CmaCh03G003820 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G003820
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiontranscription factor MYB1
LocationCma_Chr03: 4358869 .. 4362322 (+)
RNA-Seq ExpressionCmaCh03G003820
SyntenyCmaCh03G003820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGTTGAAAGGAGAGCGGTTAAGTCAATACGGGGACTGAGTTCCGTGGACAGTTGCCGGTGACAATACTGTGATGGAGGACGCGGCCGCCACTGAGGTTGGTGCTTCCACCGCCGAAGGAGGAGGAGGAGGAGTAAATGAATGCGATGCCGTCGCTTCCGTCGCCGACTGCGGCAGTGGGGACGAGGCACTGCCGATGGTTGGCGAGGGGGAAACGACTGGTGGCCGTGGCGGCAAAGATAGAGTGAAGGGACCTTGGTCTCCTGAAGAGGATGCGATACTCAGCCGCCTTGTGAGCAAGTTCGGTGCGAGGAATTGGAGCTTGATTGCTCGGGGAATCGCTGGGCGATCGGGGAAGTCTTGTCGCCTTAGGTGGTGTAATCAGCTCGACCCTTCCGTGAAACGCAAGCCATTTACTGGTATTAGACTCTGTCCCTAATCACTTATTTGCTTGGCTGATTCTTTTATTTCTTTCATCGGCCCGATTCCGAATGTTTGATAGTGTTAAGCGTGTGGAGAAGAGAATTGAGTTATTTGCTTCTTGATGTAAAATTCTGATATTTAATCTTTGTTAATTCTACGTAATGTATATTCTTTTTTCCGAAGCCTAAATTGTTGTGTTGGACATTGCTCTATTCCTTAATTTCTCCTTCGTCTAGTTGGGACAATCATGTGATGCCATCATAAATATCAATGAAAATGGCATCTGTGATTTTGGTCTAGTTCAATGCGAGGTAAACCACTCAAGTTGGACAGGAACAAGTATGACGTGAATTATTTTCTCCTTATTTTGCTTTCTATTCTGGTAATCGTTTTAATGATCTAAATTTAGATATCAGCAGGTTCATTGTCCTGAAATTCAACATTCTCTGTGTATGTAGTAGTTTTTTAAAATGGTTCTCAAATGTTTAGACATAGGTATGAGATTAATATGATCTAGTTTGGTAGCGAATATTTAACTGAAAACTAAAGAAATGTGATCTAAACTTTATACTTAAATTGTTAATCGAACAAATGAAAATAGTGAAGCATACGGCATAAAGGACACACCTTTTCTCTTGTAGAGTTCCTATGTGATATGAGCATTTTTACTGTTATGTATCTGTATTTCGTATATCAATGCTTCCAATGGTGGGTAAAAATATGCATTGGGATAATTGATCCTTGTCGTGTTATGTTTTAATGGTGGGTAAAAATATGCAATTTTATTTCTGAAAGTGGCTTAAAGATCCATGTGTTTTCAGATGAGGAGGACAGGATCATTGTAGCAGCCCATGCTGTACATGGAAATAAATGGGCAGCAATTGCTAGACTTTTGCGTGGGAGAACAGATAATGCTATAAAGAACCACTGGAATTCCACTTTACGACGTCGATGCACAGAGCTTGAAAGAATCAAGTTAGAATCAGGGAATGTAGTGGATGATGCTAGTTTAGAAAAAACCAAGGGATCATCTGAAGAAACCCTTTCATGTGGGGATGTAAATTCCTTTAAATCCTTTGAAGGAAAAGACGCCAGCTCACGGGAGCATATGGACGATCAATTTGAAGACAAAGTCCCTATTGCTAGTGAGGGTCAATTTACTCATGAAGTAAAAGAACAGCCTACTCTTTACAGGCCCGTGGCTCGAGTAAGTGCTTTTAGTGTATATAACCCTTTGGATGACCAAGCATCTTTGAGGGCATTTATACGACCTGTCCCAATGCAGGGGCCATTGATTCAAGCATCAAAACCAGAAGTTGAAGCTAGCAAATTGCTTGAAGGTGTGTATGGTGATCGATCAGTGCCTCATCAATGTGGTCATGGTTGTTGCCAGAGTCATAACCAGGGATCTCCTCTAGACTCTTTATTAGGTCCAGAATTTGTGGACTTCTCAGAGCCTCCACTATCCTTTCCCAGTTTTGAACTAGCTGCAATTGCAACTGACATAAGTAACCTTGCTTGGCTGAAAAGTGGATTGGAGAATGGCAGTGTTAGAGCAATGGGAGATTCGGCTGGAAGATTAAATGGTTCTCAGATGCAAATGGGGCGTTTATGAGAGACATTCAGTTTGAAGTGACTAATAGTAGACTAGCCTCCAGCTGATATGATCGACGTCAACAAGGATTGTTTATGCGATCCCTTCATACTACAAGAATTGATCTGAATTATATATATGCGCACTGATGGGAGGCGTTTTCTTAAGCCCACTGGAGAAGCTTTTGGGGTTTACTGGATCTGTACTTTGCTTTGTGATTAGTTAGTTTTACCCTCTCCCACCTGTACAGCTCGTCTCTACTGCCAGGAATCGTGGATAAGAGGGAAGTAAAGCAACTCTCATGTCTAAGTTTCCTAAGTCCTTTTTTATCCTTTTGCTTTTTGTCTATGACCACAAGCTCGAGTTTGAAAACGGTTCGAACATGAATGAAACTTTTTGGTGTTAAGAGGGCCCAAACTTTGTCAGCAACTTAGTACCTGAAGTTTCTTGTAACATTGTTCCAGCTGATGAACATCCCTGGAATCTCCTTTGATGACTATATAATGGAAAGGTATGATTCTCCTACCTCAGTTGCATACTCTTGTTCTTAATAATGTCGCTGGGAACTGGGGATTCAGTGTAGCATTTTTTTTTTCCATCCTTAATGGATGTTGCTTATGTATAGTACGTATAGCCTGATTCTTCGGGTCATCTGAAATGCCTTCTCGACCTTAGAGCTTGTATGAAACTGAATTCATTATATATGAGAAAATGAATAAAATAATGAAAGCAAGAAATAAGTAAATGAAGCACAGATAACGGAGATGATCGATTGCATTCTGCATTTCTACTCTCATAACAGATATATATAGGTACTTGACGTGAATGGACATGTTCTTGAAATGGTAATTAATTCCATGAGCTTTTTGTGATGTGAAAAGCTTTAATACATCTATGAAGTCTGAATGCCAGTGCTCATCTGGTGCTCTAATGGCTACACAAGTGCAACTTTATAGATTATCTTAGAAGTCTTAACTTGACACGTTTTCCTTTTTTGGTTCTTTATAACTCATTTATTGGATATTTCATCTCTTTTCTGACTCTTTTTCGTTTGCCCCTTCCAATTTCCAACTCTCTATTTCAGGGCGACTCGAAGCTACAGTGTTCGTTGCCATAGAGATCTAAAGATAATTCTGACTACTCGTTAGTTTTAATTTAGCACAACTCTGAATCCTTTTTTATGTGATGCCTGACGCTTGCCAGATATAGATCACTATAATTGGTGAGGAATTTGAGTGCTTACTTGTGGGATAACATGATTGGTTATTTTATATTTTGTCTGTTCATGATAGAGGTGGTTTTTCCGTTCACCTATTCAATTTTATCAACATCTAAACCTACATTAATGAACAAGATGTTGAGGACTCTTGGGAGGGGTCTCACATTAGCTAATTAAAGGGATGATCATGGGTTATAAGTAAGAAATACATTTCCAT

mRNA sequence

GAGAGTTGAAAGGAGAGCGGTTAAGTCAATACGGGGACTGAGTTCCGTGGACAGTTGCCGGTGACAATACTGTGATGGAGGACGCGGCCGCCACTGAGGTTGGTGCTTCCACCGCCGAAGGAGGAGGAGGAGGAGTAAATGAATGCGATGCCGTCGCTTCCGTCGCCGACTGCGGCAGTGGGGACGAGGCACTGCCGATGGTTGGCGAGGGGGAAACGACTGGTGGCCGTGGCGGCAAAGATAGAGTGAAGGGACCTTGGTCTCCTGAAGAGGATGCGATACTCAGCCGCCTTGTGAGCAAGTTCGGTGCGAGGAATTGGAGCTTGATTGCTCGGGGAATCGCTGGGCGATCGGGGAAGTCTTGTCGCCTTAGGTGGTGTAATCAGCTCGACCCTTCCGTGAAACGCAAGCCATTTACTGATGAGGAGGACAGGATCATTGTAGCAGCCCATGCTGTACATGGAAATAAATGGGCAGCAATTGCTAGACTTTTGCGTGGGAGAACAGATAATGCTATAAAGAACCACTGGAATTCCACTTTACGACGTCGATGCACAGAGCTTGAAAGAATCAAGTTAGAATCAGGGAATGTAGTGGATGATGCTAGTTTAGAAAAAACCAAGGGATCATCTGAAGAAACCCTTTCATGTGGGGATGTAAATTCCTTTAAATCCTTTGAAGGAAAAGACGCCAGCTCACGGGAGCATATGGACGATCAATTTGAAGACAAAGTCCCTATTGCTAGTGAGGGTCAATTTACTCATGAAGTAAAAGAACAGCCTACTCTTTACAGGCCCGTGGCTCGAGTAAGTGCTTTTAGTGTATATAACCCTTTGGATGACCAAGCATCTTTGAGGGCATTTATACGACCTGTCCCAATGCAGGGGCCATTGATTCAAGCATCAAAACCAGAAGTTGAAGCTAGCAAATTGCTTGAAGGTGTGTATGGTGATCGATCAGTGCCTCATCAATGTGGTCATGGTTGTTGCCAGAGTCATAACCAGGGATCTCCTCTAGACTCTTTATTAGGTCCAGAATTTGTGGACTTCTCAGAGCCTCCACTATCCTTTCCCAGTTTTGAACTAGCTGCAATTGCAACTGACATAAGTAACCTTGCTTGGCTGAAAAGTGGATTGGAGAATGGCAGTGTTAGAGCAATGGGAGATTCGGCTGGAAGATTAAATGGTTCTCAGATGCAAATGGGGCGTTTATGAGAGACATTCAGTTTGAAGTGACTAATAGTAGACTAGCCTCCAGCTGATATGATCGACGTCAACAAGGATTGTTTATGCGATCCCTTCATACTACAAGAATTGATCTGAATTATATATATGCGCACTGATGGGAGGCGTTTTCTTAAGCCCACTGGAGAAGCTTTTGGGGTTTACTGGATCTGTACTTTGCTTTGTGATTAGTTAGTTTTACCCTCTCCCACCTGTACAGCTCGTCTCTACTGCCAGGAATCGTGGATAAGAGGGAAGTAAAGCAACTCTCATGTCTAAGTTTCCTAAGTCCTTTTTTATCCTTTTGCTTTTTGTCTATGACCACAAGCTCGAGTTTGAAAACGGTTCGAACATGAATGAAACTTTTTGGTGTTAAGAGGGCCCAAACTTTGTCAGCAACTTAGTACCTGAAGTTTCTTGTAACATTGTTCCAGCTGATGAACATCCCTGGAATCTCCTTTGATGACTATATAATGGAAAGGGCGACTCGAAGCTACAGTGTTCGTTGCCATAGAGATCTAAAGATAATTCTGACTACTCGTTAGTTTTAATTTAGCACAACTCTGAATCCTTTTTTATGTGATGCCTGACGCTTGCCAGATATAGATCACTATAATTGGTGAGGAATTTGAGTGCTTACTTGTGGGATAACATGATTGGTTATTTTATATTTTGTCTGTTCATGATAGAGGTGGTTTTTCCGTTCACCTATTCAATTTTATCAACATCTAAACCTACATTAATGAACAAGATGTTGAGGACTCTTGGGAGGGGTCTCACATTAGCTAATTAAAGGGATGATCATGGGTTATAAGTAAGAAATACATTTCCAT

Coding sequence (CDS)

ATGGAGGACGCGGCCGCCACTGAGGTTGGTGCTTCCACCGCCGAAGGAGGAGGAGGAGGAGTAAATGAATGCGATGCCGTCGCTTCCGTCGCCGACTGCGGCAGTGGGGACGAGGCACTGCCGATGGTTGGCGAGGGGGAAACGACTGGTGGCCGTGGCGGCAAAGATAGAGTGAAGGGACCTTGGTCTCCTGAAGAGGATGCGATACTCAGCCGCCTTGTGAGCAAGTTCGGTGCGAGGAATTGGAGCTTGATTGCTCGGGGAATCGCTGGGCGATCGGGGAAGTCTTGTCGCCTTAGGTGGTGTAATCAGCTCGACCCTTCCGTGAAACGCAAGCCATTTACTGATGAGGAGGACAGGATCATTGTAGCAGCCCATGCTGTACATGGAAATAAATGGGCAGCAATTGCTAGACTTTTGCGTGGGAGAACAGATAATGCTATAAAGAACCACTGGAATTCCACTTTACGACGTCGATGCACAGAGCTTGAAAGAATCAAGTTAGAATCAGGGAATGTAGTGGATGATGCTAGTTTAGAAAAAACCAAGGGATCATCTGAAGAAACCCTTTCATGTGGGGATGTAAATTCCTTTAAATCCTTTGAAGGAAAAGACGCCAGCTCACGGGAGCATATGGACGATCAATTTGAAGACAAAGTCCCTATTGCTAGTGAGGGTCAATTTACTCATGAAGTAAAAGAACAGCCTACTCTTTACAGGCCCGTGGCTCGAGTAAGTGCTTTTAGTGTATATAACCCTTTGGATGACCAAGCATCTTTGAGGGCATTTATACGACCTGTCCCAATGCAGGGGCCATTGATTCAAGCATCAAAACCAGAAGTTGAAGCTAGCAAATTGCTTGAAGGTGTGTATGGTGATCGATCAGTGCCTCATCAATGTGGTCATGGTTGTTGCCAGAGTCATAACCAGGGATCTCCTCTAGACTCTTTATTAGGTCCAGAATTTGTGGACTTCTCAGAGCCTCCACTATCCTTTCCCAGTTTTGAACTAGCTGCAATTGCAACTGACATAAGTAACCTTGCTTGGCTGAAAAGTGGATTGGAGAATGGCAGTGTTAGAGCAATGGGAGATTCGGCTGGAAGATTAAATGGTTCTCAGATGCAAATGGGGCGTTTATGA

Protein sequence

MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVRAMGDSAGRLNGSQMQMGRL
Homology
BLAST of CmaCh03G003820 vs. ExPASy Swiss-Prot
Match: Q42575 (Transcription factor MYB1 OS=Arabidopsis thaliana OX=3702 GN=MYB1 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 9.3e-84
Identity = 186/373 (49.87%), Postives = 239/373 (64.08%), Query Frame = 0

Query: 7   TEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKGPWSPEE 66
           + +G    +G GG V              G++A   VG    T GRG +DRVKGPWS EE
Sbjct: 21  SSIGRGDCDGDGGDV--------------GEDAAGFVG----TSGRGRRDRVKGPWSKEE 80

Query: 67  DAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDRIIVAAH 126
           D +LS LV + GARNWS IAR I GRSGKSCRLRWCNQL+P++ R  FT+ ED+ I+AAH
Sbjct: 81  DDVLSELVKRLGARNWSFIARSIPGRSGKSCRLRWCNQLNPNLIRNSFTEVEDQAIIAAH 140

Query: 127 AVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIK-LESGN-VVDDASLEKTK- 186
           A+HGNKWA IA+LL GRTDNAIKNHWNS LRRR  + E+ K + +G+ VVDD+  ++T  
Sbjct: 141 AIHGNKWAVIAKLLPGRTDNAIKNHWNSALRRRFIDFEKAKNIGTGSLVVDDSGFDRTTT 200

Query: 187 -GSSEETLS----CGDVNSFKSFEGKDA-SSREHMDDQFEDKVPIASEGQFTHEVKEQPT 246
             SSEETLS    C       S EGK+A +S E  ++Q  +K     EG    + K+ PT
Sbjct: 201 VASSEETLSSGGGCHVTTPIVSPEGKEATTSMEMSEEQCVEKT--NGEGISRQDDKDPPT 260

Query: 247 LYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 306
           L+RPV R+S+F+  N ++   S      P       +Q+SK +    +LLEG Y +R VP
Sbjct: 261 LFRPVPRLSSFNACNHMEGSPS------PHIQDQNQLQSSKQDAAMLRLLEGAYSERFVP 320

Query: 307 HQCGHGCCQSHNQGS-PLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLEN 366
             CG GCC ++  GS   +SLLGPEFVD+ + P +FPS ELAAIAT+I +LAWL+SGLE+
Sbjct: 321 QTCGGGCCSNNPDGSFQQESLLGPEFVDYLDSP-TFPSSELAAIATEIGSLAWLRSGLES 366

Query: 367 GSVRAMGDSAGRL 370
            SVR M D+ GRL
Sbjct: 381 SSVRVMEDAVGRL 366

BLAST of CmaCh03G003820 vs. ExPASy Swiss-Prot
Match: O04192 (Transcription factor MYB25 OS=Arabidopsis thaliana OX=3702 GN=MYB25 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.3e-65
Identity = 149/346 (43.06%), Postives = 204/346 (58.96%), Query Frame = 0

Query: 25  DAVASVADCGSGDEALPMVGEGE------TTGGRGGKDRVKGPWSPEEDAILSRLVSKFG 84
           + ++S   C S + A+    E E      +    GGK +VKGPW PE+D  L+RLV   G
Sbjct: 10  ELISSRNPCKSFENAIHKAVEAELAELAKSDANGGGKSKVKGPWLPEQDEALTRLVKMCG 69

Query: 85  ARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDRIIVAAHAVHGNKWAAIAR 144
            RNW+LI+RGI GRSGKSCRLRWCNQLDP +KRKPF+DEE+ +I++A AV GNKW+ IA+
Sbjct: 70  PRNWNLISRGIPGRSGKSCRLRWCNQLDPILKRKPFSDEEEHMIMSAQAVLGNKWSVIAK 129

Query: 145 LLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLEKTKGSSEETLSCGDVNSF 204
           LL GRTDNAIKNHWNS LRR+  E  +I L   N      + +   S    +S       
Sbjct: 130 LLPGRTDNAIKNHWNSNLRRKPAEQWKIPLLMSNT---EIVYQLYPSMVRRIS------- 189

Query: 205 KSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPT-------LYRPVARVSAFSVY 264
                 +AS +EH+    E++  + S+ +   E KE P        +YRPVAR+ AFSV 
Sbjct: 190 ------NASPKEHLPQ--EEETGVLSDDKMDDEAKEPPREQNSKTGVYRPVARMGAFSVC 249

Query: 265 NPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQCGHGCCQSHNQG 324
            P         ++   P +GPL+QAS+P+  A K L+ +  D  +P +CGHGCC +H   
Sbjct: 250 KP--------GYM--APCEGPLVQASRPDSLAGKFLQSLCYDPIIPSKCGHGCC-NHQDS 309

Query: 325 SPL--DSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLE 356
           + L   S+LG EFVD+ E   +    EL +I+ D++N AW++SG E
Sbjct: 310 TTLSSSSVLGSEFVDYEEHSSAELDKELISISNDLNNTAWIRSGKE 326

BLAST of CmaCh03G003820 vs. ExPASy Swiss-Prot
Match: O23160 (Transcription factor MYB73 OS=Arabidopsis thaliana OX=3702 GN=MYB73 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 5.7e-41
Identity = 75/110 (68.18%), Postives = 88/110 (80.00%), Query Frame = 0

Query: 52  RGGKDRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKR 111
           R   +R+KGPWSPEED +L RLV K G RNWSLI++ I GRSGKSCRLRWCNQL P V+ 
Sbjct: 6   RKNMERIKGPWSPEEDDLLQRLVQKHGPRNWSLISKSIPGRSGKSCRLRWCNQLSPEVEH 65

Query: 112 KPFTDEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCT 162
           + F+ EED  I+ AHA  GNKWA I+RLL GRTDNAIKNHWNSTL+R+C+
Sbjct: 66  RAFSQEEDETIIRAHARFGNKWATISRLLNGRTDNAIKNHWNSTLKRKCS 115

BLAST of CmaCh03G003820 vs. ExPASy Swiss-Prot
Match: Q9FDW1 (Transcription factor MYB44 OS=Arabidopsis thaliana OX=3702 GN=MYB44 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 7.4e-41
Identity = 74/105 (70.48%), Postives = 86/105 (81.90%), Query Frame = 0

Query: 56  DRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFT 115
           DR+KGPWSPEED  L RLV K+G RNW++I++ I GRSGKSCRLRWCNQL P V+ +PF+
Sbjct: 3   DRIKGPWSPEEDEQLRRLVVKYGPRNWTVISKSIPGRSGKSCRLRWCNQLSPQVEHRPFS 62

Query: 116 DEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRC 161
            EED  I  AHA  GNKWA IARLL GRTDNA+KNHWNSTL+R+C
Sbjct: 63  AEEDETIARAHAQFGNKWATIARLLNGRTDNAVKNHWNSTLKRKC 107

BLAST of CmaCh03G003820 vs. ExPASy Swiss-Prot
Match: Q9SN12 (Transcription factor MYB77 OS=Arabidopsis thaliana OX=3702 GN=MYB77 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.8e-40
Identity = 74/106 (69.81%), Postives = 85/106 (80.19%), Query Frame = 0

Query: 56  DRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFT 115
           DRVKGPWS EED  L R+V K+G RNWS I++ I GRSGKSCRLRWCNQL P V+ +PF+
Sbjct: 3   DRVKGPWSQEEDEQLRRMVEKYGPRNWSAISKSIPGRSGKSCRLRWCNQLSPEVEHRPFS 62

Query: 116 DEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCT 162
            EED  IV A A  GNKWA IARLL GRTDNA+KNHWNSTL+R+C+
Sbjct: 63  PEEDETIVTARAQFGNKWATIARLLNGRTDNAVKNHWNSTLKRKCS 108

BLAST of CmaCh03G003820 vs. ExPASy TrEMBL
Match: A0A6J1IM36 (transcription factor MYB1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478260 PE=4 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 2.6e-214
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60
           MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG
Sbjct: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60

Query: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120
           PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR
Sbjct: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120

Query: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180
           IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE
Sbjct: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180

Query: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240
           KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR
Sbjct: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240

Query: 241 PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC 300
           PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC
Sbjct: 241 PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC 300

Query: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 360
           GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR
Sbjct: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 360

Query: 361 AMGDSAGRLNGSQMQMGRL 380
           AMGDSAGRLNGSQMQMGRL
Sbjct: 361 AMGDSAGRLNGSQMQMGRL 379

BLAST of CmaCh03G003820 vs. ExPASy TrEMBL
Match: A0A6J1GGH8 (transcription factor MYB1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453721 PE=4 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 3.9e-210
Identity = 374/382 (97.91%), Postives = 376/382 (98.43%), Query Frame = 0

Query: 1   MEDAAATEVGASTAE---GGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDR 60
           MEDAAATEVGAS AE   GGGGGVNECDAVASVADCGSGDEA+PMVGEGETTGGRGGKDR
Sbjct: 1   MEDAAATEVGASAAEEGGGGGGGVNECDAVASVADCGSGDEAVPMVGEGETTGGRGGKDR 60

Query: 61  VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120
           VKGPWSPEEDAILSRLV KFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE
Sbjct: 61  VKGPWSPEEDAILSRLVGKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120

Query: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180
           EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA
Sbjct: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180

Query: 181 SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPT 240
           SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQ T
Sbjct: 181 SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQST 240

Query: 241 LYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300
           +YRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP
Sbjct: 241 IYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300

Query: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360
           HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG
Sbjct: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360

Query: 361 SVRAMGDSAGRLNGSQMQMGRL 380
           SVRAMGDSAGRLNGSQMQMGRL
Sbjct: 361 SVRAMGDSAGRLNGSQMQMGRL 382

BLAST of CmaCh03G003820 vs. ExPASy TrEMBL
Match: A0A6J1IKI5 (transcription factor MYB1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478260 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.0e-194
Identity = 353/379 (93.14%), Postives = 353/379 (93.14%), Query Frame = 0

Query: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60
           MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG
Sbjct: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60

Query: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120
           PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR
Sbjct: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120

Query: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180
           IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE
Sbjct: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180

Query: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240
           KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR
Sbjct: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240

Query: 241 PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC 300
           PVAR                          GPLIQASKPEVEASKLLEGVYGDRSVPHQC
Sbjct: 241 PVAR--------------------------GPLIQASKPEVEASKLLEGVYGDRSVPHQC 300

Query: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 360
           GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR
Sbjct: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 353

Query: 361 AMGDSAGRLNGSQMQMGRL 380
           AMGDSAGRLNGSQMQMGRL
Sbjct: 361 AMGDSAGRLNGSQMQMGRL 353

BLAST of CmaCh03G003820 vs. ExPASy TrEMBL
Match: A0A6J1DDF1 (transcription factor MYB1 OS=Momordica charantia OX=3673 GN=LOC111019477 PE=4 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 1.9e-193
Identity = 341/379 (89.97%), Postives = 358/379 (94.46%), Query Frame = 0

Query: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60
           ME  A  EV A+ AE   GGV+ CDAV SVADCGSGD+A+P+VGEGE +GGRGGKDRVKG
Sbjct: 1   MEGTAVAEVDATAAE---GGVDACDAVTSVADCGSGDDAVPIVGEGEASGGRGGKDRVKG 60

Query: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120
           PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR
Sbjct: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120

Query: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180
           IIVAAHA+HGNKWAAIARLL GRTDNAIKNHWNSTLRRRCTELERIKLESGN+VDDASLE
Sbjct: 121 IIVAAHAIHGNKWAAIARLLPGRTDNAIKNHWNSTLRRRCTELERIKLESGNMVDDASLE 180

Query: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240
           KTKGSSEETLSCGDVNSFKS E +DA SREHMDDQFEDKVPIA EGQF+HEVKEQPTL+R
Sbjct: 181 KTKGSSEETLSCGDVNSFKSLEARDACSREHMDDQFEDKVPIAIEGQFSHEVKEQPTLFR 240

Query: 241 PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC 300
           PVARVSAFSVYNPLD Q SLRAF+RPVPMQGPL+QASKP+VEASKLLEGVYGDRSVPHQC
Sbjct: 241 PVARVSAFSVYNPLDGQESLRAFLRPVPMQGPLVQASKPDVEASKLLEGVYGDRSVPHQC 300

Query: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 360
           GHGCC+SHNQGSP+DSLLGPEFVDFS+PP SFPSFELAAIATDISNLAWLKSGLENGSVR
Sbjct: 301 GHGCCESHNQGSPVDSLLGPEFVDFSDPPPSFPSFELAAIATDISNLAWLKSGLENGSVR 360

Query: 361 AMGDSAGRLNGSQMQMGRL 380
           AMGDSAGRLNG QMQMGRL
Sbjct: 361 AMGDSAGRLNGCQMQMGRL 376

BLAST of CmaCh03G003820 vs. ExPASy TrEMBL
Match: A0A1S3BKQ6 (transcription factor MYB86 OS=Cucumis melo OX=3656 GN=LOC103490908 PE=4 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 1.3e-192
Identity = 341/379 (89.97%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60
           ME  AA E+GA+  E   GGV+ CDAVASVADCGSGD+ALPMVGEGE TGGRGGKDRVKG
Sbjct: 1   MEGTAAPEIGAAAPE--VGGVDACDAVASVADCGSGDDALPMVGEGEATGGRGGKDRVKG 60

Query: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120
           PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR
Sbjct: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120

Query: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180
           IIVAAHAVHGNKWAAIARLL GRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE
Sbjct: 121 IIVAAHAVHGNKWAAIARLLPGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180

Query: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240
           KTKGSSEETLSCGDVNSFKS +GKD  SREH+DDQ+EDKVPI  EGQF+HEV EQPTL+R
Sbjct: 181 KTKGSSEETLSCGDVNSFKSMDGKDTCSREHLDDQYEDKVPIFVEGQFSHEVNEQPTLFR 240

Query: 241 PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC 300
           PVARVSAFSVYNPLD Q SLR F+RPVPMQGPLIQ SKP+VEASK LEGVYGDRSVPHQC
Sbjct: 241 PVARVSAFSVYNPLDGQGSLRPFLRPVPMQGPLIQVSKPDVEASKFLEGVYGDRSVPHQC 300

Query: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 360
           GHGCC+SHNQGSP++SLLGPEFVDFSEPP SFPSFELAAIATDISNLAWLKSGLENGSVR
Sbjct: 301 GHGCCKSHNQGSPIESLLGPEFVDFSEPPPSFPSFELAAIATDISNLAWLKSGLENGSVR 360

Query: 361 AMGDSAGRLNGSQMQMGRL 380
           AMGDSAGRLNGSQMQMG L
Sbjct: 361 AMGDSAGRLNGSQMQMGHL 377

BLAST of CmaCh03G003820 vs. NCBI nr
Match: XP_022978206.1 (transcription factor MYB1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 754.2 bits (1946), Expect = 5.4e-214
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60
           MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG
Sbjct: 1   MEDAAATEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKG 60

Query: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120
           PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR
Sbjct: 61  PWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDR 120

Query: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180
           IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE
Sbjct: 121 IIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLE 180

Query: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240
           KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR
Sbjct: 181 KTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYR 240

Query: 241 PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC 300
           PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC
Sbjct: 241 PVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQC 300

Query: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 360
           GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR
Sbjct: 301 GHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 360

Query: 361 AMGDSAGRLNGSQMQMGRL 380
           AMGDSAGRLNGSQMQMGRL
Sbjct: 361 AMGDSAGRLNGSQMQMGRL 379

BLAST of CmaCh03G003820 vs. NCBI nr
Match: XP_023543235.1 (transcription factor MYB1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 745.0 bits (1922), Expect = 3.3e-211
Identity = 377/384 (98.18%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 1   MEDAAATEVGASTAE-----GGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGK 60
           MEDAAATEVGAS AE     GGGGGVNECDAVASVADCGSGDEA+PMVGEGETTGGRGGK
Sbjct: 1   MEDAAATEVGASAAEGGGGGGGGGGVNECDAVASVADCGSGDEAVPMVGEGETTGGRGGK 60

Query: 61  DRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFT 120
           DRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFT
Sbjct: 61  DRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFT 120

Query: 121 DEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVD 180
           DEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVD
Sbjct: 121 DEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVD 180

Query: 181 DASLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQ 240
           DASLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQ
Sbjct: 181 DASLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQ 240

Query: 241 PTLYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRS 300
           PTLYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRS
Sbjct: 241 PTLYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRS 300

Query: 301 VPHQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLE 360
           VPHQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLE
Sbjct: 301 VPHQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLE 360

Query: 361 NGSVRAMGDSAGRLNGSQMQMGRL 380
           NGSVRAMGDSAGRLNGSQMQMGRL
Sbjct: 361 NGSVRAMGDSAGRLNGSQMQMGRL 384

BLAST of CmaCh03G003820 vs. NCBI nr
Match: XP_022950705.1 (transcription factor MYB1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 740.3 bits (1910), Expect = 8.1e-210
Identity = 374/382 (97.91%), Postives = 376/382 (98.43%), Query Frame = 0

Query: 1   MEDAAATEVGASTAE---GGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDR 60
           MEDAAATEVGAS AE   GGGGGVNECDAVASVADCGSGDEA+PMVGEGETTGGRGGKDR
Sbjct: 1   MEDAAATEVGASAAEEGGGGGGGVNECDAVASVADCGSGDEAVPMVGEGETTGGRGGKDR 60

Query: 61  VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120
           VKGPWSPEEDAILSRLV KFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE
Sbjct: 61  VKGPWSPEEDAILSRLVGKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120

Query: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180
           EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA
Sbjct: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180

Query: 181 SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPT 240
           SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQ T
Sbjct: 181 SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQST 240

Query: 241 LYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300
           +YRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP
Sbjct: 241 IYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300

Query: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360
           HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG
Sbjct: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360

Query: 361 SVRAMGDSAGRLNGSQMQMGRL 380
           SVRAMGDSAGRLNGSQMQMGRL
Sbjct: 361 SVRAMGDSAGRLNGSQMQMGRL 382

BLAST of CmaCh03G003820 vs. NCBI nr
Match: KAG7033801.1 (Transcription factor MYB1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 739.2 bits (1907), Expect = 1.8e-209
Identity = 374/382 (97.91%), Postives = 376/382 (98.43%), Query Frame = 0

Query: 1   MEDAAATEVGASTAE---GGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDR 60
           MEDAAATEVGAS AE   GGGGGVNECDAVASVADCGSGDEA+PMVGEGETTGGRGGKDR
Sbjct: 1   MEDAAATEVGASAAEEGGGGGGGVNECDAVASVADCGSGDEAVPMVGEGETTGGRGGKDR 60

Query: 61  VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120
           VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE
Sbjct: 61  VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120

Query: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180
           EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA
Sbjct: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180

Query: 181 SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPT 240
           SLEKTKGSSEETLSCGDVNSFKSFEGKDASS EHMDDQFEDKVPIASEGQFTHEVKEQ T
Sbjct: 181 SLEKTKGSSEETLSCGDVNSFKSFEGKDASSPEHMDDQFEDKVPIASEGQFTHEVKEQST 240

Query: 241 LYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300
           +YRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP
Sbjct: 241 IYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300

Query: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360
           HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG
Sbjct: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360

Query: 361 SVRAMGDSAGRLNGSQMQMGRL 380
           SVRAMGDSAGRLNGSQMQMGRL
Sbjct: 361 SVRAMGDSAGRLNGSQMQMGRL 382

BLAST of CmaCh03G003820 vs. NCBI nr
Match: KAG6603611.1 (Transcription factor MYB1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 735.3 bits (1897), Expect = 2.6e-208
Identity = 372/382 (97.38%), Postives = 375/382 (98.17%), Query Frame = 0

Query: 1   MEDAAATEVGASTAE---GGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDR 60
           MEDAAATEVGAS AE   GGGGGVNECDAVASVADCGSGDEA+PMVGEGETTGGRGGKDR
Sbjct: 1   MEDAAATEVGASAAEEGGGGGGGVNECDAVASVADCGSGDEAVPMVGEGETTGGRGGKDR 60

Query: 61  VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120
           VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE
Sbjct: 61  VKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDE 120

Query: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180
           EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA
Sbjct: 121 EDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDA 180

Query: 181 SLEKTKGSSEETLSCGDVNSFKSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPT 240
           SLEKTKGSSEET SCGDVNSF+SFEGKDASS EHMDDQFEDKVPIASEGQFTHEVKEQ T
Sbjct: 181 SLEKTKGSSEETPSCGDVNSFRSFEGKDASSPEHMDDQFEDKVPIASEGQFTHEVKEQST 240

Query: 241 LYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300
           +YRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP
Sbjct: 241 IYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 300

Query: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360
           HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG
Sbjct: 301 HQCGHGCCQSHNQGSPLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENG 360

Query: 361 SVRAMGDSAGRLNGSQMQMGRL 380
           SVRAMGDSAGRLNGSQMQMGRL
Sbjct: 361 SVRAMGDSAGRLNGSQMQMGRL 382

BLAST of CmaCh03G003820 vs. TAIR 10
Match: AT3G09230.1 (myb domain protein 1 )

HSP 1 Score: 312.0 bits (798), Expect = 6.6e-85
Identity = 186/373 (49.87%), Postives = 239/373 (64.08%), Query Frame = 0

Query: 7   TEVGASTAEGGGGGVNECDAVASVADCGSGDEALPMVGEGETTGGRGGKDRVKGPWSPEE 66
           + +G    +G GG V              G++A   VG    T GRG +DRVKGPWS EE
Sbjct: 21  SSIGRGDCDGDGGDV--------------GEDAAGFVG----TSGRGRRDRVKGPWSKEE 80

Query: 67  DAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDRIIVAAH 126
           D +LS LV + GARNWS IAR I GRSGKSCRLRWCNQL+P++ R  FT+ ED+ I+AAH
Sbjct: 81  DDVLSELVKRLGARNWSFIARSIPGRSGKSCRLRWCNQLNPNLIRNSFTEVEDQAIIAAH 140

Query: 127 AVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCTELERIK-LESGN-VVDDASLEKTK- 186
           A+HGNKWA IA+LL GRTDNAIKNHWNS LRRR  + E+ K + +G+ VVDD+  ++T  
Sbjct: 141 AIHGNKWAVIAKLLPGRTDNAIKNHWNSALRRRFIDFEKAKNIGTGSLVVDDSGFDRTTT 200

Query: 187 -GSSEETLS----CGDVNSFKSFEGKDA-SSREHMDDQFEDKVPIASEGQFTHEVKEQPT 246
             SSEETLS    C       S EGK+A +S E  ++Q  +K     EG    + K+ PT
Sbjct: 201 VASSEETLSSGGGCHVTTPIVSPEGKEATTSMEMSEEQCVEKT--NGEGISRQDDKDPPT 260

Query: 247 LYRPVARVSAFSVYNPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVP 306
           L+RPV R+S+F+  N ++   S      P       +Q+SK +    +LLEG Y +R VP
Sbjct: 261 LFRPVPRLSSFNACNHMEGSPS------PHIQDQNQLQSSKQDAAMLRLLEGAYSERFVP 320

Query: 307 HQCGHGCCQSHNQGS-PLDSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLEN 366
             CG GCC ++  GS   +SLLGPEFVD+ + P +FPS ELAAIAT+I +LAWL+SGLE+
Sbjct: 321 QTCGGGCCSNNPDGSFQQESLLGPEFVDYLDSP-TFPSSELAAIATEIGSLAWLRSGLES 366

Query: 367 GSVRAMGDSAGRL 370
            SVR M D+ GRL
Sbjct: 381 SSVRVMEDAVGRL 366

BLAST of CmaCh03G003820 vs. TAIR 10
Match: AT3G55730.1 (myb domain protein 109 )

HSP 1 Score: 300.4 bits (768), Expect = 2.0e-81
Identity = 170/344 (49.42%), Postives = 221/344 (64.24%), Query Frame = 0

Query: 28  ASVADCGSGDEALPMVGEGETTGGRGG-KDRVKGPWSPEEDAILSRLVSKFGARNWSLIA 87
           A +A+  +GD +    G G   GG GG + +VKGPWS EEDA+L++LV K G RNWSLIA
Sbjct: 28  AELAELAAGDSS----GGGGCGGGGGGIRSKVKGPWSTEEDAVLTKLVRKLGPRNWSLIA 87

Query: 88  RGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDRIIVAAHAVHGNKWAAIARLLRGRTDN 147
           RGI GRSGKSCRLRWCNQLDP +KRKPF+DEEDR+I++AHAVHGNKWA IA+LL GRTDN
Sbjct: 88  RGIPGRSGKSCRLRWCNQLDPCLKRKPFSDEEDRMIISAHAVHGNKWAVIAKLLTGRTDN 147

Query: 148 AIKNHWNSTLRRRCTELERIKLESGNVVDDASLEK-------TKGSSEETLSCGDVNSF- 207
           AIKNHWNSTLRR+  +L        N V  AS++           SS++ L  GD+NS  
Sbjct: 148 AIKNHWNSTLRRKYADLWNNGQWMANSVTTASVKNENVDETTNPPSSKQQLPQGDINSSP 207

Query: 208 -KSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPTLYRPVARVSAFSVYNPLDDQ 267
            K  +  D    E  ++  E +            V  +  ++RPVARV AFS+YNP   +
Sbjct: 208 PKPPQVSDVVMEEAANEPQEPQEQQEQAPPVVSNVPTENNVFRPVARVGAFSIYNPTSQK 267

Query: 268 ASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQCGHGCCQSHNQGS-PLDS 327
              R +   VP +GPLIQA+KP+  A K L+ +  +  +P +CGHGC     +     +S
Sbjct: 268 NGYRDY-NIVPCEGPLIQAAKPDSLAGKFLQSLCDEPQIPSKCGHGCSTLPAETKFSRNS 327

Query: 328 LLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLENGSVR 361
           +LGPEFVD+ EP   F + EL +IATD++N+AW+KSGL+N  VR
Sbjct: 328 VLGPEFVDYEEPSAVF-NQELISIATDLNNIAWIKSGLDNAVVR 365

BLAST of CmaCh03G003820 vs. TAIR 10
Match: AT2G39880.1 (myb domain protein 25 )

HSP 1 Score: 250.4 bits (638), Expect = 2.4e-66
Identity = 149/346 (43.06%), Postives = 204/346 (58.96%), Query Frame = 0

Query: 25  DAVASVADCGSGDEALPMVGEGE------TTGGRGGKDRVKGPWSPEEDAILSRLVSKFG 84
           + ++S   C S + A+    E E      +    GGK +VKGPW PE+D  L+RLV   G
Sbjct: 10  ELISSRNPCKSFENAIHKAVEAELAELAKSDANGGGKSKVKGPWLPEQDEALTRLVKMCG 69

Query: 85  ARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFTDEEDRIIVAAHAVHGNKWAAIAR 144
            RNW+LI+RGI GRSGKSCRLRWCNQLDP +KRKPF+DEE+ +I++A AV GNKW+ IA+
Sbjct: 70  PRNWNLISRGIPGRSGKSCRLRWCNQLDPILKRKPFSDEEEHMIMSAQAVLGNKWSVIAK 129

Query: 145 LLRGRTDNAIKNHWNSTLRRRCTELERIKLESGNVVDDASLEKTKGSSEETLSCGDVNSF 204
           LL GRTDNAIKNHWNS LRR+  E  +I L   N      + +   S    +S       
Sbjct: 130 LLPGRTDNAIKNHWNSNLRRKPAEQWKIPLLMSNT---EIVYQLYPSMVRRIS------- 189

Query: 205 KSFEGKDASSREHMDDQFEDKVPIASEGQFTHEVKEQPT-------LYRPVARVSAFSVY 264
                 +AS +EH+    E++  + S+ +   E KE P        +YRPVAR+ AFSV 
Sbjct: 190 ------NASPKEHLPQ--EEETGVLSDDKMDDEAKEPPREQNSKTGVYRPVARMGAFSVC 249

Query: 265 NPLDDQASLRAFIRPVPMQGPLIQASKPEVEASKLLEGVYGDRSVPHQCGHGCCQSHNQG 324
            P         ++   P +GPL+QAS+P+  A K L+ +  D  +P +CGHGCC +H   
Sbjct: 250 KP--------GYM--APCEGPLVQASRPDSLAGKFLQSLCYDPIIPSKCGHGCC-NHQDS 309

Query: 325 SPL--DSLLGPEFVDFSEPPLSFPSFELAAIATDISNLAWLKSGLE 356
           + L   S+LG EFVD+ E   +    EL +I+ D++N AW++SG E
Sbjct: 310 TTLSSSSVLGSEFVDYEEHSSAELDKELISISNDLNNTAWIRSGKE 326

BLAST of CmaCh03G003820 vs. TAIR 10
Match: AT4G37260.1 (myb domain protein 73 )

HSP 1 Score: 169.9 bits (429), Expect = 4.0e-42
Identity = 75/110 (68.18%), Postives = 88/110 (80.00%), Query Frame = 0

Query: 52  RGGKDRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKR 111
           R   +R+KGPWSPEED +L RLV K G RNWSLI++ I GRSGKSCRLRWCNQL P V+ 
Sbjct: 6   RKNMERIKGPWSPEEDDLLQRLVQKHGPRNWSLISKSIPGRSGKSCRLRWCNQLSPEVEH 65

Query: 112 KPFTDEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCT 162
           + F+ EED  I+ AHA  GNKWA I+RLL GRTDNAIKNHWNSTL+R+C+
Sbjct: 66  RAFSQEEDETIIRAHARFGNKWATISRLLNGRTDNAIKNHWNSTLKRKCS 115

BLAST of CmaCh03G003820 vs. TAIR 10
Match: AT2G23290.1 (myb domain protein 70 )

HSP 1 Score: 169.5 bits (428), Expect = 5.3e-42
Identity = 76/106 (71.70%), Postives = 86/106 (81.13%), Query Frame = 0

Query: 56  DRVKGPWSPEEDAILSRLVSKFGARNWSLIARGIAGRSGKSCRLRWCNQLDPSVKRKPFT 115
           DR+KGPWSPEED +L  LV K G RNWSLI++ I GRSGKSCRLRWCNQL P V+ + FT
Sbjct: 10  DRIKGPWSPEEDDLLQSLVQKHGPRNWSLISKSIPGRSGKSCRLRWCNQLSPEVEHRGFT 69

Query: 116 DEEDRIIVAAHAVHGNKWAAIARLLRGRTDNAIKNHWNSTLRRRCT 162
            EED  I+ AHA  GNKWA IARLL GRTDNAIKNHWNSTL+R+C+
Sbjct: 70  AEEDDTIILAHARFGNKWATIARLLNGRTDNAIKNHWNSTLKRKCS 115

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q425759.3e-8449.87Transcription factor MYB1 OS=Arabidopsis thaliana OX=3702 GN=MYB1 PE=2 SV=1[more]
O041923.3e-6543.06Transcription factor MYB25 OS=Arabidopsis thaliana OX=3702 GN=MYB25 PE=2 SV=1[more]
O231605.7e-4168.18Transcription factor MYB73 OS=Arabidopsis thaliana OX=3702 GN=MYB73 PE=1 SV=1[more]
Q9FDW17.4e-4170.48Transcription factor MYB44 OS=Arabidopsis thaliana OX=3702 GN=MYB44 PE=1 SV=1[more]
Q9SN124.8e-4069.81Transcription factor MYB77 OS=Arabidopsis thaliana OX=3702 GN=MYB77 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IM362.6e-214100.00transcription factor MYB1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1GGH83.9e-21097.91transcription factor MYB1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IKI51.0e-19493.14transcription factor MYB1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1DDF11.9e-19389.97transcription factor MYB1 OS=Momordica charantia OX=3673 GN=LOC111019477 PE=4 SV... [more]
A0A1S3BKQ61.3e-19289.97transcription factor MYB86 OS=Cucumis melo OX=3656 GN=LOC103490908 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022978206.15.4e-214100.00transcription factor MYB1-like isoform X1 [Cucurbita maxima][more]
XP_023543235.13.3e-21198.18transcription factor MYB1-like [Cucurbita pepo subsp. pepo][more]
XP_022950705.18.1e-21097.91transcription factor MYB1-like isoform X1 [Cucurbita moschata][more]
KAG7033801.11.8e-20997.91Transcription factor MYB1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6603611.12.6e-20897.38Transcription factor MYB1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT3G09230.16.6e-8549.87myb domain protein 1 [more]
AT3G55730.12.0e-8149.42myb domain protein 109 [more]
AT2G39880.12.4e-6643.06myb domain protein 25 [more]
AT4G37260.14.0e-4268.18myb domain protein 73 [more]
AT2G23290.15.3e-4271.70myb domain protein 70 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001005SANT/Myb domainSMARTSM00717santcoord: 58..107
e-value: 3.2E-15
score: 66.5
coord: 110..158
e-value: 4.3E-16
score: 69.5
IPR001005SANT/Myb domainPROSITEPS50090MYB_LIKEcoord: 106..156
score: 9.709374
IPR001005SANT/Myb domainPROSITEPS50090MYB_LIKEcoord: 54..105
score: 11.439622
IPR001005SANT/Myb domainCDDcd00167SANTcoord: 113..156
e-value: 1.3088E-12
score: 59.8966
IPR001005SANT/Myb domainCDDcd00167SANTcoord: 61..103
e-value: 6.27089E-15
score: 66.445
NoneNo IPR availablePFAMPF13921Myb_DNA-bind_6coord: 62..119
e-value: 4.9E-16
score: 58.7
NoneNo IPR availableGENE3D1.10.10.60coord: 113..159
e-value: 1.2E-19
score: 71.9
NoneNo IPR availableGENE3D1.10.10.60coord: 58..110
e-value: 7.9E-22
score: 79.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..62
NoneNo IPR availablePANTHERPTHR45614MYB PROTEIN-RELATEDcoord: 27..357
NoneNo IPR availablePANTHERPTHR45614:SF155SUBFAMILY NOT NAMEDcoord: 27..357
IPR017930Myb domainPROSITEPS51294HTH_MYBcoord: 54..109
score: 26.784349
IPR017930Myb domainPROSITEPS51294HTH_MYBcoord: 110..160
score: 23.793028
IPR009057Homeobox-like domain superfamilySUPERFAMILY46689Homeodomain-likecoord: 56..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G003820.1CmaCh03G003820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding