VKGKI++CD+  +P    ++ +   
Sbjct: 371 KKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVAS 430

Query: 424 VVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVS 483
           +V N    +D A  +  P S L+    N V +Y+ S   P A + KS  + +  AP+V S
Sbjct: 431 IVRN--PYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVAS 490

Query: 484 FSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHA 543
           +SSRGPNP  +DILKPD+TAPG EILAA++P  P S    D+R   Y +ISGTSMSCPH 
Sbjct: 491 YSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHV 550

Query: 544 TAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQ---AEFAYGAGHIDPLKAMNPGL 603
              A Y+KTFHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH+DP+ A++PGL
Sbjct: 551 AGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGL 610

Query: 604 IYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVW-DLNYPSFALSTTPSESI 663
           +Y+AN++D++ FLCG  Y+   +R ++GD S CT   +  +  +LNYPS +   + ++  
Sbjct: 611 VYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPF 670

Query: 664 NQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGT--IS 723
              F RT+TNVG   +TY +K++G+   L + V P VLS   +  K+SFT+T+ G    +
Sbjct: 671 KVTFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKA 728

Query: 724 QSIVSASVVWSDGSHNV 729
           +++VSA ++WSDG H V
Sbjct: 731 ENLVSAQLIWSDGVHFV 728

BLAST of CmaCh04G001830 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 620.2 bits (1598), Expect = 4.3e-177
Identity = 333/733 (45.43%), Postives = 467/733 (63.71%), Query Frame = 0

Query: 11  FLGFFGCLLASSLDS------DNDGRKVYIVYLGNKPDDMASTP-SHHMRLLEEVVGSTF 70
           F+  F  LL  SL S      D+  ++VYIVYLG+ P     TP S HM +L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 71  SPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNV 130
             + L+ SYK+SFNGFA RLTE E +++   + VVSVFP+ K  L TT SW+FMG    +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 131 --PRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGAR 190
              R   +ESD ++GV+DSGI+PES SFSD G+GP P KWKG C    NF CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 191 AYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVY 250
            Y + +    +  ++ RD  GHGTHTAS  AG  V+ ++ YGL  GTARGGVP+ARIAVY
Sbjct: 187 DYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 251 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTS 310
           K+C ++GC    +++AFDDAIADGVD+IS+S+       +  D IAIGAFH+M  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 311 NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYP 370
           N+AGN+GP   T+ + +PW  SVAAS  +R  ++ V LG+  I  G ++NT+D+ G +YP
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 371 LIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMN 430
           L+Y   A       S   +R C    +D  LVKGKI++CDS         L +   +V N
Sbjct: 367 LVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKN 426

Query: 431 NAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSR 490
               +   RS+P+  S+L+      + +Y+ S   P AT+ KS E+++  AP+V SFSSR
Sbjct: 427 PEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSR 486

Query: 491 GPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAA 550
           GP+    DILKPD+TAPGVEILAA++P +  +    D+R   Y+++SGTSM+CPH    A
Sbjct: 487 GPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVA 546

Query: 551 VYVKTFHPTWSPAAIKSALMTTAIPLNVELN--PQAEFAYGAGHIDPLKAMNPGLIYDAN 610
            YVKTFHP WSP+ I+SA+MTTA P+N   +     EFAYG+GH+DP+ A+NPGL+Y+  
Sbjct: 547 AYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELT 606

Query: 611 ETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRV-WDLNYPSFALSTTPSESINQFFT 670
           + D++NFLCG  Y++  +R ++GD S CT   S  +  +LNYP+ +   + ++  N  F 
Sbjct: 607 KADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQ 666

Query: 671 RTLTNVGSEASTYTSKILGAP-EGLTITVDPPVLSFNGIGHKESFTLTIGGTI--SQSIV 729
           RT+TNVG + STY +K++  P   L+I V P VLS   +  K+SF +T+      ++  V
Sbjct: 667 RTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPV 726

BLAST of CmaCh04G001830 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 619.0 bits (1595), Expect = 9.5e-177
Identity = 335/720 (46.53%), Postives = 453/720 (62.92%), Query Frame = 0

Query: 18  LLASSLDSDNDGRKVYIVYLGNKPDDMASTP-SHHMRLLEEVVGSTFSPDALLHSYKRSF 77
           L  SS+ +  D ++VYIVY+G+       TP S HM +L+EV G +     L+ SYKRSF
Sbjct: 17  LFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSF 76

Query: 78  NGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTNV--PRVNQVESDIV 137
           NGFA RLTE E +++    GVVSVFPN K  L TT SWDFMG    +   R   VESD +
Sbjct: 77  NGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTI 136

Query: 138 VGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARAYRSDNNFPPEDF 197
           +GV+DSGI PES SFSD G+GP P KWKG C    NF CN K+IGAR Y S         
Sbjct: 137 IGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS--------- 196

Query: 198 ESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADI 257
           E  RD DGHGTHTAST AG  V  AS +G+  GT RGGVP++R+A YK+C   GC    +
Sbjct: 197 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 256

Query: 258 LAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTI 317
           L+AFDDAIADGVD+I++S+G      + ND IAIGAFH+M  G+LT NSAGN GP   ++
Sbjct: 257 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 316

Query: 318 RNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPLIYAGHAPNISGG 377
              +PW L+VAAS+ +R  V+ V LGN     G ++N ++++GK YPL+Y   A   S  
Sbjct: 317 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSA--ASSA 376

Query: 378 FSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMNNAGVKDNARSYPL 437
               S+  C  + VD++ VKGKILVC          S+  AVG++       D A  +PL
Sbjct: 377 CDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLIYRTP-KPDVAFIHPL 436

Query: 438 PSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRGPNPETYDILKPD 497
           P++ L       + +Y+ S + P A + K+  + +  +P++ SFSSRGPN    DILKPD
Sbjct: 437 PAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPD 496

Query: 498 LTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPA 557
           +TAPGVEILAA++P    S    D+R   Y+++SGTSMSCPH    A YVKTF+P WSP+
Sbjct: 497 ITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPS 556

Query: 558 AIKSALMTTAIPLNVELN--PQAEFAYGAGHIDPLKAMNPGLIYDANETDYVNFLCGQGY 617
            I+SA+MTTA P+N         EFAYG+GH+DP+ A NPGL+Y+ +++D++ FLCG  Y
Sbjct: 557 MIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNY 616

Query: 618 STAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPS-ESINQFFTRTLTNVGSEASTY 677
           ++ +++ ++G+   C+ A      +LNYPS +   + S  +    F RTLTNVG+  STY
Sbjct: 617 TSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTY 676

Query: 678 TSKIL-GAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIV--SASVVWSDGSHNV 729
           TSK++ G    L + + P VLSF  +  K+SFT+T+ G+   S V  SA+++WSDG+HNV
Sbjct: 677 TSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNV 721

BLAST of CmaCh04G001830 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 615.1 bits (1585), Expect = 1.4e-175
Identity = 342/737 (46.40%), Postives = 463/737 (62.82%), Query Frame = 0

Query: 4   SVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTP-SHHMRLLEEVVGST 63
           S IF L+ + F      +S D D+  ++ YIVY+G  P  +   P SHH  +L++V G +
Sbjct: 12  SCIFALLVVSF------ASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES 71

Query: 64  FSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGF--T 123
              D L+ +YKRSFNGFA RLT+ E + + + D VVSVFPN K  L TT SW+FMG   +
Sbjct: 72  SIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKES 131

Query: 124 TNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGA 183
               R   +ESD ++GV+DSGI+PES SFS  G+GP P KWKG C+   NF  N K+IGA
Sbjct: 132 KRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGA 191

Query: 184 RAYRSD-NNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIA 243
           R Y      FP    ES RD  GHG+HTAST AG  V   S YGL  GTARGGVP+ARIA
Sbjct: 192 RYYTPKLEGFP----ESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIA 251

Query: 244 VYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHG 303
           VYK+C    DGC    ILAAFDDAIAD VDII++S+GG     +  D IAIGAFH+M  G
Sbjct: 252 VYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKG 311

Query: 304 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQG 363
           IL  NSAGN GP+  T+ + +PW  +VAAS+ +R  V+ V LGN     G ++N+FDL G
Sbjct: 312 ILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNG 371

Query: 364 KHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVG 423
           K YPL+Y   A +  G    +S+ +CS   +D   VKGKI++CDS  +P    ++ +   
Sbjct: 372 KKYPLVYGKSASSSCG---AASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIAS 431

Query: 424 VVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVS 483
           +V ++    D A  +  P S L     N V +Y+ S   P A + KS  + +  AP+V S
Sbjct: 432 IVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVAS 491

Query: 484 FSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHA 543
           + SRGPN    DILKPD+TAPG EI+AA++P AP S  I D+R   Y++ +GTSMSCPH 
Sbjct: 492 YFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHV 551

Query: 544 TAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNP---QAEFAYGAGHIDPLKAMNPGL 603
              A Y+K+FHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH+DP+ A++PGL
Sbjct: 552 AGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGL 611

Query: 604 IYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRV-WDLNYPSFALSTTPSESI 663
           +Y+AN++D++ FLCG  Y+   +R ++GD S CT   +  +  +LNYPS     + ++  
Sbjct: 612 VYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPF 671

Query: 664 NQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGT--IS 723
              F RT+TNVG   +TY +K++G+   L + V P VLS   +  K+SFT+T  G    +
Sbjct: 672 KVIFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTASGAGPKA 728

Query: 724 QSIVSASVVWSDGSHNV 729
           +++VSA ++WSDG H V
Sbjct: 732 ENLVSAQLIWSDGVHFV 728