CmaCh03G008180 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G008180 vs. ExPASy Swiss-Prot
Match: Q1HFZ0 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Mus musculus OX=10090 GN=Nsun2 PE=1 SV=2)

HSP 1 Score: 417.9 bits (1073), Expect = 2.9e-115
Identity = 271/778 (34.83%), Postives = 405/778 (52.06%), Query Frame = 0

Query: 106 QSHAFNEYYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQ 165
           ++  F  YY+E  IV   EWD F+E LR+ LPA  RI        EI   L+N + K L+
Sbjct: 44  ENKLFEHYYQELKIVPEGEWDQFMESLREPLPATLRITGYKSHAKEILHCLKNKYFKELE 103

Query: 166 SEVTDGDEAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEA 225
               DG +    +PLSWYP+ LAWH+N SR  LRK+  L +FH+FL  E E GNI+RQEA
Sbjct: 104 DLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPLLAKFHQFLVSETESGNISRQEA 163

Query: 226 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCN 285
           VSM+PPL L+V P+H +LDMCAAPGSKT QL+E++H +      P G V+AND D +RC 
Sbjct: 164 VSMIPPLLLNVEPHHKILDMCAAPGSKTTQLIEMLH-ADMSVPFPEGFVIANDVDNKRCY 223

Query: 286 LLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVP 345
           LL+HQ KR+ +  ++V NH+A   P                +++     L +DR+LCDVP
Sbjct: 224 LLVHQAKRLSSPCIMVVNHDASSIPRLTV------------DVDGRKEILFYDRILCDVP 283

Query: 346 CSGDGTLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEA 405
           CSGDGT+RK  D+W++W +     LHGLQ++I  RGA  L  GGRMVYSTCS+NPVE+EA
Sbjct: 284 CSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPVEDEA 343

Query: 406 VVAELLRKSEGSLEVVDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIP 465
           V+A LL KSEG+LE+ DVS ELP L   PG+ +WKV  R G W A + +V   R +   P
Sbjct: 344 VIAALLEKSEGALELADVSAELPGLKWMPGVSQWKVMTRDGQWFADWHEVPQGRHTQIRP 403

Query: 466 SMFPFGRGRKNQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMR 525
           +MFP                                  PT     + +++    +ERC+R
Sbjct: 404 TMFP----------------------------------PT-----DLEKLQAMHLERCLR 463

Query: 526 IVPHDQNSGAFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSAS 585
           I+PH QN+G FF+AVL K AP+P    +P     K  N  +  + P+      +  V+A+
Sbjct: 464 ILPHHQNTGGFFVAVLVKKAPMPWNKRQP-----KVQNKSAEAREPR-----VSSHVAAT 523

Query: 586 VGMDESPSEVGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLE 645
            G     SE+                                             E+ + 
Sbjct: 524 EGNPSDQSEL---------------------------------------------ESQMI 583

Query: 646 VNLIDNEVNVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLF- 705
               D+E   +N E T       ES +  DG   P  S   +K+++ G  +  DP V   
Sbjct: 584 TGAGDSE-TAHNTENT-------ESNEKKDGVCGPPPS---KKMKLFGFKE--DPFVFIP 643

Query: 706 KDETVIDSIKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLK 765
           +D+ +   I+ FY ++ +FP   +L+TR T+    +++Y VSK +++VL  N    +++K
Sbjct: 644 EDDPLFPPIEKFYALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMK 689

Query: 766 ITSIGLKMFERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIK 825
           + + G+K++ R +S  G    C+FR+  EG+  + P+I  +I+T+S  D K L+  ++  
Sbjct: 704 VINTGIKVWCRNNS--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQENPF 689

Query: 826 YADFADAAFGEKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 882
           +   +  A+ +    L  G  V++     + + D +   +  I +  W+G++S+   V
Sbjct: 764 FRKLSSEAYSQ-VKDLAKG-SVVLKYEPDSANPDTL---QCPIVLCGWRGKASIRTFV 689

BLAST of CmaCh03G008180 vs. ExPASy Swiss-Prot
Match: Q5ZLV4 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Gallus gallus OX=9031 GN=NSUN2 PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.4e-114
Identity = 273/822 (33.21%), Postives = 409/822 (49.76%), Query Frame = 0

Query: 61  GGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYYKEQGIV 120
           G RAR +R   +  +    +          D + G +  +     ++  F  YY+EQ IV
Sbjct: 2   GRRARDRRRQLQPQQR---RERSGGGGGGGDDQAGWAGGYPEIVKENELFERYYREQRIV 61

Query: 121 TAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEAGAIRPL 180
              EWD F+  LR+ LPA  RI        EI   L+  + + LQ    DG +    + L
Sbjct: 62  PDGEWDAFMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQKVEMPQAL 121

Query: 181 SWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNH 240
           SWYP+ LAWH+N SR  LRK+  LERFH+FL  E E GNI+RQEAVSM+PPL L+V+P+H
Sbjct: 122 SWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNVNPDH 181

Query: 241 YVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRMCTSNLI 300
            +LDMCAAPGSKT QL+E++H        P G V+AND D +RC LL+HQ KR+ +  ++
Sbjct: 182 KILDMCAAPGSKTAQLIEMLHADMNV-PFPKGFVIANDVDNKRCYLLVHQAKRLNSPCIM 241

Query: 301 VTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRKAPDIWR 360
           V NH+A   P  +             +++     L +DR+LCDVPCSGDGT+RK  D+W+
Sbjct: 242 VVNHDASSIPNLQV------------DVDGRKETLFYDRILCDVPCSGDGTMRKNIDVWK 301

Query: 361 RWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSEGSLEV 420
           +W +     LHGLQ++I  RG   L  GGRMVYSTCS+NP+ENEAV+A LL KS+G+LE+
Sbjct: 302 KWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQGALEL 361

Query: 421 VDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPFGRGRKNQADK 480
            DVS+ELP L R PG+ KWKV  + G W   +K V  +RQ+   P+MF            
Sbjct: 362 ADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMF------------ 421

Query: 481 SHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGAFFIAV 540
                                      P+ E +++    +ERC+RI+PH QN+G FF+AV
Sbjct: 422 ---------------------------PIKEEEKLKAMNLERCIRILPHHQNTGGFFVAV 481

Query: 541 LRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEVGIDSN 600
           L K +P+P    +P  H           KLP               G  E   +  + + 
Sbjct: 482 LIKKSPMPWNKRQPKVHQ----------KLP---------------GKTE---DTEVTAT 541

Query: 601 NQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNVNNLEI 660
           N GD                          + D  +K     D E   +    N      
Sbjct: 542 NAGD-------------------------GSEDATEKPTLAEDEEPKKVQELQN------ 601

Query: 661 TAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKTFYGIN 720
                   +++Q   G   P  S   +K+++ G +K    V L +D+ +   I+ FY ++
Sbjct: 602 -------SDTEQSKKGVCGPPPS---KKMKLFG-FKEDPFVFLPEDDPLFLPIQKFYALD 661

Query: 721 EAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFERQSSRE 780
            +FP   +L+TR T     +++Y VSK +++VL  N    +++K+ + G+K++ R S  +
Sbjct: 662 PSFPKM-NLLTR-TQEGKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWSRNS--D 686

Query: 781 GSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGEKASSL 840
           G    C+FR+  EG+  + P+I  +I+ +   D K L+  ++   + F+      K   +
Sbjct: 722 GEQFGCAFRLAQEGIYTLYPFIHARIVNVCIEDVKILLTQENPFLSKFSSET-QRKVKDM 686

Query: 841 MLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 882
            +G  V+          D+    +  I +  W+G++SL   V
Sbjct: 782 AMGSIVLKYEPDPEKPDDL----QCPIVLCGWQGKTSLRAFV 686

BLAST of CmaCh03G008180 vs. ExPASy Swiss-Prot
Match: Q08J23 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Homo sapiens OX=9606 GN=NSUN2 PE=1 SV=2)

HSP 1 Score: 410.2 bits (1053), Expect = 5.9e-113
Identity = 274/832 (32.93%), Postives = 411/832 (49.40%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQ---SQSHAFN 114
           MG + RG R + Q+           +    +  +    K G +   G +     ++  F 
Sbjct: 1   MGRRSRGRRLQQQQ-----------RPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFE 60

Query: 115 EYYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDG 174
            YY+E  IV   EW  F++ LR+ LPA  RI        EI   L+N + K L+    DG
Sbjct: 61  HYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDG 120

Query: 175 DEAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPP 234
            +    +PLSWYP+ LAWH+N SR  LRK+  LE+FH+FL  E E GNI+RQEAVSM+PP
Sbjct: 121 QKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPP 180

Query: 235 LFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQT 294
           L L+V P+H +LDMCAAPGSKT QL+E++H        P G V+AND D +RC LL+HQ 
Sbjct: 181 LLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQA 240

Query: 295 KRMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGT 354
           KR+ +  ++V NH+A   P  +             +++     L +DR+LCDVPCSGDGT
Sbjct: 241 KRLSSPCIMVVNHDASSIPRLQI------------DVDGRKEILFYDRILCDVPCSGDGT 300

Query: 355 LRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELL 414
           +RK  D+W++W +     LHGLQ++I  RGA  L  GGRMVYSTCS+NP+E+EAV+A LL
Sbjct: 301 MRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLL 360

Query: 415 RKSEGSLEVVDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPFG 474
            KSEG+LE+ DVSNELP L   PG+ +WKV  + G W   +  V   R +   P+MFP  
Sbjct: 361 EKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPK 420

Query: 475 RGRKNQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQ 534
              K QA                                         +ERC+RI+PH Q
Sbjct: 421 DPEKLQA---------------------------------------MHLERCLRILPHHQ 480

Query: 535 NSGAFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDES 594
           N+G FF+AVL K + +P    +P          +S    P ++T G   D S      ES
Sbjct: 481 NTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKL----ES 540

Query: 595 PSEVGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDN 654
           PS  G                                                       
Sbjct: 541 PSFTG------------------------------------------------------- 600

Query: 655 EVNVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLF-KDETVI 714
                + EI    ++LE +    DG   P  S   +K+++ G  +  DP V   +D+ + 
Sbjct: 601 ---TGDTEIAHATEDLENNGSKKDGVCGPPPS---KKMKLFGFKE--DPFVFIPEDDPLF 660

Query: 715 DSIKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGL 774
             I+ FY ++ +FP   +L+TR T+    +++Y VSK +++VL  N    +++K+ + G+
Sbjct: 661 PPIEKFYALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGI 690

Query: 775 KMFERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFAD 834
           K++ R +S  G    C+FR+  EG+  + P+I  +I+T+S  D K L+  ++  +   + 
Sbjct: 721 KVWCRNNS--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSS 690

Query: 835 AAFGEKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 882
             + + A  L  G  +++     + + D +   +  I +  W+G++S+   V
Sbjct: 781 ETYSQ-AKDLAKG-SIVLKYEPDSANPDAL---QCPIVLCGWRGKASIRTFV 690

BLAST of CmaCh03G008180 vs. ExPASy Swiss-Prot
Match: Q28E61 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus tropicalis OX=8364 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.1e-109
Identity = 274/830 (33.01%), Postives = 410/830 (49.40%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MG + R  R R QR+  + S     +R K    +A +        +     ++  F  YY
Sbjct: 1   MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAAWEC------GYPEIIKENKLFEHYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           +E  IV   EWD F+  LR+ LPA  RI        EI   L+  + K LQ    DG + 
Sbjct: 61  QELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVDGQKI 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
            A +PLSWYP+ LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+PP+ L
Sbjct: 121 EAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
            V P+H +LDMCAAPGSKT Q++E++H        P G V+AND D +RC LL+HQ KR+
Sbjct: 181 KVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQAKRL 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
            +  ++V NH+A   P                E N     L +DR+LCDVPCSGDGTLRK
Sbjct: 241 NSPCIMVVNHDASSIPRLLV------------ENNGSREVLYYDRILCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
             D+W++W +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+A LL KS
Sbjct: 301 NIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPFGRGR 474
           EGSLE+ DV++E+P L   PG+ +WKV  + G W   ++ +   R +   P+MFP     
Sbjct: 361 EGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFP----- 420

Query: 475 KNQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSG 534
                     +L+  +LN                             RCMRI+PH QN+G
Sbjct: 421 -----PKDEEKLKSMNLN-----------------------------RCMRILPHHQNTG 480

Query: 535 AFFIAVLRKVAPLPAATEKPSYHNLKP--ANDKSRCKLPQNITNGANVDVSASVGMDESP 594
            FF+AVL K AP+P    +P      P  A D S    P+ +          S GM + P
Sbjct: 481 GFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELV----KAVTENSAGMADEP 540

Query: 595 SEVGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNE 654
           +   +D+ N       + KP                                        
Sbjct: 541 A---VDTEN------GETKPC--------------------------------------- 600

Query: 655 VNVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDS 714
                        N  +S + D     P      +K+++ G +K    V + +D+ + D 
Sbjct: 601 ------------TNQSDSSKTDIVCCPPP----SKKMKLFG-FKEDPFVFVSEDDPIFDP 660

Query: 715 IKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKM 774
           I+TFY ++ +FP   +L+TR T     +++Y VSK +++VL  N    +++K+ + G+K+
Sbjct: 661 IQTFYALDPSFPK-KNLLTR-TQEGKKRQLYMVSKELRNVLLHN---SEKMKVINTGIKV 691

Query: 775 FERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAA 834
             R  + +G    C++R+  EG+  + P+I  +I+T+S  D K L+  ++   + F+   
Sbjct: 721 LCR--NNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKFSKET 691

Query: 835 FGEKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 882
             ++A++  +G  V+           +    +  I +  W+G++S+   V
Sbjct: 781 -QKQANNFDMGSIVLKYEPDPQEPETL----QCPIVLCGWRGKTSIRSFV 691

BLAST of CmaCh03G008180 vs. ExPASy Swiss-Prot
Match: Q4V7N2 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus laevis OX=8355 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.6e-108
Identity = 265/816 (32.48%), Postives = 403/816 (49.39%), Query Frame = 0

Query: 72  RESRENVWKRSKPDSNSANDTKDGN-----SNHWGPFQSQSHAFNEYYKEQGIVTAEEWD 131
           R++R N  +R++  S +  + +           +     ++  F  YY+E  IV   EWD
Sbjct: 3   RKNRRNRQRRTEQRSPAEEERRKAREQAAWEGGYPEIIKENKLFEHYYQELKIVPDGEWD 62

Query: 132 TFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEAGAIRPLSWYPDN 191
            F++ LR+ LPA  RI        EI   L+  + K L     DG +  A +PLSWYP+ 
Sbjct: 63  KFMDALREPLPATIRITGYKSHAKEILHCLKEKYFKELPDIEVDGQKIEAPQPLSWYPEE 122

Query: 192 LAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNHYVLDMC 251
           LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+PP+ L+V P+H +LDMC
Sbjct: 123 LAWHTNLSRKIIRKSPELEKFHQFLVSETESGNISRQEAVSMIPPVLLNVQPHHKILDMC 182

Query: 252 AAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRMCTSNLIVTNHEA 311
           AAPGSKT Q++E++H        P G V+AND D +RC LL+HQ KR+ +  ++V NH+A
Sbjct: 183 AAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDA 242

Query: 312 QHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRKAPDIWRRWNSGV 371
              P                E N     L +DR+LCDVPCSGDGT+RK  D+W++W +  
Sbjct: 243 SSIPRLLI------------ENNGSREVLYYDRILCDVPCSGDGTMRKNIDVWKKWTTLN 302

Query: 372 GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSEGSLEVVDVSNE 431
              LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+  LL KSEGSLE+ DV++E
Sbjct: 303 SLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIVSLLDKSEGSLELADVASE 362

Query: 432 LPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPFGRGRKNQADKSHNSEL 491
           LP L   PG+ +W+V  + G W   ++ V   R +   P+MFP               +L
Sbjct: 363 LPGLKWMPGITQWRVMTKEGQWFEKWEDVPTSRHTQIRPTMFPL----------KDEEKL 422

Query: 492 RGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGAFFIAVLRKVAP 551
           +  +LN                             RCMRI+PH QN+G FF+AVL K AP
Sbjct: 423 KSMNLN-----------------------------RCMRILPHHQNTGGFFVAVLIKKAP 482

Query: 552 LPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEVGIDSNNQGDFN 611
           +P    +P      P +       P+ +   A  D+SA         E  +D+ N     
Sbjct: 483 MPWNKRQPKLQRRPPVSVCDASVAPEIVK--AVADISAIA------DEPAVDAEN----- 542

Query: 612 SADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNVNNLEITAIEDN 671
             + KP                                                     N
Sbjct: 543 -GETKPC---------------------------------------------------TN 602

Query: 672 LEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKTFYGINEAFPLF 731
              S + D     P      +K+++ G +K    V L +D+ + + I+ FY ++ +FP  
Sbjct: 603 QSGSSKTDSVCCPPP----SKKMKLFG-FKEDPFVFLSEDDPIFEPIQKFYALDPSFPK- 662

Query: 732 GHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFERQSSREGSSAPC 791
            +L+TR T     +++Y VSK +++VL  N    +++K+ + G+K+  R  + +G    C
Sbjct: 663 KNLLTR-TQEGKKRQLYMVSKELRNVLLHN---SEKMKVINTGIKVLCR--NNDGEQYGC 684

Query: 792 SFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGEKASSLMLGCCV 851
           ++R+  EG+  + P+I  +ILT+S  D K L+  ++   + F+     ++A++L +G  V
Sbjct: 723 AYRLAQEGIYSLYPFINARILTVSVEDIKVLLTQENPFLSKFSKET-QKQANNLDMGSIV 684

Query: 852 IVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 882
           +           +    +  I +  W+G++S+   V
Sbjct: 783 LKYEPDPQQPETL----QCPIVLCGWRGKTSIRSFV 684

BLAST of CmaCh03G008180 vs. ExPASy TrEMBL
Match: A0A6J1IQ20 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478413 PE=3 SV=1)

HSP 1 Score: 1703.7 bits (4411), Expect = 0.0e+00
Identity = 847/847 (100.00%), Postives = 847/847 (100.00%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 847

BLAST of CmaCh03G008180 vs. ExPASy TrEMBL
Match: A0A6J1IL24 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478413 PE=3 SV=1)

HSP 1 Score: 1674.8 bits (4336), Expect = 0.0e+00
Identity = 837/847 (98.82%), Postives = 837/847 (98.82%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 837

BLAST of CmaCh03G008180 vs. ExPASy TrEMBL
Match: A0A6J1GFP9 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1670.2 bits (4324), Expect = 0.0e+00
Identity = 829/847 (97.87%), Postives = 836/847 (98.70%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTN FNASAAGSELN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHL+GSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCKLPQ ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           G+DSNNQGDFNSADNKPACEFPVQDIEG EGLDSNAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLF+DETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNF VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGS APCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KAS+LMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 847

BLAST of CmaCh03G008180 vs. ExPASy TrEMBL
Match: A0A6J1GFM2 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1641.3 bits (4249), Expect = 0.0e+00
Identity = 819/847 (96.69%), Postives = 826/847 (97.52%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTN FNASAAGSELN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHL+GSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCKLPQ ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           G+DSNNQGDFNSADNKPACEFPVQDIEG EGLDSNAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLF+DETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFRDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNF VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGS APCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KAS+LMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 837

BLAST of CmaCh03G008180 vs. ExPASy TrEMBL
Match: A0A6J1HGN2 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463320 PE=3 SV=1)

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 720/848 (84.91%), Postives = 767/848 (90.45%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQR HFRESRENVWKR KPD NSAN  KDGN NHW PF+SQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTAEEWDTFIE LRK LPAAFRINSSGQFCGEIRAQLQNDF KSLQ+EVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
            AIRPL WYPDNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CT+NLIVTNHEAQHFPGCRA+TNFF+AS AG+EL   +TQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
            GSLE+VDVSNELPQLVRRPGLKKWKV D+GVW ASY+QV++  QSVAIP MFP GRGRK
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 534
           +    +HNSELRGNH +G  GSSVDVSEPTMDPVH FD EVCEFPIERCMRIVPHDQN+G
Sbjct: 421 SD---THNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480

Query: 535 AFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSE 594
           AFF+AVLRKVAPLPAATEK S  N K AND+S  KL Q IT+  +VD++A+ GM ES SE
Sbjct: 481 AFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSE 540

Query: 595 VGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVN 654
           V ID NNQ D NSA+N P C+ PVQD +  +GLDS   DG+DKQFSEAD+EV  ++NE  
Sbjct: 541 VEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENETT 600

Query: 655 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIK 714
            N LEITAI++N EESKQP DGEMDPKK   KRKLQIQGKW+GVDPVVLFKDETVID IK
Sbjct: 601 ENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIK 660

Query: 715 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFE 774
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+S SVKDVLELNFS GQQLKITSIGLKMFE
Sbjct: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFE 720

Query: 775 RQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 834
           RQSSREGSSAPC+FRI+SEGLPV+LPYITKQILTMSPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFG 780

Query: 835 EKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 894
           EKAS+LMLGCCVIVLGT GNTSSDVIKVDESTIAIGCWKGR+SLSVMVTA DCQELL RL
Sbjct: 781 EKASNLMLGCCVIVLGT-GNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRL 838

Query: 895 SNRLGNDV 902
           SNRLGND+
Sbjct: 841 SNRLGNDL 838

BLAST of CmaCh03G008180 vs. NCBI nr
Match: XP_022978420.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1703.7 bits (4411), Expect = 0.0e+00
Identity = 847/847 (100.00%), Postives = 847/847 (100.00%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 847

BLAST of CmaCh03G008180 vs. NCBI nr
Match: XP_022978422.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1674.8 bits (4336), Expect = 0.0e+00
Identity = 837/847 (98.82%), Postives = 837/847 (98.82%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 837

BLAST of CmaCh03G008180 vs. NCBI nr
Match: KAG6604044.1 (RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034210.1 tRNA (cytosine(34)-C(5))-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1672.5 bits (4330), Expect = 0.0e+00
Identity = 831/847 (98.11%), Postives = 838/847 (98.94%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVH NHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHRNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTNFFNASAAG ELN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGIELNHHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHL+GSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCKLPQ ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           GIDSNNQGDFNSADNKPACEFPVQDI+G EGLDSNAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIQGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNF+VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFAVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KAS+LMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 847

BLAST of CmaCh03G008180 vs. NCBI nr
Match: XP_022950404.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1670.2 bits (4324), Expect = 0.0e+00
Identity = 829/847 (97.87%), Postives = 836/847 (98.70%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTN FNASAAGSELN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHL+GSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCKLPQ ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           G+DSNNQGDFNSADNKPACEFPVQDIEG EGLDSNAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLF+DETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNF VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGS APCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KAS+LMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 847

BLAST of CmaCh03G008180 vs. NCBI nr
Match: XP_022950405.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1641.3 bits (4249), Expect = 0.0e+00
Identity = 819/847 (96.69%), Postives = 826/847 (97.52%), Query Frame = 0

Query: 55  MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 114
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 115 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 174
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 175 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 234
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 235 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 294
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 295 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 354
           CTSNLIVTNHEAQHFPGCRAHTN FNASAAGSELN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 355 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 414
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 415 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 474
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 475 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 534
           NQADKSHNSELRGNHL+GSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 535 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 594
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCKLPQ ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 595 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 654
           G+DSNNQGDFNSADNKPACEFPVQDIEG EGLDSNAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 655 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 714
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLF+DETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFRDETVIDSIKT 660

Query: 715 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 774
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNF VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFER 720

Query: 775 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 834
           QSSREGS APCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 835 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 894
           KAS+LMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837

Query: 895 NRLGNDV 902
           NRLGNDV
Sbjct: 841 NRLGNDV 837

BLAST of CmaCh03G008180 vs. TAIR 10
Match: AT2G22400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 897.1 bits (2317), Expect = 1.1e-260
Identity = 479/845 (56.69%), Postives = 590/845 (69.82%), Query Frame = 0

Query: 59  GRGG--RARTQRNHFRESRENVWKRSKPDS--NSANDTKDGNSNHWGPFQSQSHAFNEYY 118
           GRGG  R RTQR  F+ESRENVWKR K D+  + +++        W P  + +  F EYY
Sbjct: 2   GRGGRHRGRTQRKDFKESRENVWKRPKSDASVDGSDNAVPEQKPTWEPIVTVNPNFEEYY 61

Query: 119 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 178
           K+QGIV AEEWD F+E+LRK LPAAFR+NS+GQFC EI + L+NDFMKSLQ+E  +G E 
Sbjct: 62  KKQGIVKAEEWDLFMEILRKPLPAAFRVNSNGQFCDEIISILENDFMKSLQAEAIEGGEL 121

Query: 179 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 238
            AI+PL WYP NLAWHSNFSR ++RKNQ LERFHEFLKLENE+GNITRQEAVSMVPPLFL
Sbjct: 122 EAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLENEVGNITRQEAVSMVPPLFL 181

Query: 239 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 298
           DVHP+H+VLDMCAAPGSKTFQLLEIIH++S+PG LP GLVVAND D +R NLLIHQTKRM
Sbjct: 182 DVHPDHFVLDMCAAPGSKTFQLLEIIHEASEPGSLPNGLVVANDVDFKRSNLLIHQTKRM 241

Query: 299 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 358
           CTSNLIVTNHE Q FPGCR   N   AS  G   N    QL FDRVLCDVPCSGDGTLRK
Sbjct: 242 CTSNLIVTNHEGQQFPGCR--LNKSRASEKGISENMPINQLAFDRVLCDVPCSGDGTLRK 301

Query: 359 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 418
           APDIWR+WNSG+GNGLH LQ+ + MRG SLLKVGG+M+YSTCSMNPVE+EAVVAE+LR+ 
Sbjct: 302 APDIWRKWNSGMGNGLHSLQIILAMRGLSLLKVGGKMIYSTCSMNPVEDEAVVAEILRRC 361

Query: 419 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 478
             S+E++DVS++LP+L+RRPGLK WKVRD+G W  SYK V  +R+   + SMFP G+  K
Sbjct: 362 GDSVELLDVSDKLPELIRRPGLKAWKVRDKGGWFTSYKDVPQNRRGGVLVSMFPSGKYLK 421

Query: 479 NQADKSHNSELRGNHLNGSIG--SSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNS 538
           +  + +  +E     +NG        D+S     P  + +EV + P+ERCMRI+PHDQN+
Sbjct: 422 DSTETTEKNE--NGDVNGCEDGLKETDISVVDATPEEQAEEVSDLPLERCMRIIPHDQNT 481

Query: 539 GAFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPS 598
           GAFFIAVL+K +PLP   EKP  +  + +  KS     ++ +  + V V A V     P 
Sbjct: 482 GAFFIAVLQKKSPLPEFQEKP--NTKRNSTAKSTDSTEKSPSKESVVTVDAGV-----PD 541

Query: 599 EVGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEV 658
           E  ++   + D N              IE  + L+       +K+ +E +          
Sbjct: 542 ESAVEKVIEADSN--------------IEKNDSLEP------EKKITEGE---------- 601

Query: 659 NVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSI 718
                 IT            +D E +   +  KRK+ +QGKWKG DPVV  KDETVI+ I
Sbjct: 602 -----SIT------------EDKEANSSNAGGKRKVPMQGKWKGFDPVVFVKDETVINGI 661

Query: 719 KTFYGI-NEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKM 778
           K FYGI +E+FPL GHLV RNTDT+ VKRIYYVSKSVK+VL+LNF+VGQQLKI S+GLKM
Sbjct: 662 KEFYGIKDESFPLHGHLVARNTDTSSVKRIYYVSKSVKEVLQLNFAVGQQLKIASVGLKM 721

Query: 779 FERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAA 838
           FERQS++EGSS  C FRI+SEGLPVILPYITKQ+L     DFK L+Q KSIK+ DF +  
Sbjct: 722 FERQSAKEGSSTLCPFRISSEGLPVILPYITKQVLYTPMADFKLLLQDKSIKFHDFVNPQ 781

Query: 839 FGEKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLE 897
             +KA+ L++G CV++L  G       +K D STIAI CW+G++SL+VM T  DCQELLE
Sbjct: 782 LAQKATDLVMGSCVVILSDG----EVPVKADASTIAISCWRGKASLAVMATVADCQELLE 784

BLAST of CmaCh03G008180 vs. TAIR 10
Match: AT4G40000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 821.6 bits (2121), Expect = 6.1e-238
Identity = 456/848 (53.77%), Postives = 572/848 (67.45%), Query Frame = 0

Query: 57  GKGRGGRART-QRNHFRESR-ENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 116
           G+GR  R  T +RN    S  +NV K  K +++        ++N   P  S  H F EYY
Sbjct: 2   GRGRRHRGSTSKRNQIENSNDDNVRKPLKSEASF------DSNNLQKPICSNLH-FEEYY 61

Query: 117 KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 176
           KEQ IV A+EWD+F+E LRK LPAAFR+ S+GQFC EIR +L+NDFM SLQ+E  +  E 
Sbjct: 62  KEQRIVKAQEWDSFMEFLRKPLPAAFRVYSNGQFCDEIRLKLENDFMISLQAEAIESGEL 121

Query: 177 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 236
            AI+PL WYP NLAWHSNFSR ++RKNQ LERFHEFLKLE E GN+TRQE+VSMVPPLFL
Sbjct: 122 EAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLETEAGNMTRQESVSMVPPLFL 181

Query: 237 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 296
           DVHP+H+VLDMCAAPGSKTFQLLEIIH+SS+PG LP G+VVAND D +R NLLIHQTKR 
Sbjct: 182 DVHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVANDVDYKRSNLLIHQTKRT 241

Query: 297 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHN-TQLTFDRVLCDVPCSGDGTLR 356
           CT+NL+VTN+E QHFP C    N     +  SE N H   QL FDRVLCDVPCSGDGTLR
Sbjct: 242 CTTNLMVTNNEGQHFPSC----NTKRTLSVASETNPHPIDQLLFDRVLCDVPCSGDGTLR 301

Query: 357 KAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRK 416
           KAPDIWRRWNSG GNGLH LQV + MRG SLLKVGGRMVYSTCSMNP+E+EAVVAE+LR+
Sbjct: 302 KAPDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEILRR 361

Query: 417 SEGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGR 476
              S+E+VDVS++LP+L+RRPGL KWKV DRG W  SYK V   ++   + SMFP G+  
Sbjct: 362 CGCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSGKSD 421

Query: 477 KNQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSG 536
           K+               +G   S  +++  + D     +EVC+ P+E CMRI+PHDQN+G
Sbjct: 422 KDS--------------SGGKNSYEEMASISSD--ESAEEVCDLPLELCMRILPHDQNTG 481

Query: 537 AFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSE 596
            FFIAVL KV+PLP   EK +                +N++   N++ S      E+ S+
Sbjct: 482 GFFIAVLHKVSPLPDFQEKVTQ--------------KRNLSTRNNINSSEKSSSYEAVSD 541

Query: 597 VGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVN 656
             +    +G                                     E  LE  + +N   
Sbjct: 542 TVVTKPEEGT-----------------------------------EEIVLEATVSENGFE 601

Query: 657 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKL-QIQGKWKGVDPVVLFKDETVIDSI 716
              +E T  E  +E +++ DD E        KR++  +QGKWKG+ PVV  +D+T I+ I
Sbjct: 602 PPEMESTWNEGIVELAQKLDDIE-------GKREVPSMQGKWKGLYPVVFLRDQTGINGI 661

Query: 717 KTFYGI-NEAFPLFGHLVTRNTDTN---HVKRIYYVSKSVKDVLELNFSVGQQLKITSIG 776
           KTFYGI +E+FPL+GHLVTRN+D +   +VKRIYYVSK+VK+VLELNF+VG+ LKI+S+G
Sbjct: 662 KTFYGIKDESFPLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLKISSVG 721

Query: 777 LKMFERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFA 836
           LKMFE+QS++E  +  CSFRITSEGLPVILPY+TKQIL  +  DFK+L+QHKSIK+ DF 
Sbjct: 722 LKMFEKQSAKECEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIKFLDFI 766

Query: 837 DAAFGEKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQE 896
               GEKA+ L LG CV+VL       S+  KV+ STIAIGCWKG++SL+VMVT +DC +
Sbjct: 782 HPQIGEKAAELALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTTVDCHQ 766

BLAST of CmaCh03G008180 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 2.6e-15
Identity = 62/200 (31.00%), Postives = 87/200 (43.50%), Query Frame = 0

Query: 223 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQ 282
           Q A S +P + L    N  ++D+ AAPG KT  +  ++  +        GL+ AN+  V 
Sbjct: 344 QGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNT--------GLIYANEMKVP 403

Query: 283 RCNLLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLC 342
           R   L     RM  +N IV N++                   G EL     Q T DRVL 
Sbjct: 404 RLKSLTANLHRMGVTNTIVCNYD-------------------GRELPKVLGQNTVDRVLL 463

Query: 343 DVPCSGDGTLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 402
           D PCSG G + K   +              LQ Q+ +    ++    K GG +VYSTCS+
Sbjct: 464 DAPCSGTGIISKDESVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSI 516

Query: 403 NPVENEAVVAELLRKSEGSL 419
              ENEAV+   L+K +  L
Sbjct: 524 MVTENEAVIDYALKKRDVKL 516

BLAST of CmaCh03G008180 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 1.3e-14
Identity = 61/200 (30.50%), Postives = 87/200 (43.50%), Query Frame = 0

Query: 223 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQ 282
           Q A S +P + L       V+DM AAPG KT  +  ++  +        G++ AN+  V 
Sbjct: 327 QSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNT--------GIIYANEMKVP 386

Query: 283 RCNLLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLC 342
           R   L     RM  +N IV N++                   G EL     Q + DRVL 
Sbjct: 387 RLKSLSANLHRMGVTNTIVCNYD-------------------GRELTKVLGQSSVDRVLL 446

Query: 343 DVPCSGDGTLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 402
           D PCSG G + K   +    ++        LQ Q+ +    L+    K GG +VYSTCS+
Sbjct: 447 DAPCSGTGVISKDESVKTSKSADDIKKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSV 499

Query: 403 NPVENEAVVAELLRKSEGSL 419
              ENEAV+   L+  +  L
Sbjct: 507 MIPENEAVIDYALKNRDVKL 499

BLAST of CmaCh03G008180 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 78.6 bits (192), Expect = 2.9e-14
Identity = 62/199 (31.16%), Postives = 91/199 (45.73%), Query Frame = 0

Query: 226 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCN 285
           VS+V P      P   ++D CAAPG KT  +   +           G++ A D +  R  
Sbjct: 323 VSVVKP-----QPGERIMDACAAPGGKTLFMASCLKGQ--------GMIYAMDVNEGRLR 382

Query: 286 LLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVP 345
           +L    K      LI T H                   +   + +   ++ +D+VL D P
Sbjct: 383 ILGETAKSHQVDGLITTIH-------------------SDLRVFAETNEVQYDKVLLDAP 442

Query: 346 CSGDGTLRKAPDIWRRWNSGVGNGLH--GLQVQIGMRGASLLKVGGRMVYSTCSMNPVEN 405
           CSG G L K  D+  RWN  + + L    LQ ++    + L+K GG +VYSTCS++P EN
Sbjct: 443 CSGLGVLSKRADL--RWNRKLEDMLELTKLQDELLDSASKLVKHGGVLVYSTCSIDPEEN 487

Query: 406 EA-VVAELLRKSEGSLEVV 422
           E  V A LLR  E +++ V
Sbjct: 503 EGRVEAFLLRHPEFTIDPV 487