CmaCh04G000870 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G000870
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionheat stress transcription factor A-8
LocationCma_Chr04: 434488 .. 440081 (+)
RNA-Seq ExpressionCmaCh04G000870
SyntenyCmaCh04G000870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAGCGTCCACTGACATGCCTGCCTCTCAATAACCCAACCACACGACGCGGCGAAAAAGGGATAAACCCCAAAATTCTTCCTGTTCTACCCAATTACCCGAATCATTTTTCTATGCTTTCCTTCTAATTTCTTCCTTTATCCTTTTGCTTTCCTCCTAATTACGCTATCTAATCTCATCGGACTTCGAGAATTTTGCGATGGTGAAATCCGCGGGGGAGCGGCAGGGATCTTCGGTGGCGCCTTTTCTGAAGAAATTGTACGAGATGGTCGACGATGAGGCAACCAATTCCATTATCTCGTGGAGTCCGAGGGATGATAGCTTTACGATTCTGGACATGACTCATTTCTCTGTCCACTTGCTTCCCAAGTACTTTAAGCACAGTAATTTCTCTAGCTTTATGCGGCAGCTCAACATCTATGTGAGTTTCTTGTTTGTTTTATTGTCGTTTGAAATTTTCGATGCGATGTTTGTGTTCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGCCTTGCTGATTTTTCTTCTCTGGTATTCATTCTAATAGACTTGCGATATATCTTATCTTGTTTATGCAGTCGTCATTTGTTGTTCTTGAGTTCTGATTAAATGGGAAAGTTGCTCTGTCGTGGACTCATCTTCTTTCTAGTTGCCCTAATTGGTAATAGCGCAATTGAGGATTATAGATTTCACAAACTGGGAGGAGTTATATTCAAGATTGACTTGGAAAAGGCTTCGATGGGGACTTGATTGAAATTATGTTAGGTCGCTTAATTACTCTGTTTTGGTTAATGGTAAGCTCAGAGGAGAATTTTGCCTCCACACAGGGGGAACCTTAGCCCTTTCTTTTTCAACAACACGTTCAGTACTGTGTTATCAACAAAAACATTGCAAATCCTTTTTTTAGTAAATGCCAAATAACATATACTATTTATTAGAACCAGCTTGCTCAGGGCCTAATAAGAGGAGCTGCACCATACATCATGACATAGTTACCAGCTTCCTCTTCTTCAAATTCGTTATCATCATATTCTAATTCTTCAGGTTGGCTATTGTCAAAAGGGGATTTGGGTGGAGTACCATTGGGCAGGAGTTTCGTATTGGGGTTTTTCCATTGTCATAAATGCTCAGCGAGTTGTTTTGTCTTTAGCCACAGAAAGGGCATTGTGCGTTGGGTCACTAGGCAAGGTTTGTAGTCATTGAGAGAAGCTCATTTTCTAGGATGATTAAAGTGATTCCTAAGGGATTCTTGCAGGGTTGCTAATGTAATGAACCGCTTCGATAAAACCACTCTTCAACATACTATTTACTGTCTGATTATGTTTGGTATATCGTTGAGGGTATTTCAGAATTTTTTTATTCAAATTTTCTGGTAGAGGAAGAAACCTGGCTCTGAGTTGATGTTTCATCCTCAACTAGAAGTGTATGGGTTTTCCTTCCTTCATGAGTTCTATTCAGTTGAAATTGCTCACTAGGCTGCTGGTCCCCTCCACCCACAATTTCTGAGCAACTATTTTAACTCTTTTTTTCTTTTAGTATGTTCATATTCCCGTGCAATAATTTTCTACTGCGTGTACATTCAAGAAAATCTTCTATGTGATGGTGGCCATCAAAATATGATAGATCATCCTTTTGCTTGTATTGATTGGCTGAACACTTGAGGAGAATTTTCTGAATCCCAAACTTGTTGCACTACCCATAGTATAAATTTTTAAATTCTTCTTGTGTTTCACTCTTAGGTGCTGGTTCATGGATGAGCCCCAAGGCTTGCCAGACAAAGCCAAACTAATCAGAAGTTCTATTTTGGCATTTGCCATGCGAAATGAACTAATCAGGTTTTGTTAATCAGAAATGAAGAATAATTATAAAACAAATTTGAATTAGGAGCCTGAAGAGAAAGCATTGAAGTTTGCCAAATCCTAACATTGCCACACGATCTATTTAGCCCCACCAACGTACATGTGATTTTAGTATTTGGCATTCTGAAATAGGGATGAAGAGAGAAATGGCTCTATCTTGTCACTACTTGGACGCATGCCTTATGCAGCGCTTAGGTTTTCTGATAAAATCCTCGAAGACCATTTAAACAAGTGAATTTTTATTCTTCTAAATTCATTCCCCCCGAGCAGGTTGATCATTGAGTGGTTGGGGTTGGGGTTGGGGTGACGGAGAAATTTCACAAACATCTTACTTTTTATCCTACTTAAATAATATTTCCTTCGTCTATTGCCTTTTCTCTTACCCCTTTCTGGTATATTCTCTACAAATACTTCTTATCGACTCTTGATTAAGTCATAATCAGTCTGCAGAAATAGACTCCAAGTGTCATTTTCTTTCCTATTTATTTCTTCTCTCTCTTATTTATTTATTTGTTTATTTTTTAGAATGGGTGTTTTGTTCTGATGCTATTATTCATTGAGAAATCTTTTCAGTCAAGCTAGTTAGTTGTAGTTTGTAATGCATGTTTTTACTTTTAGGTCCTTGTGGAAAAGGGAAAAAATGTTCAACATAATGGCAAACTAGAGGAGAAATAACTACATTTTCTCCATTTAGTTTTGAGTGTCACATCTTAGTTATATAACAAATGGCGTCTTATATTTCAGGGTTTTCGGAAAATTGAGACAGATTGCTGGGAATTTGCAACTGATGGATTCGTTAAAGGACAAAAACATCTGTTGAAGAACATCTACCGAAGGAAAAACATACAGGGCACAGATCAGCGTAGAGCGTCACAGCCCCAAGACAATTCTGAGGGACAAGGTGAACTACATGATTATACAGGGCTGTGGAAGGAAGTTGAGAATCTTAAAATAGACAAAAATGGTGTAATGCAGGAATTGGTTAAACTTAGGCAGCAGCAGGAAACTTCAGAGAGCAAGATGCTCCTATTGAGAGACCGCCTTCAAGGTATGGAAAAGAATCAACAGCAGATGCTATCATTCTTAGTAATGGCAGTGCAAAGCCCTGGATTTTTGGTTCAGTTTCTTCAACCAAAAGAGAAGAATTGGCGCATGGCTGATGCTGGCAATATGTTAGAACAAATTCCAGATGATAATCAAGTACCTTCAAATGGTTTGATAGTCAGGTATCAACGTCCAATAGATGAACTGCCAGCACCTCTACTTCCACCAGTGTCGGGTCTGGGGAAACAACAAGAATCTGATCCATTCCCTGATGGAATGAAGGACTTTTTCCTTAATTCCGACTTCATGAAAGTACTTATGGATGAAAAGTTAGACAATCACAGCCAATTTGTTCTACCAGATGTACAGGATGTCGCATGGGAGCAGCTTCTTTTAGCGAGCACTGTTTCTTGTAATTCAGATAATGTTAGAAAAGTTGACGACGAACGGGAGCCTATGGACCATGAGGACCATGAACTGGATATGGAAACAATTGATACTCGAACTCATGAAGAGAATCTGCAAGATTTCGAACTTCTAATCAAACAAATGGAGAAAGGTGAAAATTTCGGGTTGCAACCAAGGTTGGATGAATCGTATATCGAGAAATCAAATACTGTTAATCTTATGGAACTTATGGCATCTGATCAAGAAATCCTATATGAAACACCGGCGAAGATGCAAGGCCATTAAAAGAATTATATGTTTTTCTGGCGGGTTTATGTTTCTTCATGGATTTAAAATCTCAAAGATCAGTACAAGTTTCTTTTGTCTTCACCATCCATACCAAGTAACCATTGGTTGGCTATGAAGTTATGGTGATAAGCTGCTGCTGCTATTTCAATGTCTTGAGCAGAGTATTGAATTTCAAACAGGTTTAGGAATTTCGGCCATCTTTTTCTTGGTGATAACCTCAAGGTAATGTCTTTGTTTCAACTTGCAAGCATTATATCTTGTGGGCGTTGTTACAATTTTAATGCACACACAGTCTATATATATATATATATATATATTATCAATTTTGAAGAAGTGGTTTCTCAACTGCTTCTTGCCTGCCTTTGCCATATGTTTTTCTGTATAGCTTGGCAGACATTCCTCTTGTTATGATCACTAGATATTAAATTTATTTGATGTTCATTCTCTCTCTTGCATGTGCGTGCTCATGTACGCATATCTCACGCGAGATTTGTTCATTTTTCTTTATTCTTTGAATATTTGTAATGTTTTGATTGCTTTTTAAATTAGTCATTTTTAAGTTATTAGTTAAAACTTTTGCTTGAAAAATGATAAATATAGATTTATGTATTCATTCCTATATTTTGGTATAAGGCTTTCAACACTAAAGATCATATCTATATTTTGTAGACTATAGCAACTAGATACTCAGAAAATGAAACATAAAATCCAAAATGTTACTGAAAAAATGTACGATCACACATCACCTGAGTCATGAACCTCATGCAACTTCAAAATTGGCACATCGCAAAATACCATAATGGGTGGTCACCTAAGCATACGAAAAATTAAAATTCAAATTAGAAAAACTGACTTTTATTATCCTCAATCAAGTGACTAGCACAGCCAAGTCACCTTTCCAAGGGAATACAATATCCAAACCGAAAATAATGGTTGAATTACGAACTTAGACCATAAACTTATAGAAAAAATTTAATTTTGTGTATAATAAGTCTATAAACTAAAAAAAATGTTGTAATAAGTCATATAAATTTTTATTTCCTAAACTTTAAATTACATTTTTAAATTTTTTTTGTTTAGATCTTTCAAAGCTTAAGTTCAAAACTGTAACTGAGAAACACTTGCCGATCCTTCATAACTTTTTTAGTATTAAAAGAAATATACAATTTTCTTTAGAAAGTTCACCAAAAAGGAAGAAACTCGAGTTTTAAATGAATTGGACAAAATGCATGCTTTTAACTTAGCTGTTCTTTCTCTCCATGATTGAAGGCCGATGTCCATTTCACATTTTCCCTCTTCTCTACAGAAAATGAGAGAGGATTCATCTTATTGTTAATAGGAATTTACAGAAAGGGAGATTACAATTACGAAGCAGAAAAAACTAGGAACTCCGCACATCCCTCTAACCTCTGCTCTTCGTGTACAGGTTCGTATACACGACACTGGAAGCACGTGCCTCTAATCCAAGGAGAATGCTCCATTGCAGTACCTGTAGAGCCCAAATACAAAATAACTTCTCAGTTTGTAGGCAAAGACAGAGATTTTTCATTTTTTTGGCAAAAATCCTCTTTGTGCAGCTTCTTGACATAGGCCAAGTAAACTTTTATGTAACTACTCTCTTTGCAATGTTCTGACCAAGCTCGTTTGCCTTAGGGGTGGCTCCAGTATTTTTCATCCATCTTATGACATATTCCATTTCTCTTAGTAAAATAAGGGCCCTTTCATCATCCGTC

mRNA sequence

GAGAAGCGTCCACTGACATGCCTGCCTCTCAATAACCCAACCACACGACGCGGCGAAAAAGGGATAAACCCCAAAATTCTTCCTGTTCTACCCAATTACCCGAATCATTTTTCTATGCTTTCCTTCTAATTTCTTCCTTTATCCTTTTGCTTTCCTCCTAATTACGCTATCTAATCTCATCGGACTTCGAGAATTTTGCGATGGTGAAATCCGCGGGGGAGCGGCAGGGATCTTCGGTGGCGCCTTTTCTGAAGAAATTGTACGAGATGGTCGACGATGAGGCAACCAATTCCATTATCTCGTGGAGTCCGAGGGATGATAGCTTTACGATTCTGGACATGACTCATTTCTCTGTCCACTTGCTTCCCAAGTACTTTAAGCACAGTAATTTCTCTAGCTTTATGCGGCAGCTCAACATCTATGGTTTTCGGAAAATTGAGACAGATTGCTGGGAATTTGCAACTGATGGATTCGTTAAAGGACAAAAACATCTGTTGAAGAACATCTACCGAAGGAAAAACATACAGGGCACAGATCAGCGTAGAGCGTCACAGCCCCAAGACAATTCTGAGGGACAAGGTGAACTACATGATTATACAGGGCTGTGGAAGGAAGTTGAGAATCTTAAAATAGACAAAAATGGTGTAATGCAGGAATTGGTTAAACTTAGGCAGCAGCAGGAAACTTCAGAGAGCAAGATGCTCCTATTGAGAGACCGCCTTCAAGGTATGGAAAAGAATCAACAGCAGATGCTATCATTCTTAGTAATGGCAGTGCAAAGCCCTGGATTTTTGGTTCAGTTTCTTCAACCAAAAGAGAAGAATTGGCGCATGGCTGATGCTGGCAATATGTTAGAACAAATTCCAGATGATAATCAAGTACCTTCAAATGGTTTGATAGTCAGGTATCAACGTCCAATAGATGAACTGCCAGCACCTCTACTTCCACCAGTGTCGGGTCTGGGGAAACAACAAGAATCTGATCCATTCCCTGATGGAATGAAGGACTTTTTCCTTAATTCCGACTTCATGAAAGTACTTATGGATGAAAAGTTAGACAATCACAGCCAATTTGTTCTACCAGATGTACAGGATGTCGCATGGGAGCAGCTTCTTTTAGCGAGCACTGTTTCTTGTAATTCAGATAATGTTAGAAAAGTTGACGACGAACGGGAGCCTATGGACCATGAGGACCATGAACTGGATATGGAAACAATTGATACTCGAACTCATGAAGAGAATCTGCAAGATTTCGAACTTCTAATCAAACAAATGGAGAAAGGTGAAAATTTCGGGTTGCAACCAAGGTTGGATGAATCGTATATCGAGAAATCAAATACTGTTAATCTTATGGAACTTATGGCATCTGATCAAGAAATCCTATATGAAACACCGGCGAAGATGCAAGGCCATTAAAAGAATTATATGTTTTTCTGGCGGGTTTATGTTTCTTCATGGATTTAAAATCTCAAAGATCAGTACAAGTTTCTTTTGTCTTCACCATCCATACCAAGTAACCATTGGTTGGCTATGAAGTTATGGTGATAAGCTGCTGCTGCTATTTCAATGTCTTGAGCAGAGTATTGAATTTCAAACAGGTTTAGGAATTTCGGCCATCTTTTTCTTGGTGATAACCTCAAGGTTCGTATACACGACACTGGAAGCACGTGCCTCTAATCCAAGGAGAATGCTCCATTGCAGTACCTGTAGAGCCCAAATACAAAATAACTTCTCAGTTTGTAGGCAAAGACAGAGATTTTTCATTTTTTTGGCAAAAATCCTCTTTGTGCAGCTTCTTGACATAGGCCAAGTAAACTTTTATGTAACTACTCTCTTTGCAATGTTCTGACCAAGCTCGTTTGCCTTAGGGGTGGCTCCAGTATTTTTCATCCATCTTATGACATATTCCATTTCTCTTAGTAAAATAAGGGCCCTTTCATCATCCGTC

Coding sequence (CDS)

ATGGTGAAATCCGCGGGGGAGCGGCAGGGATCTTCGGTGGCGCCTTTTCTGAAGAAATTGTACGAGATGGTCGACGATGAGGCAACCAATTCCATTATCTCGTGGAGTCCGAGGGATGATAGCTTTACGATTCTGGACATGACTCATTTCTCTGTCCACTTGCTTCCCAAGTACTTTAAGCACAGTAATTTCTCTAGCTTTATGCGGCAGCTCAACATCTATGGTTTTCGGAAAATTGAGACAGATTGCTGGGAATTTGCAACTGATGGATTCGTTAAAGGACAAAAACATCTGTTGAAGAACATCTACCGAAGGAAAAACATACAGGGCACAGATCAGCGTAGAGCGTCACAGCCCCAAGACAATTCTGAGGGACAAGGTGAACTACATGATTATACAGGGCTGTGGAAGGAAGTTGAGAATCTTAAAATAGACAAAAATGGTGTAATGCAGGAATTGGTTAAACTTAGGCAGCAGCAGGAAACTTCAGAGAGCAAGATGCTCCTATTGAGAGACCGCCTTCAAGGTATGGAAAAGAATCAACAGCAGATGCTATCATTCTTAGTAATGGCAGTGCAAAGCCCTGGATTTTTGGTTCAGTTTCTTCAACCAAAAGAGAAGAATTGGCGCATGGCTGATGCTGGCAATATGTTAGAACAAATTCCAGATGATAATCAAGTACCTTCAAATGGTTTGATAGTCAGGTATCAACGTCCAATAGATGAACTGCCAGCACCTCTACTTCCACCAGTGTCGGGTCTGGGGAAACAACAAGAATCTGATCCATTCCCTGATGGAATGAAGGACTTTTTCCTTAATTCCGACTTCATGAAAGTACTTATGGATGAAAAGTTAGACAATCACAGCCAATTTGTTCTACCAGATGTACAGGATGTCGCATGGGAGCAGCTTCTTTTAGCGAGCACTGTTTCTTGTAATTCAGATAATGTTAGAAAAGTTGACGACGAACGGGAGCCTATGGACCATGAGGACCATGAACTGGATATGGAAACAATTGATACTCGAACTCATGAAGAGAATCTGCAAGATTTCGAACTTCTAATCAAACAAATGGAGAAAGGTGAAAATTTCGGGTTGCAACCAAGGTTGGATGAATCGTATATCGAGAAATCAAATACTGTTAATCTTATGGAACTTATGGCATCTGATCAAGAAATCCTATATGAAACACCGGCGAAGATGCAAGGCCATTAA

Protein sequence

MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPIDELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVAWEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEKGENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH
Homology
BLAST of CmaCh04G000870 vs. ExPASy Swiss-Prot
Match: Q9S7U5 (Heat stress transcription factor A-8 OS=Arabidopsis thaliana OX=3702 GN=HSFA8 PE=2 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 6.9e-77
Identity = 184/400 (46.00%), Postives = 243/400 (60.75%), Query Frame = 0

Query: 1   MVKS----AGERQGSSVAPFLKKLYEMVDDEATNSIISWSP-RDDSFTILDMTHFSVHLL 60
           MVKS     G    SSVAPFL+K Y+MVDD  T+SIISWSP  D+SF ILD T FSV LL
Sbjct: 1   MVKSTDGGGGSSSSSSVAPFLRKCYDMVDDSTTDSIISWSPSADNSFVILDTTVFSVQLL 60

Query: 61  PKYFKHSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRR 120
           PKYFKHSNFSSF+RQLNIYGFRK++ D WEFA DGFV+GQK LLKN+ RRKN+Q ++Q +
Sbjct: 61  PKYFKHSNFSSFIRQLNIYGFRKVDADRWEFANDGFVRGQKDLLKNVIRRKNVQSSEQSK 120

Query: 121 ASQPQDNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQ 180
                  S       + +GLWKEV+ LK DK  + QEL+K+RQ QE +++KML L DR+Q
Sbjct: 121 -----HESTSTTYAQEKSGLWKEVDILKGDKQVLAQELIKVRQYQEVTDTKMLHLEDRVQ 180

Query: 181 GMEKNQQQMLSFLVMAVQSPGFLVQFLQPKEKN-WRMADAG-NMLEQIPDDNQVPSNGL- 240
           GME++QQ+MLSFLVM +++P  LVQ LQPKEKN WR A  G  ++E++ D+ +  S GL 
Sbjct: 181 GMEESQQEMLSFLVMVMKNPSLLVQLLQPKEKNTWRKAGEGAKIVEEVTDEGESNSYGLP 240

Query: 241 IVRYQRPIDELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFV 300
           +V YQ P D          +G  K   +D     + DF  N+D +K  +DE   NH   +
Sbjct: 241 LVTYQPPSDN---------NGTAKSNSND-----VNDFLRNADMLKFCLDE---NHVPLI 300

Query: 301 LPDV-QDVAWEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDF 360
           +PD+  D AWE+LLL S     S    K  +       +D  L+ E  D     +     
Sbjct: 301 IPDLYDDGAWEKLLLLSP----SRKKTKKQENIVKKGKDDLTLEEEEEDGTMELDKSYML 360

Query: 361 ELLIKQMEKGENFGLQPRLDESYIEKSNTVNLMELMASDQ 392
           +L+ ++MEK ++F       E           MEL+AS++
Sbjct: 361 KLISEEMEKPDDFEFGQLTPERSRNLEILTEQMELLASNE 374

BLAST of CmaCh04G000870 vs. ExPASy Swiss-Prot
Match: Q40152 (Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 5.1e-56
Identity = 126/276 (45.65%), Postives = 175/276 (63.41%), Query Frame = 0

Query: 15  PFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K Y+MVDD +T+ I+SWSP ++SF + D   F+  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 39  PFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFVRQLNTY 98

Query: 75  GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELHDYT- 134
           GFRK++ D WEFA +GF++GQKHLLK+I RRK   G  Q++  QP  N++ Q +   ++ 
Sbjct: 99  GFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQ-QQPHGNAQQQMQPPGHSA 158

Query: 135 -----------GLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQ 194
                      GL +EVE LK DKN +MQELV+LRQQQ+ +++++  +  RLQGME  QQ
Sbjct: 159 SVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQATDNQLQGMVQRLQGMELRQQ 218

Query: 195 QMLSFLVMAVQSPGFLVQFLQPK-EKNWRMADAGNMLE-----QIPDDNQVPSNGLIVRY 254
           QM+SFL  AV  PGFL QF+Q + E N R+A+           +  D +  P++G IV+Y
Sbjct: 219 QMMSFLAKAVNRPGFLAQFVQQQNESNKRIAEGSKKRRIKQDIESQDPSVTPADGQIVKY 278

Query: 255 QRPIDELPAPLLPPVSGLGKQQESDPFPDGMKDFFL 273
           Q  I+E    +L  +S L      D F +  + F +
Sbjct: 279 QPGINEAAKAMLRELSKLDSSPRLDNFSNSPESFLI 313

BLAST of CmaCh04G000870 vs. ExPASy Swiss-Prot
Match: Q84T61 (Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA1 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 8.7e-56
Identity = 126/270 (46.67%), Postives = 170/270 (62.96%), Query Frame = 0

Query: 15  PFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K YEMVDD AT++++SW P ++SF + +   F+  LLPKYFKHSNFSSF+RQLN Y
Sbjct: 37  PFLMKTYEMVDDPATDAVVSWGPGNNSFVVWNTPEFARDLLPKYFKHSNFSSFVRQLNTY 96

Query: 75  GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQ-PQDNSEGQGELHDYT 134
           GFRK++ D WEFA +GF++GQKHLLK I RRK   G +Q +  Q P        E+  + 
Sbjct: 97  GFRKVDPDRWEFANEGFLRGQKHLLKTINRRKPTHGNNQVQQPQLPAAPVPACVEVGKF- 156

Query: 135 GLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVMAVQ 194
           G+ +E+E LK DKN +MQELV+LRQQQ+T++ ++  L  RLQGME+ QQQM+SFL  A+ 
Sbjct: 157 GMEEEIEMLKRDKNVLMQELVRLRQQQQTTDHQLQTLGKRLQGMEQRQQQMMSFLAKAMH 216

Query: 195 SPGFLVQFLQPKEKNWRMADAGNMLEQIP------DDNQVPSNGLIVRYQRPIDELPAPL 254
           SPGFL QF+Q  E + R   A N   ++P      D      +G IV+YQ  I+E    +
Sbjct: 217 SPGFLAQFVQQNENSRRRIVASNKKRRLPKQDGSLDSESASLDGQIVKYQPMINEAAKAM 276

Query: 255 LPPVSGLGKQQESDPFPDGMKDFFLNSDFM 278
           L  +  L      +    G  D FL  ++M
Sbjct: 277 LRKILKLDSSHRFESM--GNSDNFLLENYM 303

BLAST of CmaCh04G000870 vs. ExPASy Swiss-Prot
Match: P41153 (Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-55
Identity = 125/276 (45.29%), Postives = 177/276 (64.13%), Query Frame = 0

Query: 15  PFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K Y+MVDD +T+ I+SWSP ++SF + D   F+  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 41  PFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFVRQLNTY 100

Query: 75  GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELHDYT- 134
           GFRK++ D WEFA +GF++GQKHLLK+I RRK   G  Q++  QP  +++ Q +   ++ 
Sbjct: 101 GFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQ-QQPHGHAQQQMQPPGHSA 160

Query: 135 -----------GLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQ 194
                      GL +EVE LK DKN +MQELV+LRQQQ+++++++  +  RLQGME  QQ
Sbjct: 161 SVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQGMVQRLQGMELRQQ 220

Query: 195 QMLSFLVMAVQSPGFLVQFLQPK-EKNWRMADAGNMLE-----QIPDDNQVPSNGLIVRY 254
           QM+SFL  AV SPGFL QF+Q + E N R+A+           +  D +  P++G IV+Y
Sbjct: 221 QMMSFLAKAVNSPGFLAQFVQQQNESNKRIAEGSKKRRIKQDIESQDPSVTPADGQIVKY 280

Query: 255 QRPIDELPAPLLPPVSGLGKQQESDPFPDGMKDFFL 273
           Q  I+E    +L  +S L      + F +  + F +
Sbjct: 281 QPGINEAAKAMLRELSKLDSSPRLENFSNSPESFLI 315

BLAST of CmaCh04G000870 vs. ExPASy Swiss-Prot
Match: P41151 (Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A PE=1 SV=2)

HSP 1 Score: 212.6 bits (540), Expect = 8.2e-54
Identity = 119/244 (48.77%), Postives = 162/244 (66.39%), Query Frame = 0

Query: 15  PFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K Y+MV+D AT++I+SWSP ++SF + D   FS  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 52  PFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLNTY 111

Query: 75  GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRAS-QPQDNSEGQGELHDYT 134
           GFRK++ D WEFA +GF++GQKHLLK I RRK++QG     ++ Q Q  S+GQG +   +
Sbjct: 112 GFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAALS 171

Query: 135 --------GLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQML 194
                   GL +EVE LK DKN +MQELVKLRQQQ+T+++K+ +L   LQ ME+ QQQ++
Sbjct: 172 SCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQIM 231

Query: 195 SFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQV------------PSNGLI 238
           SFL  AVQ+P FL QF+Q +  +       N   ++ +D+               S+G I
Sbjct: 232 SFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLREDSTAATESNSHSHSLEASDGQI 291

BLAST of CmaCh04G000870 vs. ExPASy TrEMBL
Match: A0A6J1K754 (heat stress transcription factor A-8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491273 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.3e-229
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300
           DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360
           WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360

Query: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH
Sbjct: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 404

BLAST of CmaCh04G000870 vs. ExPASy TrEMBL
Match: A0A6J1FNX4 (heat stress transcription factor A-8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447493 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 5.8e-228
Identity = 401/404 (99.26%), Postives = 403/404 (99.75%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQR+ASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKN VMQELVKLRQQQETSE+KMLLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300
           DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360
           WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360

Query: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH
Sbjct: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 404

BLAST of CmaCh04G000870 vs. ExPASy TrEMBL
Match: A0A6J1K088 (heat stress transcription factor A-8-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491273 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 6.0e-185
Identity = 330/332 (99.40%), Postives = 331/332 (99.70%), Query Frame = 0

Query: 73  IYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELHDY 132
           + GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELHDY
Sbjct: 11  LMGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELHDY 70

Query: 133 TGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVMAV 192
           TGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVMAV
Sbjct: 71  TGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVMAV 130

Query: 193 QSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPIDELPAPLLPPVS 252
           QSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPIDELPAPLLPPVS
Sbjct: 131 QSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPIDELPAPLLPPVS 190

Query: 253 GLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVAWEQLLLASTVSC 312
           GLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVAWEQLLLASTVSC
Sbjct: 191 GLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVAWEQLLLASTVSC 250

Query: 313 NSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEKGENFGLQPRLDE 372
           NSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEKGENFGLQPRLDE
Sbjct: 251 NSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEKGENFGLQPRLDE 310

Query: 373 SYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           SYIEKSNTVNLMELMASDQEILYETPAKMQGH
Sbjct: 311 SYIEKSNTVNLMELMASDQEILYETPAKMQGH 342

BLAST of CmaCh04G000870 vs. ExPASy TrEMBL
Match: A0A1S3CMJ8 (heat stress transcription factor A-8 OS=Cucumis melo OX=3656 GN=LOC103502515 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.7e-184
Identity = 336/408 (82.35%), Postives = 369/408 (90.44%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSA +RQGSSVAPFLKKLY+MVDD++TNSIISW+  +DSFTILD+T FS+HLLPKYFK
Sbjct: 1   MVKSAADRQGSSVAPFLKKLYDMVDDDSTNSIISWTSSNDSFTILDITQFSLHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGFVKGQKHLLK+IYRRKNIQGTDQR+A QP+
Sbjct: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKSIYRRKNIQGTDQRKALQPR 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNSEGQ EL DY+GLWKEVENLKIDKN VMQELVKL+Q QETSE+K+LLLR+RLQGMEKN
Sbjct: 121 DNSEGQVELPDYSGLWKEVENLKIDKNAVMQELVKLKQHQETSENKLLLLRERLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEK+WRMAD  NMLEQI DDNQVPSNG+IVRYQRP+
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKSWRMADPSNMLEQISDDNQVPSNGMIVRYQRPL 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEK--LDNHSQFVLPDVQD 300
           DEL   LLPPV+G GKQQES+PFPDGMKDFFLNSDFMKVLMDEK  LDNHSQFVLPDVQD
Sbjct: 241 DELSTTLLPPVTGPGKQQESEPFPDGMKDFFLNSDFMKVLMDEKVCLDNHSQFVLPDVQD 300

Query: 301 VAWEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQM 360
           VAWEQLLLA+  S NS+N RKVD ER   D ED ELDMETIDT+THEEN QDFELLI+QM
Sbjct: 301 VAWEQLLLANPFSGNSENGRKVDHERRYTDSEDDELDMETIDTQTHEENSQDFELLIRQM 360

Query: 361 EKGENFGLQPRLDESYIEKSNTVNL----MELMASDQEILYETPAKMQ 403
           EK E+F +Q RLDESYIE SN V+L    M+L+ASD+EILYETP KM+
Sbjct: 361 EKCEDFEIQTRLDESYIENSNDVHLLTQQMDLLASDREILYETPDKMR 408

BLAST of CmaCh04G000870 vs. ExPASy TrEMBL
Match: A0A6J1FQB1 (heat stress transcription factor A-8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447493 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.5e-183
Identity = 327/332 (98.49%), Postives = 330/332 (99.40%), Query Frame = 0

Query: 73  IYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELHDY 132
           + GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQR+ASQPQDNSEGQGELHDY
Sbjct: 11  LMGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQDNSEGQGELHDY 70

Query: 133 TGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVMAV 192
           TGLWKEVENLKIDKN VMQELVKLRQQQETSE+KMLLLRDRLQGMEKNQQQMLSFLVMAV
Sbjct: 71  TGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKNQQQMLSFLVMAV 130

Query: 193 QSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPIDELPAPLLPPVS 252
           QSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPIDELPAPLLPPVS
Sbjct: 131 QSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPIDELPAPLLPPVS 190

Query: 253 GLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVAWEQLLLASTVSC 312
           GLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVAWEQLLLASTVSC
Sbjct: 191 GLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVAWEQLLLASTVSC 250

Query: 313 NSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEKGENFGLQPRLDE 372
           NSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEKGENFGLQPRLDE
Sbjct: 251 NSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEKGENFGLQPRLDE 310

Query: 373 SYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           SYIEKSNTVNLMELMASDQEILYETPAKMQGH
Sbjct: 311 SYIEKSNTVNLMELMASDQEILYETPAKMQGH 342

BLAST of CmaCh04G000870 vs. NCBI nr
Match: XP_022995879.1 (heat stress transcription factor A-8-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 804.3 bits (2076), Expect = 4.8e-229
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300
           DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360
           WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360

Query: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH
Sbjct: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 404

BLAST of CmaCh04G000870 vs. NCBI nr
Match: XP_022942461.1 (heat stress transcription factor A-8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 799.7 bits (2064), Expect = 1.2e-227
Identity = 401/404 (99.26%), Postives = 403/404 (99.75%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQR+ASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKN VMQELVKLRQQQETSE+KMLLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300
           DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360
           WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360

Query: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH
Sbjct: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 404

BLAST of CmaCh04G000870 vs. NCBI nr
Match: XP_023536181.1 (heat stress transcription factor A-8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 794.7 bits (2051), Expect = 3.8e-226
Identity = 399/404 (98.76%), Postives = 401/404 (99.26%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQR+ASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKN VMQELVKLRQQQETSE+KMLLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300
           DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360
           WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQMEK 360

Query: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYET  KMQGH
Sbjct: 361 GENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETQPKMQGH 404

BLAST of CmaCh04G000870 vs. NCBI nr
Match: KAG6599929.1 (Heat stress transcription factor A-8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.2 bits (2042), Expect = 4.2e-225
Identity = 400/407 (98.28%), Postives = 402/407 (98.77%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQR+ASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKN VMQELVKLRQQQETSE+KMLLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300
           DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDH---EDHELDMETIDTRTHEENLQDFELLIKQ 360
           WEQLLLASTVSCNSDNVRKVDDEREPMDH   EDHELDMETIDTRTHEENLQDFELLIKQ
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDLEDHELDMETIDTRTHEENLQDFELLIKQ 360

Query: 361 MEKGENFGLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 405
           MEKGENF LQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH
Sbjct: 361 MEKGENFRLQPRLDESYIEKSNTVNLMELMASDQEILYETPAKMQGH 407

BLAST of CmaCh04G000870 vs. NCBI nr
Match: XP_038892281.1 (heat stress transcription factor A-8 [Benincasa hispida])

HSP 1 Score: 674.9 bits (1740), Expect = 4.4e-190
Identity = 347/408 (85.05%), Postives = 371/408 (90.93%), Query Frame = 0

Query: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60
           MVKSA ERQGSSVAPFLKKLY+MVDD++TNSIISW+   DSFTILD+T FS+H+LPKYFK
Sbjct: 1   MVKSAAERQGSSVAPFLKKLYDMVDDDSTNSIISWTSSHDSFTILDITQFSLHVLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGF+KGQKHLLKNIYRRKNIQGTDQRRASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFIKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180
           DNS+GQ EL DY+GLWKEVENLKIDKN VMQELVKL+Q QETSE+K+LLLRDRLQGMEKN
Sbjct: 121 DNSDGQVELPDYSGLWKEVENLKIDKNAVMQELVKLKQHQETSENKLLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQ LQPKEKNWRMAD GNMLEQIPDDNQVPSNG+IVRYQRP+
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQLLQPKEKNWRMADTGNMLEQIPDDNQVPSNGMIVRYQRPL 240

Query: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEK--LDNHSQFVLPDVQD 300
           DEL   LLP V+G GKQQESDPFPDGMKDFFLNSDFMKVLMDEK  LDNHSQFVLPDVQD
Sbjct: 241 DELSTTLLPAVTGPGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLCLDNHSQFVLPDVQD 300

Query: 301 VAWEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDFELLIKQM 360
           VAWEQLLLAS  S NSDN RK+DDER  MD ED ELDMETIDT+THEEN QDFELLI+QM
Sbjct: 301 VAWEQLLLASPFSGNSDNGRKIDDERRHMDDEDDELDMETIDTQTHEENSQDFELLIRQM 360

Query: 361 EKGENFGLQPRLDESYIEKSNTVNL----MELMASDQEILYETPAKMQ 403
           EK E+F +QPRLDESYIEK NTVNL    ME +ASDQEILYET  K+Q
Sbjct: 361 EKCEDFEIQPRLDESYIEKLNTVNLLTQQMEPLASDQEILYETADKIQ 408

BLAST of CmaCh04G000870 vs. TAIR 10
Match: AT1G67970.1 (heat shock transcription factor A8 )

HSP 1 Score: 289.3 bits (739), Expect = 4.9e-78
Identity = 184/400 (46.00%), Postives = 243/400 (60.75%), Query Frame = 0

Query: 1   MVKS----AGERQGSSVAPFLKKLYEMVDDEATNSIISWSP-RDDSFTILDMTHFSVHLL 60
           MVKS     G    SSVAPFL+K Y+MVDD  T+SIISWSP  D+SF ILD T FSV LL
Sbjct: 1   MVKSTDGGGGSSSSSSVAPFLRKCYDMVDDSTTDSIISWSPSADNSFVILDTTVFSVQLL 60

Query: 61  PKYFKHSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRR 120
           PKYFKHSNFSSF+RQLNIYGFRK++ D WEFA DGFV+GQK LLKN+ RRKN+Q ++Q +
Sbjct: 61  PKYFKHSNFSSFIRQLNIYGFRKVDADRWEFANDGFVRGQKDLLKNVIRRKNVQSSEQSK 120

Query: 121 ASQPQDNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQ 180
                  S       + +GLWKEV+ LK DK  + QEL+K+RQ QE +++KML L DR+Q
Sbjct: 121 -----HESTSTTYAQEKSGLWKEVDILKGDKQVLAQELIKVRQYQEVTDTKMLHLEDRVQ 180

Query: 181 GMEKNQQQMLSFLVMAVQSPGFLVQFLQPKEKN-WRMADAG-NMLEQIPDDNQVPSNGL- 240
           GME++QQ+MLSFLVM +++P  LVQ LQPKEKN WR A  G  ++E++ D+ +  S GL 
Sbjct: 181 GMEESQQEMLSFLVMVMKNPSLLVQLLQPKEKNTWRKAGEGAKIVEEVTDEGESNSYGLP 240

Query: 241 IVRYQRPIDELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFV 300
           +V YQ P D          +G  K   +D     + DF  N+D +K  +DE   NH   +
Sbjct: 241 LVTYQPPSDN---------NGTAKSNSND-----VNDFLRNADMLKFCLDE---NHVPLI 300

Query: 301 LPDV-QDVAWEQLLLASTVSCNSDNVRKVDDEREPMDHEDHELDMETIDTRTHEENLQDF 360
           +PD+  D AWE+LLL S     S    K  +       +D  L+ E  D     +     
Sbjct: 301 IPDLYDDGAWEKLLLLSP----SRKKTKKQENIVKKGKDDLTLEEEEEDGTMELDKSYML 360

Query: 361 ELLIKQMEKGENFGLQPRLDESYIEKSNTVNLMELMASDQ 392
           +L+ ++MEK ++F       E           MEL+AS++
Sbjct: 361 KLISEEMEKPDDFEFGQLTPERSRNLEILTEQMELLASNE 374

BLAST of CmaCh04G000870 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 212.6 bits (540), Expect = 5.8e-55
Identity = 119/244 (48.77%), Postives = 162/244 (66.39%), Query Frame = 0

Query: 15  PFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K Y+MV+D AT++I+SWSP ++SF + D   FS  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 52  PFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLNTY 111

Query: 75  GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRAS-QPQDNSEGQGELHDYT 134
           GFRK++ D WEFA +GF++GQKHLLK I RRK++QG     ++ Q Q  S+GQG +   +
Sbjct: 112 GFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAALS 171

Query: 135 --------GLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQML 194
                   GL +EVE LK DKN +MQELVKLRQQQ+T+++K+ +L   LQ ME+ QQQ++
Sbjct: 172 SCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQIM 231

Query: 195 SFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQV------------PSNGLI 238
           SFL  AVQ+P FL QF+Q +  +       N   ++ +D+               S+G I
Sbjct: 232 SFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLREDSTAATESNSHSHSLEASDGQI 291

BLAST of CmaCh04G000870 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 203.8 bits (517), Expect = 2.7e-52
Identity = 118/247 (47.77%), Postives = 159/247 (64.37%), Query Frame = 0

Query: 11  SSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQ 70
           +SV PFL K Y+MVDD  TN ++SWS  ++SF +     FS  LLPKYFKH+NFSSF+RQ
Sbjct: 23  NSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQ 82

Query: 71  LNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELH 130
           LN YGFRK++ D WEFA +GF++G+K LLK+I RRK       ++ +Q Q +S G     
Sbjct: 83  LNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEV 142

Query: 131 DYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVM 190
              G+ +EVE LK DKN +MQELV+LRQQQ+ +E+++  +  ++Q ME+ QQQM+SFL  
Sbjct: 143 GKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAK 202

Query: 191 AVQSPGFLVQFLQPKEKNW-RMADAGNMLEQIPDDNQV-----PSNGL---IVRYQRPID 249
           AVQSPGFL Q +Q    +  R     N   ++P D Q       +NGL   IVRYQ  I+
Sbjct: 203 AVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSIN 262

BLAST of CmaCh04G000870 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 203.8 bits (517), Expect = 2.7e-52
Identity = 118/247 (47.77%), Postives = 159/247 (64.37%), Query Frame = 0

Query: 11  SSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQ 70
           +SV PFL K Y+MVDD  TN ++SWS  ++SF +     FS  LLPKYFKH+NFSSF+RQ
Sbjct: 23  NSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQ 82

Query: 71  LNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQGELH 130
           LN YGFRK++ D WEFA +GF++G+K LLK+I RRK       ++ +Q Q +S G     
Sbjct: 83  LNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEV 142

Query: 131 DYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSFLVM 190
              G+ +EVE LK DKN +MQELV+LRQQQ+ +E+++  +  ++Q ME+ QQQM+SFL  
Sbjct: 143 GKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAK 202

Query: 191 AVQSPGFLVQFLQPKEKNW-RMADAGNMLEQIPDDNQV-----PSNGL---IVRYQRPID 249
           AVQSPGFL Q +Q    +  R     N   ++P D Q       +NGL   IVRYQ  I+
Sbjct: 203 AVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSIN 262

BLAST of CmaCh04G000870 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 200.7 bits (509), Expect = 2.3e-51
Identity = 121/276 (43.84%), Postives = 170/276 (61.59%), Query Frame = 0

Query: 15  PFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K Y+MVDD  T+SI+SWS  ++SF +     F+  LLPK FKH+NFSSF+RQLN Y
Sbjct: 37  PFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSFVRQLNTY 96

Query: 75  GFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQDNSEGQG------- 134
           GFRK++ D WEFA +GF++GQKHLL++I RRK   G  Q +  Q   +S GQ        
Sbjct: 97  GFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHG--QGQGHQRSQHSNGQNSSVSACV 156

Query: 135 ELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKNQQQMLSF 194
           E+  + GL +EVE LK DKN +MQELV+LRQQQ+++++++  +  RLQGME  QQQ++SF
Sbjct: 157 EVGKF-GLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQTMVQRLQGMENRQQQLMSF 216

Query: 195 LVMAVQSPGFLVQFLQPK----EKNWRMADAGNMLE-----QIPDDNQVPSNGLIVRYQR 254
           L  AVQSP FL QFLQ +    E N R++D            + +++    +G IV+YQ 
Sbjct: 217 LAKAVQSPHFLSQFLQQQNQQNESNRRISDTSKKRRFKRDGIVRNNDSATPDGQIVKYQP 276

Query: 255 PIDELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNS 275
           P+ E    +   +       + +P+  G   F L +
Sbjct: 277 PMHEQAKAMFKQL------MKMEPYKTGDDGFLLGN 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7U56.9e-7746.00Heat stress transcription factor A-8 OS=Arabidopsis thaliana OX=3702 GN=HSFA8 PE... [more]
Q401525.1e-5645.65Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1[more]
Q84T618.7e-5646.67Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P411531.1e-5545.29Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1[more]
P411518.2e-5448.77Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A ... [more]
Match NameE-valueIdentityDescription
A0A6J1K7542.3e-229100.00heat stress transcription factor A-8-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1FNX45.8e-22899.26heat stress transcription factor A-8-like isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A6J1K0886.0e-18599.40heat stress transcription factor A-8-like isoform X2 OS=Cucurbita maxima OX=3661... [more]
A0A1S3CMJ86.7e-18482.35heat stress transcription factor A-8 OS=Cucumis melo OX=3656 GN=LOC103502515 PE=... [more]
A0A6J1FQB11.5e-18398.49heat stress transcription factor A-8-like isoform X2 OS=Cucurbita moschata OX=36... [more]
Match NameE-valueIdentityDescription
XP_022995879.14.8e-229100.00heat stress transcription factor A-8-like isoform X1 [Cucurbita maxima][more]
XP_022942461.11.2e-22799.26heat stress transcription factor A-8-like isoform X1 [Cucurbita moschata][more]
XP_023536181.13.8e-22698.76heat stress transcription factor A-8-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
KAG6599929.14.2e-22598.28Heat stress transcription factor A-8, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_038892281.14.4e-19085.05heat stress transcription factor A-8 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G67970.14.9e-7846.00heat shock transcription factor A8 [more]
AT4G17750.15.8e-5548.77heat shock factor 1 [more]
AT5G16820.12.7e-5247.77heat shock factor 3 [more]
AT5G16820.22.7e-5247.77heat shock factor 3 [more]
AT1G32330.12.3e-5143.84heat shock transcription factor A1D [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 139..159
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..127
NoneNo IPR availablePANTHERPTHR10015:SF325HEAT STRESS TRANSCRIPTION FACTOR A-8coord: 1..344
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 67..79
score: 76.26
coord: 16..39
score: 46.19
coord: 54..66
score: 78.02
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 12..105
e-value: 8.7E-52
score: 188.0
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 16..105
e-value: 3.6E-30
score: 104.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 55..79
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 8..106
e-value: 2.8E-34
score: 119.7
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 1..344
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 11..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G000870.1CmaCh04G000870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding