CmaCh03G004500 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G004500
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPectate lyase
LocationCma_Chr03: 4652415 .. 4656307 (-)
RNA-Seq ExpressionCmaCh03G004500
SyntenyCmaCh03G004500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTTATCCAGAGAGGTGAAAGGGCTCAAAGCCCCCCTCCACCATAACCGACCATTCAACTTTCACTGCAACAATTTTTACAGGCAAGCTTTGATCCGATCCGATCCAATCCCATTAAAAGCTCCAAACGCCTTCTGTTAATGGGGAAAGCCCACGATCACCGCTAGCGTCTCAACGATGGCGTAAATGCCTCTCGACCCACCAACGCTCATTCTCTCCCCAATAAATATAACCCATTTCTCGGAACTCTGCCTCGACGGTCAAGATGGAATCCTTGCCCTACGCCCACGTTGATTCGAGCCTTCGAGCCCTCGCCGGTCAGGCCGAGGGCTTTGGCCGTTCTGCCATTGGTGGTCTTTATGGCTCTGTTTATTGTGTCACTAATTTAGCAGGTCGTATTTTCTTTATCCCATTTTTATGCATTTGCTTTGATTGTTTGATGTTGGGATTGGAATTCAATTAGTCGTTGAGACTTTCTTACATTAGGCACATGAATGATCTCAAGTTCTCTCTTGTTTGGGTTTGTTTTTGGTTATTTTCCCTTTTTTGTATGCTTTGTTGGGCGAGTGGGGGGAAGAACTGAAGTTCTTTTTGAGAAATTTAGCAATCATAAGGGCTAGGGAGTTGGATTTTGGATTCTGCATTCTCTACCTATGCCTTGTTGAGATGTGTTCATGAGTTTGACAATTGCTTCCGAATGTTATATTGTGAGATTCCATGTCGATTGGAGAGGGGAACGGGTATGGAAATCTCTCCCTAGAGGACATGTTTTTAAAACCTTGAGGGGAAGCCCAGAAGGGGAAGCCCAAAAAAGACAATATTTGCTAGGGGTGGGGCTTGGGTTGTTACAAATGGTATTAGAGCCATCAAAGTTGAGCTTCAAAAGGGGAGGATTGTGAGATCCCACATTATTTGGAGAGGGGAACGGAACATTCCTTACAAGAGTATGGAAACCTCACTGTGATATCTCACCTTGGGGAACGAAACATTTCTTATAAGGGTATGGAAACCTCTTTGTGATATCCCACATTGGTTGGAGAAGGGAACGAAACATTCTTTATAAGGGTGTGGAAACTTCTCTCTAGTAGACGCATTTTAAAAACCTTGAGGAGAAACTTAGAAGAGAAAACCCAATGAGGAGAAACTTAGAAGAGAAAACCTAAAGTGGACGAAACATTCTTTATAATGGTGTGGAAACCTCTTTCTAGCAAACGCGTTTTAAAAACCTTGAGGGAAAACCCAGAAGAGAAAAACCAAAGATATATCTACTAACGATGGGTTTGGGATGTTACAAATGGTATCAAAACCATACACCGGGCGATGTGCCAACGAGGACACTGGGCCCTGAAGGGTGTGAATTGTGAGATCCTACATCGGTTGGTGAGAGGAACGAAACATTCTTTATAAAGGTGTGAAAACCTCTCCCCAATATACGCATTTTAAAAACCTTGAAGGAAAACTCAGAAGAGGAAACCTAAAGAGGACAATATCTGCTAGCGGTAGGTTTGGGTTGTTACACTCTCCCTAGCGGATGCATTGGGTATCTGCTAGTGGTGGACTTGAATTGTTACATATACACAGTATATAATACTTCTATTTAGTTTTGTCTTGCAGTTCTCTAAGAAATCTTTACATCTGTACTTTCTCATTGTTGTAGATGATGGTCCGGGATCGCTTAGGTTCGGGTGTCGTCTTAAAGAGCCCCTTTGGATTATCTTTGAAGTTTCAGGCACAATTCACTTGTCGACATTTCTTAGCGTGTCATCATATAAAACTGTAGATGGTCGTGGACAAAGGATAAAATTTACAGGAAAGGGATTGAGGCTGAAGGAATGTGAGCATGTGATCATTTGCAATTTGGAGTTTGAAGGTGGTAGAGGTCATGATGTTGATGGAGTTCAAATCAAACCCAATTCAAAGCACATATGGATAGATCGTTGCAGCCTCCGAGACTATGATGATGGGTTGATTGATATCACCAGAGGAAGCACAGATATCACTATTTCTAGGTGATAGCAAGCTTCATCTAAGAGCTTTCTTGGTGCCTTAACAATCTAACAATGTGTTTATCTATAGGTGCCACTTCTCACAGCATGACAAGACGATGCTCATCGGAGCCGATCCATCGCACGTCGGCGATCGATGCATTCGTGTCACTATTCACCACTGTTTTTTCGATGGTACTCGGCAACGGCATCCTCGTGTTAGATTTGCTCAAGTGCATCTATACAATAATTACACCAGAAACTGGGGGATCTATGCTGTCTGTGCTAGTGTAGAATCACAGGTCTTGTTGATTTCACTTACATTTTAGTTTGAACTTCTCCAAAGATCACTGTGATTTAAGTTGGTTGCTTGCATTTTGAATCAGATATACTCTCAGTGCAACATATACGAAGCAGGAGAGAAAAAGGTGGCATTTAGATACCTCGCTGAAAAGGTAAATTTGTTCTTTTTCATTCTCTATGATTTGAGTTGAAACGTAGATTATGTCTTTAACTTAAAAGGGTAAGCATCTCACAATCTTAAACATTAGATAGGGCGTATGTGAGATCGTATATCAGTTGGAGAGGGGAACGAAGCATTCTTTATAAGGGTGTGAAAGTCTCTCCCTCATAGACGTGTTTTAAAAACCTTGAAGAGAAGCCCGAAAGTGAAAGCCCAAAGAGGATAATACTTGTTAGCAGTGGGCTTGGGCTGTTACAAATGGTATTAGAGCCAGGTTCCGAGCGGTGTGCTAGTGAGGACGCTTAGCCCGGAAGGGGGTGGACAATGGACGGTGTGCTAACGAGGACGCTGAGCCCGGAAGGGGGTGGACATTGGGCGGTGTGTTAGCGAGGACGCTTGACCCCGAAGGTGGTGGATGTTTATAACAACCCAAGCCTACCACTAACATATATTATCCTATTTGGGTTTTTTCTTTCGGGTTTCCCCTTGATGTTTTAAAACATGTTTACTAGAGAGAGGTTTCCACACCCTTCTCCAACCGATATGGGATCTTACATGATGTATCCCTTCCGGACTAACTACTAAGACCCACATGATGAAATCACTTACACACATCATGTATCCGTTCCCCCTCTCCTAACCTCCTTTCTGCGATATATGATAACAATCAACGGCCTATTGTCTCTCTCTCTCTACTTTTGTCTGTCCATACATCTTTCTCACTGTCTCTGTAAGATCCCACATCAGTTAAAGATGGAACAAAACATTTCTTATAAAAGTGTGGAAACCTCACCCTAACCACTAACAGACTCGTTTTAAAACCACGAGCCTGACACGAGATGTAACAAGTCAAAACATACAATATTTACAAGAGGTGGACTTGGACTATTACAAGCTTTATTCATGTTTTCATTGCGCACATTCGCGACACTCGTCCTGCATGTCTCTGCCAATGATTGGTTCATCCTTAAAAAGGACACATTCAAAAACGAAATGCCAATAACGTTACTAAACTGGAACCATGGTTGATATATATCTGATATTATGTTCAGGCAGCAGACAAGGAAGAGCCATCAAGTGGGTGCATATGGTCCGACGGAGACTTATTTGTAAATGGCACTCAAAGCGGGCTGTCGGCGCCAACCACTGGACACAGCTCGTTTCATCCCTCACAACATTACACTGCCTGGACAGTGGAACCTCCAACGGATGCTCTAAAACAGATCCTCCAACTTTGTACTGGATGCCAAAACATCCAAACCCCCACTGATCTGCCTCTTCCACCTTAGAAAATTATACAAATGATTCGAATCGTAATTTTGGTCCCTCTACTTTTATCCATTTTCATTTTCAAATTGTAACTCGTATTGTTTGAGTTTGGTAGTCTATATTAGTCGAGTTATTAAGTCATACGAACACCCCTAATCTACAAGTATAAGCACGT

mRNA sequence

ACCTTTATCCAGAGAGGTGAAAGGGCTCAAAGCCCCCCTCCACCATAACCGACCATTCAACTTTCACTGCAACAATTTTTACAGGCAAGCTTTGATCCGATCCGATCCAATCCCATTAAAAGCTCCAAACGCCTTCTGTTAATGGGGAAAGCCCACGATCACCGCTAGCGTCTCAACGATGGCGTAAATGCCTCTCGACCCACCAACGCTCATTCTCTCCCCAATAAATATAACCCATTTCTCGGAACTCTGCCTCGACGGTCAAGATGGAATCCTTGCCCTACGCCCACGTTGATTCGAGCCTTCGAGCCCTCGCCGGTCAGGCCGAGGGCTTTGGCCGTTCTGCCATTGGTGGTCTTTATGGCTCTGTTTATTGTGTCACTAATTTAGCAGATGATGGTCCGGGATCGCTTAGGTTCGGGTGTCGTCTTAAAGAGCCCCTTTGGATTATCTTTGAAGTTTCAGGCACAATTCACTTGTCGACATTTCTTAGCGTGTCATCATATAAAACTGTAGATGGTCGTGGACAAAGGATAAAATTTACAGGAAAGGGATTGAGGCTGAAGGAATGTGAGCATGTGATCATTTGCAATTTGGAGTTTGAAGGTGGTAGAGGTCATGATGTTGATGGAGTTCAAATCAAACCCAATTCAAAGCACATATGGATAGATCGTTGCAGCCTCCGAGACTATGATGATGGGTTGATTGATATCACCAGAGGAAGCACAGATATCACTATTTCTAGGTGCCACTTCTCACAGCATGACAAGACGATGCTCATCGGAGCCGATCCATCGCACGTCGGCGATCGATGCATTCGTGTCACTATTCACCACTGTTTTTTCGATGGTACTCGGCAACGGCATCCTCGTGTTAGATTTGCTCAAGTGCATCTATACAATAATTACACCAGAAACTGGGGGATCTATGCTGTCTGTGCTAGTGTAGAATCACAGATATACTCTCAGTGCAACATATACGAAGCAGGAGAGAAAAAGGTGGCATTTAGATACCTCGCTGAAAAGGCAGCAGACAAGGAAGAGCCATCAAGTGGGTGCATATGGTCCGACGGAGACTTATTTGTAAATGGCACTCAAAGCGGGCTGTCGGCGCCAACCACTGGACACAGCTCGTTTCATCCCTCACAACATTACACTGCCTGGACAGTGGAACCTCCAACGGATGCTCTAAAACAGATCCTCCAACTTTGTACTGGATGCCAAAACATCCAAACCCCCACTGATCTGCCTCTTCCACCTTAGAAAATTATACAAATGATTCGAATCGTAATTTTGGTCCCTCTACTTTTATCCATTTTCATTTTCAAATTGTAACTCGTATTGTTTGAGTTTGGTAGTCTATATTAGTCGAGTTATTAAGTCATACGAACACCCCTAATCTACAAGTATAAGCACGT

Coding sequence (CDS)

ATGGAATCCTTGCCCTACGCCCACGTTGATTCGAGCCTTCGAGCCCTCGCCGGTCAGGCCGAGGGCTTTGGCCGTTCTGCCATTGGTGGTCTTTATGGCTCTGTTTATTGTGTCACTAATTTAGCAGATGATGGTCCGGGATCGCTTAGGTTCGGGTGTCGTCTTAAAGAGCCCCTTTGGATTATCTTTGAAGTTTCAGGCACAATTCACTTGTCGACATTTCTTAGCGTGTCATCATATAAAACTGTAGATGGTCGTGGACAAAGGATAAAATTTACAGGAAAGGGATTGAGGCTGAAGGAATGTGAGCATGTGATCATTTGCAATTTGGAGTTTGAAGGTGGTAGAGGTCATGATGTTGATGGAGTTCAAATCAAACCCAATTCAAAGCACATATGGATAGATCGTTGCAGCCTCCGAGACTATGATGATGGGTTGATTGATATCACCAGAGGAAGCACAGATATCACTATTTCTAGGTGCCACTTCTCACAGCATGACAAGACGATGCTCATCGGAGCCGATCCATCGCACGTCGGCGATCGATGCATTCGTGTCACTATTCACCACTGTTTTTTCGATGGTACTCGGCAACGGCATCCTCGTGTTAGATTTGCTCAAGTGCATCTATACAATAATTACACCAGAAACTGGGGGATCTATGCTGTCTGTGCTAGTGTAGAATCACAGATATACTCTCAGTGCAACATATACGAAGCAGGAGAGAAAAAGGTGGCATTTAGATACCTCGCTGAAAAGGCAGCAGACAAGGAAGAGCCATCAAGTGGGTGCATATGGTCCGACGGAGACTTATTTGTAAATGGCACTCAAAGCGGGCTGTCGGCGCCAACCACTGGACACAGCTCGTTTCATCCCTCACAACATTACACTGCCTGGACAGTGGAACCTCCAACGGATGCTCTAAAACAGATCCTCCAACTTTGTACTGGATGCCAAAACATCCAAACCCCCACTGATCTGCCTCTTCCACCTTAG

Protein sequence

MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLWIIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDVDGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVGDRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEAGEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWTVEPPTDALKQILQLCTGCQNIQTPTDLPLPP
Homology
BLAST of CmaCh03G004500 vs. ExPASy Swiss-Prot
Match: Q9FM66 (Putative pectate lyase 21 OS=Arabidopsis thaliana OX=3702 GN=At5g55720 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.3e-45
Identity = 111/280 (39.64%), Postives = 147/280 (52.50%), Query Frame = 0

Query: 16  LAGQAEGFGRSAIGGLYGSVYCVTNLADDG-----PGSLRFGCRLKEPLWIIFEVSGTIH 75
           LA  A GFGR +IGG  G  Y VT+  DD      PG+LR+     +PLWIIF+    I 
Sbjct: 68  LADCAVGFGRDSIGGRAGEFYTVTDSGDDNPLNPTPGTLRYAATQDQPLWIIFDRDMVIQ 127

Query: 76  LSTFLSVSSYKTVDGRGQRIKFT-GKGLRLKECEHVIICNLEFEG------------GRG 135
           L   L V+SYKT+DGRG  ++   G  L L +  ++II NL                G  
Sbjct: 128 LKQDLQVASYKTIDGRGNNVQIAYGPCLTLYKVSNIIINNLYIHDCVPVKRNALSSLGGY 187

Query: 136 HDVDGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPS 195
            D DG+ I   S+ IWID C+L    DGLID   GSTDITIS  +   H++ ML+G    
Sbjct: 188 SDGDGISIF-ESRDIWIDHCTLEKCYDGLIDAVNGSTDITISNSYMLNHNEVMLLGHSDE 247

Query: 196 HVGDRCIRVTIHHCFF-DGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCN 255
           + GDR +RVTI   +F +G  QR PR R    H+ NN  R+W +YA+  S    I+SQ N
Sbjct: 248 YSGDRDMRVTIAFNYFGEGLVQRMPRCRHGYFHIVNNIYRDWKMYAIGGSANPTIFSQGN 307

Query: 256 IYEAGEKKVAFRYLAEKAADKEEPSSGCIW-SDGDLFVNG 276
           ++ A   +        ++AD +E      W S+GD  VNG
Sbjct: 308 VFIASNNQFTKEVTKRESADGDEEWKEWNWKSEGDEMVNG 346

BLAST of CmaCh03G004500 vs. ExPASy Swiss-Prot
Match: Q9C8G4 (Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.7e-45
Identity = 108/277 (38.99%), Postives = 159/277 (57.40%), Query Frame = 0

Query: 14  RALAGQAEGFGRSAIGGLYGSVYCVTNLADD----GPGSLRFGCRLKEPLWIIFEVSGTI 73
           +ALA  A G+G++AIGG  G +Y VTN +D+     PG+LR+     +PLWI F     I
Sbjct: 53  QALAHCAVGYGKAAIGGKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKPLWITFARDMVI 112

Query: 74  HLSTFLSVSSYKTVDGRGQRIKF-TGKGLRLKECEHVIICNLEFEGGR----GHDVDGVQ 133
            L + L ++SYKT+DGRG +++   G  LR+++ +HVII  +     +    G D DG++
Sbjct: 113 VLKSQLMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADPNGMDGDGIR 172

Query: 134 IKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVGDRCI 193
           +   S H+WID C L    DGLID+   ST +TIS  +F+QHDK ML+G D S++GD+ +
Sbjct: 173 VF-QSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDM 232

Query: 194 RVTI-HHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEAGEK 253
           RVTI  + F  G  +R PRVR    H+ NN    W +YA+  S    I+S+ N + A EK
Sbjct: 233 RVTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEK 292

Query: 254 KVAFRYLAEKAADKEEPSSGCIW-SDGDLFVNGTQSG 280
           + + +      A  +  S    W +  D+F+NG   G
Sbjct: 293 RSSKQVTKRMMAGPD--SKRWKWGTSRDVFMNGAFFG 326

BLAST of CmaCh03G004500 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 176.4 bits (446), Expect = 5.3e-43
Identity = 115/310 (37.10%), Postives = 161/310 (51.94%), Query Frame = 0

Query: 10  DSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDG-----PGSLRFGCRLKEPLWIIFE 69
           +++ + LA  A GFG++AIGG  G +Y VT+ A+D      PG+LR+    +EPLWIIF+
Sbjct: 77  ETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPLWIIFK 136

Query: 70  VSGTIHLSTFLSVSSYKTVDGRGQRIKFT-GKGLRLKECEHVIICNLE-----------F 129
               I L   L ++S+KT+DGRG  +  T G  L++    ++II  +             
Sbjct: 137 RDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPGSGGMI 196

Query: 130 EGGRGH-------DVDGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQ 189
           + G  H       D D V I    KH+WID CSL + DDGLID   GST ITIS  H + 
Sbjct: 197 KDGPHHTGWWMQSDGDAVAIF-GGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNNHMTH 256

Query: 190 HDKTMLIGADPSHVGDRCIRVTI-HHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVC 249
           HDK ML+G   S+  D+ ++VTI  + F +G  QR PR R    H+ NN   +W +YA+ 
Sbjct: 257 HDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIG 316

Query: 250 ASVESQIYSQCNIYEAGEKKVAFRYLAEKAADKEEPSSGCIW------SDGDLFVNGT-- 286
            S    IYSQ N + A   +        K   K E +    W      S+GD+ +NG   
Sbjct: 317 GSASPTIYSQGNRFLAPNTRF------NKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYF 376

BLAST of CmaCh03G004500 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.5e-42
Identity = 113/301 (37.54%), Postives = 156/301 (51.83%), Query Frame = 0

Query: 16  LAGQAEGFGRSAIGGLYGSVYCVTNLADDG-----PGSLRFGCRLKEPLWIIFEVSGTIH 75
           LA  + GFG   +GG  G +Y VT+ +D+      PG+LR+G   +EPLWI+F  +  I 
Sbjct: 99  LADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGVIQEEPLWIVFSSNMLIR 158

Query: 76  LSTFLSVSSYKTVDGRGQRIKFTGKG-LRLKECEHVIICNLE---------FEGGRGHDV 135
           L   L ++SYKT+DGRG  +  TG G L L+  +H+II NL          FE     D 
Sbjct: 159 LKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHIYDCKPSAGFEKRGRSDG 218

Query: 136 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 195
           DG+ I   S+ IW+D CS+    DGLID   GST ITIS  +F+ HD+ ML+G D ++  
Sbjct: 219 DGISIF-GSQKIWVDHCSMSHCTDGLIDAVMGSTAITISNNYFTHHDEVMLLGHDDNYAP 278

Query: 196 DRCIRVTI-HHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYE 255
           D  ++VTI  + F  G  QR PR R   +H+ NN    W +YA+  S    I SQ N Y 
Sbjct: 279 DTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNNDFTEWKMYAIGGSGNPTINSQGNRYS 338

Query: 256 AGEKKVAFRYLAEKAADKEEPSSGCIW---SDGDLFVNG-----TQSGLSAPTTGHSSFH 293
           A     A      K  D ++      W   ++GDL  NG     +  G+S+  +  SS  
Sbjct: 339 APSDPSAKE--VTKRVDSKDDGEWSNWNWRTEGDLMENGAFFVASGEGMSSMYSKASSVD 396

BLAST of CmaCh03G004500 vs. ExPASy Swiss-Prot
Match: O65388 (Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 173.3 bits (438), Expect = 4.5e-42
Identity = 115/320 (35.94%), Postives = 161/320 (50.31%), Query Frame = 0

Query: 11  SSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADD----GPGSLRFGCRLKEPLWIIFEVS 70
           S+ RALA  A GFG+SA+GG YGS+Y VTN +DD     PG+LR+     +PLWI F   
Sbjct: 51  SNRRALADCAVGFGKSAVGGKYGSIYVVTNPSDDPENPRPGTLRYAVIQSKPLWITFARD 110

Query: 71  GTIHLSTFLSVSSYKTVDGRGQRIKFT-GKGLRLKECEHVIICNLEF------EGGR--- 130
             I L   L ++SYKT+DGRG +++   G  + ++   HVII  +        + GR   
Sbjct: 111 MVIVLRNELIMNSYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRS 170

Query: 131 ---------GHDVDGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHD 190
                    G D D + I  +S HIWID C      DGLID+   ST +TIS  +F+QHD
Sbjct: 171 SPTHVGSRKGSDGDAIAIF-DSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHD 230

Query: 191 KTMLIGADPSHVGDRCIRVTI-HHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCAS 250
           K ML+G + ++V D+ +RVTI  + F  G  +R PRVR    H+ NN    W +YA+  S
Sbjct: 231 KVMLLGHNDNNVEDKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGS 290

Query: 251 VESQIYSQCNIYEAGEKKVAFRYLAEKAADKEEPSSGCIW------SDGDLFVNG----- 296
            +  I+S+ N + A +          K    +   SG  W      +  D+F NG     
Sbjct: 291 ADPTIFSEGNYFVASDD-------PSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYFVP 350

BLAST of CmaCh03G004500 vs. ExPASy TrEMBL
Match: A0A6J1IPY9 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478392 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 7.9e-199
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW
Sbjct: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG
Sbjct: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT
Sbjct: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQLCTGCQNIQTPTDLPLPP
Sbjct: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 331

BLAST of CmaCh03G004500 vs. ExPASy TrEMBL
Match: A0A6J1GEQ3 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111453514 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.2e-191
Identity = 322/331 (97.28%), Postives = 324/331 (97.89%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCR+KEPLW
Sbjct: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRMKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG
Sbjct: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAFRYL EKAADKEEPSSGCI SDGDLFVN TQSGLSAPT G SSFHPSQHYTAWT
Sbjct: 241 GEKKVAFRYLTEKAADKEEPSSGCIRSDGDLFVNDTQSGLSAPTAGPSSFHPSQHYTAWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQLCTGCQNI +PTDLPL P
Sbjct: 301 VEPPTDALKQILQLCTGCQNIPSPTDLPLLP 331

BLAST of CmaCh03G004500 vs. ExPASy TrEMBL
Match: A0A5A7TWJ1 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001550 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.0e-178
Identity = 296/331 (89.43%), Postives = 310/331 (93.66%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPY HVDSSLRALA QAEGFGRSAIGGL+GSVYCVTNL+DDGPGSLRFGCR+KEPLW
Sbjct: 46  MESLPYPHVDSSLRALAAQAEGFGRSAIGGLHGSVYCVTNLSDDGPGSLRFGCRMKEPLW 105

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTI LS+++SVSSYKTVDGRGQRIK TGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 106 IIFEVSGTIDLSSYVSVSSYKTVDGRGQRIKLTGKGLRLKECEHVIICNLEFEGGRGHDV 165

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DG+QIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRC+FS HDKTMLIGADPSH+G
Sbjct: 166 DGIQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCYFSHHDKTMLIGADPSHIG 225

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVR+AQVHLYNNYTRNWGIYAVCASVES+IYSQCNIYEA
Sbjct: 226 DRCIRVTIHHCFFDGTRQRHPRVRYAQVHLYNNYTRNWGIYAVCASVESKIYSQCNIYEA 285

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAF+YL EKA DKEEPSSG IWSDGDLFV GTQSGL APT  H+ FHPS+HYT WT
Sbjct: 286 GEKKVAFKYLTEKATDKEEPSSGTIWSDGDLFVKGTQSGLLAPTAEHNMFHPSEHYTNWT 345

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEP TDALK ILQ CTGCQNI  PTD PL P
Sbjct: 346 VEPATDALKHILQHCTGCQNIPRPTDQPLVP 376

BLAST of CmaCh03G004500 vs. ExPASy TrEMBL
Match: A0A1S3BGV0 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489868 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.0e-178
Identity = 296/331 (89.43%), Postives = 310/331 (93.66%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPY HVDSSLRALA QAEGFGRSAIGGL+GSVYCVTNL+DDGPGSLRFGCR+KEPLW
Sbjct: 46  MESLPYPHVDSSLRALAAQAEGFGRSAIGGLHGSVYCVTNLSDDGPGSLRFGCRMKEPLW 105

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTI LS+++SVSSYKTVDGRGQRIK TGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 106 IIFEVSGTIDLSSYVSVSSYKTVDGRGQRIKLTGKGLRLKECEHVIICNLEFEGGRGHDV 165

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DG+QIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRC+FS HDKTMLIGADPSH+G
Sbjct: 166 DGIQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCYFSHHDKTMLIGADPSHIG 225

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVR+AQVHLYNNYTRNWGIYAVCASVES+IYSQCNIYEA
Sbjct: 226 DRCIRVTIHHCFFDGTRQRHPRVRYAQVHLYNNYTRNWGIYAVCASVESKIYSQCNIYEA 285

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAF+YL EKA DKEEPSSG IWSDGDLFV GTQSGL APT  H+ FHPS+HYT WT
Sbjct: 286 GEKKVAFKYLTEKATDKEEPSSGTIWSDGDLFVKGTQSGLLAPTAEHNMFHPSEHYTNWT 345

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEP TDALK ILQ CTGCQNI  PTD PL P
Sbjct: 346 VEPATDALKHILQHCTGCQNIPRPTDQPLVP 376

BLAST of CmaCh03G004500 vs. ExPASy TrEMBL
Match: A0A6J1HFI4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463488 PE=3 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 2.9e-177
Identity = 292/331 (88.22%), Postives = 312/331 (94.26%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYA VDS LRALAGQAEGFGR+AIGGL+GSVYCVTNL DDGPGSLRFGCR+KEPLW
Sbjct: 46  MESLPYALVDSRLRALAGQAEGFGRAAIGGLHGSVYCVTNLEDDGPGSLRFGCRMKEPLW 105

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLS++L+VSS+KTVDGRGQRIK TGKGLRLKECEHVIICNLEFEGGRGHD+
Sbjct: 106 IIFEVSGTIHLSSYLNVSSFKTVDGRGQRIKLTGKGLRLKECEHVIICNLEFEGGRGHDI 165

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DG+QIKPNS+HIWIDRCSL+DYDDGLIDITRGSTDITISRC FS+HDKTMLIGADPSHVG
Sbjct: 166 DGIQIKPNSRHIWIDRCSLQDYDDGLIDITRGSTDITISRCRFSRHDKTMLIGADPSHVG 225

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVR+ +VHLYNNYTRNWGIYAVCASVESQI+SQCNIYEA
Sbjct: 226 DRCIRVTIHHCFFDGTRQRHPRVRYGKVHLYNNYTRNWGIYAVCASVESQIHSQCNIYEA 285

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           G+KKVAF+YL EKA DKEEPSSGCIWS+GDLFV+GTQSGL A T G S FHPS HY+AWT
Sbjct: 286 GQKKVAFKYLTEKATDKEEPSSGCIWSEGDLFVSGTQSGLLAATAGQSMFHPSDHYSAWT 345

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQ CTGCQNI  P DLPL P
Sbjct: 346 VEPPTDALKQILQHCTGCQNIPRPCDLPLVP 376

BLAST of CmaCh03G004500 vs. NCBI nr
Match: XP_022978390.1 (probable pectate lyase 4 [Cucurbita maxima])

HSP 1 Score: 702.6 bits (1812), Expect = 1.6e-198
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW
Sbjct: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG
Sbjct: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT
Sbjct: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQLCTGCQNIQTPTDLPLPP
Sbjct: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 331

BLAST of CmaCh03G004500 vs. NCBI nr
Match: KAG7033859.1 (putative pectate lyase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 688.3 bits (1775), Expect = 3.2e-194
Identity = 325/331 (98.19%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCR+KEPLW
Sbjct: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRMKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG
Sbjct: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAFRYL EKAADKEEPSSGCIWSDGDLFVN TQSGLSAPTTG SSFHPSQHYTAWT
Sbjct: 241 GEKKVAFRYLTEKAADKEEPSSGCIWSDGDLFVNDTQSGLSAPTTGPSSFHPSQHYTAWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQLCTGCQNIQ+PTDLPL P
Sbjct: 301 VEPPTDALKQILQLCTGCQNIQSPTDLPLLP 331

BLAST of CmaCh03G004500 vs. NCBI nr
Match: XP_023544911.1 (probable pectate lyase 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 681.4 bits (1757), Expect = 3.9e-192
Identity = 323/331 (97.58%), Postives = 325/331 (98.19%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLR GCR+KEPLW
Sbjct: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRSGCRMKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG
Sbjct: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAFRYL EKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPT G SSFHPSQHYTAWT
Sbjct: 241 GEKKVAFRYLTEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTAGPSSFHPSQHYTAWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQLCTG QNIQ+PTDLPL P
Sbjct: 301 VEPPTDALKQILQLCTGRQNIQSPTDLPLLP 331

BLAST of CmaCh03G004500 vs. NCBI nr
Match: KAG6603682.1 (putative pectate lyase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 680.6 bits (1755), Expect = 6.6e-192
Identity = 322/331 (97.28%), Postives = 324/331 (97.89%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCR+KEPLW
Sbjct: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRMKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DGVQIKPNSKHIWIDRCSLRD DDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG
Sbjct: 121 DGVQIKPNSKHIWIDRCSLRDNDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAFRYL EKAADKEEPSSGCIWSDGDLFVN TQSGLSAPT G SSFHP QHYTAWT
Sbjct: 241 GEKKVAFRYLTEKAADKEEPSSGCIWSDGDLFVNDTQSGLSAPTAGPSSFHPLQHYTAWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQLCTGCQNIQ+PTDLPL P
Sbjct: 301 VEPPTDALKQILQLCTGCQNIQSPTDLPLLP 331

BLAST of CmaCh03G004500 vs. NCBI nr
Match: XP_022950402.1 (probable pectate lyase 4 [Cucurbita moschata])

HSP 1 Score: 678.7 bits (1750), Expect = 2.5e-191
Identity = 322/331 (97.28%), Postives = 324/331 (97.89%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCR+KEPLW
Sbjct: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRMKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV
Sbjct: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG
Sbjct: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           GEKKVAFRYL EKAADKEEPSSGCI SDGDLFVN TQSGLSAPT G SSFHPSQHYTAWT
Sbjct: 241 GEKKVAFRYLTEKAADKEEPSSGCIRSDGDLFVNDTQSGLSAPTAGPSSFHPSQHYTAWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLPP 332
           VEPPTDALKQILQLCTGCQNI +PTDLPL P
Sbjct: 301 VEPPTDALKQILQLCTGCQNIPSPTDLPLLP 331

BLAST of CmaCh03G004500 vs. TAIR 10
Match: AT3G55140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-152
Identity = 245/330 (74.24%), Postives = 284/330 (86.06%), Query Frame = 0

Query: 1   MESLPYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLW 60
           M SLPYA  D SLRALAG+AEGFGR A+GGL+G +Y VT+LADDGPG+LR G R KEPLW
Sbjct: 1   MTSLPYADADCSLRALAGRAEGFGRFAVGGLHGDLYVVTSLADDGPGTLREGGRRKEPLW 60

Query: 61  IIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDV 120
           I+F VSGTI+L+++LSVSSYKT+DGRGQRIK TGKG+RLKECEH+IICNLEFEGGRGHDV
Sbjct: 61  IVFAVSGTINLNSYLSVSSYKTIDGRGQRIKLTGKGIRLKECEHIIICNLEFEGGRGHDV 120

Query: 121 DGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVG 180
           DG+QIKP S+HIWIDRCSLRDYDDGLIDITR STDIT+SRC+F+QHDKTMLIGADPSHV 
Sbjct: 121 DGIQIKPKSRHIWIDRCSLRDYDDGLIDITRQSTDITVSRCYFAQHDKTMLIGADPSHVE 180

Query: 181 DRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEA 240
           DRCIRVTIHHCFFDGTRQR PR+RF +VHLYNNYTRNWGIYAVCASVE+Q++SQCNIYEA
Sbjct: 181 DRCIRVTIHHCFFDGTRQRQPRLRFGKVHLYNNYTRNWGIYAVCASVEAQVFSQCNIYEA 240

Query: 241 GEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWT 300
           G KK  F Y +EKAADKEE  +G + S+ DLF+NG Q  L    +    FHPS+HY  WT
Sbjct: 241 GVKKKTFEYYSEKAADKEEARTGLVRSENDLFLNGAQPSLMTGASEECVFHPSEHYPTWT 300

Query: 301 VEPPTDALKQILQLCTGCQNIQTPTDLPLP 331
           VEPP++ LKQI+Q+CTG Q++  P+D  +P
Sbjct: 301 VEPPSETLKQIMQICTGWQSLSRPSDHGVP 330

BLAST of CmaCh03G004500 vs. TAIR 10
Match: AT3G09540.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-136
Identity = 228/324 (70.37%), Postives = 268/324 (82.72%), Query Frame = 0

Query: 5   PYAHVDSSLRALAGQAEGFGRSAIGGLYGSVYCVTNLADDGPGSLRFGCRLKEPLWIIFE 64
           PY HVDSSLR+LAG+AEGFGR+A+GGL G +  VT+LAD+GPGSLR  C+  EPLWI+F+
Sbjct: 52  PYCHVDSSLRSLAGKAEGFGRAAVGGLNGPICHVTSLADEGPGSLREACKRPEPLWIVFD 111

Query: 65  VSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLKECEHVIICNLEFEGGRGHDVDGVQ 124
           VSGTI+LS+F++VSS+ TVDGRGQ++K TGKGLRLKECE+VIICNLEFEGG G D D +Q
Sbjct: 112 VSGTINLSSFVNVSSHTTVDGRGQKVKITGKGLRLKECENVIICNLEFEGGVGPDADAIQ 171

Query: 125 IKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVGDRCI 184
           IKP S +IWIDRCSL++Y DGLIDITR STDIT+SRCHF  H+KTMLIGAD SHV DRCI
Sbjct: 172 IKPKSHNIWIDRCSLKNYYDGLIDITRESTDITVSRCHFMNHNKTMLIGADTSHVTDRCI 231

Query: 185 RVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEAGEKK 244
           RVTIHHCFFDGTRQRHPRVRFA+VHL+NNYTR+W IYAV A VESQI+SQCNIYEAGEKK
Sbjct: 232 RVTIHHCFFDGTRQRHPRVRFAKVHLFNNYTRHWAIYAVGAGVESQIHSQCNIYEAGEKK 291

Query: 245 VAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGLSAPTTGHSSFHPSQHYTAWTVEPP 304
             F+Y+ EKAADKE+P +G + S+GDL +NG +S LS     +  F P QHY+ WTVE P
Sbjct: 292 TVFKYITEKAADKEKPGAGFVRSEGDLLLNGAKSCLSQGGERY-VFSPIQHYSEWTVESP 351

Query: 305 TDALKQILQLCTGCQNIQTPTDLP 329
           TD LK  L+  TG QN+  P D P
Sbjct: 352 TDILKNYLKHSTGWQNLPLPLDRP 374

BLAST of CmaCh03G004500 vs. TAIR 10
Match: AT3G55140.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 478.4 bits (1230), Expect = 4.6e-135
Identity = 215/290 (74.14%), Postives = 250/290 (86.21%), Query Frame = 0

Query: 41  LADDGPGSLRFGCRLKEPLWIIFEVSGTIHLSTFLSVSSYKTVDGRGQRIKFTGKGLRLK 100
           + DDGPG+LR G R KEPLWI+F VSGTI+L+++LSVSSYKT+DGRGQRIK TGKG+RLK
Sbjct: 17  ILDDGPGTLREGGRRKEPLWIVFAVSGTINLNSYLSVSSYKTIDGRGQRIKLTGKGIRLK 76

Query: 101 ECEHVIICNLEFEGGRGHDVDGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISR 160
           ECEH+IICNLEFEGGRGHDVDG+QIKP S+HIWIDRCSLRDYDDGLIDITR STDIT+SR
Sbjct: 77  ECEHIIICNLEFEGGRGHDVDGIQIKPKSRHIWIDRCSLRDYDDGLIDITRQSTDITVSR 136

Query: 161 CHFSQHDKTMLIGADPSHVGDRCIRVTIHHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGI 220
           C+F+QHDKTMLIGADPSHV DRCIRVTIHHCFFDGTRQR PR+RF +VHLYNNYTRNWGI
Sbjct: 137 CYFAQHDKTMLIGADPSHVEDRCIRVTIHHCFFDGTRQRQPRLRFGKVHLYNNYTRNWGI 196

Query: 221 YAVCASVESQIYSQCNIYEAGEKKVAFRYLAEKAADKEEPSSGCIWSDGDLFVNGTQSGL 280
           YAVCASVE+Q++SQCNIYEAG KK  F Y +EKAADKEE  +G + S+ DLF+NG Q  L
Sbjct: 197 YAVCASVEAQVFSQCNIYEAGVKKKTFEYYSEKAADKEEARTGLVRSENDLFLNGAQPSL 256

Query: 281 SAPTTGHSSFHPSQHYTAWTVEPPTDALKQILQLCTGCQNIQTPTDLPLP 331
               +    FHPS+HY  WTVEPP++ LKQI+Q+CTG Q++  P+D  +P
Sbjct: 257 MTGASEECVFHPSEHYPTWTVEPPSETLKQIMQICTGWQSLSRPSDHGVP 306

BLAST of CmaCh03G004500 vs. TAIR 10
Match: AT5G55720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-46
Identity = 111/280 (39.64%), Postives = 147/280 (52.50%), Query Frame = 0

Query: 16  LAGQAEGFGRSAIGGLYGSVYCVTNLADDG-----PGSLRFGCRLKEPLWIIFEVSGTIH 75
           LA  A GFGR +IGG  G  Y VT+  DD      PG+LR+     +PLWIIF+    I 
Sbjct: 68  LADCAVGFGRDSIGGRAGEFYTVTDSGDDNPLNPTPGTLRYAATQDQPLWIIFDRDMVIQ 127

Query: 76  LSTFLSVSSYKTVDGRGQRIKFT-GKGLRLKECEHVIICNLEFEG------------GRG 135
           L   L V+SYKT+DGRG  ++   G  L L +  ++II NL                G  
Sbjct: 128 LKQDLQVASYKTIDGRGNNVQIAYGPCLTLYKVSNIIINNLYIHDCVPVKRNALSSLGGY 187

Query: 136 HDVDGVQIKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPS 195
            D DG+ I   S+ IWID C+L    DGLID   GSTDITIS  +   H++ ML+G    
Sbjct: 188 SDGDGISIF-ESRDIWIDHCTLEKCYDGLIDAVNGSTDITISNSYMLNHNEVMLLGHSDE 247

Query: 196 HVGDRCIRVTIHHCFF-DGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCN 255
           + GDR +RVTI   +F +G  QR PR R    H+ NN  R+W +YA+  S    I+SQ N
Sbjct: 248 YSGDRDMRVTIAFNYFGEGLVQRMPRCRHGYFHIVNNIYRDWKMYAIGGSANPTIFSQGN 307

Query: 256 IYEAGEKKVAFRYLAEKAADKEEPSSGCIW-SDGDLFVNG 276
           ++ A   +        ++AD +E      W S+GD  VNG
Sbjct: 308 VFIASNNQFTKEVTKRESADGDEEWKEWNWKSEGDEMVNG 346

BLAST of CmaCh03G004500 vs. TAIR 10
Match: AT1G30350.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 4.0e-46
Identity = 108/277 (38.99%), Postives = 159/277 (57.40%), Query Frame = 0

Query: 14  RALAGQAEGFGRSAIGGLYGSVYCVTNLADD----GPGSLRFGCRLKEPLWIIFEVSGTI 73
           +ALA  A G+G++AIGG  G +Y VTN +D+     PG+LR+     +PLWI F     I
Sbjct: 53  QALAHCAVGYGKAAIGGKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKPLWITFARDMVI 112

Query: 74  HLSTFLSVSSYKTVDGRGQRIKF-TGKGLRLKECEHVIICNLEFEGGR----GHDVDGVQ 133
            L + L ++SYKT+DGRG +++   G  LR+++ +HVII  +     +    G D DG++
Sbjct: 113 VLKSQLMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADPNGMDGDGIR 172

Query: 134 IKPNSKHIWIDRCSLRDYDDGLIDITRGSTDITISRCHFSQHDKTMLIGADPSHVGDRCI 193
           +   S H+WID C L    DGLID+   ST +TIS  +F+QHDK ML+G D S++GD+ +
Sbjct: 173 VF-QSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDM 232

Query: 194 RVTI-HHCFFDGTRQRHPRVRFAQVHLYNNYTRNWGIYAVCASVESQIYSQCNIYEAGEK 253
           RVTI  + F  G  +R PRVR    H+ NN    W +YA+  S    I+S+ N + A EK
Sbjct: 233 RVTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEK 292

Query: 254 KVAFRYLAEKAADKEEPSSGCIW-SDGDLFVNGTQSG 280
           + + +      A  +  S    W +  D+F+NG   G
Sbjct: 293 RSSKQVTKRMMAGPD--SKRWKWGTSRDVFMNGAFFG 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FM663.3e-4539.64Putative pectate lyase 21 OS=Arabidopsis thaliana OX=3702 GN=At5g55720 PE=3 SV=1[more]
Q9C8G45.7e-4538.99Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1[more]
Q9LTZ05.3e-4337.10Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2[more]
Q93Z044.5e-4237.54Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1[more]
O653884.5e-4235.94Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1IPY97.9e-199100.00Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111478392 PE=3 SV=1[more]
A0A6J1GEQ31.2e-19197.28Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111453514 PE=3 SV=1[more]
A0A5A7TWJ12.0e-17889.43Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001550... [more]
A0A1S3BGV02.0e-17889.43Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489868 PE=3 SV=1[more]
A0A6J1HFI42.9e-17788.22Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463488 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022978390.11.6e-198100.00probable pectate lyase 4 [Cucurbita maxima][more]
KAG7033859.13.2e-19498.19putative pectate lyase 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023544911.13.9e-19297.58probable pectate lyase 4 [Cucurbita pepo subsp. pepo][more]
KAG6603682.16.6e-19297.28putative pectate lyase 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022950402.12.5e-19197.28probable pectate lyase 4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G55140.11.4e-15274.24Pectin lyase-like superfamily protein [more]
AT3G09540.13.2e-13670.37Pectin lyase-like superfamily protein [more]
AT3G55140.24.6e-13574.14Pectin lyase-like superfamily protein [more]
AT5G55720.12.4e-4639.64Pectin lyase-like superfamily protein [more]
AT1G30350.14.0e-4638.99Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 74..90
score: 49.58
coord: 19..36
score: 50.0
coord: 221..240
score: 38.57
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 31..244
e-value: 2.5E-33
score: 126.7
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 75..237
e-value: 6.0E-26
score: 91.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 6..311
e-value: 1.6E-90
score: 305.6
NoneNo IPR availablePANTHERPTHR31683:SF113PECTATE LYASEcoord: 1..324
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 1..324
IPR003308Integrase, N-terminal zinc-binding domainPROSITEPS50876ZF_INTEGRASEcoord: 278..319
score: 8.68783
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 17..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G004500.1CmaCh03G004500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0030570 pectate lyase activity
molecular_function GO:0008270 zinc ion binding