CmaCh03G014040 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G014040
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description9-cis-epoxycarotenoid dioxygenase
LocationCma_Chr03: 8847545 .. 8849311 (+)
RNA-Seq ExpressionCmaCh03G014040
SyntenyCmaCh03G014040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTTCTTCTCCTTCTCCTCCTTGTCTGGATTACTTGGGGTTCCACAATTCCACGAAATCAATTAATGGTTTCAAGAATGTTAACCACATCAGGCAACCTTCCAATCACAACGCCGTTATTCATTCCGCTCTTCACTCCCCTTCTGTTCTTCATTTCCCCAATCATTCTCCTAATTTTCACGACAATCTCATTAAATCCCCTAATTCCAACTGGAATTTCCTTCAGAAATCCGCTTCAATCGCCCTCGACATGGCGGAATCCGCCTTGCTTTCCCACGAGCTCCGCCACCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCCGGCAACTTCGCTCCCGTCCCTGAACAACCCGTCAAATATAACCTCCCTGTCACCGGAACCATCCCGGATTGCATTAACGGTGTCTACCTCCGCAACGGTGCTAATCCTCTATTTGAACCCACCGCCGGTCATCACCTATTCGACGGCGACGGTATGGTCCACGCCGTCACCATCAATGACGGCTCCGCTAGCTATGCCTGCCGGTTCACGCAGACACAACGTCTGGCTCAGGAACAACAGCTCGGGCGACCGATTTTCCCTAAAGCGATTGGCGAACTCCATGGGCACTCCGGAATTGCTCGGCTGTTACTATTCTACGCGCGAGGGATGTTCGGGCTGGTGGACCATAAGAAGGGAACCGGAGTGGCGAACGCCGGTTTGGTGTATTTTAACGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGAGACCTTGAAACTGTAGGCCGTTACGATTTCAACACACAGCTTGATTCCACAATGATCGCACACCCGAAAGTGGACCCAGTCTCCAGAGAGCTCTACGCCTTAAGCTACGACGTCGTTCGGAAGCCGTATCTCAAGTACTTCCGATTCTCCCCCGACGGAATCAAATCCAAAGACGTTGAAATCCCTCTTCAAACTCCGACCATGATGCACGATTTCGCCATCACCGAGAATTTCGTTGTAATCCCCGACCAGCAAGTCGTGTTCAAGCTTCAAGAAATGCTCAAGGGCGGATCTCCGGTGATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCCAAAAATGCCACAAATTCCGATGATTTAATCTGGGTGGAATCGCCGGACACCTTCTGCTTCCATTTGTGGAATGCTTGGGAGGAGCCGGAGACAGAGGAGGTCGTGGTTATTGGGTCTTGCATGACGCCGCCGGATTCCATTTTCAACGAGTGCGATGAGAATTTGAAAAGCGTACTGTCGGAAATTCGGCTGAATTTGAGAACGGGGAAATCCACGCGCCGCCCAATTATCAGCGATGAGAAGGAGCAGGTGAATCTAGAAGCCGGAATGGTGAATAGAAACCGTCTGGGGCGGAAAACCCGATATGCGTATCTTGCGATTGCAGAGCCATGGCCGAAGGTTTCGGGATTTGCGAAGGTTGATCTAGTAACAGGGGAGGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGAGGAGAGCCGTTTTTCTTACAGAAAGAGGGGAATTCAGGGGAGAAGGAAGACGAAGGGTACATTTTAGCTTTCGTCCACGATGAAAGGACATGGAAATCAGAGTTACAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAGTGAAGCTGCCATCCAGAGTACCCTATGGCTTTCACGGGACATTCATCGACGCAAAACACTTGCAAAATCAAGCTTAA

mRNA sequence

ATGGCGGCTTCTTCTCCTTCTCCTCCTTGTCTGGATTACTTGGGGTTCCACAATTCCACGAAATCAATTAATGGTTTCAAGAATGTTAACCACATCAGGCAACCTTCCAATCACAACGCCGTTATTCATTCCGCTCTTCACTCCCCTTCTGTTCTTCATTTCCCCAATCATTCTCCTAATTTTCACGACAATCTCATTAAATCCCCTAATTCCAACTGGAATTTCCTTCAGAAATCCGCTTCAATCGCCCTCGACATGGCGGAATCCGCCTTGCTTTCCCACGAGCTCCGCCACCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCCGGCAACTTCGCTCCCGTCCCTGAACAACCCGTCAAATATAACCTCCCTGTCACCGGAACCATCCCGGATTGCATTAACGGTGTCTACCTCCGCAACGGTGCTAATCCTCTATTTGAACCCACCGCCGGTCATCACCTATTCGACGGCGACGGTATGGTCCACGCCGTCACCATCAATGACGGCTCCGCTAGCTATGCCTGCCGGTTCACGCAGACACAACGTCTGGCTCAGGAACAACAGCTCGGGCGACCGATTTTCCCTAAAGCGATTGGCGAACTCCATGGGCACTCCGGAATTGCTCGGCTGTTACTATTCTACGCGCGAGGGATGTTCGGGCTGGTGGACCATAAGAAGGGAACCGGAGTGGCGAACGCCGGTTTGGTGTATTTTAACGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGAGACCTTGAAACTGTAGGCCGTTACGATTTCAACACACAGCTTGATTCCACAATGATCGCACACCCGAAAGTGGACCCAGTCTCCAGAGAGCTCTACGCCTTAAGCTACGACGTCGTTCGGAAGCCGTATCTCAAGTACTTCCGATTCTCCCCCGACGGAATCAAATCCAAAGACGTTGAAATCCCTCTTCAAACTCCGACCATGATGCACGATTTCGCCATCACCGAGAATTTCGTTGTAATCCCCGACCAGCAAGTCGTGTTCAAGCTTCAAGAAATGCTCAAGGGCGGATCTCCGGTGATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCCAAAAATGCCACAAATTCCGATGATTTAATCTGGGTGGAATCGCCGGACACCTTCTGCTTCCATTTGTGGAATGCTTGGGAGGAGCCGGAGACAGAGGAGGTCGTGGTTATTGGGTCTTGCATGACGCCGCCGGATTCCATTTTCAACGAGTGCGATGAGAATTTGAAAAGCGTACTGTCGGAAATTCGGCTGAATTTGAGAACGGGGAAATCCACGCGCCGCCCAATTATCAGCGATGAGAAGGAGCAGGTGAATCTAGAAGCCGGAATGGTGAATAGAAACCGTCTGGGGCGGAAAACCCGATATGCGTATCTTGCGATTGCAGAGCCATGGCCGAAGGTTTCGGGATTTGCGAAGGTTGATCTAGTAACAGGGGAGGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGAGGAGAGCCGTTTTTCTTACAGAAAGAGGGGAATTCAGGGGAGAAGGAAGACGAAGGGTACATTTTAGCTTTCGTCCACGATGAAAGGACATGGAAATCAGAGTTACAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAGTGAAGCTGCCATCCAGAGTACCCTATGGCTTTCACGGGACATTCATCGACGCAAAACACTTGCAAAATCAAGCTTAA

Coding sequence (CDS)

ATGGCGGCTTCTTCTCCTTCTCCTCCTTGTCTGGATTACTTGGGGTTCCACAATTCCACGAAATCAATTAATGGTTTCAAGAATGTTAACCACATCAGGCAACCTTCCAATCACAACGCCGTTATTCATTCCGCTCTTCACTCCCCTTCTGTTCTTCATTTCCCCAATCATTCTCCTAATTTTCACGACAATCTCATTAAATCCCCTAATTCCAACTGGAATTTCCTTCAGAAATCCGCTTCAATCGCCCTCGACATGGCGGAATCCGCCTTGCTTTCCCACGAGCTCCGCCACCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCCGGCAACTTCGCTCCCGTCCCTGAACAACCCGTCAAATATAACCTCCCTGTCACCGGAACCATCCCGGATTGCATTAACGGTGTCTACCTCCGCAACGGTGCTAATCCTCTATTTGAACCCACCGCCGGTCATCACCTATTCGACGGCGACGGTATGGTCCACGCCGTCACCATCAATGACGGCTCCGCTAGCTATGCCTGCCGGTTCACGCAGACACAACGTCTGGCTCAGGAACAACAGCTCGGGCGACCGATTTTCCCTAAAGCGATTGGCGAACTCCATGGGCACTCCGGAATTGCTCGGCTGTTACTATTCTACGCGCGAGGGATGTTCGGGCTGGTGGACCATAAGAAGGGAACCGGAGTGGCGAACGCCGGTTTGGTGTATTTTAACGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGAGACCTTGAAACTGTAGGCCGTTACGATTTCAACACACAGCTTGATTCCACAATGATCGCACACCCGAAAGTGGACCCAGTCTCCAGAGAGCTCTACGCCTTAAGCTACGACGTCGTTCGGAAGCCGTATCTCAAGTACTTCCGATTCTCCCCCGACGGAATCAAATCCAAAGACGTTGAAATCCCTCTTCAAACTCCGACCATGATGCACGATTTCGCCATCACCGAGAATTTCGTTGTAATCCCCGACCAGCAAGTCGTGTTCAAGCTTCAAGAAATGCTCAAGGGCGGATCTCCGGTGATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCCAAAAATGCCACAAATTCCGATGATTTAATCTGGGTGGAATCGCCGGACACCTTCTGCTTCCATTTGTGGAATGCTTGGGAGGAGCCGGAGACAGAGGAGGTCGTGGTTATTGGGTCTTGCATGACGCCGCCGGATTCCATTTTCAACGAGTGCGATGAGAATTTGAAAAGCGTACTGTCGGAAATTCGGCTGAATTTGAGAACGGGGAAATCCACGCGCCGCCCAATTATCAGCGATGAGAAGGAGCAGGTGAATCTAGAAGCCGGAATGGTGAATAGAAACCGTCTGGGGCGGAAAACCCGATATGCGTATCTTGCGATTGCAGAGCCATGGCCGAAGGTTTCGGGATTTGCGAAGGTTGATCTAGTAACAGGGGAGGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGAGGAGAGCCGTTTTTCTTACAGAAAGAGGGGAATTCAGGGGAGAAGGAAGACGAAGGGTACATTTTAGCTTTCGTCCACGATGAAAGGACATGGAAATCAGAGTTACAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAGTGAAGCTGCCATCCAGAGTACCCTATGGCTTTCACGGGACATTCATCGACGCAAAACACTTGCAAAATCAAGCTTAA

Protein sequence

MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA
Homology
BLAST of CmaCh03G014040 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 1.8e-259
Identity = 429/547 (78.43%), Postives = 491/547 (89.76%), Query Frame = 0

Query: 42  IHSALHS-PSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPL 101
           IHSAL + P   + P + P F         S+WNF QK+A+ ALD+ ESAL+S EL++PL
Sbjct: 43  IHSALLTLPKQNNTPKNQPQFQ-------TSHWNFFQKAAAKALDIVESALVSRELQNPL 102

Query: 102 PKTADPRVQISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDG 161
           PKTADPRVQI+GNFAPVPEQ V++NLPVTGTIPDCINGVY+RNGANPLFEP AGHHLFDG
Sbjct: 103 PKTADPRVQIAGNFAPVPEQSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDG 162

Query: 162 DGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARG 221
           DGMVHAVT+ +GS SY+CRFT+T+RL QE++LG P+FPKAIGELHGHSGIARLLLFYARG
Sbjct: 163 DGMVHAVTVENGSVSYSCRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARG 222

Query: 222 MFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDST 281
           +FGLVDH  GTGVANAGLV+FN+RLLAMSEDD+PYHV++ PSGDL+TVGRY+F+ QL ST
Sbjct: 223 VFGLVDHSHGTGVANAGLVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKST 282

Query: 282 MIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITEN 341
           MIAHPK+DPVS EL+ALSYDVV+KPYLK F+FSPDG KS DVEIPL  PTMMHDFAITEN
Sbjct: 283 MIAHPKIDPVSGELFALSYDVVQKPYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITEN 342

Query: 342 FVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLW 401
           +VVIPDQQVVFKLQEM+KGGSPVIYDKNKKSRFGILPKNA NS+++IWVES +TFCFHLW
Sbjct: 343 YVVIPDQQVVFKLQEMIKGGSPVIYDKNKKSRFGILPKNAENSENIIWVESAETFCFHLW 402

Query: 402 NAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQ 461
           NAWEEPET+EV+VIGSCMTPPDSIFNEC+ENLKSVLSEIRLNL+TG+STRR ++S   +Q
Sbjct: 403 NAWEEPETDEVIVIGSCMTPPDSIFNECNENLKSVLSEIRLNLKTGESTRRQLLS-PSDQ 462

Query: 462 VNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFF 521
           VNLEAGMVNRN+LGRKT++AYLAIAEPWPKVSGFAKVDL TGE+KKHIYG +++GGEP F
Sbjct: 463 VNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLF 522

Query: 522 LQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFID 581
           L +  NS EKED+GYILAF HDE+TWKSELQIVNAM L++EATVKLPSRVPYGFHGTFI 
Sbjct: 523 LPRNVNS-EKEDDGYILAFCHDEKTWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFIS 580

Query: 582 AKHLQNQ 588
           +K LQNQ
Sbjct: 583 SKDLQNQ 580

BLAST of CmaCh03G014040 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 884.8 bits (2285), Expect = 5.4e-256
Identity = 435/596 (72.99%), Postives = 493/596 (82.72%), Query Frame = 0

Query: 7   SPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPNF----- 66
           S P     GF  ++ SI+  KN  H RQ  N    I+S+L +P +LHFP  S N+     
Sbjct: 18  SMPSSKEFGF--ASNSISLLKN-QHNRQSLN----INSSLQAPPILHFPKQSSNYQTPKN 77

Query: 67  ---------HDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISG 126
                    ++N   S  S WN +QK+A++ALD  ESAL  HEL HPLPKTADPRVQISG
Sbjct: 78  NTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISG 137

Query: 127 NFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDG 186
           NFAPVPE PV  +LPVTG IP C+ GVY+RNGANPLFEPTAGHH FDGDGMVHAV   +G
Sbjct: 138 NFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNG 197

Query: 187 SASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTG 246
           SASYACRFT+T+RL QE+ LGRP+FPKAIGELHGHSGIARL+LFYARG+FGLVDH KGTG
Sbjct: 198 SASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTG 257

Query: 247 VANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSR 306
           VANAGLVYFN+RLLAMSEDDLPYHV++TP+GDL+T GR+DF+ QL STMIAHPK+DPVS 
Sbjct: 258 VANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSG 317

Query: 307 ELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFK 366
           EL+ALSYDV++KPYLKYFRFS +G KS DVEIP++ PTMMHDFAITENFVVIPDQQVVFK
Sbjct: 318 ELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFK 377

Query: 367 LQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVV 426
           + EM++GGSPV+YDKNK SRFGIL K A +  DL WVE PD FCFHLWNAWEE ET+E+V
Sbjct: 378 MSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIV 437

Query: 427 VIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNR 486
           VIGSCMTPPDSIFNECDE LKSVLSEIRLNL+TGKSTR+ II +  EQVNLEAGMVNRN+
Sbjct: 438 VIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNK 497

Query: 487 LGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKED 546
           LGRKT YAYLAIAEPWPKVSGFAKV+L TGEV+K IYG  K+GGEP FL ++ NS E ED
Sbjct: 498 LGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKE-ED 557

Query: 547 EGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           +GYILAFVHDE+ WKSELQIVNAM LK+EATVKLPSRVPYGFHGTFI+A  L NQA
Sbjct: 558 DGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA 605

BLAST of CmaCh03G014040 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 1.6e-244
Identity = 410/551 (74.41%), Postives = 460/551 (83.48%), Query Frame = 0

Query: 42  IHSALHSPSVLHFPNHSPNFHDNLIK-----SPNSNWNFLQKSASIALDMAESALLSHEL 101
           + SALH+P  LHFP  S N    ++K     S     N  Q++A+ ALD AE  L+SHE 
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEK 109

Query: 102 RHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHH 161
            HPLPKTADP VQI+GNFAPV EQPV+ NLPV G +PD I GVY+RNGANPL EP  GHH
Sbjct: 110 LHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHH 169

Query: 162 LFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLF 221
            FDGDGMVHAV    GSASYACRFTQT R  QE+QLGRP+FPKAIGELHGH+GIARL+LF
Sbjct: 170 FFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 229

Query: 222 YARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQ 281
           YAR   G+VD   GTGVANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ Q
Sbjct: 230 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 289

Query: 282 LDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFA 341
           L+STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSPDG KS DVEI L  PTMMHDFA
Sbjct: 290 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 349

Query: 342 ITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFC 401
           ITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A +S ++ W+++PD FC
Sbjct: 350 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 409

Query: 402 FHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISD 461
           FHLWNAWEEPET+EVVVIGSCMTPPDSIFNE DENLKSVLSEIRLNL+TG+STRRPIIS+
Sbjct: 410 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 469

Query: 462 EKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGG 521
           E +QVNLEAGMVNRN LGRKT++AYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++GG
Sbjct: 470 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGG 529

Query: 522 EPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHG 581
           EP FL  EG    +EDEGYIL FVHDE+TWKSELQIVNA+ L+VEATVKLPSRVPYGFHG
Sbjct: 530 EPLFLPGEGG---EEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHG 589

Query: 582 TFIDAKHLQNQ 588
           TFI A  L  Q
Sbjct: 590 TFIGADDLAKQ 597

BLAST of CmaCh03G014040 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 1.4e-243
Identity = 399/516 (77.33%), Postives = 457/516 (88.57%), Query Frame = 0

Query: 73  WNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTI 132
           WNFLQK+A+  LDM E+AL+SHE +HPLPKTADP+VQI+GNFAPVPE      LPV G I
Sbjct: 105 WNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQALPVVGKI 164

Query: 133 PDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQL 192
           P CI+GVY+RNGANPL+EP AGHH FDGDGMVHAV   +G+ASYACRFT+TQRLAQE+ L
Sbjct: 165 PKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGAASYACRFTETQRLAQEKSL 224

Query: 193 GRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDD 252
           GRP+FPKAIGELHGHSGIARLLLFYAR +F LVD   G GVANAGLVYFN+ LLAMSEDD
Sbjct: 225 GRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMSEDD 284

Query: 253 LPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRF 312
           LPYHVRIT +GDL TVGRYDFN QL+STMIAHPK+DPV+ +L+ALSYDVV+KPYLKYFRF
Sbjct: 285 LPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKPYLKYFRF 344

Query: 313 SPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSR 372
           S DG+KS DVEIPL+ PTMMHDFAITENFVV+PDQQVVFKL EM+ GGSPV+YDKNK SR
Sbjct: 345 SADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVYDKNKTSR 404

Query: 373 FGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENL 432
           FGIL KNA +++ + W+++P+ FCFHLWNAWEEPET+E+VVIGSCMTP DSIFNECDE+L
Sbjct: 405 FGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIFNECDESL 464

Query: 433 KSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVS 492
           KSVLSEIRLNLRTGKSTRRPIISD  EQVNLEAGMVNRN+LGRKT++AYLA+AEPWPKVS
Sbjct: 465 KSVLSEIRLNLRTGKSTRRPIISD-AEQVNLEAGMVNRNKLGRKTQFAYLALAEPWPKVS 524

Query: 493 GFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQI 552
           GFAKVDL +GEV+K++YG  KFGGEP FL     +GE+E +GYILAFVHDE+ WKSELQI
Sbjct: 525 GFAKVDLFSGEVQKYMYGEEKFGGEPLFLP----NGEEEGDGYILAFVHDEKEWKSELQI 584

Query: 553 VNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           VNA +LK+EA++KLPSRVPYGFHGTFI +K L+ QA
Sbjct: 585 VNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQA 615

BLAST of CmaCh03G014040 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 2.2e-241
Identity = 402/555 (72.43%), Postives = 470/555 (84.68%), Query Frame = 0

Query: 34  QPSNHNAVIHSALHSPSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLS 93
           +P       +S   +P++L+FPN+ P+ +  + +   S WN LQ++AS ALD AE+ALL 
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNY-PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLR 97

Query: 94  HELRHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTA 153
            E   PLPKT DPR QISGN+APVPEQ VK +L V G IPDCI+GVYLRNGANPLFEP +
Sbjct: 98  RERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVS 157

Query: 154 GHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARL 213
           GHHLFDGDGMVHAV I +G ASY+CRFT+T+RL QE+QLG PIFPKAIGELHGHSGIARL
Sbjct: 158 GHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARL 217

Query: 214 LLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDF 273
           +LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSEDDLPY VR+T +GDLET+GR+DF
Sbjct: 218 MLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDF 277

Query: 274 NTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMH 333
           + QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYF+FSP+G KS DVEIPL +PTMMH
Sbjct: 278 DGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMH 337

Query: 334 DFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPD 393
           DFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGILP+NA ++ +++WVESP+
Sbjct: 338 DFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPE 397

Query: 394 TFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPI 453
           TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNECDE L SVLSEIRLNL+TGKSTRR I
Sbjct: 398 TFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTI 457

Query: 454 ISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRK 513
           I     Q+NLEAGMVNRN LGRKTRYAYLAIAEPWPKVSGFAKVDL TGEVK H YGG+K
Sbjct: 458 IPG-SVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKK 517

Query: 514 FGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYG 573
           +GGEPFFL + G   + ED+GYI++FVHDE +W+SEL IVNA+ L++EATVKLPSRVPYG
Sbjct: 518 YGGEPFFLPR-GLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYG 577

Query: 574 FHGTFIDAKHLQNQA 589
           FHGTF+++  + NQA
Sbjct: 578 FHGTFVNSADMLNQA 589

BLAST of CmaCh03G014040 vs. ExPASy TrEMBL
Match: A0A6J1IVE0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478741 PE=3 SV=1)

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

BLAST of CmaCh03G014040 vs. ExPASy TrEMBL
Match: A0A6J1EH85 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433338 PE=3 SV=1)

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVK+NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of CmaCh03G014040 vs. ExPASy TrEMBL
Match: A0A2Z4GT57 (9-cis-epoxycarotenoid dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 583/588 (99.15%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNA+IHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNALIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVK+NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSR LYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRGLYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKS+DVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSQDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of CmaCh03G014040 vs. ExPASy TrEMBL
Match: A0A1L5JHV5 (9-cis-epoxycarotenoid dioxygenase NCED2 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.7e-302
Identity = 517/601 (86.02%), Postives = 547/601 (91.01%), Query Frame = 0

Query: 2   AASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNA--VIHSALHSPSVLHFPNHSP 61
           A SS S   LD   F NSTKSI  FKNV   +Q +N NA  V+H ALHSPSVLHFPN + 
Sbjct: 4   APSSSSSSLLDLGAFSNSTKSIR-FKNVKISQQSNNRNAVLVVHCALHSPSVLHFPNQT- 63

Query: 62  NFHDNLI----------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQ 121
           N+H  L+          K PNSNWNFLQK+AS+A D+ ESALLSHEL+HPLPKTADPRVQ
Sbjct: 64  NYHKKLVLKEDTNTNKPKPPNSNWNFLQKTASMAFDIVESALLSHELQHPLPKTADPRVQ 123

Query: 122 ISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTI 181
           ISGNFAPVPEQPVK+NLP+TGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAV+I
Sbjct: 124 ISGNFAPVPEQPVKHNLPITGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVSI 183

Query: 182 NDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKK 241
           N  +ASYACRFT+TQRL QE++LGR +FPKAIGELHGHSGIARLLLFY RG+FGLVDHK 
Sbjct: 184 NGATASYACRFTETQRLVQERRLGRSVFPKAIGELHGHSGIARLLLFYGRGLFGLVDHKN 243

Query: 242 GTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDP 301
           GTGVANAGLVYFNDRLLAMSEDDLPYHVRIT SGDLETVGRYDF  QL STMIAHPKVDP
Sbjct: 244 GTGVANAGLVYFNDRLLAMSEDDLPYHVRITASGDLETVGRYDFKMQLKSTMIAHPKVDP 303

Query: 302 VSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQV 361
           VSRELYALSYDVVRKPYLKYFRFSPDG KSKD+EIPL TPTMMHDFAITENFVVIPDQQV
Sbjct: 304 VSRELYALSYDVVRKPYLKYFRFSPDGTKSKDLEIPLHTPTMMHDFAITENFVVIPDQQV 363

Query: 362 VFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETE 421
           VFKLQEM+KGGSPVIYDKNKKSRFGILPKNA++S+DLIWVESPDTFCFHLWNAWEEPET+
Sbjct: 364 VFKLQEMVKGGSPVIYDKNKKSRFGILPKNASDSNDLIWVESPDTFCFHLWNAWEEPETD 423

Query: 422 EVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVN 481
           EVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDE++QVNLEAGMVN
Sbjct: 424 EVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEEQQVNLEAGMVN 483

Query: 482 RNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNS-- 541
           RNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEG S  
Sbjct: 484 RNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGKSEK 543

Query: 542 GEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQ 589
            E+EDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DA +LQNQ
Sbjct: 544 EEEEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDANNLQNQ 602

BLAST of CmaCh03G014040 vs. ExPASy TrEMBL
Match: A0A1S3AZU0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103484361 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.0e-301
Identity = 510/598 (85.28%), Postives = 551/598 (92.14%), Query Frame = 0

Query: 4   SSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHS----- 63
           ++P  P LD+LGF NSTKS     N+NH++  +  N++IHSALHSPSVL FPN +     
Sbjct: 2   TAPPSPLLDHLGFLNSTKSNFPNLNINHLKSITTQNSIIHSALHSPSVLRFPNQTTTTPP 61

Query: 64  -PNFHDNLI----KSPNS-NWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISG 123
            PNFH +L     KSP + NWN LQK+ASIALDM ESAL+SHEL+HPLPKTADPRVQISG
Sbjct: 62  PPNFHKSLSLKHQKSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADPRVQISG 121

Query: 124 NFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDG 183
           NFAPVPEQPVK+NLPVTGTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVHAVTIN G
Sbjct: 122 NFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINHG 181

Query: 184 SASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTG 243
           SASYACRFTQTQRL QE++LGRPIFPKAIGELHGHSGIARLLLFYARG+FGLVDHKKGTG
Sbjct: 182 SASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTG 241

Query: 244 VANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSR 303
           VANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDF+ QL STMIAHPKVDP+S+
Sbjct: 242 VANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPKVDPISK 301

Query: 304 ELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFK 363
           ELYALSYDVVRKPYLKYFRFSP+GIKSKDVEIP++TPTMMHDFAITENFVVIPDQQVVFK
Sbjct: 302 ELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFK 361

Query: 364 LQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVV 423
           LQEM+KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPETEEVV
Sbjct: 362 LQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVV 421

Query: 424 VIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNR 483
           VIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMVN+ R
Sbjct: 422 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNKTR 481

Query: 484 LGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNS--GEK 543
           +GRK+RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL K+ NS   E+
Sbjct: 482 VGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDENSKAEEE 541

Query: 544 EDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           EDEGYILAFVHDERTWKSE+QIVNA DLKVEAT+KLPSRVPYGFHGTF+ + +LQNQA
Sbjct: 542 EDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNLQNQA 599

BLAST of CmaCh03G014040 vs. NCBI nr
Match: XP_022978939.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

BLAST of CmaCh03G014040 vs. NCBI nr
Match: XP_022926148.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVK+NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of CmaCh03G014040 vs. NCBI nr
Match: KAG6581433.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034725.1 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 585/588 (99.49%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVN IRQPSNHNAVIHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVK+NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of CmaCh03G014040 vs. NCBI nr
Match: XP_023543156.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita pepo subsp. pepo] >AWW05326.1 9-cis-epoxycarotenoid dioxygenase [Cucurbita pepo])

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 583/588 (99.15%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPN 60
           MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNA+IHSALHSPSVLHFPNHSPN
Sbjct: 1   MAASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNALIHSALHSPSVLHFPNHSPN 60

Query: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120
           FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ
Sbjct: 61  FHDNLIKSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQ 120

Query: 121 PVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180
           PVK+NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF
Sbjct: 121 PVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRF 180

Query: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240
           TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY
Sbjct: 181 TQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVY 240

Query: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYD 300
           FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSR LYALSYD
Sbjct: 241 FNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRGLYALSYD 300

Query: 301 VVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360
           VVRKPYLKYFRFSPDGIKS+DVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG
Sbjct: 301 VVRKPYLKYFRFSPDGIKSQDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGG 360

Query: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420
           SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP
Sbjct: 361 SPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTP 420

Query: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480
           PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA
Sbjct: 421 PDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYA 480

Query: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540
           YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV
Sbjct: 481 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAFV 540

Query: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTF+DAKHLQNQA
Sbjct: 541 HDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

BLAST of CmaCh03G014040 vs. NCBI nr
Match: XP_038883946.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1061.2 bits (2743), Expect = 3.2e-306
Identity = 521/600 (86.83%), Postives = 552/600 (92.00%), Query Frame = 0

Query: 1   MAASSP--SPPCLDYLGFHNSTKS----INGFKNVNHIRQPSNHNAVIHSALHSPSVLHF 60
           MAAS P  S   LD+L F NST+S     N   NV  I Q S HN +IHSALHSPSVLHF
Sbjct: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61

Query: 61  PNHSPNFHDNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 120
           PN +PNFH+NL       KSP SNWN LQK+AS+AL M ESAL+SHEL+HPLPKTADPRV
Sbjct: 62  PNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRV 121

Query: 121 QISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 180
           QISGNFAPVPEQPVK+NLPVTGTIPDCI+GVYLRNGANPLF+PTAGHHLFDGDGMVHAVT
Sbjct: 122 QISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVT 181

Query: 181 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 240
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 182 INGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 241

Query: 241 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 300
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QL STMIAHPKVD
Sbjct: 242 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVD 301

Query: 301 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 360
           P+S+ELYALSYDVVRKPYLKYFRFSPDG+KSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 302 PISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 361

Query: 361 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 420
           VVFKLQEM+KGGSPVIYDK KKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 362 VVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 421

Query: 421 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 480
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQ+NLEAGMV
Sbjct: 422 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMV 481

Query: 481 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 540
           NRNRLGRKTR+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNS 
Sbjct: 482 NRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSK 541

Query: 541 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           E EDEGYILAFVHDERTWKSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTF+ +  LQNQA
Sbjct: 542 E-EDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 600

BLAST of CmaCh03G014040 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 846.7 bits (2186), Expect = 1.2e-245
Identity = 410/551 (74.41%), Postives = 460/551 (83.48%), Query Frame = 0

Query: 42  IHSALHSPSVLHFPNHSPNFHDNLIK-----SPNSNWNFLQKSASIALDMAESALLSHEL 101
           + SALH+P  LHFP  S N    ++K     S     N  Q++A+ ALD AE  L+SHE 
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEK 109

Query: 102 RHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHH 161
            HPLPKTADP VQI+GNFAPV EQPV+ NLPV G +PD I GVY+RNGANPL EP  GHH
Sbjct: 110 LHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHH 169

Query: 162 LFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLF 221
            FDGDGMVHAV    GSASYACRFTQT R  QE+QLGRP+FPKAIGELHGH+GIARL+LF
Sbjct: 170 FFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 229

Query: 222 YARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQ 281
           YAR   G+VD   GTGVANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ Q
Sbjct: 230 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 289

Query: 282 LDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFA 341
           L+STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSPDG KS DVEI L  PTMMHDFA
Sbjct: 290 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 349

Query: 342 ITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFC 401
           ITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A +S ++ W+++PD FC
Sbjct: 350 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 409

Query: 402 FHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISD 461
           FHLWNAWEEPET+EVVVIGSCMTPPDSIFNE DENLKSVLSEIRLNL+TG+STRRPIIS+
Sbjct: 410 FHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISN 469

Query: 462 EKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGG 521
           E +QVNLEAGMVNRN LGRKT++AYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++GG
Sbjct: 470 EDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGG 529

Query: 522 EPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHG 581
           EP FL  EG    +EDEGYIL FVHDE+TWKSELQIVNA+ L+VEATVKLPSRVPYGFHG
Sbjct: 530 EPLFLPGEGG---EEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHG 589

Query: 582 TFIDAKHLQNQ 588
           TFI A  L  Q
Sbjct: 590 TFIGADDLAKQ 597

BLAST of CmaCh03G014040 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 836.3 bits (2159), Expect = 1.6e-242
Identity = 402/555 (72.43%), Postives = 470/555 (84.68%), Query Frame = 0

Query: 34  QPSNHNAVIHSALHSPSVLHFPNHSPNFHDNLIKSPNSNWNFLQKSASIALDMAESALLS 93
           +P       +S   +P++L+FPN+ P+ +  + +   S WN LQ++AS ALD AE+ALL 
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNY-PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLR 97

Query: 94  HELRHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTA 153
            E   PLPKT DPR QISGN+APVPEQ VK +L V G IPDCI+GVYLRNGANPLFEP +
Sbjct: 98  RERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVS 157

Query: 154 GHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARL 213
           GHHLFDGDGMVHAV I +G ASY+CRFT+T+RL QE+QLG PIFPKAIGELHGHSGIARL
Sbjct: 158 GHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARL 217

Query: 214 LLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDF 273
           +LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSEDDLPY VR+T +GDLET+GR+DF
Sbjct: 218 MLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDF 277

Query: 274 NTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMH 333
           + QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYF+FSP+G KS DVEIPL +PTMMH
Sbjct: 278 DGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMH 337

Query: 334 DFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPD 393
           DFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGILP+NA ++ +++WVESP+
Sbjct: 338 DFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPE 397

Query: 394 TFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPI 453
           TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNECDE L SVLSEIRLNL+TGKSTRR I
Sbjct: 398 TFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTI 457

Query: 454 ISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRK 513
           I     Q+NLEAGMVNRN LGRKTRYAYLAIAEPWPKVSGFAKVDL TGEVK H YGG+K
Sbjct: 458 IPG-SVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKK 517

Query: 514 FGGEPFFLQKEGNSGEKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYG 573
           +GGEPFFL + G   + ED+GYI++FVHDE +W+SEL IVNA+ L++EATVKLPSRVPYG
Sbjct: 518 YGGEPFFLPR-GLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYG 577

Query: 574 FHGTFIDAKHLQNQA 589
           FHGTF+++  + NQA
Sbjct: 578 FHGTFVNSADMLNQA 589

BLAST of CmaCh03G014040 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 810.8 bits (2093), Expect = 7.0e-235
Identity = 381/515 (73.98%), Postives = 451/515 (87.57%), Query Frame = 0

Query: 74  NFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAPVPEQPVKYNLPVTGTIP 133
           N  QK+A+IA+D AE AL+SHE   PLPKTADPRVQI+GN++PVPE  V+ NL V GTIP
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 134 DCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASYACRFTQTQRLAQEQQLG 193
           DCI+GVY+RNGANP+FEPTAGHHLFDGDGMVHAV I +GSASYACRFT+T+RL QE++LG
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLG 189

Query: 194 RPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDL 253
           RP+FPKAIGELHGHSGIARL+LFYARG+ GL++++ G GVANAGLVYFN+RLLAMSEDDL
Sbjct: 190 RPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDL 249

Query: 254 PYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFS 313
           PY ++IT +GDL+TVGRYDF+ QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYFRFS
Sbjct: 250 PYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRFS 309

Query: 314 PDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPVIYDKNKKSRF 373
           PDG+KS ++EIPL+TPTM+HDFAITENFVVIPDQQVVFKL EM+ G SPV++D  K SR 
Sbjct: 310 PDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSRL 369

Query: 374 GILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECDENLK 433
           GI+PK+AT +  +IWV SP+TFCFHLWNAWE PETEE+VVIGSCM+P DSIFNE DE+L+
Sbjct: 370 GIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESLR 429

Query: 434 SVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRYAYLAIAEPWPKVSG 493
           SVLSEIR+NLRT K+TRR ++ +  E VNLE GMVNRNRLGRKTR+A+LAIA PWPKVSG
Sbjct: 430 SVLSEIRINLRTRKTTRRSLLVN--EDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSG 489

Query: 494 FAKVDLVTGEVKKHIYGGRKFGGEPFFLQ-KEGNSGEKEDEGYILAFVHDERTWKSELQI 553
           FAKVDL TGE+KK+IYGG K+GGEPFFL    GN  E ED+GYI   VHDE T  SELQI
Sbjct: 490 FAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQI 549

Query: 554 VNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQ 588
           +NA++LK+EAT+KLPSRVPYGFHGTF+D+  L +Q
Sbjct: 550 INAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of CmaCh03G014040 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 790.4 bits (2040), Expect = 9.8e-229
Identity = 399/593 (67.28%), Postives = 474/593 (79.93%), Query Frame = 0

Query: 3   ASSPSPPCLDYLGFHNSTKSINGFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPNFH 62
           +SS S P L  L F +ST          ++    N  + + S+L SP+    P+     +
Sbjct: 72  SSSSSRPKLQSLSF-SSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPSWKKLCN 131

Query: 63  D--NLI-KSPNSN--WNFLQKSASIALDMAESALLSHE-LRHPLPKTADPRVQISGNFAP 122
           D  NLI K+ N N   N +Q++A++ LD  E+A++SHE  RHP PKTADP VQI+GNF P
Sbjct: 132 DVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFP 191

Query: 123 VPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASY 182
           VPE+PV +NLPVTGT+P+CI GVY+RNGANPL +P +GHHLFDGDGMVHAV  ++GS SY
Sbjct: 192 VPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFDNGSVSY 251

Query: 183 ACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANA 242
           ACRFT+T RL QE++ GRP+FPKAIGELHGH GIA+L+LF  RG+FGLVD   G GVANA
Sbjct: 252 ACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANA 311

Query: 243 GLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYA 302
           GLVYFN  LLAMSEDDLPYHV++T +GDLET GRYDF+ QL STMIAHPK+DP +REL+A
Sbjct: 312 GLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFA 371

Query: 303 LSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEM 362
           LSYDVV KPYLKYFRF+ DG KS DVEIPL  PTM+HDFAITENFVVIPDQQVVF+L EM
Sbjct: 372 LSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEM 431

Query: 363 LKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 422
           ++GGSPV+YD+ KKSRFGIL KNA ++  + W+E PD FCFHLWN+WEEPET+EVVVIGS
Sbjct: 432 IRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGS 491

Query: 423 CMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRK 482
           CMTPPDSIFNE DE L+SVLSEIRLNL+TG+STRRP+IS   EQVNLEAGMVNRN LGRK
Sbjct: 492 CMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVIS---EQVNLEAGMVNRNLLGRK 551

Query: 483 TRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGE-KEDEGY 542
           TRYAYLA+ EPWPKVSGFAKVDL TGE++K+IYG  K+GGEP FL     SG+ +ED GY
Sbjct: 552 TRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP----SGDGEEDGGY 611

Query: 543 ILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 589
           I+ FVHDE   KSELQ++NA+++K+EATV LPSRVPYGFHGTFI  + L  QA
Sbjct: 612 IMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

BLAST of CmaCh03G014040 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.1e-179
Identity = 309/528 (58.52%), Postives = 389/528 (73.67%), Query Frame = 0

Query: 66  IKSPNSNWNFLQKSASIALDMAESAL-LSHELRHPLPKTADPRVQISGNFAPVPEQPVKY 125
           +K    N N LQK A+  LD  ES++ +  E   PLPK  DP VQ+SGNFAPV E PV+ 
Sbjct: 56  LKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQN 115

Query: 126 NLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTIN-DGSASYACRFTQT 185
            L V G IP C+ GVY+RNGANP+F P AGHHLFDGDGM+HAV+I  D   SY+CR+T+T
Sbjct: 116 GLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKT 175

Query: 186 QRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANAGLVYFND 245
            RL QE  LGR +FPK IGELHGHSG+ARL LF AR   GLVD  +G GVANAG+V+FN 
Sbjct: 176 NRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNG 235

Query: 246 RLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYALSYDVVR 305
           RLLAMSEDDLPY V+I   GDLET+GR+ F+ Q+DS++IAHPKVD  + +L+ LSY+V++
Sbjct: 236 RLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLK 295

Query: 306 KPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEMLKGGSPV 365
           KP+L+Y +F+  G K++DVEI L  PTM+HDFAITENFVVIPDQQ+VFKL EM++GGSPV
Sbjct: 296 KPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPV 355

Query: 366 IYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEE----VVVIGSCMT 425
           IY K K +RFG+L K      D+ WV+ PD FCFHLWNAWEE  TEE    +VVIGSCM+
Sbjct: 356 IYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPVIVVIGSCMS 415

Query: 426 PPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRKTRY 485
           PPD+IF+E  E  +  LSEIRLN+RT +S R+ I++     VNLEAG +NR+ +GRK+++
Sbjct: 416 PPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVTG----VNLEAGHINRSYVGRKSQF 475

Query: 486 AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSGEKEDEGYILAF 545
            Y+AIA+PWPK SG AKVD+  G V +  YG  +FGGEP F+  EG    +ED+GY++ F
Sbjct: 476 VYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFV-PEGEG--EEDKGYVMGF 535

Query: 546 VHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQ 588
           V DE   +SE  +V+A D+K  A V+LP RVPYGFHGTF+    L+ Q
Sbjct: 536 VRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
K4CJJ11.8e-25978.439-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
O240235.4e-25672.999-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
Q9LRR71.6e-24474.419-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9M6E81.4e-24377.339-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
Q9C6Z12.2e-24172.43Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A6J1IVE00.0e+00100.009-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A6J1EH850.0e+0099.669-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A2Z4GT570.0e+0099.159-cis-epoxycarotenoid dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A1L5JHV56.7e-30286.029-cis-epoxycarotenoid dioxygenase NCED2 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A1S3AZU02.0e-30185.289-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3... [more]
Match NameE-valueIdentityDescription
XP_022978939.10.0e+00100.009-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita maxima][more]
XP_022926148.10.0e+0099.669-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata][more]
KAG6581433.10.0e+0099.499-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyr... [more]
XP_023543156.10.0e+0099.159-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita pepo subs... [more]
XP_038883946.13.2e-30686.839-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G14440.11.2e-24574.41nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.11.6e-24272.43nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.17.0e-23573.98nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G78390.19.8e-22967.28nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G24220.11.1e-17958.52nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 114..579
e-value: 3.2E-116
score: 389.3
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 62..588
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 62..588

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G014040.1CmaCh03G014040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen