CmaCh03G007260 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G007260
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	abscisic acid 8'-hydroxylase 3-like
Location	Cma_Chr03: 5830083 .. 5832977 (+)
RNA-Seq Expression	CmaCh03G007260
Synteny	CmaCh03G007260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGTGGAAGGAGGAGCTTGTGCTCTTAGCTCAAAACTATTTTGACACTACGACAGTGGCGGCAGTTCTTTCCGTAGGTATTTTAGTGTTGGTTTCGAAAGTTTGCTGGAGACAGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTACCCTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCTAATAACAGCACCAGAGGGTGTTACCAATTTGTCAGGCTCCGCCGGTTGTGGTTAGTGGTTCTGTCCCCATGGTGATTATGGATATTCTTTGTTAGTCTGACTTCTAATATTCCTTTAATATGATTCCAATTTTCCTCATTCAAGGCCAAGTTTGAACTATTTCCCTAAAATTTGTAATCTTTTACGTGATTCCTTTAGCTTCTAATTTGATAATTTTAGAACTTTTGATACAAAACTAGACTTGGTTACCGTCCCATACGAGTCAACTCCATTGAATTTTTACATTTGACGACTGATGGGAGTTCCATGTATACATATGTATGCATTAGCATATACATCCCGGGCAGTTTAGAACATGGAACTGATGGCTGTGTGAAACTAAACCAGGCATGGTAGGTGGTTCAGGACAAGGCTATTTGGAAAAATCCATGTGTTTGTTCCAAGTGCAGAAGGTGCAAAGGCAATTCTTGCCAATGATTTTGTCCAATTCAACAAGGGATACGTGAAATCCATGGGCGATGCTGTTGGAGAGAAGAGTTTACTATGCGTTTCCCATGAGGACCACAGAAGGATTAGGCGTCTTCTGGCTGATCCTTTCTCTATGACTTCTCTGTCTAAGTTTGTCAAACAATTTGACGACATGCTCTCCAAAAGGCTACAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTGCTTGATTTCAGCATGAAGGTTATAGAAAATAAAAATATAGTCATCTTTCTCAAACATGTCTGTTTGACTTGGCAGCCAACATATCTTACGTTATGTGATTCGCAGATAACATTCGATTCCATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGATTGAAAAAGACTGTACAGCAGTCACTGACGCCATGCTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGTACATTGATACACTTCAACTAGGACTTTCTAGCTTCCTTCAACTTTAGAAGCATATAAACTCGTCATCAACAAAATGTGCAGGCACGAAACCGACTAATGGAAAGGTTAGGAGAAATAATATCCAGGCGAAGGAGGGGAGAGGAGTCACCAGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCGTATCCTCCGGATCAGAAGTTAGATGACTCAGAGATAATGGATAACCTTTTGACACTGATAATTGCAAGCCAAACTACCACAGCTGCTGCTATGATGTGGAGTGTCAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGGCTGCGGGTAAGATCCATAACCTGTTAAGTTATCCAAAGAAAAGTTAACAAAGGTTACCCATAATCCACTGCTCTTCATACTTTCATGCTGAGTACTCCTGTGGAATGAATACTAATGCCCATTGCAACTCTCCAGGAGGAACAGTTGTCAATTCTACGAAAGAAGGAAGATGGAGAACTACTTACTCTTGAAGATCTTAATAAAATGTCCTTTGGTTCAAAGGTTATAATAAGCAGCCTAAGTTTGTTTTTATTTTGTAAGAAGTGTACAGAGTGCTTCAGATTGTAAATAATTCCATGCACAATGTACTAACAGGCCGCTGACAACTTAATTCATATGCATTGCAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGTGTGGCACTAGGGGATTGCAGATTAGAAGGTACGTTCCTCCTGTTACATTCATTCTCTTTTTGATATTTCACTAATGGAAACAAATGAACAAGATTTTCTATTCTACCGTAGGAAATGAAGATGACTCCTGTAGAACTATTTCTCTGCTAGAAATATACACATACGTATACAGAAAAGAATTATATTCTATTTGTAAATCGATAGACAGGATGATAAAGAGTTTTTGAACAAATTGGGACCCCCTCCAACGCATTTTGAACGTGGTATGCAGGTTTTGAAATAAAGAAAGGATGGCATGTGAACATCGATGCAACATGCATTCATTATGACCCAGATTTGTACAAGGACCCCATGCAATTCAACCCTTCGAGATTTGATGTATCTACCTACAATCAATCACTGTTTCTTCTTGTCCCNCCCCCCCCCTCTCTTCAGCTACTAAACCTATATTGTTCATGCACCTACGGGGCATAACTTCAGGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGTCTCGGAATCAACATGGCCAAATTAACAATGTTGGTCTTTTTGCACAGGATGACTGGAGGGTACAGGTGAGTCTGTACATTCCTAAAGCAGCAGGCATTTCATCCCGTTCCAAGAACTAATTCCGAACTTCCTCTTCTTATCCTTATCATTATTTTGCCTTTGTATTGTTTAGGTGGACGGTTGATGATCCAGATCCTTGCCTCGAAAAGAAGGCACATATCCCTAGGCTGAGAAGCGGCTGCCCCATTACTTTGAAGACCTTGTACGATGGGACATAAGCATAGATGTGAGAAATCTCAGGTACAGGTAGGAGAAGTGGCAAGATTCTAATGCATGGTAATGAGGAAGCAGCTGGATAATATGTATCATCATGGCCTCTAATTACCCACTTTTTTTATGCTTCGCTTAAAAGTTAACCAGAAGAGAAAAAATAAAATGAAAAAGTGAACTTTGCAACATTGCAGTGGGAACTCCTCACTTTATGATGTCC

mRNA sequence

ATGTTGTGGAAGGAGGAGCTTGTGCTCTTAGCTCAAAACTATTTTGACACTACGACAGTGGCGGCAGTTCTTTCCGTAGGTATTTTAGTGTTGGTTTCGAAAGTTTGCTGGAGACAGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTACCCTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCTAATAACAGCACCAGAGGGTGTTACCAATTTGTCAGGCTCCGCCGGCATGGTAGGTGGTTCAGGACAAGGCTATTTGGAAAAATCCATGTGTTTGTTCCAAGTGCAGAAGGTGCAAAGGCAATTCTTGCCAATGATTTTGTCCAATTCAACAAGGGATACGTGAAATCCATGGGCGATGCTGTTGGAGAGAAGAGTTTACTATGCGTTTCCCATGAGGACCACAGAAGGATTAGGCGTCTTCTGGCTGATCCTTTCTCTATGACTTCTCTGTCTAAGTTTGTCAAACAATTTGACGACATGCTCTCCAAAAGGCTACAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTGCTTGATTTCAGCATGAAGATAACATTCGATTCCATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGATTGAAAAAGACTGTACAGCAGTCACTGACGCCATGCTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAAACCGACTAATGGAAAGGTTAGGAGAAATAATATCCAGGCGAAGGAGGGGAGAGGAGTCACCAGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCGTATCCTCCGGATCAGAAGTTAGATGACTCAGAGATAATGGATAACCTTTTGACACTGATAATTGCAAGCCAAACTACCACAGCTGCTGCTATGATGTGGAGTGTCAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGGCTGCGGGAGGAACAGTTGTCAATTCTACGAAAGAAGGAAGATGGAGAACTACTTACTCTTGAAGATCTTAATAAAATGTCCTTTGGTTCAAAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGTGTGGCACTAGGGGATTGCAGATTAGAAGGTTTTGAAATAAAGAAAGGATGGCATGTGAACATCGATGCAACATGCATTCATTATGACCCAGATTTGTACAAGGACCCCATGCAATTCAACCCTTCGAGATTTGATGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGTCTCGGAATCAACATGGCCAAATTAACAATGTTGGTCTTTTTGCACAGGATGACTGGAGGGTACAGGTGGACGGTTGATGATCCAGATCCTTGCCTCGAAAAGAAGGCACATATCCCTAGGCTGAGAAGCGGCTGCCCCATTACTTTGAAGACCTTGTACGATGGGACATAAGCATAGATGTGAGAAATCTCAGGTACAGGTAGGAGAAGTGGCAAGATTCTAATGCATGGTAATGAGGAAGCAGCTGGATAATATGTATCATCATGGCCTCTAATTACCCACTTTTTTTATGCTTCGCTTAAAAGTTAACCAGAAGAGAAAAAATAAAATGAAAAAGTGAACTTTGCAACATTGCAGTGGGAACTCCTCACTTTATGATGTCC

Coding sequence (CDS)

ATGTTGTGGAAGGAGGAGCTTGTGCTCTTAGCTCAAAACTATTTTGACACTACGACAGTGGCGGCAGTTCTTTCCGTAGGTATTTTAGTGTTGGTTTCGAAAGTTTGCTGGAGACAGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTACCCTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCTAATAACAGCACCAGAGGGTGTTACCAATTTGTCAGGCTCCGCCGGCATGGTAGGTGGTTCAGGACAAGGCTATTTGGAAAAATCCATGTGTTTGTTCCAAGTGCAGAAGGTGCAAAGGCAATTCTTGCCAATGATTTTGTCCAATTCAACAAGGGATACGTGAAATCCATGGGCGATGCTGTTGGAGAGAAGAGTTTACTATGCGTTTCCCATGAGGACCACAGAAGGATTAGGCGTCTTCTGGCTGATCCTTTCTCTATGACTTCTCTGTCTAAGTTTGTCAAACAATTTGACGACATGCTCTCCAAAAGGCTACAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTGCTTGATTTCAGCATGAAGATAACATTCGATTCCATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGATTGAAAAAGACTGTACAGCAGTCACTGACGCCATGCTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAAACCGACTAATGGAAAGGTTAGGAGAAATAATATCCAGGCGAAGGAGGGGAGAGGAGTCACCAGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCGTATCCTCCGGATCAGAAGTTAGATGACTCAGAGATAATGGATAACCTTTTGACACTGATAATTGCAAGCCAAACTACCACAGCTGCTGCTATGATGTGGAGTGTCAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGGCTGCGGGAGGAACAGTTGTCAATTCTACGAAAGAAGGAAGATGGAGAACTACTTACTCTTGAAGATCTTAATAAAATGTCCTTTGGTTCAAAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGTGTGGCACTAGGGGATTGCAGATTAGAAGGTTTTGAAATAAAGAAAGGATGGCATGTGAACATCGATGCAACATGCATTCATTATGACCCAGATTTGTACAAGGACCCCATGCAATTCAACCCTTCGAGATTTGATGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGTCTCGGAATCAACATGGCCAAATTAACAATGTTGGTCTTTTTGCACAGGATGACTGGAGGGTACAGGTGGACGGTTGATGATCCAGATCCTTGCCTCGAAAAGAAGGCACATATCCCTAGGCTGAGAAGCGGCTGCCCCATTACTTTGAAGACCTTGTACGATGGGACATAA

Protein sequence

MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETFSFLSANNSTRGCYQFVRLRRHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGYVKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTLYDGT

Homology

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BLAST of CmaCh03G007260 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.5e-57
Identity = 145/465 (31.18%), Postives = 241/465 (51.83%), Query Frame = 0


Query: 18  TTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETFSFLSANNSTRGCYQFVR 77
           T  AA L + +L  ++ V  R  S  +   PG +G P+VGETF   S + +    +   +
Sbjct: 9   TLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV---FFAAK 68

Query: 78  LRRHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGYVKSMGDAVGEKSLLCVSHE 137
            RR+G  F+T + G   V + S E AK +L      F   +  S    +G++++     +
Sbjct: 69  QRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGD 128

Query: 138 DHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGKSFVLLDFSMKITFDSMCNM 197
            H ++R+L+   F   ++   V   + +  + L     DG            TF+     
Sbjct: 129 YHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVALIS 188

Query: 198 LMSLRDESTLRQIEKDCTAVTD-AMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRR 257
           ++  +DE   R+  K C  + +    S P  +PGT ++K +KAR  L + L  I+S+RR+
Sbjct: 189 ILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQ 248

Query: 258 GEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENRE 317
              S  D L S ++  +      L D +I DN++ +I A++ TTA+ + W +K+L +N  
Sbjct: 249 NPSSHTDLLGSFMEDKA-----GLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPT 308

Query: 318 AQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRL 377
             E + EEQ++I + K++GE LT ED  KM    +V++ETLR + +L +  R A+ D   
Sbjct: 309 VLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEY 368

Query: 378 EGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINM 437
           EG+ I KGW V      IH++ D++ DP +F+PSRF+   KP +F+PFGSG  +C G  +
Sbjct: 369 EGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNEL 428

Query: 438 AKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPITLK 482
           AKL + V +H +T  YRW++  P   ++        ++G PI L+
Sbjct: 429 AKLEISVLIHHLTTKYRWSIVGPSDGIQ-YGPFALPQNGLPIALE 460

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BLAST of CmaCh03G007260 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.1e-55
Identity = 155/496 (31.25%), Postives = 240/496 (48.39%), Query Frame = 0


Query: 1   MLWKEELVLL--AQNYFDTTTVAAVLSVGI--LVLVSKVCWRQRSKNVGGI-PGHLGLPF 60
           M+WKE + +L  AQ   +   +  V       L     +    R+K   GI PG  G P 
Sbjct: 1   MVWKEAVSVLQKAQELKEPPLMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPL 60

Query: 61  VGETFSFLSANNSTRGCYQF-VRLRRHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQF 120
           +GET  FL           F  R +++G  F T L G   V   S EG + + +N+    
Sbjct: 61  IGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNE---- 120

Query: 121 NKGYVKSMGDAVG---EKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQ 180
           NK  V S   +VG     SL+    +D +R+RR+L       +L +FV + D M  + L 
Sbjct: 121 NKLVVNSWPSSVGNLFRSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLA 180

Query: 181 KLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGT 240
           +        + L    + TF   C++  S+  +  L ++          ++  P  +PGT
Sbjct: 181 EHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGT 240

Query: 241 RYYKGIKARNRLMERLGEIISRRR----RGEESPEDFLQSMLQRDSYPPDQKLDDSEIMD 300
           RY K   A N + ++LG II+ R+     G  SPE  L S L  +     + L D+EI D
Sbjct: 241 RYNKTKHAANAIRQQLGSIINERKIALEAGNASPEQDLLSFLLSNVDEQGESLTDNEIQD 300

Query: 301 NLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMS 360
           N+L L+ A   T+++ +   +KFL EN    E +  EQL+I   KE+G+LL  EDL +M 
Sbjct: 301 NILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMK 360

Query: 361 FGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQF 420
           +  +V +E LR+   +    R A+ +   +GF I KGW ++      H   + + +P +F
Sbjct: 361 YSWRVAQEALRLFPAVQGSFRKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKF 420

Query: 421 NPSRFD-EIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK 480
           +PSRF+ E   PY+F+PFG GPR C G   A++ +L+FLH +   + W + +P   LEK 
Sbjct: 421 DPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARMEILIFLHNIVKNFNWNLVNP---LEKV 480

[Close]

BLAST of CmaCh03G007260 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 5.2e-55
Identity = 131/410 (31.95%), Postives = 216/410 (52.68%), Query Frame = 0


Query: 48  PGHLGLPFVGETFSFLSANNSTRGCYQFVRLRRHGRWFRTRLFGKIHVFVPSAEGAKAIL 107
           PG +G P+VGETF   S + +    +   + +R+G  F+T + G   V + S E AK +L
Sbjct: 38  PGTMGWPYVGETFQLYSQDPNV---FFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVL 97

Query: 108 ANDFVQFNKGYVKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLS 167
                 F   +  S    +G++++     + H ++R+L+   F   S+   V   + +  
Sbjct: 98  VTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQ 157

Query: 168 KRLQKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTD-AMLSFPF 227
             L+    +G            TF+     +   +DE   R+  K C  + +    S P 
Sbjct: 158 DSLRSW--EGTMINTYQEMKTYTFNVALLSIFG-KDEVLYREDLKRCYYILEKGYNSMPV 217

Query: 228 MIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIM 287
            +PGT ++K +KAR  L + L  I+S RR+   S  D L S +        ++L D +I 
Sbjct: 218 NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM-----GDKEELTDEQIA 277

Query: 288 DNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKM 347
           DN++ +I A++ TTA+ M W +K+L EN    E + EEQ++I + KE+GE LT  D  KM
Sbjct: 278 DNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKM 337

Query: 348 SFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQ 407
              S+V++ETLR++++L +  R A+ D   EG+ I KGW V      IH+  D++ +P +
Sbjct: 338 PLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGK 397

Query: 408 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTV 457
           F+PSRF+   KP +F+PFG+G  +C G  +AKL M + +H +T  Y W++
Sbjct: 398 FDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSI 436

[Close]

BLAST of CmaCh03G007260 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.0e-54
Identity = 129/411 (31.39%), Postives = 208/411 (50.61%), Query Frame = 0


Query: 48  PGHLGLPFVGETFSFLSANNSTRGCYQFVRLRRHGRWFRTRLFGKIHVFVPSAEGAKAIL 107
           PG +G P++GET    S + +    +   + +R+G  F+T + G   V + S E A+ +L
Sbjct: 46  PGSMGWPYIGETLQLYSQDPNV---FFASKQKRYGEIFKTHILGCPCVMLASPEAARFVL 105

Query: 108 ANDFVQFNKGYVKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLS 167
                 F   Y +S    +G  +L     + H R+R+L+  P    +L   V   +  + 
Sbjct: 106 VTQAHLFKPTYPRSKERMIGPSALFFNQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVR 165

Query: 168 KRLQKLVKDGKSFVLLDFSMKITFD-SMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPF 227
             L     DG          +++FD  +  +     DE    ++ ++   V     SFP 
Sbjct: 166 STLASW--DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPN 225

Query: 228 MIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIM 287
             PGT YYK I+AR RL   L +I+  RR   E   D L  ++Q  +      L D ++ 
Sbjct: 226 SFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVA 285

Query: 288 DNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGEL-LTLEDLNK 347
           DN++ ++ A+Q TTA+ + W VK+L ++ +  E +R EQ +I    + G L LT      
Sbjct: 286 DNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRS 345

Query: 348 MSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPM 407
           M+   KV+ E+LRM++++ +  R A+ D   +GF I KGW V      IH++PD ++DP 
Sbjct: 346 MALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQ 405

Query: 408 QFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTV 457
           +F+PSRF    +P +F+PFG+G   C G  +AKL MLV +H +  GYRW +
Sbjct: 406 KFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEI 451

[Close]

BLAST of CmaCh03G007260 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.0e-54
Identity = 129/411 (31.39%), Postives = 208/411 (50.61%), Query Frame = 0


Query: 48  PGHLGLPFVGETFSFLSANNSTRGCYQFVRLRRHGRWFRTRLFGKIHVFVPSAEGAKAIL 107
           PG +G P++GET    S + +    +   + +R+G  F+T + G   V + S E A+ +L
Sbjct: 46  PGSMGWPYIGETLQLYSQDPNV---FFASKQKRYGEIFKTHILGCPCVMLASPEAARFVL 105

Query: 108 ANDFVQFNKGYVKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLS 167
                 F   Y +S    +G  +L     + H R+R+L+  P    +L   V   +  + 
Sbjct: 106 VTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVR 165

Query: 168 KRLQKLVKDGKSFVLLDFSMKITFD-SMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPF 227
             L     DG          +++FD  +  +     DE    ++ ++   V     SFP 
Sbjct: 166 STLASW--DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPN 225

Query: 228 MIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIM 287
             PGT YYK I+AR RL   L +I+  RR   E   D L  ++Q  +      L D ++ 
Sbjct: 226 SFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVA 285

Query: 288 DNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGEL-LTLEDLNK 347
           DN++ ++ A+Q TTA+ + W VK+L ++ +  E +R EQ +I    + G L LT      
Sbjct: 286 DNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRS 345

Query: 348 MSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPM 407
           M+   KV+ E+LRM++++ +  R A+ D   +GF I KGW V      IH++PD ++DP 
Sbjct: 346 MALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQ 405

Query: 408 QFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTV 457
           +F+PSRF    +P +F+PFG+G   C G  +AKL MLV +H +  GYRW +
Sbjct: 406 KFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEI 451

[Close]

BLAST of CmaCh03G007260 vs. ExPASy TrEMBL
Match: A0A6J1IMR3 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478438 PE=3 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 9.9e-283
Identity = 487/489 (99.59%), Postives = 487/489 (99.59%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300
           ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360
           TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480
           YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

Query: 481 TLKTLYDGT 488
           TLKTLYDGT
Sbjct: 481 TLKTLYDGT 489

[Close]

BLAST of CmaCh03G007260 vs. ExPASy TrEMBL
Match: A0A6J1IQ66 (abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478438 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.3e-278
Identity = 487/518 (94.02%), Postives = 487/518 (94.02%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMK-----------------------------ITFDSMCNMLMSLRDESTLRQ 240
           SFVLLDFSMK                             ITFDSMCNMLMSLRDESTLRQ
Sbjct: 181 SFVLLDFSMKVIENKNIVIFLKHVCLTWQPTYLTLCDSQITFDSMCNMLMSLRDESTLRQ 240

Query: 241 IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML 300
           IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML
Sbjct: 241 IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML 300

Query: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360
           QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL
Sbjct: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360

Query: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI 420
           RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI
Sbjct: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI 420

Query: 421 DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT 480
           DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT
Sbjct: 421 DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT 480

Query: 481 GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTLYDGT 488
           GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTLYDGT
Sbjct: 481 GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTLYDGT 518

[Close]

BLAST of CmaCh03G007260 vs. ExPASy TrEMBL
Match: A0A6J1GG59 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453639 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.1e-277
Identity = 477/485 (98.35%), Postives = 481/485 (99.18%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLS+GILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDC+AV+DAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300
           ARNRLMERL EIISRRR+GEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360
           TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480
           YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

Query: 481 TLKTL 484
           TLKTL
Sbjct: 481 TLKTL 485

[Close]

BLAST of CmaCh03G007260 vs. ExPASy TrEMBL
Match: A0A6J1GG87 (abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453639 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.5e-272
Identity = 477/514 (92.80%), Postives = 481/514 (93.58%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLS+GILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMK-----------------------------ITFDSMCNMLMSLRDESTLRQ 240
           SFVLLDFSMK                             ITFDSMCNMLMSLRDESTLRQ
Sbjct: 181 SFVLLDFSMKVIENKNIVIFLKHVCLTWQPTYLTLCDLQITFDSMCNMLMSLRDESTLRQ 240

Query: 241 IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML 300
           IEKDC+AV+DAMLS PFMIPGTRYYKGIKARNRLMERL EIISRRR+GEESPEDFLQSML
Sbjct: 241 IEKDCSAVSDAMLSLPFMIPGTRYYKGIKARNRLMERLREIISRRRKGEESPEDFLQSML 300

Query: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360
           QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL
Sbjct: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360

Query: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI 420
           RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI
Sbjct: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI 420

Query: 421 DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT 480
           DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT
Sbjct: 421 DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT 480

Query: 481 GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTL 484
           GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTL
Sbjct: 481 GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTL 514

[Close]

BLAST of CmaCh03G007260 vs. ExPASy TrEMBL
Match: A0A5A7SHU1 (Abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001790 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.1e-252
Identity = 437/485 (90.10%), Postives = 458/485 (94.43%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           M+  E L  +AQ YF+  TVAA+LS+ ILVLVSKV WR RSKN G IPGHLGLPFVGETF
Sbjct: 1   MVCTEGLAFIAQIYFN-PTVAALLSIAILVLVSKVWWRLRSKNDGEIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCY FVRLRR  HGRWF+TRLFGKIHVFVPSAEGAKAI  NDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFTNDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHE+HRRIRRLL+DPFSMTSLSKFVK+FDDMLSKRL+KL KDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGK 180

Query: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFDSMCNMLMSL+DESTLRQIEKDC AV+DAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLKDESTLRQIEKDCKAVSDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300
           ARNRLME+  EIISRRRRGEESPEDFL+SMLQRDSYP ++KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMEKFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQT 300

Query: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSIL+KKEDGELL LEDLNKMS+GSKVVKETLR
Sbjct: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLCLEDLNKMSYGSKVVKETLR 360

Query: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPRVALGDCRLE FEIKKGWHVNIDATCIHYDPD+YKDPM+FNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMEFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480
           YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK HIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKTHIPRLRSGCPI 480

Query: 481 TLKTL 484
           TLKTL
Sbjct: 481 TLKTL 484

[Close]

BLAST of CmaCh03G007260 vs. NCBI nr
Match: XP_022978466.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 981.9 bits (2537), Expect = 2.0e-282
Identity = 487/489 (99.59%), Postives = 487/489 (99.59%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300
           ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360
           TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480
           YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

Query: 481 TLKTLYDGT 488
           TLKTLYDGT
Sbjct: 481 TLKTLYDGT 489

[Close]

BLAST of CmaCh03G007260 vs. NCBI nr
Match: XP_023543351.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968.8 bits (2503), Expect = 1.8e-278
Identity = 481/489 (98.36%), Postives = 483/489 (98.77%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLS+GILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAV+DAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300
           ARNRLMERL EIISRRR GEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT
Sbjct: 241 ARNRLMERLREIISRRRSGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360
           TTAAAM WSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR
Sbjct: 301 TTAAAMTWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480
           YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

Query: 481 TLKTLYDGT 488
           TLKTL DGT
Sbjct: 481 TLKTLKDGT 489

[Close]

BLAST of CmaCh03G007260 vs. NCBI nr
Match: XP_022978465.1 (abscisic acid 8'-hydroxylase 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 966.5 bits (2497), Expect = 8.9e-278
Identity = 487/518 (94.02%), Postives = 487/518 (94.02%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMK-----------------------------ITFDSMCNMLMSLRDESTLRQ 240
           SFVLLDFSMK                             ITFDSMCNMLMSLRDESTLRQ
Sbjct: 181 SFVLLDFSMKVIENKNIVIFLKHVCLTWQPTYLTLCDSQITFDSMCNMLMSLRDESTLRQ 240

Query: 241 IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML 300
           IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML
Sbjct: 241 IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML 300

Query: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360
           QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL
Sbjct: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360

Query: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI 420
           RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI
Sbjct: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNI 420

Query: 421 DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT 480
           DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT
Sbjct: 421 DATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMT 480

Query: 481 GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTLYDGT 488
           GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTLYDGT
Sbjct: 481 GGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTLYDGT 518

[Close]

BLAST of CmaCh03G007260 vs. NCBI nr
Match: XP_022950585.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita moschata] >KAG7034125.1 Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 962.2 bits (2486), Expect = 1.7e-276
Identity = 477/485 (98.35%), Postives = 481/485 (99.18%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLS+GILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDC+AV+DAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300
           ARNRLMERL EIISRRR+GEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360
           TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480
           YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

Query: 481 TLKTL 484
           TLKTL
Sbjct: 481 TLKTL 485

[Close]

BLAST of CmaCh03G007260 vs. NCBI nr
Match: KAG6603947.1 (Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958.0 bits (2475), Expect = 3.2e-275
Identity = 476/485 (98.14%), Postives = 480/485 (98.97%), Query Frame = 0


Query: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELVLLAQNYFDTTTVAAVLS+GILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTRGCYQFVRLRR--HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120
           SFLSANNSTRGCYQFVRLRR  HGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180
           VKSMGDAVGEKSLLCVS EDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSLEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDC+AV+DAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300
           ARNRLMERL EIISRRR+GEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360
           TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480
           YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

Query: 481 TLKTL 484
           TLKTL
Sbjct: 481 TLKTL 485

[Close]

BLAST of CmaCh03G007260 vs. TAIR 10
Match: AT1G19630.1 (cytochrome P450, family 722, subfamily A, polypeptide 1 )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 217/465 (46.67%), Postives = 302/465 (64.95%), Query Frame = 0


Query: 21  AAVLSVGILVLVSKVCWRQRSKNVGGI-PGHLGLPFVGETFSFLSANNSTRGCYQFVRLR 80
           AA+L++G  +   K+ ++ R K+  G+ PG  G P +GET  F+ + NS +G Y+FVR R
Sbjct: 12  AAMLTLGKFL---KIMFQDRKKSTAGVPPGSDGFPVIGETLQFMLSVNSGKGFYEFVRSR 71

Query: 81  --RHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGYVKSMGDAVGEKSLLCVSHE 140
             R+G  FRT LFG+ HVF+ + E A+ +L ND   F K Y+KS+G+ VG++SLLC    
Sbjct: 72  RIRYGSCFRTSLFGETHVFLSTTESARLVLNNDSGMFTKRYIKSIGELVGDRSLLCAPQH 131

Query: 141 DHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGKSFVLLDFSMKITFDSMCNM 200
            H+ +R  L + FS  S +  V+ FD+++   L      G + VLL   ++ITF +MC M
Sbjct: 132 HHKILRSRLINLFSKRSTALMVRHFDELVVDALGGWEHRG-TVVLLTDLLQITFKAMCKM 191

Query: 201 LMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRG 260
           L+SL  E  L  +++D   V +AML+FP  +P TR++KGI AR R+ME L +II  RR  
Sbjct: 192 LVSLEKEEELGSMQRDVGFVCEAMLAFPLNLPWTRFHKGIMARGRVMEMLEKIIRERRNE 251

Query: 261 EES----PEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDE 320
             S     EDFLQ +L  D+  P  +L D+EI DN+LT+IIA Q TTA+A+ W VK+L E
Sbjct: 252 INSHNNHHEDFLQQLLAVDNDTP--QLTDAEIKDNILTMIIAGQDTTASALTWMVKYLGE 311

Query: 321 NREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGD 380
           N++  + L EEQ  I +K  +   L LEDL++M + SK+VKE+LRM++V+ WFPR+ L D
Sbjct: 312 NQKVLDILIEEQSQITKKASNKPFLELEDLSEMPYASKMVKESLRMASVVPWFPRLVLQD 371

Query: 381 CRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLG 440
           C +EG++IKKGW++NIDA  IH DP +Y +P +FNP RF+E  K  SF+ FG G RTCLG
Sbjct: 372 CEMEGYKIKKGWNINIDARSIHLDPTVYSEPHKFNPLRFEEEAKANSFLAFGMGGRTCLG 431

Query: 441 INMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 479
           + +AK  MLVFLHR    YRW V D DP +EK     RL+SG PI
Sbjct: 432 LALAKAMMLVFLHRFITTYRWEVVDEDPSIEKWTLFARLKSGYPI 470

[Close]

BLAST of CmaCh03G007260 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-58
Identity = 145/465 (31.18%), Postives = 241/465 (51.83%), Query Frame = 0


Query: 18  TTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETFSFLSANNSTRGCYQFVR 77
           T  AA L + +L  ++ V  R  S  +   PG +G P+VGETF   S + +    +   +
Sbjct: 9   TLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV---FFAAK 68

Query: 78  LRRHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGYVKSMGDAVGEKSLLCVSHE 137
            RR+G  F+T + G   V + S E AK +L      F   +  S    +G++++     +
Sbjct: 69  QRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGD 128

Query: 138 DHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGKSFVLLDFSMKITFDSMCNM 197
            H ++R+L+   F   ++   V   + +  + L     DG            TF+     
Sbjct: 129 YHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVALIS 188

Query: 198 LMSLRDESTLRQIEKDCTAVTD-AMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRR 257
           ++  +DE   R+  K C  + +    S P  +PGT ++K +KAR  L + L  I+S+RR+
Sbjct: 189 ILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQ 248

Query: 258 GEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENRE 317
              S  D L S ++  +      L D +I DN++ +I A++ TTA+ + W +K+L +N  
Sbjct: 249 NPSSHTDLLGSFMEDKA-----GLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPT 308

Query: 318 AQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRL 377
             E + EEQ++I + K++GE LT ED  KM    +V++ETLR + +L +  R A+ D   
Sbjct: 309 VLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEY 368

Query: 378 EGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINM 437
           EG+ I KGW V      IH++ D++ DP +F+PSRF+   KP +F+PFGSG  +C G  +
Sbjct: 369 EGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNEL 428

Query: 438 AKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPITLK 482
           AKL + V +H +T  YRW++  P   ++        ++G PI L+
Sbjct: 429 AKLEISVLIHHLTTKYRWSIVGPSDGIQ-YGPFALPQNGLPIALE 460

[Close]

BLAST of CmaCh03G007260 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 216.9 bits (551), Expect = 3.7e-56
Identity = 131/410 (31.95%), Postives = 216/410 (52.68%), Query Frame = 0


Query: 48  PGHLGLPFVGETFSFLSANNSTRGCYQFVRLRRHGRWFRTRLFGKIHVFVPSAEGAKAIL 107
           PG +G P+VGETF   S + +    +   + +R+G  F+T + G   V + S E AK +L
Sbjct: 38  PGTMGWPYVGETFQLYSQDPNV---FFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVL 97

Query: 108 ANDFVQFNKGYVKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLS 167
                 F   +  S    +G++++     + H ++R+L+   F   S+   V   + +  
Sbjct: 98  VTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQ 157

Query: 168 KRLQKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTD-AMLSFPF 227
             L+    +G            TF+     +   +DE   R+  K C  + +    S P 
Sbjct: 158 DSLRSW--EGTMINTYQEMKTYTFNVALLSIFG-KDEVLYREDLKRCYYILEKGYNSMPV 217

Query: 228 MIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIM 287
            +PGT ++K +KAR  L + L  I+S RR+   S  D L S +        ++L D +I 
Sbjct: 218 NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM-----GDKEELTDEQIA 277

Query: 288 DNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKM 347
           DN++ +I A++ TTA+ M W +K+L EN    E + EEQ++I + KE+GE LT  D  KM
Sbjct: 278 DNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKM 337

Query: 348 SFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQ 407
              S+V++ETLR++++L +  R A+ D   EG+ I KGW V      IH+  D++ +P +
Sbjct: 338 PLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGK 397

Query: 408 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTV 457
           F+PSRF+   KP +F+PFG+G  +C G  +AKL M + +H +T  Y W++
Sbjct: 398 FDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSI 436

[Close]

BLAST of CmaCh03G007260 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 216.5 bits (550), Expect = 4.8e-56
Identity = 130/441 (29.48%), Postives = 228/441 (51.70%), Query Frame = 0


Query: 47  IPGHLGLPFVGETFSFLSANNSTRGCYQFV--RLRRHGRWFRTRLFGKIHVFVPSAEGAK 106
           +PG +GLP++GET  F  A  S R    FV  R+ +HG  F+TR+ G   + V  AE  +
Sbjct: 46  LPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEANR 105

Query: 107 AILANDFVQFNKGYVKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDD 166
            IL+N+F      +  S    +G   ++    E HR +R ++A+  S   L   + +  D
Sbjct: 106 LILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCD 165

Query: 167 MLSKRLQKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSF 226
            +    +   +  +   L   +  +TF  +   L  ++ E  + ++ +    V + + + 
Sbjct: 166 TVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKVEIGMLEVFE---RVLEGVFAL 225

Query: 227 PFMIPGTRYYKGIKARNRLMERL-GEIISRRR----RGEESPEDFLQSMLQRDSYPPDQK 286
           P   P +++ +  KAR  +   L G++  +RR     G E P   L S L  +       
Sbjct: 226 PVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKEGAEKPNTTLFSRLVEELI--KGV 285

Query: 287 LDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLT 346
           + + E++DN++ L+ A+  TT+ AM  + K L ++   ++ L +E   I   K +GE LT
Sbjct: 286 ITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLT 345

Query: 347 LEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPD 406
           +ED+ KM +  +VV+ET+R+S  +    R A+ D    G+ I KGW +       HY+P+
Sbjct: 346 VEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPE 405

Query: 407 LYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDP 466
           +++DPM F+P+RFD+  + Y+++PFG GPR C G  +AK+++LVF+H +  G+ W++  P
Sbjct: 406 IFQDPMSFDPTRFDKPIQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYP 465

Query: 467 DPCLEKKAHIPRLRSGCPITL 481
           D  +     +P    G PI +
Sbjct: 466 DETISMDP-LPFPSLGMPIKI 480

[Close]

BLAST of CmaCh03G007260 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 216.1 bits (549), Expect = 6.3e-56
Identity = 139/437 (31.81%), Postives = 228/437 (52.17%), Query Frame = 0


Query: 18  TTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETFSFLSANNSTRGCYQFVR 77
           T  AA L + +L  ++ V  R  S  +   PG +G P+VGETF   S + +    +   +
Sbjct: 9   TLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV---FFAAK 68

Query: 78  LRRHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGYVKSMGDAVGEKSLLCVSHE 137
            RR+G  F+T + G   V + S E AK +L      F   +  S    +G++++     +
Sbjct: 69  QRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGD 128

Query: 138 DHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGKSFVLLDFSMKITFDSMCNM 197
            H ++R+L+   F   ++   V   + +  + L     DG            TF+     
Sbjct: 129 YHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVALIS 188

Query: 198 LMSLRDESTLRQIEKDCTAVTD-AMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRR 257
           ++  +DE   R+  K C  + +    S P  +PGT ++K +KAR  L + L  I+S+RR+
Sbjct: 189 ILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQ 248

Query: 258 GEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENRE 317
              S  D L S ++  +      L D +I DN++ +I A++ TTA+ + W +K+L +N  
Sbjct: 249 NPSSHTDLLGSFMEDKA-----GLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPT 308

Query: 318 AQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRL 377
             E + EEQ++I + K++GE LT ED  KM    +V++ETLR + +L +  R A+ D   
Sbjct: 309 VLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEY 368

Query: 378 EGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINM 437
           EG+ I KGW V      IH++ D++ DP +F+PSRF+   KP +F+PFGSG  +C G  +
Sbjct: 369 EGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNEL 428

Query: 438 AKLTMLVFLHRMTGGYR 454
           AKL + V +H +T  YR
Sbjct: 429 AKLEISVLIHHLTTKYR 433

The following BLAST results are available for this feature:

BLAST of CmaCh03G007260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FH76	1.5e-57	31.18	Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ...	[more]
Q50EK1	1.1e-55	31.25	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q949P1	5.2e-55	31.95	Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ...	[more]
A2Z212	2.0e-54	31.39	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]
Q0J185	2.0e-54	31.39	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70...	[more]

BLAST of CmaCh03G007260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IMR3	9.9e-283	99.59	abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1IQ66	4.3e-278	94.02	abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GG59	8.1e-277	98.35	abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1GG87	3.5e-272	92.80	abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A5A7SHU1	3.1e-252	90.10	Abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

BLAST of CmaCh03G007260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022978466.1	2.0e-282	99.59	abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima]	[more]
XP_023543351.1	1.8e-278	98.36	abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022978465.1	8.9e-278	94.02	abscisic acid 8'-hydroxylase 3-like isoform X1 [Cucurbita maxima]	[more]
XP_022950585.1	1.7e-276	98.35	abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita moschata] >KAG7034125....	[more]
KAG6603947.1	3.2e-275	98.14	Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmaCh03G007260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19630.1	1.1e-111	46.67	cytochrome P450, family 722, subfamily A, polypeptide 1	[more]
AT5G45340.1	1.0e-58	31.18	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	3.7e-56	31.95	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT2G42850.1	4.8e-56	29.48	cytochrome P450, family 718	[more]
AT5G45340.2	6.3e-56	31.81	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 314..334
None	No IPR available	PIRSR	PIRSR000209-1	PIRSR000209-1	coord: 45..437 e-value: 3.2E-28 score: 95.7
None	No IPR available	PANTHER	PTHR24286:SF235	CYTOCHROME P450 FAMILY PROTEIN	coord: 19..483
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 19..483
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 431..442 score: 32.3 coord: 422..431 score: 58.94 coord: 351..362 score: 36.96 coord: 295..312 score: 26.21
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 49..463 e-value: 3.9E-66 score: 223.7
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 284..301 score: 23.39 coord: 304..330 score: 20.66 coord: 431..454 score: 21.8 coord: 390..414 score: 30.6 coord: 421..431 score: 53.98
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 37..484 e-value: 6.2E-105 score: 353.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 37..481
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 424..433

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G007260.1	CmaCh03G007260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen