CmaCh04G000130 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G000130
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionP-loop containing nucleoside triphosphate hydrolases superfamily protein
LocationCma_Chr04: 63077 .. 66772 (-)
RNA-Seq ExpressionCmaCh04G000130
SyntenyCmaCh04G000130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCACCAACACAAAGATTCACAACTTGTATAAATTTGTGATATGGCTTCTCATATCTTCATTCTCACAAACCTCTCCATCTCTCTCTCTCGTTTCATTTCTATAAACCACTTTTTTCTCTCTACAAAATGGAAGGAGGAGAAAGTTGTAGAAGCCACAAAGCAAATAGTGCCAAGGACTCCAATGGTCTCATTGATGTTTTGTTTTCTTGGGAGTTGAGAAATGTGTTCAATCAAAACTTGTATAAACTCAAGGTATGTCTATCTGTTTTTCCTAAAATTCTTGCCTTCCTCTCCTATGCATGTTCACTCATCACAATTCACATTTCTTCCAACGTTTCCATATGTTTATCAGTTCAGGTGAGTTAACATTTCCACTATATAAAGAGTCGCTTTTATAGATTCTTCTATTACTATTTATTTTCGTATTGATGGCTACCTAATACTGTTTATATATATTTTCTGTTTCATGGATATGCATATGAAGTACGAGAGATATCATTCCATTTTCAATGAATATATTGACATATTAGTGGATATTTTAATGAGATTAAATAACTAAGGCCCAATTGTACAAAAATGTTCAGGTGGGGAATATTCCAAAATCATTTGAATCAGAGGAACACTACAGAGGCTCATACTTGTTCCCTTTGTTTGAAGAAACAAGGGCAGAGTTGTGCTCAAGTTTGAAGGCAATTCACAAAGCACCTTCTGCTCAAGTGGTTTCCATTGAAGAGTCAAACACAAAAAGAGGCAAAATCTTGTTCAATGTGAATGTTAGCCCTTGGAGAAGCACTGATGGGAAGGGGCAGCAGCCGTACAAAGCACTTCCAGGCGATATTTTTATAATCTTGGACTCTGATCCTCAAACTACCGATAGTGATTATTTGGAACGCTCAGAATTCAACTGGGCATTTGCTTGGTTGGGACAAATCACCGACAATAGTATCCCTACTCATCTCAAGCTTCATGTTTCTAAAAACATCACAGCTCAAGGTGACATCCTCAAATCAACAACATTTTTCATTGTTTTTCTGATGAATGTGACAACCAACTTGAGAATATGGAAGGCGTTACAATGTTCTGCTGGTGGGGGCATCATTGAGCGTGTTTTGGGCACAACGTGGCTGGTATGTTTTCTATATATATTTGATGCTAAGATTTTTCTTGATAACGATTTTGAATTTAGTATTACTTTTGAAATGGTACAAAGTTAAGTCTCCATTATAATATTTATAAACCTAGCTCTTATTGTGTGAAGTTTTTGAAGTGGATTGTTTTGTAATATGTCAGGGAAACCATCAAAGCTGCACAGAATGCACTCAAAATGATAAAGAAGATCCCACGCAAGATTACCCAACACCACACCTATCTTCATTGAATGAATCCCAAAAAGTTGCAATACAAACTTGTATCGAAAATACTCTCTGCCAACACAAGCCTTCAATAGACCTTATATGGGGCCCACCCGGAACAGGTAAAACCAAAACTACGAGTATATTACTTTGGAGAATCTTAACAATGAAGCACCAAATTAGGACTCTTGCTTGTTCGCCCACAAATGTTGCCATAACAAATCTTGCCTCACAAGTTGTGAAGTTGCTAAAAGACGAGTCTTTTCGTAAAGACCACATCTTTTGCCCTTTGGGACAATTGCTTTTATTTGGGAACAAAGATAGACTTAAAGTTGATTCTCAGCTGGAAGAGATTTATGTGGAGCATAGAGTTGAAAAACTTATCAAGTGTTTAGGTCCAAATGGCTGGAAGTTTCAAATAACATCCATGATAGAGATTCTACAAGGAAGCAAATTTCCAAGATTGAAGAGAATGTTCAAATCCATTGCTTCATCACTTTTAGAATGTGTTCACATTTTAACCACTCACGTCCCTCAAGAAGTCATTATGGAGCATAATTTGAAAAAAATGGAGATTCTTGTTGAGTTGATTGGTGATATTGGGACCCTTCTGAGTGAAGATGATGATAAAGTGAGAGGAACTTTGATTGGTTTGAAGGGTCAATGTGTTTTGGTTTTGCAGACTCTTTTGATGTCTCTTGATCAGGTTGAAGTTCCAAGTAAAGTAAGCAGAAACTCAATCGAGAAGTTTTGTTTTCAGCAAGCTTCTTTGATATTTAGCACAGCTTCAAACTCTTTCAAGTTGAAGAATGTGAAAAAGAATTCACTCAACTTGTTAGTTGTTGATGAAGCTGCTCAATTGAAGGAATGTGAATCACTCATACCTTTGCAACTTCCTGATATAAGGCATGCTATTCTTATTGGCGATGAGTTCCAATTACCAGCAACAGTAAGCAGCAAGGTAATTTATGGTTATCTTATCTTCTTCCTTCTTTGCTCTAACATGAAACACAATTGTTGATTGGCTTTAATTTTGATCAAACCCAATCCTTTCTGTATTAGGTTTCTGAGGCAGCTGGATTTGGTACAAGTCTTTTTGAGAGGCTTAGTGTCTTAGGACACTTCAAGCACCTATTGAACACACAATATAGGATGCATCCGTCTGTGAGCCACTTCCCAAATTCCAAATTCTATGGCAATCAAATTCTGGATGCTTCCATTGTTATGAATAAGCAGCTGTACGAAGAACATTACCTTCCTAGTCCTCTGTTCGGTCCATATTCTTTCATCGATGTTTCTGGTGGACAAGAGGAGAGCAATGATGATGGACAAAGCAAGAAGAACATGTTTGAGGTAGTTGTTGTGGCCCAAATAATACAAATGCTTTACAAAGGTATTGCCAATTTATATTTTTAACATTTTCAATGTTTTGTCTTGCTCACTAAATATCTTGTCACCTACACTTATGTTTTAAAACAAAACAATGGAGACTAAGATATATATTCGAGCGAGAATTTTAAAATATAAAGTATGGTCGTCATTCAAATACAGCATGGTCCAACAAGAAGAAGGATATTAGCATTGGGGTGATATCTCCTTACGCTGCACAAGTTTCATCAATCCAACACAAACTTGGAAGAAAATATGAGAAGGAAGGTTTTACTATAAAAGTGAAGTCTGTTGATGGATTCCAAGGTGGCGAAGAGGATGTGATTATAATATCTACAGTTAGATCCAACCGTGGAAACAACATTGGATTCCTCTCAAGCTCACAAAGAACCAATGTTGCTCTCACGAGAGCTAGGTATAATCTTCTTATTCATTGAGTCAAGTTTAGACATTATTTAGGCCTGACCCAATTGTTTCTATTGTAAATTTTTTCAAGCAATGAACCCAACATGACTTGACTTAACCATAAAATATTTCGGTTAAGTTAGCAAGCTTATAATTTGGAGACTCAACTTGTAATATTTTTTGTCCAGGCATTGTCTTTGGATTGTGGGAGATGCAACAACCTTAGGAAAAAGTAATTCAGAATGGAGAGAAGTTATTAAGGACGCCAAGTCTCGCCAATGCTTCTTTAATGTTGAAGAGGACGAAGAGTTGGGAGATGCAATGAAAATGATGAAGACTTGGCAAATGTCTGATATCAACCAAGAGATTCTCAAACTTGACAATATTTACAACAGTGGTCACAAGAAGAAATCGGATGGGGTTTGATTGCTCTTACACACTTCAATTGTTATTAGAGTGATATGTTTATGAACTTTCAATTAATACGCCTCGTTTAGTTCTGGGGTTTGGCTTATAAGTCATTTTCCAATATTAATTAATAGGGCAGCG

mRNA sequence

TTCACCAACACAAAGATTCACAACTTGTATAAATTTGTGATATGGCTTCTCATATCTTCATTCTCACAAACCTCTCCATCTCTCTCTCTCGTTTCATTTCTATAAACCACTTTTTTCTCTCTACAAAATGGAAGGAGGAGAAAGTTGTAGAAGCCACAAAGCAAATAGTGCCAAGGACTCCAATGGTCTCATTGATGTTTTGTTTTCTTGGGAGTTGAGAAATGTGTTCAATCAAAACTTGTATAAACTCAAGGTGGGGAATATTCCAAAATCATTTGAATCAGAGGAACACTACAGAGGCTCATACTTGTTCCCTTTGTTTGAAGAAACAAGGGCAGAGTTGTGCTCAAGTTTGAAGGCAATTCACAAAGCACCTTCTGCTCAAGTGGTTTCCATTGAAGAGTCAAACACAAAAAGAGGCAAAATCTTGTTCAATGTGAATGTTAGCCCTTGGAGAAGCACTGATGGGAAGGGGCAGCAGCCGTACAAAGCACTTCCAGGCGATATTTTTATAATCTTGGACTCTGATCCTCAAACTACCGATAGTGATTATTTGGAACGCTCAGAATTCAACTGGGCATTTGCTTGGTTGGGACAAATCACCGACAATAGTATCCCTACTCATCTCAAGCTTCATGTTTCTAAAAACATCACAGCTCAAGGTGACATCCTCAAATCAACAACATTTTTCATTGTTTTTCTGATGAATGTGACAACCAACTTGAGAATATGGAAGGCGTTACAATGTTCTGCTGGTGGGGGCATCATTGAGCGTGTTTTGGGCACAACGTGGCTGGGAAACCATCAAAGCTGCACAGAATGCACTCAAAATGATAAAGAAGATCCCACGCAAGATTACCCAACACCACACCTATCTTCATTGAATGAATCCCAAAAAGTTGCAATACAAACTTGTATCGAAAATACTCTCTGCCAACACAAGCCTTCAATAGACCTTATATGGGGCCCACCCGGAACAGGTAAAACCAAAACTACGAGTATATTACTTTGGAGAATCTTAACAATGAAGCACCAAATTAGGACTCTTGCTTGTTCGCCCACAAATGTTGCCATAACAAATCTTGCCTCACAAGTTGTGAAGTTGCTAAAAGACGAGTCTTTTCGTAAAGACCACATCTTTTGCCCTTTGGGACAATTGCTTTTATTTGGGAACAAAGATAGACTTAAAGTTGATTCTCAGCTGGAAGAGATTTATGTGGAGCATAGAGTTGAAAAACTTATCAAGTGTTTAGGTCCAAATGGCTGGAAGTTTCAAATAACATCCATGATAGAGATTCTACAAGGAAGCAAATTTCCAAGATTGAAGAGAATGTTCAAATCCATTGCTTCATCACTTTTAGAATGTGTTCACATTTTAACCACTCACGTCCCTCAAGAAGTCATTATGGAGCATAATTTGAAAAAAATGGAGATTCTTGTTGAGTTGATTGGTGATATTGGGACCCTTCTGAGTGAAGATGATGATAAAGTGAGAGGAACTTTGATTGGTTTGAAGGGTCAATGTGTTTTGGTTTTGCAGACTCTTTTGATGTCTCTTGATCAGGTTGAAGTTCCAAGTAAAGTAAGCAGAAACTCAATCGAGAAGTTTTGTTTTCAGCAAGCTTCTTTGATATTTAGCACAGCTTCAAACTCTTTCAAGTTGAAGAATGTGAAAAAGAATTCACTCAACTTGTTAGTTGTTGATGAAGCTGCTCAATTGAAGGAATGTGAATCACTCATACCTTTGCAACTTCCTGATATAAGGCATGCTATTCTTATTGGCGATGAGTTCCAATTACCAGCAACAGTAAGCAGCAAGGTTTCTGAGGCAGCTGGATTTGGTACAAGTCTTTTTGAGAGGCTTAGTGTCTTAGGACACTTCAAGCACCTATTGAACACACAATATAGGATGCATCCGTCTGTGAGCCACTTCCCAAATTCCAAATTCTATGGCAATCAAATTCTGGATGCTTCCATTGTTATGAATAAGCAGCTGTACGAAGAACATTACCTTCCTAGTCCTCTGTTCGGTCCATATTCTTTCATCGATGTTTCTGGTGGACAAGAGGAGAGCAATGATGATGGACAAAGCAAGAAGAACATGTTTGAGGTAGTTGTTGTGGCCCAAATAATACAAATGCTTTACAAAGCATGGTCCAACAAGAAGAAGGATATTAGCATTGGGGTGATATCTCCTTACGCTGCACAAGTTTCATCAATCCAACACAAACTTGGAAGAAAATATGAGAAGGAAGGTTTTACTATAAAAGTGAAGTCTGTTGATGGATTCCAAGGTGGCGAAGAGGATGTGATTATAATATCTACAGTTAGATCCAACCGTGGAAACAACATTGGATTCCTCTCAAGCTCACAAAGAACCAATGTTGCTCTCACGAGAGCTAGGCATTGTCTTTGGATTGTGGGAGATGCAACAACCTTAGGAAAAAGTAATTCAGAATGGAGAGAAGTTATTAAGGACGCCAAGTCTCGCCAATGCTTCTTTAATGTTGAAGAGGACGAAGAGTTGGGAGATGCAATGAAAATGATGAAGACTTGGCAAATGTCTGATATCAACCAAGAGATTCTCAAACTTGACAATATTTACAACAGTGGTCACAAGAAGAAATCGGATGGGGTTTGATTGCTCTTACACACTTCAATTGTTATTAGAGTGATATGTTTATGAACTTTCAATTAATACGCCTCGTTTAGTTCTGGGGTTTGGCTTATAAGTCATTTTCCAATATTAATTAATAGGGCAGCG

Coding sequence (CDS)

ATGGAAGGAGGAGAAAGTTGTAGAAGCCACAAAGCAAATAGTGCCAAGGACTCCAATGGTCTCATTGATGTTTTGTTTTCTTGGGAGTTGAGAAATGTGTTCAATCAAAACTTGTATAAACTCAAGGTGGGGAATATTCCAAAATCATTTGAATCAGAGGAACACTACAGAGGCTCATACTTGTTCCCTTTGTTTGAAGAAACAAGGGCAGAGTTGTGCTCAAGTTTGAAGGCAATTCACAAAGCACCTTCTGCTCAAGTGGTTTCCATTGAAGAGTCAAACACAAAAAGAGGCAAAATCTTGTTCAATGTGAATGTTAGCCCTTGGAGAAGCACTGATGGGAAGGGGCAGCAGCCGTACAAAGCACTTCCAGGCGATATTTTTATAATCTTGGACTCTGATCCTCAAACTACCGATAGTGATTATTTGGAACGCTCAGAATTCAACTGGGCATTTGCTTGGTTGGGACAAATCACCGACAATAGTATCCCTACTCATCTCAAGCTTCATGTTTCTAAAAACATCACAGCTCAAGGTGACATCCTCAAATCAACAACATTTTTCATTGTTTTTCTGATGAATGTGACAACCAACTTGAGAATATGGAAGGCGTTACAATGTTCTGCTGGTGGGGGCATCATTGAGCGTGTTTTGGGCACAACGTGGCTGGGAAACCATCAAAGCTGCACAGAATGCACTCAAAATGATAAAGAAGATCCCACGCAAGATTACCCAACACCACACCTATCTTCATTGAATGAATCCCAAAAAGTTGCAATACAAACTTGTATCGAAAATACTCTCTGCCAACACAAGCCTTCAATAGACCTTATATGGGGCCCACCCGGAACAGGTAAAACCAAAACTACGAGTATATTACTTTGGAGAATCTTAACAATGAAGCACCAAATTAGGACTCTTGCTTGTTCGCCCACAAATGTTGCCATAACAAATCTTGCCTCACAAGTTGTGAAGTTGCTAAAAGACGAGTCTTTTCGTAAAGACCACATCTTTTGCCCTTTGGGACAATTGCTTTTATTTGGGAACAAAGATAGACTTAAAGTTGATTCTCAGCTGGAAGAGATTTATGTGGAGCATAGAGTTGAAAAACTTATCAAGTGTTTAGGTCCAAATGGCTGGAAGTTTCAAATAACATCCATGATAGAGATTCTACAAGGAAGCAAATTTCCAAGATTGAAGAGAATGTTCAAATCCATTGCTTCATCACTTTTAGAATGTGTTCACATTTTAACCACTCACGTCCCTCAAGAAGTCATTATGGAGCATAATTTGAAAAAAATGGAGATTCTTGTTGAGTTGATTGGTGATATTGGGACCCTTCTGAGTGAAGATGATGATAAAGTGAGAGGAACTTTGATTGGTTTGAAGGGTCAATGTGTTTTGGTTTTGCAGACTCTTTTGATGTCTCTTGATCAGGTTGAAGTTCCAAGTAAAGTAAGCAGAAACTCAATCGAGAAGTTTTGTTTTCAGCAAGCTTCTTTGATATTTAGCACAGCTTCAAACTCTTTCAAGTTGAAGAATGTGAAAAAGAATTCACTCAACTTGTTAGTTGTTGATGAAGCTGCTCAATTGAAGGAATGTGAATCACTCATACCTTTGCAACTTCCTGATATAAGGCATGCTATTCTTATTGGCGATGAGTTCCAATTACCAGCAACAGTAAGCAGCAAGGTTTCTGAGGCAGCTGGATTTGGTACAAGTCTTTTTGAGAGGCTTAGTGTCTTAGGACACTTCAAGCACCTATTGAACACACAATATAGGATGCATCCGTCTGTGAGCCACTTCCCAAATTCCAAATTCTATGGCAATCAAATTCTGGATGCTTCCATTGTTATGAATAAGCAGCTGTACGAAGAACATTACCTTCCTAGTCCTCTGTTCGGTCCATATTCTTTCATCGATGTTTCTGGTGGACAAGAGGAGAGCAATGATGATGGACAAAGCAAGAAGAACATGTTTGAGGTAGTTGTTGTGGCCCAAATAATACAAATGCTTTACAAAGCATGGTCCAACAAGAAGAAGGATATTAGCATTGGGGTGATATCTCCTTACGCTGCACAAGTTTCATCAATCCAACACAAACTTGGAAGAAAATATGAGAAGGAAGGTTTTACTATAAAAGTGAAGTCTGTTGATGGATTCCAAGGTGGCGAAGAGGATGTGATTATAATATCTACAGTTAGATCCAACCGTGGAAACAACATTGGATTCCTCTCAAGCTCACAAAGAACCAATGTTGCTCTCACGAGAGCTAGGCATTGTCTTTGGATTGTGGGAGATGCAACAACCTTAGGAAAAAGTAATTCAGAATGGAGAGAAGTTATTAAGGACGCCAAGTCTCGCCAATGCTTCTTTAATGTTGAAGAGGACGAAGAGTTGGGAGATGCAATGAAAATGATGAAGACTTGGCAAATGTCTGATATCAACCAAGAGATTCTCAAACTTGACAATATTTACAACAGTGGTCACAAGAAGAAATCGGATGGGGTTTGA

Protein sequence

MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSYLFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPYKALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGDILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDPTQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV
Homology
BLAST of CmaCh04G000130 vs. ExPASy Swiss-Prot
Match: Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 1.8e-47
Identity = 163/571 (28.55%), Postives = 274/571 (47.99%), Query Frame = 0

Query: 252  LNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMKH--------- 311
            LN SQ  AI   +       K    LI GPPGTGKTKT   ++   L+ K+         
Sbjct: 1335 LNTSQAEAIVNSVS------KEGFSLIQGPPGTGKTKTILGIIGYFLSTKNASSSNVIKV 1394

Query: 312  --------------QIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFG 371
                          + + L C+P+N A+  +  ++   + D   ++ H F P  QL+  G
Sbjct: 1395 PLEKNSSNTEQLLKKQKILICAPSNAAVDEICLRLKSGVYD---KQGHQFKP--QLVRVG 1454

Query: 372  NKDRLKVDSQLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIAS 431
              D + V   ++++ +E  V+K I   G   ++ +             P L+R F +  +
Sbjct: 1455 RSDVVNV--AIKDLTLEELVDKRI---GERNYEIRTD-----------PELERKFNNAVT 1514

Query: 432  SLLECVHILTTHV--PQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQC 491
               E    L +    P+  +   ++ K+++ +  +  I   L  D D++R          
Sbjct: 1515 KRRELRGKLDSESGNPESPMSTEDISKLQLKIRELSKIINELGRDRDEMR---------- 1574

Query: 492  VLVLQTLLMSLDQVEVPSK-VSRNSIEKFCFQQASLIFSTASNS-FKLKNVKKNSLNLLV 551
                       + V   ++ + R + +      + +I ST S S   +        + ++
Sbjct: 1575 ---------EKNSVNYRNRDLDRRNAQAHILAVSDIICSTLSGSAHDVLATMGIKFDTVI 1634

Query: 552  VDEAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGH 611
            +DEA Q  E  S+IPL+    +  I++GD  QLP TV S  +    +  SLF R+     
Sbjct: 1635 IDEACQCTELSSIIPLRYGG-KRCIMVGDPNQLPPTVLSGAASNFKYNQSLFVRME-KNS 1694

Query: 612  FKHLLNTQYRMHPSVSHFPNSKFYGNQILD--ASIVMNKQLYEEHYLPSPLFGPYSFIDV 671
              +LL+ QYRMHPS+S FP+S+FY  ++ D     ++NK+ +  H L  PL  PY F D+
Sbjct: 1695 SPYLLDVQYRMHPSISKFPSSEFYQGRLKDGPGMDILNKRPW--HQL-EPL-APYKFFDI 1754

Query: 672  SGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDI-SIGVISPYAAQVSSIQHK 731
              G++E N    S  NM E+ V  +++  L++ + NK      IG+ISPY  Q+  ++ +
Sbjct: 1755 ISGRQEQNAKTMSYTNMEEIRVAIELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRKE 1814

Query: 732  LGRKY-EKEGFTIKVKSVDGFQGGEEDVIIISTVRS-NRGNNIGFLSSSQRTNVALTRAR 791
              R +      +I   ++DGFQG E+++I+IS VR+ +  +++GFL   +R NVALTRA+
Sbjct: 1815 FARYFGGMINKSIDFNTIDGFQGQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRAK 1852

BLAST of CmaCh04G000130 vs. ExPASy Swiss-Prot
Match: B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.2e-46
Identity = 120/335 (35.82%), Postives = 184/335 (54.93%), Query Frame = 0

Query: 489 NSIEKFCFQQASLIFSTASNSFKLKNVKKN-SLNLLVVDEAAQLKECESLIPLQLPDIRH 548
           +SI     ++A+++F+T S S      K N   +++++DEAAQ  E  +LIPL     + 
Sbjct: 452 DSIRTAILEEAAIVFATLSFSGSALLAKSNRGFDVVIIDEAAQAVEPATLIPL-ATRCKQ 511

Query: 549 AILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVSHFPNSKF 608
             L+GD  QLPATV S V++ +G+GTS+FERL   G+   +L TQYRMHP +  FP+ +F
Sbjct: 512 VFLVGDPKQLPATVISTVAQDSGYGTSMFERLQKAGYPVKMLKTQYRMHPEIRSFPSKQF 571

Query: 609 YGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQ-SKKNMFEVVVVA 668
           Y   + D S +  +   + H      FGP+ F D+  G+E  +     S+ N+ EV  V 
Sbjct: 572 YEGALEDGSDIEAQTTRDWH--KYRCFGPFCFFDIHEGKESQHPGATGSRVNLDEVEFVL 631

Query: 669 QIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEG-FTIKVKSVDGFQGGE 728
            I   L   +   K    + +ISPY  QV + + +    +  E    + + +VDGFQG E
Sbjct: 632 LIYHRLVTMYPELKSSSQLAIISPYNYQVKTFKDRFKEMFGTEAEKVVDINTVDGFQGRE 691

Query: 729 EDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWREVIKDA 788
           +DV I S VR+N    IGFLS+S+R NV +TRA+  + +VG A TL KS+  W+ +I+ A
Sbjct: 692 KDVAIFSCVRANENGQIGFLSNSRRMNVGITRAKSSVLVVGSAATL-KSDPLWKNLIESA 751

Query: 789 KSRQCFFNVEE------DEELGDAMKMMKTWQMSD 815
           + R   F V +       EE  + MK+ +  ++ D
Sbjct: 752 EQRNRLFKVSKPLNNFFSEENLETMKLTEDMEIPD 782

BLAST of CmaCh04G000130 vs. ExPASy Swiss-Prot
Match: Q92355 (Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen1 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 9.9e-46
Identity = 172/610 (28.20%), Postives = 291/610 (47.70%), Query Frame = 0

Query: 232  CTQNDKEDPTQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTS 291
            C+Q  K   ++         +NE Q  AI   ++N          LI GPPGTGKTKT  
Sbjct: 1110 CSQPVKHSSSEIKAAMKRYQVNEPQAKAIMCALDNN------GFTLIQGPPGTGKTKTII 1169

Query: 292  ILLWRIL----------------TMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKD 351
             ++  +L                + + + + L C+P+N A+  +   +++L +       
Sbjct: 1170 GIISALLVDLSRYHITRPNQQSKSTESKQQILLCAPSNAAVDEV---LLRLKRGFLLENG 1229

Query: 352  HIFCPLGQLLLFGNKDRLKVDSQLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSK 411
              + P  +++  GN + + V   + ++ +E++ EK                ++E+ QG+ 
Sbjct: 1230 EKYIP--RVVRIGNPETINV--SVRDLSLEYQTEK---------------QLLEVNQGAI 1289

Query: 412  FPRLKRMFKSIASSLLECVHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKV 471
                 +       +  +C+  +          E   K++++  ++  D  +L  E  +K+
Sbjct: 1290 DLGSLQELTRWRDTFYDCIQKI----------EELEKQIDVARDVAEDTKSLGKELQNKI 1349

Query: 472  RGTLIGLKGQCVLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNV 531
                  L  Q V  LQ+   + ++ EV   + R   +K   +QA ++ +T S S     V
Sbjct: 1350 NEK--NLAEQKVEELQSQSFTKNK-EV--DLLRKKAQKAILKQADVVCATLSGSGH-DLV 1409

Query: 532  KKNSLNL--LVVDEAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGT 591
              +SLN   +++DEAAQ  E +++IPL+    +  IL+GD  QLP TV SK + +  +  
Sbjct: 1410 AHSSLNFSTVIIDEAAQAVELDTIIPLRY-GAKKCILVGDPNQLPPTVLSKKAASLNYSQ 1469

Query: 592  SLFERLSV-LGHFKHLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSP 651
            SLF R+     +   LL+ QYRMHP +SHFP+ KFY +++ D   +  K     H   +P
Sbjct: 1470 SLFVRIQKNFSNQMCLLSIQYRMHPDISHFPSKKFYDSRLEDGDNMAEKTQQVWHV--NP 1529

Query: 652  LFGPYSFIDVSGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYA 711
             F  Y   DV G +  SN    S  N+ EV  +  ++  L   + +      IGVI+PY 
Sbjct: 1530 KFTQYRLFDVRGKERTSNT--MSTYNLEEVEYLVNMVDELLNKFPDVNFTGRIGVITPYR 1589

Query: 712  AQVSSIQHKLGRKYEKEGF-TIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRT 771
            +Q+  ++     KY K    TI +++VDGFQG E+D+I  S V+S   + IGFL   +R 
Sbjct: 1590 SQLHELRRAFKVKYGKSFMSTIDIQTVDGFQGQEKDIIFFSCVKSYSKHGIGFLRDFRRL 1649

Query: 772  NVALTRARHCLWIVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQ 822
            NVALTRAR  L I+G+  TL K++  W  ++ DA SR+   +   D E G  + + +T +
Sbjct: 1650 NVALTRARSSLLIIGNMETL-KTDDLWGSLVDDALSRKLVESPHIDSE-GRLITISRTSE 1668

BLAST of CmaCh04G000130 vs. ExPASy Swiss-Prot
Match: A2AKX3 (Probable helicase senataxin OS=Mus musculus OX=10090 GN=Setx PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 3.2e-44
Identity = 184/662 (27.79%), Postives = 304/662 (45.92%), Query Frame = 0

Query: 182  LKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDPT 241
            +K+ T  IV    VTT  ++      S+   +   VL      N      CT+ D    T
Sbjct: 1847 VKNLTRCIVISSLVTTQRKLKAMSLLSSRNQLARAVL------NPNPMDFCTK-DLLTTT 1906

Query: 242  QDYPTPHLSSLNESQKVAIQTCIENTLCQHKPS---IDLIWGPPGTGKTKTTSILLWRIL 301
             +    +L   NE QK AI+T     + +H PS   I LI GPPGTGK+KT   LL+R+L
Sbjct: 1907 SERIVAYLKDFNEDQKKAIETAY--AMVKHSPSVAKICLIHGPPGTGKSKTIVGLLYRLL 1966

Query: 302  TMKH--------------QIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQL 361
            T                 Q R L C+P+N A+  L  +++   K++   K +   PLG  
Sbjct: 1967 TENQRKGHSDENFNAKIKQNRVLVCAPSNAAVDELMKKIILEFKEKCKDKKN---PLGNC 2026

Query: 362  LLFGNKDRLKVDSQLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFK 421
               G+ + +++  +              K +     KF + S +         R+K+   
Sbjct: 2027 ---GDINLVRLGPE--------------KSINTEVLKFSLDSQVN-------HRMKKDLP 2086

Query: 422  SIASSLLECVHILTTHVPQEVIMEHNLKK--MEILVELIGDIGTLLSEDDDKVRGTLIGL 481
            S    +L    IL   +  E+  +  L +   E+  + + +   ++S++  ++   +  +
Sbjct: 2087 SHIQEMLRRKEILDAQL-DELSRQRALCRGGREMQRQELDEHIAIVSKERQELASKIKEV 2146

Query: 482  KGQCVLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQ-QASLIFSTASNSFKLKNVKKNSLN 541
            +G+      T+++    +      S   + +  F+ Q  + FS                 
Sbjct: 2147 QGRPQRAQNTIILESHVICCTLSTSGGLLLESAFRGQGGVPFS----------------- 2206

Query: 542  LLVVDEAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERL-- 601
             ++VDEA Q  E E+L PL +      IL+GD  QLP TV S  ++  G+  S+  R   
Sbjct: 2207 CVIVDEAGQSCEVETLSPL-IHRCNKLILVGDPKQLPPTVISMKAQEYGYDQSMMARFCK 2266

Query: 602  --------SVLGHFKHL-LNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLP 661
                    +++G    L L  QYRMHP +  FP++  Y   +    +  + +   E    
Sbjct: 2267 LLEENVEQNMIGRLPVLQLTIQYRMHPDICLFPSNYVYNKNLKTNRLTESIRCSSEW--- 2326

Query: 662  SPLFGPYSFIDVSGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDIS---IGV 721
               F PY   DV  G E  ++D  S  N+ E+ +V +II+++      K+KDIS   IG+
Sbjct: 2327 --PFQPYLVFDVGDGSERRDND--SYINVQEIKLVMEIIKLI----KEKRKDISFRNIGI 2386

Query: 722  ISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDGFQGGEEDVIIISTVRSNR-GNNIGFLS 781
            I+ Y AQ + IQ  L ++++K+G   +V +VD FQG ++D II++ VR++    +IGFL+
Sbjct: 2387 ITHYKAQKTMIQKDLEKEFDKKG-PAEVDTVDAFQGRQKDCIIVTCVRASAVQGSIGFLA 2440

Query: 782  SSQRTNVALTRARHCLWIVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKM 809
            S QR NV +TRA++ L+I+G   TL   N  W E+I+DA+ R       +     DAMK+
Sbjct: 2447 SLQRLNVTITRAKYSLFILGHLRTL-MENQHWYELIQDAQKRGAIIKTSDPNYRHDAMKI 2440

BLAST of CmaCh04G000130 vs. ExPASy Swiss-Prot
Match: O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.8e-42
Identity = 179/626 (28.59%), Postives = 294/626 (46.96%), Query Frame = 0

Query: 192  LMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDPTQDYPTPHLSS 251
            L N TT+LR + AL+      + +R+L         + T    N  +D  Q     +   
Sbjct: 1220 LFNATTSLREFAALKSLRHLPLSQRILDA-------NVTRLPSNFTDDKKQKIMKSY--G 1279

Query: 252  LNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMKHQ-------- 311
            +NE Q  AI     N          LI GPPGTGKTKT   ++  +LT   Q        
Sbjct: 1280 VNEPQAYAIYASSVN------DGFTLIQGPPGTGKTKTILGMIGAVLTSSSQGLQFNVPG 1339

Query: 312  -------IRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVD 371
                    + L C+P+N AI  +  ++   + D    K   F P  +++  G  D + V 
Sbjct: 1340 QTRKTSKNKILICAPSNAAIDEILLRIKAGVYDHEGIK---FFP--KVIRVGFGDSISVH 1399

Query: 372  SQLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSI---ASSLLEC 431
            +  +E  +E   E++IK       + ++T++ +  + +     ++ + SI     SL E 
Sbjct: 1400 A--KEFTLE---EQMIK-------QMELTNLKKDQEANNSSDTRKKYDSIIKKRDSLRED 1459

Query: 432  VHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTL 491
            +    +      I+E  L+++     ++       S DD + R              ++ 
Sbjct: 1460 LEKFRSTGKNSSILEAQLREITKQKNMLEQ-----SLDDMRER-------------QRST 1519

Query: 492  LMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNS-FKLKNVKKNSLNLLVVDEAAQLK 551
              +LD       V +  I+    Q+A ++ +T S S  +L      +   +++DEAAQ  
Sbjct: 1520 NRNLD-------VLKKQIQNQLLQEADIVCATLSASGHELLLNAGLTFRTVIIDEAAQAV 1579

Query: 552  ECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKH----- 611
            E  S+IPL+       +++GD  QLP TV SK S   G+  SL+ R+     FK      
Sbjct: 1580 ELSSIIPLKY-GCESCVMVGDPNQLPPTVLSKTSAKFGYSQSLYVRM-----FKQHNESA 1639

Query: 612  -LLNTQYRMHPSVSHFPNSKFYGNQILDA---SIVMNKQLYEEHYLPSPLFGPYSFIDVS 671
             LL+ QYRM+P +S FP+  FY +++LD    S V ++  +E+     P  G Y F +V 
Sbjct: 1640 CLLSIQYRMNPEISRFPSKFFYNSKLLDGPNMSAVTSRPWHED-----PQLGIYRFFNVH 1699

Query: 672  GGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLG 731
            G +  SN   +S  N+ E   +  + + L + + N   +  IGV++PY +QV  ++ +  
Sbjct: 1700 GTEAFSN--SKSLYNVEEASFILLLYERLIQCYLNIDFEGKIGVVTPYRSQVQQLRSQFQ 1759

Query: 732  RKYEKEGFT-IKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCL 789
            RKY    F  + + +VDGFQG E+D+II S VRS+    IGFL   +R NVALTRA+  L
Sbjct: 1760 RKYGSIIFKHLDIHTVDGFQGQEKDIIIFSCVRSSMSGGIGFLQDLRRLNVALTRAKSSL 1774

BLAST of CmaCh04G000130 vs. ExPASy TrEMBL
Match: A0A6J1JC41 (helicase sen1-like OS=Cucurbita maxima OX=3661 GN=LOC111485435 PE=4 SV=1)

HSP 1 Score: 1676.0 bits (4339), Expect = 0.0e+00
Identity = 837/837 (100.00%), Postives = 837/837 (100.00%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY
Sbjct: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120
           LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY
Sbjct: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120

Query: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180
           KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD
Sbjct: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180

Query: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240
           ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP
Sbjct: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240

Query: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300
           TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM
Sbjct: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300

Query: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360
           KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE
Sbjct: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360

Query: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420
           EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH
Sbjct: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420

Query: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480
           VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV
Sbjct: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480

Query: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540
           EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL
Sbjct: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540

Query: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600
           QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS
Sbjct: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600

Query: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660
           HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF
Sbjct: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660

Query: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDG 720
           EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDG
Sbjct: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDG 720

Query: 721 FQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWRE 780
           FQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWRE
Sbjct: 721 FQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWRE 780

Query: 781 VIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV 838
           VIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV
Sbjct: 781 VIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV 837

BLAST of CmaCh04G000130 vs. ExPASy TrEMBL
Match: A0A6J1FQ96 (helicase SEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111447261 PE=4 SV=1)

HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 810/834 (97.12%), Postives = 818/834 (98.08%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCR HK +SAKDSNGLIDVLFSWEL NVFNQNLYKLKVGNIPKSFESEEHYRGSY
Sbjct: 1   MEGGESCRRHKPSSAKDSNGLIDVLFSWELTNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120
           LFPL EETRAELCSSLKAIHKAPSAQVVSIEESN KRGKILFNVNVSPWRSTDGKGQQPY
Sbjct: 61  LFPLLEETRAELCSSLKAIHKAPSAQVVSIEESNAKRGKILFNVNVSPWRSTDGKGQQPY 120

Query: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180
           KALPG IFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHL LHVSKNITAQGD
Sbjct: 121 KALPGHIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLNLHVSKNITAQGD 180

Query: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240
           IL+STT FIVFLMNVTTNLRIWKALQCSAGGGII RVLGTTWLGNHQSCTECTQND+EDP
Sbjct: 181 ILESTTLFIVFLMNVTTNLRIWKALQCSAGGGIIGRVLGTTWLGNHQSCTECTQNDQEDP 240

Query: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300
           TQDYPTPH SSLNESQKVAI+TCI+NTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM
Sbjct: 241 TQDYPTPHPSSLNESQKVAIETCIQNTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300

Query: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360
           KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE
Sbjct: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360

Query: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420
           EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQG KFPRLKRMFKSIASSLLECVHILTTH
Sbjct: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGCKFPRLKRMFKSIASSLLECVHILTTH 420

Query: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480
           VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV
Sbjct: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480

Query: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540
           EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL
Sbjct: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540

Query: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600
           QLPDIRHAILIGDEFQLPAT+SSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS
Sbjct: 541 QLPDIRHAILIGDEFQLPATISSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600

Query: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660
           HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFI+VSGGQEESNDDGQSKKNMF
Sbjct: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFINVSGGQEESNDDGQSKKNMF 660

Query: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYE-KEGFTIKVKSVD 720
           EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYE KEGFTIKVKSVD
Sbjct: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKKEGFTIKVKSVD 720

Query: 721 GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 780
           GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR
Sbjct: 721 GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 780

Query: 781 EVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKK 834
           EVIKDAKSRQC FNVEED+ELGDAMKMMKT QMSDINQEIL LDNIYNS HKKK
Sbjct: 781 EVIKDAKSRQCLFNVEEDKELGDAMKMMKTSQMSDINQEILNLDNIYNSDHKKK 834

BLAST of CmaCh04G000130 vs. ExPASy TrEMBL
Match: A0A1S3C4A0 (helicase SEN1-like OS=Cucumis melo OX=3656 GN=LOC103496680 PE=4 SV=1)

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 613/845 (72.54%), Postives = 716/845 (84.73%), Query Frame = 0

Query: 2   EGG-ESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 61
           EGG  SCRS+KA++ K+SNGLID LFSW+  NVFNQN YKLKV  IPKSFE+EE Y+ SY
Sbjct: 4   EGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASY 63

Query: 62  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTD-GKGQQP 121
           +FPL EETRAELCS+LK I KAP +QV+SIE +NTK+GKILFNVNVS WR+ D GKGQQP
Sbjct: 64  IFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFNVNVSSWRNRDGGKGQQP 123

Query: 122 YKALPGDIFIILDSDPQTTDSDYLER-SEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQ 181
           YK+LPGDIF+ILD+DPQT  SDYLE+ S+ NWAFAWLGQ+ DN+ PTHL LH+S N+   
Sbjct: 124 YKSLPGDIFVILDTDPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM--- 183

Query: 182 GDILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKE 241
            D L ST  FIVFLMN+TTNLRIWK LQCS+ GGI++ +LGTT + N ++C +C  ND E
Sbjct: 184 -DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDN-KTCKQCNGNDGE 243

Query: 242 D-PTQDYPTPHLS--SLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLW 301
           D  TQ++PT  L   SLNESQ+VAI++CI+  +CQHKPSI+LIWGPPGTGKTKTTSILLW
Sbjct: 244 DYSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLW 303

Query: 302 RILTMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKV 361
           +ILT+ HQIRTLAC+PTNVAITNLASQVV LLK +S  K+ +FCPLG+LLLFGNKDRLK 
Sbjct: 304 KILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKF 363

Query: 362 DS--QLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLEC 421
           DS  QL++IY++ RVEKL KCLG +G KFQITSMI I Q +K  ++KRMFK  AS LL+C
Sbjct: 364 DSHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDC 423

Query: 422 VHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLS-----EDDDKVRGTLIGLKGQCVL 481
           VHI TTH+P++VIMEHN KK+EILV  I DIGTLLS     +DDDK+   LI LK  C+L
Sbjct: 424 VHIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDKMGEALIDLKCHCLL 483

Query: 482 VLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEA 541
           VL+TLL+SLD++EVPSK+S+NSIEKFCFQ+ASLIFSTASNSFKL +VKKNSLNL+VVDEA
Sbjct: 484 VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA 543

Query: 542 AQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHL 601
           AQLKECESL+PLQL  I HA+L+GDEFQLPAT+ SK+ E A FG SL+ERLS++G+ KHL
Sbjct: 544 AQLKECESLVPLQLQHITHALLVGDEFQLPATIKSKICEGAKFGRSLYERLSLMGYSKHL 603

Query: 602 LNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEE 661
           L+TQYRMHP VS+FPNSKFYGN+I+DASIVMNK  YE++YLPSPLFGPYSFI+V GGQEE
Sbjct: 604 LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKG-YEKNYLPSPLFGPYSFINVCGGQEE 663

Query: 662 SNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEK 721
           SN DGQSKKN  EV+VV QIIQMLYKAW   K DISIGVISPY AQVSSIQ KLGRKYEK
Sbjct: 664 SNGDGQSKKNTVEVIVVTQIIQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEK 723

Query: 722 ---EGFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIV 781
              EGF +KVKS+DGFQGGEEDVIIISTVRSN G+NIGFLS+ QRTNVALTRAR CLWIV
Sbjct: 724 NNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNNGHNIGFLSNKQRTNVALTRARFCLWIV 783

Query: 782 GDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNI 831
           GDA TLGKSNSEWR++I DAK+RQCFFNVEE++EL + M+M+KTWQ+ DI QEILKLDNI
Sbjct: 784 GDAKTLGKSNSEWRDIINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNI 842

BLAST of CmaCh04G000130 vs. ExPASy TrEMBL
Match: A0A0A0KMQ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G292250 PE=4 SV=1)

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 617/845 (73.02%), Postives = 707/845 (83.67%), Query Frame = 0

Query: 2   EGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSYL 61
           EGG    S+K ++ KDSNGLID LFSW+  NVFNQN+YK KV  IPKSFE+EE Y+GSY+
Sbjct: 4   EGG---LSNKGSNHKDSNGLIDALFSWDFNNVFNQNIYKPKVRKIPKSFETEEQYKGSYI 63

Query: 62  FPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRST-DGKGQQPY 121
           FPL EETRAELCS+LK I KAP +QV+SIE SNTK+ KILFNVNVS WR+T  GKGQQPY
Sbjct: 64  FPLLEETRAELCSNLKTIQKAPFSQVISIETSNTKKDKILFNVNVSSWRNTYGGKGQQPY 123

Query: 122 KALPGDIFIILDSDPQTTDSDYLER-SEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQG 181
           K+LPGD F+ILD DPQT  SDYLE+ S+ NWAFAWLGQ+ DN+ PTHLKLH+S ++    
Sbjct: 124 KSLPGDFFVILDVDPQTITSDYLEKSSKLNWAFAWLGQVNDNNTPTHLKLHISNSM---- 183

Query: 182 DILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTEC-TQNDKE 241
           D LKST  FIVFLMN+TTNLRIWK LQCS+ GGI++ VLGT  + N ++C +C  Q D E
Sbjct: 184 DQLKSTPLFIVFLMNLTTNLRIWKTLQCSSSGGIVKHVLGTMSMDN-KTCKQCNNQTDGE 243

Query: 242 DPTQDYPTPHLS--SLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWR 301
           D T+D  T  L+  SLNESQ+VAI++CI    CQHKPSI+LIWGPPGTGKTKTTSILLW+
Sbjct: 244 DSTEDMATLRLASLSLNESQRVAIESCIRKVKCQHKPSIELIWGPPGTGKTKTTSILLWK 303

Query: 302 ILTMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVD 361
           IL + HQIRTLAC+PTNVAITNLASQV+KLLK +S  ++ IFCPLG+LLLFGNKDRLK D
Sbjct: 304 ILAINHQIRTLACAPTNVAITNLASQVLKLLKHDSLSRNAIFCPLGELLLFGNKDRLKFD 363

Query: 362 --SQLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECV 421
              QLE+IY++ RVEKL KCLG  G KFQI+SMI I Q +K  +LKRMFKS  SSLLECV
Sbjct: 364 YSHQLEDIYLDRRVEKLFKCLGQYGLKFQISSMIGIFQENKLSKLKRMFKSNVSSLLECV 423

Query: 422 HILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLS------EDDDKVRGTLIGLKGQCVL 481
           HI TTH+PQ+VIMEHN KK+EILV  I DIGTLLS      +DDD +   LI LK   +L
Sbjct: 424 HIFTTHIPQQVIMEHNWKKLEILVGFICDIGTLLSKNNYNYDDDDTMGEALIDLKCHFLL 483

Query: 482 VLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEA 541
           VL+TLL+SLD++EVPSK+S+NSIEKFCFQ+ASLIFST SNSFKL +VKKNSLNL+VVDEA
Sbjct: 484 VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTTSNSFKLNSVKKNSLNLVVVDEA 543

Query: 542 AQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHL 601
           AQLKECESLIPLQLP I HAIL+GDEFQLPATV SKV E A FG SL+ERLS++G+ KHL
Sbjct: 544 AQLKECESLIPLQLPHISHAILVGDEFQLPATVKSKVCERAKFGRSLYERLSLIGYSKHL 603

Query: 602 LNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEE 661
           L+TQYRMHP VS+FPNSKFYGN+I+DASIVMNK+ YE+ YLPSPLFGPYSFI+V GG+EE
Sbjct: 604 LDTQYRMHPLVSYFPNSKFYGNKIMDASIVMNKE-YEKEYLPSPLFGPYSFINVCGGEEE 663

Query: 662 SNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEK 721
           SN DGQSKKNM EV VV QIIQMLYKAW   KKDISIG+ISPY AQVSSIQ KLGRKYEK
Sbjct: 664 SNGDGQSKKNMVEVTVVTQIIQMLYKAWCKNKKDISIGIISPYNAQVSSIQEKLGRKYEK 723

Query: 722 ---EGFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIV 781
              EGF +KVKS+DGFQGGEEDVIIISTVRSN G+NIGFLS+ QRTNVALTRAR CLWIV
Sbjct: 724 KNNEGFGVKVKSIDGFQGGEEDVIIISTVRSNNGHNIGFLSNKQRTNVALTRARFCLWIV 783

Query: 782 GDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNI 831
           GDA TLGKSNSEWR+VI DAK+R+CFFNVEE++EL + M+MMKTWQMSDI QEILKLDNI
Sbjct: 784 GDAKTLGKSNSEWRDVIDDAKTRRCFFNVEENKELANEMRMMKTWQMSDIKQEILKLDNI 839

BLAST of CmaCh04G000130 vs. ExPASy TrEMBL
Match: A0A6J1DR27 (helicase SEN1-like OS=Momordica charantia OX=3673 GN=LOC111023039 PE=4 SV=1)

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 617/842 (73.28%), Postives = 714/842 (84.80%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCRS KAN AKD NGLID+LFSWELR+VFNQN YKLKVG IPKSFESE HYR SY
Sbjct: 1   MEGGESCRSKKAN-AKDCNGLIDILFSWELRHVFNQNFYKLKVGKIPKSFESEHHYRSSY 60

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRG-KILFNVNVSPWRSTDGKG-QQ 120
           ++PL EETRA+LCSSLK IHKAPSAQ++SIE+    +G KILFNVN+S WR  +GKG QQ
Sbjct: 61  IYPLLEETRADLCSSLKTIHKAPSAQLLSIEKEPKPKGPKILFNVNLSSWRINNGKGQQQ 120

Query: 121 PYKALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIP-THLKLHVSKNITA 180
            Y+ LPGDIF+ILD DPQTT    LE S   WAFAWLG I DN+ P THLKL+VSK+I+A
Sbjct: 121 SYRPLPGDIFVILDIDPQTTTD--LECSN-TWAFAWLGHIIDNNAPTTHLKLYVSKDISA 180

Query: 181 QGDILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECT-QND 240
           +GDI ++TT FIVFLMNVTTNLRIWKALQ SA  GI++RVLG+T    +Q+C EC+ +N 
Sbjct: 181 EGDIHQTTTLFIVFLMNVTTNLRIWKALQSSADVGIVKRVLGST---GNQTCKECSEENS 240

Query: 241 KEDPTQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWR 300
            E+PT        SSLNESQK+AI++C+ N LCQHKPSIDLIWGPPGTGKTKTTS+LL +
Sbjct: 241 IENPT---TLSSSSSLNESQKLAIESCVHNVLCQHKPSIDLIWGPPGTGKTKTTSMLLLK 300

Query: 301 ILTMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDH-IFCPLGQLLLFGNKDRLKV 360
           IL M HQIRTLAC+PTN+AITNLAS+VVKLLK+E+F K++ ++ PLG+LLLFGNKDRLK+
Sbjct: 301 ILRMTHQIRTLACAPTNIAITNLASRVVKLLKEEAFSKEYKLYSPLGELLLFGNKDRLKI 360

Query: 361 DSQLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVH 420
           DS+LEE+Y+E+RVE L+KCLG NGWKFQITSMIE L+  K  R    FKSIA +L EC++
Sbjct: 361 DSELEEVYLENRVESLVKCLGKNGWKFQITSMIEFLEEGK--RRIHHFKSIAPALHECLY 420

Query: 421 ILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDK--VRGTLIGLKGQCVLVLQTL 480
            L THVP +VI+EHNLKKMEILV+L+ D GTLL ++D K  VR  L  LK +C+L L+ L
Sbjct: 421 SLATHVPPQVILEHNLKKMEILVKLVDDFGTLLPQNDSKVSVRENLADLKRKCLLGLKNL 480

Query: 481 LMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKE 540
           L+SL+Q+EVPSKVSRNSIEKFCFQ+ASLIFSTASNSFKL +VKKNS++LLV+DEAAQLKE
Sbjct: 481 LVSLEQIEVPSKVSRNSIEKFCFQKASLIFSTASNSFKLNSVKKNSVDLLVIDEAAQLKE 540

Query: 541 CESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQY 600
           CESLIPLQ+P+IRHAILIGDEFQLPA VSSKV +AAG+G SLFERLS+LG+  HLL+TQY
Sbjct: 541 CESLIPLQVPNIRHAILIGDEFQLPAIVSSKVCDAAGYGRSLFERLSLLGYSTHLLDTQY 600

Query: 601 RMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDG 660
           RMHP VS FPNSKFYGNQILDASIVM+KQ YE  YL  P+FGPYSFI V GGQEESN DG
Sbjct: 601 RMHPCVSFFPNSKFYGNQILDASIVMDKQ-YERSYLAGPMFGPYSFIHVCGGQEESNRDG 660

Query: 661 QSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEK----E 720
           QSKKNM EVVVV QIIQMLYKAW   K+D+SIGVISPYAAQVS+IQ+K+G KYEK    E
Sbjct: 661 QSKKNMVEVVVVTQIIQMLYKAWCKSKRDVSIGVISPYAAQVSAIQNKVGGKYEKNIINE 720

Query: 721 GFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDAT 780
           GFT+KVKSVDGFQGGEEDVIIISTVRSN  N+IGFLSSSQRTNVALTRAR+CLWIVGDA 
Sbjct: 721 GFTVKVKSVDGFQGGEEDVIIISTVRSNDRNDIGFLSSSQRTNVALTRARYCLWIVGDAE 780

Query: 781 TLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSG 832
           TL KSNSEW++VI DAKSR CFFNV+ED+EL DAM+M KTWQMS+I +E+LKLDNIYN  
Sbjct: 781 TLEKSNSEWKDVIDDAKSRHCFFNVDEDKELADAMRMTKTWQMSNIKEELLKLDNIYNKD 829

BLAST of CmaCh04G000130 vs. NCBI nr
Match: XP_022988087.1 (helicase sen1-like [Cucurbita maxima])

HSP 1 Score: 1676.0 bits (4339), Expect = 0.0e+00
Identity = 837/837 (100.00%), Postives = 837/837 (100.00%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY
Sbjct: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120
           LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY
Sbjct: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120

Query: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180
           KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD
Sbjct: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180

Query: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240
           ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP
Sbjct: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240

Query: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300
           TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM
Sbjct: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300

Query: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360
           KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE
Sbjct: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360

Query: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420
           EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH
Sbjct: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420

Query: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480
           VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV
Sbjct: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480

Query: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540
           EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL
Sbjct: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540

Query: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600
           QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS
Sbjct: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600

Query: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660
           HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF
Sbjct: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660

Query: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDG 720
           EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDG
Sbjct: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDG 720

Query: 721 FQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWRE 780
           FQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWRE
Sbjct: 721 FQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWRE 780

Query: 781 VIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV 838
           VIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV
Sbjct: 781 VIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV 837

BLAST of CmaCh04G000130 vs. NCBI nr
Match: XP_023517650.1 (helicase SEN1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1624.8 bits (4206), Expect = 0.0e+00
Identity = 814/838 (97.14%), Postives = 823/838 (98.21%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCRSHKA+SAKDSNGLIDVLFSWEL NVFNQNLYKLKVGNIPKSFESEEHYRGSY
Sbjct: 29  MEGGESCRSHKASSAKDSNGLIDVLFSWELTNVFNQNLYKLKVGNIPKSFESEEHYRGSY 88

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120
           LFPL EETRAELCSSLKAIHKAPSAQVVSIEESN KRGKILFNVNVSPWRSTDGKGQQPY
Sbjct: 89  LFPLLEETRAELCSSLKAIHKAPSAQVVSIEESNAKRGKILFNVNVSPWRSTDGKGQQPY 148

Query: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180
           KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHL LHVSKNITA GD
Sbjct: 149 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLNLHVSKNITAHGD 208

Query: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240
           ILKSTT FIVFLMNVTTNLRIWKALQCSAGGGII RVLGTTWLGNHQSCTECTQND+EDP
Sbjct: 209 ILKSTTLFIVFLMNVTTNLRIWKALQCSAGGGIIGRVLGTTWLGNHQSCTECTQNDQEDP 268

Query: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300
           TQDYPTPH SSLNESQKVAI+TCI+NTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM
Sbjct: 269 TQDYPTPHPSSLNESQKVAIETCIQNTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 328

Query: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360
           KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE
Sbjct: 329 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 388

Query: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420
           EIYVEHRVEKLIKCLG NGWKFQITSMIEILQG KFPRLKRMFKSIASSLLECVHILTTH
Sbjct: 389 EIYVEHRVEKLIKCLGANGWKFQITSMIEILQGCKFPRLKRMFKSIASSLLECVHILTTH 448

Query: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480
           VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGL+GQCVLVLQTLLMSLDQV
Sbjct: 449 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLRGQCVLVLQTLLMSLDQV 508

Query: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540
           EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL
Sbjct: 509 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 568

Query: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600
           QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS
Sbjct: 569 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 628

Query: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660
           HFPNSKFYGNQILDASIVMNKQLYEE+YLPSPLFGPYSFI+VSGGQEESNDDGQSKKNMF
Sbjct: 629 HFPNSKFYGNQILDASIVMNKQLYEEYYLPSPLFGPYSFINVSGGQEESNDDGQSKKNMF 688

Query: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYE-KEGFTIKVKSVD 720
           EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYE KEGFTIKVKSVD
Sbjct: 689 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKKEGFTIKVKSVD 748

Query: 721 GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 780
           GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR
Sbjct: 749 GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 808

Query: 781 EVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKKSDGV 838
           EVIKDAKSR+C FNVEED+ELGDAMK MKTWQMSDINQEIL LDNIYNS HKKKSDGV
Sbjct: 809 EVIKDAKSRKCLFNVEEDKELGDAMKTMKTWQMSDINQEILNLDNIYNSDHKKKSDGV 866

BLAST of CmaCh04G000130 vs. NCBI nr
Match: XP_022942074.1 (helicase SEN1-like [Cucurbita moschata])

HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 810/834 (97.12%), Postives = 818/834 (98.08%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCR HK +SAKDSNGLIDVLFSWEL NVFNQNLYKLKVGNIPKSFESEEHYRGSY
Sbjct: 1   MEGGESCRRHKPSSAKDSNGLIDVLFSWELTNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120
           LFPL EETRAELCSSLKAIHKAPSAQVVSIEESN KRGKILFNVNVSPWRSTDGKGQQPY
Sbjct: 61  LFPLLEETRAELCSSLKAIHKAPSAQVVSIEESNAKRGKILFNVNVSPWRSTDGKGQQPY 120

Query: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180
           KALPG IFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHL LHVSKNITAQGD
Sbjct: 121 KALPGHIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLNLHVSKNITAQGD 180

Query: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240
           IL+STT FIVFLMNVTTNLRIWKALQCSAGGGII RVLGTTWLGNHQSCTECTQND+EDP
Sbjct: 181 ILESTTLFIVFLMNVTTNLRIWKALQCSAGGGIIGRVLGTTWLGNHQSCTECTQNDQEDP 240

Query: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300
           TQDYPTPH SSLNESQKVAI+TCI+NTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM
Sbjct: 241 TQDYPTPHPSSLNESQKVAIETCIQNTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300

Query: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360
           KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE
Sbjct: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360

Query: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420
           EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQG KFPRLKRMFKSIASSLLECVHILTTH
Sbjct: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGCKFPRLKRMFKSIASSLLECVHILTTH 420

Query: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480
           VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV
Sbjct: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480

Query: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540
           EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL
Sbjct: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540

Query: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600
           QLPDIRHAILIGDEFQLPAT+SSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS
Sbjct: 541 QLPDIRHAILIGDEFQLPATISSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600

Query: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660
           HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFI+VSGGQEESNDDGQSKKNMF
Sbjct: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFINVSGGQEESNDDGQSKKNMF 660

Query: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYE-KEGFTIKVKSVD 720
           EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYE KEGFTIKVKSVD
Sbjct: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKKEGFTIKVKSVD 720

Query: 721 GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 780
           GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR
Sbjct: 721 GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 780

Query: 781 EVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKK 834
           EVIKDAKSRQC FNVEED+ELGDAMKMMKT QMSDINQEIL LDNIYNS HKKK
Sbjct: 781 EVIKDAKSRQCLFNVEEDKELGDAMKMMKTSQMSDINQEILNLDNIYNSDHKKK 834

BLAST of CmaCh04G000130 vs. NCBI nr
Match: KAG6599862.1 (hypothetical protein SDJN03_05095, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1611.7 bits (4172), Expect = 0.0e+00
Identity = 806/834 (96.64%), Postives = 818/834 (98.08%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCRSHKA+SAKDSNGLIDVLFSWEL NVFNQNLYKLKVGNIPKSFESEEHYRGSY
Sbjct: 1   MEGGESCRSHKASSAKDSNGLIDVLFSWELTNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120
           LFPL EETRAELCSSLKAIHKAPSAQVVSIEESN KRGKILFNVNVSPWRSTDGKGQQPY
Sbjct: 61  LFPLLEETRAELCSSLKAIHKAPSAQVVSIEESNAKRGKILFNVNVSPWRSTDGKGQQPY 120

Query: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180
           KALPGDIF+ILDSDPQTTDSD LERSEFNWAFAWLGQITDNSIPTHL L VSKNITAQGD
Sbjct: 121 KALPGDIFLILDSDPQTTDSDCLERSEFNWAFAWLGQITDNSIPTHLNLRVSKNITAQGD 180

Query: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240
           ILKSTT FIVFLMNVTTNLRIWKALQCSAGGGII RVLGTTWLGNHQSCTECTQND+EDP
Sbjct: 181 ILKSTTLFIVFLMNVTTNLRIWKALQCSAGGGIIGRVLGTTWLGNHQSCTECTQNDQEDP 240

Query: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300
           TQDYPTPH SSLNESQKVAI+TCI+NTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM
Sbjct: 241 TQDYPTPHPSSLNESQKVAIETCIQNTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300

Query: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360
           KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGN DRLKVDSQLE
Sbjct: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNNDRLKVDSQLE 360

Query: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420
           EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQG KFPRLKRMFKSIASSLLECVHILTTH
Sbjct: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGCKFPRLKRMFKSIASSLLECVHILTTH 420

Query: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480
           VPQ+VIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV
Sbjct: 421 VPQQVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480

Query: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540
           EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVK+NSLNLLVVDEAAQLKECESLIPL
Sbjct: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKRNSLNLLVVDEAAQLKECESLIPL 540

Query: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600
           QLPDIRHAILIGDEFQLPAT+SSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS
Sbjct: 541 QLPDIRHAILIGDEFQLPATISSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600

Query: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660
           HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFI+VSGGQEESNDDGQSKKNMF
Sbjct: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFINVSGGQEESNDDGQSKKNMF 660

Query: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYE-KEGFTIKVKSVD 720
           EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQH+LGRKYE KEGFTIKVKSVD
Sbjct: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHRLGRKYEKKEGFTIKVKSVD 720

Query: 721 GFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 780
           GFQGGEEDVIIISTVRSN GNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR
Sbjct: 721 GFQGGEEDVIIISTVRSNPGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWR 780

Query: 781 EVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLDNIYNSGHKKK 834
           EVIKDAKSRQC FNVEED+ELGDAMKMMKTWQMSDINQEIL LDNIY+S HKKK
Sbjct: 781 EVIKDAKSRQCLFNVEEDKELGDAMKMMKTWQMSDINQEILNLDNIYDSDHKKK 834

BLAST of CmaCh04G000130 vs. NCBI nr
Match: KAG7030540.1 (SEN1-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1321.2 bits (3418), Expect = 0.0e+00
Identity = 677/802 (84.41%), Postives = 687/802 (85.66%), Query Frame = 0

Query: 1   MEGGESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 60
           MEGGESCR HK +SAKDSNGLIDVLFSWEL NVFNQNLYKLKVGNIPKSFESEEHYRGSY
Sbjct: 29  MEGGESCRRHKPSSAKDSNGLIDVLFSWELTNVFNQNLYKLKVGNIPKSFESEEHYRGSY 88

Query: 61  LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDGKGQQPY 120
           LFPL EETRAELCSSLKAIHKAPSAQVVSIEESN KRGKILFNVNVSPWRSTDGKGQQPY
Sbjct: 89  LFPLLEETRAELCSSLKAIHKAPSAQVVSIEESNAKRGKILFNVNVSPWRSTDGKGQQPY 148

Query: 121 KALPGDIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD 180
           KALPG IFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHL LHVSKNITAQGD
Sbjct: 149 KALPGHIFIILDSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLNLHVSKNITAQGD 208

Query: 181 ILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDP 240
           IL+STT FIVFLMNVTTNLRIWKALQCSAGGGII RVLGTTWLGNHQSCTECTQND+EDP
Sbjct: 209 ILESTTLFIVFLMNVTTNLRIWKALQCSAGGGIIGRVLGTTWLGNHQSCTECTQNDQEDP 268

Query: 241 TQDYPTPHLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 300
           TQDYPTPH SSLNESQKVAI+TCI+NTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM
Sbjct: 269 TQDYPTPHPSSLNESQKVAIETCIQNTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTM 328

Query: 301 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 360
           KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE
Sbjct: 329 KHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQLE 388

Query: 361 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLECVHILTTH 420
           EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQG KFPRLKRMFKSIASSLLECVHILTTH
Sbjct: 389 EIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGCKFPRLKRMFKSIASSLLECVHILTTH 448

Query: 421 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 480
           VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV
Sbjct: 449 VPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDKVRGTLIGLKGQCVLVLQTLLMSLDQV 508

Query: 481 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 540
           EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL
Sbjct: 509 EVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPL 568

Query: 541 QLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 600
           QLPDIRHAILIGDEFQLPAT+SSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS
Sbjct: 569 QLPDIRHAILIGDEFQLPATISSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVS 628

Query: 601 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMF 660
           HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFI+VSGGQEESNDDGQSKKNMF
Sbjct: 629 HFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFINVSGGQEESNDDGQSKKNMF 688

Query: 661 EVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEGFTIKVKSVDG 720
           E +                                                         
Sbjct: 689 EAI--------------------------------------------------------- 733

Query: 721 FQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWRE 780
                                                   CLWIVGDAT LGKSNSEWRE
Sbjct: 749 ----------------------------------------CLWIVGDATNLGKSNSEWRE 733

Query: 781 VIKDAKSRQCF-FNVEEDEELG 802
           VIKDAKSRQ    NVEED+ +G
Sbjct: 809 VIKDAKSRQMLCLNVEEDKRVG 733

BLAST of CmaCh04G000130 vs. TAIR 10
Match: AT1G65810.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 553.9 bits (1426), Expect = 2.2e-157
Identity = 349/847 (41.20%), Postives = 497/847 (58.68%), Query Frame = 0

Query: 21  LIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSYLFPLFEETRAELCSSLKAIH 80
           L+DV+FSW LR+V N NLY+ +VG IP +F S + Y  S++ P+ EET A+L SS+  I 
Sbjct: 22  LVDVVFSWSLRDVLNSNLYRGQVGKIPNTFTSTKEYFESFVKPIIEETHADLLSSMGTIR 81

Query: 81  KAPSAQVVSIEESNTKR--GKILFNVNVSPWRSTDGKGQQPYKALPGDIFIILDSDPQTT 140
           +A + +   I+     +    + + V +        KG Q    +  D+  + D  P   
Sbjct: 82  RAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEV-NDLIAVTDKRPIRI 141

Query: 141 DSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD------------ILKSTT 200
           D   L  S   +  A +  + +N+ P  + +  SK I    D              KS +
Sbjct: 142 DD--LRFSHEPYLLALVCGVNENN-PHLITILASKPIIFDDDDDIKTSSKRGKGERKSLS 201

Query: 201 FFIVFLMNVTTNLRIWKALQCSAGGG---IIERVLGTTWLGNHQSCTECTQNDKEDPTQD 260
           FF V L+N+ TN+RIW AL  +  GG   +I RVL +    +  SC  C +N  E    D
Sbjct: 202 FFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSCKEN-SESVVSD 261

Query: 261 YPTPHLSS--LNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMK 320
           Y    L S  LN SQ+ AI  C+E   C H  +I LIWGPPGTGKTKTTS+LL   L M+
Sbjct: 262 YSARMLRSFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTSVLLLNFLKMR 321

Query: 321 HQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQ--L 380
              RTL C+PTN+A+  + S++VKL+  ES R D     LG ++LFGNK+R+K+D +  L
Sbjct: 322 --CRTLTCAPTNIAVLEVCSRLVKLV-SESLRFDGY--GLGDIVLFGNKERMKIDDREDL 381

Query: 381 EEIYVEHRVEKLIKC-LGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLL------- 440
            ++++E+RV++L +C +   GW+  +  MI +L   K     R FKS+ ++LL       
Sbjct: 382 FDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPKHE--FRQFKSVNTTLLSFKDFVE 441

Query: 441 --------ECVHILTT---HVPQEVIMEHNLKKMEILVELIGDIGT--LLSEDDDKVRGT 500
                   +  H  TT   H+P  ++     +KM     L+ +I    ++ +   +++  
Sbjct: 442 ERLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNIAASDVMRDGYGRMKYK 501

Query: 501 LIGLKGQ-------CVLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFK 560
           L     +       C+ +L ++ MS   +++P  +S+  ++K C   A L+F TAS+S +
Sbjct: 502 LKDTGDENDSRTQDCLEMLTSISMS---IKLPDFISKFELQKLCLDNAYLLFCTASSSAR 561

Query: 561 LKNVKKNSLNLLVVDEAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGF 620
           L     + + LLV+DEAAQLKECES IPLQL  ++HAILIGDE QLPA + S ++  A  
Sbjct: 562 LH--MSSPIQLLVIDEAAQLKECESAIPLQLRGLQHAILIGDEKQLPAMIKSNIASEADL 621

Query: 621 GTSLFERLSVLGHFKHLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPS 680
           G SLFERL +LGH K LLN QYRMHPS+S FPN +FY  +ILDA  V  +  YE+ +LP 
Sbjct: 622 GRSLFERLVLLGHNKQLLNMQYRMHPSISIFPNREFYDMKILDAPSVRLRS-YEKKFLPE 681

Query: 681 PLFGPYSFIDVSGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPY 740
            ++GPYSFI+++ G+E+   +G S KN+ EV VVA+I+  LY       + IS+GVISPY
Sbjct: 682 KMYGPYSFINIAYGREQFG-EGYSSKNLVEVSVVAEIVSKLYSVSRKTGRTISVGVISPY 741

Query: 741 AAQVSSIQHKLGRKYEKEG-FTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQR 800
            AQV +IQ ++G KY  EG FT+ V+SVDGFQGGEED+IIISTVRSN    IGFLS+ QR
Sbjct: 742 KAQVFAIQERIGEKYNTEGTFTVSVRSVDGFQGGEEDIIIISTVRSNGNGAIGFLSNQQR 801

Query: 801 TNVALTRARHCLWIVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTW 818
           TNVALTRAR+CLWI+G+  TL  + S WR+++ DAK+R CF N EEDE L   ++   T 
Sbjct: 802 TNVALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARNCFHNAEEDESLAQCIE-RSTT 848

BLAST of CmaCh04G000130 vs. TAIR 10
Match: AT1G65810.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 553.9 bits (1426), Expect = 2.2e-157
Identity = 349/847 (41.20%), Postives = 497/847 (58.68%), Query Frame = 0

Query: 21  LIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSYLFPLFEETRAELCSSLKAIH 80
           L+DV+FSW LR+V N NLY+ +VG IP +F S + Y  S++ P+ EET A+L SS+  I 
Sbjct: 22  LVDVVFSWSLRDVLNSNLYRGQVGKIPNTFTSTKEYFESFVKPIIEETHADLLSSMGTIR 81

Query: 81  KAPSAQVVSIEESNTKR--GKILFNVNVSPWRSTDGKGQQPYKALPGDIFIILDSDPQTT 140
           +A + +   I+     +    + + V +        KG Q    +  D+  + D  P   
Sbjct: 82  RAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEV-NDLIAVTDKRPIRI 141

Query: 141 DSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGD------------ILKSTT 200
           D   L  S   +  A +  + +N+ P  + +  SK I    D              KS +
Sbjct: 142 DD--LRFSHEPYLLALVCGVNENN-PHLITILASKPIIFDDDDDIKTSSKRGKGERKSLS 201

Query: 201 FFIVFLMNVTTNLRIWKALQCSAGGG---IIERVLGTTWLGNHQSCTECTQNDKEDPTQD 260
           FF V L+N+ TN+RIW AL  +  GG   +I RVL +    +  SC  C +N  E    D
Sbjct: 202 FFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSCKEN-SESVVSD 261

Query: 261 YPTPHLSS--LNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMK 320
           Y    L S  LN SQ+ AI  C+E   C H  +I LIWGPPGTGKTKTTS+LL   L M+
Sbjct: 262 YSARMLRSFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTSVLLLNFLKMR 321

Query: 321 HQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQ--L 380
              RTL C+PTN+A+  + S++VKL+  ES R D     LG ++LFGNK+R+K+D +  L
Sbjct: 322 --CRTLTCAPTNIAVLEVCSRLVKLV-SESLRFDGY--GLGDIVLFGNKERMKIDDREDL 381

Query: 381 EEIYVEHRVEKLIKC-LGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLL------- 440
            ++++E+RV++L +C +   GW+  +  MI +L   K     R FKS+ ++LL       
Sbjct: 382 FDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPKHE--FRQFKSVNTTLLSFKDFVE 441

Query: 441 --------ECVHILTT---HVPQEVIMEHNLKKMEILVELIGDIGT--LLSEDDDKVRGT 500
                   +  H  TT   H+P  ++     +KM     L+ +I    ++ +   +++  
Sbjct: 442 ERLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNIAASDVMRDGYGRMKYK 501

Query: 501 LIGLKGQ-------CVLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFK 560
           L     +       C+ +L ++ MS   +++P  +S+  ++K C   A L+F TAS+S +
Sbjct: 502 LKDTGDENDSRTQDCLEMLTSISMS---IKLPDFISKFELQKLCLDNAYLLFCTASSSAR 561

Query: 561 LKNVKKNSLNLLVVDEAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGF 620
           L     + + LLV+DEAAQLKECES IPLQL  ++HAILIGDE QLPA + S ++  A  
Sbjct: 562 LH--MSSPIQLLVIDEAAQLKECESAIPLQLRGLQHAILIGDEKQLPAMIKSNIASEADL 621

Query: 621 GTSLFERLSVLGHFKHLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPS 680
           G SLFERL +LGH K LLN QYRMHPS+S FPN +FY  +ILDA  V  +  YE+ +LP 
Sbjct: 622 GRSLFERLVLLGHNKQLLNMQYRMHPSISIFPNREFYDMKILDAPSVRLRS-YEKKFLPE 681

Query: 681 PLFGPYSFIDVSGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPY 740
            ++GPYSFI+++ G+E+   +G S KN+ EV VVA+I+  LY       + IS+GVISPY
Sbjct: 682 KMYGPYSFINIAYGREQFG-EGYSSKNLVEVSVVAEIVSKLYSVSRKTGRTISVGVISPY 741

Query: 741 AAQVSSIQHKLGRKYEKEG-FTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQR 800
            AQV +IQ ++G KY  EG FT+ V+SVDGFQGGEED+IIISTVRSN    IGFLS+ QR
Sbjct: 742 KAQVFAIQERIGEKYNTEGTFTVSVRSVDGFQGGEEDIIIISTVRSNGNGAIGFLSNQQR 801

Query: 801 TNVALTRARHCLWIVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTW 818
           TNVALTRAR+CLWI+G+  TL  + S WR+++ DAK+R CF N EEDE L   ++   T 
Sbjct: 802 TNVALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARNCFHNAEEDESLAQCIE-RSTT 848

BLAST of CmaCh04G000130 vs. TAIR 10
Match: AT5G37150.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 508.4 bits (1308), Expect = 1.1e-143
Identity = 313/838 (37.35%), Postives = 472/838 (56.32%), Query Frame = 0

Query: 17  DSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSYLFPLFEETRAELCSSL 76
           +   L+D +FSW ++++ N++ YK K   +P  F S + Y   ++  L  E   EL SSL
Sbjct: 5   EKTSLVDRVFSWSIKDILNKDFYKQK--TVPDKFRSVDEYYQCFVPHLLIEAHTELFSSL 64

Query: 77  KAIHKAPSAQVVSIE-----ESNTKRGKILFNVNVSPWRSTDGKGQQPYKALPGDIFIIL 136
           K++ K+P  Q+ S+E      S +   K+ +++ +    S   K    Y+   GD+  + 
Sbjct: 65  KSVSKSPFVQIRSMETKTKQSSGSSSNKLFYDITLKATESLSAK----YQPKCGDLIALT 124

Query: 137 DSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGDILKSTTFFIVF 196
              P+  +   L      + F+  G +        + +H+S++I+     L++ +F  VF
Sbjct: 125 MDKPRRIND--LNPLLLAYVFSSDGDL-------KISVHLSRSISP----LENYSFG-VF 184

Query: 197 LMNVTTNLRIWKALQCSAG-GGIIERVLGTTWLGNHQSCTECTQNDKEDPTQDYPTPHLS 256
           LM +TTN RIW AL   A    + + VL    + N             D T        +
Sbjct: 185 LMTLTTNTRIWNALHNEAAISTLTKSVLQANTVNN-----VFVLKMMGDLTLFLDIIRST 244

Query: 257 SLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMKHQIRTLACS 316
            LN SQ+ AI  C+E   C HK S+ LIWGPPGTGKTKT + LL+ +L  K + +T+ C+
Sbjct: 245 KLNSSQEDAILGCLETRNCTHKNSVKLIWGPPGTGKTKTVATLLFALL--KLRCKTVVCA 304

Query: 317 PTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRL---KVDSQLEEIYVEHR 376
           PTN AI  +AS+++ L K+ S  ++  +  LG ++L GN+DR+   K D  L +++++ R
Sbjct: 305 PTNTAIVQVASRLLSLFKENSTSENATY-RLGNIILSGNRDRMGIHKNDHVLLDVFLDER 364

Query: 377 VEKLIKCLGP-NGWKFQITSMIEILQG--SKFPR-------------------------- 436
           + KL K   P +GW  ++ S+I+ L+    K+ R                          
Sbjct: 365 IGKLGKLFSPFSGWMQRLESLIQFLENPEGKYERHVYELEEVERMEEEAERQEVVVNIPT 424

Query: 437 ----LKRMFKSIASSLLECVHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSEDDDK 496
               +K+ F S++  +  C+  L TH+P+  +   ++K M    + +  I   L E+  +
Sbjct: 425 IGEFVKKNFNSLSEEVETCIVDLFTHLPKVYLPYDDVKIMIASRQSLQRIRYFLRENSSR 484

Query: 497 VRGTLIGLKGQC-----VLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNS 556
           V       +  C     V  L+ L +   + E+P  +    I KFC Q A +I  TAS +
Sbjct: 485 VDFEEGNFRFDCFKRLSVDCLKALRLLPKRFEIPDMLENEDIRKFCLQNADIILCTASGA 544

Query: 557 FKLKNVKKNSLNLLVVDEAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAA 616
            ++   +  ++ LLVVDEAAQLKECES+  LQLP +RHAILIGDEFQLPA V +++ E A
Sbjct: 545 AEMNVERTGNVELLVVDEAAQLKECESVAALQLPGLRHAILIGDEFQLPAMVHNEMCEKA 604

Query: 617 GFGTSLFERLSVLGHFKHLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYL 676
            FG SLFERL +LGH KHLL+ QYRMHPS+S FPN +FYG +I DA  V  + +Y++ +L
Sbjct: 605 KFGRSLFERLVLLGHNKHLLDVQYRMHPSISRFPNKEFYGGRIKDAENV-KESIYQKRFL 664

Query: 677 PSPLFGPYSFIDVSGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVIS 736
              +FG +SFI+V  G+EE   DG S KNM EV VV++II  L+K    ++  +S+GV+S
Sbjct: 665 QGNMFGSFSFINVGRGKEEFG-DGHSPKNMVEVAVVSEIISNLFKVSCERRMKVSVGVVS 724

Query: 737 PYAAQVSSIQHKLGRKYEK---EGFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLS 796
           PY  Q+ +IQ K+G KY     + F + V+SVDGFQGGEED+IIISTVRSN    +GFL+
Sbjct: 725 PYKGQMRAIQEKIGDKYSSLSGQQFALNVRSVDGFQGGEEDIIIISTVRSNSNGKVGFLN 784

Query: 797 SSQRTNVALTRARHCLWIVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAM 805
           + QR NVALTRARHCLW++G+ TTL  S S W  +I ++++R CF++  ++  L +AM
Sbjct: 785 NRQRANVALTRARHCLWVIGNETTLALSGSIWATLISESRTRGCFYDATDEMNLRNAM 812

BLAST of CmaCh04G000130 vs. TAIR 10
Match: AT5G37160.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 492.3 bits (1266), Expect = 7.9e-139
Identity = 318/850 (37.41%), Postives = 472/850 (55.53%), Query Frame = 0

Query: 17  DSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSYLFPLFEETRAELCSSL 76
           +   L   L SW L+++ N++L K K+  IP  F S + Y   ++  L EETR EL SS 
Sbjct: 14  EQTSLFARLCSWSLKDILNEDLSKEKIMTIPDRFSSVDEYSQCFVPHLLEETRTELFSSF 73

Query: 77  KAIHKAPSAQVVSIEE---SNTKRGKILFNVNVSPWRSTDGKGQQPYKALPGDIFII--L 136
           +++ K+P ++++S+E      + R  I +  ++      D K  + Y+   GDI  +  L
Sbjct: 74  RSLSKSPVSRILSVETKVIEYSGRSSIKWFHDIKLMDYADDK-NEIYEPKCGDIIALSPL 133

Query: 137 DSDPQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGDILKSTTFFIVF 196
               +    D L+     + F+  G   D+ I  H    +S++        K T    VF
Sbjct: 134 SLTEERPRIDDLDPLLLGYVFSVYG---DSKISVHFSRSISQS-------EKHTFCTGVF 193

Query: 197 LMNVTTNLRIWKAL-QCSAGGGIIERVLGTTWLGNHQSCTECTQNDKEDPTQDYPTPHLS 256
           L+N+TTN RIW AL + +A   +I+ VL        Q C  C +ND +    D     + 
Sbjct: 194 LINITTNTRIWNALHKDAADSTLIQSVLQEDASATEQ-CFSC-ENDVDGSDSDRVVDIIR 253

Query: 257 S--LNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMKHQIRTLA 316
           S  LN SQ+ AI   ++   C+HK S+ LIWGPPGTGKTKT + LL  ++ +K   +T+ 
Sbjct: 254 SAKLNSSQEAAILGFLKTRNCKHKESVKLIWGPPGTGKTKTVATLLSTLMQLK--CKTVV 313

Query: 317 CSPTNVAITNLASQVVKLLKD---------------ESFRKDHIF----------CPLGQ 376
           C+PTN  I  +AS+++ L K+                 F    +F            +G 
Sbjct: 314 CAPTNTTIVAVASRLLSLSKETIVCAPTNSAIAEVVSRFEFSTLFYGTSILERTTYGMGN 373

Query: 377 LLLFGNKDRLKVDSQ--LEEIYVEHRVEKLIKC-LGPNGWKFQITSMIEILQGSKFP--- 436
           ++L GN++R+ + S   L  ++   RV KL +  L   GWK ++ S+I+ L+ ++     
Sbjct: 374 IVLSGNRERMGITSNKVLLNVFFNDRVSKLGRLFLSTCGWKKRLESIIDFLENTETKYEQ 433

Query: 437 -----RLKRMF------KSIASSLLECVHI--LTTHVPQEVIMEHNLKKMEILVELIGDI 496
                 L+RM       + +    ++ V +  L+TH+P+  I   ++K +    + +  +
Sbjct: 434 HVNELELERMTEDEKKKEEVEERTMQEVDMADLSTHLPKSFISSKDVKNLIAACQALHRV 493

Query: 497 GTLLSEDDDKVRGTLIGLKGQC------VLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQ 556
              L E+  +      G +  C      V  LQ L +      +    +   I KFC Q 
Sbjct: 494 RYFLQENSSRDDFKKGGFRFNCFNKLISVDALQALCLLPKCFGIFGLANNEDIRKFCLQN 553

Query: 557 ASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPLQLPDIRHAILIGDEFQLP 616
           A +IF TAS+   +   +  S++LLVVDE AQLKECES+  LQLP + HA+LIGDE+QLP
Sbjct: 554 ADIIFCTASSVANINPARIGSVDLLVVDETAQLKECESVAALQLPGLCHALLIGDEYQLP 613

Query: 617 ATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVSHFPNSKFYGNQILDASIV 676
           A V ++  + A FG SLFERL ++GH KHLLN QYRMHPS+S FPN +FYG +I DA+ V
Sbjct: 614 AMVHNEECDKAKFGRSLFERLVLIGHSKHLLNVQYRMHPSISRFPNKEFYGGRITDAANV 673

Query: 677 MNKQLYEEHYLPSPLFGPYSFIDVSGGQEESNDDGQSKKNMFEVVVVAQIIQMLYKAWSN 736
             + +YE+ +L   +FG +SFI+V  G+EE   DG S KNM EV V+++II  L+K  S 
Sbjct: 674 -QESIYEKRFLQGNMFGTFSFINVGRGKEEFG-DGHSPKNMVEVAVISKIISNLFKVSSQ 733

Query: 737 KKKDISIGVISPYAAQVSSIQHKLGRKYEK----EGFTIKVKSVDGFQGGEEDVIIISTV 796
           +K+ +S+GVISPY  QV +IQ ++G KY      + FT+ V+SVDGFQGGE DVIIISTV
Sbjct: 734 RKQKMSVGVISPYKGQVRAIQERVGDKYNSLSVDQLFTLNVQSVDGFQGGEVDVIIISTV 793

Query: 797 RSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEWREVIKDAKSRQCFFNV 805
           R N   N+GFLS+ QR NVALTRARHCLW++G+ TTL  S S W E+I ++++R CF++ 
Sbjct: 794 RCNVNGNVGFLSNRQRANVALTRARHCLWVIGNGTTLALSGSIWAELISESRTRGCFYDA 846

BLAST of CmaCh04G000130 vs. TAIR 10
Match: AT1G65780.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 486.9 bits (1252), Expect = 3.3e-137
Identity = 323/866 (37.30%), Postives = 461/866 (53.23%), Query Frame = 0

Query: 17  DSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSYLFPLFEETRAELCSSL 76
           +   L+D++ SW L  V N +LYK +V  IP  FES   Y  +++ PL EET A L SS+
Sbjct: 7   EGRDLVDLVLSWSLDEVLNVDLYKGQVEKIPMEFESTGDYFKTFIPPLIEETHAALLSSM 66

Query: 77  KAIHKAPSAQVVSIEESNTKR--GKILFNVNVSPWRSTDGKGQQPYKALPGDIFIILDSD 136
           + + +AP  ++  I ++   +    + + V +S         +   K +P D+  + D  
Sbjct: 67  RKLWQAPVVEISYIMQTAEYKLPNDLFYKVRLS-----GISNEASTKLMPRDLISLTDQR 126

Query: 137 PQTTDSDYLERSEFNWAFAWLGQITDNSIPTHLKLHVSKNITAQGDILKST----TFFIV 196
           P   D   +    +  A        D   P  + +  SK +  +    K        F +
Sbjct: 127 PNHVDGFNISSEPYIVALVCK---VDPDRPNDVTILASKPLFVEDGRRKKNEKKERLFGI 186

Query: 197 FLMNVTTNLRIWKALQCSAGG---GIIERVLGTTWLGNHQSCTECTQNDKEDPTQDYPTP 256
            L+N+TTN+RIW AL     G    +I RVL      +   C +C Q    + +      
Sbjct: 187 HLVNLTTNIRIWNALHPGDEGVNLNLISRVLRRN-SEDEGFCIQCLQ----EGSDGLAPR 246

Query: 257 HLSSLNESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLWRILTMKHQIRTL 316
               LN SQ+ AI  C++   C H  ++ LIWGPPGTGKTKTTS+LL+ +L  K   RTL
Sbjct: 247 RFLKLNPSQEDAILNCLDVRRCYHANTVRLIWGPPGTGKTKTTSVLLFTLLNAK--CRTL 306

Query: 317 ACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKVDSQ--LEEIYVE 376
            C PTNV++  +AS+V+KL+       ++    LG ++LFGN +R+K+  +  L  I+++
Sbjct: 307 TCGPTNVSVLEVASRVLKLVSGSLKIGNY---GLGDVVLFGNDERMKIKDRKDLVNIFID 366

Query: 377 HRVEKLIKCLGP-NGWKFQITSMIEILQ------------------------GSKFPR-- 436
            RV+KL  C  P  GWK  I  MI +L+                        GS F R  
Sbjct: 367 ERVDKLYPCFMPFYGWKATIDGMIRLLEDPKGQYNLYLENLARANNVKRKDTGSVFKRKG 426

Query: 437 ----------------------LKRMFKSIASSLLECVHILTTHVPQEVIMEHNLKKMEI 496
                                 L   F  +   L      L TH+P  ++      +M  
Sbjct: 427 NEQNENIVEQVSDTRPQSFQDYLPEKFSELRKDLDLHFSSLCTHLPTALLSSQAATRMYE 486

Query: 497 LVELIGDIGTLLSED---DDKVRGTLI-------GLKGQCVLV---LQTLLMSLDQV-EV 556
            ++L+ D+  L   D    + V+  LI           Q V V      LL S+ ++  +
Sbjct: 487 AIDLVRDVTILAILDGVTGEGVKSVLIPNGEGSDRFSSQHVTVEDDYLKLLRSIPEIFPL 546

Query: 557 PSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVDEAAQLKECESLIPLQL 616
           P+   R+ I++ C   A L+FSTAS S +L       + LLV+DEAAQLKECES IP+QL
Sbjct: 547 PAVSDRHLIKELCLGHACLLFSTASCSARL--YTGTPIQLLVIDEAAQLKECESSIPMQL 606

Query: 617 PDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFKHLLNTQYRMHPSVSHF 676
           P +RH IL+GDE QLPA V S+++  AGFG SLFERL++LGH K++LN QYRMH S+S F
Sbjct: 607 PGLRHLILVGDERQLPAMVESQIALEAGFGRSLFERLALLGHKKYMLNIQYRMHCSISSF 666

Query: 677 PNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQEESND-DGQSKKNMFE 736
           PN + YG +ILDA  V  +  Y + YLP  ++GPYSFI+++ G+EE  + +G+S KN  E
Sbjct: 667 PNKELYGKKILDAPTVRQRN-YTKQYLPGEMYGPYSFINIAYGREEYGEGEGRSLKNNVE 726

Query: 737 VVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKYEKEG---FTIKVKSV 796
           VVVVA II  L +     K  I++GVISPY AQV +IQ K+      +    F++++++V
Sbjct: 727 VVVVAAIIANLLQVSEKTKTRINVGVISPYKAQVIAIQEKIQETSIGDAGGLFSLRIRTV 786

Query: 797 DGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLWIVGDATTLGKSNSEW 805
           DGFQGGEED+II+STVRSN    +GFL + +RTNV LTRAR CLWI+G+  TL  S S W
Sbjct: 787 DGFQGGEEDIIIVSTVRSNGVGRVGFLGNRRRTNVLLTRARFCLWILGNEATLMNSKSVW 846

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q004161.8e-4728.55Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292... [more]
B6SFA41.2e-4635.82Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1[more]
Q923559.9e-4628.20Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 G... [more]
A2AKX33.2e-4427.79Probable helicase senataxin OS=Mus musculus OX=10090 GN=Setx PE=1 SV=1[more]
O943871.8e-4228.59Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (... [more]
Match NameE-valueIdentityDescription
A0A6J1JC410.0e+00100.00helicase sen1-like OS=Cucurbita maxima OX=3661 GN=LOC111485435 PE=4 SV=1[more]
A0A6J1FQ960.0e+0097.12helicase SEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111447261 PE=4 SV=1[more]
A0A1S3C4A00.0e+0072.54helicase SEN1-like OS=Cucumis melo OX=3656 GN=LOC103496680 PE=4 SV=1[more]
A0A0A0KMQ30.0e+0073.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G292250 PE=4 SV=1[more]
A0A6J1DR270.0e+0073.28helicase SEN1-like OS=Momordica charantia OX=3673 GN=LOC111023039 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022988087.10.0e+00100.00helicase sen1-like [Cucurbita maxima][more]
XP_023517650.10.0e+0097.14helicase SEN1-like [Cucurbita pepo subsp. pepo][more]
XP_022942074.10.0e+0097.12helicase SEN1-like [Cucurbita moschata][more]
KAG6599862.10.0e+0096.64hypothetical protein SDJN03_05095, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7030540.10.0e+0084.41SEN1-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G65810.12.2e-15741.20P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G65810.22.2e-15741.20P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G37150.11.1e-14337.35P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G37160.17.9e-13937.41P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G65780.13.3e-13737.30P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 197..592
e-value: 5.8E-48
score: 165.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 593..806
e-value: 1.2E-59
score: 203.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 245..785
IPR041677DNA2/NAM7 helicase, helicase domainPFAMPF13086AAA_11coord: 252..564
e-value: 3.7E-31
score: 108.9
IPR041679DNA2/NAM7 helicase-like, C-terminalPFAMPF13087AAA_12coord: 572..768
e-value: 2.3E-60
score: 203.7
IPR041679DNA2/NAM7 helicase-like, C-terminalCDDcd18808SF1_C_Upf1coord: 595..786
e-value: 3.47538E-59
score: 197.457
NoneNo IPR availablePANTHERPTHR10887:SF474OS04G0582700 PROTEINcoord: 14..807
IPR045055DNA2/NAM7-like helicasePANTHERPTHR10887DNA2/NAM7 HELICASE FAMILYcoord: 14..807

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G000130.1CmaCh04G000130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
molecular_function GO:0003993 acid phosphatase activity
molecular_function GO:0004386 helicase activity
molecular_function GO:0052745 inositol phosphate phosphatase activity