CmaCh04G000750 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G000750
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	EF hand calcium-binding protein family
Location	Cma_Chr04: 357037 .. 357597 (+)
RNA-Seq Expression	CmaCh04G000750
Synteny	CmaCh04G000750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCACCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGCATGGACCCCGCTCCTCATCCGGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTATTCGACGCGGATGGCGATGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGCCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

mRNA sequence

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCACCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGCATGGACCCCGCTCCTCATCCGGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTATTCGACGCGGATGGCGATGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGCCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

Coding sequence (CDS)

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCACCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGCATGGACCCCGCTCCTCATCCGGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTATTCGACGCGGATGGCGATGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGCCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

Protein sequence

MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAGLSVAGG

Homology

[Close]

BLAST of CmaCh04G000750 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-50
Identity = 101/159 (63.52%), Postives = 127/159 (79.87%), Query Frame = 0


Query: 22  VSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELA 81
           + + S +  ++ SG +  R  ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL 
Sbjct: 18  IGADSPYLQKARSGKTEIR--ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELE 77

Query: 82  KMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVM 141
           K I   D  GDG+IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+V+
Sbjct: 78  KAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVL 137

Query: 142 RSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 181
           RSLGD+CS+A+CR+MI  VD++GDG I F+EFK+MM+ G
Sbjct: 138 RSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CmaCh04G000750 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 4.5e-39
Identity = 87/157 (55.41%), Postives = 112/157 (71.34%), Query Frame = 0


Query: 30  PRSSSGD-SHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFD 89
           P S  G  + +   E+E VF+KFD NGDG+IS +ELG++  SLGH AT++ELA+M+   D
Sbjct: 26  PASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEAD 85

Query: 90  ADGDGFINLQEFVELN-TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDD 149
           ADGDGFI+L EF  LN T   D   + E+LR AF V+D DGNG+ISA EL +V+  LG+ 
Sbjct: 86  ADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEK 145

Query: 150 CSVADCRQMISAVDRNGDGLISFDEFKVMMSAGLSVA 185
            +V  CR+MI  VD+NGDGLISF+EFKVMM+ G S A
Sbjct: 146 ATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGGSFA 182

[Close]

BLAST of CmaCh04G000750 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.9e-35
Identity = 73/139 (52.52%), Postives = 102/139 (73.38%), Query Frame = 0


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           E E VF+KFD NGDG+IS AEL ++  S+GH  T++E+A+M++  D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 103 LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVD 162
           ++     D     E+LR AF V+D DGNG I+  EL +V+R +G+  +VA CR+MI  VD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 163 RNGDGLISFDEFKVMMSAG 181
           RNGDGLI+F+EFK+MM+AG
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]

BLAST of CmaCh04G000750 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 6.2e-33
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 0


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 AVDRNGDGLISFDEFKVMMSAG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of CmaCh04G000750 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-32
Identity = 74/151 (49.01%), Postives = 98/151 (64.90%), Query Frame = 0


Query: 42  AELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFV 101
           AELE+VF+++D NGDGKIS+ EL S++ +LG      E+ +M++  D+D DGF++L EF 
Sbjct: 4   AELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFA 63

Query: 102 ELNTKEI-----------DPEELLE-NLREAFSVYDIDGNGSISAEELHKVMRSLGDDCS 161
             +               D E   E  LREAF +YD D NG ISA ELH+V+R LGD CS
Sbjct: 64  AFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCS 123

Query: 162 VADCRQMISAVDRNGDGLISFDEFKVMMSAG 181
           VADC +MI +VD +GDG ++FDEFK MM  G
Sbjct: 124 VADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]

BLAST of CmaCh04G000750 vs. ExPASy TrEMBL
Match: A0A6J1JH65 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111484331 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.7e-97
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG
Sbjct: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 186

[Close]

BLAST of CmaCh04G000750 vs. ExPASy TrEMBL
Match: A0A6J1FSG1 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111448020 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.0e-94
Identity = 181/186 (97.31%), Postives = 182/186 (97.85%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           MRFKCLFCWKKKPF SVTSSIVSSPST+GPRSSS DSHSRNAELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS VDRNGDGLISFDEFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSVG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 186

[Close]

BLAST of CmaCh04G000750 vs. ExPASy TrEMBL
Match: A0A5A7TBZ6 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold228G00230 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.4e-75
Identity = 157/186 (84.41%), Postives = 164/186 (88.17%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS   S S+ AELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMIE FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV+RSLGDDCSVADCRQMIS VD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 185

[Close]

BLAST of CmaCh04G000750 vs. ExPASy TrEMBL
Match: A0A1S3CNQ0 (probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.4e-75
Identity = 157/186 (84.41%), Postives = 164/186 (88.17%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS   S S+ AELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMIE FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV+RSLGDDCSVADCRQMIS VD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 185

[Close]

BLAST of CmaCh04G000750 vs. ExPASy TrEMBL
Match: A0A0A0KX11 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.0e-74
Identity = 154/185 (83.24%), Postives = 163/185 (88.11%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMI+ FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV++SLGDDCSVADCRQMIS VD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAG 186
           LSVAG
Sbjct: 181 LSVAG 184

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: XP_022986658.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 365.2 bits (936), Expect = 3.5e-97
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG
Sbjct: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 186

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: XP_023549905.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.8 bits (909), Expect = 4.7e-94
Identity = 181/186 (97.31%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           MRFKCLFCWKKKPF SVTSSIVSSPST+GPRSSS DSHSRNAELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELL+NLREA
Sbjct: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS VDRNGDGLISFDEFKVMMSAG
Sbjct: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 186

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: XP_022943227.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 354.4 bits (908), Expect = 6.1e-94
Identity = 181/186 (97.31%), Postives = 182/186 (97.85%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           MRFKCLFCWKKKPF SVTSSIVSSPST+GPRSSS DSHSRNAELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS VDRNGDGLISFDEFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSVG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 186

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: KAG7030595.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 354.4 bits (908), Expect = 6.1e-94
Identity = 181/186 (97.31%), Postives = 182/186 (97.85%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           MRFKCLFCWKKKPF SVTSSIVSSPST+GPRSSS DSHSRN ELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNTELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS VDRNGDGLISFDEFKVMMSAG
Sbjct: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 186

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: KAG6599916.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 352.4 bits (903), Expect = 2.3e-93
Identity = 180/186 (96.77%), Postives = 181/186 (97.31%), Query Frame = 0


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           MRFKCLFCWKKKPF SVTSSIVSSPST+GP SSS DSHSRN ELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPHSSSRDSHSRNTELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS VDRNGDGLISFDEFKVMMSAG
Sbjct: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 186

[Close]

BLAST of CmaCh04G000750 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 201.1 bits (510), Expect = 8.0e-52
Identity = 101/159 (63.52%), Postives = 127/159 (79.87%), Query Frame = 0


Query: 22  VSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELA 81
           + + S +  ++ SG +  R  ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL 
Sbjct: 18  IGADSPYLQKARSGKTEIR--ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELE 77

Query: 82  KMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVM 141
           K I   D  GDG+IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+V+
Sbjct: 78  KAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVL 137

Query: 142 RSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 181
           RSLGD+CS+A+CR+MI  VD++GDG I F+EFK+MM+ G
Sbjct: 138 RSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CmaCh04G000750 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 142.1 bits (357), Expect = 4.4e-34
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 0


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 AVDRNGDGLISFDEFKVMMSAG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of CmaCh04G000750 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-32
Identity = 66/137 (48.18%), Postives = 98/137 (71.53%), Query Frame = 0


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD N DGKIS  EL  ++G+L   A++EE   M++ FD DG+GFI+L EFV 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 103 L--NTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAV 162
           L   + +      + +L+EAF +YD+D NG ISA ELH VM++LG+ CS+ DC++MI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 163 DRNGDGLISFDEFKVMM 178
           D +GDG + F+EFK MM
Sbjct: 135 DSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CmaCh04G000750 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-30
Identity = 72/160 (45.00%), Postives = 103/160 (64.38%), Query Frame = 0


Query: 26  STHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIE 85
           +T+   ++   + S + EL++VF KFD NGDGKIS +ELG++  S+G   TEEEL ++++
Sbjct: 3   NTNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLD 62

Query: 86  VFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLG 145
             D D DGFIN +EF  +        E    +REAF +YD + NG IS+ E+HKV+  LG
Sbjct: 63  EIDIDCDGFINQEEFATICRSSSSAVE----IREAFDLYDQNKNGLISSSEIHKVLNRLG 122

Query: 146 DDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAGLSVAG 186
             CSV DC +MI  VD +GDG ++F+EF+ MMS+   V G
Sbjct: 123 MTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMSSPELVKG 158

[Close]

BLAST of CmaCh04G000750 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-29
Identity = 71/157 (45.22%), Postives = 99/157 (63.06%), Query Frame = 0


Query: 23  SSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAK 82
           ++P T  P  ++ D  +   EL++VF +FD NGDGKIS  ELG +  ++G   TE EL +
Sbjct: 4   ANPETAKPTPATVDM-ANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNR 63

Query: 83  MIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMR 142
           ++E  D D DG+INL EF  L        E    +R+AF +YD D NG ISA ELH+V+ 
Sbjct: 64  VLEEVDTDRDGYINLDEFSTLCRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLN 123

Query: 143 SLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSA 180
            LG  CSV DC +MI  VD +GDG ++F+EF+ MM++
Sbjct: 124 RLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTS 155

The following BLAST results are available for this feature:

BLAST of CmaCh04G000750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	1.1e-50	63.52	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	4.5e-39	55.41	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6L4D4	3.9e-35	52.52	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
P25070	6.2e-33	51.41	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q0DJV6	1.1e-32	49.01	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CmaCh04G000750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JH65	1.7e-97	100.00	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114843...	[more]
A0A6J1FSG1	3.0e-94	97.31	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7TBZ6	1.4e-75	84.41	Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CNQ0	1.4e-75	84.41	probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 P...	[more]
A0A0A0KX11	2.0e-74	83.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1	[more]

BLAST of CmaCh04G000750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986658.1	3.5e-97	100.00	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]
XP_023549905.1	4.7e-94	97.31	probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo]	[more]
XP_022943227.1	6.1e-94	97.31	probable calcium-binding protein CML25 [Cucurbita moschata]	[more]
KAG7030595.1	6.1e-94	97.31	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. a...	[more]
KAG6599916.1	2.3e-93	96.77	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of CmaCh04G000750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	8.0e-52	63.52	EF hand calcium-binding protein family	[more]
AT5G37770.1	4.4e-34	51.41	EF hand calcium-binding protein family	[more]
AT1G66400.1	1.1e-32	48.18	calmodulin like 23	[more]
AT1G73630.1	1.0e-30	45.00	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.1e-29	45.22	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 152..180 e-value: 3.7E-6 score: 36.4 coord: 43..71 e-value: 4.7E-7 score: 39.4 coord: 79..107 e-value: 7.2E-5 score: 32.2 coord: 116..144 e-value: 1.9E-6 score: 37.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 43..103 e-value: 1.6E-12 score: 47.6 coord: 115..177 e-value: 1.0E-13 score: 51.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 112..147 score: 15.740803
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 39..74 score: 15.796595
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 148..183 score: 13.369694
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 75..110 score: 13.341799
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 117..178 e-value: 4.01257E-20 score: 77.5881
None	No IPR available	GENE3D	1.10.238.10		coord: 11..106 e-value: 1.9E-23 score: 84.5 coord: 107..182 e-value: 8.9E-26 score: 92.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 19..40
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 19..37
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 23..180
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 23..180
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 88..100
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 125..137
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 52..64
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 161..173
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 39..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G000750.1	CmaCh04G000750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048767	root hair elongation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005509	calcium ion binding