CmaCh03G005660 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G005660
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAcyl-protein thioesterase 1
LocationCma_Chr03: 5200275 .. 5203309 (-)
RNA-Seq ExpressionCmaCh03G005660
SyntenyCmaCh03G005660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAATTTTTATGAGGACGTGAATGAGAAGCCGTTCAAGAAAAATTCTCAATCTCCGCAGTCCCCACTTCCGCATTAACTCAAGCGGAGAAGACTCGATGGCAATGGGAGATCAGCTATGGCTACTGCATCTGTAAGTCAACAATTAACGAAGTCGATCGCACTCTCTGCAGCCACCTTCAGCGCAACCATTCTCTTGGTTCTATTTCATCGCACCCATCCATCTTCTTCGATAACCCCAGATCCAATGGCGGCTCGAAGCTTCATCCTCTGGCTTCATGGATTGGGAGATTCAGGTCCTGCCAACGAGCCAATCAAGAATCTCTTCACTTCATCTGAGTTCAAACGCACTTCCTGGTCTTTCCCCTCTGCTCCTTCCAACCCCGTCACGTGTAATTGTAAGTTCTCACGATTTAATTTTTAGGGTTTTTTTTCCTCTGTCTGTGAATAGCCGTGTTCTGGATGTTGAATTTGTGTGGGAAATTATGTTTTTCATACTACACAATCTCATGAAACACTATTAAACCTTTAAACTCTAAAATTTAGAAATGAAGATTCGATCAAAACAATTCAACCCGAAGCCATAAACTGACGGAATTCAACAGGAGAGAAAAAATTATGTGTATACCTGAAAGAGAGAACCCATGGATGGGTTGGTTCTGAATCGTGTAAAGAAGAAGACTTAGAGAGAATTTAGTTGGAGAACAAAGTGGTAACATTGTTTGATCGATGACATAAGCAAAAAGGTAAAGAACTTGGTTTGTAACGGATGGATTACATCTCATTTGTAACTTTGTGTGCTCGTATTATATCAAATTTAGGTGAAATTACTTGTGGCGGTTTAATTTAATAAATATATTTTTGGGTTATTGCATGAATTGTCCTCCCCTGTTGAGGGAGTTTAGTTTTGTGGGCTTGATTTGTTTGTATTAATTGACCCAATTTCTTGTTCGAATCAAGGGAATTATATGCTTCGTGTGTTGGATTTGTAATGATGAACTTGAATTGCCCTGTTGGTCTTGAACTGAGGAAGATAGAACTTGTGCTTTAATGCTCTAGAATGGTATCGGACGAAAGTGCGATGCCTGATAGATGATTTGTCAGTCCTTCTCTAGTGAATGGCTTGAACAGCTCAGGGTGATTTTAACCTGGAAACTAAAAGGGAAAGCCAAGAATATGACGGGGCTATGATTGCAACCCCTACTGCCACTTGTTGTCTTCAGCATCTTCTCTTTTAAAAAAGTGTTTTAATCTTCTTATTCTTCAATCAGCTTATTTCTTTTCCACTTGTAGATGGTGCGGTGATGCCTTCATGGTTTGACATTCATGAGATTCCAGTAACCGCTGTAAGTTAAGGCTTTAAGCCATCTTTATATACTCTTTCCTTCCTGAAACGGTCGTCCCATGTGAAGAATTTTCCTTTTGCTGCAAAGATAAATTATTATTTTTGAACTCTTCAATTCTTAAAAAGGTGCTAATACCTCTAAGAAGACGCATCTTTGCTTAAAATTTCTGGATATTAACTCTAAATTCTATCGACAATGGACTATTTGTTGGGTGTTTTATAGCATTTTTCTTTAAAATTTTCCATTCTTTCATGAAATAGGATTCTCCAAAGGCTGAAAGGTCGGTGCTTGAAGCAGTACAGAGTGTGCATGCAAAGATAGACAAGGTGGTAGATGGTGGCATAAGTCCAAATAACATATTTGTGTGTGGATTCAGTCAAGGAGGTTTTAGTCTCTCTCTCCTCCCATCTATACTTGCTTTAGATTTGTATATAAAACTCTTGTATGATAGTTTCTTCTTATTGCTGTTAACTCCTTGCAGGTGCCTTGACATTGGCTAGTGTTCTGCTATATCCAAAGACTCTAGGAGGAGGTGCGGTATTTAGTGGATGGGTTCCTTTCAATTCAACAATTATTGACCGTGTACATCCCGATGCAAAAAGGGTACTCACTCTTTCATTAACAGATCCTTAGTTACAGCTTTGTTGAAAAGTTGGAATTATAATGTGCATTCATGGTGGATGGAGTGGCTGTCATTTTAGATACTTTTGCTGTAAAAAGGGTGAAGGTGTGAGATCACACGTCGGTTGGAGAGGGGAACAAAGCATTTTTGTGTGGAAACCTCTCCCTCACAAACGTATTTTAAAACTTTGAGGGGAAGCCCAGAAGAGAAAGTCCAAAACGACAATATCTGCTAGCGGTAAATTTAGACTATTACAAATGGTATCAGAGCCAGTCACTAGGCAGTGTGCCAGCGTCACTGCTAGCGAGGAGGCTGGGTCTCCAAAGAGGGTGGATTGTGAGATCTCACATCGGTTAGAGAAGGGACGAAGCATTCCTTATAACGGTGTGGAAACCTCTTCCTAACAAACGCATTTTAAAATCTTGAGGGAAAGTCGCTTAAATTTGACAATATATATTAGTGGTGGGCTTAGATTGTTACGGAGACACAATTTCTAAGATTTGAATGTTTGTACAGACACCAATTTTATGGTCTCATGGAATGGACGATAGAACCGTATTGTTTGAAGCTGGACAAGCCGGGCCACCGTTCCTGGAAAAGGCTGGACTAAGCTGCGAATTTAAGGTAATTTGCTTGACTTCCTCTTCTACAAATGTGTATGAACTTTTGAGATTGAAAGTCTAAAATGGTTCGGTCATCTTATTTTGAAGGCGTATCCCGGTCTTGGTCATTCGATAAGCACGGAGGAGCTCAAACACCTTGAATCGTGGATCAAGAGCCGCCTGCAAAGCTCTTCCTGAACCCTTTGAACCCTTTTGCTGGATGACATTCATCATCTTCACATCATTATGTTATTAACAACTTTGACACGCAGTCTAAAAGAAATTTTGTTGGAAAAGCGTGAAGCTGGGTTATAATGTCCAATATTTTGAAGCATTGTAGTTGACTCATAAGGAAGCAAAGAATGATGATTATTCCACATGAGCTAATAATATAATATGCTTTGTTCATCAATTACGTGTTTGGATCTTTAATACGTGGATTACACAAATCATACGTGTCAATGGC

mRNA sequence

TAAAATTTTTATGAGGACGTGAATGAGAAGCCGTTCAAGAAAAATTCTCAATCTCCGCAGTCCCCACTTCCGCATTAACTCAAGCGGAGAAGACTCGATGGCAATGGGAGATCAGCTATGGCTACTGCATCTGTAAGTCAACAATTAACGAAGTCGATCGCACTCTCTGCAGCCACCTTCAGCGCAACCATTCTCTTGGTTCTATTTCATCGCACCCATCCATCTTCTTCGATAACCCCAGATCCAATGGCGGCTCGAAGCTTCATCCTCTGGCTTCATGGATTGGGAGATTCAGGTCCTGCCAACGAGCCAATCAAGAATCTCTTCACTTCATCTGAGTTCAAACGCACTTCCTGGTCTTTCCCCTCTGCTCCTTCCAACCCCGTCACGTGTAATTATGGTGCGGTGATGCCTTCATGGTTTGACATTCATGAGATTCCAGTAACCGCTGATTCTCCAAAGGCTGAAAGGTCGGTGCTTGAAGCAGTACAGAGTGTGCATGCAAAGATAGACAAGGTGGTAGATGGTGGCATAAGTCCAAATAACATATTTGTGTGTGGATTCAGTCAAGGAGGTGCCTTGACATTGGCTAGTGTTCTGCTATATCCAAAGACTCTAGGAGGAGGTGCGGTATTTAGTGGATGGGTTCCTTTCAATTCAACAATTATTGACCGTGTACATCCCGATGCAAAAAGGACACCAATTTTATGGTCTCATGGAATGGACGATAGAACCGTATTGTTTGAAGCTGGACAAGCCGGGCCACCGTTCCTGGAAAAGGCTGGACTAAGCTGCGAATTTAAGGCGTATCCCGGTCTTGGTCATTCGATAAGCACGGAGGAGCTCAAACACCTTGAATCGTGGATCAAGAGCCGCCTGCAAAGCTCTTCCTGAACCCTTTGAACCCTTTTGCTGGATGACATTCATCATCTTCACATCATTATGTTATTAACAACTTTGACACGCAGTCTAAAAGAAATTTTGTTGGAAAAGCGTGAAGCTGGGTTATAATGTCCAATATTTTGAAGCATTGTAGTTGACTCATAAGGAAGCAAAGAATGATGATTATTCCACATGAGCTAATAATATAATATGCTTTGTTCATCAATTACGTGTTTGGATCTTTAATACGTGGATTACACAAATCATACGTGTCAATGGC

Coding sequence (CDS)

ATGGCTACTGCATCTGTAAGTCAACAATTAACGAAGTCGATCGCACTCTCTGCAGCCACCTTCAGCGCAACCATTCTCTTGGTTCTATTTCATCGCACCCATCCATCTTCTTCGATAACCCCAGATCCAATGGCGGCTCGAAGCTTCATCCTCTGGCTTCATGGATTGGGAGATTCAGGTCCTGCCAACGAGCCAATCAAGAATCTCTTCACTTCATCTGAGTTCAAACGCACTTCCTGGTCTTTCCCCTCTGCTCCTTCCAACCCCGTCACGTGTAATTATGGTGCGGTGATGCCTTCATGGTTTGACATTCATGAGATTCCAGTAACCGCTGATTCTCCAAAGGCTGAAAGGTCGGTGCTTGAAGCAGTACAGAGTGTGCATGCAAAGATAGACAAGGTGGTAGATGGTGGCATAAGTCCAAATAACATATTTGTGTGTGGATTCAGTCAAGGAGGTGCCTTGACATTGGCTAGTGTTCTGCTATATCCAAAGACTCTAGGAGGAGGTGCGGTATTTAGTGGATGGGTTCCTTTCAATTCAACAATTATTGACCGTGTACATCCCGATGCAAAAAGGACACCAATTTTATGGTCTCATGGAATGGACGATAGAACCGTATTGTTTGAAGCTGGACAAGCCGGGCCACCGTTCCTGGAAAAGGCTGGACTAAGCTGCGAATTTAAGGCGTATCCCGGTCTTGGTCATTCGATAAGCACGGAGGAGCTCAAACACCTTGAATCGTGGATCAAGAGCCGCCTGCAAAGCTCTTCCTGA

Protein sequence

MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSGPANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSVLEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFNSTIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSISTEELKHLESWIKSRLQSSS
Homology
BLAST of CmaCh03G005660 vs. ExPASy Swiss-Prot
Match: Q8GYK2 (Probable carboxylesterase SOBER1-like OS=Arabidopsis thaliana OX=3702 GN=At4g22300 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 1.9e-96
Identity = 165/246 (67.07%), Postives = 202/246 (82.11%), Query Frame = 0

Query: 13  SIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSGPANEPIKNLFTS 72
           +IAL ++ F      +       +S++      AR+FILWLHGLGDSGPANEPIK LF S
Sbjct: 14  TIALLSSVFFFAFFFLNKSDVSSTSALRNRDSMARTFILWLHGLGDSGPANEPIKTLFRS 73

Query: 73  SEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSVLEAVQSVHAKID 132
            EF+ T W FPSAP NPV+CNYGAVMPSWFDI E+P+TA SPK E S+L+AV++VHA ID
Sbjct: 74  QEFRNTKWLFPSAPPNPVSCNYGAVMPSWFDIPELPLTAGSPKDESSLLKAVKNVHAIID 133

Query: 133 KVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFNSTIIDRVHPDAK 192
           K + GGI+P N+++CGFSQGGALTLASVLLYPKT+GGGAVFSGW+PFNS+I ++   DAK
Sbjct: 134 KEIAGGINPENVYICGFSQGGALTLASVLLYPKTIGGGAVFSGWIPFNSSITNQFTEDAK 193

Query: 193 RTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSISTEELKHLESWIKS 252
           +TPILWSHG+DD+TVLFEAGQA  PFL++AG++CEFKAYPGLGHSIS EEL++LESW+K 
Sbjct: 194 KTPILWSHGIDDKTVLFEAGQAALPFLQQAGVTCEFKAYPGLGHSISNEELQYLESWLKQ 253

Query: 253 RLQSSS 259
           R+QSSS
Sbjct: 254 RMQSSS 259

BLAST of CmaCh03G005660 vs. ExPASy Swiss-Prot
Match: Q0J969 (Probable carboxylesterase Os04g0669500 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0669500 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.2e-96
Identity = 168/261 (64.37%), Postives = 208/261 (79.69%), Query Frame = 0

Query: 6   VSQQLTKSIALSAATFSATILLVLFHRTHPSSS----ITPDPMAA-----RSFILWLHGL 65
           +S +L    +L+AA  +A++L     R  P S     + P PMAA     RSF+LWLHGL
Sbjct: 1   MSSRLRFLFSLAAAIAAASLLAAALRRRAPPSGLAARLVPAPMAAAAARNRSFVLWLHGL 60

Query: 66  GDSGPANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKA 125
           GDSGPANEPI+N F++ EF+ T W+FPSAP++PV+CN+GAVMPSWFDIHE+P+++ SP+ 
Sbjct: 61  GDSGPANEPIRNFFSAPEFRLTKWAFPSAPNSPVSCNHGAVMPSWFDIHELPMSSGSPQD 120

Query: 126 ERSVLEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGW 185
           +  VL+AV++VHA IDK V  GI P NIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGW
Sbjct: 121 DSGVLKAVENVHAMIDKEVADGIPPENIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGW 180

Query: 186 VPFNSTIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGH 245
           +PF S++ +R+ P+A++TPILWSHG+ D  VLFEAGQAGPPFL+ AG SCEFKAYPGLGH
Sbjct: 181 LPFGSSVTERISPEARKTPILWSHGIADNVVLFEAGQAGPPFLQNAGFSCEFKAYPGLGH 240

Query: 246 SISTEELKHLESWIKSRLQSS 258
           SIS EEL  LESWIK+ L++S
Sbjct: 241 SISKEELYSLESWIKNHLKAS 261

BLAST of CmaCh03G005660 vs. ExPASy Swiss-Prot
Match: Q84WK4 (Carboxylesterase SOBER1 OS=Arabidopsis thaliana OX=3702 GN=SOBER1 PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.0e-86
Identity = 151/213 (70.89%), Postives = 174/213 (81.69%), Query Frame = 0

Query: 46  ARSFILWLHGLGDSGPANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIH 105
           AR+FILWLHGLGDSGPANEPI+  F SSE    SW FPSAP NPVTCN GAVM SWFD+ 
Sbjct: 2   ARTFILWLHGLGDSGPANEPIQTQFKSSELSNASWLFPSAPFNPVTCNNGAVMRSWFDVP 61

Query: 106 EIPVTADSPKAERSVLEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPK 165
           E+P    SP  E SVLEAV++VHA ID+ +  G +P N+F+CG SQGGALTLASVLLYPK
Sbjct: 62  ELPFKVGSPIDESSVLEAVKNVHAIIDQEIAEGTNPENVFICGLSQGGALTLASVLLYPK 121

Query: 166 TLGGGAVFSGWVPFNSTIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLS 225
           TLGGGAV SGWVPF S+II +   +AK+TPILWSHG DDR VLFEAGQA  PFL++AG++
Sbjct: 122 TLGGGAVLSGWVPFTSSIISQFPEEAKKTPILWSHGTDDRMVLFEAGQAALPFLKEAGVT 181

Query: 226 CEFKAYPGLGHSISTEELKHLESWIKSRLQSSS 259
           CEFKAYPGLGHSIS +ELK++ESWIK RL+ SS
Sbjct: 182 CEFKAYPGLGHSISNKELKYIESWIKRRLKGSS 214

BLAST of CmaCh03G005660 vs. ExPASy Swiss-Prot
Match: Q7XR62 (Probable inactive carboxylesterase Os04g0669700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0669700 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.2e-60
Identity = 110/210 (52.38%), Postives = 146/210 (69.52%), Query Frame = 0

Query: 49  FILWLHGLGDSGPANEPIKNLF-TSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEI 108
           F++WLHGLGD+G ANE + + F T++ F    W+FP+AP+ PVTCN G +MPSWFDIH+ 
Sbjct: 13  FVVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRGMLMPSWFDIHDA 72

Query: 109 PVTADSPKAERSVLEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTL 168
           P+T+ S + E  VL AVQSVHA ID+ +  G +P ++FV G SQGGAL +ASVLL+PKTL
Sbjct: 73  PITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVFVFGLSQGGALGIASVLLHPKTL 132

Query: 169 GGGAVFSGWVPFNSTIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCE 228
           GG AVFSG++PFNS+   RV   AK+TP+LW HG     +  + G+ G  FL   G+SCE
Sbjct: 133 GGCAVFSGFLPFNSSFAVRVTAQAKKTPVLWIHGQAGSLIPIKEGRDGIKFLRGLGMSCE 192

Query: 229 FKAYPGLGHSISTEELKHLESWIKSRLQSS 258
           FK Y  LGHS+   EL + + W++  L  S
Sbjct: 193 FKVYDRLGHSLEYYELDYCQRWVEKILHRS 222

BLAST of CmaCh03G005660 vs. ExPASy Swiss-Prot
Match: Q0J968 (Probable carboxylesterase Os04g0669600 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0669600 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 3.9e-57
Identity = 103/210 (49.05%), Postives = 141/210 (67.14%), Query Frame = 0

Query: 50  ILWLHGLGDSG-PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIP 109
           +LWLHG G +G  +   +   F + E     +SFP+AP++ + C  G V+ +WF I E+P
Sbjct: 12  VLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEVITAWFAIPEVP 71

Query: 110 VTADSPKAERSVLEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLG 169
           +TA + + E+ VL+AV+ VH  +D  V  G SP+NIFVCG SQGGAL +ASVLLYP TLG
Sbjct: 72  ITARTARDEKEVLKAVERVHEMLDGEVAAGTSPSNIFVCGLSQGGALAIASVLLYPMTLG 131

Query: 170 GGAVFSGWVPFNSTIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEF 229
           G  VFSG +P + T  + +  +A++TP+LW HGM D  VLFEAG AG  FL++ G+ CEF
Sbjct: 132 GCVVFSGSLPLSKTFAESIPSEARKTPVLWFHGMADGVVLFEAGHAGCAFLQEIGMHCEF 191

Query: 230 KAYPGLGHSISTEELKHLESWIKSRLQSSS 259
           KAYP LGH++  EEL++   WIK RL   +
Sbjct: 192 KAYPALGHTLVDEELQYFRQWIKDRLSQGT 221

BLAST of CmaCh03G005660 vs. ExPASy TrEMBL
Match: A0A6J1IJ70 (carboxylesterase SOBER1-like OS=Cucurbita maxima OX=3661 GN=LOC111477921 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 3.9e-145
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG
Sbjct: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV
Sbjct: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST
Sbjct: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. ExPASy TrEMBL
Match: A0A6J1GGK2 (probable carboxylesterase Os04g0669500 OS=Cucurbita moschata OX=3662 GN=LOC111453707 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.4e-142
Identity = 252/258 (97.67%), Postives = 255/258 (98.84%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           MATASV QQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG
Sbjct: 1   MATASVRQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIKNLFTS EFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAE SV
Sbjct: 61  PANEPIKNLFTSPEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKV+DGGISP+NIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVIDGGISPSNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STI+DRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST
Sbjct: 181 STILDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. ExPASy TrEMBL
Match: A0A0A0KH84 (Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G511810 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.7e-132
Identity = 235/258 (91.09%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           M T SV QQLT SI LS AT +ATIL VLFH  H SSS TP PMAAR+FILWLHGLGDSG
Sbjct: 1   MPTPSVRQQLTNSITLSVATLAATILFVLFHSPHSSSSTTPHPMAARTFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIK+LFTS EFKRTSWSFPSAP+NPVTCNYGAVMPSWFDIHEIPVTADSPKAE SV
Sbjct: 61  PANEPIKSLFTSPEFKRTSWSFPSAPNNPVTCNYGAVMPSWFDIHEIPVTADSPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKVVDGGI+PNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVVDGGITPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STIIDR+HPDAKRTPILWSHG+DDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIS 
Sbjct: 181 STIIDRIHPDAKRTPILWSHGIDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSISM 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. ExPASy TrEMBL
Match: A0A1S3B091 (LOW QUALITY PROTEIN: probable carboxylesterase SOBER1-like OS=Cucumis melo OX=3656 GN=LOC103484650 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.2e-132
Identity = 236/258 (91.47%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           M T SV  QLTK I LS AT +ATIL VLFH  H SSS TP PMAARSFILWLHGLGDSG
Sbjct: 1   MPTPSVRLQLTKPITLSVATLAATILFVLFHSPHSSSSTTPHPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIK+LFTS EFKRTSWSFPSAP+NPVTCNYGAVMPSWFDIHEIPVTADSPKAE SV
Sbjct: 61  PANEPIKSLFTSPEFKRTSWSFPSAPNNPVTCNYGAVMPSWFDIHEIPVTADSPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STIIDR+HPDAKRTPILWSHG+DDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIS 
Sbjct: 181 STIIDRIHPDAKRTPILWSHGIDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSISM 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. ExPASy TrEMBL
Match: A0A5D3CRW4 (Putative carboxylesterase SOBER1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006080 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 2.9e-132
Identity = 235/258 (91.09%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           M T SV  QLTK I LS AT +ATIL VLFH  H SSS TP PMAARSFILWLHGLGDSG
Sbjct: 1   MPTPSVRLQLTKPITLSVATLAATILFVLFHSPHSSSSTTPHPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIK+LFTS EFKRTSWSFPSAP+NPVTCNYGAVMPSWFDIHEIPVTADSPKAE SV
Sbjct: 61  PANEPIKSLFTSPEFKRTSWSFPSAPNNPVTCNYGAVMPSWFDIHEIPVTADSPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STIIDR+HPDAKRTPILWSHG+DDRT+LFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIS 
Sbjct: 181 STIIDRIHPDAKRTPILWSHGIDDRTILFEAGQAGPPFLEKAGLSCEFKAYPGLGHSISM 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. NCBI nr
Match: XP_022977687.1 (carboxylesterase SOBER1-like [Cucurbita maxima])

HSP 1 Score: 523.9 bits (1348), Expect = 8.1e-145
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG
Sbjct: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV
Sbjct: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST
Sbjct: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. NCBI nr
Match: XP_022950684.1 (probable carboxylesterase Os04g0669500 [Cucurbita moschata] >KAG6603810.1 putative carboxylesterase SOBER1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514.6 bits (1324), Expect = 4.9e-142
Identity = 252/258 (97.67%), Postives = 255/258 (98.84%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           MATASV QQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG
Sbjct: 1   MATASVRQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIKNLFTS EFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAE SV
Sbjct: 61  PANEPIKNLFTSPEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKV+DGGISP+NIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVIDGGISPSNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STI+DRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST
Sbjct: 181 STILDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. NCBI nr
Match: KAG7033991.1 (putative carboxylesterase SOBER1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 511.1 bits (1315), Expect = 5.4e-141
Identity = 251/258 (97.29%), Postives = 254/258 (98.45%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           MATASV QQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG
Sbjct: 1   MATASVRQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIKNLFTS EFKRTSWSFPSAPSNPVTCNYGAVMPSWFDI EIPVTADSPKAE SV
Sbjct: 61  PANEPIKNLFTSLEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIREIPVTADSPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKV+DGGISP+NIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVIDGGISPSNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STI+DRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST
Sbjct: 181 STILDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. NCBI nr
Match: XP_023545113.1 (probable carboxylesterase SOBER1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 510.8 bits (1314), Expect = 7.1e-141
Identity = 252/258 (97.67%), Postives = 253/258 (98.06%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           MATASV QQLTKSIALSAATFSATILLVLFHRTHPSSSIT DPMAARSFILWLHGLGDSG
Sbjct: 1   MATASVRQQLTKSIALSAATFSATILLVLFHRTHPSSSITLDPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIKNLFTS  FKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAE SV
Sbjct: 61  PANEPIKNLFTSPAFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STI+DRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST
Sbjct: 181 STILDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIKSRLQSSS
Sbjct: 241 EELKHLESWIKSRLQSSS 258

BLAST of CmaCh03G005660 vs. NCBI nr
Match: XP_038880927.1 (carboxylesterase SOBER1-like [Benincasa hispida])

HSP 1 Score: 486.9 bits (1252), Expect = 1.1e-133
Identity = 238/258 (92.25%), Postives = 244/258 (94.57%), Query Frame = 0

Query: 1   MATASVSQQLTKSIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSG 60
           MAT SV QQLTKSIALS AT + TILLVLFH  HPSSS  PDPMAARSFILWLHGLGDSG
Sbjct: 1   MATVSVRQQLTKSIALSVATLALTILLVLFHSPHPSSSTIPDPMAARSFILWLHGLGDSG 60

Query: 61  PANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSV 120
           PANEPIKNLFTS EFKRTSWSFPSAP NPVTCNYGAVMPSWFDIHEIPV+AD+PKAE SV
Sbjct: 61  PANEPIKNLFTSPEFKRTSWSFPSAPKNPVTCNYGAVMPSWFDIHEIPVSADTPKAESSV 120

Query: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180
           LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN
Sbjct: 121 LEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFN 180

Query: 181 STIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSIST 240
           STIIDR+HPDAKRTPILWSHG+DDRTVLFEAGQAG PFLEKAGLSCEFKAYPGLGHSIS 
Sbjct: 181 STIIDRIHPDAKRTPILWSHGIDDRTVLFEAGQAGLPFLEKAGLSCEFKAYPGLGHSISM 240

Query: 241 EELKHLESWIKSRLQSSS 259
           EELKHLESWIK+RLQSSS
Sbjct: 241 EELKHLESWIKNRLQSSS 258

BLAST of CmaCh03G005660 vs. TAIR 10
Match: AT4G22300.1 (carboxylesterases )

HSP 1 Score: 353.6 bits (906), Expect = 1.3e-97
Identity = 165/246 (67.07%), Postives = 202/246 (82.11%), Query Frame = 0

Query: 13  SIALSAATFSATILLVLFHRTHPSSSITPDPMAARSFILWLHGLGDSGPANEPIKNLFTS 72
           +IAL ++ F      +       +S++      AR+FILWLHGLGDSGPANEPIK LF S
Sbjct: 14  TIALLSSVFFFAFFFLNKSDVSSTSALRNRDSMARTFILWLHGLGDSGPANEPIKTLFRS 73

Query: 73  SEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIHEIPVTADSPKAERSVLEAVQSVHAKID 132
            EF+ T W FPSAP NPV+CNYGAVMPSWFDI E+P+TA SPK E S+L+AV++VHA ID
Sbjct: 74  QEFRNTKWLFPSAPPNPVSCNYGAVMPSWFDIPELPLTAGSPKDESSLLKAVKNVHAIID 133

Query: 133 KVVDGGISPNNIFVCGFSQGGALTLASVLLYPKTLGGGAVFSGWVPFNSTIIDRVHPDAK 192
           K + GGI+P N+++CGFSQGGALTLASVLLYPKT+GGGAVFSGW+PFNS+I ++   DAK
Sbjct: 134 KEIAGGINPENVYICGFSQGGALTLASVLLYPKTIGGGAVFSGWIPFNSSITNQFTEDAK 193

Query: 193 RTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFKAYPGLGHSISTEELKHLESWIKS 252
           +TPILWSHG+DD+TVLFEAGQA  PFL++AG++CEFKAYPGLGHSIS EEL++LESW+K 
Sbjct: 194 KTPILWSHGIDDKTVLFEAGQAALPFLQQAGVTCEFKAYPGLGHSISNEELQYLESWLKQ 253

Query: 253 RLQSSS 259
           R+QSSS
Sbjct: 254 RMQSSS 259

BLAST of CmaCh03G005660 vs. TAIR 10
Match: AT4G22305.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 7.4e-88
Identity = 151/213 (70.89%), Postives = 174/213 (81.69%), Query Frame = 0

Query: 46  ARSFILWLHGLGDSGPANEPIKNLFTSSEFKRTSWSFPSAPSNPVTCNYGAVMPSWFDIH 105
           AR+FILWLHGLGDSGPANEPI+  F SSE    SW FPSAP NPVTCN GAVM SWFD+ 
Sbjct: 2   ARTFILWLHGLGDSGPANEPIQTQFKSSELSNASWLFPSAPFNPVTCNNGAVMRSWFDVP 61

Query: 106 EIPVTADSPKAERSVLEAVQSVHAKIDKVVDGGISPNNIFVCGFSQGGALTLASVLLYPK 165
           E+P    SP  E SVLEAV++VHA ID+ +  G +P N+F+CG SQGGALTLASVLLYPK
Sbjct: 62  ELPFKVGSPIDESSVLEAVKNVHAIIDQEIAEGTNPENVFICGLSQGGALTLASVLLYPK 121

Query: 166 TLGGGAVFSGWVPFNSTIIDRVHPDAKRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLS 225
           TLGGGAV SGWVPF S+II +   +AK+TPILWSHG DDR VLFEAGQA  PFL++AG++
Sbjct: 122 TLGGGAVLSGWVPFTSSIISQFPEEAKKTPILWSHGTDDRMVLFEAGQAALPFLKEAGVT 181

Query: 226 CEFKAYPGLGHSISTEELKHLESWIKSRLQSSS 259
           CEFKAYPGLGHSIS +ELK++ESWIK RL+ SS
Sbjct: 182 CEFKAYPGLGHSISNKELKYIESWIKRRLKGSS 214

BLAST of CmaCh03G005660 vs. TAIR 10
Match: AT1G18773.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: carboxylesterases (TAIR:AT4G22300.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 62.4 bits (150), Expect = 6.2e-10
Identity = 25/38 (65.79%), Postives = 33/38 (86.84%), Query Frame = 0

Query: 192 KRTPILWSHGMDDRTVLFEAGQAGPPFLEKAGLSCEFK 230
           + TP+LWSHG+D++ VLFEAGQA  PFL++AGL+ EFK
Sbjct: 23  EHTPVLWSHGIDEKAVLFEAGQAALPFLQQAGLTYEFK 60

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GYK21.9e-9667.07Probable carboxylesterase SOBER1-like OS=Arabidopsis thaliana OX=3702 GN=At4g223... [more]
Q0J9693.2e-9664.37Probable carboxylesterase Os04g0669500 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q84WK41.0e-8670.89Carboxylesterase SOBER1 OS=Arabidopsis thaliana OX=3702 GN=SOBER1 PE=1 SV=1[more]
Q7XR622.2e-6052.38Probable inactive carboxylesterase Os04g0669700 OS=Oryza sativa subsp. japonica ... [more]
Q0J9683.9e-5749.05Probable carboxylesterase Os04g0669600 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IJ703.9e-145100.00carboxylesterase SOBER1-like OS=Cucurbita maxima OX=3661 GN=LOC111477921 PE=4 SV... [more]
A0A6J1GGK22.4e-14297.67probable carboxylesterase Os04g0669500 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A0A0KH841.7e-13291.09Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G5118... [more]
A0A1S3B0912.2e-13291.47LOW QUALITY PROTEIN: probable carboxylesterase SOBER1-like OS=Cucumis melo OX=36... [more]
A0A5D3CRW42.9e-13291.09Putative carboxylesterase SOBER1-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
XP_022977687.18.1e-145100.00carboxylesterase SOBER1-like [Cucurbita maxima][more]
XP_022950684.14.9e-14297.67probable carboxylesterase Os04g0669500 [Cucurbita moschata] >KAG6603810.1 putati... [more]
KAG7033991.15.4e-14197.29putative carboxylesterase SOBER1-like protein [Cucurbita argyrosperma subsp. arg... [more]
XP_023545113.17.1e-14197.67probable carboxylesterase SOBER1-like [Cucurbita pepo subsp. pepo][more]
XP_038880927.11.1e-13392.25carboxylesterase SOBER1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G22300.11.3e-9767.07carboxylesterases [more]
AT4G22305.17.4e-8870.89alpha/beta-Hydrolases superfamily protein [more]
AT1G18773.16.2e-1065.79FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003140Phospholipase/carboxylesterase/thioesterasePFAMPF02230Abhydrolase_2coord: 42..251
e-value: 1.9E-45
score: 155.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 31..257
e-value: 2.3E-60
score: 206.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 43..255
NoneNo IPR availablePANTHERPTHR10655:SF62CARBOXYLESTERASE SOBER1coord: 50..258
NoneNo IPR availablePANTHERPTHR10655LYSOPHOSPHOLIPASE-RELATEDcoord: 50..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G005660.1CmaCh03G005660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009820 alkaloid metabolic process
biological_process GO:0002084 protein depalmitoylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0052689 carboxylic ester hydrolase activity
molecular_function GO:0008474 palmitoyl-(protein) hydrolase activity
molecular_function GO:0016787 hydrolase activity