CmaCh03G004680 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G004680
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	DNA topoisomerase 6 subunit A
Location	Cma_Chr03: 4733481 .. 4734743 (+)
RNA-Seq Expression	CmaCh03G004680
Synteny	CmaCh03G004680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGATAAGAGAAAGCGCCGGCGATCTGAACTCAATCCAGACGTTGAAGCTCAACTTCCCTTCAAGAACAGCCTCAAATCTGATTCTATCATTCTTCAGATTCTCAAAGAGTTCAGTGACTGCTCCACCGCCCCATCTTCCTCTTCATCTAAAACCCTCGACGACCTAGGCCTCTCCTCCTCCTGCCGCGAGGTCACCGATCTTTCGCTTTCTTCCGTGCAGTCCACAATTGAAGATCTTATCCTCAAAATTGCTCGTTCCATTCTTTCCGGCAATGGTTTTGCATTTGATGTTCCTTCTCGATCGGCGGCCAATCAATTGTACGTTCCGGAGCTCGATCGCATCGTTTTGAAAGACAAAACCGCCATTCGACCCTACGCCAATATTTCCACTGTTCGGAAGTCCACCATTACTACTCGCATTCTTCAGCTTGTCCACCAACTTTGCATAAAAAACATCCATGTGACCAAGCGAGATCTGTTCTACACGGATGTAAAGCTCTTCCAGGATCAGACTCAGTCGGATTCTGTGCTTGATGATGTTTCTTGCATTCTCGGCTGTACTCGGTCGAGTCTCAATGTGATTGCTGCGGAGAAAGGAGTGGTTGTGGGAAGGCTCATTTTCAGTGACAATGGGGATATGATCGATTGCACTAAAATGGGCATGGGCGGAAAAGCGATTCCGCCAAATATTGATAGAGTTGGAGATATGCAGAGTGATGCATTGTTTATCCTCTTAGTTGAGAAAGATGCCGCGTATATTAGGTTAGCTGAGGATAGGTTTTACAATCGCTTTCCATGTATTATTGTGACCGCTAAGGGACAACCAGATGTGGCCACGAGATTGTTCTTGAGGAAGATGAAAATGGAGCTGAAGCTCCCAGTGCTTGCGCTTGTAGACAGTGATCCATATGGGTTGAAGATTCTATCTGTCTATGGATGTGGATCAAAGAATATGTCTTATGACAGTGCTAATTTGACTACTCCTGATATCAAGTGGCTAGGAATAAGGCCGAGTGATTTGGATAAATATAATATACCGGAGCAGTGCAGGTTACCTATGACAGAGCAGGATATTAAGACTGGGAAAGATCTGTTAGAGGAAGATTTTGTTAAAAAGAATCCAGGTTGGGTTGAAGAGCTCACTTTAATGGTGAAGACGAAGCAGAAGGCTGAGATTCAGGCATTGAGCTCATTCGGGTTTCAGTATCTGTCGGAGGTTTATCTGCCATTGAAGCTGCAGCAGAAGGATTGGCTGTGA

mRNA sequence

ATGGCCGATAAGAGAAAGCGCCGGCGATCTGAACTCAATCCAGACGTTGAAGCTCAACTTCCCTTCAAGAACAGCCTCAAATCTGATTCTATCATTCTTCAGATTCTCAAAGAGTTCAGTGACTGCTCCACCGCCCCATCTTCCTCTTCATCTAAAACCCTCGACGACCTAGGCCTCTCCTCCTCCTGCCGCGAGGTCACCGATCTTTCGCTTTCTTCCGTGCAGTCCACAATTGAAGATCTTATCCTCAAAATTGCTCGTTCCATTCTTTCCGGCAATGGTTTTGCATTTGATGTTCCTTCTCGATCGGCGGCCAATCAATTGTACGTTCCGGAGCTCGATCGCATCGTTTTGAAAGACAAAACCGCCATTCGACCCTACGCCAATATTTCCACTGTTCGGAAGTCCACCATTACTACTCGCATTCTTCAGCTTGTCCACCAACTTTGCATAAAAAACATCCATGTGACCAAGCGAGATCTGTTCTACACGGATGTAAAGCTCTTCCAGGATCAGACTCAGTCGGATTCTGTGCTTGATGATGTTTCTTGCATTCTCGGCTGTACTCGGTCGAGTCTCAATGTGATTGCTGCGGAGAAAGGAGTGGTTGTGGGAAGGCTCATTTTCAGTGACAATGGGGATATGATCGATTGCACTAAAATGGGCATGGGCGGAAAAGCGATTCCGCCAAATATTGATAGAGTTGGAGATATGCAGAGTGATGCATTGTTTATCCTCTTAGTTGAGAAAGATGCCGCGTATATTAGGTTAGCTGAGGATAGGTTTTACAATCGCTTTCCATGTATTATTGTGACCGCTAAGGGACAACCAGATGTGGCCACGAGATTGTTCTTGAGGAAGATGAAAATGGAGCTGAAGCTCCCAGTGCTTGCGCTTGTAGACAGTGATCCATATGGGTTGAAGATTCTATCTGTCTATGGATGTGGATCAAAGAATATGTCTTATGACAGTGCTAATTTGACTACTCCTGATATCAAGTGGCTAGGAATAAGGCCGAGTGATTTGGATAAATATAATATACCGGAGCAGTGCAGGTTACCTATGACAGAGCAGGATATTAAGACTGGGAAAGATCTGTTAGAGGAAGATTTTGTTAAAAAGAATCCAGGTTGGGTTGAAGAGCTCACTTTAATGGTGAAGACGAAGCAGAAGGCTGAGATTCAGGCATTGAGCTCATTCGGGTTTCAGTATCTGTCGGAGGTTTATCTGCCATTGAAGCTGCAGCAGAAGGATTGGCTGTGA

Coding sequence (CDS)

ATGGCCGATAAGAGAAAGCGCCGGCGATCTGAACTCAATCCAGACGTTGAAGCTCAACTTCCCTTCAAGAACAGCCTCAAATCTGATTCTATCATTCTTCAGATTCTCAAAGAGTTCAGTGACTGCTCCACCGCCCCATCTTCCTCTTCATCTAAAACCCTCGACGACCTAGGCCTCTCCTCCTCCTGCCGCGAGGTCACCGATCTTTCGCTTTCTTCCGTGCAGTCCACAATTGAAGATCTTATCCTCAAAATTGCTCGTTCCATTCTTTCCGGCAATGGTTTTGCATTTGATGTTCCTTCTCGATCGGCGGCCAATCAATTGTACGTTCCGGAGCTCGATCGCATCGTTTTGAAAGACAAAACCGCCATTCGACCCTACGCCAATATTTCCACTGTTCGGAAGTCCACCATTACTACTCGCATTCTTCAGCTTGTCCACCAACTTTGCATAAAAAACATCCATGTGACCAAGCGAGATCTGTTCTACACGGATGTAAAGCTCTTCCAGGATCAGACTCAGTCGGATTCTGTGCTTGATGATGTTTCTTGCATTCTCGGCTGTACTCGGTCGAGTCTCAATGTGATTGCTGCGGAGAAAGGAGTGGTTGTGGGAAGGCTCATTTTCAGTGACAATGGGGATATGATCGATTGCACTAAAATGGGCATGGGCGGAAAAGCGATTCCGCCAAATATTGATAGAGTTGGAGATATGCAGAGTGATGCATTGTTTATCCTCTTAGTTGAGAAAGATGCCGCGTATATTAGGTTAGCTGAGGATAGGTTTTACAATCGCTTTCCATGTATTATTGTGACCGCTAAGGGACAACCAGATGTGGCCACGAGATTGTTCTTGAGGAAGATGAAAATGGAGCTGAAGCTCCCAGTGCTTGCGCTTGTAGACAGTGATCCATATGGGTTGAAGATTCTATCTGTCTATGGATGTGGATCAAAGAATATGTCTTATGACAGTGCTAATTTGACTACTCCTGATATCAAGTGGCTAGGAATAAGGCCGAGTGATTTGGATAAATATAATATACCGGAGCAGTGCAGGTTACCTATGACAGAGCAGGATATTAAGACTGGGAAAGATCTGTTAGAGGAAGATTTTGTTAAAAAGAATCCAGGTTGGGTTGAAGAGCTCACTTTAATGGTGAAGACGAAGCAGAAGGCTGAGATTCAGGCATTGAGCTCATTCGGGTTTCAGTATCTGTCGGAGGTTTATCTGCCATTGAAGCTGCAGCAGAAGGATTGGCTGTGA

Protein sequence

MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLSSSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKDKTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLDDVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDIKTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL

Homology

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BLAST of CmaCh03G004680 vs. ExPASy Swiss-Prot
Match: Q9LZ03 (DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.0e-200
Identity = 359/429 (83.68%), Postives = 394/429 (91.84%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCST-------APSSSSSK- 60
           MADK+KR+RS+   D   +LPFK+ L+SD +I ++LK +   S          SSSSSK 
Sbjct: 1   MADKKKRKRSK--DDEAEELPFKSILESDDVITELLKSYISSSIKAAAGAGGASSSSSKP 60

Query: 61  -TLDDLGLSSSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVP 120
            TL DL LSSSCREV DLSLSSVQ+ IE +I++IARSIL+G+GF+F VPSR+A+NQLYVP
Sbjct: 61  LTLADLSLSSSCREVADLSLSSVQTEIETVIVQIARSILAGDGFSFSVPSRAASNQLYVP 120

Query: 121 ELDRIVLKDKTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQD 180
           ELDRIVLKDK+ +RP+A++S+VRK+TITTRIL L+HQLC++NIHVTKRDLFYTDVKLFQD
Sbjct: 121 ELDRIVLKDKSTLRPFASVSSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQD 180

Query: 181 QTQSDSVLDDVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPN 240
           QTQSD+VLDDVSC+LGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPN
Sbjct: 181 QTQSDAVLDDVSCMLGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPN 240

Query: 241 IDRVGDMQSDALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKME 300
           IDRVGDMQSDA+FILLVEKDAAY+RLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKME
Sbjct: 241 IDRVGDMQSDAMFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKME 300

Query: 301 LKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQC 360
           LKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKY IPEQC
Sbjct: 301 LKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQC 360

Query: 361 RLPMTEQDIKTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLP 420
           RLPMTEQDIKTGKD+LEEDFVKKNPGWVEEL LMVKTKQKAEIQALSSFGFQYLSEVYLP
Sbjct: 361 RLPMTEQDIKTGKDMLEEDFVKKNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLP 420

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BLAST of CmaCh03G004680 vs. ExPASy Swiss-Prot
Match: A2XFC1 (DNA topoisomerase 6 subunit A3 OS=Oryza sativa subsp. indica OX=39946 GN=TOP6A3 PE=1 SV=2)

HSP 1 Score: 565.5 bits (1456), Expect = 5.2e-160
Identity = 288/409 (70.42%), Postives = 345/409 (84.35%), Query Frame = 0


Query: 23  KNSLKSDSIILQILKEFSDC--------STAPSSSSSKTL---DDLGLSSSCREVTDLSL 82
           ++ L+ D+ IL  L+  +          S++ SSS+SK L   DD   ++S   V D   
Sbjct: 36  RSKLRPDASILATLRSLASACSKSKPAGSSSSSSSASKALAAEDD--PAASYIVVADQDS 95

Query: 83  SSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKDKTAIRPYANIS 142
           +SV S I  L+L  ARSILSG GF+F VPSR+A+NQ+Y+P+LDRIVL  + + RP+AN++
Sbjct: 96  ASVTSRINRLVLAAARSILSGRGFSFAVPSRAASNQVYLPDLDRIVLVRRESARPFANVA 155

Query: 143 TVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLDDVSCILGCTRS 202
           T RK+TIT R+L LVH +  + IHVTKRDLFYTDVKLF DQ QSD+VLDDVSC+LGCTRS
Sbjct: 156 TARKATITARVLSLVHAVLRRGIHVTKRDLFYTDVKLFGDQAQSDAVLDDVSCMLGCTRS 215

Query: 203 SLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFILLVEKD 262
           SL+V+A+EKGVVVGRL F+D+GD IDCT+MG+GGKAIPPNIDRV  ++SDALFILLVEKD
Sbjct: 216 SLHVVASEKGVVVGRLTFADDGDRIDCTRMGVGGKAIPPNIDRVSGIESDALFILLVEKD 275

Query: 263 AAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKILS 322
           AA++RLAEDRFYNRFPCII+TAKGQPDVATRLFLR++K+ELKLPVLALVDSDPYGLKILS
Sbjct: 276 AAFMRLAEDRFYNRFPCIILTAKGQPDVATRLFLRRLKVELKLPVLALVDSDPYGLKILS 335

Query: 323 VYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDIKTGKDLLEEDF 382
           VY CGSKNMSYDSANLTTPDIKWLG+RPSDLDKY +PEQCRLPMT+ DIK GK+LLEEDF
Sbjct: 336 VYMCGSKNMSYDSANLTTPDIKWLGVRPSDLDKYRVPEQCRLPMTDHDIKVGKELLEEDF 395

Query: 383 VKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 421
           VK+N GWV+EL  M++T+QKAEIQALSSFGFQYL+EVYLPLKLQQ+DW+
Sbjct: 396 VKQNEGWVKELETMLRTRQKAEIQALSSFGFQYLTEVYLPLKLQQQDWI 442

[Close]

BLAST of CmaCh03G004680 vs. ExPASy Swiss-Prot
Match: Q0DSV9 (Topoisomerase 6 subunit A3 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-3 PE=2 SV=2)

HSP 1 Score: 565.5 bits (1456), Expect = 5.2e-160
Identity = 288/409 (70.42%), Postives = 345/409 (84.35%), Query Frame = 0


Query: 23  KNSLKSDSIILQILKEFSDC--------STAPSSSSSKTL---DDLGLSSSCREVTDLSL 82
           ++ L+ D+ IL  L+  +          S++ SSS+SK L   DD   ++S   V D   
Sbjct: 36  RSKLRPDASILATLRSLASACSKSKPAGSSSSSSSASKALAAEDD--PAASYIVVADQDS 95

Query: 83  SSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKDKTAIRPYANIS 142
           +SV S I  L+L  ARSILSG GF+F VPSR+A+NQ+Y+P+LDRIVL  + + RP+AN++
Sbjct: 96  ASVTSRINRLVLAAARSILSGRGFSFAVPSRAASNQVYLPDLDRIVLVRRESARPFANVA 155

Query: 143 TVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLDDVSCILGCTRS 202
           T RK+TIT R+L LVH +  + IHVTKRDLFYTDVKLF DQ QSD+VLDDVSC+LGCTRS
Sbjct: 156 TARKATITARVLSLVHAVLRRGIHVTKRDLFYTDVKLFGDQAQSDAVLDDVSCMLGCTRS 215

Query: 203 SLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFILLVEKD 262
           SL+V+A+EKGVVVGRL F+D+GD IDCT+MG+GGKAIPPNIDRV  ++SDALFILLVEKD
Sbjct: 216 SLHVVASEKGVVVGRLTFADDGDRIDCTRMGVGGKAIPPNIDRVSGIESDALFILLVEKD 275

Query: 263 AAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKILS 322
           AA++RLAEDRFYNRFPCII+TAKGQPDVATRLFLR++K+ELKLPVLALVDSDPYGLKILS
Sbjct: 276 AAFMRLAEDRFYNRFPCIILTAKGQPDVATRLFLRRLKVELKLPVLALVDSDPYGLKILS 335

Query: 323 VYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDIKTGKDLLEEDF 382
           VY CGSKNMSYDSANLTTPDIKWLG+RPSDLDKY +PEQCRLPMT+ DIK GK+LLEEDF
Sbjct: 336 VYMCGSKNMSYDSANLTTPDIKWLGVRPSDLDKYRVPEQCRLPMTDHDIKVGKELLEEDF 395

Query: 383 VKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 421
           VK+N GWV+EL  M++T+QKAEIQALSSFGFQYL+EVYLPLKLQQ+DW+
Sbjct: 396 VKQNEGWVKELETMLRTRQKAEIQALSSFGFQYLTEVYLPLKLQQQDWI 442

[Close]

BLAST of CmaCh03G004680 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.3e-65
Identity = 130/355 (36.62%), Postives = 221/355 (62.25%), Query Frame = 0


Query: 70  SLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKDKTAIRPYAN 129
           S S V ++IE++I  I  S+      AF + +RS+   +   +   + +      R   +
Sbjct: 43  SSSEVLASIENIIQDIITSLARNEAPAFTIDNRSSWENIKFEDSVGLQMVSHCTTRKIKS 102

Query: 130 IS--TVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLDDVSCILG 189
            S  + +K ++  +IL ++++L   N + TKRD++YTD +LF +QT  D++++D+SC+L 
Sbjct: 103 DSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDISCMLK 162

Query: 190 CTRSSLNVIAAEKGVVVGRLIF-SDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFIL 249
            +R SL++++  KG++ G L +  ++G  ++CT  G    A+P NI  + ++ +DA F+L
Sbjct: 163 VSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCT-CGATAVAVPSNIQGIRNLVTDAKFVL 222

Query: 250 LVEKDAAYIRLAEDRFYNRF-PCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPY 309
           +VEKDA + RL +D F N+  PCI++T KG PD+ TRL ++K+     +PV  LVD+DP+
Sbjct: 223 IVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLVDADPH 282

Query: 310 GLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDIKTGKD 369
           G++I+ +Y  GS +MS+++ +LT P I+WLG+ PSDL + N+P+   +P+T++D      
Sbjct: 283 GIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDSLIPLTKRDQMKLDS 342

Query: 370 LLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 421
           +L   +V   P W +E+ +M  +K KAEIQAL+     YLS VYLP KL+   W+
Sbjct: 343 ILRRPYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPNKLKFGGWI 396

[Close]

BLAST of CmaCh03G004680 vs. ExPASy Swiss-Prot
Match: Q9WTK8 (Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2)

HSP 1 Score: 250.8 bits (639), Expect = 2.8e-65
Identity = 133/355 (37.46%), Postives = 216/355 (60.85%), Query Frame = 0


Query: 70  SLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKDKTAIRPYAN 129
           S S V + IE++I  I +S+      AF + +RS+   +   +   + +  +   R   +
Sbjct: 43  SSSEVLTAIENIIQDIIKSLARNEVPAFTIDNRSSWENIMFDDSVGLRMIPQCTTRKIRS 102

Query: 130 IS--TVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLDDVSCILG 189
            S  +V+K  +  ++L ++++L   + + TKRD++YTD +LF +Q   DS +DD+SC+L 
Sbjct: 103 DSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYYTDSQLFGNQAAVDSAIDDISCMLK 162

Query: 190 CTRSSLNVIAAEKGVVVGRLIF-SDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFIL 249
             R SL+V++  KG++ G L +  ++G  + CT       A+P NI  +  + +DA F+L
Sbjct: 163 VPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCT-CSATATAVPTNIQGMQHLITDAKFLL 222

Query: 250 LVEKDAAYIRLAEDRFYNRF-PCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPY 309
           +VEKDA + RL +D F +R  PCI+VT KG PD+ TRL ++K+     +PV  LVD+DPY
Sbjct: 223 IVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLVDADPY 282

Query: 310 GLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDIKTGKD 369
           G++I+ +Y  GS +MS+++ NLT P I+WLG+ PSD+ + NIP+   +P+T+ D      
Sbjct: 283 GIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNIPKDSLIPLTKHDQMKLDS 342

Query: 370 LLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 421
           +L+  ++   P W +EL +M  +K KAEIQAL+     YLS VYLP KL+   W+
Sbjct: 343 ILKRPYITYQPLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLPNKLRFGGWI 396

[Close]

BLAST of CmaCh03G004680 vs. ExPASy TrEMBL
Match: A0A6J1IM23 (DNA topoisomerase 6 subunit A OS=Cucurbita maxima OX=3661 GN=LOC111477696 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.6e-233
Identity = 420/420 (100.00%), Postives = 420/420 (100.00%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. ExPASy TrEMBL
Match: A0A6J1GGP2 (DNA topoisomerase 6 subunit A OS=Cucurbita moschata OX=3662 GN=LOC111453780 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.1e-232
Identity = 418/420 (99.52%), Postives = 419/420 (99.76%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNPD EAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSELNPDGEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEEL+LMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELSLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. ExPASy TrEMBL
Match: A0A6J1CR74 (DNA topoisomerase 6 subunit A OS=Momordica charantia OX=3673 GN=LOC111013584 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 1.7e-227
Identity = 408/420 (97.14%), Postives = 413/420 (98.33%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNPD EAQLPFKN LK DS ILQI+KEF DCSTAPSSSSSKTL+DLGLS
Sbjct: 1   MADKRKRRRSELNPDDEAQLPFKNRLKPDSAILQIIKEFGDCSTAPSSSSSKTLEDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           S+CREVTDLSLSSVQSTIE LILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  STCREVTDLSLSSVQSTIESLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTA+RPYANISTVRKSTITTRILQLVHQLC+KNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTALRPYANISTVRKSTITTRILQLVHQLCMKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKY IPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. ExPASy TrEMBL
Match: A0A5A7TWM5 (DNA topoisomerase 6 subunit A OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6A PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.1e-225
Identity = 405/420 (96.43%), Postives = 410/420 (97.62%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNP+ EAQLPFKN LK+DS ILQILKE SD S   SSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSELNPEDEAQLPFKNKLKADSFILQILKELSDSSAVSSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQ+TIE +ILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQATIESVILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTA+RPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTALRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKY IPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. ExPASy TrEMBL
Match: A0A1S3BGY2 (DNA topoisomerase 6 subunit A OS=Cucumis melo OX=3656 GN=LOC103489716 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.1e-225
Identity = 405/420 (96.43%), Postives = 410/420 (97.62%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNP+ EAQLPFKN LK+DS ILQILKE SD S   SSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSELNPEDEAQLPFKNKLKADSFILQILKELSDSSAVSSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQ+TIE +ILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQATIESVILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTA+RPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTALRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKY IPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. NCBI nr
Match: XP_022977345.1 (DNA topoisomerase 6 subunit A [Cucurbita maxima])

HSP 1 Score: 818.1 bits (2112), Expect = 3.4e-233
Identity = 420/420 (100.00%), Postives = 420/420 (100.00%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. NCBI nr
Match: XP_023544835.1 (DNA topoisomerase 6 subunit A [Cucurbita pepo subsp. pepo] >XP_023544836.1 DNA topoisomerase 6 subunit A [Cucurbita pepo subsp. pepo] >XP_023544837.1 DNA topoisomerase 6 subunit A [Cucurbita pepo subsp. pepo] >XP_023544838.1 DNA topoisomerase 6 subunit A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 813.9 bits (2101), Expect = 6.4e-232
Identity = 418/420 (99.52%), Postives = 418/420 (99.52%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNPD E QLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSELNPDGEVQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. NCBI nr
Match: XP_022950775.1 (DNA topoisomerase 6 subunit A [Cucurbita moschata] >KAG6603699.1 DNA topoisomerase 6 subunit A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033876.1 DNA topoisomerase 6 subunit A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 813.9 bits (2101), Expect = 6.4e-232
Identity = 418/420 (99.52%), Postives = 419/420 (99.76%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNPD EAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSELNPDGEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEEL+LMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELSLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. NCBI nr
Match: XP_022143741.1 (DNA topoisomerase 6 subunit A [Momordica charantia])

HSP 1 Score: 798.1 bits (2060), Expect = 3.6e-227
Identity = 408/420 (97.14%), Postives = 413/420 (98.33%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSELNPD EAQLPFKN LK DS ILQI+KEF DCSTAPSSSSSKTL+DLGLS
Sbjct: 1   MADKRKRRRSELNPDDEAQLPFKNRLKPDSAILQIIKEFGDCSTAPSSSSSKTLEDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           S+CREVTDLSLSSVQSTIE LILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  STCREVTDLSLSSVQSTIESLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTA+RPYANISTVRKSTITTRILQLVHQLC+KNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTALRPYANISTVRKSTITTRILQLVHQLCMKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKY IPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. NCBI nr
Match: XP_038881252.1 (DNA topoisomerase 6 subunit A [Benincasa hispida])

HSP 1 Score: 793.5 bits (2048), Expect = 8.9e-226
Identity = 409/420 (97.38%), Postives = 411/420 (97.86%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCSTAPSSSSSKTLDDLGLS 60
           MADKRKRRRSE NPD EAQLPFK SLK DS ILQILKEFSD STA SSSSSKTLDDLGLS
Sbjct: 1   MADKRKRRRSEPNPDGEAQLPFKKSLKPDSFILQILKEFSDSSTASSSSSSKTLDDLGLS 60

Query: 61  SSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120
           SSCREVTDLSLSSVQSTIE +ILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD
Sbjct: 61  SSCREVTDLSLSSVQSTIESVILKIARSILSGNGFAFDVPSRSAANQLYVPELDRIVLKD 120

Query: 121 KTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180
           KTA+RPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD
Sbjct: 121 KTALRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQDQTQSDSVLD 180

Query: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240
           DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS
Sbjct: 181 DVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQS 240

Query: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300
           DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV
Sbjct: 241 DALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALV 300

Query: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQCRLPMTEQDI 360
           DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKY IPEQCRLPMTEQDI
Sbjct: 301 DSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDI 360

Query: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420
           KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL
Sbjct: 361 KTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQKDWL 420

[Close]

BLAST of CmaCh03G004680 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 700.7 bits (1807), Expect = 7.3e-202
Identity = 359/429 (83.68%), Postives = 394/429 (91.84%), Query Frame = 0


Query: 1   MADKRKRRRSELNPDVEAQLPFKNSLKSDSIILQILKEFSDCST-------APSSSSSK- 60
           MADK+KR+RS+   D   +LPFK+ L+SD +I ++LK +   S          SSSSSK 
Sbjct: 1   MADKKKRKRSK--DDEAEELPFKSILESDDVITELLKSYISSSIKAAAGAGGASSSSSKP 60

Query: 61  -TLDDLGLSSSCREVTDLSLSSVQSTIEDLILKIARSILSGNGFAFDVPSRSAANQLYVP 120
            TL DL LSSSCREV DLSLSSVQ+ IE +I++IARSIL+G+GF+F VPSR+A+NQLYVP
Sbjct: 61  LTLADLSLSSSCREVADLSLSSVQTEIETVIVQIARSILAGDGFSFSVPSRAASNQLYVP 120

Query: 121 ELDRIVLKDKTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRDLFYTDVKLFQD 180
           ELDRIVLKDK+ +RP+A++S+VRK+TITTRIL L+HQLC++NIHVTKRDLFYTDVKLFQD
Sbjct: 121 ELDRIVLKDKSTLRPFASVSSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQD 180

Query: 181 QTQSDSVLDDVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPN 240
           QTQSD+VLDDVSC+LGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPN
Sbjct: 181 QTQSDAVLDDVSCMLGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPN 240

Query: 241 IDRVGDMQSDALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKME 300
           IDRVGDMQSDA+FILLVEKDAAY+RLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKME
Sbjct: 241 IDRVGDMQSDAMFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKME 300

Query: 301 LKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYNIPEQC 360
           LKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKY IPEQC
Sbjct: 301 LKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQC 360

Query: 361 RLPMTEQDIKTGKDLLEEDFVKKNPGWVEELTLMVKTKQKAEIQALSSFGFQYLSEVYLP 420
           RLPMTEQDIKTGKD+LEEDFVKKNPGWVEEL LMVKTKQKAEIQALSSFGFQYLSEVYLP
Sbjct: 361 RLPMTEQDIKTGKDMLEEDFVKKNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLP 420

[Close]

BLAST of CmaCh03G004680 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-57
Identity = 124/314 (39.49%), Postives = 184/314 (58.60%), Query Frame = 0


Query: 111 PELDRIVLKDKTAIRPYANISTVRKSTIT------TRILQLVHQLCIKNIHVTKRDLFYT 170
           PE D +   DK   +    I T++K   T       R+L +V QL  +N H +KRD++Y 
Sbjct: 48  PEADCLCSSDKPKGQ---EIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYM 107

Query: 171 DVKLFQDQTQSDSVLDDVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMG 230
               F+ Q+  D  + D+  +  C+R +LNV++   G+V+G L F + G   DC      
Sbjct: 108 HPSAFKAQSIVDRAIGDICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNT 167

Query: 231 GKAIPPNIDRVGDMQSDALFILLVEKDAAYIRLAEDRFYNRFPCIIVTAKGQPDVATRLF 290
              +P  ++ V D+ S A +IL+VEK+  + RLA D F     CI++T +G PDV+TR F
Sbjct: 168 AYPVPVLVEEVEDIVSLAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRF 227

Query: 291 LRKMKMELKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDK 350
           LR +  +L LPV  LVD DPYG +IL+ Y  GS  M+YD  +L  PD+KWLG  PSD + 
Sbjct: 228 LRLLMEKLHLPVHCLVDCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEV 287

Query: 351 YNIPEQCRLPMTEQDIKTGKDLLEEDFVKKN-PGWVEELTLMVKTKQKAEIQALSSFGFQ 410
           Y++P+QC LP+TE+D K  + +L   ++K+  P W  EL  M+K   K EI+ALS     
Sbjct: 288 YSVPKQCLLPLTEEDKKRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLS 347

Query: 411 YLSEVYLPLKLQQK 418
           +LSEVY+P K++++
Sbjct: 348 FLSEVYIPSKIRRE 358

[Close]

BLAST of CmaCh03G004680 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 190.3 bits (482), Expect = 3.2e-48
Identity = 111/328 (33.84%), Postives = 187/328 (57.01%), Query Frame = 0


Query: 101 SRSAANQLYVPELDRIVLKDKTAIRPYANISTVRKSTITTRILQLVHQLCIKNIHVTKRD 160
           S S  N+  + ++  I L          N  T +      +++++  Q+ ++   VT+R+
Sbjct: 62  SNSCINKGILTDVSYIFLSTSFTKSSLTNAKTAKAFVRVWKVMEICFQILLQEKRVTQRE 121

Query: 161 LFY----TDVKLFQDQTQSDSVLDDVSCILGCTRSSLNVIAAEKGVVVGRLIFSDNG-DM 220
           LFY         F  Q + +  + DV  +L C+R SL ++A+ +G+V GRL   + G + 
Sbjct: 122 LFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIMASSRGLVAGRLFLQEPGKEA 181

Query: 221 IDCTKMGMGGKAIPPNIDRVGD--MQSDALFILLVEKDAAYIRLAEDRFYNRFPCIIVTA 280
           +DC+  G  G AI  +++ + +  M++DA +I++VEK A + RL EDR +N  PC+ +TA
Sbjct: 182 VDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAIFHRLVEDRVFNHIPCVFITA 241

Query: 281 KGQPDVATRLFLRKMKMEL-KLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDI 340
           KG PD+ATR FL +M      LP+L LVD +P GL IL  +  GS  M  ++      ++
Sbjct: 242 KGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAILCTFKFGSIGMGLEAYRYAC-NV 301

Query: 341 KWLGIRPSDLDKYNIPEQCRLPMTEQDIKTGKDLLEEDFVKKNPGWVEELTLMVKTKQKA 400
           KW+G+R  DL+   IPE+  +P+  +D +  K LL    +++N  ++EEL+LMV+T ++A
Sbjct: 302 KWIGLRGDDLNL--IPEESLVPLKPKDSQIAKSLLSSKILQEN--YIEELSLMVQTGKRA 361

Query: 401 EIQALSSFGFQYLSEVYLPLKLQQKDWL 421
           EI+AL   G+ YL + Y+  K+ Q  ++
Sbjct: 362 EIEALYCHGYNYLGK-YIATKIVQGKYI 383

The following BLAST results are available for this feature:

BLAST of CmaCh03G004680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZ03	1.0e-200	83.68	DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1	[more]
A2XFC1	5.2e-160	70.42	DNA topoisomerase 6 subunit A3 OS=Oryza sativa subsp. indica OX=39946 GN=TOP6A3 ...	[more]
Q0DSV9	5.2e-160	70.42	Topoisomerase 6 subunit A3 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-3 P...	[more]
Q9Y5K1	1.3e-65	36.62	Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1	[more]
Q9WTK8	2.8e-65	37.46	Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2	[more]

BLAST of CmaCh03G004680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IM23	1.6e-233	100.00	DNA topoisomerase 6 subunit A OS=Cucurbita maxima OX=3661 GN=LOC111477696 PE=3 S...	[more]
A0A6J1GGP2	3.1e-232	99.52	DNA topoisomerase 6 subunit A OS=Cucurbita moschata OX=3662 GN=LOC111453780 PE=3...	[more]
A0A6J1CR74	1.7e-227	97.14	DNA topoisomerase 6 subunit A OS=Momordica charantia OX=3673 GN=LOC111013584 PE=...	[more]
A0A5A7TWM5	8.1e-225	96.43	DNA topoisomerase 6 subunit A OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6A PE...	[more]
A0A1S3BGY2	8.1e-225	96.43	DNA topoisomerase 6 subunit A OS=Cucumis melo OX=3656 GN=LOC103489716 PE=3 SV=1	[more]

BLAST of CmaCh03G004680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022977345.1	3.4e-233	100.00	DNA topoisomerase 6 subunit A [Cucurbita maxima]	[more]
XP_023544835.1	6.4e-232	99.52	DNA topoisomerase 6 subunit A [Cucurbita pepo subsp. pepo] >XP_023544836.1 DNA t...	[more]
XP_022950775.1	6.4e-232	99.52	DNA topoisomerase 6 subunit A [Cucurbita moschata] >KAG6603699.1 DNA topoisomera...	[more]
XP_022143741.1	3.6e-227	97.14	DNA topoisomerase 6 subunit A [Momordica charantia]	[more]
XP_038881252.1	8.9e-226	97.38	DNA topoisomerase 6 subunit A [Benincasa hispida]	[more]

BLAST of CmaCh03G004680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02820.1	7.3e-202	83.68	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT3G13170.1	1.3e-57	39.49	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT1G63990.1	3.2e-48	33.84	sporulation 11-2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002815	Spo11/DNA topoisomerase VI subunit A	PRINTS	PR01550	TOP6AFAMILY	coord: 269..290 score: 49.43 coord: 158..169 score: 53.65 coord: 172..189 score: 39.24 coord: 301..317 score: 55.15 coord: 190..205 score: 44.92 coord: 245..262 score: 51.04
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 46..420
IPR004085	DNA topoisomerase VI, subunit A	PRINTS	PR01552	TPISMRASE6A	coord: 391..414 score: 42.19 coord: 166..183 score: 32.64 coord: 275..295 score: 47.62 coord: 100..120 score: 30.95 coord: 219..237 score: 34.87
IPR004085	DNA topoisomerase VI, subunit A	HAMAP	MF_00132	Top6A	coord: 68..420 score: 36.503216
IPR013049	Spo11/DNA topoisomerase VI, subunit A, N-terminal	PFAM	PF04406	TP6A_N	coord: 134..195 e-value: 2.2E-20 score: 72.4
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 60..198 e-value: 4.6E-39 score: 135.6
None	No IPR available	GENE3D	3.40.1360.10		coord: 199..420 e-value: 2.4E-78 score: 264.7
None	No IPR available	PANTHER	PTHR10848:SF4	DNA TOPOISOMERASE 6 SUBUNIT A	coord: 46..420
IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain	CDD	cd00223	TOPRIM_TopoIIB_SPO	coord: 244..404 e-value: 1.57902E-72 score: 222.9
IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily	SUPERFAMILY	56726	DNA topoisomerase IV, alpha subunit	coord: 134..420

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G004680.1	CmaCh03G004680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006265	DNA topological change
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0000706	meiotic DNA double-strand break processing
biological_process	GO:0007131	reciprocal meiotic recombination
biological_process	GO:0006259	DNA metabolic process
cellular_component	GO:0009330	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
cellular_component	GO:0000228	nuclear chromosome
cellular_component	GO:0005694	chromosome
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0003824	catalytic activity