CmaCh03G008220 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G008220
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCma_Chr03: 6253749 .. 6254894 (+)
RNA-Seq ExpressionCmaCh03G008220
SyntenyCmaCh03G008220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCTGCCACCGGGCTTGTCTTGGGTCCCGATATCATTTTAAATTTTGCTATACACTCTGTACTCCTAACTGTTCCCTTCTATGTTCTCCAAAGTCACTTCCGCCGCATCTGCCTTTGCTCCCGTCTTCCGGTAACCCACCCCGCCCCCATCCCTCGGGAAAGACTCTACTTCTTCTCAGTTTGATCTCCACAGCTACTTTCATCGTCGCCATCTTCATGGTTTGTCTTCTTGTCAAGAAATTGAAGGAGTATCGTAACTCCAGACAGAATGGGCCGCCTCTGTTCGATGTCCACAGAGAGAACCTCCCCGATGAAGATGAAGAAGCACCCGTCCTTGACCACCACGTATGGTACATCAATACCGTCGGTCTCCAGCAATCGATCATCGATTCAATCACCATGTTCAAGTACAGGAAGGACGAGAAATTAATCGAAGGATCAGACTGCTCGATCTGCCTCGGCGAGTTTCAAGACGACGAGAGCCTTCGACTGTTGCCTAAATGCAGCCACGCCTTCCATGTTCCCTGCATCGACACCTGGCTGAGATCGCACAAAAACTGCCCCCTTTGTCGTGCTCCAGTGCTGTCCAACTCCGCCGCCCCTAATTTAGTCCCCACCGAACCGATCAGAAACCAGCCGCCGGCGATCGAAGTTACCCAAATGGAAACAGAGGCCGTCGAGGAAGAAAACAGAGGAGATGCAAACAGTGGAGAAGGGGGTAGCAATAGTGGAGGGAATTCTGAAATTCCAATCAAAGGGCTGTCTCTGACTTTGAGTGATTTGACTGAGAATCAAATTCGCGAGGCTGTGGAAGACGAAGGACAAGCGATAAGAAGGTCGGTTTCTATGGATTCAGTTTCTGCAATGGCGATTTATCAGGCGACGGCCAAAATACAAATTCCAGAAGAAGGTTCTTCTTCAAGTGGGGCGTTAGTGGAAGTGAAGAATTCAGTCTCTTCGAGGAATGTTCCCTTCCCAAAGCGGGGAGTCGGAAATCAGAGCTTGTATAATCTCGTGAAGAGCATTCCATTTGGGCGTTCTGTGCAAAAGGGGGGGATCTCGATGAAAATGTTAAGTTCGGGTAGAAGGAAGATACATTCGGCCAGGCATTACAGAAGCCAGAGCTCGGTCCTTCCGATGTGA

mRNA sequence

ATGCCCTGCCACCGGGCTTGTCTTGGGTCCCGATATCATTTTAAATTTTGCTATACACTCTGTACTCCTAACTGTTCCCTTCTATGTTCTCCAAAGTCACTTCCGCCGCATCTGCCTTTGCTCCCGTCTTCCGGTAACCCACCCCGCCCCCATCCCTCGGGAAAGACTCTACTTCTTCTCAGTTTGATCTCCACAGCTACTTTCATCGTCGCCATCTTCATGGTTTGTCTTCTTGTCAAGAAATTGAAGGAGTATCGTAACTCCAGACAGAATGGGCCGCCTCTGTTCGATGTCCACAGAGAGAACCTCCCCGATGAAGATGAAGAAGCACCCGTCCTTGACCACCACGTATGGTACATCAATACCGTCGGTCTCCAGCAATCGATCATCGATTCAATCACCATGTTCAAGTACAGGAAGGACGAGAAATTAATCGAAGGATCAGACTGCTCGATCTGCCTCGGCGAGTTTCAAGACGACGAGAGCCTTCGACTGTTGCCTAAATGCAGCCACGCCTTCCATGTTCCCTGCATCGACACCTGGCTGAGATCGCACAAAAACTGCCCCCTTTGTCGTGCTCCAGTGCTGTCCAACTCCGCCGCCCCTAATTTAGTCCCCACCGAACCGATCAGAAACCAGCCGCCGGCGATCGAAGTTACCCAAATGGAAACAGAGGCCGTCGAGGAAGAAAACAGAGGAGATGCAAACAGTGGAGAAGGGGGTAGCAATAGTGGAGGGAATTCTGAAATTCCAATCAAAGGGCTGTCTCTGACTTTGAGTGATTTGACTGAGAATCAAATTCGCGAGGCTGTGGAAGACGAAGGACAAGCGATAAGAAGGTCGGTTTCTATGGATTCAGTTTCTGCAATGGCGATTTATCAGGCGACGGCCAAAATACAAATTCCAGAAGAAGGTTCTTCTTCAAGTGGGGCGTTAGTGGAAGTGAAGAATTCAGTCTCTTCGAGGAATGTTCCCTTCCCAAAGCGGGGAGTCGGAAATCAGAGCTTGTATAATCTCGTGAAGAGCATTCCATTTGGGCGTTCTGTGCAAAAGGGGGGGATCTCGATGAAAATGTTAAGTTCGGGTAGAAGGAAGATACATTCGGCCAGGCATTACAGAAGCCAGAGCTCGGTCCTTCCGATGTGA

Coding sequence (CDS)

ATGCCCTGCCACCGGGCTTGTCTTGGGTCCCGATATCATTTTAAATTTTGCTATACACTCTGTACTCCTAACTGTTCCCTTCTATGTTCTCCAAAGTCACTTCCGCCGCATCTGCCTTTGCTCCCGTCTTCCGGTAACCCACCCCGCCCCCATCCCTCGGGAAAGACTCTACTTCTTCTCAGTTTGATCTCCACAGCTACTTTCATCGTCGCCATCTTCATGGTTTGTCTTCTTGTCAAGAAATTGAAGGAGTATCGTAACTCCAGACAGAATGGGCCGCCTCTGTTCGATGTCCACAGAGAGAACCTCCCCGATGAAGATGAAGAAGCACCCGTCCTTGACCACCACGTATGGTACATCAATACCGTCGGTCTCCAGCAATCGATCATCGATTCAATCACCATGTTCAAGTACAGGAAGGACGAGAAATTAATCGAAGGATCAGACTGCTCGATCTGCCTCGGCGAGTTTCAAGACGACGAGAGCCTTCGACTGTTGCCTAAATGCAGCCACGCCTTCCATGTTCCCTGCATCGACACCTGGCTGAGATCGCACAAAAACTGCCCCCTTTGTCGTGCTCCAGTGCTGTCCAACTCCGCCGCCCCTAATTTAGTCCCCACCGAACCGATCAGAAACCAGCCGCCGGCGATCGAAGTTACCCAAATGGAAACAGAGGCCGTCGAGGAAGAAAACAGAGGAGATGCAAACAGTGGAGAAGGGGGTAGCAATAGTGGAGGGAATTCTGAAATTCCAATCAAAGGGCTGTCTCTGACTTTGAGTGATTTGACTGAGAATCAAATTCGCGAGGCTGTGGAAGACGAAGGACAAGCGATAAGAAGGTCGGTTTCTATGGATTCAGTTTCTGCAATGGCGATTTATCAGGCGACGGCCAAAATACAAATTCCAGAAGAAGGTTCTTCTTCAAGTGGGGCGTTAGTGGAAGTGAAGAATTCAGTCTCTTCGAGGAATGTTCCCTTCCCAAAGCGGGGAGTCGGAAATCAGAGCTTGTATAATCTCGTGAAGAGCATTCCATTTGGGCGTTCTGTGCAAAAGGGGGGGATCTCGATGAAAATGTTAAGTTCGGGTAGAAGGAAGATACATTCGGCCAGGCATTACAGAAGCCAGAGCTCGGTCCTTCCGATGTGA

Protein sequence

MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLLSLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEGGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLSSGRRKIHSARHYRSQSSVLPM
Homology
BLAST of CmaCh03G008220 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.6e-35
Identity = 78/159 (49.06%), Postives = 104/159 (65.41%), Query Frame = 0

Query: 47  PPRPHPSGKTLLL-LSLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPD 106
           PP P P   +LLL  S+    + I+ +F+V  L   + + R          D +++   D
Sbjct: 30  PPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQEDED 89

Query: 107 EDEEAPVLD-HHVWYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLR 166
           E EE    D HHVW I TVGL +S I+SIT+  ++K E +I+G++CS+CL EF++DESLR
Sbjct: 90  ETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEEDESLR 149

Query: 167 LLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSNSAAPN 204
           LLPKCSHAFH+ CIDTWL SHKNCPLCRAPVL  +  P+
Sbjct: 150 LLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPH 188

BLAST of CmaCh03G008220 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 6.3e-32
Identity = 99/261 (37.93%), Postives = 138/261 (52.87%), Query Frame = 0

Query: 20  LCTPNCSLLCSPK----SLPPHLPLLPSSGNP-PRPHPSGKTLLL--------LSLISTA 79
           +C P C   C P+    ++ P LP   SS  P P P PS   + L        L  IS  
Sbjct: 27  VCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISII 86

Query: 80  TFIVAIFMVCL----LVKKLKEYRNSRQNGPPLFDVHREN---LPDEDEEAPVLDHHVWY 139
           T   A+  + L    LV K      +R N       + +N   + +E ++   +DH +W 
Sbjct: 87  TITGAVLAILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQVDHPIWL 146

Query: 140 INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID 199
           I T GLQQSII+SIT+  Y++ + LIE +DC +CL EF++DESLRLLPKC+HAFH+ CID
Sbjct: 147 IRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFHISCID 206

Query: 200 TWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVT------QMETEAVEEENRG 254
           TWL SH NCPLCRA +   S       T P  + P  +EVT       +E + V+EE+ G
Sbjct: 207 TWLSSHTNCPLCRAGIAMISV------TTPRYSGP--VEVTPGGSGSHLENDGVDEEDHG 266

BLAST of CmaCh03G008220 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 2.8e-27
Identity = 67/163 (41.10%), Postives = 94/163 (57.67%), Query Frame = 0

Query: 124 GLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDTWLR 183
           GL +S+I SIT++KYRK +  +E SDCS+CL EFQ++ESLRLLPKC+HAFHVPCIDTWL+
Sbjct: 133 GLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLK 192

Query: 184 SHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEGGSN 243
           SH NCPLCRA ++++S                A+E+  +  + +  EN          S 
Sbjct: 193 SHSNCPLCRAFIVTSS----------------AVEIVDLTNQQIVTEN---------NSI 252

Query: 244 SGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDS 287
           S G+  + +  L     DL  ++ R    +EG   +     DS
Sbjct: 253 STGDDSVVVVNL-----DLENSRSRNETVNEGSTPKPPEMQDS 265

BLAST of CmaCh03G008220 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.3e-26
Identity = 79/202 (39.11%), Postives = 114/202 (56.44%), Query Frame = 0

Query: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180
           N  GL +S+I SIT++KY+  +  ++GSDCS+CL EF+++ESLRLLPKC+HAFH+PCIDT
Sbjct: 113 NGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDT 172

Query: 181 WLRSHKNCPLCRA---PVLSNSAAPNLVPTEPIRNQPPAIEVTQMETE-----AVEEENR 240
           WL+SH NCPLCRA    V + +A+     +  + NQ  +   T   +E     A  E   
Sbjct: 173 WLKSHSNCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQT 232

Query: 241 GDANS-------GEGGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMD 300
           GD +S         G  NS   SE+ +             + RE  +++   IRRSVS++
Sbjct: 233 GDFDSVVVIEDLEIGSRNSDARSELQL-----------PEERRETKDEDSLPIRRSVSLN 292

Query: 301 SVSAMAIYQATAKIQIPEEGSS 308
           S   ++I     +I+  EEG S
Sbjct: 293 SGVVVSIADVLREIE-DEEGES 302

BLAST of CmaCh03G008220 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 6.4e-24
Identity = 47/82 (57.32%), Postives = 62/82 (75.61%), Query Frame = 0

Query: 115 HHVWYIN-TVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAF 174
           H  W ++   GL +++I+ IT+ KYR+ +  +  +DCS+CLGEF D ESLRLLP+CSHAF
Sbjct: 121 HESWNVSPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAF 180

Query: 175 HVPCIDTWLRSHKNCPLCRAPV 196
           H  CIDTWL+SH NCPLCRA +
Sbjct: 181 HQQCIDTWLKSHSNCPLCRANI 202

BLAST of CmaCh03G008220 vs. ExPASy TrEMBL
Match: A0A6J1IL98 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478464 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 4.0e-215
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60
           MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL
Sbjct: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60

Query: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI 120
           SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI
Sbjct: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI 120

Query: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180
           NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT
Sbjct: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180

Query: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG 240
           WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG
Sbjct: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG 240

Query: 241 GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300
           GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ
Sbjct: 241 GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300

Query: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360
           IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS
Sbjct: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360

Query: 361 SGRRKIHSARHYRSQSSVLPM 382
           SGRRKIHSARHYRSQSSVLPM
Sbjct: 361 SGRRKIHSARHYRSQSSVLPM 381

BLAST of CmaCh03G008220 vs. ExPASy TrEMBL
Match: A0A6J1GCY1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453059 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.2e-206
Identity = 366/381 (96.06%), Postives = 371/381 (97.38%), Query Frame = 0

Query: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60
           MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPK  PPHLPLLPSSGNPPRPHPSGKTLLLL
Sbjct: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKPPPPHLPLLPSSGNPPRPHPSGKTLLLL 60

Query: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI 120
           SLISTAT +VAIFM+CLLVKKLKEYRNSRQNGPPLFDVHRENLPD+DEE PVLDHHVWYI
Sbjct: 61  SLISTATLVVAIFMLCLLVKKLKEYRNSRQNGPPLFDVHRENLPDDDEEGPVLDHHVWYI 120

Query: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180
           NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT
Sbjct: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180

Query: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG 240
           WLRSHKNCPLCRAPVLSNSAAPNLVP EPI NQPPAIE TQMETEAVEEENRG+ NSGEG
Sbjct: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPIEPIGNQPPAIEDTQMETEAVEEENRGNENSGEG 240

Query: 241 GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300
           GSNSGGNSEIPIKGLS TLSDLTENQ+REAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ
Sbjct: 241 GSNSGGNSEIPIKGLSRTLSDLTENQVREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300

Query: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360
           IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS
Sbjct: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360

Query: 361 SGRRKIHSARHYRSQSSVLPM 382
           SGRRKIH ARHYRSQSSVLPM
Sbjct: 361 SGRRKIHWARHYRSQSSVLPM 381

BLAST of CmaCh03G008220 vs. ExPASy TrEMBL
Match: A0A0A0KK07 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G499220 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.9e-151
Identity = 295/397 (74.31%), Postives = 317/397 (79.85%), Query Frame = 0

Query: 1   MPCHRACLG-SRYHFKFCYTLCTPNCSLLC----------SPKSLPP---HLPLLPSSGN 60
           MPC   C   +  HF+ C  LC+P CS +C           P+  PP    LP LPSS  
Sbjct: 1   MPCQSNCAPITPSHFQVCTLLCSPKCSSICFQLPKAPIVDLPRPPPPPPLPLPQLPSSIV 60

Query: 61  PPRPH-PSGKTLLLLSLISTATFIVAIFMVCLLVKKLKEYRNSRQNG-PPLFDVHRENLP 120
           PP+ H P   TLLLLSLI+TAT + AIF++CLL KK +EYRNSR N  P LFDV  ENLP
Sbjct: 61  PPQNHTPERSTLLLLSLITTATLVAAIFLLCLLFKKFREYRNSRHNRLPVLFDVQAENLP 120

Query: 121 DEDEEAPVLDHHVWYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLR 180
           D DEE PV+DHHVWYINTVGLQQS IDSITMFKYRKDEKLI+GSDCSICLGEFQDDESLR
Sbjct: 121 DNDEEEPVIDHHVWYINTVGLQQSAIDSITMFKYRKDEKLIDGSDCSICLGEFQDDESLR 180

Query: 181 LLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMET 240
           LLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLS+ A P+L P EPI NQP  IE  Q ET
Sbjct: 181 LLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSDPATPSLAPIEPITNQPAPIENIQRET 240

Query: 241 EAVEEENRGDANSGEGGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSM 300
           EA+EEENR D NSGE GSNSG NS IPIK LS TLS+LTENQ REAVEDEGQ IRRSVSM
Sbjct: 241 EAIEEENREDENSGEVGSNSGENSVIPIKRLSRTLSNLTENQNREAVEDEGQGIRRSVSM 300

Query: 301 DSVSAMAIYQATAKIQIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIP 360
           DSVSAMAIYQATAKIQIPEEG SSSG L+EVKNSVSSRN+PFPK G+GNQSLYNLVK I 
Sbjct: 301 DSVSAMAIYQATAKIQIPEEGCSSSGTLMEVKNSVSSRNLPFPKGGIGNQSLYNLVKGIS 360

Query: 361 FGRSVQKGGISMKMLSSGRRKIHSARHYRSQSSVLPM 382
           FG S+QKGGISMK  SSGRRK+  ARHYRSQSSVLPM
Sbjct: 361 FGCSMQKGGISMKRFSSGRRKLQWARHYRSQSSVLPM 397

BLAST of CmaCh03G008220 vs. ExPASy TrEMBL
Match: A0A5A7T0B5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003050 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.3e-136
Identity = 279/402 (69.40%), Postives = 308/402 (76.62%), Query Frame = 0

Query: 1   MPCHRACLGSR-YHFKFCYTLCTPNCSLLC--------------SPKSLPP---HLPLLP 60
           M C   C+ +   HF+ C +LC+P CS LC               P+  PP    LP LP
Sbjct: 1   MSCQSNCVPTTPSHFQVCTSLCSPKCSGLCFGLPKVPIVVLPRPQPRPPPPPPLPLPQLP 60

Query: 61  SSGNPPRPHPSGK-TLLLLSLISTATFIVAIFMVCLLVKKLKEYRNSRQNG-PPLFDVHR 120
           SS  PP+ H  G+ TLLLLSLI+TAT + AIFM+CLL KK +EYRNSR N  P LFDV  
Sbjct: 61  SSIVPPQTHTPGRSTLLLLSLITTATLVAAIFMLCLLFKKFREYRNSRHNRLPVLFDVQG 120

Query: 121 ENLPDEDEEAPVLDHHVWYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDD 180
           ENLPD DEE PV+DHHVWYINTVGLQQS+IDSITMFKYRKDEKLI+GSDCSICLGEFQDD
Sbjct: 121 ENLPDSDEEEPVIDHHVWYINTVGLQQSVIDSITMFKYRKDEKLIDGSDCSICLGEFQDD 180

Query: 181 ESLRLLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSNSAAPNLVPTEPI-RNQPPAIEV 240
           ESLRLLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLS+S AP+L P EPI RNQP  IE 
Sbjct: 181 ESLRLLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSDSVAPSLAPIEPIRRNQPEPIEN 240

Query: 241 TQMETEAVEEENRGDANSGEGGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIR 300
           TQMETE +EEEN  D NSGE G     NSEI IK LS TLS+L +N   EAVEDEGQ +R
Sbjct: 241 TQMETETIEEENGVDENSGEVGRE---NSEILIKRLSRTLSNLNQNS--EAVEDEGQGLR 300

Query: 301 RSVSMDSVSAMAIYQATAKIQIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNL 360
           RSVSMDSVSAMAIYQ T KIQIPEEG SS+G L+EVK+SVSS+N       +GNQSLYNL
Sbjct: 301 RSVSMDSVSAMAIYQVTTKIQIPEEGCSSNGTLMEVKDSVSSKN-------LGNQSLYNL 360

Query: 361 VKSIPFGRSVQKGGISMKMLSSGRRKIHSARHYRSQSSVLPM 382
           VKSI FG S+QKGGIS+K  SSGRRK+  ARHYRSQSSVLPM
Sbjct: 361 VKSISFGHSMQKGGISIKRFSSGRRKLQWARHYRSQSSVLPM 390

BLAST of CmaCh03G008220 vs. ExPASy TrEMBL
Match: A0A1S3B1E6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103484949 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.3e-136
Identity = 279/402 (69.40%), Postives = 308/402 (76.62%), Query Frame = 0

Query: 1   MPCHRACLGSR-YHFKFCYTLCTPNCSLLC--------------SPKSLPP---HLPLLP 60
           M C   C+ +   HF+ C +LC+P CS LC               P+  PP    LP LP
Sbjct: 1   MSCQSNCVPTTPSHFQVCTSLCSPKCSGLCFGLPKVPIVVLPRPQPRPPPPPPLPLPQLP 60

Query: 61  SSGNPPRPHPSGK-TLLLLSLISTATFIVAIFMVCLLVKKLKEYRNSRQNG-PPLFDVHR 120
           SS  PP+ H  G+ TLLLLSLI+TAT + AIFM+CLL KK +EYRNSR N  P LFDV  
Sbjct: 61  SSIVPPQTHTPGRSTLLLLSLITTATLVAAIFMLCLLFKKFREYRNSRHNRLPVLFDVQG 120

Query: 121 ENLPDEDEEAPVLDHHVWYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDD 180
           ENLPD DEE PV+DHHVWYINTVGLQQS+IDSITMFKYRKDEKLI+GSDCSICLGEFQDD
Sbjct: 121 ENLPDSDEEEPVIDHHVWYINTVGLQQSVIDSITMFKYRKDEKLIDGSDCSICLGEFQDD 180

Query: 181 ESLRLLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSNSAAPNLVPTEPI-RNQPPAIEV 240
           ESLRLLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLS+S AP+L P EPI RNQP  IE 
Sbjct: 181 ESLRLLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSDSVAPSLAPIEPIRRNQPEPIEN 240

Query: 241 TQMETEAVEEENRGDANSGEGGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIR 300
           TQMETE +EEEN  D NSGE G     NSEI IK LS TLS+L +N   EAVEDEGQ +R
Sbjct: 241 TQMETETIEEENGVDENSGEVGRE---NSEILIKRLSRTLSNLNQNS--EAVEDEGQGLR 300

Query: 301 RSVSMDSVSAMAIYQATAKIQIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNL 360
           RSVSMDSVSAMAIYQ T KIQIPEEG SS+G L+EVK+SVSS+N       +GNQSLYNL
Sbjct: 301 RSVSMDSVSAMAIYQVTTKIQIPEEGCSSNGTLMEVKDSVSSKN-------LGNQSLYNL 360

Query: 361 VKSIPFGRSVQKGGISMKMLSSGRRKIHSARHYRSQSSVLPM 382
           VKSI FG S+QKGGIS+K  SSGRRK+  ARHYRSQSSVLPM
Sbjct: 361 VKSISFGHSMQKGGISIKRFSSGRRKLQWARHYRSQSSVLPM 390

BLAST of CmaCh03G008220 vs. NCBI nr
Match: XP_022978507.1 (RING-H2 finger protein ATL54-like [Cucurbita maxima])

HSP 1 Score: 756.9 bits (1953), Expect = 8.4e-215
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60
           MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL
Sbjct: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60

Query: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI 120
           SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI
Sbjct: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI 120

Query: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180
           NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT
Sbjct: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180

Query: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG 240
           WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG
Sbjct: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG 240

Query: 241 GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300
           GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ
Sbjct: 241 GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300

Query: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360
           IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS
Sbjct: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360

Query: 361 SGRRKIHSARHYRSQSSVLPM 382
           SGRRKIHSARHYRSQSSVLPM
Sbjct: 361 SGRRKIHSARHYRSQSSVLPM 381

BLAST of CmaCh03G008220 vs. NCBI nr
Match: XP_022949771.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata])

HSP 1 Score: 728.8 bits (1880), Expect = 2.4e-206
Identity = 366/381 (96.06%), Postives = 371/381 (97.38%), Query Frame = 0

Query: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60
           MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPK  PPHLPLLPSSGNPPRPHPSGKTLLLL
Sbjct: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKPPPPHLPLLPSSGNPPRPHPSGKTLLLL 60

Query: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDEDEEAPVLDHHVWYI 120
           SLISTAT +VAIFM+CLLVKKLKEYRNSRQNGPPLFDVHRENLPD+DEE PVLDHHVWYI
Sbjct: 61  SLISTATLVVAIFMLCLLVKKLKEYRNSRQNGPPLFDVHRENLPDDDEEGPVLDHHVWYI 120

Query: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180
           NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT
Sbjct: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180

Query: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEG 240
           WLRSHKNCPLCRAPVLSNSAAPNLVP EPI NQPPAIE TQMETEAVEEENRG+ NSGEG
Sbjct: 181 WLRSHKNCPLCRAPVLSNSAAPNLVPIEPIGNQPPAIEDTQMETEAVEEENRGNENSGEG 240

Query: 241 GSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300
           GSNSGGNSEIPIKGLS TLSDLTENQ+REAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ
Sbjct: 241 GSNSGGNSEIPIKGLSRTLSDLTENQVREAVEDEGQAIRRSVSMDSVSAMAIYQATAKIQ 300

Query: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360
           IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS
Sbjct: 301 IPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKMLS 360

Query: 361 SGRRKIHSARHYRSQSSVLPM 382
           SGRRKIH ARHYRSQSSVLPM
Sbjct: 361 SGRRKIHWARHYRSQSSVLPM 381

BLAST of CmaCh03G008220 vs. NCBI nr
Match: XP_023544216.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.2 bits (1868), Expect = 6.0e-205
Identity = 367/384 (95.57%), Postives = 371/384 (96.61%), Query Frame = 0

Query: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60
           MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPK  PPHLPLLPSSGNPPRPHPSGKTLLLL
Sbjct: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKPPPPHLPLLPSSGNPPRPHPSGKTLLLL 60

Query: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDED---EEAPVLDHHV 120
           SLISTAT +VAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPD+D   EE PVLDHHV
Sbjct: 61  SLISTATLVVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDDDDDEEEGPVLDHHV 120

Query: 121 WYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPC 180
           WYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPC
Sbjct: 121 WYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPC 180

Query: 181 IDTWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANS 240
           IDTWLRSHKNCPLCRAPVLSNSAAPNLVP EPIRNQPPAIE TQMETEAVEEENRG+ NS
Sbjct: 181 IDTWLRSHKNCPLCRAPVLSNSAAPNLVPIEPIRNQPPAIEDTQMETEAVEEENRGNENS 240

Query: 241 GEGGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATA 300
           GEGGSNSGGNSEIPIKGLS TLSDLTENQIREAV+DEGQAIRRSVSMDSVSAMAIYQATA
Sbjct: 241 GEGGSNSGGNSEIPIKGLSRTLSDLTENQIREAVQDEGQAIRRSVSMDSVSAMAIYQATA 300

Query: 301 KIQIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMK 360
           KIQIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMK
Sbjct: 301 KIQIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMK 360

Query: 361 MLSSGRRKIHSARHYRSQSSVLPM 382
           MLSS RRKIH ARHYRSQSSVLPM
Sbjct: 361 MLSSSRRKIHWARHYRSQSSVLPM 384

BLAST of CmaCh03G008220 vs. NCBI nr
Match: KAG6604048.1 (RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 721.5 bits (1861), Expect = 3.9e-204
Identity = 365/382 (95.55%), Postives = 369/382 (96.60%), Query Frame = 0

Query: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60
           MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPK  PPHLPLLPSSGNPPRPHPSGKTLLLL
Sbjct: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKPPPPHLPLLPSSGNPPRPHPSGKTLLLL 60

Query: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDED-EEAPVLDHHVWY 120
           SLISTAT +VAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPD+D EE PVLDHHVWY
Sbjct: 61  SLISTATLVVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDDDEEEGPVLDHHVWY 120

Query: 121 INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID 180
           INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID
Sbjct: 121 INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID 180

Query: 181 TWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGE 240
           TWLRSHKNCPLCRAPVLSNS APNLVP EPIRNQPPAIE TQMETEAVEEENRG+ NSGE
Sbjct: 181 TWLRSHKNCPLCRAPVLSNSTAPNLVPIEPIRNQPPAIEDTQMETEAVEEENRGNENSGE 240

Query: 241 GGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKI 300
           GGSN GGNSEIPIKGLS TLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKI
Sbjct: 241 GGSNIGGNSEIPIKGLSRTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKI 300

Query: 301 QIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKML 360
           QIPEEGSSSSGALVEV NSVSSRN+PFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKML
Sbjct: 301 QIPEEGSSSSGALVEVNNSVSSRNLPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKML 360

Query: 361 SSGRRKIHSARHYRSQSSVLPM 382
           SSGRRKIH ARHYRSQSSVLPM
Sbjct: 361 SSGRRKIHWARHYRSQSSVLPM 382

BLAST of CmaCh03G008220 vs. NCBI nr
Match: KAG7034214.1 (RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 719.9 bits (1857), Expect = 1.1e-203
Identity = 364/382 (95.29%), Postives = 369/382 (96.60%), Query Frame = 0

Query: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLL 60
           MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPK  PPHLPLLPSSGNPPRPHPSGKTLLLL
Sbjct: 1   MPCHRACLGSRYHFKFCYTLCTPNCSLLCSPKPPPPHLPLLPSSGNPPRPHPSGKTLLLL 60

Query: 61  SLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDED-EEAPVLDHHVWY 120
           SLISTAT +VAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPD+D EE PVLDHHVWY
Sbjct: 61  SLISTATLVVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPDDDEEEGPVLDHHVWY 120

Query: 121 INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID 180
           INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID
Sbjct: 121 INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID 180

Query: 181 TWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGE 240
           TWLRSHKNCPLCRAPVLSNS APNLVP EPIRNQPPAIE TQMETEAVEEENRG+ NSGE
Sbjct: 181 TWLRSHKNCPLCRAPVLSNSTAPNLVPIEPIRNQPPAIEDTQMETEAVEEENRGNENSGE 240

Query: 241 GGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKI 300
           GGSN GGNSEIPIKGLS TLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKI
Sbjct: 241 GGSNIGGNSEIPIKGLSRTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATAKI 300

Query: 301 QIPEEGSSSSGALVEVKNSVSSRNVPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKML 360
           QIPEEGSSSSGALVEV NSVSSRN+PFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKML
Sbjct: 301 QIPEEGSSSSGALVEVNNSVSSRNLPFPKRGVGNQSLYNLVKSIPFGRSVQKGGISMKML 360

Query: 361 SSGRRKIHSARHYRSQSSVLPM 382
           SSGRRKIH ARHYRSQSSVLP+
Sbjct: 361 SSGRRKIHWARHYRSQSSVLPI 382

BLAST of CmaCh03G008220 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 150.6 bits (379), Expect = 2.6e-36
Identity = 78/159 (49.06%), Postives = 104/159 (65.41%), Query Frame = 0

Query: 47  PPRPHPSGKTLLL-LSLISTATFIVAIFMVCLLVKKLKEYRNSRQNGPPLFDVHRENLPD 106
           PP P P   +LLL  S+    + I+ +F+V  L   + + R          D +++   D
Sbjct: 30  PPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQEDED 89

Query: 107 EDEEAPVLD-HHVWYINTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLR 166
           E EE    D HHVW I TVGL +S I+SIT+  ++K E +I+G++CS+CL EF++DESLR
Sbjct: 90  ETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCLNEFEEDESLR 149

Query: 167 LLPKCSHAFHVPCIDTWLRSHKNCPLCRAPVLSNSAAPN 204
           LLPKCSHAFH+ CIDTWL SHKNCPLCRAPVL  +  P+
Sbjct: 150 LLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPH 188

BLAST of CmaCh03G008220 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.8e-34
Identity = 101/324 (31.17%), Postives = 156/324 (48.15%), Query Frame = 0

Query: 3   CHRACLGSRYHFKFCYTLCTPNCSLLCSPKSLPPHLPLLPSSGNPPRPHPSGKTLLLLSL 62
           C   C+       +C + C   C  +C          ++ S  +PP        L  + +
Sbjct: 75  CKNNCIQELKTLGYCSSYCLDECPSICE--------IVVISGLSPPAEIHHDDNLKRILI 134

Query: 63  ISTATFIVAIFMVCLLVKKLKEYRNSRQN--GPPLFDVHRENLPDEDEEAPVL-DHHVWY 122
           IS ++ I  +F++ LLV   K Y   R++      + +      D D  APV+ DH +W+
Sbjct: 135 ISASSIITTLFLLTLLVLCFKFYYRRRRSITTSRRWSMEEARNWDFDGPAPVIVDHPIWH 194

Query: 123 INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID 182
           I T+GL  ++I SI + +Y K + ++EG+DCS+CL EF+++E+LRLLPKC HAFH+ CID
Sbjct: 195 IRTIGLNPTVISSIKVCQYSKKDGVVEGTDCSVCLSEFEEEETLRLLPKCKHAFHLYCID 254

Query: 183 TWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGE 242
           TWLRSH NCPLCRAP++  +          I +    +E    E   +  E  GD    E
Sbjct: 255 TWLRSHTNCPLCRAPIVEANTM--------IDDHSEGLE----EISVMIPEENGDDTDEE 314

Query: 243 GGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDSVSAMAIYQATA-- 302
                 G                  N  R+  E + Q +RRSVS+DS+S + + +     
Sbjct: 315 IEEERDG---------------FVSNISRDHGESQQQRVRRSVSLDSLSGLRVSEVVVGR 363

Query: 303 -KIQIPEEGSSSSGALVEVKNSVS 321
            K ++    +  SG    +K S+S
Sbjct: 375 EKEKLKRGNNIGSGRSSLLKRSIS 363

BLAST of CmaCh03G008220 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 139.8 bits (351), Expect = 4.5e-33
Identity = 99/261 (37.93%), Postives = 138/261 (52.87%), Query Frame = 0

Query: 20  LCTPNCSLLCSPK----SLPPHLPLLPSSGNP-PRPHPSGKTLLL--------LSLISTA 79
           +C P C   C P+    ++ P LP   SS  P P P PS   + L        L  IS  
Sbjct: 27  VCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISII 86

Query: 80  TFIVAIFMVCL----LVKKLKEYRNSRQNGPPLFDVHREN---LPDEDEEAPVLDHHVWY 139
           T   A+  + L    LV K      +R N       + +N   + +E ++   +DH +W 
Sbjct: 87  TITGAVLAILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQVDHPIWL 146

Query: 140 INTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCID 199
           I T GLQQSII+SIT+  Y++ + LIE +DC +CL EF++DESLRLLPKC+HAFH+ CID
Sbjct: 147 IRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFHISCID 206

Query: 200 TWLRSHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVT------QMETEAVEEENRG 254
           TWL SH NCPLCRA +   S       T P  + P  +EVT       +E + V+EE+ G
Sbjct: 207 TWLSSHTNCPLCRAGIAMISV------TTPRYSGP--VEVTPGGSGSHLENDGVDEEDHG 266

BLAST of CmaCh03G008220 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 2.0e-28
Identity = 67/163 (41.10%), Postives = 94/163 (57.67%), Query Frame = 0

Query: 124 GLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDTWLR 183
           GL +S+I SIT++KYRK +  +E SDCS+CL EFQ++ESLRLLPKC+HAFHVPCIDTWL+
Sbjct: 133 GLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLK 192

Query: 184 SHKNCPLCRAPVLSNSAAPNLVPTEPIRNQPPAIEVTQMETEAVEEENRGDANSGEGGSN 243
           SH NCPLCRA ++++S                A+E+  +  + +  EN          S 
Sbjct: 193 SHSNCPLCRAFIVTSS----------------AVEIVDLTNQQIVTEN---------NSI 252

Query: 244 SGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMDS 287
           S G+  + +  L     DL  ++ R    +EG   +     DS
Sbjct: 253 STGDDSVVVVNL-----DLENSRSRNETVNEGSTPKPPEMQDS 265

BLAST of CmaCh03G008220 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.7e-27
Identity = 79/202 (39.11%), Postives = 114/202 (56.44%), Query Frame = 0

Query: 121 NTVGLQQSIIDSITMFKYRKDEKLIEGSDCSICLGEFQDDESLRLLPKCSHAFHVPCIDT 180
           N  GL +S+I SIT++KY+  +  ++GSDCS+CL EF+++ESLRLLPKC+HAFH+PCIDT
Sbjct: 113 NGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDT 172

Query: 181 WLRSHKNCPLCRA---PVLSNSAAPNLVPTEPIRNQPPAIEVTQMETE-----AVEEENR 240
           WL+SH NCPLCRA    V + +A+     +  + NQ  +   T   +E     A  E   
Sbjct: 173 WLKSHSNCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQT 232

Query: 241 GDANS-------GEGGSNSGGNSEIPIKGLSLTLSDLTENQIREAVEDEGQAIRRSVSMD 300
           GD +S         G  NS   SE+ +             + RE  +++   IRRSVS++
Sbjct: 233 GDFDSVVVIEDLEIGSRNSDARSELQL-----------PEERRETKDEDSLPIRRSVSLN 292

Query: 301 SVSAMAIYQATAKIQIPEEGSS 308
           S   ++I     +I+  EEG S
Sbjct: 293 SGVVVSIADVLREIE-DEEGES 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LX933.6e-3549.06E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ... [more]
Q8LFY86.3e-3237.93RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Q9SRQ82.8e-2741.10RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q9LF642.3e-2639.11RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q7XLY86.4e-2457.32E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
Match NameE-valueIdentityDescription
A0A6J1IL984.0e-215100.00RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478464 P... [more]
A0A6J1GCY11.2e-20696.06RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453059... [more]
A0A0A0KK071.9e-15174.31RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G499220 PE... [more]
A0A5A7T0B51.3e-13669.40RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B1E61.3e-13669.40RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103484949 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_022978507.18.4e-215100.00RING-H2 finger protein ATL54-like [Cucurbita maxima][more]
XP_022949771.12.4e-20696.06E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata][more]
XP_023544216.16.0e-20595.57E3 ubiquitin-protein ligase RING1-like [Cucurbita pepo subsp. pepo][more]
KAG6604048.13.9e-20495.55RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7034214.11.1e-20395.29RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
AT5G10380.12.6e-3649.06RING/U-box superfamily protein [more]
AT4G33565.11.8e-3431.17RING/U-box superfamily protein [more]
AT1G72220.14.5e-3337.93RING/U-box superfamily protein [more]
AT3G03550.12.0e-2841.10RING/U-box superfamily protein [more]
AT5G17600.11.7e-2739.11RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM01197FANCL_C_2coord: 146..199
e-value: 0.0092
score: -1.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 223..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 236..250
NoneNo IPR availablePANTHERPTHR45676:SF126RING-H2 FINGER PROTEIN ATL54coord: 15..368
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 149..192
e-value: 3.09871E-17
score: 72.7197
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 138..198
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 150..191
e-value: 1.5E-7
score: 41.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 149..192
e-value: 4.9E-14
score: 52.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 150..192
score: 12.754832
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 125..200
e-value: 4.2E-23
score: 82.3
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 15..368

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G008220.1CmaCh03G008220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane