CmaCh04G000250 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G000250
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Location	Cma_Chr04: 124178 .. 126467 (+)
RNA-Seq Expression	CmaCh04G000250
Synteny	CmaCh04G000250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAACTGGAAGACCGTAATTTCTGCTTCATTTTCTGGGCTTCTCACAAATGAACATTTTGAAGCATCCAATTATCAGCAGGCGAGGATTCAGATATGGTGGAGGTACTGTATGTTCATTTCTAATTTTGAGACTCTGGTTTTTGGATTCTCGTTCAAATGTTAGGCGCACATTCGTTTCTGTTCCCCCTTTTTCTTTTCTGCTTACCTCATGTATTTGCTTGATCAACTTTTTTGGATTACAATGGTTTCAAAATAGAGTGAGGGGTTGGAGTAAGTAAATATTAGTTTTTTCCACTAAATTTACGAAGTGGGCTTCCTCTGTCCAGTCATTATGATGATTCAGTAGTCAAAATTGTTCAAATCCAGCAGAAGGATAGTTAATATGCTCCAAATGACTATGGAGAAACAATATTTTTTCTCTGGCTTCTTGATAAGTTCTACATTCTGCTGAGCCTTTTTTTTTTTAGACTAAGAATTTAAGTAGTGAAAGAAAGCTCCTCCCGCTTGAATTAGCATCTCAGCTTGTTGCTGATGAACCATCTGTACATTGTAGCATTGCAAAGTTCGTTCATTCATTCATCGCAAGACGCAGTGCTGGAAGTTTGTTCTCGAGAGCAAGAAGTAGTGATTGCTTTAGGAAGCAATGTGGGTGATAGACTGCAGAATTTCAACGGAGCTTTGCGGTTGTTGAAAAAGGCAGGGATACACATTACAAGACATGCTTGTTTGTATGAGACAGCACCTGCTTATGTCACTAATCAACCTCAATTTCTCAACTCCGCTGTTAGAGCTGTCACAAAGCTTGGACCACATGAACTACTGAGTGCAGTTAAGAACATAGAGAAACAGCTGGGTCGTACTGCTGGTATACGCTACGGCCCGAGGCCGATTGACTTGGATATCTTGTTGTATGGAAGATATAAAGTACACTCAGATACCCTCACTGTCCCTCATGAAAGAATCTGGGAAAGGCCATTTGTGCTGGCTCCTTTAATTGATTTGCTGGGTTCAGATGTTGACACCGATGATGTTGCTTGCTGGCATTCTTTAGCTGCTGATCGTGGCGGTCTTTTTGAGTTATGGGAGAAAATGGGTGGTGAATCTCTTATTGGTAAAGAAGGAATGAGAAGGGTTTTGCCCATTGGAAACAACTTATGGGATTGGTCCTGCAAGACTTCCATCATGGGAGTTCTTAATTTGACACCTGACAGTTTTAGCGATGGTGGCAAGTTTCAACCTATTGAAGCTGCAGTTTCTCAGGTGCGTTCGATGGTTTCAGATGGTGCTGATATGATTGACATTGGTGCTCAGTCAACACGGCCCATGGCACCTATGATTTCTGTTGAAGAAGAATTGGATAGATTAGTTCCCGTTTTGGAAGCTGTTACAGGAATGCCAGAGATGAGTGGAAAGCTCATATCAGTGGATACGTTTTATTCAGAAGTCGCTTTGGAAGCTGTAAAGAGAGGGGCTCATATTGTAAATGACGTATCAGCGGGCCAGTTGGATCCTCAAATGCATAGGGTTGTTGCTCAGCTTAAGGTGCCTTATATTGCAATGCACATGAGAGGAGATCCATCTACAATGCAAAACAATGAGAATTTACAGTATGATGATGTTTGCAATGACATTGCCTCTGAGCTACACTCTAGGATTAGAGATGCAGAATTATCAGGCATCCCAGCTTGGAGGATAATTGTTGATCCTGGGATTGGATTCTCGAAGAACACAAAGCAAAACCTGGAAATTCTAGGAGGCGTACCAAAGATTCGAGCAGAAATTGCAAGGAGAAGTTTGGGATTGTCTCATGCTCCCATGTTGATTGGACCTTCGAGAAAGAAATTTCTCGGTGAAGTATGTTCGCAACCGATTGCAACGAAGCGAGATCCCGCTACAGTTGCTGCAGTTACCATAGGGGTTCTCGGTGGTGCAAACATAGCTAGAGTACATAACGTAAGAGATAATGTGGATGCAGTGAGGCTTTGTGATGCAATGCTGAAGGAGGAAACAAGCTGAAGCATGATTGTTTCTCGTTACACATCCTTTTCATTCTCATTTTTTTAATCAAATTATTATTTTTGGATAATTTGGTACACCGCTCGTGCTTTTACTGCTAGCGTCATAAGGAATAAAATGAGTTACTCGATGCCCCCTTGAATAGTATTGTCTCAAGTTATTGGTTGTCATTGATCCATCGGAAGATTGTCTGTCTCTCACTAGTGCCCCTAGGAGTATTTGTTTTCAAGGTTGCCTTTAAATATCATTTGAGTAATCAGCATAAGTATTGGC

mRNA sequence

GGAACTGGAAGACCGTAATTTCTGCTTCATTTTCTGGGCTTCTCACAAATGAACATTTTGAAGCATCCAATTATCAGCAGGCGAGGATTCAGATATGGTGGAGCATTGCAAAGTTCGTTCATTCATTCATCGCAAGACGCAGTGCTGGAAGTTTGTTCTCGAGAGCAAGAAGTAGTGATTGCTTTAGGAAGCAATGTGGGTGATAGACTGCAGAATTTCAACGGAGCTTTGCGGTTGTTGAAAAAGGCAGGGATACACATTACAAGACATGCTTGTTTGTATGAGACAGCACCTGCTTATGTCACTAATCAACCTCAATTTCTCAACTCCGCTGTTAGAGCTGTCACAAAGCTTGGACCACATGAACTACTGAGTGCAGTTAAGAACATAGAGAAACAGCTGGGTCGTACTGCTGGTATACGCTACGGCCCGAGGCCGATTGACTTGGATATCTTGTTGTATGGAAGATATAAAGTACACTCAGATACCCTCACTGTCCCTCATGAAAGAATCTGGGAAAGGCCATTTGTGCTGGCTCCTTTAATTGATTTGCTGGGTTCAGATGTTGACACCGATGATGTTGCTTGCTGGCATTCTTTAGCTGCTGATCGTGGCGGTCTTTTTGAGTTATGGGAGAAAATGGGTGGTGAATCTCTTATTGGTAAAGAAGGAATGAGAAGGGTTTTGCCCATTGGAAACAACTTATGGGATTGGTCCTGCAAGACTTCCATCATGGGAGTTCTTAATTTGACACCTGACAGTTTTAGCGATGGTGGCAAGTTTCAACCTATTGAAGCTGCAGTTTCTCAGGTGCGTTCGATGGTTTCAGATGGTGCTGATATGATTGACATTGGTGCTCAGTCAACACGGCCCATGGCACCTATGATTTCTGTTGAAGAAGAATTGGATAGATTAGTTCCCGTTTTGGAAGCTGTTACAGGAATGCCAGAGATGAGTGGAAAGCTCATATCAGTGGATACGTTTTATTCAGAAGTCGCTTTGGAAGCTGTAAAGAGAGGGGCTCATATTGTAAATGACGTATCAGCGGGCCAGTTGGATCCTCAAATGCATAGGGTTGTTGCTCAGCTTAAGGTGCCTTATATTGCAATGCACATGAGAGGAGATCCATCTACAATGCAAAACAATGAGAATTTACAGTATGATGATGTTTGCAATGACATTGCCTCTGAGCTACACTCTAGGATTAGAGATGCAGAATTATCAGGCATCCCAGCTTGGAGGATAATTGTTGATCCTGGGATTGGATTCTCGAAGAACACAAAGCAAAACCTGGAAATTCTAGGAGGCGTACCAAAGATTCGAGCAGAAATTGCAAGGAGAAGTTTGGGATTGTCTCATGCTCCCATGTTGATTGGACCTTCGAGAAAGAAATTTCTCGGTGAAGTATGTTCGCAACCGATTGCAACGAAGCGAGATCCCGCTACAGTTGCTGCAGTTACCATAGGGGTTCTCGGTGGTGCAAACATAGCTAGAGTACATAACGTAAGAGATAATGTGGATGCAGTGAGGCTTTGTGATGCAATGCTGAAGGAGGAAACAAGCTGAAGCATGATTGTTTCTCGTTACACATCCTTTTCATTCTCATTTTTTTAATCAAATTATTATTTTTGGATAATTTGGTACACCGCTCGTGCTTTTACTGCTAGCGTCATAAGGAATAAAATGAGTTACTCGATGCCCCCTTGAATAGTATTGTCTCAAGTTATTGGTTGTCATTGATCCATCGGAAGATTGTCTGTCTCTCACTAGTGCCCCTAGGAGTATTTGTTTTCAAGGTTGCCTTTAAATATCATTTGAGTAATCAGCATAAGTATTGGC

Coding sequence (CDS)

ATGAACATTTTGAAGCATCCAATTATCAGCAGGCGAGGATTCAGATATGGTGGAGCATTGCAAAGTTCGTTCATTCATTCATCGCAAGACGCAGTGCTGGAAGTTTGTTCTCGAGAGCAAGAAGTAGTGATTGCTTTAGGAAGCAATGTGGGTGATAGACTGCAGAATTTCAACGGAGCTTTGCGGTTGTTGAAAAAGGCAGGGATACACATTACAAGACATGCTTGTTTGTATGAGACAGCACCTGCTTATGTCACTAATCAACCTCAATTTCTCAACTCCGCTGTTAGAGCTGTCACAAAGCTTGGACCACATGAACTACTGAGTGCAGTTAAGAACATAGAGAAACAGCTGGGTCGTACTGCTGGTATACGCTACGGCCCGAGGCCGATTGACTTGGATATCTTGTTGTATGGAAGATATAAAGTACACTCAGATACCCTCACTGTCCCTCATGAAAGAATCTGGGAAAGGCCATTTGTGCTGGCTCCTTTAATTGATTTGCTGGGTTCAGATGTTGACACCGATGATGTTGCTTGCTGGCATTCTTTAGCTGCTGATCGTGGCGGTCTTTTTGAGTTATGGGAGAAAATGGGTGGTGAATCTCTTATTGGTAAAGAAGGAATGAGAAGGGTTTTGCCCATTGGAAACAACTTATGGGATTGGTCCTGCAAGACTTCCATCATGGGAGTTCTTAATTTGACACCTGACAGTTTTAGCGATGGTGGCAAGTTTCAACCTATTGAAGCTGCAGTTTCTCAGGTGCGTTCGATGGTTTCAGATGGTGCTGATATGATTGACATTGGTGCTCAGTCAACACGGCCCATGGCACCTATGATTTCTGTTGAAGAAGAATTGGATAGATTAGTTCCCGTTTTGGAAGCTGTTACAGGAATGCCAGAGATGAGTGGAAAGCTCATATCAGTGGATACGTTTTATTCAGAAGTCGCTTTGGAAGCTGTAAAGAGAGGGGCTCATATTGTAAATGACGTATCAGCGGGCCAGTTGGATCCTCAAATGCATAGGGTTGTTGCTCAGCTTAAGGTGCCTTATATTGCAATGCACATGAGAGGAGATCCATCTACAATGCAAAACAATGAGAATTTACAGTATGATGATGTTTGCAATGACATTGCCTCTGAGCTACACTCTAGGATTAGAGATGCAGAATTATCAGGCATCCCAGCTTGGAGGATAATTGTTGATCCTGGGATTGGATTCTCGAAGAACACAAAGCAAAACCTGGAAATTCTAGGAGGCGTACCAAAGATTCGAGCAGAAATTGCAAGGAGAAGTTTGGGATTGTCTCATGCTCCCATGTTGATTGGACCTTCGAGAAAGAAATTTCTCGGTGAAGTATGTTCGCAACCGATTGCAACGAAGCGAGATCCCGCTACAGTTGCTGCAGTTACCATAGGGGTTCTCGGTGGTGCAAACATAGCTAGAGTACATAACGTAAGAGATAATGTGGATGCAGTGAGGCTTTGTGATGCAATGCTGAAGGAGGAAACAAGCTGA

Protein sequence

MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAMLKEETS

Homology

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BLAST of CmaCh04G000250 vs. ExPASy Swiss-Prot
Match: O04862 (Folate synthesis bifunctional protein, mitochondrial OS=Pisum sativum OX=3888 GN=MitHPPK/DHPS PE=1 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.1e-208
Identity = 345/490 (70.41%), Postives = 424/490 (86.53%), Query Frame = 0


Query: 16  YGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHA 75
           Y   L  S  H++ ++ +E+ ++++EVVIALGSNVGDRL NF  AL+L++K+GIHITRHA
Sbjct: 20  YLKVLGFSSFHTAPNSSIEIQTQDEEVVIALGSNVGDRLHNFKEALKLMRKSGIHITRHA 79

Query: 76  CLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDI 135
            LYETAPAYVT+QP+FLNSAVRA TKLGPHELL+A+K IEK +GRT GIRYGPRPIDLDI
Sbjct: 80  SLYETAPAYVTDQPRFLNSAVRADTKLGPHELLAALKRIEKDMGRTDGIRYGPRPIDLDI 139

Query: 136 LLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELW 195
           L YG++KV SD LTVPHERIWERPFV+APL+DLLG+ +D+D VA WHS +   GGL  LW
Sbjct: 140 LFYGKFKVRSDILTVPHERIWERPFVMAPLMDLLGTAIDSDTVASWHSFSGHSGGLNALW 199

Query: 196 EKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQV 255
           EK+GGESLIG+EGM RV+P+ N L DWS +T +MG+LNLTPDSFSDGG FQ +++AVSQ 
Sbjct: 200 EKLGGESLIGEEGMYRVMPVANGLLDWSRRTLVMGILNLTPDSFSDGGNFQSVKSAVSQA 259

Query: 256 RSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSE 315
           R M+S+GAD+IDIGAQSTRPMA  IS EEEL RL+PVLEAV  +PE+ GKLISVDTFYSE
Sbjct: 260 RLMISEGADIIDIGAQSTRPMASRISAEEELGRLIPVLEAVMSIPEVEGKLISVDTFYSE 319

Query: 316 VALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVC 375
           VALEAV++GAHI+NDVSAG+LD  M +V+A+L VPY+AMHMRGDPSTMQ++ENL+YD+VC
Sbjct: 320 VALEAVRKGAHIINDVSAGKLDASMFKVMAELDVPYVAMHMRGDPSTMQDSENLKYDNVC 379

Query: 376 NDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGL 435
            DI+SEL+SR+R+AE+SGIPAWRII+DPGIGFSK T+ NL  L G+P IR EI++RSL +
Sbjct: 380 KDISSELYSRVREAEISGIPAWRIIMDPGIGFSKKTEDNLAALTGIPDIREEISKRSLAI 439

Query: 436 SHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRL 495
           SHAP+LIGPSRK+FLGE+CS+P A  RDPAT+A+VT GVL GANI RVHNV+DN+DAV+L
Sbjct: 440 SHAPILIGPSRKRFLGEICSRPSAVDRDPATIASVTAGVLCGANIVRVHNVKDNLDAVKL 499

Query: 496 CDAMLKEETS 506
           CDA+LK+++S
Sbjct: 500 CDAILKQKSS 509

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BLAST of CmaCh04G000250 vs. ExPASy Swiss-Prot
Match: Q9SZV3 (Folate synthesis bifunctional protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MitHPPK/DHPS PE=2 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 1.1e-201
Identity = 333/484 (68.80%), Postives = 407/484 (84.09%), Query Frame = 0


Query: 22  SSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLYETA 81
           S+F  S+    +EV S E EVVIALGSN+G+R+ NF  ALRL+K+ GI +TRH+CLYETA
Sbjct: 69  SAFSSSATSTTIEVQSTEHEVVIALGSNIGNRMNNFREALRLMKRGGICVTRHSCLYETA 128

Query: 82  PAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRY 141
           P +VT+QP+FLN+AVR VTKLGPHELLS +K IE+ +GR  GIRYGPRP+DLDIL YG+ 
Sbjct: 129 PVHVTDQPRFLNAAVRGVTKLGPHELLSVLKTIERDMGRKDGIRYGPRPLDLDILFYGKM 188

Query: 142 KVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGE 201
           ++ SD L +PHER+WER FVLAPL+DLLGS VD D VA WHSLA   GG+F+ WE++GGE
Sbjct: 189 RISSDKLIIPHERLWERSFVLAPLVDLLGSAVDNDTVAHWHSLAIHPGGIFQAWERLGGE 248

Query: 202 SLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSD 261
           SLIG++G++RVLPIG+ LWD+S KT +MG+LNLTPDSFSDGGKFQ I++AVS+VRSM+S+
Sbjct: 249 SLIGQDGIQRVLPIGDKLWDFSNKTHVMGILNLTPDSFSDGGKFQSIDSAVSRVRSMISE 308

Query: 262 GADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAV 321
           GAD+IDIGAQSTRPMA  IS +EELDRL+PVLEAV GMPEM  KLISVDTF SEVA EA+
Sbjct: 309 GADIIDIGAQSTRPMASRISSQEELDRLLPVLEAVRGMPEMEEKLISVDTFNSEVASEAI 368

Query: 322 KRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASE 381
             GA I+NDVSAG LDP MH+VVA+  VPY+AMHMRGDP TMQN ENLQYDDVC D+ASE
Sbjct: 369 SNGADILNDVSAGTLDPNMHKVVAESGVPYMAMHMRGDPCTMQNKENLQYDDVCKDVASE 428

Query: 382 LHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPML 441
           L+ R+RDAELSGIPAWR+++DPGIGFSK+   NL+I+  +PKIR E+A+RS+ +SHAP+L
Sbjct: 429 LYLRVRDAELSGIPAWRVMIDPGIGFSKSVDHNLDIIMDLPKIREEMAKRSIAVSHAPIL 488

Query: 442 IGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAMLK 501
           +GPSRK+FLG++C +P AT RD ATVA+VT G+LGGANI RVHNVR N DA ++CDAML+
Sbjct: 489 VGPSRKRFLGDICGRPEATDRDAATVASVTAGILGGANIIRVHNVRHNADAAKVCDAMLR 548

Query: 502 EETS 506
              S
Sbjct: 549 RRRS 552

[Close]

BLAST of CmaCh04G000250 vs. ExPASy Swiss-Prot
Match: Q1ENB6 (Folate synthesis bifunctional protein OS=Arabidopsis thaliana OX=3702 GN=CytHPPK/DHPS PE=1 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 8.3e-189
Identity = 313/465 (67.31%), Postives = 394/465 (84.73%), Query Frame = 0


Query: 40  QEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAV 99
           +EVVIALGSNVG+R+ NF  ALRL+K  GI +TRH+CLYET P +VT+QP+FLN+A+R V
Sbjct: 12  EEVVIALGSNVGNRMNNFKEALRLMKDYGISVTRHSCLYETEPVHVTDQPRFLNAAIRGV 71

Query: 100 TKLGPHELLSAVKNIEKQLGRTA-GIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWER 159
           TKL PHELL+ +K IEK++GR   G+RYGPRP+DLDIL YG++K+ SD L +PHERIWER
Sbjct: 72  TKLKPHELLNVLKKIEKEMGREENGLRYGPRPLDLDILFYGKHKIISDKLIIPHERIWER 131

Query: 160 PFVLAPLIDLLGS-DVDTDD-VACWHSLAADRGGLFELWEKMGGESLIGKEG-MRRVLPI 219
           PFVLAPL+DLLG+ D+D D  VA WHSL+   GG+F+ WE++GGESL+GK+G ++RV+PI
Sbjct: 132 PFVLAPLVDLLGTEDIDNDKIVAYWHSLSMHSGGIFQAWERLGGESLLGKDGIIQRVIPI 191

Query: 220 GNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRP 279
           G++LWD+S KT +MG+LNLTPDSFSDGGKFQ ++ AVS+VRSM+S+G D+IDIGAQSTRP
Sbjct: 192 GDHLWDFSKKTYVMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRP 251

Query: 280 MAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQ 339
           MA  IS +EE+DRL+PVL+ V GM EM GKLISVDTF SEVALEA++ GA I+NDVS G 
Sbjct: 252 MASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGS 311

Query: 340 LDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIP 399
           LD  MH+VVA   VPY+ MHMRGDP TMQN ENL+Y+++C D+A+EL+ R+R+AELSGIP
Sbjct: 312 LDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIP 371

Query: 400 AWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCS 459
           AWRI++DPGIGFSK    NL+I+  +PKIR E+A++S+GLSHAP+LIGPSRK+FLG++C 
Sbjct: 372 AWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICG 431

Query: 460 QPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAML 501
           +P A++RD ATVA VT G+L GANI RVHNVRDNVDA RLCDAM+
Sbjct: 432 RPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAARLCDAMM 476

[Close]

BLAST of CmaCh04G000250 vs. ExPASy Swiss-Prot
Match: P29251 (Folic acid synthesis protein fol1 OS=Pneumocystis carinii OX=4754 GN=fol1 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.0e-82
Identity = 186/471 (39.49%), Postives = 270/471 (57.32%), Query Frame = 0


Query: 40  QEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAV 99
           + V I+LGSN+G+R++    A+  +   GI + + + LYE+ P Y  +QP F N+  +  
Sbjct: 294 EAVYISLGSNLGNRIKFILDAIEKMSIKGIKVLKTSMLYESKPMYFKDQPAFYNAVCKVQ 353

Query: 100 TKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERP 159
           T L P +LL  ++ IEK+LGR   I  GPR IDLDI+ YGR  ++S++L +PH R+ ER 
Sbjct: 354 TSLHPEQLLFELQLIEKELGRVKVIDKGPRCIDLDIVFYGRKIINSESLIIPHPRVLERS 413

Query: 160 FVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLP----- 219
           FVL PL+D+ G  V        H +      +   +EK      I    ++ VLP     
Sbjct: 414 FVLKPLLDISGDLV--------HPVTG--LSIASYFEK------IVDHDIKPVLPFLYKN 473

Query: 220 --IGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQS 279
             I  +   +   T IM +LNLTPDSF DGG     ++ +  V   ++ GA +IDIG QS
Sbjct: 474 KSIDFSFRSYKAPTYIMAILNLTPDSFFDGG-IHSYDSVLIDVEKFINAGATIIDIGGQS 533

Query: 280 TRPMAPMISVEEELDRLVPVLEAV-TGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDV 339
           TRP + +I +EEE+ R++P ++ +    P++   LIS+DTF SEVA +AVK GA +VND+
Sbjct: 534 TRPGSYIIPLEEEIFRVIPAIKYLQKTYPDI---LISIDTFRSEVAEQAVKAGASLVNDI 593

Query: 340 SAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASELHSRIRDAEL 399
           S G+ DP+M   VA+LKVP   MHMRG+   M N  +    D+   I  EL   +  AE 
Sbjct: 594 SGGRYDPKMFNTVARLKVPICIMHMRGNFLNMDNLTDYG-TDIIEQITIELEKLLNSAEK 653

Query: 400 SGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLG 459
           SGIP W II+DPG+GFSK   QN+E+L    +++++     L     P L+GPSRK+F G
Sbjct: 654 SGIPRWNIILDPGLGFSKTLHQNIELLRRFNELKSKNCFNGL-----PWLLGPSRKRFTG 713

Query: 460 EVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAMLKE 503
            +    +   R   TVAAV   + GG +I RVH+V +     ++ DA+ KE
Sbjct: 714 FITGDNMPKDRIWGTVAAVVASISGGCDIIRVHDVYEMYKISKMSDAIWKE 738

[Close]

BLAST of CmaCh04G000250 vs. ExPASy Swiss-Prot
Match: Q4LB35 (Folic acid synthesis protein fol1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fol1 PE=1 SV=2)

HSP 1 Score: 271.2 bits (692), Expect = 2.4e-71
Identity = 174/459 (37.91%), Postives = 255/459 (55.56%), Query Frame = 0


Query: 44  IALGSNVGDRLQNFNGALRLLKK-AGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKL 103
           ++ GSN+GD+ +    AL +L K  GI +   + LYET P Y  +QP FLN   +  T++
Sbjct: 300 LSFGSNIGDKFEQIQTALSMLHKIEGIRVLDVSPLYETEPMYYKDQPSFLNGVCKIETRM 359

Query: 104 GPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVL 163
            P  LL A ++IE+++GR   I  GPR IDLDI+LY      S+ LT+PH  + ER FVL
Sbjct: 360 SPINLLRACQSIEQEMGRIKTILKGPRCIDLDIVLYEDCVYESEVLTIPHLGLQEREFVL 419

Query: 164 APLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDW 223
            PL+ L      + D+   ++       L E  +K      +  +G+R      N     
Sbjct: 420 RPLLAL------SPDLVHPYT----HQPLQEALDK------LPSQGIRLYSSFDNKKIIN 479

Query: 224 SCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISV 283
              T  MG+LN+TPDSFSDGGK       + + +SMV DGA ++DIG QST+P A  +SV
Sbjct: 480 GALT--MGILNVTPDSFSDGGKVSQ-NNILEKAKSMVGDGASILDIGGQSTKPGADPVSV 539

Query: 284 EEELDRLVPVLEAVTGMPEMSGKL--ISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQM 343
           EEEL R++P++  +      SG    IS+DT+YS+VA  A++ GA+I+NDV+ G  D +M
Sbjct: 540 EEELRRVIPMISLL----RSSGITVPISIDTYYSKVAKLAIEAGANIINDVTGGMGDEKM 599

Query: 344 HRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRII 403
             + A L+VP   MHMRG P TM+   ++   D+  ++A EL SR+  A  SG+  + II
Sbjct: 600 LPLAASLQVPICIMHMRGTPETMK-ALSIYEKDIVEEVAVELSSRVEAAVQSGVHRYNII 659

Query: 404 VDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIAT 463
           +DPG GF+K  KQ+  +LG + ++  +   + +       L GPSRK F G         
Sbjct: 660 LDPGFGFAKTPKQSAGLLGRLHELMKKPQFKDM-----HWLSGPSRKGFTGYFTGDASPK 719

Query: 464 KRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAM 500
            R   T A VT  VL G +I RVH+ ++    V + +A+
Sbjct: 720 DRIWGTSACVTASVLQGVSIVRVHDTKEMSKVVGMANAI 729

[Close]

BLAST of CmaCh04G000250 vs. ExPASy TrEMBL
Match: A0A6J1JQH6 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111487416 PE=3 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 1.9e-292
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60
           MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA
Sbjct: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60

Query: 61  LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120
           LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR
Sbjct: 61  LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120

Query: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180
           TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC
Sbjct: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180

Query: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240
           WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS
Sbjct: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240

Query: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300
           DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP
Sbjct: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300

Query: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360
           EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP
Sbjct: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360

Query: 361 STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420
           STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG
Sbjct: 361 STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420

Query: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480
           VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI
Sbjct: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480

Query: 481 ARVHNVRDNVDAVRLCDAMLKEETS 506
           ARVHNVRDNVDAVRLCDAMLKEETS
Sbjct: 481 ARVHNVRDNVDAVRLCDAMLKEETS 505

[Close]

BLAST of CmaCh04G000250 vs. ExPASy TrEMBL
Match: A0A6J1FP77 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111447567 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.6e-286
Identity = 495/505 (98.02%), Postives = 502/505 (99.41%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60
           MNILKHPIISRRGFRYGGALQSSF+HSSQ AVLEVCS+EQEVVIALGSNVGDRLQNFN A
Sbjct: 1   MNILKHPIISRRGFRYGGALQSSFVHSSQGAVLEVCSQEQEVVIALGSNVGDRLQNFNEA 60

Query: 61  LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120
           L+L+KKAGIHITRHA LYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR
Sbjct: 61  LQLMKKAGIHITRHAGLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120

Query: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180
           TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC
Sbjct: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180

Query: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240
           W+SLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS
Sbjct: 181 WNSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240

Query: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300
           DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP
Sbjct: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300

Query: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360
           EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP
Sbjct: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360

Query: 361 STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420
           STMQNNENLQYDDVCN+IASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG
Sbjct: 361 STMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420

Query: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480
           VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI
Sbjct: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480

Query: 481 ARVHNVRDNVDAVRLCDAMLKEETS 506
           ARVHNVRDNVDAVRLCDAMLKE+TS
Sbjct: 481 ARVHNVRDNVDAVRLCDAMLKEKTS 505

[Close]

BLAST of CmaCh04G000250 vs. ExPASy TrEMBL
Match: A0A1S4E253 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103496695 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.4e-263
Identity = 452/506 (89.33%), Postives = 476/506 (94.07%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIH-SSQDAVLEVCSREQEVVIALGSNVGDRLQNFNG 60
           MNILKH II R+GFRYGGALQ SF H SSQD V+E+CSREQEVVIALGSNVGDRLQNFN 
Sbjct: 1   MNILKHRIIGRQGFRYGGALQISFFHSSSQDKVVEICSREQEVVIALGSNVGDRLQNFNE 60

Query: 61  ALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120
           ALRL+KKAGIHITRHACLYETAPAYVT+QPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG
Sbjct: 61  ALRLMKKAGIHITRHACLYETAPAYVTDQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120

Query: 121 RTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVA 180
           RTAGIRYGPRPIDLDILLYGRYK+HSD LT+PHERIWERPFVLAPLIDLLGSDVDTDDVA
Sbjct: 121 RTAGIRYGPRPIDLDILLYGRYKIHSDILTIPHERIWERPFVLAPLIDLLGSDVDTDDVA 180

Query: 181 CWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSF 240
            WHSLAAD GGLFE WEK+GGE L+GKEGMRRVL +GN+LWDWSCKTS+MGVLNLTPDSF
Sbjct: 181 SWHSLAADHGGLFESWEKVGGEYLVGKEGMRRVLSVGNSLWDWSCKTSVMGVLNLTPDSF 240

Query: 241 SDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGM 300
           SDGGKFQ IEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRL+PVLEAVT M
Sbjct: 241 SDGGKFQSIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLIPVLEAVTRM 300

Query: 301 PEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGD 360
           PEM GKLISVDTFYS+VALEAVKRGAHIVNDVSAG LDP+MH+VVA L VPYIAMHMRGD
Sbjct: 301 PEMGGKLISVDTFYSKVALEAVKRGAHIVNDVSAGNLDPEMHKVVADLNVPYIAMHMRGD 360

Query: 361 PSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILG 420
           PSTMQNNENLQYDDVCN IA ELHS+IRDAE SGIPAWRII+DPG+GFSK TKQNLEIL 
Sbjct: 361 PSTMQNNENLQYDDVCNQIALELHSKIRDAESSGIPAWRIIIDPGVGFSKTTKQNLEILT 420

Query: 421 GVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGAN 480
           GVPKIR  IARRSLGLSHAPMLIGPSRKKFLGEVCS+ +AT+RDPATVAAVT+GVLGGAN
Sbjct: 421 GVPKIRTAIARRSLGLSHAPMLIGPSRKKFLGEVCSRSVATERDPATVAAVTVGVLGGAN 480

Query: 481 IARVHNVRDNVDAVRLCDAMLKEETS 506
           I RVHNVRDNVDAVRLCDAM KE+ S
Sbjct: 481 IVRVHNVRDNVDAVRLCDAMQKEKKS 506

[Close]

BLAST of CmaCh04G000250 vs. ExPASy TrEMBL
Match: A0A0A0KP07 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis sativus OX=3659 GN=Csa_5G190480 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.0e-262
Identity = 450/506 (88.93%), Postives = 479/506 (94.66%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIH-SSQDAVLEVCSREQEVVIALGSNVGDRLQNFNG 60
           MNILK  IIS++GFRYGGALQ SF+H SSQD V+E+CS+EQEVVIALGSNVGDRLQNFN 
Sbjct: 1   MNILKRCIISKQGFRYGGALQISFLHSSSQDKVVEICSQEQEVVIALGSNVGDRLQNFNE 60

Query: 61  ALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120
           ALRL+KKAGIHITRHACLYETAPAYVT+QPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG
Sbjct: 61  ALRLMKKAGIHITRHACLYETAPAYVTDQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120

Query: 121 RTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVA 180
           RTAGIRYGPRPIDLDILLYGRYKVHSDTLT+PHERIWERPFVLAPLIDLLGSDVDTDDVA
Sbjct: 121 RTAGIRYGPRPIDLDILLYGRYKVHSDTLTIPHERIWERPFVLAPLIDLLGSDVDTDDVA 180

Query: 181 CWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSF 240
            WHSLAAD GGLFE WEK+GGE L+GKEGMRRVL IGN+LWDWSCKTS+MGVLNLTPDSF
Sbjct: 181 SWHSLAADHGGLFESWEKVGGEYLVGKEGMRRVLSIGNSLWDWSCKTSVMGVLNLTPDSF 240

Query: 241 SDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGM 300
           SDGGKFQ IEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRL+PVLEAVT M
Sbjct: 241 SDGGKFQSIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLIPVLEAVTRM 300

Query: 301 PEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGD 360
           PEMSGKLISVDTFYS+VALEAVKRGAHIVNDVSAG LDP+MH+VVA L VPYIAMHMRGD
Sbjct: 301 PEMSGKLISVDTFYSKVALEAVKRGAHIVNDVSAGNLDPEMHKVVADLNVPYIAMHMRGD 360

Query: 361 PSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILG 420
           PSTMQN ENLQYDDVCN IA ELHS+IRDAE SGIPAWRII+DPG+GFSK TKQNLEIL 
Sbjct: 361 PSTMQNKENLQYDDVCNQIALELHSKIRDAESSGIPAWRIIIDPGVGFSKTTKQNLEILT 420

Query: 421 GVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGAN 480
           G+PKIRA IA+RSLGLSHAPMLIGPSRKKFLGEVCS+ +AT+RDPAT+AAVT+GVLGGAN
Sbjct: 421 GIPKIRAAIAKRSLGLSHAPMLIGPSRKKFLGEVCSRSVATERDPATIAAVTVGVLGGAN 480

Query: 481 IARVHNVRDNVDAVRLCDAMLKEETS 506
           I RVHNVR+NVDAVRLCDAM KE+ S
Sbjct: 481 IVRVHNVRNNVDAVRLCDAMQKEKKS 506

[Close]

BLAST of CmaCh04G000250 vs. ExPASy TrEMBL
Match: A0A6J1DBE6 (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Momordica charantia OX=3673 GN=LOC111018761 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.5e-257
Identity = 446/507 (87.97%), Postives = 469/507 (92.50%), Query Frame = 0


Query: 1   MNILKHPIISRRGF----RYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQN 60
           MNILKH IISRR F    RYGGA Q SFIHS QD +LEVCS+EQEVVIALGSNVGDRL N
Sbjct: 1   MNILKHGIISRRRFSRVTRYGGASQISFIHSLQDPLLEVCSQEQEVVIALGSNVGDRLYN 60

Query: 61  FNGALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEK 120
           FN AL+L+K+ GIHITRHACLYETAPAYVT+QPQFLNSAVRA TKLGPHELLS VK IEK
Sbjct: 61  FNEALKLMKREGIHITRHACLYETAPAYVTDQPQFLNSAVRAFTKLGPHELLSTVKRIEK 120

Query: 121 QLGRTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTD 180
           +LGRTAGIRYGPRPIDLDILLYGRY+VHSD LTVPHERIWERPFVLAPLIDLLGSDVDTD
Sbjct: 121 ELGRTAGIRYGPRPIDLDILLYGRYRVHSDVLTVPHERIWERPFVLAPLIDLLGSDVDTD 180

Query: 181 DVACWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTP 240
            V+ WHSLA D GGLFE WEK+GGESLIGKEGMRRVLPIGN+LWDWSCKTSIMG+LNLTP
Sbjct: 181 VVSGWHSLAPDHGGLFESWEKLGGESLIGKEGMRRVLPIGNSLWDWSCKTSIMGILNLTP 240

Query: 241 DSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAV 300
           DSFSDGGKFQ +EAAVSQVRSMVSDGADMIDIGAQSTRPMAP ISVEEELDRLVPVLEAV
Sbjct: 241 DSFSDGGKFQSVEAAVSQVRSMVSDGADMIDIGAQSTRPMAPRISVEEELDRLVPVLEAV 300

Query: 301 TGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHM 360
           TGMPEMSGKLISVDTFYSEVALEAVK+GAHIVNDVSAGQLDPQMH VV+ L VPYIAMHM
Sbjct: 301 TGMPEMSGKLISVDTFYSEVALEAVKKGAHIVNDVSAGQLDPQMHSVVSDLDVPYIAMHM 360

Query: 361 RGDPSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLE 420
           RGDP TMQN +NLQYDDVCN IASELH RIRDAE SGIPAWRIIVDPGIGFSKNTKQNLE
Sbjct: 361 RGDPYTMQNTQNLQYDDVCNQIASELHLRIRDAESSGIPAWRIIVDPGIGFSKNTKQNLE 420

Query: 421 ILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLG 480
           IL G+PKIRAEIARRSLGLSHAPML GPSRKKFLGEVCS+ +AT+RDPATVAA+TIGVLG
Sbjct: 421 ILTGLPKIRAEIARRSLGLSHAPMLFGPSRKKFLGEVCSRSVATERDPATVAAITIGVLG 480

Query: 481 GANIARVHNVRDNVDAVRLCDAMLKEE 504
           GANI RVHNVRDN DAV LCDAM+KE+
Sbjct: 481 GANIVRVHNVRDNADAVSLCDAMVKEK 507

[Close]

BLAST of CmaCh04G000250 vs. NCBI nr
Match: XP_022990585.1 (folate synthesis bifunctional protein, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 1014.2 bits (2621), Expect = 3.8e-292
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60
           MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA
Sbjct: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60

Query: 61  LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120
           LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR
Sbjct: 61  LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120

Query: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180
           TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC
Sbjct: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180

Query: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240
           WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS
Sbjct: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240

Query: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300
           DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP
Sbjct: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300

Query: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360
           EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP
Sbjct: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360

Query: 361 STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420
           STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG
Sbjct: 361 STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420

Query: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480
           VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI
Sbjct: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480

Query: 481 ARVHNVRDNVDAVRLCDAMLKEETS 506
           ARVHNVRDNVDAVRLCDAMLKEETS
Sbjct: 481 ARVHNVRDNVDAVRLCDAMLKEETS 505

[Close]

BLAST of CmaCh04G000250 vs. NCBI nr
Match: XP_023536333.1 (folate synthesis bifunctional protein, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.1e-290
Identity = 500/505 (99.01%), Postives = 505/505 (100.00%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60
           MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA
Sbjct: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60

Query: 61  LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120
           L+L+KKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR
Sbjct: 61  LQLMKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120

Query: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180
           TAGIRYGPRPIDLDILLYGRYK+HSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC
Sbjct: 121 TAGIRYGPRPIDLDILLYGRYKIHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180

Query: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240
           WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS
Sbjct: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240

Query: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300
           DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP
Sbjct: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300

Query: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360
           EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP
Sbjct: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360

Query: 361 STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420
           STMQNNENLQYDDVCN+IASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG
Sbjct: 361 STMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420

Query: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480
           VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI
Sbjct: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480

Query: 481 ARVHNVRDNVDAVRLCDAMLKEETS 506
           ARVHNVRDNVDAVRLCDAMLKE+TS
Sbjct: 481 ARVHNVRDNVDAVRLCDAMLKEKTS 505

[Close]

BLAST of CmaCh04G000250 vs. NCBI nr
Match: XP_022942566.1 (folate synthesis bifunctional protein, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 993.8 bits (2568), Expect = 5.4e-286
Identity = 495/505 (98.02%), Postives = 502/505 (99.41%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGA 60
           MNILKHPIISRRGFRYGGALQSSF+HSSQ AVLEVCS+EQEVVIALGSNVGDRLQNFN A
Sbjct: 1   MNILKHPIISRRGFRYGGALQSSFVHSSQGAVLEVCSQEQEVVIALGSNVGDRLQNFNEA 60

Query: 61  LRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120
           L+L+KKAGIHITRHA LYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR
Sbjct: 61  LQLMKKAGIHITRHAGLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGR 120

Query: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180
           TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC
Sbjct: 121 TAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVAC 180

Query: 181 WHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240
           W+SLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS
Sbjct: 181 WNSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFS 240

Query: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300
           DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP
Sbjct: 241 DGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMP 300

Query: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360
           EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP
Sbjct: 301 EMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDP 360

Query: 361 STMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420
           STMQNNENLQYDDVCN+IASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG
Sbjct: 361 STMQNNENLQYDDVCNEIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGG 420

Query: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480
           VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI
Sbjct: 421 VPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANI 480

Query: 481 ARVHNVRDNVDAVRLCDAMLKEETS 506
           ARVHNVRDNVDAVRLCDAMLKE+TS
Sbjct: 481 ARVHNVRDNVDAVRLCDAMLKEKTS 505

[Close]

BLAST of CmaCh04G000250 vs. NCBI nr
Match: XP_008456884.1 (PREDICTED: folate synthesis bifunctional protein, mitochondrial-like [Cucumis melo] >XP_016902055.1 PREDICTED: folate synthesis bifunctional protein, mitochondrial-like [Cucumis melo])

HSP 1 Score: 918.3 bits (2372), Expect = 2.9e-263
Identity = 452/506 (89.33%), Postives = 476/506 (94.07%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIH-SSQDAVLEVCSREQEVVIALGSNVGDRLQNFNG 60
           MNILKH II R+GFRYGGALQ SF H SSQD V+E+CSREQEVVIALGSNVGDRLQNFN 
Sbjct: 1   MNILKHRIIGRQGFRYGGALQISFFHSSSQDKVVEICSREQEVVIALGSNVGDRLQNFNE 60

Query: 61  ALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120
           ALRL+KKAGIHITRHACLYETAPAYVT+QPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG
Sbjct: 61  ALRLMKKAGIHITRHACLYETAPAYVTDQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120

Query: 121 RTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVA 180
           RTAGIRYGPRPIDLDILLYGRYK+HSD LT+PHERIWERPFVLAPLIDLLGSDVDTDDVA
Sbjct: 121 RTAGIRYGPRPIDLDILLYGRYKIHSDILTIPHERIWERPFVLAPLIDLLGSDVDTDDVA 180

Query: 181 CWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSF 240
            WHSLAAD GGLFE WEK+GGE L+GKEGMRRVL +GN+LWDWSCKTS+MGVLNLTPDSF
Sbjct: 181 SWHSLAADHGGLFESWEKVGGEYLVGKEGMRRVLSVGNSLWDWSCKTSVMGVLNLTPDSF 240

Query: 241 SDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGM 300
           SDGGKFQ IEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRL+PVLEAVT M
Sbjct: 241 SDGGKFQSIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLIPVLEAVTRM 300

Query: 301 PEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGD 360
           PEM GKLISVDTFYS+VALEAVKRGAHIVNDVSAG LDP+MH+VVA L VPYIAMHMRGD
Sbjct: 301 PEMGGKLISVDTFYSKVALEAVKRGAHIVNDVSAGNLDPEMHKVVADLNVPYIAMHMRGD 360

Query: 361 PSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILG 420
           PSTMQNNENLQYDDVCN IA ELHS+IRDAE SGIPAWRII+DPG+GFSK TKQNLEIL 
Sbjct: 361 PSTMQNNENLQYDDVCNQIALELHSKIRDAESSGIPAWRIIIDPGVGFSKTTKQNLEILT 420

Query: 421 GVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGAN 480
           GVPKIR  IARRSLGLSHAPMLIGPSRKKFLGEVCS+ +AT+RDPATVAAVT+GVLGGAN
Sbjct: 421 GVPKIRTAIARRSLGLSHAPMLIGPSRKKFLGEVCSRSVATERDPATVAAVTVGVLGGAN 480

Query: 481 IARVHNVRDNVDAVRLCDAMLKEETS 506
           I RVHNVRDNVDAVRLCDAM KE+ S
Sbjct: 481 IVRVHNVRDNVDAVRLCDAMQKEKKS 506

[Close]

BLAST of CmaCh04G000250 vs. NCBI nr
Match: XP_038892551.1 (folate synthesis bifunctional protein, mitochondrial-like isoform X1 [Benincasa hispida])

HSP 1 Score: 914.8 bits (2363), Expect = 3.2e-262
Identity = 449/503 (89.26%), Postives = 475/503 (94.43%), Query Frame = 0


Query: 1   MNILKHPIISRRGFRYGGALQSSFIH-SSQDAVLEVCSREQEVVIALGSNVGDRLQNFNG 60
           MNILKH IISRRGF YGGALQ SFIH SSQD V+EVCS+EQE+ IALGSNVGDRLQNFN 
Sbjct: 1   MNILKHRIISRRGFIYGGALQISFIHSSSQDKVVEVCSKEQEIAIALGSNVGDRLQNFNE 60

Query: 61  ALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120
           ALRL+KK+GIHITRHACLYETAPAYVT+QPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG
Sbjct: 61  ALRLMKKSGIHITRHACLYETAPAYVTDQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLG 120

Query: 121 RTAGIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVA 180
           RTAGIRYGPRPIDLDILLYG+YKVHSD LTVPHERIWERPFVLAPLIDLLGSDVDTD VA
Sbjct: 121 RTAGIRYGPRPIDLDILLYGKYKVHSDILTVPHERIWERPFVLAPLIDLLGSDVDTDVVA 180

Query: 181 CWHSLAADRGGLFELWEKMGGESLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSF 240
            WH+LAAD GGLFE WEK GGE L+GKEGMRRVLPIGN+LWDWSCKTS+MGVLNLTPDSF
Sbjct: 181 SWHALAADHGGLFETWEKAGGECLVGKEGMRRVLPIGNSLWDWSCKTSVMGVLNLTPDSF 240

Query: 241 SDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGM 300
           SDGGKFQ I+AAVSQVR MVSDGADMIDIGAQSTRPMAPMISVEEELDRL+PVLEA+TGM
Sbjct: 241 SDGGKFQSIDAAVSQVRLMVSDGADMIDIGAQSTRPMAPMISVEEELDRLIPVLEAITGM 300

Query: 301 PEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGD 360
           PEMSGKLISVDTFYS+VA EAVK GAHIVNDVSAG+LDP+MH+VVA L VPYIAMHMRGD
Sbjct: 301 PEMSGKLISVDTFYSKVASEAVKSGAHIVNDVSAGELDPEMHKVVADLNVPYIAMHMRGD 360

Query: 361 PSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILG 420
           P TMQNNENLQYDDVCN IA ELHS+IRDAELSGIPAWRII+DPGIGFSKNTKQNLEIL 
Sbjct: 361 PCTMQNNENLQYDDVCNQIALELHSKIRDAELSGIPAWRIIIDPGIGFSKNTKQNLEILR 420

Query: 421 GVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGAN 480
           G+PKIRA IA RSLGLSH PML+GPSRKKFLGEVCS+P+AT+RDPATVAAVTIGVLGGAN
Sbjct: 421 GIPKIRAAIASRSLGLSHVPMLVGPSRKKFLGEVCSRPVATERDPATVAAVTIGVLGGAN 480

Query: 481 IARVHNVRDNVDAVRLCDAMLKE 503
           I RVHNVRDNVDAVR+CDAMLKE
Sbjct: 481 IVRVHNVRDNVDAVRICDAMLKE 503

[Close]

BLAST of CmaCh04G000250 vs. TAIR 10
Match: AT4G30000.1 (Dihydropterin pyrophosphokinase / Dihydropteroate synthase )

HSP 1 Score: 704.1 bits (1816), Expect = 7.9e-203
Identity = 333/484 (68.80%), Postives = 407/484 (84.09%), Query Frame = 0


Query: 22  SSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLYETA 81
           S+F  S+    +EV S E EVVIALGSN+G+R+ NF  ALRL+K+ GI +TRH+CLYETA
Sbjct: 69  SAFSSSATSTTIEVQSTEHEVVIALGSNIGNRMNNFREALRLMKRGGICVTRHSCLYETA 128

Query: 82  PAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRY 141
           P +VT+QP+FLN+AVR VTKLGPHELLS +K IE+ +GR  GIRYGPRP+DLDIL YG+ 
Sbjct: 129 PVHVTDQPRFLNAAVRGVTKLGPHELLSVLKTIERDMGRKDGIRYGPRPLDLDILFYGKM 188

Query: 142 KVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGE 201
           ++ SD L +PHER+WER FVLAPL+DLLGS VD D VA WHSLA   GG+F+ WE++GGE
Sbjct: 189 RISSDKLIIPHERLWERSFVLAPLVDLLGSAVDNDTVAHWHSLAIHPGGIFQAWERLGGE 248

Query: 202 SLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSD 261
           SLIG++G++RVLPIG+ LWD+S KT +MG+LNLTPDSFSDGGKFQ I++AVS+VRSM+S+
Sbjct: 249 SLIGQDGIQRVLPIGDKLWDFSNKTHVMGILNLTPDSFSDGGKFQSIDSAVSRVRSMISE 308

Query: 262 GADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAV 321
           GAD+IDIGAQSTRPMA  IS +EELDRL+PVLEAV GMPEM  KLISVDTF SEVA EA+
Sbjct: 309 GADIIDIGAQSTRPMASRISSQEELDRLLPVLEAVRGMPEMEEKLISVDTFNSEVASEAI 368

Query: 322 KRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASE 381
             GA I+NDVSAG LDP MH+VVA+  VPY+AMHMRGDP TMQN ENLQYDDVC D+ASE
Sbjct: 369 SNGADILNDVSAGTLDPNMHKVVAESGVPYMAMHMRGDPCTMQNKENLQYDDVCKDVASE 428

Query: 382 LHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPML 441
           L+ R+RDAELSGIPAWR+++DPGIGFSK+   NL+I+  +PKIR E+A+RS+ +SHAP+L
Sbjct: 429 LYLRVRDAELSGIPAWRVMIDPGIGFSKSVDHNLDIIMDLPKIREEMAKRSIAVSHAPIL 488

Query: 442 IGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAMLK 501
           +GPSRK+FLG++C +P AT RD ATVA+VT G+LGGANI RVHNVR N DA ++CDAML+
Sbjct: 489 VGPSRKRFLGDICGRPEATDRDAATVASVTAGILGGANIIRVHNVRHNADAAKVCDAMLR 548

Query: 502 EETS 506
              S
Sbjct: 549 RRRS 552

[Close]

BLAST of CmaCh04G000250 vs. TAIR 10
Match: AT4G30000.2 (Dihydropterin pyrophosphokinase / Dihydropteroate synthase )

HSP 1 Score: 692.2 bits (1785), Expect = 3.1e-199
Identity = 328/478 (68.62%), Postives = 402/478 (84.10%), Query Frame = 0


Query: 22  SSFIHSSQDAVLEVCSREQEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLYETA 81
           S+F  S+    +EV S E EVVIALGSN+G+R+ NF  ALRL+K+ GI +TRH+CLYETA
Sbjct: 69  SAFSSSATSTTIEVQSTEHEVVIALGSNIGNRMNNFREALRLMKRGGICVTRHSCLYETA 128

Query: 82  PAYVTNQPQFLNSAVRAVTKLGPHELLSAVKNIEKQLGRTAGIRYGPRPIDLDILLYGRY 141
           P +VT+QP+FLN+AVR VTKLGPHELLS +K IE+ +GR  GIRYGPRP+DLDIL YG+ 
Sbjct: 129 PVHVTDQPRFLNAAVRGVTKLGPHELLSVLKTIERDMGRKDGIRYGPRPLDLDILFYGKM 188

Query: 142 KVHSDTLTVPHERIWERPFVLAPLIDLLGSDVDTDDVACWHSLAADRGGLFELWEKMGGE 201
           ++ SD L +PHER+WER FVLAPL+DLLGS VD D VA WHSLA   GG+F+ WE++GGE
Sbjct: 189 RISSDKLIIPHERLWERSFVLAPLVDLLGSAVDNDTVAHWHSLAIHPGGIFQAWERLGGE 248

Query: 202 SLIGKEGMRRVLPIGNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSD 261
           SLIG++G++RVLPIG+ LWD+S KT +MG+LNLTPDSFSDGGKFQ I++AVS+VRSM+S+
Sbjct: 249 SLIGQDGIQRVLPIGDKLWDFSNKTHVMGILNLTPDSFSDGGKFQSIDSAVSRVRSMISE 308

Query: 262 GADMIDIGAQSTRPMAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAV 321
           GAD+IDIGAQSTRPMA  IS +EELDRL+PVLEAV GMPEM  KLISVDTF SEVA EA+
Sbjct: 309 GADIIDIGAQSTRPMASRISSQEELDRLLPVLEAVRGMPEMEEKLISVDTFNSEVASEAI 368

Query: 322 KRGAHIVNDVSAGQLDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASE 381
             GA I+NDVSAG LDP MH+VVA+  VPY+AMHMRGDP TMQN ENLQYDDVC D+ASE
Sbjct: 369 SNGADILNDVSAGTLDPNMHKVVAESGVPYMAMHMRGDPCTMQNKENLQYDDVCKDVASE 428

Query: 382 LHSRIRDAELSGIPAWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPML 441
           L+ R+RDAELSGIPAWR+++DPGIGFSK+   NL+I+  +PKIR E+A+RS+ +SHAP+L
Sbjct: 429 LYLRVRDAELSGIPAWRVMIDPGIGFSKSVDHNLDIIMDLPKIREEMAKRSIAVSHAPIL 488

Query: 442 IGPSRKKFLGEVCSQPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAM 500
           +GPSRK+FLG++C +P AT RD ATVA+VT G+LGGANI RVHNVR N DA ++  A+
Sbjct: 489 VGPSRKRFLGDICGRPEATDRDAATVASVTAGILGGANIIRVHNVRHNADAAKIDTAV 546

[Close]

BLAST of CmaCh04G000250 vs. TAIR 10
Match: AT1G69190.1 (Dihydropterin pyrophosphokinase / Dihydropteroate synthase )

HSP 1 Score: 661.4 bits (1705), Expect = 5.9e-190
Identity = 313/465 (67.31%), Postives = 394/465 (84.73%), Query Frame = 0


Query: 40  QEVVIALGSNVGDRLQNFNGALRLLKKAGIHITRHACLYETAPAYVTNQPQFLNSAVRAV 99
           +EVVIALGSNVG+R+ NF  ALRL+K  GI +TRH+CLYET P +VT+QP+FLN+A+R V
Sbjct: 12  EEVVIALGSNVGNRMNNFKEALRLMKDYGISVTRHSCLYETEPVHVTDQPRFLNAAIRGV 71

Query: 100 TKLGPHELLSAVKNIEKQLGRTA-GIRYGPRPIDLDILLYGRYKVHSDTLTVPHERIWER 159
           TKL PHELL+ +K IEK++GR   G+RYGPRP+DLDIL YG++K+ SD L +PHERIWER
Sbjct: 72  TKLKPHELLNVLKKIEKEMGREENGLRYGPRPLDLDILFYGKHKIISDKLIIPHERIWER 131

Query: 160 PFVLAPLIDLLGS-DVDTDD-VACWHSLAADRGGLFELWEKMGGESLIGKEG-MRRVLPI 219
           PFVLAPL+DLLG+ D+D D  VA WHSL+   GG+F+ WE++GGESL+GK+G ++RV+PI
Sbjct: 132 PFVLAPLVDLLGTEDIDNDKIVAYWHSLSMHSGGIFQAWERLGGESLLGKDGIIQRVIPI 191

Query: 220 GNNLWDWSCKTSIMGVLNLTPDSFSDGGKFQPIEAAVSQVRSMVSDGADMIDIGAQSTRP 279
           G++LWD+S KT +MG+LNLTPDSFSDGGKFQ ++ AVS+VRSM+S+G D+IDIGAQSTRP
Sbjct: 192 GDHLWDFSKKTYVMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRP 251

Query: 280 MAPMISVEEELDRLVPVLEAVTGMPEMSGKLISVDTFYSEVALEAVKRGAHIVNDVSAGQ 339
           MA  IS +EE+DRL+PVL+ V GM EM GKLISVDTF SEVALEA++ GA I+NDVS G 
Sbjct: 252 MASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGS 311

Query: 340 LDPQMHRVVAQLKVPYIAMHMRGDPSTMQNNENLQYDDVCNDIASELHSRIRDAELSGIP 399
           LD  MH+VVA   VPY+ MHMRGDP TMQN ENL+Y+++C D+A+EL+ R+R+AELSGIP
Sbjct: 312 LDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIP 371

Query: 400 AWRIIVDPGIGFSKNTKQNLEILGGVPKIRAEIARRSLGLSHAPMLIGPSRKKFLGEVCS 459
           AWRI++DPGIGFSK    NL+I+  +PKIR E+A++S+GLSHAP+LIGPSRK+FLG++C 
Sbjct: 372 AWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICG 431

Query: 460 QPIATKRDPATVAAVTIGVLGGANIARVHNVRDNVDAVRLCDAML 501
           +P A++RD ATVA VT G+L GANI RVHNVRDNVDA RLCDAM+
Sbjct: 432 RPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAARLCDAMM 476

The following BLAST results are available for this feature:

BLAST of CmaCh04G000250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04862	2.1e-208	70.41	Folate synthesis bifunctional protein, mitochondrial OS=Pisum sativum OX=3888 GN...	[more]
Q9SZV3	1.1e-201	68.80	Folate synthesis bifunctional protein, mitochondrial OS=Arabidopsis thaliana OX=...	[more]
Q1ENB6	8.3e-189	67.31	Folate synthesis bifunctional protein OS=Arabidopsis thaliana OX=3702 GN=CytHPPK...	[more]
P29251	3.0e-82	39.49	Folic acid synthesis protein fol1 OS=Pneumocystis carinii OX=4754 GN=fol1 PE=1 S...	[more]
Q4LB35	2.4e-71	37.91	Folic acid synthesis protein fol1 OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]

BLAST of CmaCh04G000250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JQH6	1.9e-292	100.00	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1FP77	2.6e-286	98.02	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucurbita moschata OX=366...	[more]
A0A1S4E253	1.4e-263	89.33	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis melo OX=3656 GN=L...	[more]
A0A0A0KP07	2.0e-262	88.93	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Cucumis sativus OX=3659 G...	[more]
A0A6J1DBE6	2.5e-257	87.97	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase OS=Momordica charantia OX=36...	[more]

BLAST of CmaCh04G000250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022990585.1	3.8e-292	100.00	folate synthesis bifunctional protein, mitochondrial-like [Cucurbita maxima]	[more]
XP_023536333.1	2.1e-290	99.01	folate synthesis bifunctional protein, mitochondrial-like [Cucurbita pepo subsp....	[more]
XP_022942566.1	5.4e-286	98.02	folate synthesis bifunctional protein, mitochondrial-like [Cucurbita moschata]	[more]
XP_008456884.1	2.9e-263	89.33	PREDICTED: folate synthesis bifunctional protein, mitochondrial-like [Cucumis me...	[more]
XP_038892551.1	3.2e-262	89.26	folate synthesis bifunctional protein, mitochondrial-like isoform X1 [Benincasa ...	[more]

BLAST of CmaCh04G000250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30000.1	7.9e-203	68.80	Dihydropterin pyrophosphokinase / Dihydropteroate synthase	[more]
AT4G30000.2	3.1e-199	68.62	Dihydropterin pyrophosphokinase / Dihydropteroate synthase	[more]
AT1G69190.1	5.9e-190	67.31	Dihydropterin pyrophosphokinase / Dihydropteroate synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035907	7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK superfamily	GENE3D	3.30.70.560		coord: 39..180 e-value: 5.0E-50 score: 171.7
IPR035907	7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK superfamily	SUPERFAMILY	55083	6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK	coord: 42..171
IPR011005	Dihydropteroate synthase-like	GENE3D	3.20.20.20		coord: 207..504 e-value: 6.6E-105 score: 352.4
IPR011005	Dihydropteroate synthase-like	SUPERFAMILY	51717	Dihydropteroate synthetase-like	coord: 215..501
IPR000489	Pterin-binding domain	PFAM	PF00809	Pterin_bind	coord: 229..484 e-value: 4.1E-84 score: 282.1
IPR000489	Pterin-binding domain	PROSITE	PS00793	DHPS_2	coord: 262..275
IPR000489	Pterin-binding domain	PROSITE	PS00792	DHPS_1	coord: 228..243
IPR000489	Pterin-binding domain	PROSITE	PS50972	PTERIN_BINDING	coord: 226..494 score: 73.339882
IPR006390	Dihydropteroate synthase domain	TIGRFAM	TIGR01496	TIGR01496	coord: 228..498 e-value: 4.7E-82 score: 273.5
IPR006390	Dihydropteroate synthase domain	CDD	cd00739	DHPS	coord: 226..497 e-value: 2.16211E-119 score: 349.213
IPR000550	7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK	PFAM	PF01288	HPPK	coord: 43..168 e-value: 1.6E-33 score: 115.5
IPR000550	7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK	TIGRFAM	TIGR01498	TIGR01498	coord: 42..168 e-value: 1.1E-37 score: 127.0
IPR000550	7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK	PROSITE	PS00794	HPPK	coord: 125..136
IPR000550	7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK	CDD	cd00483	HPPK	coord: 42..168 e-value: 1.56336E-58 score: 187.686
IPR045031	Dihydropteroate synthase	PANTHER	PTHR20941	FOLATE SYNTHESIS PROTEINS	coord: 32..501
None	No IPR available	PANTHER	PTHR20941:SF1	FOLIC ACID SYNTHESIS PROTEIN FOL1	coord: 32..501

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G000250.1	CmaCh04G000250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046656	folic acid biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0046654	tetrahydrofolate biosynthetic process
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
biological_process	GO:0042558	pteridine-containing compound metabolic process
molecular_function	GO:0003848	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004156	dihydropteroate synthase activity
molecular_function	GO:0016301	kinase activity