CmaCh03G011030 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G011030
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial
Location	Cma_Chr03: 7532983 .. 7536996 (-)
RNA-Seq Expression	CmaCh03G011030
Synteny	CmaCh03G011030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGCCAACAAGCTAGTGCGATAGCTACTTATCCGATTGACATTGGAGTCTTAAATTAGATAAGAATTCGTTTAATGGAATTGGCAAAATCTGTCCAAGGGCAACAAACTTCTCAATGTCCCTCTGACCCTTAATCTATATTACGAAACCCAACGACCCAATTCTTCTGCGCGGTGTTCAAGACTCGATCTGAAGCATTCTCCGTCCAGCGATGGCGTTTCATGTGAGAAAAGCAACGAAGATCATCCCCCATTTTAGAAACGGGATGCGTCTCTTGGGTTCGCTTCGCGAATCTCCATTTGCAGTTCATAAGATCGGAAACAGAGTTTTGGGGTTCGAAAATATGCTTAACCATTTGTCTGCTCATCGTTTCAAATCGACTGAAGTGGAGAAACAAATAGATTCTGTCAACGATGATCGTGACAATCAGGTAGAATGCACTCTGATTCGGTCAACTTTGATTGTCTTCTAATTTCATGTTTTGTATTTTTTGTTTTTATAATCCCTAATCGTTTTTAGATAAGATTCCTCTTAATTTTTTAATCTTCGTTTGAGGATTGTGTGTTTGCCGAGACTGCATCTAAATGATTATTTGTTGACAATGTAGTTTTAGACTTTGAACTGTAGTTTTCCTTTCCATTCTGCTTGTCAACTCCTTCCCCCTTGTTCTTTGAAAGACTTCGAGAGGAATCTCTATAAGAACAGAGAGAGATTTAAGGTAAGACATTGAGGGGAACAGATTATTCCTTAATCTTTTGTGTATTTTAGTGGCTCAAACTCCCAAGTTACAGGTCATTATGGTATATTCCATTTGTGTAGTCTGGAAGATGGAAAATGAGTTCATGTAAAGGAGTTTACTTTTGGGATTGATTGAGTTCATCCAGAAAGTTCCCCTTGAACTGTCCTGGGTCCACCCATCTCGCTGGTTGGTTCGTGTAGAAGAAGTTAGACCCCCGAATTCTAAATCTCCCATTGTTGCTACTATGTTAGATGAACCTGAAATCCCGATTCCTAAATTACAAGCGTAATGGCTTTTCCACCATTGTTGCTTGCAGCCTTCTTCAAGCTCGGGTTATGGAAGAAGGAACCTGTATTCTAGCAATGATGCAATTCAATGCAAATCCAATAGGTAATTACAAAAGGACCTTGTGACAGAAGTCTAAAGCGAAACATAGAATCTAAGAAGAGACCAATCATAGTTACGAATTCGCTCACGCCTCTCGACGATTCTATTGTTTTTCCCACACCAAAGACCTCATAATAAAGTGGAACAAATCTCGAGCATAAATTATTCAAAAAGTAGAGATTCCTCTCAAAGTCTCACCTTAAATCTCTCTGTGTTCCTCTTACCAACTGTGAACGAGTTCATAATATTCAATGTCATTCAATTGTGTACAAGTTCCTGATATGATTTGTTGGAAGTGAAACTTGCATTTCTTGTACAAGGTTTTGAACTTTCCAGGAGGAAAGGTTAAATTTACTCCTCAATTGAGTTTCGTCCCTGAGCGCGAGGAGGAGCGGATACCCTGCTTTCGTGTTCTTGATGACAACGGGCAGCCTAGTATGTACAGTAATTTTACACAGGTAATTTATGCTGAGCATGTCCCAAGTTCATGTGAAACGATAGGCATTTATGAACTTGATGGTACTTTCATTTAGGTGAGTAAGGAGATTGCCATGAAGATGTATAATGACATGGTTACTCTTCAAACAATGGACACTATCTTTTATGAAGCGCAAAGGCAAGGAAGAATTTCCTTCTATGTGACCTCCATCGGAGAAGAAGCAATCAACATTGCTTCTGCTGCAGCATTGTCATTGGATGACCTCATTGTCCCACAGGTTACTTCATACCTCCCCCTCATCATCCTCCTTAGTAAAGCACATTCAAATCCTCACATGGAAAGAATATTATTTGCTTGCACTAATATCTTACCTAATTAGTCACATGTGAGATCGCACATTGGTTGGAGAGGGGAACAAAACATTTCTTATTAGGGTGTGGAAACCTCTCCTAGTAGACGCGTTTTAAAATCTTGAGGGGAAGCCTGGGAGGGAAAGCCCAAAGAGGACAATATCTGCTAGTGGTGAGCTTGGACTATTACAAATGGTATCAGAGGCAGACATTGAACAGTACGCCAACGAGGACGCTTGGGGTGTGGATTATGAGATCGCACATTGGTTGGAGAGGGGAAAAAATCACTCCTTATAAGGGTGTGGAAACTTCTTCCTAGTAGATGCGTTTTAAAATCTTGAGGGAAAGCCCGAGAGGAAAAGCCGAAAGAGGACAATATCTGTTAACGGTGGGCTTAAGTTGTTACACCACAATTGTGTGATTGATAGTTCTTATTGTAACTCTCCTACGTCATACAGTACAGGGAGGCAGGAGTACTCGTATGGCGCGGTTTTACTTTGAAAGAATTTGCAAGCCAGTGCTTCGGAAACAAATATGATTATTGTAAAGGGAGGCAAATGCCAGTCCATTACGGGTCGAAGAGACACAATTACTTTACTGTAGCATCAACGATCGCGTAAGACTTGAGCTCAATTGGTCAAAAATTTTGAGCAGATCTTCCAACACTTCAACTCTTATTACTCATGTTTTACTCTAATTTGTAGTTCTCAAATCCCCCATGCCGTTGGTGCTGCATATTCAATGAAGATGGAAAAAAGAGATGCTTGTGCAGTCACTTATTTTGGAGATGGAGGAACGAGCGAGGTACAGAATACTAAGAAAAACTCGTAGTGTTCGAATTTGTTGTACTCTTCCAAGTTGCAAGTTGATTTTTGTGTTGTTTCTTGAATCATGTTACATTAGGGAGATTTCCACGCAGCTTTAAATTTTGCAGCAGTTTTGGAGGTGCCTGTGATTTTTATTTGCCGGAACAATGGATGGGCAATCAGTACTCCCATTTCAGACCAGTTTCGAAGTATCTCATTTTTCTCACTATTTACAGTTGTGAAGAATGCATTACGTTATTAGATTTTAGAGTGTTATATTGTTTTGCAGGTGATGGGGTGGTTGTAAAAGGCCGGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAACGATGCACTTGCTGTATATAATACAGTTCAAGCTGCTCGAAAAATGGCAATTCAAGAACGAAGGCCGATTTTAATTGAGGTAAGAAGGGTTCTCAATCTCTTTCTCTTTGAATGGATAACAATGGTGTTCTGATACCTTTTGTTCCATTAACAGGCTCTCACATATAGAGTTGGACACCACACGACATCAGATGACTCGACCAGGTATCGTCCGGTGGAGGAAGTGGAATGGTGGAGGGTTGCAAAGGACCCTGTCTCCAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTGATGAGGCCGAAGCAGAGCTTAGAAGTAGCCTGAGAAAACAGGTAAATTTTCAGTGCATCAACTAAACAACCTCCTTGCCATGTCTTTTCTTTTTCCTAATGAGAAACAAGAGACCATTCCATTCCAAAAAAGAGAACAAAAAGAACAACCTACGAGAACTAAATAATTTTGAAAAATACTTTCACGAGAACTAAGAAATGGTGAGAAAAGAAACAAGAATAAGCATAAAATGGTTATCAAATGAGTCGTGTACATTCTTAATTTGCTGTGTTTTTGGTTCAAACAGCTATTAGAGGCAATTCAGTTGGCAGAGAGATCTGAGAAGCCAACTGTGGCAGATGCTTTCACTGATGTGTATGATGTTCCTCCCTCCAACCTTGACGAGCAAGAGAGATGGTTGAGAAAAACAATCAAAACACACCCACAAGATTACCCATCTGACTTTCCACTGTTCTAACCTGTCTTTAGGTTTTGAAACACTCTGAGAAATATGTGAATTGGTGGCAGTGTTTATGAATAAAAGGACATAAAGGCATCCTGATGAAATAAAGCAAGTCATCCATATATGAATGGAATGTTATATCAAAGTTCTTGAATAAATTGTCAGACAAAGCTTTAAAAAACTGAATTTCATTTGAGTTTAG

mRNA sequence

AATGCCAACAAGCTAGTGCGATAGCTACTTATCCGATTGACATTGGAGTCTTAAATTAGATAAGAATTCGTTTAATGGAATTGGCAAAATCTGTCCAAGGGCAACAAACTTCTCAATGTCCCTCTGACCCTTAATCTATATTACGAAACCCAACGACCCAATTCTTCTGCGCGGTGTTCAAGACTCGATCTGAAGCATTCTCCGTCCAGCGATGGCGTTTCATGTGAGAAAAGCAACGAAGATCATCCCCCATTTTAGAAACGGGATGCGTCTCTTGGGTTCGCTTCGCGAATCTCCATTTGCAGTTCATAAGATCGGAAACAGAGTTTTGGGGTTCGAAAATATGCTTAACCATTTGTCTGCTCATCGTTTCAAATCGACTGAAGTGGAGAAACAAATAGATTCTGTCAACGATGATCGTGACAATCAGGTTTTGAACTTTCCAGGAGGAAAGGTTAAATTTACTCCTCAATTGAGTTTCGTCCCTGAGCGCGAGGAGGAGCGGATACCCTGCTTTCGTGTTCTTGATGACAACGGGCAGCCTAGTATGTACAGTAATTTTACACAGGTGAGTAAGGAGATTGCCATGAAGATGTATAATGACATGGTTACTCTTCAAACAATGGACACTATCTTTTATGAAGCGCAAAGGCAAGGAAGAATTTCCTTCTATGTGACCTCCATCGGAGAAGAAGCAATCAACATTGCTTCTGCTGCAGCATTGTCATTGGATGACCTCATTGTCCCACAGTACAGGGAGGCAGGAGTACTCGTATGGCGCGGTTTTACTTTGAAAGAATTTGCAAGCCAGTGCTTCGGAAACAAATATGATTATTGTAAAGGGAGGCAAATGCCAGTCCATTACGGGTCGAAGAGACACAATTACTTTACTGTAGCATCAACGATCGCTTCTCAAATCCCCCATGCCGTTGGTGCTGCATATTCAATGAAGATGGAAAAAAGAGATGCTTGTGCAGTCACTTATTTTGGAGATGGAGGAACGAGCGAGGGAGATTTCCACGCAGCTTTAAATTTTGCAGCAGTTTTGGAGGTGCCTGTGATTTTTATTTGCCGGAACAATGGATGGGCAATCAGTACTCCCATTTCAGACCAGTTTCGAAGTGATGGGGTGGTTGTAAAAGGCCGGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAACGATGCACTTGCTGTATATAATACAGTTCAAGCTGCTCGAAAAATGGCAATTCAAGAACGAAGGCCGATTTTAATTGAGGCTCTCACATATAGAGTTGGACACCACACGACATCAGATGACTCGACCAGGTATCGTCCGGTGGAGGAAGTGGAATGGTGGAGGGTTGCAAAGGACCCTGTCTCCAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTGATGAGGCCGAAGCAGAGCTTAGAAGTAGCCTGAGAAAACAGCTATTAGAGGCAATTCAGTTGGCAGAGAGATCTGAGAAGCCAACTGTGGCAGATGCTTTCACTGATGTGTATGATGTTCCTCCCTCCAACCTTGACGAGCAAGAGAGATGGTTGAGAAAAACAATCAAAACACACCCACAAGATTACCCATCTGACTTTCCACTGTTCTAACCTGTCTTTAGGTTTTGAAACACTCTGAGAAATATGTGAATTGGTGGCAGTGTTTATGAATAAAAGGACATAAAGGCATCCTGATGAAATAAAGCAAGTCATCCATATATGAATGGAATGTTATATCAAAGTTCTTGAATAAATTGTCAGACAAAGCTTTAAAAAACTGAATTTCATTTGAGTTTAG

Coding sequence (CDS)

ATGGCGTTTCATGTGAGAAAAGCAACGAAGATCATCCCCCATTTTAGAAACGGGATGCGTCTCTTGGGTTCGCTTCGCGAATCTCCATTTGCAGTTCATAAGATCGGAAACAGAGTTTTGGGGTTCGAAAATATGCTTAACCATTTGTCTGCTCATCGTTTCAAATCGACTGAAGTGGAGAAACAAATAGATTCTGTCAACGATGATCGTGACAATCAGGTTTTGAACTTTCCAGGAGGAAAGGTTAAATTTACTCCTCAATTGAGTTTCGTCCCTGAGCGCGAGGAGGAGCGGATACCCTGCTTTCGTGTTCTTGATGACAACGGGCAGCCTAGTATGTACAGTAATTTTACACAGGTGAGTAAGGAGATTGCCATGAAGATGTATAATGACATGGTTACTCTTCAAACAATGGACACTATCTTTTATGAAGCGCAAAGGCAAGGAAGAATTTCCTTCTATGTGACCTCCATCGGAGAAGAAGCAATCAACATTGCTTCTGCTGCAGCATTGTCATTGGATGACCTCATTGTCCCACAGTACAGGGAGGCAGGAGTACTCGTATGGCGCGGTTTTACTTTGAAAGAATTTGCAAGCCAGTGCTTCGGAAACAAATATGATTATTGTAAAGGGAGGCAAATGCCAGTCCATTACGGGTCGAAGAGACACAATTACTTTACTGTAGCATCAACGATCGCTTCTCAAATCCCCCATGCCGTTGGTGCTGCATATTCAATGAAGATGGAAAAAAGAGATGCTTGTGCAGTCACTTATTTTGGAGATGGAGGAACGAGCGAGGGAGATTTCCACGCAGCTTTAAATTTTGCAGCAGTTTTGGAGGTGCCTGTGATTTTTATTTGCCGGAACAATGGATGGGCAATCAGTACTCCCATTTCAGACCAGTTTCGAAGTGATGGGGTGGTTGTAAAAGGCCGGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAACGATGCACTTGCTGTATATAATACAGTTCAAGCTGCTCGAAAAATGGCAATTCAAGAACGAAGGCCGATTTTAATTGAGGCTCTCACATATAGAGTTGGACACCACACGACATCAGATGACTCGACCAGGTATCGTCCGGTGGAGGAAGTGGAATGGTGGAGGGTTGCAAAGGACCCTGTCTCCAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTGATGAGGCCGAAGCAGAGCTTAGAAGTAGCCTGAGAAAACAGCTATTAGAGGCAATTCAGTTGGCAGAGAGATCTGAGAAGCCAACTGTGGCAGATGCTTTCACTGATGTGTATGATGTTCCTCCCTCCAACCTTGACGAGCAAGAGAGATGGTTGAGAAAAACAATCAAAACACACCCACAAGATTACCCATCTGACTTTCCACTGTTCTAA

Protein sequence

MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPLF

Homology

[Close]

BLAST of CmaCh03G011030 vs. ExPASy Swiss-Prot
Match: Q84JL2 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g09300 PE=1 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.5e-179
Identity = 291/397 (73.30%), Postives = 354/397 (89.17%), Query Frame = 0


Query: 71  DNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQVSKEIAMKMYN 130
           ++QV++FPGGKV FTP++ F+ E ++ER+PC+RVLDDNGQ    S F QVS+E+A+K+Y+
Sbjct: 76  NHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYS 135

Query: 131 DMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQYREAGVLVWR 190
           DMVTLQ MD IFYEAQRQGR+SFY T+IGEEAINIASAAAL+  D+I PQYRE GVL+WR
Sbjct: 136 DMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWR 195

Query: 191 GFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMEK 250
           GFTL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KM+K
Sbjct: 196 GFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDK 255

Query: 251 RDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISDQFRSDGVVVK 310
           +DACAVTYFGDGGTSEGDFHAALN AAV+E PV+FICRNNGWAISTP SDQFRSDGVVVK
Sbjct: 256 KDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVK 315

Query: 311 GRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHTTSDDSTRYRP 370
           GRAYG+RSIRVDGNDALA+Y+ V  AR+MAI+E+RPILIEALTYRVGHH+TSDDSTRYR 
Sbjct: 316 GRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRS 375

Query: 371 VEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLAERSEKPTVAD 430
             E+EWW  A++P+SRFR WIESNGWWSD+ E++LRS ++K++LEA+++AE++EKP + +
Sbjct: 376 AGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQN 435

Query: 431 AFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
            F+DVYDVPPSNL EQE  +R+TI +HPQDYPSD PL
Sbjct: 436 MFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

[Close]

BLAST of CmaCh03G011030 vs. ExPASy Swiss-Prot
Match: Q9LPL5 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g21400 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 9.2e-166
Identity = 272/423 (64.30%), Postives = 349/423 (82.51%), Query Frame = 0


Query: 46  LNHLSAHRFKSTEVEKQIDSV---NDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCF 105
           L+   + R +ST VE Q D +    D+ D Q L+FPGGKV +T ++ F+PE    RIPC+
Sbjct: 48  LSPFGSLRHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCY 107

Query: 106 RVLDDNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEA 165
           RVLD++G+    S+F  VS+++A++MY  M TLQ MD IFYEAQRQGRISFY+TS+GEEA
Sbjct: 108 RVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEA 167

Query: 166 INIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKR 225
           INIASAAALS DD+++PQYRE GVL+WRGFTL+EFA+QCFGNK DY KGRQMP+HYGS R
Sbjct: 168 INIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNR 227

Query: 226 HNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVP 285
            NYFT++S IA+Q+P A G  YS+KM+K++AC VT+ GDGGTSEGDFHA LNFAAV+E P
Sbjct: 228 LNYFTISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAP 287

Query: 286 VIFICRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQ 345
           V+FICRNNGWAIST IS+QFRSDG+VVKG+AYG+RSIRVDGNDALAVY+ V++AR+MA+ 
Sbjct: 288 VVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVT 347

Query: 346 ERRPILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAE 405
           E+RP+LIE +TYRVGHH+TSDDST+YR  +E+++W+++++PV+RFRKW+E NGWWS+E E
Sbjct: 348 EQRPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDE 407

Query: 406 AELRSSLRKQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYP 465
           ++LRS+ RKQLL+AIQ AE+ EK  + + F DVYDV P NL+EQE  L++ +K  PQDYP
Sbjct: 408 SKLRSNARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYP 467

[Close]

BLAST of CmaCh03G011030 vs. ExPASy Swiss-Prot
Match: Q54M22 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=bkdA PE=3 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 9.0e-113
Identity = 188/365 (51.51%), Postives = 258/365 (70.68%), Query Frame = 0


Query: 99  IPCFRVLDDNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSI 158
           IPC+ ++D  G  S        SKE  +KMY  M+TL  MD+I Y+ QRQGRISFY+TS 
Sbjct: 64  IPCYTIMDQEGVVSKPDQDPNFSKEEVIKMYTTMLTLNVMDSILYDVQRQGRISFYMTSF 123

Query: 159 GEEAINIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHY 218
           GEEAI+I SAAAL + D I  QYRE GV +WRGFT+ +  +QC  N++D  KGRQMP+H+
Sbjct: 124 GEEAIHIGSAAALEMSDTIFAQYRETGVFMWRGFTINDIINQCCTNEHDLGKGRQMPMHF 183

Query: 219 GSKRHNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAV 278
           GS++ N  T++S + +Q+P AVG++Y+ K+     C + YFG+G  SEGDFHAA+NFAA 
Sbjct: 184 GSRKINLQTISSPLTTQLPQAVGSSYAQKLAGEKNCTIVYFGEGAASEGDFHAAMNFAAA 243

Query: 279 LEVPVIFICRNNGWAISTPISDQFRSDGVVVKG-RAYGVRSIRVDGNDALAVYNTVQAAR 338
           L  P IF CRNN WAISTP  +Q++ DG+  +G   YG+++IRVDGND  AVYN  + AR
Sbjct: 244 LSTPTIFFCRNNKWAISTPSKEQYKGDGIAGRGPNGYGMKTIRVDGNDIWAVYNVTKLAR 303

Query: 339 KMAIQERRPILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWW 398
           K+A++E+ P+LIEA+TYRVGHH+TSDDS+RYR VEE+  W+  K+P+SR R ++   GWW
Sbjct: 304 KIAVEEQVPVLIEAMTYRVGHHSTSDDSSRYRTVEEINAWKEGKNPISRLRNYMNHKGWW 363

Query: 399 SDEAEAELRSSLRKQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTH 458
           SD  E E  ++ R  + E++  AE+  KP++ + FTDVYD P  NL EQ++ L + +K +
Sbjct: 364 SDAQEKETIANARTTVRESLVNAEKQYKPSINEIFTDVYDKPTPNLIEQQKELIEHLKLY 423

Query: 459 PQDYP 463
           P +YP
Sbjct: 424 PDEYP 428

[Close]

BLAST of CmaCh03G011030 vs. ExPASy Swiss-Prot
Match: P50136 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.7e-111
Identity = 196/414 (47.34%), Postives = 268/414 (64.73%), Query Frame = 0


Query: 51  AHRFKSTEVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQ 110
           A    S + ++Q  S++D        FPG   +F  +L F+       IP +RV+D  GQ
Sbjct: 30  ARSHPSRQQQQQFPSLDDKP-----QFPGASAEFVDKLEFIQPNVISGIPIYRVMDRQGQ 89

Query: 111 PSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAA 170
               S    + +E  +K Y  M  L TMD I YE+QR+GRISFY+T+ GEE  ++ SAAA
Sbjct: 90  IINPSEDPHLPQEEVLKFYRSMTLLNTMDRILYESQREGRISFYMTNYGEEGTHVGSAAA 149

Query: 171 LSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVAS 230
           L   DL+  QYREAGVL++R + L+ F SQC+GN  D  KGRQMPVHYG K  ++ T++S
Sbjct: 150 LERTDLVFGQYREAGVLMYRDYPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISS 209

Query: 231 TIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNN 290
            +A+QIP AVGAAY+ K    +   + YFG+G  SEGD HA  NFAA LE P+IF CRNN
Sbjct: 210 PLATQIPQAVGAAYAAKRANANRIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNN 269

Query: 291 GWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIE 350
           G+AISTP S+Q+R DG+  +G  YG++SIRVDGND  AVYN  + AR+ A+ E +P LIE
Sbjct: 270 GYAISTPTSEQYRGDGIAARGPGYGIKSIRVDGNDVFAVYNATKEARRRAVAENQPFLIE 329

Query: 351 ALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLR 410
           A+TYR+GHH+TSDDS+ YR V+EV +W     P+SR R+++ + GWW +E E   R   R
Sbjct: 330 AMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSR 389

Query: 411 KQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSD 465
           K+++EA + AER  KP  +  F+DVY   P+ L  Q+  L + ++T+ + YP D
Sbjct: 390 KKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQLRRQQESLARHLQTYGEHYPLD 438

[Close]

BLAST of CmaCh03G011030 vs. ExPASy Swiss-Prot
Match: A5A6H9 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes OX=9598 GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.7e-111
Identity = 197/405 (48.64%), Postives = 264/405 (65.19%), Query Frame = 0


Query: 60  EKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQ 119
           ++Q  S++D        FPG   +F  +L F+       IP +RV+D  GQ    S    
Sbjct: 42  QQQFSSLDDKP-----QFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPH 101

Query: 120 VSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVP 179
           + KE  +K+Y  M  L TMD I YE+QRQGRISFY+T+ GEE  ++ SAAAL   DL+  
Sbjct: 102 LPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFG 161

Query: 180 QYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHA 239
           QYREAGVL++R + L+ F +QC+GN  D  KGRQMPVHYG K  ++ T++S +A+QIP A
Sbjct: 162 QYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQA 221

Query: 240 VGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPIS 299
           VGAAY+ K    +   + YFG+G  SEGD HA  NFAA LE P+IF CRNNG+AISTP S
Sbjct: 222 VGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTS 281

Query: 300 DQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHH 359
           +Q+R DG+  +G  YG+ SIRVDGND  AVYN  + AR+ A+ E +P LIEA+TYR+GHH
Sbjct: 282 EQYRGDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHH 341

Query: 360 TTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQL 419
           +TSDDS+ YR V+EV +W     PVSR R ++ S GWW +E E   R   RK+++EA + 
Sbjct: 342 STSDDSSAYRSVDEVNYWDKQDHPVSRLRHYLLSQGWWDEEQEKAWRKQSRKKVMEAFEQ 401

Query: 420 AERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSD 465
           AER  KP     F+DVY   P+ L +Q+  L + ++T+ + YP D
Sbjct: 402 AERKPKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPLD 441

[Close]

BLAST of CmaCh03G011030 vs. ExPASy TrEMBL
Match: A0A6J1IRN8 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111478096 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 2.8e-274
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0


Query: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60
           MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE
Sbjct: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60

Query: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120
           KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV
Sbjct: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120

Query: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180
           SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ
Sbjct: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180

Query: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240
           YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV
Sbjct: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240

Query: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300
           GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD
Sbjct: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300

Query: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360
           QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT
Sbjct: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360

Query: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420
           TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA
Sbjct: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420

Query: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPLF 469
           ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPLF
Sbjct: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPLF 468

[Close]

BLAST of CmaCh03G011030 vs. ExPASy TrEMBL
Match: A0A6J1GE31 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111453112 PE=4 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.1e-267
Identity = 454/467 (97.22%), Postives = 462/467 (98.93%), Query Frame = 0


Query: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60
           MAFHVRKATKIIPHFRNGMR LGSLRE+PFAVHKIGNRVLGFENMLNHLS HRFKST+VE
Sbjct: 1   MAFHVRKATKIIPHFRNGMRFLGSLRETPFAVHKIGNRVLGFENMLNHLSTHRFKSTKVE 60

Query: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120
           KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEER+PCFRVLDDNGQPSMYSNFTQV
Sbjct: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLDDNGQPSMYSNFTQV 120

Query: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180
           SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTS+GEEAINIASAAALSLDDLIVPQ
Sbjct: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIASAAALSLDDLIVPQ 180

Query: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240
           YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV
Sbjct: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240

Query: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300
           GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD
Sbjct: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300

Query: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360
           QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTV+AARKMAIQERRPILIEALTYRVGHHT
Sbjct: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQERRPILIEALTYRVGHHT 360

Query: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420
           TSDDSTRYRPVEE+EWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLE IQLA
Sbjct: 361 TSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEEIQLA 420

Query: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
           ER+EKP VADAFTDVYDVPPSNL+EQERWLRKTIKTHPQDYPSDF L
Sbjct: 421 ERTEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFQL 467

[Close]

BLAST of CmaCh03G011030 vs. ExPASy TrEMBL
Match: A0A5A7SMQ8 (2-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00070 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.9e-240
Identity = 412/481 (85.65%), Postives = 438/481 (91.06%), Query Frame = 0


Query: 1   MAFHVRKA-TKIIPHFRNGMR-LLG------------SLRESPFAVHKIGNRVLGFENML 60
           MAF VRKA TKIIPHFRNGM   LG             LR + F+ HKIG R+LG ENM 
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  NHLSAHRFKSTEVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLD 120
            HL + RF+S +V KQ+D VNDDRDNQVL FPGGKVKFT QLSF+P+REEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIA 180
           DNGQPSMYSNFTQVSKE+AMK+Y+DMVTLQTMDTIFYEAQRQGRISFYVTS+GEEAIN+A
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300
           TVASTIASQIPHAVGAAYSMKM+K+DACAV YFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRP 360
           CRNNGWAISTP+SDQFRSDGVVVKG+AYGVRSIRVDGNDALAVYN V+AAR+MAIQE+RP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420
           ILIEALTYR GHHTTSDDSTRYRP EE+EWWRVA DPVSRFRKWIESNGWWS+EAE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SSLRKQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFP 468
           SSLRKQLLE IQLAER EKP VADAFTDVYDVPPSNL EQE+WLRKTIKTHPQDYPS+FP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

[Close]

BLAST of CmaCh03G011030 vs. ExPASy TrEMBL
Match: A0A1S3B380 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103485287 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.9e-240
Identity = 412/481 (85.65%), Postives = 438/481 (91.06%), Query Frame = 0


Query: 1   MAFHVRKA-TKIIPHFRNGMR-LLG------------SLRESPFAVHKIGNRVLGFENML 60
           MAF VRKA TKIIPHFRNGM   LG             LR + F+ HKIG R+LG ENM 
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  NHLSAHRFKSTEVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLD 120
            HL + RF+S +V KQ+D VNDDRDNQVL FPGGKVKFT QLSF+P+REEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIA 180
           DNGQPSMYSNFTQVSKE+AMK+Y+DMVTLQTMDTIFYEAQRQGRISFYVTS+GEEAIN+A
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300
           TVASTIASQIPHAVGAAYSMKM+K+DACAV YFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRP 360
           CRNNGWAISTP+SDQFRSDGVVVKG+AYGVRSIRVDGNDALAVYN V+AAR+MAIQE+RP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420
           ILIEALTYR GHHTTSDDSTRYRP EE+EWWRVA DPVSRFRKWIESNGWWS+EAE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SSLRKQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFP 468
           SSLRKQLLE IQLAER EKP VADAFTDVYDVPPSNL EQE+WLRKTIKTHPQDYPS+FP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

[Close]

BLAST of CmaCh03G011030 vs. ExPASy TrEMBL
Match: A0A6J1BTD3 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005384 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.4e-238
Identity = 409/481 (85.03%), Postives = 441/481 (91.68%), Query Frame = 0


Query: 1   MAFHVRKATKIIPHFRNG-MRLLGS-------------LRESPFAVHKIGNRVLGFENML 60
           MAF  RKATKIIPHF N  M  LGS             L ++PF+V KIGN V+GFE+M 
Sbjct: 1   MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP 60

Query: 61  NHLSAHRFKSTEVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLD 120
            HLSA RFKS +VEK++DSVNDDRDNQVL FPGGKV+FTP +SF+P+R++ERIPCFRVLD
Sbjct: 61  THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIA 180
           DNGQ S++SNFTQVSKE+A+KMYNDMVTLQTMDTI YEAQRQGRISFY+TSIGEEAINIA
Sbjct: 121 DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA 180

Query: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300
           TVASTIASQIPHAVGAAYSMKMEK+DACAVTYFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRP 360
           CRNNGWAISTPISDQFRSDGV+VKGRAYGVRSIRVDGNDALAVYN V+AAR+MAIQE+RP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420
           ILIEALTYRVGHHTTSDDSTRYRP EE+EWWRVAKDPVSRFRKWIESNGWWS+EAE+ELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR 420

Query: 421 SSLRKQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFP 468
           SSLRKQLLE IQLAER EKP VA+AFTDVYDVPPSNL EQE WLRKTIKTHPQDYPS+FP
Sbjct: 421 SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP 480

[Close]

BLAST of CmaCh03G011030 vs. NCBI nr
Match: XP_022977963.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 953.7 bits (2464), Expect = 5.7e-274
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0


Query: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60
           MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE
Sbjct: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60

Query: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120
           KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV
Sbjct: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120

Query: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180
           SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ
Sbjct: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180

Query: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240
           YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV
Sbjct: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240

Query: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300
           GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD
Sbjct: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300

Query: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360
           QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT
Sbjct: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360

Query: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420
           TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA
Sbjct: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420

Query: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPLF 469
           ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPLF
Sbjct: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPLF 468

[Close]

BLAST of CmaCh03G011030 vs. NCBI nr
Match: XP_023543630.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 933.3 bits (2411), Expect = 8.0e-268
Identity = 454/467 (97.22%), Postives = 464/467 (99.36%), Query Frame = 0


Query: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60
           MAFHVRKATKIIPHFRNGMR LGSLRE+PFAVHKIGN+VLGFENMLNHLSAHRFKST+VE
Sbjct: 1   MAFHVRKATKIIPHFRNGMRFLGSLRETPFAVHKIGNKVLGFENMLNHLSAHRFKSTKVE 60

Query: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120
           KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEER+PCFRVLDDNGQPSM+SNFTQV
Sbjct: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLDDNGQPSMFSNFTQV 120

Query: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180
           SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTS+GEEAINIASAAALSLDDLIVPQ
Sbjct: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIASAAALSLDDLIVPQ 180

Query: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240
           YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV
Sbjct: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240

Query: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300
           GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD
Sbjct: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300

Query: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360
           QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTV+AARKMAIQE+RPILIEALTYRVGHHT
Sbjct: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQEQRPILIEALTYRVGHHT 360

Query: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420
           TSDDSTRYRPVEE+EWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLE IQLA
Sbjct: 361 TSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEEIQLA 420

Query: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
           ERSEKP VADAFTDVYDVPPSNL+EQERWLRKTIKTHPQDYPSDFPL
Sbjct: 421 ERSEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFPL 467

[Close]

BLAST of CmaCh03G011030 vs. NCBI nr
Match: KAG7034451.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 932.9 bits (2410), Expect = 1.0e-267
Identity = 455/467 (97.43%), Postives = 462/467 (98.93%), Query Frame = 0


Query: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60
           MAFHVRKATKIIPHFRNGMR LGSLRE+PFAVHKIGNRVLGFENMLNHLS HRFKST+VE
Sbjct: 1   MAFHVRKATKIIPHFRNGMRFLGSLRETPFAVHKIGNRVLGFENMLNHLSTHRFKSTKVE 60

Query: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120
           KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEER+PCFRVLDDNGQPSMYSNFTQV
Sbjct: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLDDNGQPSMYSNFTQV 120

Query: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180
           SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTS+GEEAINIASAAALSLDDLIVPQ
Sbjct: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIASAAALSLDDLIVPQ 180

Query: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240
           YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV
Sbjct: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240

Query: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300
           GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD
Sbjct: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300

Query: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360
           QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTV+AARKMAIQERRPILIEALTYRVGHHT
Sbjct: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQERRPILIEALTYRVGHHT 360

Query: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420
           TSDDSTRYRPVEE+EWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLE IQLA
Sbjct: 361 TSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEEIQLA 420

Query: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
           ERSEKP VADAFTDVYDVPPSNL+EQERWLRKTIKTHPQDYPSDF L
Sbjct: 421 ERSEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFQL 467

[Close]

BLAST of CmaCh03G011030 vs. NCBI nr
Match: XP_022949834.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 931.8 bits (2407), Expect = 2.3e-267
Identity = 454/467 (97.22%), Postives = 462/467 (98.93%), Query Frame = 0


Query: 1   MAFHVRKATKIIPHFRNGMRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVE 60
           MAFHVRKATKIIPHFRNGMR LGSLRE+PFAVHKIGNRVLGFENMLNHLS HRFKST+VE
Sbjct: 1   MAFHVRKATKIIPHFRNGMRFLGSLRETPFAVHKIGNRVLGFENMLNHLSTHRFKSTKVE 60

Query: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQV 120
           KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEER+PCFRVLDDNGQPSMYSNFTQV
Sbjct: 61  KQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLDDNGQPSMYSNFTQV 120

Query: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQ 180
           SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTS+GEEAINIASAAALSLDDLIVPQ
Sbjct: 121 SKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIASAAALSLDDLIVPQ 180

Query: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240
           YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV
Sbjct: 181 YREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAV 240

Query: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300
           GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD
Sbjct: 241 GAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISD 300

Query: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHT 360
           QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTV+AARKMAIQERRPILIEALTYRVGHHT
Sbjct: 301 QFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQERRPILIEALTYRVGHHT 360

Query: 361 TSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLA 420
           TSDDSTRYRPVEE+EWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLE IQLA
Sbjct: 361 TSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEEIQLA 420

Query: 421 ERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
           ER+EKP VADAFTDVYDVPPSNL+EQERWLRKTIKTHPQDYPSDF L
Sbjct: 421 ERTEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFQL 467

[Close]

BLAST of CmaCh03G011030 vs. NCBI nr
Match: KAG6604290.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 899.4 bits (2323), Expect = 1.3e-257
Identity = 438/449 (97.55%), Postives = 446/449 (99.33%), Query Frame = 0


Query: 19  MRLLGSLRESPFAVHKIGNRVLGFENMLNHLSAHRFKSTEVEKQIDSVNDDRDNQVLNFP 78
           MR LGSLRE+PFAVHKIGNRVLGFENMLNHLSAHRFKST+VEKQIDSVNDDRDNQVLNFP
Sbjct: 1   MRFLGSLRETPFAVHKIGNRVLGFENMLNHLSAHRFKSTKVEKQIDSVNDDRDNQVLNFP 60

Query: 79  GGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTM 138
           GGKVKFTPQLSF+PEREEER+PCFRVLDDNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTM
Sbjct: 61  GGKVKFTPQLSFIPEREEERVPCFRVLDDNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTM 120

Query: 139 DTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFA 198
           DTIFYEAQRQGRISFYVTS+GEEAINIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFA
Sbjct: 121 DTIFYEAQRQGRISFYVTSLGEEAINIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFA 180

Query: 199 SQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTY 258
           SQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTY
Sbjct: 181 SQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTY 240

Query: 259 FGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISDQFRSDGVVVKGRAYGVRS 318
           FGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISDQFRSDGVVVKGRAYGVRS
Sbjct: 241 FGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISDQFRSDGVVVKGRAYGVRS 300

Query: 319 IRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHTTSDDSTRYRPVEEVEWWR 378
           IRVDGNDALAVYNTV+AARKMAIQERRPILIEALTYRVGHHTTSDDSTRYRPVEE+EWWR
Sbjct: 301 IRVDGNDALAVYNTVRAARKMAIQERRPILIEALTYRVGHHTTSDDSTRYRPVEEMEWWR 360

Query: 379 VAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLAERSEKPTVADAFTDVYDV 438
           VAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLE IQLAERSEKP VADAFTDVYDV
Sbjct: 361 VAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEEIQLAERSEKPPVADAFTDVYDV 420

Query: 439 PPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
           PPSNL+EQERWLRKTIKTHPQDYPSDFPL
Sbjct: 421 PPSNLEEQERWLRKTIKTHPQDYPSDFPL 449

[Close]

BLAST of CmaCh03G011030 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 629.8 bits (1623), Expect = 1.8e-180
Identity = 291/397 (73.30%), Postives = 354/397 (89.17%), Query Frame = 0


Query: 71  DNQVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQVSKEIAMKMYN 130
           ++QV++FPGGKV FTP++ F+ E ++ER+PC+RVLDDNGQ    S F QVS+E+A+K+Y+
Sbjct: 76  NHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYS 135

Query: 131 DMVTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQYREAGVLVWR 190
           DMVTLQ MD IFYEAQRQGR+SFY T+IGEEAINIASAAAL+  D+I PQYRE GVL+WR
Sbjct: 136 DMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWR 195

Query: 191 GFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMEK 250
           GFTL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KM+K
Sbjct: 196 GFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDK 255

Query: 251 RDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISDQFRSDGVVVK 310
           +DACAVTYFGDGGTSEGDFHAALN AAV+E PV+FICRNNGWAISTP SDQFRSDGVVVK
Sbjct: 256 KDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVK 315

Query: 311 GRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHTTSDDSTRYRP 370
           GRAYG+RSIRVDGNDALA+Y+ V  AR+MAI+E+RPILIEALTYRVGHH+TSDDSTRYR 
Sbjct: 316 GRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRS 375

Query: 371 VEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLAERSEKPTVAD 430
             E+EWW  A++P+SRFR WIESNGWWSD+ E++LRS ++K++LEA+++AE++EKP + +
Sbjct: 376 AGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQN 435

Query: 431 AFTDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
            F+DVYDVPPSNL EQE  +R+TI +HPQDYPSD PL
Sbjct: 436 MFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

[Close]

BLAST of CmaCh03G011030 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 629.4 bits (1622), Expect = 2.3e-180
Identity = 291/395 (73.67%), Postives = 352/395 (89.11%), Query Frame = 0


Query: 73  QVLNFPGGKVKFTPQLSFVPEREEERIPCFRVLDDNGQPSMYSNFTQVSKEIAMKMYNDM 132
           QV++FPGGKV FTP++ F+ E ++ER+PC+RVLDDNGQ    S F QVS+E+A+K+Y+DM
Sbjct: 7   QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 66

Query: 133 VTLQTMDTIFYEAQRQGRISFYVTSIGEEAINIASAAALSLDDLIVPQYREAGVLVWRGF 192
           VTLQ MD IFYEAQRQGR+SFY T+IGEEAINIASAAAL+  D+I PQYRE GVL+WRGF
Sbjct: 67  VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 126

Query: 193 TLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMEKRD 252
           TL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KM+K+D
Sbjct: 127 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKD 186

Query: 253 ACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPISDQFRSDGVVVKGR 312
           ACAVTYFGDGGTSEGDFHAALN AAV+E PV+FICRNNGWAISTP SDQFRSDGVVVKGR
Sbjct: 187 ACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGR 246

Query: 313 AYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHHTTSDDSTRYRPVE 372
           AYG+RSIRVDGNDALA+Y+ V  AR+MAI+E+RPILIEALTYRVGHH+TSDDSTRYR   
Sbjct: 247 AYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAG 306

Query: 373 EVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQLAERSEKPTVADAF 432
           E+EWW  A++P+SRFR WIESNGWWSD+ E++LRS ++K++LEA+++AE++EKP + + F
Sbjct: 307 EIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMF 366

Query: 433 TDVYDVPPSNLDEQERWLRKTIKTHPQDYPSDFPL 468
           +DVYDVPPSNL EQE  +R+TI +HPQDYPSD PL
Sbjct: 367 SDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 401

[Close]

BLAST of CmaCh03G011030 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 584.7 bits (1506), Expect = 6.5e-167
Identity = 272/423 (64.30%), Postives = 349/423 (82.51%), Query Frame = 0


Query: 46  LNHLSAHRFKSTEVEKQIDSV---NDDRDNQVLNFPGGKVKFTPQLSFVPEREEERIPCF 105
           L+   + R +ST VE Q D +    D+ D Q L+FPGGKV +T ++ F+PE    RIPC+
Sbjct: 48  LSPFGSLRHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCY 107

Query: 106 RVLDDNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSIGEEA 165
           RVLD++G+    S+F  VS+++A++MY  M TLQ MD IFYEAQRQGRISFY+TS+GEEA
Sbjct: 108 RVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEA 167

Query: 166 INIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKR 225
           INIASAAALS DD+++PQYRE GVL+WRGFTL+EFA+QCFGNK DY KGRQMP+HYGS R
Sbjct: 168 INIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNR 227

Query: 226 HNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVP 285
            NYFT++S IA+Q+P A G  YS+KM+K++AC VT+ GDGGTSEGDFHA LNFAAV+E P
Sbjct: 228 LNYFTISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAP 287

Query: 286 VIFICRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQ 345
           V+FICRNNGWAIST IS+QFRSDG+VVKG+AYG+RSIRVDGNDALAVY+ V++AR+MA+ 
Sbjct: 288 VVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVT 347

Query: 346 ERRPILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAE 405
           E+RP+LIE +TYRVGHH+TSDDST+YR  +E+++W+++++PV+RFRKW+E NGWWS+E E
Sbjct: 348 EQRPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDE 407

Query: 406 AELRSSLRKQLLEAIQLAERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDYP 465
           ++LRS+ RKQLL+AIQ AE+ EK  + + F DVYDV P NL+EQE  L++ +K  PQDYP
Sbjct: 408 SKLRSNARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYP 467

[Close]

BLAST of CmaCh03G011030 vs. TAIR 10
Match: AT5G34780.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 308.5 bits (789), Expect = 9.0e-84
Identity = 140/222 (63.06%), Postives = 186/222 (83.78%), Query Frame = 0


Query: 240 VGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFICRNNGWAISTPIS 299
           +G + +    +++ACAVT+ GDGGTSEGDFHA LNFAAV+E PV+FICRNNGWAIST IS
Sbjct: 15  IGLSKAKDCWEKNACAVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHIS 74

Query: 300 DQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARKMAIQERRPILIEALTYRVGHH 359
           +QFRSDG+VVKG+AYG+RSIRVDGNDALAVY+ V +AR+MA+ E+RP+LIE + YRVGHH
Sbjct: 75  EQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHH 134

Query: 360 TTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWSDEAEAELRSSLRKQLLEAIQL 419
           +TSDDST+YR  +E+++W+++++ V+RFRK +E NGWWS+E E++LRS+ RKQLL+AIQ 
Sbjct: 135 STSDDSTKYRAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQA 194

Query: 420 AERSEKPTVADAFTDVYDVPPSNLDEQERWLRKTIKTHPQDY 462
           AE+ EK  + + F DVYDV P NL+E+E  L++ I+  PQDY
Sbjct: 195 AEKWEKQPLTELFNDVYDVKPKNLEEEELGLKELIEKQPQDY 236

[Close]

BLAST of CmaCh03G011030 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 149.1 bits (375), Expect = 9.1e-36
Identity = 82/278 (29.50%), Postives = 148/278 (53.24%), Query Frame = 0


Query: 159 GEEAINIASAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHY 218
           G+EA+ I   AA++  D I+  YR+  + + RG +L E  S+  G +    KG+   +H+
Sbjct: 94  GQEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHF 153

Query: 219 GSKRHNYFTVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAV 278
             K  +++     + +Q+P   G A++ K  K +A     +GDG  ++G    ALN +A+
Sbjct: 154 YKKESSFYGGHGIVGAQVPLGCGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISAL 213

Query: 279 LEVPVIFICRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVQAARK 338
            ++P I +C NN + + T      +S     +G    V  ++VDG DA AV    + A++
Sbjct: 214 WDLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQ 273

Query: 339 MAIQERRPILIEALTYRVGHHTTSDDSTRYRPVEEVEWWRVAKDPVSRFRKWIESNGWWS 398
            A+ E+ PI++E  TYR   H+ SD  + YR  +E+   R  +DP+ R +K + S+   +
Sbjct: 274 HAL-EKGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLAT 333

Query: 399 DEAEAELRSSLRKQLLEAIQLAERSEKPTVADAFTDVY 437
           ++   ++   +RK++ +AI  A+    P  ++ FT+VY
Sbjct: 334 EKELKDMEKEIRKEVDDAIAKAKDCPMPEPSELFTNVY 368

The following BLAST results are available for this feature:

BLAST of CmaCh03G011030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84JL2	2.5e-179	73.30	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis tha...	[more]
Q9LPL5	9.2e-166	64.30	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis tha...	[more]
Q54M22	9.0e-113	51.51	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium dis...	[more]
P50136	1.7e-111	47.34	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=1...	[more]
A5A6H9	1.7e-111	48.64	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes O...	[more]

BLAST of CmaCh03G011030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IRN8	2.8e-274	100.00	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita maxim...	[more]
A0A6J1GE31	1.1e-267	97.22	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita mosch...	[more]
A0A5A7SMQ8	9.9e-240	85.65	2-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3B380	9.9e-240	85.65	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX...	[more]
A0A6J1BTD3	1.4e-238	85.03	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica chara...	[more]

BLAST of CmaCh03G011030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022977963.1	5.7e-274	100.00	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita maxima]	[more]
XP_023543630.1	8.0e-268	97.22	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo su...	[more]
KAG7034451.1	1.0e-267	97.43	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita argyros...	[more]
XP_022949834.1	2.3e-267	97.22	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita moschat...	[more]
KAG6604290.1	1.3e-257	97.55	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial, partial [Cucurbit...	[more]

BLAST of CmaCh03G011030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09300.1	1.8e-180	73.30	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.2	2.3e-180	73.67	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT1G21400.1	6.5e-167	64.30	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G34780.1	9.0e-84	63.06	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT1G59900.1	9.1e-36	29.50	pyruvate dehydrogenase complex E1 alpha subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 132..428 e-value: 3.2E-93 score: 312.0
None	No IPR available	GENE3D	3.40.50.970		coord: 70..465 e-value: 1.7E-144 score: 483.6
None	No IPR available	PANTHER	PTHR43380:SF11	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL	coord: 50..467
None	No IPR available	PANTHER	PTHR43380	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL	coord: 50..467
None	No IPR available	CDD	cd02000	TPP_E1_PDC_ADC_BCADC	coord: 128..420 e-value: 2.43751E-149 score: 425.371
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 101..463

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G011030.1	CmaCh03G011030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009083	branched-chain amino acid catabolic process
cellular_component	GO:0005947	mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor