CmaCh04G000520 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G000520
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description(3S,6E)-nerolidol synthase 1-like
LocationCma_Chr04: 257829 .. 260407 (+)
RNA-Seq ExpressionCmaCh04G000520
SyntenyCmaCh04G000520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACCTTCTCTTCTTTCTTCCTCCTCCTCCTCCTCCAATAACCTTAACCTTAATGTCTCACTCAAAGATTCCAAAAGCTTTTCACAACCATTCATGGCTTTTCAAGAACCCTATCGAGAAACCAGGTTTTGCAGATAAAAAATGGAGCATTCATGCGGATTCTTCTTCCTTCAATCTCTTAAACTTTGCTGTATGTTTGTAACTCTCTCCTTTCTCTCTATTTTCTCTCTATCTAAATTGCACTCCTTGTCTAAGTCCTTGGAATTGATACTCATGTGCATAGGATGGCCTTGGGCTTGTTGAAGCTCCAGCTAAAGTGGATATTTTAAGGCATTTATTGAGAGACCAAACTGGAGATTCCTTGGATTGCTTGAACCTTATTGATGCCACCCAACGCCTTGGCATTGATTACCACTTCCAACAGGAGATTCAAGTTGTTCTTCAAAGGCTATACGTCTTATCTAACCATCAATGGCTAGATCGCGAGACCAATCTTCATTATACTTCTCTTCTCTTTCGACTTTTGAGACAACAAGGTTATCCCGTGTCCACAGGTTACTACCAACTACTCTGTTATATCAACTAAAAGGACAAGCGAGCTACTTAGCCGCCCTAAACACAATCTCACTCTTTCTAATCTTTGTTCATAATTTGAACCAACAGATAATTTCAGAGCTTTCTTGGACAACAAGGGAAAGTTCAACGAAGGCCTAAGACAAAACGTGAATGGGCTGATGAGCTTATATGAAGCTTCACACCTATGCATGCATGGCGATGACATACTTGAAGAAGCTGAAACTTTCAGCACCCATGGCCTTAATCATTGCCTTGCTCATCTTGATCATCATATGGCCCCTTTTGTGCGCAACACTTTAGCTCATCCCCACCATAAAAGCGTTGCTAAATTCATGGTGCCTACCTATTTTGGAGACATTCAAAGCACAAACAAATGGATCCATGTTTTGCAAGATGTTGCCAAAATGGATTTCAATGCAGCTCAGCTGCTACACCAAGACGAACTTGTTCAGTTCTTGAAGTAACATTTTGTTTATCTATAATATTGTTGTCTTCATTAGACAAATTAAAAAAAAAAATCTTTAGATGCGTCCTCATGTTTCTTGATGTTTTGTTAATCATAGATGGTGGAAAGAAACAGGCTTGGCGAAAGAGTTGAAGTTTGCAAGAGATGAACCTATTAAATGGTACATAGAATCACTAGTTTGTCTCGGAGATTCATGCTATTCCCAACAAAGACTCCAACTTGCAAAGTCCATCTCTTTTGTGTATCTCATTGATGACCTTTATGATGTATTTGGAAATCTTAAAGAACTCACCTTCTTCACCGAAGCTGTCTGCAGGTTGGAAGCCATATTCGTGGCTGTTGTCATATCAAATTTCATGTTATTGAAATCAACATTGTTCACTACATTGTAGATGGGATTTGGGTGCTGCTAAAAGATTGCCTGATTGCATGGGAATTTGTTTAAAATCTCTATTTGAAGTTACCAATGATGTAAGTTCCATGATCTATAAGAAGCATGGCTGGAATCCCATTGGTTTCTTAAGTAGAGCGGTATGTATCTTTAATTTGTTGGATCAAGTTTGATCTCTATAACGTCACCCAAAATTGTGTTTTGACGTTTGTGTGCAGTGGGGTAAATTATGCAAGGCCTTCTTAGTGGAAACTGAATGGATGAGTTGTGGATATTCACCAAGTGCAGAAGAATATTTGAGGAATGGCATTGTTAGCACTGGCATCCACGTTATTTTAGTACATGTTTTCTTTCTATTAGGCCAACAAATAAGCCAGCAAACTGCAGAGCTTTTGGAAGATGATTTAGACATTATTTCATCTACTGCCATCATACTCCGACTCTGTGACGACATGGGCAGTGCAAAGGTAAAAATAAACTTACCCAACTTCAACCATGCATTCACTGTTTAAAACGGTAACGACCCATGTCAAGGATTCGAATTACGATTCAAAATTTGGATGATCCTGACATTTCCTTAAATCCCTTACGAAACATGATCATGTTGCTTACTCCTCCCTTTTAAAAGTGGAGACTATTCTCACCGACCAACACGAGTCATTCTAATATGATTTGGAAAAAGTTAGAATTTATGACTATTGATTTTAATGTTCGAACATAAACCAGTATGCATTTACACGTGTGCAGGACGAGAAGCAAGTGGGGCTAGATGGATCATATGCAAAACACTACATGAATGAGCATCCAAGCATCTCCCATGAAGCCACACACCATCATGTTACGAACAAAATTTCTCAAGCATGGAAGACTCTCAACAAAGAATATCTCTTATCCAACTTATTCCCAGCAAAGTTCACTCAGGCTTCTCTGAACATTGCAAGAGCAGTCCCTTTGGTTTATAATTATGGCTCAAACCAATCATTTATGACACTTGAAAAGCTCATACAACAATTGTTGTTTCGTATATATGTGTAACATTGGATCTTCATACACATTTATTACTACAAACTAAAACAAAACCATGATGAATACTCAAACAAAAATACACATTTATAATTGTTGTGTAATGATACTATCCTAG

mRNA sequence

ATGGCCAACCTTCTCTTCTTTCTTCCTCCTCCTCCTCCTCCAATAACCTTAACCTTAATGTCTCACTCAAAGATTCCAAAAGCTTTTCACAACCATTCATGGCTTTTCAAGAACCCTATCGAGAAACCAGGTTTTGCAGATAAAAAATGGAGCATTCATGCGGATTCTTCTTCCTTCAATCTCTTAAACTTTGCTGATGGCCTTGGGCTTGTTGAAGCTCCAGCTAAAGTGGATATTTTAAGGCATTTATTGAGAGACCAAACTGGAGATTCCTTGGATTGCTTGAACCTTATTGATGCCACCCAACGCCTTGGCATTGATTACCACTTCCAACAGGAGATTCAAGTTGTTCTTCAAAGGCTATACGTCTTATCTAACCATCAATGGCTAGATCGCGAGACCAATCTTCATTATACTTCTCTTCTCTTTCGACTTTTGAGACAACAAGGTTATCCCGTGTCCACAGATAATTTCAGAGCTTTCTTGGACAACAAGGGAAAGTTCAACGAAGGCCTAAGACAAAACGTGAATGGGCTGATGAGCTTATATGAAGCTTCACACCTATGCATGCATGGCGATGACATACTTGAAGAAGCTGAAACTTTCAGCACCCATGGCCTTAATCATTGCCTTGCTCATCTTGATCATCATATGGCCCCTTTTGTGCGCAACACTTTAGCTCATCCCCACCATAAAAGCGTTGCTAAATTCATGGTGCCTACCTATTTTGGAGACATTCAAAGCACAAACAAATGGATCCATGTTTTGCAAGATGTTGCCAAAATGGATTTCAATGCAGCTCAGCTGCTACACCAAGACGAACTTGTTCAGTTCTTGAAATGGTGGAAAGAAACAGGCTTGGCGAAAGAGTTGAAGTTTGCAAGAGATGAACCTATTAAATGGTACATAGAATCACTAGTTTGTCTCGGAGATTCATGCTATTCCCAACAAAGACTCCAACTTGCAAAGTCCATCTCTTTTGTGTATCTCATTGATGACCTTTATGATGTATTTGGAAATCTTAAAGAACTCACCTTCTTCACCGAAGCTGTCTGCAGATGGGATTTGGGTGCTGCTAAAAGATTGCCTGATTGCATGGGAATTTGTTTAAAATCTCTATTTGAAGTTACCAATGATGTAAGTTCCATGATCTATAAGAAGCATGGCTGGAATCCCATTGGTTTCTTAAGTAGAGCGTGGGGTAAATTATGCAAGGCCTTCTTAGTGGAAACTGAATGGATGAGTTGTGGATATTCACCAAGTGCAGAAGAATATTTGAGGAATGGCATTGTTAGCACTGGCATCCACGTTATTTTAGTACATGTTTTCTTTCTATTAGGCCAACAAATAAGCCAGCAAACTGCAGAGCTTTTGGAAGATGATTTAGACATTATTTCATCTACTGCCATCATACTCCGACTCTGTGACGACATGGGCAGTGCAAAGGACGAGAAGCAAGTGGGGCTAGATGGATCATATGCAAAACACTACATGAATGAGCATCCAAGCATCTCCCATGAAGCCACACACCATCATGTTACGAACAAAATTTCTCAAGCATGGAAGACTCTCAACAAAGAATATCTCTTATCCAACTTATTCCCAGCAAAGTTCACTCAGGCTTCTCTGAACATTGCAAGAGCAGTCCCTTTGGTTTATAATTATGGCTCAAACCAATCATTTATGACACTTGAAAAGCTCATACAACAATTGTTGTTTCGTATATATGTGTAACATTGGATCTTCATACACATTTATTACTACAAACTAAAACAAAACCATGATGAATACTCAAACAAAAATACACATTTATAATTGTTGTGTAATGATACTATCCTAG

Coding sequence (CDS)

ATGGCCAACCTTCTCTTCTTTCTTCCTCCTCCTCCTCCTCCAATAACCTTAACCTTAATGTCTCACTCAAAGATTCCAAAAGCTTTTCACAACCATTCATGGCTTTTCAAGAACCCTATCGAGAAACCAGGTTTTGCAGATAAAAAATGGAGCATTCATGCGGATTCTTCTTCCTTCAATCTCTTAAACTTTGCTGATGGCCTTGGGCTTGTTGAAGCTCCAGCTAAAGTGGATATTTTAAGGCATTTATTGAGAGACCAAACTGGAGATTCCTTGGATTGCTTGAACCTTATTGATGCCACCCAACGCCTTGGCATTGATTACCACTTCCAACAGGAGATTCAAGTTGTTCTTCAAAGGCTATACGTCTTATCTAACCATCAATGGCTAGATCGCGAGACCAATCTTCATTATACTTCTCTTCTCTTTCGACTTTTGAGACAACAAGGTTATCCCGTGTCCACAGATAATTTCAGAGCTTTCTTGGACAACAAGGGAAAGTTCAACGAAGGCCTAAGACAAAACGTGAATGGGCTGATGAGCTTATATGAAGCTTCACACCTATGCATGCATGGCGATGACATACTTGAAGAAGCTGAAACTTTCAGCACCCATGGCCTTAATCATTGCCTTGCTCATCTTGATCATCATATGGCCCCTTTTGTGCGCAACACTTTAGCTCATCCCCACCATAAAAGCGTTGCTAAATTCATGGTGCCTACCTATTTTGGAGACATTCAAAGCACAAACAAATGGATCCATGTTTTGCAAGATGTTGCCAAAATGGATTTCAATGCAGCTCAGCTGCTACACCAAGACGAACTTGTTCAGTTCTTGAAATGGTGGAAAGAAACAGGCTTGGCGAAAGAGTTGAAGTTTGCAAGAGATGAACCTATTAAATGGTACATAGAATCACTAGTTTGTCTCGGAGATTCATGCTATTCCCAACAAAGACTCCAACTTGCAAAGTCCATCTCTTTTGTGTATCTCATTGATGACCTTTATGATGTATTTGGAAATCTTAAAGAACTCACCTTCTTCACCGAAGCTGTCTGCAGATGGGATTTGGGTGCTGCTAAAAGATTGCCTGATTGCATGGGAATTTGTTTAAAATCTCTATTTGAAGTTACCAATGATGTAAGTTCCATGATCTATAAGAAGCATGGCTGGAATCCCATTGGTTTCTTAAGTAGAGCGTGGGGTAAATTATGCAAGGCCTTCTTAGTGGAAACTGAATGGATGAGTTGTGGATATTCACCAAGTGCAGAAGAATATTTGAGGAATGGCATTGTTAGCACTGGCATCCACGTTATTTTAGTACATGTTTTCTTTCTATTAGGCCAACAAATAAGCCAGCAAACTGCAGAGCTTTTGGAAGATGATTTAGACATTATTTCATCTACTGCCATCATACTCCGACTCTGTGACGACATGGGCAGTGCAAAGGACGAGAAGCAAGTGGGGCTAGATGGATCATATGCAAAACACTACATGAATGAGCATCCAAGCATCTCCCATGAAGCCACACACCATCATGTTACGAACAAAATTTCTCAAGCATGGAAGACTCTCAACAAAGAATATCTCTTATCCAACTTATTCCCAGCAAAGTTCACTCAGGCTTCTCTGAACATTGCAAGAGCAGTCCCTTTGGTTTATAATTATGGCTCAAACCAATCATTTATGACACTTGAAAAGCTCATACAACAATTGTTGTTTCGTATATATGTGTAA

Protein sequence

MANLLFFLPPPPPPITLTLMSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFNLLNFADGLGLVEAPAKVDILRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQSFMTLEKLIQQLLFRIYV
Homology
BLAST of CmaCh04G000520 vs. ExPASy Swiss-Prot
Match: P0CV94 ((3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.9e-149
Identity = 264/504 (52.38%), Postives = 352/504 (69.84%), Query Frame = 0

Query: 71  VEAPAKVDILRHLLRDQTG-DSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQW 130
           V+   K+++L+ +LR+    D+L+ LN+IDA QRLGIDY+FQ+EI  +L +   +     
Sbjct: 16  VQHSQKLELLKTVLRNVAELDALEGLNMIDAVQRLGIDYNFQREIDEILHKQMSI----- 75

Query: 131 LDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLC 190
           +    +LH  +L FRLLRQ GY V  D F  F D+KG F + L +++ GLMSLYEAS L 
Sbjct: 76  VSARDDLHEVALRFRLLRQHGYFVPEDVFNNFKDSKGTFKQVLGEDIKGLMSLYEASQLG 135

Query: 191 MHGDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQST 250
             G+DIL EAE FS H L   L+HLDHH    V NTL +PHHKS+A FM   +F   Q+T
Sbjct: 136 TEGEDILVEAEKFSGHLLKTSLSHLDHHRVRIVANTLRNPHHKSLAPFMARNFFVTSQAT 195

Query: 251 NKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCL 310
           N W+++L++VAK DFN  + LHQ+E+VQ  KWWKE GLAKELKFARD+P+KWYI S+ CL
Sbjct: 196 NSWLNLLKEVAKTDFNMVRSLHQNEIVQMSKWWKELGLAKELKFARDQPLKWYIWSMACL 255

Query: 311 GDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGIC 370
            D   S++R++L K ISFVYLIDD++DV+G L +L  FTEAV RW++ A   LPD M IC
Sbjct: 256 TDPKLSEERVELTKPISFVYLIDDIFDVYGTLDDLILFTEAVNRWEITAIDHLPDYMKIC 315

Query: 371 LKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNG 430
            K+L+++TN+ SS +Y KHGWNP+  L  +W  LC AFLVE +W + G  P +EEYL+NG
Sbjct: 316 FKALYDMTNEFSSKVYLKHGWNPLQSLKISWASLCNAFLVEAKWFASGKLPKSEEYLKNG 375

Query: 431 IVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGL 490
           IVS+G++V+LVH+FFLLGQ I++++ ELL +   IISS+A ILRL DD+GSAKDE Q G 
Sbjct: 376 IVSSGVNVVLVHMFFLLGQNITRKSVELLNETPAIISSSAAILRLWDDLGSAKDENQDGN 435

Query: 491 DGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAV 550
           DGSY + Y+ EH   S E       N IS  WK LN+E L  N FPA FT ASLN+AR +
Sbjct: 436 DGSYVRCYLEEHEGCSIEEAREKTINMISDEWKKLNRELLSPNPFPASFTLASLNLARMI 495

Query: 551 PLVYNYGSNQSFMTLEKLIQQLLF 574
           PL+Y+Y  NQ   +L++ ++ +L+
Sbjct: 496 PLMYSYDGNQCLPSLKEYMKLMLY 514

BLAST of CmaCh04G000520 vs. ExPASy Swiss-Prot
Match: P0CV95 ((3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.6e-148
Identity = 261/499 (52.30%), Postives = 350/499 (70.14%), Query Frame = 0

Query: 76  KVDILRHLLRDQTG-DSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRET 135
           K+++ R++LR+    D+L+ LN+IDA QRLGID+HFQ+EI  +L +   +SN   +    
Sbjct: 80  KLELFRNVLRNVAELDALEGLNMIDAVQRLGIDFHFQREIDEILHK--QMSN---VSASD 139

Query: 136 NLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDD 195
           +LH  +L FRLLRQ GY V  D F  F D+KG F + L +++ GLMSLYEAS L   G+D
Sbjct: 140 DLHEVALRFRLLRQHGYFVPEDVFNNFKDSKGTFKQVLGEDIKGLMSLYEASQLGTEGED 199

Query: 196 ILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIH 255
            L EAE FS H L   L+HLDHH A  V NTL +PHHKS+A FM   +F   Q+TN W++
Sbjct: 200 TLVEAEKFSGHLLKTSLSHLDHHHARIVGNTLRNPHHKSLASFMARNFFVTTQATNSWLN 259

Query: 256 VLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCY 315
           +L+DVAK DFN  + LHQ+E+VQ  KWWKE GLAKELKFARD+P KWYI S+ CL D   
Sbjct: 260 LLKDVAKTDFNMVRSLHQNEIVQISKWWKELGLAKELKFARDQPQKWYIWSMACLTDPKL 319

Query: 316 SQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLF 375
           S++R++L K ISFVYLIDD++DV+G L +L  FTEAV RW++ A   LPD M IC K+L+
Sbjct: 320 SEERVELTKPISFVYLIDDIFDVYGTLDDLILFTEAVNRWEITAIDHLPDYMKICFKALY 379

Query: 376 EVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTG 435
           ++TN++S  +Y+KHGWNP+  L  +W  LC AFLVE +W + G  P ++EYL+NGIVS+G
Sbjct: 380 DMTNEISCKVYQKHGWNPLQSLKISWASLCNAFLVEAKWFASGQLPKSKEYLKNGIVSSG 439

Query: 436 IHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYA 495
           ++V+LVH+FF+LGQ I+ ++ ELL +   +ISS+A ILRL DD+GSAKDE Q G DGSY 
Sbjct: 440 VNVVLVHMFFILGQNITTKSVELLNETPAMISSSAAILRLWDDLGSAKDENQDGNDGSYV 499

Query: 496 KHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYN 555
           + Y+ EH   S E       N IS  WK LN+E L  N FPA  T ASLN+AR +PL+Y+
Sbjct: 500 RCYLEEHEGCSIEEAREKTINMISDEWKKLNRELLSPNPFPATITLASLNLARMIPLMYS 559

Query: 556 YGSNQSFMTLEKLIQQLLF 574
           Y  NQ   +L++ ++ +L+
Sbjct: 560 YDGNQCLPSLKEYMKLMLY 573

BLAST of CmaCh04G000520 vs. ExPASy Swiss-Prot
Match: P0CV96 ((3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca OX=57918 PE=1 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 9.3e-144
Identity = 257/504 (50.99%), Postives = 346/504 (68.65%), Query Frame = 0

Query: 71  VEAPAKVDILRHLLRDQTG-DSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQW 130
           V+   K+++ R++LR+    D+L+ LN+IDA QRLGIDYHFQ+EI  +L +   +     
Sbjct: 77  VQHSQKLELFRNVLRNAAELDALEGLNMIDAVQRLGIDYHFQREIDEILHKQMGI----- 136

Query: 131 LDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLC 190
           +    +L+  +L FRLLRQ GY V  D F  F D+KG F + L +++ GLMSLYEAS L 
Sbjct: 137 VSACDDLYEVALRFRLLRQHGYFVPEDVFNNFKDSKGTFKQVLGEDIKGLMSLYEASQLG 196

Query: 191 MHGDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQST 250
             G+D L EAE FS H L   L+HLD H A  V NTL +PH KS+A FM   +F   Q+T
Sbjct: 197 TEGEDTLVEAEKFSGHLLKTSLSHLDRHRARIVGNTLRNPHRKSLASFMARNFFVTSQAT 256

Query: 251 NKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCL 310
           N W+++L++VAK DFN  + +HQ E+VQ  KWWKE GL KELKFARD+P+KWY  S+  L
Sbjct: 257 NSWLNLLKEVAKTDFNMVRSVHQKEIVQISKWWKELGLVKELKFARDQPLKWYTWSMAGL 316

Query: 311 GDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGIC 370
            D   S++R++L K ISFVYLIDD++DV+G L +L  FTEAV RW++ A   LPD M IC
Sbjct: 317 TDPKLSEERVELTKPISFVYLIDDIFDVYGTLDDLILFTEAVNRWEITAIDHLPDYMKIC 376

Query: 371 LKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNG 430
            K+L+++TN+ S  +Y+KHGWNP+  L  +W  LC AFLVE +W + G  P +EEYL+NG
Sbjct: 377 FKALYDMTNEFSCKVYQKHGWNPLRSLKISWASLCNAFLVEAKWFASGQLPKSEEYLKNG 436

Query: 431 IVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGL 490
           IVS+G++V LVH+FFLLGQ I++++ ELL +   +ISS+A ILRL DD+GSAKDE Q G 
Sbjct: 437 IVSSGVNVGLVHMFFLLGQNITRKSVELLNETPAMISSSAAILRLWDDLGSAKDENQDGN 496

Query: 491 DGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAV 550
           DGSY + Y+ EH   S E       N IS  WK LN+E L  N FPA FT ASLN+AR +
Sbjct: 497 DGSYVRCYLEEHEGCSIEEAREKTINMISDEWKKLNRELLSPNPFPATFTSASLNLARMI 556

Query: 551 PLVYNYGSNQSFMTLEKLIQQLLF 574
           PL+Y+Y  NQS  +L++ ++ +L+
Sbjct: 557 PLMYSYDGNQSLPSLKEYMKLMLY 575

BLAST of CmaCh04G000520 vs. ExPASy Swiss-Prot
Match: B9RXW4 (Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.2e-128
Identity = 232/496 (46.77%), Postives = 322/496 (64.92%), Query Frame = 0

Query: 81  RHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYTS 140
           +H+LR +   S + L +IDA QRLGID+HFQ EI  +LQR Y + ++     + +LH  +
Sbjct: 75  KHILRKEGEGSHEVLAMIDAIQRLGIDHHFQDEIDEILQRQYTIPSYY---NDNDLHGLA 134

Query: 141 LLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDD-ILEEA 200
           L FRLLRQ GY VS   F  F D +G F++ L  ++ GLM LYEAS L +  +D IL+EA
Sbjct: 135 LRFRLLRQGGYNVSAGVFDKFKDKEGNFDQKLSDDIRGLMELYEASQLSIGAEDHILDEA 194

Query: 201 ETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTY--FGDIQSTNKWIHVLQ 260
             +S   L+  +  LD   A  ++NTL HPHHK++A+F    +  +  + +   W++ LQ
Sbjct: 195 GDYSHQLLSSWMTRLDDSQARIIKNTLDHPHHKNLARFRATNFNRYFHMANIEGWMNELQ 254

Query: 261 DVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQ 320
           ++AK+DF   Q  +Q E+ Q   WWK+ G++KELKF R++P+KWYI S+  L D   SQQ
Sbjct: 255 ELAKIDFQMVQSQNQQEIFQVAGWWKDLGISKELKFVRNQPLKWYIWSMATLSDPSLSQQ 314

Query: 321 RLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVT 380
           R+ L K ISF+Y+IDD++DV G+L ELT FTE V RWD+ A ++LP  M  C K+L  VT
Sbjct: 315 RIDLTKPISFIYIIDDIFDVQGSLDELTLFTEIVKRWDVEAVEQLPGYMRACFKALDSVT 374

Query: 381 NDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHV 440
           N++   +YK+HGWNP+  L   W  LCKAFLVE  W + G+ P+AEEYL+NGIVS+G+HV
Sbjct: 375 NEIGYKVYKQHGWNPVHSLRETWASLCKAFLVEARWFASGHLPAAEEYLQNGIVSSGVHV 434

Query: 441 ILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHY 500
           +LVH+F+LLG  ++++  + + +   II+STA ILRL DD+G +KDE Q G DGSY + Y
Sbjct: 435 VLVHIFYLLGHGVTREGVDFIGNRPAIITSTATILRLWDDLGISKDENQDGHDGSYVECY 494

Query: 501 MNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGS 560
           + EH     E     VT  IS AWK LN+E L  N F   FT++ LN+AR VPL+Y+Y  
Sbjct: 495 VKEHKGSLVEIATKKVTVMISDAWKQLNQECLHPNPFSPNFTKSCLNLARMVPLMYSYDD 554

Query: 561 NQSFMTLEKLIQQLLF 574
           N     LE   + LLF
Sbjct: 555 NHRLPVLEYYTKSLLF 567

BLAST of CmaCh04G000520 vs. ExPASy Swiss-Prot
Match: B9RHX7 (Probable terpene synthase 4 OS=Ricinus communis OX=3988 GN=TPS4 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 2.1e-119
Identity = 228/485 (47.01%), Postives = 311/485 (64.12%), Query Frame = 0

Query: 95  LNLIDATQRLGIDYHFQQEIQVVLQRLY--VLSNHQWLDRETNLHYTSLLFRLLRQQGYP 154
           L +IDA QRLGIDYHFQ+E Q VL+  Y  + + HQ    + +L   +L FRLLRQQGY 
Sbjct: 78  LIMIDALQRLGIDYHFQEETQDVLEGQYNKIFAAHQ----QHHLSDAALRFRLLRQQGYY 137

Query: 155 V-STDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEAETFSTHGLNHCL 214
           V ++D F    + +GKF + L  ++ GLM LYEAS L +  ++IL+EA  FS H LN   
Sbjct: 138 VPASDVFSELKNREGKFKQELAADIKGLMELYEASQLSIQEENILDEAGAFSAHFLNCWT 197

Query: 215 AHLDHHMAPFVRNTLAHPHHKSVAKFMVPTY--FGDIQSTNKWIHVLQDVAKMDFNAAQL 274
            HL  H    V NTL HP H+++A+  +  +  F + Q  N++I    ++AK+DFN  Q 
Sbjct: 198 PHLCDHQTRIVSNTLKHPFHRTLARSTIKNFLHFYNFQGENEYIQTFTELAKLDFNMIQS 257

Query: 275 LHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRLQLAKSISFVY 334
           +H+ E+ Q   WW   GLA ELKFARD+P KW +  LV + D   S QR++LAK +S VY
Sbjct: 258 IHRQEINQVSNWWNNLGLASELKFARDQPEKWCMWPLVGVTDPSLSWQRIELAKPVSLVY 317

Query: 335 LIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTNDVSSMIYKKHG 394
           LIDD++D+ G   +LT FTEAV RW++ A + LP  M IC ++L++VTN ++  +YKKH 
Sbjct: 318 LIDDIFDLGGTPDQLTLFTEAVNRWEITATEDLPYHMKICFRALYDVTNQIAYKVYKKHQ 377

Query: 395 WNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQ 454
           +NPI  L +AW +LC AFL E +W + G  P A+EYL   IV++G+H++LVH FFL+G  
Sbjct: 378 YNPIHSLKKAWARLCNAFLEEAKWFAAGKLPKADEYLNTAIVTSGVHLVLVHTFFLMGDG 437

Query: 455 ISQQTAELL-EDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMNEHPSISHEA 514
           I+ QT  LL  DD  IISS A ILRL DD+GSA+DE Q G DGSY + YM + P  S   
Sbjct: 438 ITDQTINLLNNDDPGIISSVATILRLWDDLGSAQDENQDGYDGSYIECYMKDFPGTSVRD 497

Query: 515 THHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQSFMTLEKLI 574
             +HV + IS  WK LN+  L  N F   F +A+LN AR VPL+Y++ SN +   L++ I
Sbjct: 498 ARNHVISMISDTWKKLNQHCLSPNPFSGSFIRATLNGARMVPLMYDFDSNHNLPILQQNI 557

BLAST of CmaCh04G000520 vs. ExPASy TrEMBL
Match: A0A6J1JC19 ((3S,6E)-nerolidol synthase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484385 PE=4 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 20  MSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFNLLNFADGLGLVEAPAKVDI 79
           MSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFNLLNFADGLGLVEAPAKVDI
Sbjct: 1   MSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFNLLNFADGLGLVEAPAKVDI 60

Query: 80  LRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYT 139
           LRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYT
Sbjct: 61  LRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYT 120

Query: 140 SLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEA 199
           SLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEA
Sbjct: 121 SLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEA 180

Query: 200 ETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIHVLQDV 259
           ETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIHVLQDV
Sbjct: 181 ETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIHVLQDV 240

Query: 260 AKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRL 319
           AKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRL
Sbjct: 241 AKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRL 300

Query: 320 QLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTND 379
           QLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTND
Sbjct: 301 QLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTND 360

Query: 380 VSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVIL 439
           VSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVIL
Sbjct: 361 VSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVIL 420

Query: 440 VHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMN 499
           VHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMN
Sbjct: 421 VHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMN 480

Query: 500 EHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ 559
           EHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ
Sbjct: 481 EHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ 540

Query: 560 SFMTLEKLIQQLLFRIYV 578
           SFMTLEKLIQQLLFRIYV
Sbjct: 541 SFMTLEKLIQQLLFRIYV 558

BLAST of CmaCh04G000520 vs. ExPASy TrEMBL
Match: A0A6J1FTJ8 ((3S,6E)-nerolidol synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447175 PE=4 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.2e-306
Identity = 523/578 (90.48%), Postives = 541/578 (93.60%), Query Frame = 0

Query: 1   MANLLFFLPPPPPPITLTLMSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFN 60
           MA+LLFFLPPP     +TLMS SKIPKAFHNHS LFKNPIEKPGF DKKWSI ADSSSFN
Sbjct: 1   MASLLFFLPPP-----ITLMSQSKIPKAFHNHSRLFKNPIEKPGFGDKKWSIRADSSSFN 60

Query: 61  LLNFADGLGLVEAPAKVDILRHLLRDQTGD-SLDCLNLIDATQRLGIDYHFQQEIQVVLQ 120
           LLNFADGLGLVEAPAK+DILRHLLR QTGD SL+CL+LIDATQRLGIDYHFQQEIQVVLQ
Sbjct: 61  LLNFADGLGLVEAPAKLDILRHLLRHQTGDYSLECLSLIDATQRLGIDYHFQQEIQVVLQ 120

Query: 121 RLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGL 180
            LYV SNHQ   ++TNLHYT LLFRLLRQQGYPVSTD+F  FLDNKGKFN+GL QNVNGL
Sbjct: 121 SLYVSSNHQ---QDTNLHYTCLLFRLLRQQGYPVSTDSFTIFLDNKGKFNQGLTQNVNGL 180

Query: 181 MSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMV 240
           MSLYEAS LCMHGDDILEEAETFSTHGLNHCLAHLDHHMAP VRNTLAHPHHKSVAKFMV
Sbjct: 181 MSLYEASQLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPLVRNTLAHPHHKSVAKFMV 240

Query: 241 PTYFGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPI 300
           P YFGDIQSTNKWIHVLQDVAK+DFN  QLLHQDELVQFLKWWKETGLA+ELKFARDEPI
Sbjct: 241 PNYFGDIQSTNKWIHVLQDVAKVDFNRTQLLHQDELVQFLKWWKETGLAEELKFARDEPI 300

Query: 301 KWYIESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAA 360
            WYI SLVCLGDSCYSQQRLQL K+ISFV+LIDDL+DVFGNL ELT FTEAV RWDLGAA
Sbjct: 301 IWYIASLVCLGDSCYSQQRLQLGKTISFVFLIDDLFDVFGNLNELTLFTEAVYRWDLGAA 360

Query: 361 KRLPDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS 420
           K LPDCMG+CLKSLFEVTND+SSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS
Sbjct: 361 KTLPDCMGVCLKSLFEVTNDISSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS 420

Query: 421 PSAEEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMG 480
           PSAEEYLRNGIVSTGIHVILVHVFFLLGQQIS QTAELLEDDLDIISSTAIILRLCDDMG
Sbjct: 421 PSAEEYLRNGIVSTGIHVILVHVFFLLGQQISHQTAELLEDDLDIISSTAIILRLCDDMG 480

Query: 481 SAKDEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFT 540
           SAKDEKQVG DGSYAK+YMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFT
Sbjct: 481 SAKDEKQVGRDGSYAKYYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFT 540

Query: 541 QASLNIARAVPLVYNYGSNQSFMTLEKLIQQLLFRIYV 578
           QASLNIARAVPL  NYGSNQSFM LEKLIQQLLFR+YV
Sbjct: 541 QASLNIARAVPLANNYGSNQSFMPLEKLIQQLLFRVYV 570

BLAST of CmaCh04G000520 vs. ExPASy TrEMBL
Match: A0A6J1CEY1 ((3S,6E)-nerolidol synthase 1-like OS=Momordica charantia OX=3673 GN=LOC111010887 PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 2.0e-202
Identity = 349/502 (69.52%), Postives = 410/502 (81.67%), Query Frame = 0

Query: 72  EAPAKVDILRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLD 131
           E PAK+ IL+H+LR + GD  +CLNLIDA QRLGID HFQ+EI+ VLQ  YVL N     
Sbjct: 69  EDPAKLRILKHVLR-EVGDPWECLNLIDAAQRLGIDNHFQEEIEAVLQTQYVLFNAA-SS 128

Query: 132 RETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMH 191
           R  +LH  +LLFRL RQQGY VS   F+ FLD +GKF+E LR+++NGL SLYEAS LCMH
Sbjct: 129 RNNDLHKAALLFRLFRQQGYQVS-PVFKNFLDKEGKFHEKLREDINGLTSLYEASQLCMH 188

Query: 192 GDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNK 251
           GD+ILEEAE FS H LN  +AH+DHH+A FV NTLAHPHHKS+A+FMVP YFGDIQ  NK
Sbjct: 189 GDEILEEAENFSRHWLNARVAHVDHHVATFVHNTLAHPHHKSMAQFMVPNYFGDIQWGNK 248

Query: 252 WIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGD 311
           WI++LQD AK++F A Q LHQ+E+ QFLKWWKET LAKEL FARD+PIKWY+ SLVCL D
Sbjct: 249 WINILQDAAKIEFYATQRLHQNEIAQFLKWWKETDLAKELNFARDQPIKWYMASLVCLTD 308

Query: 312 SCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLK 371
           S +S+QR++LAKSISFVYLIDDL+DVFG   ELT  TEAV RWDL AAK LPDCM ICL+
Sbjct: 309 SYFSEQRVELAKSISFVYLIDDLFDVFGTHDELTLLTEAVHRWDLAAAKGLPDCMRICLR 368

Query: 372 SLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIV 431
           SLFEVTN++S  IY+KHGWNPIG L +AW KLCK FLVE EW+SCG+SPSAEEYLRNGI 
Sbjct: 369 SLFEVTNEISYKIYQKHGWNPIGSLKKAWAKLCKTFLVEAEWLSCGHSPSAEEYLRNGIA 428

Query: 432 STGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDG 491
           STGI VILVHVFFL+GQQIS +T +LLEDDLDI+SS+A +LRL DDMG+A+DEKQ G DG
Sbjct: 429 STGIPVILVHVFFLIGQQISNETVQLLEDDLDIVSSSAAVLRLWDDMGTAQDEKQEGRDG 488

Query: 492 SYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPL 551
           SY + YM EHPS+S E+T  HV  KI +AWKTLN+EYLLS  FP+KFTQASLN+ARAVPL
Sbjct: 489 SYLECYMKEHPSVSCESTQQHVMKKICEAWKTLNREYLLSKTFPSKFTQASLNVARAVPL 548

Query: 552 VYNYGSNQSFMTLEKLIQQLLF 574
            YNYG N+S MT+E +++ L F
Sbjct: 549 AYNYGRNRSIMTMENIVENLFF 567

BLAST of CmaCh04G000520 vs. ExPASy TrEMBL
Match: A0A6J1CE33 ((3S,6E)-nerolidol synthase 1-like OS=Momordica charantia OX=3673 GN=LOC111010706 PE=4 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 7.3e-200
Identity = 353/561 (62.92%), Postives = 427/561 (76.11%), Query Frame = 0

Query: 23  SKIPKAFHN-HSWLFKNPIEKPGFADKKWS--------IHADSSSFNLLNFADGLGLVEA 82
           S  P   HN H     NP+ +P  A+KK +        I  ++ + ++   +D  G+  A
Sbjct: 12  SAAPLNIHNAHHHKPMNPMIRP-LANKKQTIITIPLPLICCNNKNQSVTTLSDDYGVGAA 71

Query: 83  PAKVDILRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRE 142
            AK+ IL+H+LR + GDS +CLNLIDA QRLGIDYHFQ+EI+ VL + Y+L N    D  
Sbjct: 72  TAKLKILKHVLR-EIGDSYECLNLIDAAQRLGIDYHFQEEIESVLHKQYILFNAVQFDPH 131

Query: 143 TNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGD 202
           T+L   SLLFRL RQQGYP S D F+ FLD+ GKF+E LR ++ GL SLYEAS LCMH +
Sbjct: 132 TDLPKASLLFRLFRQQGYPASADVFKIFLDHVGKFDEELRHDIKGLTSLYEASQLCMHEE 191

Query: 203 DILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWI 262
           DILEEAE+FS+H L+    H+DHH A FV NTLAHP HKSVA+FMVP YFGDIQ TNKWI
Sbjct: 192 DILEEAESFSSHWLSAWAVHVDHHSAAFVHNTLAHPCHKSVAQFMVPNYFGDIQWTNKWI 251

Query: 263 HVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSC 322
           H+LQDVA++DFN  Q L Q+E+ QF KWW+ET LAKEL FAR++PIKWY+ SL+CL D C
Sbjct: 252 HILQDVARLDFNTTQRLRQNEIAQFSKWWEETDLAKELSFARNQPIKWYMGSLICLTDPC 311

Query: 323 YSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSL 382
           +S++R+ LAKSI+F+YLIDD++DVFG+  ELT FT+ VCRWDL   K+LP+ M + LKSL
Sbjct: 312 FSEERVLLAKSITFIYLIDDIFDVFGSRDELTLFTQLVCRWDLVGGKKLPNSMQVVLKSL 371

Query: 383 FEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVST 442
           FEVTN++S  IY+KHGWNP   L +AW KLCKAFLVE EW+SCG+SPSAEEYLRNGIVST
Sbjct: 372 FEVTNEISYKIYQKHGWNPASSLKKAWAKLCKAFLVEAEWLSCGHSPSAEEYLRNGIVST 431

Query: 443 GIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSY 502
           G+HV+LVHVFFLLG++IS+QT ELLEDDLDII S+A ILRL DDMG+AKDE Q G DGSY
Sbjct: 432 GVHVVLVHVFFLLGERISKQTVELLEDDLDIILSSATILRLWDDMGNAKDENQEGRDGSY 491

Query: 503 AKHYMNEHPSISHEATHHHVTNKISQAWKTLNKE-YLLSNLFPAKFTQASLNIARAVPLV 562
            K+YM EHPS+    T  H+  KIS+AWKTLNKE  LLS   PAKF QASLNIARAVPL 
Sbjct: 492 EKYYMKEHPSMCCNITRQHMMRKISEAWKTLNKESQLLSTTLPAKFIQASLNIARAVPLT 551

Query: 563 YNYGSNQSFMTLEKLIQQLLF 574
           YNYG NQS  T E+LI+QLLF
Sbjct: 552 YNYGRNQSLPTFEQLIEQLLF 570

BLAST of CmaCh04G000520 vs. ExPASy TrEMBL
Match: A0A6J1EH51 (uncharacterized protein LOC111434068 OS=Cucurbita moschata OX=3662 GN=LOC111434068 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 2.5e-192
Identity = 331/505 (65.54%), Postives = 396/505 (78.42%), Query Frame = 0

Query: 72  EAPAKVDILRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLD 131
           EAP K  IL+H+LR + GD  +CLNLIDATQRLGIDYHFQQEI+ +LQR YVL N   L+
Sbjct: 68  EAPVKGRILKHVLR-EIGDPWECLNLIDATQRLGIDYHFQQEIEAILQRQYVLFNAVQLN 127

Query: 132 RETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMH 191
            +T+LH T+ LFRL RQ GY VS+D F +FLD +GKF E L+ ++ GLMSLYEAS LCMH
Sbjct: 128 SDTDLHKTAFLFRLFRQHGYLVSSDVFESFLDGEGKFKEELKDDIKGLMSLYEASQLCMH 187

Query: 192 GDDILEEAETFSTHGLNHC--LAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQST 251
           GD+ILEEAE FS+H L        +DHH+A FV++TLA+PHHKSV + M P Y  D+Q  
Sbjct: 188 GDEILEEAENFSSHWLKARAEAEQVDHHLASFVQHTLAYPHHKSVVQLMAPNYLEDVQWP 247

Query: 252 NKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCL 311
           NKWI + +D AKM+  +AQ L Q EL QF KWWKET LAK+L F+RD+PIKWY+ SL+CL
Sbjct: 248 NKWISIFRDAAKMELYSAQRLRQHELAQFTKWWKETDLAKDLSFSRDQPIKWYVASLICL 307

Query: 312 G-DSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGI 371
             DS YS+QR+QLAKSISF+YLIDD++DVFG L ELT FTEAVCRWDL AA+ LPDCM I
Sbjct: 308 STDSFYSEQRIQLAKSISFIYLIDDIFDVFGTLDELTIFTEAVCRWDLAAAEGLPDCMQI 367

Query: 372 CLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRN 431
           CL++LFEVTN++S  IY+ HGWNPI  L +AW KLCKAFLVE EWMS G SPSAEEYL+N
Sbjct: 368 CLRTLFEVTNEISCQIYQAHGWNPIHSLHKAWAKLCKAFLVEAEWMSSGQSPSAEEYLKN 427

Query: 432 GIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVG 491
           G+VSTG+HV L HVFFLLG+ IS++T EL ++DLDIISS+A +LRL DDMGSAKDEKQ G
Sbjct: 428 GVVSTGVHVTLTHVFFLLGELISKETVELFDEDLDIISSSATVLRLWDDMGSAKDEKQEG 487

Query: 492 LDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARA 551
            DGSY ++YM EHPS+ +E T  H   +I  AWKTLN E LLSNLFPAKF QA LN+AR 
Sbjct: 488 RDGSYLEYYMKEHPSMCYEETKRHTMKQICNAWKTLNTECLLSNLFPAKFNQACLNLARV 547

Query: 552 VPLVYNYGSNQSFMTLEKLIQQLLF 574
           VP+ YNYG  QS M+LE LI+Q LF
Sbjct: 548 VPIAYNYGRAQSIMSLENLIKQFLF 571

BLAST of CmaCh04G000520 vs. NCBI nr
Match: XP_022986721.1 ((3S,6E)-nerolidol synthase 1-like [Cucurbita maxima])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 20  MSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFNLLNFADGLGLVEAPAKVDI 79
           MSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFNLLNFADGLGLVEAPAKVDI
Sbjct: 1   MSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFNLLNFADGLGLVEAPAKVDI 60

Query: 80  LRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYT 139
           LRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYT
Sbjct: 61  LRHLLRDQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYT 120

Query: 140 SLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEA 199
           SLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEA
Sbjct: 121 SLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEA 180

Query: 200 ETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIHVLQDV 259
           ETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIHVLQDV
Sbjct: 181 ETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNKWIHVLQDV 240

Query: 260 AKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRL 319
           AKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRL
Sbjct: 241 AKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRL 300

Query: 320 QLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTND 379
           QLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTND
Sbjct: 301 QLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTND 360

Query: 380 VSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVIL 439
           VSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVIL
Sbjct: 361 VSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVIL 420

Query: 440 VHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMN 499
           VHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMN
Sbjct: 421 VHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMN 480

Query: 500 EHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ 559
           EHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ
Sbjct: 481 EHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ 540

Query: 560 SFMTLEKLIQQLLFRIYV 578
           SFMTLEKLIQQLLFRIYV
Sbjct: 541 SFMTLEKLIQQLLFRIYV 558

BLAST of CmaCh04G000520 vs. NCBI nr
Match: KAG6599894.1 (hypothetical protein SDJN03_05127, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 531/585 (90.77%), Postives = 549/585 (93.85%), Query Frame = 0

Query: 1   MANLLFFLPPPPPPITLTLMSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFN 60
           MA+LLFFLPPPPPPI  TLMS SKIPK FHNHSWLFKNPIEKPGF DKKWSIHADSSSFN
Sbjct: 1   MASLLFFLPPPPPPI--TLMSQSKIPKPFHNHSWLFKNPIEKPGFGDKKWSIHADSSSFN 60

Query: 61  LLNFADGLGLVEAPAKVDILRHLLRDQTGD-SLDCLNLIDATQRLGIDYHFQQEIQVVLQ 120
           LLNFADGLGLVEAPAK+DILRHLLR QTGD SL+CL+LIDATQRLGIDYHFQQEIQVVLQ
Sbjct: 61  LLNFADGLGLVEAPAKLDILRHLLRHQTGDYSLECLSLIDATQRLGIDYHFQQEIQVVLQ 120

Query: 121 RLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGL 180
            LYV SNHQ   ++TNLHYTSLLFRLLRQQGYPVST++F  FLDNKGKFN+GL QNVNGL
Sbjct: 121 SLYVSSNHQ---QDTNLHYTSLLFRLLRQQGYPVSTESFTIFLDNKGKFNQGLTQNVNGL 180

Query: 181 MSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMV 240
           MSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAP VRNTLAHPHHKSVAKFMV
Sbjct: 181 MSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPLVRNTLAHPHHKSVAKFMV 240

Query: 241 PTYFGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPI 300
           P YFGDIQSTNKWIHVLQDVAK+DFN  QLLHQDELVQFLKWWKETGLA+ELKFARDEPI
Sbjct: 241 PNYFGDIQSTNKWIHVLQDVAKVDFNRTQLLHQDELVQFLKWWKETGLAEELKFARDEPI 300

Query: 301 KWYIESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAA 360
            WYI SLVCLGDSCYSQQRLQL K+ISFV+LIDDL+DVFGNL ELT FTEAVCRWDLGAA
Sbjct: 301 IWYIASLVCLGDSCYSQQRLQLGKTISFVFLIDDLFDVFGNLNELTLFTEAVCRWDLGAA 360

Query: 361 KRLPDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS 420
           K LPDCMG+CLKSLFEVTND+SSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS
Sbjct: 361 KTLPDCMGVCLKSLFEVTNDISSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS 420

Query: 421 PSAEEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMG 480
           PSAEEYLRNGIVSTGIHVILVHVFFLLGQQIS QTAELLEDDLDIISSTAIILRLCDDMG
Sbjct: 421 PSAEEYLRNGIVSTGIHVILVHVFFLLGQQISHQTAELLEDDLDIISSTAIILRLCDDMG 480

Query: 481 SAK-------DEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSN 540
           SAK       DEKQVG DGSYAK+YMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSN
Sbjct: 481 SAKHAFTRAQDEKQVGRDGSYAKYYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSN 540

Query: 541 LFPAKFTQASLNIARAVPLVYNYGSNQSFMTLEKLIQQLLFRIYV 578
           LFPAKFTQASLNIARAVPL YNYGSNQSFM LEKLIQQLLFR+YV
Sbjct: 541 LFPAKFTQASLNIARAVPLAYNYGSNQSFMPLEKLIQQLLFRVYV 580

BLAST of CmaCh04G000520 vs. NCBI nr
Match: XP_022941968.1 ((3S,6E)-nerolidol synthase 1-like [Cucurbita moschata])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.4e-306
Identity = 523/578 (90.48%), Postives = 541/578 (93.60%), Query Frame = 0

Query: 1   MANLLFFLPPPPPPITLTLMSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHADSSSFN 60
           MA+LLFFLPPP     +TLMS SKIPKAFHNHS LFKNPIEKPGF DKKWSI ADSSSFN
Sbjct: 1   MASLLFFLPPP-----ITLMSQSKIPKAFHNHSRLFKNPIEKPGFGDKKWSIRADSSSFN 60

Query: 61  LLNFADGLGLVEAPAKVDILRHLLRDQTGD-SLDCLNLIDATQRLGIDYHFQQEIQVVLQ 120
           LLNFADGLGLVEAPAK+DILRHLLR QTGD SL+CL+LIDATQRLGIDYHFQQEIQVVLQ
Sbjct: 61  LLNFADGLGLVEAPAKLDILRHLLRHQTGDYSLECLSLIDATQRLGIDYHFQQEIQVVLQ 120

Query: 121 RLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGL 180
            LYV SNHQ   ++TNLHYT LLFRLLRQQGYPVSTD+F  FLDNKGKFN+GL QNVNGL
Sbjct: 121 SLYVSSNHQ---QDTNLHYTCLLFRLLRQQGYPVSTDSFTIFLDNKGKFNQGLTQNVNGL 180

Query: 181 MSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPHHKSVAKFMV 240
           MSLYEAS LCMHGDDILEEAETFSTHGLNHCLAHLDHHMAP VRNTLAHPHHKSVAKFMV
Sbjct: 181 MSLYEASQLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPLVRNTLAHPHHKSVAKFMV 240

Query: 241 PTYFGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPI 300
           P YFGDIQSTNKWIHVLQDVAK+DFN  QLLHQDELVQFLKWWKETGLA+ELKFARDEPI
Sbjct: 241 PNYFGDIQSTNKWIHVLQDVAKVDFNRTQLLHQDELVQFLKWWKETGLAEELKFARDEPI 300

Query: 301 KWYIESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAA 360
            WYI SLVCLGDSCYSQQRLQL K+ISFV+LIDDL+DVFGNL ELT FTEAV RWDLGAA
Sbjct: 301 IWYIASLVCLGDSCYSQQRLQLGKTISFVFLIDDLFDVFGNLNELTLFTEAVYRWDLGAA 360

Query: 361 KRLPDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS 420
           K LPDCMG+CLKSLFEVTND+SSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS
Sbjct: 361 KTLPDCMGVCLKSLFEVTNDISSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYS 420

Query: 421 PSAEEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDMG 480
           PSAEEYLRNGIVSTGIHVILVHVFFLLGQQIS QTAELLEDDLDIISSTAIILRLCDDMG
Sbjct: 421 PSAEEYLRNGIVSTGIHVILVHVFFLLGQQISHQTAELLEDDLDIISSTAIILRLCDDMG 480

Query: 481 SAKDEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFT 540
           SAKDEKQVG DGSYAK+YMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFT
Sbjct: 481 SAKDEKQVGRDGSYAKYYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFT 540

Query: 541 QASLNIARAVPLVYNYGSNQSFMTLEKLIQQLLFRIYV 578
           QASLNIARAVPL  NYGSNQSFM LEKLIQQLLFR+YV
Sbjct: 541 QASLNIARAVPLANNYGSNQSFMPLEKLIQQLLFRVYV 570

BLAST of CmaCh04G000520 vs. NCBI nr
Match: KAG7030575.1 (hypothetical protein SDJN02_04612, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 952.6 bits (2461), Expect = 1.6e-273
Identity = 470/527 (89.18%), Postives = 490/527 (92.98%), Query Frame = 0

Query: 52  IHADSSSFNLLNFADGLGLVEAPAKVDILRHLLRDQTGD-SLDCLNLIDATQRLGIDYHF 111
           + + SSS N LN  DGLGLVEAPAK+DILRHLLR QTGD SL+CL+LIDATQRLGIDYHF
Sbjct: 15  LSSSSSSSNNLN--DGLGLVEAPAKLDILRHLLRHQTGDYSLECLSLIDATQRLGIDYHF 74

Query: 112 QQEIQVVLQRLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNE 171
           QQEIQVVLQ LYV SNHQ   ++TNLHYTSLLFRLLRQQGYPVST++F  FLDNKGKFN+
Sbjct: 75  QQEIQVVLQSLYVSSNHQ---QDTNLHYTSLLFRLLRQQGYPVSTESFTIFLDNKGKFNQ 134

Query: 172 GLRQNVNGLMSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPFVRNTLAHPH 231
           GL QNVNGLMSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAP VRNTLAHPH
Sbjct: 135 GLTQNVNGLMSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHLDHHMAPLVRNTLAHPH 194

Query: 232 HKSVAKFMVPTYFGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKE 291
           HKSVAKFMVP YFGDIQSTNKWIHVLQDVAK+DFN  QLLHQDELVQFLKWWKETGLA+E
Sbjct: 195 HKSVAKFMVPNYFGDIQSTNKWIHVLQDVAKVDFNRTQLLHQDELVQFLKWWKETGLAEE 254

Query: 292 LKFARDEPIKWYIESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEA 351
           LKFARDEPI WYI SLVCLGDSCYSQQRLQL K+ISFV+LIDDL+DVFGNL ELT FTEA
Sbjct: 255 LKFARDEPIIWYIASLVCLGDSCYSQQRLQLGKTISFVFLIDDLFDVFGNLNELTLFTEA 314

Query: 352 VCRWDLGAAKRLPDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVE 411
           VCRWDLGAAK LPDCMG+CLKSLFEVTND+SSMIYKKHGWNPIGFLSRAWGKLCKAFLVE
Sbjct: 315 VCRWDLGAAKTLPDCMGVCLKSLFEVTNDISSMIYKKHGWNPIGFLSRAWGKLCKAFLVE 374

Query: 412 TEWMSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAI 471
           TEWMSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQIS QTAELLEDDLDIISSTAI
Sbjct: 375 TEWMSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQISHQTAELLEDDLDIISSTAI 434

Query: 472 ILRLCDDMGSAKDEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLL 531
           ILRLCDDMG       +G DGSYAK+YMNEHPSISHEATHHHVTNKISQAWKTLNKEYLL
Sbjct: 435 ILRLCDDMG-------MGRDGSYAKYYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLL 494

Query: 532 SNLFPAKFTQASLNIARAVPLVYNYGSNQSFMTLEKLIQQLLFRIYV 578
           SNLFPAKFTQASLNIARAVPL YNYGSNQSFM LEKLIQQLLFR+YV
Sbjct: 495 SNLFPAKFTQASLNIARAVPLAYNYGSNQSFMPLEKLIQQLLFRVYV 529

BLAST of CmaCh04G000520 vs. NCBI nr
Match: XP_038891600.1 ((3S,6E)-nerolidol synthase 1-like [Benincasa hispida])

HSP 1 Score: 803.5 bits (2074), Expect = 1.2e-228
Identity = 409/580 (70.52%), Postives = 468/580 (80.69%), Query Frame = 0

Query: 1   MANLLFFLPPPPPPITLTLMSHSKIPKAFHNHSWLFKNPIEKPGFADKKWSIHA---DSS 60
           MA L++  P  P PI   LMS +KIP+ FHN++ L  N         KKWSIHA    +S
Sbjct: 1   MAQLIY--PHCPKPI---LMSQTKIPRVFHNNTLLLNN--------HKKWSIHALDFSTS 60

Query: 61  SFNLLNFADGLGLVEAPAK-VDILRHLLRD-QTGDSLDCLNLIDATQRLGIDYHFQQEIQ 120
           S     F DG G+  A  K V+ILR +LRD Q G+SL+ L+LIDATQRLGIDYHF +EI+
Sbjct: 61  SLKHSYFVDGFGVEVAKTKVVNILRQVLRDNQIGESLEYLDLIDATQRLGIDYHFHEEIE 120

Query: 121 VVLQRLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQN 180
           + L+RLYVLSN Q LD   NLH  SLLFRLLRQQGY +STD F+ FLDNKG FNE LR++
Sbjct: 121 IFLRRLYVLSNAQILDPHNNLHKASLLFRLLRQQGYHMSTDVFKVFLDNKGNFNEELRKD 180

Query: 181 VNGLMSLYEASHLCMHGDD-ILEEAETFSTHGLNHCL-AHLDHHMAPFVRNTLAHPHHKS 240
           + GL SLYEAS +CMHGDD +L EAE FS HGLN  L  HLDHH+A FV NTLAHPHHKS
Sbjct: 181 IKGLSSLYEASQVCMHGDDNVLVEAENFSRHGLNIWLQTHLDHHLANFVHNTLAHPHHKS 240

Query: 241 VAKFMVPTYFGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKF 300
           VAKFMV  YFGD Q TNKWI+VLQDVA+MDFNA Q LHQDE++QFLKWWK+T LAKELKF
Sbjct: 241 VAKFMVGAYFGDTQWTNKWIYVLQDVARMDFNATQKLHQDEILQFLKWWKQTNLAKELKF 300

Query: 301 ARDEPIKWYIESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCR 360
           ARD+P KWY+ SLVCL DSCYSQQRLQLAKSISFVYLIDDL+DVFG+  +LTFFTEAV R
Sbjct: 301 ARDQPTKWYLCSLVCLTDSCYSQQRLQLAKSISFVYLIDDLFDVFGSFNDLTFFTEAVSR 360

Query: 361 WDLGAAKRLPDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEW 420
           WDLGAAK LP+CM IC +SLFEVTN++S+ IYKKHGWNPIG LS+AW KLCKAFLVE EW
Sbjct: 361 WDLGAAKGLPNCMAICFQSLFEVTNEISNKIYKKHGWNPIGLLSKAWAKLCKAFLVEAEW 420

Query: 421 MSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILR 480
           M   +SP AEEYLRNGIVSTGIHVIL+HVFFLL  +IS+QT E+L+DDLDIISS+A+ILR
Sbjct: 421 MRSEHSPRAEEYLRNGIVSTGIHVILIHVFFLLEHKISKQTVEMLDDDLDIISSSAMILR 480

Query: 481 LCDDMGSAKDEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNL 540
           L DDMGSAKDE Q G DGSY K+YMNEHPSIS+EAT HH+  +IS AWKTLN+EYLLSN 
Sbjct: 481 LWDDMGSAKDENQEGHDGSYLKYYMNEHPSISYEATEHHIMERISHAWKTLNREYLLSNT 540

Query: 541 FPAKFTQASLNIARAVPLVYNYGSNQSFMTLEKLIQQLLF 574
           F  +FTQASLN+ARAVPL YNYG NQS +TLEKL++QLLF
Sbjct: 541 FSTRFTQASLNVARAVPLAYNYGRNQSLVTLEKLMEQLLF 567

BLAST of CmaCh04G000520 vs. TAIR 10
Match: AT1G61680.1 (terpene synthase 14 )

HSP 1 Score: 397.9 bits (1021), Expect = 1.4e-110
Identity = 202/469 (43.07%), Postives = 291/469 (62.05%), Query Frame = 0

Query: 95  LNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVS 154
           L +ID  Q LGID HF+QEI+  L  +Y     + L    +LH  +L FRLLRQ+G+ V 
Sbjct: 94  LEMIDVIQSLGIDLHFRQEIEQTLHMIY----KEGLQFNGDLHEIALRFRLLRQEGHYVQ 153

Query: 155 TDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHL 214
              F+  LD KG F + ++ +V GL  L+EAS L + G++ L+ A  F+   LN   +  
Sbjct: 154 EIIFKNILDKKGGFKDVVKNDVKGLTELFEASELRVEGEETLDGAREFTYSRLNELCSGR 213

Query: 215 DHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDI----QSTNKWIHVLQDVAKMDFNAAQLL 274
           + H    +  +LA P HK+V       +   I    Q   +W+  L  VA++D    + L
Sbjct: 214 ESHQKQEIMKSLAQPRHKTVRGLTSKRFTSMIKIAGQEDPEWLQSLLRVAEIDSIRLKSL 273

Query: 275 HQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRLQLAKSISFVYL 334
            Q E+ Q  KWW E GL K+++ AR +P+KW+  S+  L D   ++QRL L K IS VY+
Sbjct: 274 TQGEMSQTFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYV 333

Query: 335 IDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTNDVSSMIYKKHGW 394
           IDD++DV+G L+ELT FT  V RWD    K LP  M +C ++L  +T ++S  IYK HGW
Sbjct: 334 IDDIFDVYGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGW 393

Query: 395 NPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQI 454
           NP   L ++W  LCKAFLVE +W + GY P+ EEY++NG+VS+G+H++++H + LLG+++
Sbjct: 394 NPTYALRQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEEL 453

Query: 455 SQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMNEHPSISHEATH 514
           +++  EL+E +  I+SS A ILRL DD+GSAKDE Q G DGSY + Y+NE+   + +   
Sbjct: 454 TKEKVELIESNPGIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLNEYKGSTVDEAR 513

Query: 515 HHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ 560
            HV  KIS+AWK LN+E L    F   F++A LNIAR VPL+Y+Y  +Q
Sbjct: 514 THVAQKISRAWKRLNRECLNPCPFSRSFSKACLNIARTVPLMYSYDDDQ 558

BLAST of CmaCh04G000520 vs. TAIR 10
Match: AT1G61680.2 (terpene synthase 14 )

HSP 1 Score: 387.5 bits (994), Expect = 1.9e-107
Identity = 199/469 (42.43%), Postives = 288/469 (61.41%), Query Frame = 0

Query: 95  LNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQWLDRETNLHYTSLLFRLLRQQGYPVS 154
           L +ID  Q LGID HF+QEI+  L  +Y     + L    +LH  +L FRLLRQ+G+ V 
Sbjct: 94  LEMIDVIQSLGIDLHFRQEIEQTLHMIY----KEGLQFNGDLHEIALRFRLLRQEGHYVQ 153

Query: 155 TDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDILEEAETFSTHGLNHCLAHL 214
                   + KG F + ++ +V GL  L+EAS L + G++ L+ A  F+   LN   +  
Sbjct: 154 E-------NKKGGFKDVVKNDVKGLTELFEASELRVEGEETLDGAREFTYSRLNELCSGR 213

Query: 215 DHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDI----QSTNKWIHVLQDVAKMDFNAAQLL 274
           + H    +  +LA P HK+V       +   I    Q   +W+  L  VA++D    + L
Sbjct: 214 ESHQKQEIMKSLAQPRHKTVRGLTSKRFTSMIKIAGQEDPEWLQSLLRVAEIDSIRLKSL 273

Query: 275 HQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGDSCYSQQRLQLAKSISFVYL 334
            Q E+ Q  KWW E GL K+++ AR +P+KW+  S+  L D   ++QRL L K IS VY+
Sbjct: 274 TQGEMSQTFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYV 333

Query: 335 IDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLKSLFEVTNDVSSMIYKKHGW 394
           IDD++DV+G L+ELT FT  V RWD    K LP  M +C ++L  +T ++S  IYK HGW
Sbjct: 334 IDDIFDVYGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGW 393

Query: 395 NPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIVSTGIHVILVHVFFLLGQQI 454
           NP   L ++W  LCKAFLVE +W + GY P+ EEY++NG+VS+G+H++++H + LLG+++
Sbjct: 394 NPTYALRQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEEL 453

Query: 455 SQQTAELLEDDLDIISSTAIILRLCDDMGSAKDEKQVGLDGSYAKHYMNEHPSISHEATH 514
           +++  EL+E +  I+SS A ILRL DD+GSAKDE Q G DGSY + Y+NE+   + +   
Sbjct: 454 TKEKVELIESNPGIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLNEYKGSTVDEAR 513

Query: 515 HHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVPLVYNYGSNQ 560
            HV  KIS+AWK LN+E L    F   F++A LNIAR VPL+Y+Y  +Q
Sbjct: 514 THVAQKISRAWKRLNRECLNPCPFSRSFSKACLNIARTVPLMYSYDDDQ 551

BLAST of CmaCh04G000520 vs. TAIR 10
Match: AT4G16730.1 (terpene synthase 02 )

HSP 1 Score: 284.6 bits (727), Expect = 1.7e-76
Identity = 165/520 (31.73%), Postives = 269/520 (51.73%), Query Frame = 0

Query: 71  VEAPAKVDILRHLLR---DQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNH 130
           VE   K  + +  +R   ++   S++ L +ID+ QRLGI YH++ EI  +L++++    H
Sbjct: 18  VETAEKAILFKEEVRKTLNEIEGSIEQLEMIDSLQRLGISYHYKHEIHDILRKIH--DQH 77

Query: 131 QWLDRET-NLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEAS 190
             ++RET +LH TSL F LLRQ G+ VS D F  F+   G+F + L  ++ GL+SLYEAS
Sbjct: 78  GEIERETQDLHATSLEFILLRQHGFDVSQDAFDVFISETGEFRKTLHSDIKGLLSLYEAS 137

Query: 191 HLCMHGDDILEEAETFSTHGLNHCLAHL--------DHHMAPFVRNTLAHPHHKSVAKFM 250
           +  M  +  L+E   ++   L+  +A          + ++   V+  L  P+H S+ +  
Sbjct: 138 YFSMDSEFKLKETRIYANKRLSEFVAESSKTICREDETYILEMVKRALETPYHWSIRRLE 197

Query: 251 VPTYFGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEP 310
              Y    +  ++   +L + A +DFN  Q  HQ+EL     WW  TGL K+L F RD  
Sbjct: 198 ARWYINVYEKKHEMNPLLLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRI 257

Query: 311 IKWYIESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGA 370
            + Y  ++    +  +   R  L K    + ++DD+YD++G L+EL  FT  V +WD+  
Sbjct: 258 TESYFWTIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVNH 317

Query: 371 AKRLPDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGY 430
            +RLP+ M +C   L+   N +   + +  G N I +L + W  L K FL E++W   G+
Sbjct: 318 VERLPNYMRMCFLFLYNEINQIGYDVLRDKGLNVIPYLKQVWTDLFKTFLTESKWYKTGH 377

Query: 431 SPSAEEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAELLEDDLDIISSTAIILRLCDDM 490
            PS EEY++NG++S+ +  IL+H+F +L   IS QT      +  ++ S A ILRL +D+
Sbjct: 378 KPSFEEYMQNGVISSSVPTILLHLFSVLSDHISDQTLTDDSKNHSVVRSCATILRLANDL 437

Query: 491 GSAKDEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNL--FPA 550
            ++ +E   G      + YM E    S E    H+ + IS +W  +N +   ++    P 
Sbjct: 438 ATSTEEMARGDSPKSVQCYMYE-TRASEEEARRHMQSMISDSWDIINSDLKTAHTSSLPR 497

Query: 551 KFTQASLNIARAVPLVYNYGSNQSFMTLEK---LIQQLLF 574
            F  A+ N+ R V  +Y +G         K    IQ +LF
Sbjct: 498 GFLAAAANLNRVVQCIYRHGDGHGSPEKTKTVDYIQSVLF 534

BLAST of CmaCh04G000520 vs. TAIR 10
Match: AT3G25810.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.7e-68
Identity = 158/514 (30.74%), Postives = 256/514 (49.81%), Query Frame = 0

Query: 79  ILRHLLRD---------QTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLSNHQ- 138
           I RH+L+          +T   L+ L LID  Q+LG+ YHF+QEI  +L   ++ +    
Sbjct: 76  ITRHVLKKKVKKMLEEVETKSRLEKLELIDDLQKLGVSYHFEQEINNILTNFHLENGKNI 135

Query: 139 W-LDRETNLHYTSLLFRLLRQQGYPVSTDNFRAFLDNKGKFNEGLRQNVNGLMSLYEASH 198
           W  D+E +LH T+L FRLLRQ G+ VS D F   +D K + N     N+  +++LYEAS+
Sbjct: 136 WKCDKEEDLHATALEFRLLRQHGFGVSEDIFDVIID-KIESNTFKSDNITSIITLYEASY 195

Query: 199 LCMHGDDILEEA-ETFSTHGLNHCLAHLDHHMAPFVRNTLAH----PHHKSVAKFMVPTY 258
           L    D  L +    F+T  + + +          +R    H    P+H  + +     Y
Sbjct: 196 LSTKSDTKLHKVIRPFATEQIRNFVDDESETYNIMLREMAIHALEIPYHWRMRRLETRWY 255

Query: 259 FGDIQSTNKWIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWY 318
               +  +     L + AK+DFN  Q  HQ+++     WWKETGL  +L F RD  ++ Y
Sbjct: 256 IDAYEKKHDMNLFLAEFAKIDFNIVQTAHQEDVKYVSCWWKETGLGSQLHFVRDRIVENY 315

Query: 319 IESLVCLGDSCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRL 378
             ++  + +  +   R  +A   + + +IDD+YD++G  +EL  FT  V  WD+     L
Sbjct: 316 FWTVGMIYEPQFGYIRRIVAIVAALITVIDDIYDIYGTPEELELFTAMVQNWDINRLDEL 375

Query: 379 PDCMGICLKSLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSA 438
           P+ M +C  +LF   N +   + K    + I +  ++W  LCKA+LVE +W   GY PS 
Sbjct: 376 PEYMKLCFLTLFNEINAMGCDVLKCKNIDVIPYFKKSWADLCKAYLVEAKWYKGGYKPSV 435

Query: 439 EEYLRNGIVSTGIHVILVHVFFLLGQQISQQTAE-LLEDDLDIISSTAIILRLCDDMGSA 498
           EEY++N  +S     +L+H +     QIS Q  E L++   D++  +A +LRL +D+ ++
Sbjct: 436 EEYMQNAWISISAPTMLIHFYCAFSGQISVQILESLVQQQQDVVRCSATVLRLANDLATS 495

Query: 499 KDEKQVGLDGSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLL----SNLFPAK 558
            DE   G      + YM+E   +S E    HV   IS  W  +N E       S+L   +
Sbjct: 496 PDELARGDVLKSVQCYMHE-TGVSEEEARTHVQQMISHTWDEMNYEARTAARSSSLLSRR 555

Query: 559 FTQASLNIARAVPLVYNYGSNQSFMTLEKLIQQL 572
           F + ++N+AR    +Y +G         K++ ++
Sbjct: 556 FVETAMNLARMSQCMYQHGDGHGCPDKAKIVDRV 587

BLAST of CmaCh04G000520 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 257.7 bits (657), Expect = 2.2e-68
Identity = 157/486 (32.30%), Postives = 249/486 (51.23%), Query Frame = 0

Query: 86  DQTGDSLDCLNLIDATQRLGIDYHFQQEIQVVLQRLYVLS-----NHQWLDRETNLHYTS 145
           ++T   L+ L LID  QRLG+ YHF+QEI+  L  ++V +     N    +R  +L+ T+
Sbjct: 69  NETEGLLEQLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATA 128

Query: 146 LLFRLLRQQGYPVSTD----NFRAFLDNKGKFNEGLRQNVNGLMSLYEASHLCMHGDDIL 205
           L FRLLRQ G+ ++ D    N    LD+K         ++ G++SLYEAS+L    D  L
Sbjct: 129 LEFRLLRQHGFSIAQDVFDGNIGVDLDDK---------DIKGILSLYEASYLSTRIDTKL 188

Query: 206 EEAETFSTHGLNHCL-----AHLDHHMAPFVRNTLAHPHHKSVAKFMVPTYFGDIQSTNK 265
           +E+  ++T  L   +         + +   V + L  P+H+ V +     Y       + 
Sbjct: 189 KESIYYTTKRLRKFVEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVYGERHD 248

Query: 266 WIHVLQDVAKMDFNAAQLLHQDELVQFLKWWKETGLAKELKFARDEPIKWYIESLVCLGD 325
              +L ++AK+DFN  Q +HQDEL     WW +TGL K L F RD   + Y  S+  + +
Sbjct: 249 MNPILLELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFSSVGVMYE 308

Query: 326 SCYSQQRLQLAKSISFVYLIDDLYDVFGNLKELTFFTEAVCRWDLGAAKRLPDCMGICLK 385
             ++  R  L K    +  IDD+YD++G L+EL  FT  V +WD+   + LP+ M +C  
Sbjct: 309 PEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDVNRLEELPNYMKLCFL 368

Query: 386 SLFEVTNDVSSMIYKKHGWNPIGFLSRAWGKLCKAFLVETEWMSCGYSPSAEEYLRNGIV 445
            L    N +   + +  G+N I +L  +W  +C  FL E +W   GY P+ EEY++NG +
Sbjct: 369 CLVNEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFEEYMQNGWI 428

Query: 446 STGIHVILVHVFFLLGQQISQQTAELLED-DLDIISSTAIILRLCDDMGSAKDEKQVGLD 505
           S+ +  IL+H+F LL    S QT ++L   +  ++ S+A ILRL +D+ ++ +E   G  
Sbjct: 429 SSSVPTILLHLFCLL----SDQTLDILGSYNHSVVRSSATILRLANDLATSSEELARGDT 488

Query: 506 GSYAKHYMNEHPSISHEATHHHVTNKISQAWKTLNKEYLLSNLFPAKFTQASLNIARAVP 557
               + +M+E    S   +  ++   I  AW  LN E     L    F +A+ N+ R   
Sbjct: 489 MKSVQCHMHE-TGASEAESRAYIQGIIGVAWDDLNMEKKSCRLHQG-FLEAAANLGRVAQ 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0CV941.9e-14952.38(3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1[more]
P0CV953.6e-14852.30(3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa O... [more]
P0CV969.3e-14450.99(3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca OX=57918 PE=1 SV=1[more]
B9RXW43.2e-12846.77Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1[more]
B9RHX72.1e-11947.01Probable terpene synthase 4 OS=Ricinus communis OX=3988 GN=TPS4 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JC190.0e+00100.00(3S,6E)-nerolidol synthase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484385 PE... [more]
A0A6J1FTJ81.2e-30690.48(3S,6E)-nerolidol synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447175 ... [more]
A0A6J1CEY12.0e-20269.52(3S,6E)-nerolidol synthase 1-like OS=Momordica charantia OX=3673 GN=LOC111010887... [more]
A0A6J1CE337.3e-20062.92(3S,6E)-nerolidol synthase 1-like OS=Momordica charantia OX=3673 GN=LOC111010706... [more]
A0A6J1EH512.5e-19265.54uncharacterized protein LOC111434068 OS=Cucurbita moschata OX=3662 GN=LOC1114340... [more]
Match NameE-valueIdentityDescription
XP_022986721.10.0e+00100.00(3S,6E)-nerolidol synthase 1-like [Cucurbita maxima][more]
KAG6599894.10.0e+0090.77hypothetical protein SDJN03_05127, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022941968.12.4e-30690.48(3S,6E)-nerolidol synthase 1-like [Cucurbita moschata][more]
KAG7030575.11.6e-27389.18hypothetical protein SDJN02_04612, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_038891600.11.2e-22870.52(3S,6E)-nerolidol synthase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G61680.11.4e-11043.07terpene synthase 14 [more]
AT1G61680.21.9e-10742.43terpene synthase 14 [more]
AT4G16730.11.7e-7631.73terpene synthase 02 [more]
AT3G25810.11.7e-6830.74Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT4G16740.12.2e-6832.30terpene synthase 03 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 267..576
e-value: 5.8E-99
score: 333.0
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 255..561
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 75..266
e-value: 1.1E-55
score: 190.2
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 259..523
e-value: 9.4E-75
score: 251.4
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 87..220
e-value: 2.4E-41
score: 141.6
NoneNo IPR availableSFLDSFLDS00005Isoprenoid_Synthase_Type_Icoord: 258..575
e-value: 0.0
score: 269.1
NoneNo IPR availablePANTHERPTHR31225:SF148(3S,6E)-NEROLIDOL SYNTHASE 2, CHLOROPLASTIC/MITOCHONDRIAL-LIKEcoord: 55..571
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 55..571
IPR034741Terpene cyclase-like 1, C-terminal domainSFLDSFLDG01019Terpene_Cyclase_Like_1_C_Termcoord: 258..575
e-value: 0.0
score: 269.1
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 80..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G000520.1CmaCh04G000520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity