CmaCh03G003390 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G003390
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPyruvate decarboxylase
LocationCma_Chr03: 4102673 .. 4105462 (-)
RNA-Seq ExpressionCmaCh03G003390
SyntenyCmaCh03G003390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAATGGCGGCGGTTCGCTCTATGCTCAAGCCTCCGACTTCCAATGGTTCCTATAAACCCCAATCAAAACTTCAAACTAATAATTGTAACAATGGAGTTGAAATAGTCGAGTCCATGGTTACACCGTCTACCTTGGGCCACTACCTAGCCCGACGCCTCGTTCAAATTGGCGTCTCGGATATCTTCTCGGTTCCAGGCGACTCCAATCTCGTGCTGCTCGATTACTTCGTGGCAGAAAAAGGCCTAAACCTTGTGGGGTGTTGTAGCGAGCTCAATGCTGGGTATGCCGCTGATGGCTACGCCAGACGCCGCGGCGTTGGTTCTTGTGTTGTCACCTTCACAGTGGGTGGGCTCAGCCTGATCAACGCCATTGCCGGTGCTTACTCGGAGGATCTTCCTCTGGTTTGCATCGTGGGTGCACCAAACTCGAACGAATATGGGAGTAACAAGATTCTCCACCATACCATTGGCCTACCGGATTTCAGTCAAGAGCTCCGATGCTTTCAAAATGTCACCTGTTATCAGGTAGTCTTACTGAAATGGTGTGTAAATTTTTTATGGGTCTCTGTTTTTTAGCTCTGTTTTGCTGTAATTTTTTATGGGTCTCTGTTTTTTAGCTCTGTTTTGCTGTAAATTTTCATGGGTCTCTGTTTTTGAGCTCTGTTTTGCTGTTAATTTTTTATGGGTCTCTGTTTTTTAGCTCTGTTTTGCTGTTCTTGTGGCTGTAGGCAGTGATCAACAATTTGGAAGACGCACAATGGGAGATCGATACTGCAATTTCGAAGTGTTTGGAACAGAGTAAGCCCGTTTATATCAGCATTTCTTGCAATTTGGCGGCCATTCCTCATCCTTCTTTCTCTATGAAACAATTCCTTCCACTCTCCATCTCTTCCAAGCAAACTAACCAGATGGGTTTGGAGATGGCCGTGGAGATGGCAGCTCAGATTCTAAACACCGCCGTTAAACCCATCATGGTCGGCGGAAAGAAGCTGCGAGTAGCTAAAGCACAAGCGGCGTTCGTGGAGCTAGCCGATGTTTGTGACTACGCAGTCGCCGTTACGCCATCGGCTAAAGGCATGTTCCCGGAGAACCACCGTCATTTCATCGGAACTTACTGGGGATCTCTCAGCTCAGCCTTCTGCGGCGAGACAGTGGAAATCGCCGACGCTTCAATCTTCGCCGGAGCTATTTTCGACGAACTGGAAACCGTCGGCTACTCGCTTTCGTACAAGAAGAATAAGGCGATTATTGTAGAATCGGACCGCGTTGTGTTTCCAAATGGGCCGATTTTTGGGCCGATTTTAATGAAGGATTTTCTTCGGGCCCTTGCTAAGCGGCTCAAGCCCAATCCAACAGCTTACGAGAATTATACTCGGATTTATATTCCAAAGTCGGGTCCTACCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAATGTCCTTTTCAAACATATACAAAAAATGCTGTCGGGAAACATGGCGGTAATTTCTGAAGCTGGTGATTCATGGTTTCACTGTCAAAAACTAGAATTACCACGATCATGCGGGTAAAATTCTTCCCAAACTTTAAACTCAATGGGTTAAATATTATGAGTACGATAATGGAATAATCTTCGGAGAAATATAGGTTCGAGATGCAGCTGGTTTATGCCTCCATCGGGTGGTCTCTCGGCGCCACGCTCGGCTACGCTCAAGCTTCGTCGGATGATCGTGTGGTTCTTTGCATTGGCGATGGGAGCTTCCAGGTAAATTTTCATTCTATTTTTGTTGTATGTTATTATCCACTTTGAACATAAACTCTCGTGATTTTATTCTTAGTGTTTCCAAAATGCTTCGTAACAATAAAGATAGTATTCCTTATAAACCTATAATCAACCCCTTAATTGACGGACGTAGGACTCCTCTCCCAACAATTCTATCATTGAACAAAGTACACTATAGAGCCCCCTTAGGCGTATGGAGCTCTCGAACAGTCTCTCCTTAATCGAGGCTCGATTCTTTCTCTAGAGCCCTCAAACAAAATACACAATTTGTTCGATACTTGAGTCACTTTTGATTACAAAACTGTTGAGTATTCTATTGACATGACTAAATTAAGGACATGACTTTAATACCATGTTAGAAACCACAACTCTCCACAATGGTATAATATTGTTCACTTTCACCATAAACTCTCATAATATTCTTTACTTATGATTAACTGATGTAGAACTCCTCTCCCAACCATCCTCAAAATACCATCAAAATTTTACCTCATTTTTTCACCGTTTTTGTTTAAAATAAACAGATGGCTCCACAAGATTTGTCAACGATGCTACGATTAGGACAGAGAAACATAATATTTCTAATAAACAACGGTGGATACACAATTGAAGTCGAGATTCACGACGGCCCGTACAATGTCATCAAGAACTGGGACTACACGGGTCTAGTCGAGGCCATGCACAATCAAAACGGTAACTGTTGGACGACCAAGGTATGTTTTTTTTTTTAAATAAAAATTAAAAACTAACTATAGTTAATTGAGATGCATAAAGTAGTTAAGAAGAAGAAGGTTTGAATATTAACGAACAGGTTGAAACGGAGGAAGAGTTGGTGAAAGCGATTGAAATGGCAACGGGAGATAAAAACGAGTGCTTGTGCTTCATCGAGGCCATTGTTCATCGAGATGATACCAGTAAAGAGTTGCTTGACTTTGGGAACAAATTGGCTGCTACCAACAGTCGTCCTCCCAAATAATATTTACATCACTTAATCAACTTTTATATCTCAATAATGTGTTCTTTCT

mRNA sequence

ATGCCAATGGCGGCGGTTCGCTCTATGCTCAAGCCTCCGACTTCCAATGGTTCCTATAAACCCCAATCAAAACTTCAAACTAATAATTGTAACAATGGAGTTGAAATAGTCGAGTCCATGGTTACACCGTCTACCTTGGGCCACTACCTAGCCCGACGCCTCGTTCAAATTGGCGTCTCGGATATCTTCTCGGTTCCAGGCGACTCCAATCTCGTGCTGCTCGATTACTTCGTGGCAGAAAAAGGCCTAAACCTTGTGGGGTGTTGTAGCGAGCTCAATGCTGGGTATGCCGCTGATGGCTACGCCAGACGCCGCGGCGTTGGTTCTTGTGTTGTCACCTTCACAGTGGGTGGGCTCAGCCTGATCAACGCCATTGCCGGTGCTTACTCGGAGGATCTTCCTCTGGTTTGCATCGTGGGTGCACCAAACTCGAACGAATATGGGAGTAACAAGATTCTCCACCATACCATTGGCCTACCGGATTTCAGTCAAGAGCTCCGATGCTTTCAAAATGTCACCTGTTATCAGGCAGTGATCAACAATTTGGAAGACGCACAATGGGAGATCGATACTGCAATTTCGAAGTGTTTGGAACAGAGTAAGCCCGTTTATATCAGCATTTCTTGCAATTTGGCGGCCATTCCTCATCCTTCTTTCTCTATGAAACAATTCCTTCCACTCTCCATCTCTTCCAAGCAAACTAACCAGATGGGTTTGGAGATGGCCGTGGAGATGGCAGCTCAGATTCTAAACACCGCCGTTAAACCCATCATGGTCGGCGGAAAGAAGCTGCGAGTAGCTAAAGCACAAGCGGCGTTCGTGGAGCTAGCCGATGTTTGTGACTACGCAGTCGCCGTTACGCCATCGGCTAAAGGCATGTTCCCGGAGAACCACCGTCATTTCATCGGAACTTACTGGGGATCTCTCAGCTCAGCCTTCTGCGGCGAGACAGTGGAAATCGCCGACGCTTCAATCTTCGCCGGAGCTATTTTCGACGAACTGGAAACCGTCGGCTACTCGCTTTCGTACAAGAAGAATAAGGCGATTATTGTAGAATCGGACCGCGTTGTGTTTCCAAATGGGCCGATTTTTGGGCCGATTTTAATGAAGGATTTTCTTCGGGCCCTTGCTAAGCGGCTCAAGCCCAATCCAACAGCTTACGAGAATTATACTCGGATTTATATTCCAAAGTCGGGTCCTACCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAATGTCCTTTTCAAACATATACAAAAAATGCTGTCGGGAAACATGGCGGTAATTTCTGAAGCTGGTGATTCATGGTTTCACTGTCAAAAACTAGAATTACCACGATCATGCGGGTTCGAGATGCAGCTGGTTTATGCCTCCATCGGGTGGTCTCTCGGCGCCACGCTCGGCTACGCTCAAGCTTCGTCGGATGATCGTGTGGTTCTTTGCATTGGCGATGGGAGCTTCCAGATGGCTCCACAAGATTTGTCAACGATGCTACGATTAGGACAGAGAAACATAATATTTCTAATAAACAACGGTGGATACACAATTGAAGTCGAGATTCACGACGGCCCGTACAATGTCATCAAGAACTGGGACTACACGGGTCTAGTCGAGGCCATGCACAATCAAAACGGTAACTGTTGGACGACCAAGGTTGAAACGGAGGAAGAGTTGGTGAAAGCGATTGAAATGGCAACGGGAGATAAAAACGAGTGCTTGTGCTTCATCGAGGCCATTGTTCATCGAGATGATACCAGTAAAGAGTTGCTTGACTTTGGGAACAAATTGGCTGCTACCAACAGTCGTCCTCCCAAATAATATTTACATCACTTAATCAACTTTTATATCTCAATAATGTGTTCTTTCT

Coding sequence (CDS)

ATGCCAATGGCGGCGGTTCGCTCTATGCTCAAGCCTCCGACTTCCAATGGTTCCTATAAACCCCAATCAAAACTTCAAACTAATAATTGTAACAATGGAGTTGAAATAGTCGAGTCCATGGTTACACCGTCTACCTTGGGCCACTACCTAGCCCGACGCCTCGTTCAAATTGGCGTCTCGGATATCTTCTCGGTTCCAGGCGACTCCAATCTCGTGCTGCTCGATTACTTCGTGGCAGAAAAAGGCCTAAACCTTGTGGGGTGTTGTAGCGAGCTCAATGCTGGGTATGCCGCTGATGGCTACGCCAGACGCCGCGGCGTTGGTTCTTGTGTTGTCACCTTCACAGTGGGTGGGCTCAGCCTGATCAACGCCATTGCCGGTGCTTACTCGGAGGATCTTCCTCTGGTTTGCATCGTGGGTGCACCAAACTCGAACGAATATGGGAGTAACAAGATTCTCCACCATACCATTGGCCTACCGGATTTCAGTCAAGAGCTCCGATGCTTTCAAAATGTCACCTGTTATCAGGCAGTGATCAACAATTTGGAAGACGCACAATGGGAGATCGATACTGCAATTTCGAAGTGTTTGGAACAGAGTAAGCCCGTTTATATCAGCATTTCTTGCAATTTGGCGGCCATTCCTCATCCTTCTTTCTCTATGAAACAATTCCTTCCACTCTCCATCTCTTCCAAGCAAACTAACCAGATGGGTTTGGAGATGGCCGTGGAGATGGCAGCTCAGATTCTAAACACCGCCGTTAAACCCATCATGGTCGGCGGAAAGAAGCTGCGAGTAGCTAAAGCACAAGCGGCGTTCGTGGAGCTAGCCGATGTTTGTGACTACGCAGTCGCCGTTACGCCATCGGCTAAAGGCATGTTCCCGGAGAACCACCGTCATTTCATCGGAACTTACTGGGGATCTCTCAGCTCAGCCTTCTGCGGCGAGACAGTGGAAATCGCCGACGCTTCAATCTTCGCCGGAGCTATTTTCGACGAACTGGAAACCGTCGGCTACTCGCTTTCGTACAAGAAGAATAAGGCGATTATTGTAGAATCGGACCGCGTTGTGTTTCCAAATGGGCCGATTTTTGGGCCGATTTTAATGAAGGATTTTCTTCGGGCCCTTGCTAAGCGGCTCAAGCCCAATCCAACAGCTTACGAGAATTATACTCGGATTTATATTCCAAAGTCGGGTCCTACCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAATGTCCTTTTCAAACATATACAAAAAATGCTGTCGGGAAACATGGCGGTAATTTCTGAAGCTGGTGATTCATGGTTTCACTGTCAAAAACTAGAATTACCACGATCATGCGGGTTCGAGATGCAGCTGGTTTATGCCTCCATCGGGTGGTCTCTCGGCGCCACGCTCGGCTACGCTCAAGCTTCGTCGGATGATCGTGTGGTTCTTTGCATTGGCGATGGGAGCTTCCAGATGGCTCCACAAGATTTGTCAACGATGCTACGATTAGGACAGAGAAACATAATATTTCTAATAAACAACGGTGGATACACAATTGAAGTCGAGATTCACGACGGCCCGTACAATGTCATCAAGAACTGGGACTACACGGGTCTAGTCGAGGCCATGCACAATCAAAACGGTAACTGTTGGACGACCAAGGTTGAAACGGAGGAAGAGTTGGTGAAAGCGATTGAAATGGCAACGGGAGATAAAAACGAGTGCTTGTGCTTCATCGAGGCCATTGTTCATCGAGATGATACCAGTAAAGAGTTGCTTGACTTTGGGAACAAATTGGCTGCTACCAACAGTCGTCCTCCCAAATAA

Protein sequence

MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPPK
Homology
BLAST of CmaCh03G003390 vs. ExPASy Swiss-Prot
Match: A2Y5L9 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 3.2e-235
Identity = 391/594 (65.82%), Postives = 475/594 (79.97%), Query Frame = 0

Query: 15  SNGSYKPQ----SKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSN 74
           SNG  KP       L   + N  +    +    +TLG +LARRLVQIG +D+F+VPGD N
Sbjct: 10  SNGVAKPSCNSVGSLPVVSSNAVIHPPVTSAAGATLGRHLARRLVQIGATDVFAVPGDFN 69

Query: 75  LVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYS 134
           L LLDY +AE GL L+GCC+ELNAGYAADGYAR RGVG+C VTFTVGGLS++NAIAGAYS
Sbjct: 70  LTLLDYLIAEPGLKLIGCCNELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIAGAYS 129

Query: 135 EDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEID 194
           E+LP++CIVG PNSN+YG+N+ILHHTIGLPDFSQELRCFQ +TCYQAVINNL+DA  +ID
Sbjct: 130 ENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTITCYQAVINNLDDAHEQID 189

Query: 195 TAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQIL 254
           TAI+  L +SKPVYIS+ CNLA + HP+FS ++ +PL IS + +N+  LE AVE AA  L
Sbjct: 190 TAIATALRESKPVYISVGCNLAGLSHPTFS-REPVPLFISPRLSNKANLEYAVEAAADFL 249

Query: 255 NTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLS 314
           N AVKP+MVGG K+RVAKA+ AF  +A+   Y +AV PSAKG+ PE+H  FIGTYWG++S
Sbjct: 250 NKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPIAVMPSAKGLVPEHHPRFIGTYWGAVS 309

Query: 315 SAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMK 374
           + FC E VE ADA +FAG IF++  +VGYSL  K+ KA+IV+ DRVV  NGP FG ILM 
Sbjct: 310 TTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNGPAFGCILMT 369

Query: 375 DFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVIS 434
           +FL ALAKRL  N TAY+NY RI+IP   P   +P E LRVN+LFKHI++MLSG+ AVI+
Sbjct: 370 EFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIA 429

Query: 435 EAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMA 494
           E GDSWF+CQKL LP  CG+E Q+ Y SIGWS+GATLGYAQA+ D RV+ CIGDGSFQM 
Sbjct: 430 ETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMT 489

Query: 495 PQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTT 554
            QD+STMLR GQ++IIFLINNGGYTIEVEIHDGPYNVIKNWDYTGL++A+HN +GNCWT 
Sbjct: 490 AQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIHNSDGNCWTK 549

Query: 555 KVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPP 605
           KV TEEEL++AI  ATG K +CLCFIE IVH+DDTSKELL++G++++A NSRPP
Sbjct: 550 KVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPP 602

BLAST of CmaCh03G003390 vs. ExPASy Swiss-Prot
Match: Q0DHF6 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 9.2e-235
Identity = 391/594 (65.82%), Postives = 474/594 (79.80%), Query Frame = 0

Query: 15  SNGSYKPQ----SKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSN 74
           SNG  KP       L   + N  +    +    +TLG +LARRLVQIG +D+F+VPGD N
Sbjct: 10  SNGVAKPSCNSVGSLPVVSSNAVINPPVTSAAGATLGRHLARRLVQIGATDVFAVPGDFN 69

Query: 75  LVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYS 134
           L LLDY +AE GL L+GCC+ELNAGYAADGYAR RGVG+C VTFTVGGLS++NAIAGAYS
Sbjct: 70  LTLLDYLIAEPGLKLIGCCNELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIAGAYS 129

Query: 135 EDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEID 194
           E+LP++CIVG PNSN+YG+N+ILHHTIGLPDFSQELRCFQ +TCYQAVINNL+DA  +ID
Sbjct: 130 ENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTITCYQAVINNLDDAHEQID 189

Query: 195 TAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQIL 254
           TAI+  L +SKPVYIS+ CNLA + HP+FS ++ +PL IS + +N+  LE AVE AA  L
Sbjct: 190 TAIATALRESKPVYISVGCNLAGLSHPTFS-REPVPLFISPRLSNKANLEYAVEAAADFL 249

Query: 255 NTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLS 314
           N AVKP+MVGG K+RVAKA+ AF  +A+   Y  AV PSAKG+ PE+H  FIGTYWG++S
Sbjct: 250 NKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPFAVMPSAKGLVPEHHPRFIGTYWGAVS 309

Query: 315 SAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMK 374
           + FC E VE ADA +FAG IF++  +VGYSL  K+ KA+IV+ DRVV  NGP FG ILM 
Sbjct: 310 TTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNGPAFGCILMT 369

Query: 375 DFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVIS 434
           +FL ALAKRL  N TAY+NY RI+IP   P   +P E LRVN+LFKHI++MLSG+ AVI+
Sbjct: 370 EFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIA 429

Query: 435 EAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMA 494
           E GDSWF+CQKL LP  CG+E Q+ Y SIGWS+GATLGYAQA+ D RV+ CIGDGSFQM 
Sbjct: 430 ETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMT 489

Query: 495 PQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTT 554
            QD+STMLR GQ++IIFLINNGGYTIEVEIHDGPYNVIKNWDYTGL++A+HN +GNCWT 
Sbjct: 490 AQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIHNSDGNCWTK 549

Query: 555 KVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPP 605
           KV TEEEL++AI  ATG K +CLCFIE IVH+DDTSKELL++G++++A NSRPP
Sbjct: 550 KVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPP 602

BLAST of CmaCh03G003390 vs. ExPASy Swiss-Prot
Match: Q9FFT4 (Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 1.7e-233
Identity = 392/583 (67.24%), Postives = 470/583 (80.62%), Query Frame = 0

Query: 31  NNGVEIVE-------SMVTP--STLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEK 90
           N GV  V+       + V+P  +TLG YLARRLV+IGV+D+FSVPGD NL LLD+ +AE 
Sbjct: 23  NGGVSTVQNTSPLHSTTVSPCDATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEP 82

Query: 91  GLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVGA 150
            L L+GCC+ELNAGYAADGYAR RGVG+CVVTFTVGGLS++NAIAGAYSE+LPL+CIVG 
Sbjct: 83  NLKLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGG 142

Query: 151 PNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSK 210
           PNSN+YG+N+ILHHTIGLPDF+QELRCFQ VTC+QAVINNLE+A   IDTAIS  L++SK
Sbjct: 143 PNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESK 202

Query: 211 PVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVGG 270
           PVYISISCNL AIP P+FS +  +P  +  K +NQ+GL+ AVE AA+ LN AVKP++VGG
Sbjct: 203 PVYISISCNLPAIPLPTFS-RHPVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGG 262

Query: 271 KKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIA 330
            K+RVAKA  AFVELAD   Y +AV PSAKG  PE+H+HFIGTYWG++S+AFC E VE A
Sbjct: 263 PKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESA 322

Query: 331 DASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRLK 390
           DA +FAG IF++  +VGYSL  KK KAIIV+ DRV   NGP FG +LMKDFL  LAKR+K
Sbjct: 323 DAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIK 382

Query: 391 PNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQK 450
            N T+YENY RIY+P+  P +  P E+LRVNVLF+HIQ MLS   AV++E GDSWF+CQK
Sbjct: 383 HNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQK 442

Query: 451 LELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRLG 510
           L+LP  CG+E Q+ Y SIGWS+GATLGYAQA  + RV+ CIGDGSFQ+  QD+STM+R G
Sbjct: 443 LKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCG 502

Query: 511 QRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVKA 570
           Q+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YT  VEA+HN  G CWT KV  EEELVKA
Sbjct: 503 QKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKA 562

Query: 571 IEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPP 605
           I  AT ++ E  CFIE IVH+DDTSKELL++G++++A NSRPP
Sbjct: 563 INTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of CmaCh03G003390 vs. ExPASy Swiss-Prot
Match: O82647 (Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.3e-233
Identity = 391/584 (66.95%), Postives = 471/584 (80.65%), Query Frame = 0

Query: 21  PQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAE 80
           P S +  N C+            +TLG +LARRLVQ GV+D+FSVPGD NL LLD+ +AE
Sbjct: 34  PSSAITINYCD------------ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAE 93

Query: 81  KGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVG 140
             LNL+GCC+ELNAGYAADGYAR RGVG+CVVTFTVGGLS++NAIAGAYSE+LPL+CIVG
Sbjct: 94  PDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVG 153

Query: 141 APNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQS 200
            PNSN+YG+N+ILHHTIGLPDFSQELRCFQ VTCYQAV+NNL+DA  +ID AIS  L++S
Sbjct: 154 GPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKES 213

Query: 201 KPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVG 260
           KPVYIS+SCNLAAIPH +FS +  +P S++ + +N+MGLE AVE   + LN AVKP+MVG
Sbjct: 214 KPVYISVSCNLAAIPHHTFS-RDPVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVG 273

Query: 261 GKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEI 320
           G KLRVAKA  AFVELAD   YA+A+ PSAKG  PE+H HFIGTYWG++S+ FC E VE 
Sbjct: 274 GPKLRVAKACDAFVELADASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVES 333

Query: 321 ADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRL 380
           ADA IFAG IF++  +VGYSL  KK KAI+V+ DR+   NGP FG ILM DF R L+KR+
Sbjct: 334 ADAYIFAGPIFNDYSSVGYSLLLKKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRV 393

Query: 381 KPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQ 440
           K N TAYENY RI++P+  P + E  E LRVN +F+HIQKMLS   AVI+E GDSWF+CQ
Sbjct: 394 KRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQ 453

Query: 441 KLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRL 500
           KL+LP+ CG+E Q+ Y SIGWS+GATLGYAQAS + RV+  IGDGSFQ+  QD+STMLR 
Sbjct: 454 KLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRN 513

Query: 501 GQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVK 560
           GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YTGLV+A+HN  GNCWT KV  EEELV+
Sbjct: 514 GQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVE 573

Query: 561 AIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPP 605
           AI  AT +K +CLCFIE I+H+DDTSKELL++G++++A NSRPP
Sbjct: 574 AITTATTEKKDCLCFIEVILHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of CmaCh03G003390 vs. ExPASy Swiss-Prot
Match: P28516 (Pyruvate decarboxylase 1 OS=Zea mays OX=4577 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.3e-233
Identity = 382/561 (68.09%), Postives = 461/561 (82.17%), Query Frame = 0

Query: 44  STLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYAR 103
           +TLG +LARRLVQIG SD+F+VPGD NL LLDY +AE GL LVGCC+ELNAGYAADGYAR
Sbjct: 48  ATLGRHLARRLVQIGASDVFAVPGDFNLTLLDYLIAEPGLTLVGCCNELNAGYAADGYAR 107

Query: 104 RRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFS 163
            RGVG+C VTFTVGGLS++NAIAGAYSE+LP+VCIVG PNSN+YG+N+ILHHTIGLPDFS
Sbjct: 108 SRGVGACAVTFTVGGLSVLNAIAGAYSENLPVVCIVGGPNSNDYGTNRILHHTIGLPDFS 167

Query: 164 QELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQ 223
           QELRCFQ +TCYQA+INNL+DA  +IDTAI+  L +SKPVYIS+SCNLA + HP+FS + 
Sbjct: 168 QELRCFQTITCYQAIINNLDDAHEQIDTAIATALRESKPVYISVSCNLAGLSHPTFS-RD 227

Query: 224 FLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYA 283
            +P+ IS + +N+  LE AVE AA  LN AVKP+MVGG K+RVAKA+ AF  +AD   Y 
Sbjct: 228 PVPMFISPRLSNKANLEYAVEAAADFLNKAVKPVMVGGPKIRVAKAREAFAAVADASGYP 287

Query: 284 VAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSY 343
            AV P+AKG+ PE+H  FIGTYWG++S+ FC E VE ADA +FAG IF++  +VGYSL  
Sbjct: 288 FAVMPAAKGLVPEHHPRFIGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 347

Query: 344 KKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQS 403
           K+ KA+IV+ DR+V  +GP FG ILM +FLRALAKRL+ N TAY+NY RI++P   P   
Sbjct: 348 KREKAVIVQPDRMVVGDGPAFGCILMPEFLRALAKRLRRNTTAYDNYRRIFVPDREPPNG 407

Query: 404 EPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSL 463
           +P E LRVNVLFKHI+ MLSG+ AV++E GDSWF+CQKL LP  CG+E Q+ Y SIGWS+
Sbjct: 408 KPNEPLRVNVLFKHIKGMLSGDSAVVAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSV 467

Query: 464 GATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDG 523
           GATLGYAQA+ D RV+ CIGDGSFQ+  QD+STMLR GQ++IIFLINNGGYTIEVEIHDG
Sbjct: 468 GATLGYAQAAKDKRVIACIGDGSFQVTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDG 527

Query: 524 PYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRD 583
           PYNVIKNWDYTGLV A+HN  GNCWT KV TEE+L +AI   TG K +CLCFIE IVH+D
Sbjct: 528 PYNVIKNWDYTGLVNAIHNSEGNCWTMKVRTEEQLKEAIATVTGAKKDCLCFIEVIVHKD 587

Query: 584 DTSKELLDFGNKLAATNSRPP 605
           DTSKELL++G++++A NSRPP
Sbjct: 588 DTSKELLEWGSRVSAANSRPP 607

BLAST of CmaCh03G003390 vs. ExPASy TrEMBL
Match: A0A6J1IP68 (Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111478194 PE=3 SV=1)

HSP 1 Score: 1222.6 bits (3162), Expect = 0.0e+00
Identity = 605/605 (100.00%), Postives = 605/605 (100.00%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS
Sbjct: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS
Sbjct: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180
           LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN
Sbjct: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180

Query: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 240
           NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE
Sbjct: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 240

Query: 241 MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300
           MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH
Sbjct: 241 MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300

Query: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360
           FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN
Sbjct: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360

Query: 361 GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420
           GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK
Sbjct: 361 GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420

Query: 421 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL 480
           MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL
Sbjct: 421 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL 480

Query: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 540
           CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM
Sbjct: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 540

Query: 541 HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600
           HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN
Sbjct: 541 HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600

Query: 601 SRPPK 606
           SRPPK
Sbjct: 601 SRPPK 605

BLAST of CmaCh03G003390 vs. ExPASy TrEMBL
Match: A0A6J1GDK7 (Pyruvate decarboxylase OS=Cucurbita moschata OX=3662 GN=LOC111453217 PE=3 SV=1)

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 593/605 (98.02%), Postives = 600/605 (99.17%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS
Sbjct: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+CVVTFTVGGLS
Sbjct: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACVVTFTVGGLS 120

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180
           LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN
Sbjct: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180

Query: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 240
           NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQ+NQMGLE
Sbjct: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQSNQMGLE 240

Query: 241 MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300
           MAVEMAAQ+LNTAVKP+MVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH
Sbjct: 241 MAVEMAAQLLNTAVKPVMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300

Query: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360
           FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN
Sbjct: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360

Query: 361 GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420
           GP FGP+LMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK
Sbjct: 361 GPSFGPVLMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420

Query: 421 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL 480
           MLSGNMAVISEAGDSWFHCQ LELPRSCGFEMQLVYASIGWSLGATLGYAQAS +DRVVL
Sbjct: 421 MLSGNMAVISEAGDSWFHCQNLELPRSCGFEMQLVYASIGWSLGATLGYAQASPEDRVVL 480

Query: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 540
           CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVEAM
Sbjct: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWNYTALVEAM 540

Query: 541 HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600
           HNQ GNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN
Sbjct: 541 HNQTGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600

Query: 601 SRPPK 606
           SRPPK
Sbjct: 601 SRPPK 605

BLAST of CmaCh03G003390 vs. ExPASy TrEMBL
Match: A0A5D3CG96 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00020 PE=3 SV=1)

HSP 1 Score: 1058.9 bits (2737), Expect = 7.9e-306
Identity = 519/609 (85.22%), Postives = 559/609 (91.79%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MP  A RSMLKPPT+NG YKP SK QT   NNG ++ +  V PSTLGHYLARRLVQIG+S
Sbjct: 20  MPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSVVPSTLGHYLARRLVQIGIS 79

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+C VTFTVGGLS
Sbjct: 80  DIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLS 139

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYG--SNKILHHTIGLPDFSQELRCFQNVTCYQAV 180
           LINAIAGAYSEDLP+VCIVG PNSNEYG  + KILHHTIGLPDFSQEL+CF+NVTCYQAV
Sbjct: 140 LINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGLPDFSQELQCFRNVTCYQAV 199

Query: 181 INNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMG 240
           INNL+DAQWEID AISKCLE+SKPVYISI+CNLAAIPHPSFSM+Q +PLSIS KQ+NQM 
Sbjct: 200 INNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSFSMQQLIPLSISPKQSNQMS 259

Query: 241 LEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENH 300
           LEMAVE AAQ+LN AVKP++VGGKKLRVAKAQAAFVELA+ CDYAVAVTPSAKGMFPENH
Sbjct: 260 LEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENH 319

Query: 301 RHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVF 360
            HF+GTYWG LS+AFCGETVEIADASIF GAIFDELETVG+SLSYKKNKAIIVE+D VVF
Sbjct: 320 PHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVF 379

Query: 361 PNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHI 420
           PNGP FGPILMKDFL ALAKR+KPN TAYENY RIYIP+S P  SEPGEALRVNVLFKHI
Sbjct: 380 PNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHI 439

Query: 421 QKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQA--SSDD 480
           QKML+ +M VISEAGDSWFH QKL LP+SCG+EMQ+VYASIGWSLGATLGYAQA  SS+D
Sbjct: 440 QKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSND 499

Query: 481 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGL 540
           RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ L
Sbjct: 500 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSAL 559

Query: 541 VEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKL 600
           V+AMHN NGNCWTTKV+TEEELVKAIE+AT D N+CLCFIE IVHRDDTSKELL+FGNKL
Sbjct: 560 VDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKL 619

Query: 601 AATNSRPPK 606
           A  NSRPP+
Sbjct: 620 AEMNSRPPE 628

BLAST of CmaCh03G003390 vs. ExPASy TrEMBL
Match: A0A5A7T4K4 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00340 PE=3 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 2.3e-305
Identity = 518/609 (85.06%), Postives = 559/609 (91.79%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MP  A RSMLKPPT+NG YKP SK QT   NNG ++ +  V PSTLGHYLARRLVQIG+S
Sbjct: 20  MPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSVVPSTLGHYLARRLVQIGIS 79

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+C VTFTVGGLS
Sbjct: 80  DIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLS 139

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYG--SNKILHHTIGLPDFSQELRCFQNVTCYQAV 180
           LINAIAGAYSEDLP+VCIVG PNSNEYG  + KILHHTIGLPDFSQEL+CF+NVTCYQAV
Sbjct: 140 LINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGLPDFSQELQCFRNVTCYQAV 199

Query: 181 INNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMG 240
           INNL+DAQWEID AISKCLE+SKPVYISI+CNLAAIPHPSFS++Q +PLSIS KQ+NQM 
Sbjct: 200 INNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSFSVQQLIPLSISPKQSNQMS 259

Query: 241 LEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENH 300
           LEMAVE AAQ+LN AVKP++VGGKKLRVAKAQAAFVELA+ CDYAVAVTPSAKGMFPENH
Sbjct: 260 LEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENH 319

Query: 301 RHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVF 360
            HF+GTYWG LS+AFCGETVEIADASIF GAIFDELETVG+SLSYKKNKAIIVE+D VVF
Sbjct: 320 PHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVF 379

Query: 361 PNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHI 420
           PNGP FGPILMKDFL ALAKR+KPN TAYENY RIYIP+S P  SEPGEALRVNVLFKHI
Sbjct: 380 PNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHI 439

Query: 421 QKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQA--SSDD 480
           QKML+ +M VISEAGDSWFH QKL LP+SCG+EMQ+VYASIGWSLGATLGYAQA  SS+D
Sbjct: 440 QKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSND 499

Query: 481 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGL 540
           RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ L
Sbjct: 500 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSAL 559

Query: 541 VEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKL 600
           V+AMHN NGNCWTTKV+TEEELVKAIE+AT D N+CLCFIE IVHRDDTSKELL+FGNKL
Sbjct: 560 VDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKL 619

Query: 601 AATNSRPPK 606
           A  NSRPP+
Sbjct: 620 AEMNSRPPE 628

BLAST of CmaCh03G003390 vs. ExPASy TrEMBL
Match: A0A1S3CL30 (Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502191 PE=3 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 2.3e-305
Identity = 518/609 (85.06%), Postives = 559/609 (91.79%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MP  A RSMLKPPT+NG YKP SK QT   NNG ++ +  V PSTLGHYLARRLVQIG+S
Sbjct: 20  MPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSVVPSTLGHYLARRLVQIGIS 79

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+C VTFTVGGLS
Sbjct: 80  DIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLS 139

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYG--SNKILHHTIGLPDFSQELRCFQNVTCYQAV 180
           LINAIAGAYSEDLP+VCIVG PNSNEYG  + KILHHTIGLPDFSQEL+CF+NVTCYQAV
Sbjct: 140 LINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGLPDFSQELQCFRNVTCYQAV 199

Query: 181 INNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMG 240
           INNL+DAQWEID AISKCLE+SKPVYISI+CNLAAIPHPSFS++Q +PLSIS KQ+NQM 
Sbjct: 200 INNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSFSVQQLIPLSISPKQSNQMS 259

Query: 241 LEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENH 300
           LEMAVE AAQ+LN AVKP++VGGKKLRVAKAQAAFVELA+ CDYAVAVTPSAKGMFPENH
Sbjct: 260 LEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENH 319

Query: 301 RHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVF 360
            HF+GTYWG LS+AFCGETVEIADASIF GAIFDELETVG+SLSYKKNKAIIVE+D VVF
Sbjct: 320 PHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVF 379

Query: 361 PNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHI 420
           PNGP FGPILMKDFL ALAKR+KPN TAYENY RIYIP+S P  SEPGEALRVNVLFKHI
Sbjct: 380 PNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHI 439

Query: 421 QKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQA--SSDD 480
           QKML+ +M VISEAGDSWFH QKL LP+SCG+EMQ+VYASIGWSLGATLGYAQA  SS+D
Sbjct: 440 QKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSND 499

Query: 481 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGL 540
           RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ L
Sbjct: 500 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSAL 559

Query: 541 VEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKL 600
           V+AMHN NGNCWTTKV+TEEELVKAIE+AT D N+CLCFIE IVHRDDTSKELL+FGNKL
Sbjct: 560 VDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKL 619

Query: 601 AATNSRPPK 606
           A  NSRPP+
Sbjct: 620 AEMNSRPPE 628

BLAST of CmaCh03G003390 vs. NCBI nr
Match: XP_022978120.1 (pyruvate decarboxylase 1-like [Cucurbita maxima])

HSP 1 Score: 1222.6 bits (3162), Expect = 0.0e+00
Identity = 605/605 (100.00%), Postives = 605/605 (100.00%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS
Sbjct: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS
Sbjct: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180
           LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN
Sbjct: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180

Query: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 240
           NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE
Sbjct: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 240

Query: 241 MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300
           MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH
Sbjct: 241 MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300

Query: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360
           FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN
Sbjct: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360

Query: 361 GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420
           GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK
Sbjct: 361 GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420

Query: 421 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL 480
           MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL
Sbjct: 421 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL 480

Query: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 540
           CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM
Sbjct: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 540

Query: 541 HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600
           HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN
Sbjct: 541 HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600

Query: 601 SRPPK 606
           SRPPK
Sbjct: 601 SRPPK 605

BLAST of CmaCh03G003390 vs. NCBI nr
Match: XP_023544035.1 (pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 598/605 (98.84%), Postives = 602/605 (99.50%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS
Sbjct: 20  MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 79

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+CVVTFTVGGLS
Sbjct: 80  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACVVTFTVGGLS 139

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180
           LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN
Sbjct: 140 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 199

Query: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 240
           NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE
Sbjct: 200 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 259

Query: 241 MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300
           MAVEMAAQ+LNTAVKP+MVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH
Sbjct: 260 MAVEMAAQLLNTAVKPVMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 319

Query: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360
           FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN
Sbjct: 320 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 379

Query: 361 GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420
           GP FGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK
Sbjct: 380 GPSFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 439

Query: 421 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL 480
           MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQAS +DRVVL
Sbjct: 440 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASPEDRVVL 499

Query: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 540
           CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM
Sbjct: 500 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 559

Query: 541 HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600
           HNQ GNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN
Sbjct: 560 HNQTGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 619

Query: 601 SRPPK 606
           SRPPK
Sbjct: 620 SRPPK 624

BLAST of CmaCh03G003390 vs. NCBI nr
Match: XP_022949991.1 (pyruvate decarboxylase 1-like [Cucurbita moschata] >KAG6603573.1 Pyruvate decarboxylase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033762.1 Pyruvate decarboxylase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 593/605 (98.02%), Postives = 600/605 (99.17%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS
Sbjct: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+CVVTFTVGGLS
Sbjct: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACVVTFTVGGLS 120

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180
           LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN
Sbjct: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVIN 180

Query: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLE 240
           NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQ+NQMGLE
Sbjct: 181 NLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQSNQMGLE 240

Query: 241 MAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300
           MAVEMAAQ+LNTAVKP+MVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH
Sbjct: 241 MAVEMAAQLLNTAVKPVMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRH 300

Query: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360
           FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN
Sbjct: 301 FIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPN 360

Query: 361 GPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420
           GP FGP+LMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK
Sbjct: 361 GPSFGPVLMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQK 420

Query: 421 MLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVL 480
           MLSGNMAVISEAGDSWFHCQ LELPRSCGFEMQLVYASIGWSLGATLGYAQAS +DRVVL
Sbjct: 421 MLSGNMAVISEAGDSWFHCQNLELPRSCGFEMQLVYASIGWSLGATLGYAQASPEDRVVL 480

Query: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAM 540
           CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVEAM
Sbjct: 481 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWNYTALVEAM 540

Query: 541 HNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600
           HNQ GNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN
Sbjct: 541 HNQTGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATN 600

Query: 601 SRPPK 606
           SRPPK
Sbjct: 601 SRPPK 605

BLAST of CmaCh03G003390 vs. NCBI nr
Match: TYK10923.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1058.9 bits (2737), Expect = 1.6e-305
Identity = 519/609 (85.22%), Postives = 559/609 (91.79%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           MP  A RSMLKPPT+NG YKP SK QT   NNG ++ +  V PSTLGHYLARRLVQIG+S
Sbjct: 20  MPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSVVPSTLGHYLARRLVQIGIS 79

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+C VTFTVGGLS
Sbjct: 80  DIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLS 139

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYG--SNKILHHTIGLPDFSQELRCFQNVTCYQAV 180
           LINAIAGAYSEDLP+VCIVG PNSNEYG  + KILHHTIGLPDFSQEL+CF+NVTCYQAV
Sbjct: 140 LINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGLPDFSQELQCFRNVTCYQAV 199

Query: 181 INNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMG 240
           INNL+DAQWEID AISKCLE+SKPVYISI+CNLAAIPHPSFSM+Q +PLSIS KQ+NQM 
Sbjct: 200 INNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSFSMQQLIPLSISPKQSNQMS 259

Query: 241 LEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENH 300
           LEMAVE AAQ+LN AVKP++VGGKKLRVAKAQAAFVELA+ CDYAVAVTPSAKGMFPENH
Sbjct: 260 LEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENH 319

Query: 301 RHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVF 360
            HF+GTYWG LS+AFCGETVEIADASIF GAIFDELETVG+SLSYKKNKAIIVE+D VVF
Sbjct: 320 PHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVF 379

Query: 361 PNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHI 420
           PNGP FGPILMKDFL ALAKR+KPN TAYENY RIYIP+S P  SEPGEALRVNVLFKHI
Sbjct: 380 PNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHI 439

Query: 421 QKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQA--SSDD 480
           QKML+ +M VISEAGDSWFH QKL LP+SCG+EMQ+VYASIGWSLGATLGYAQA  SS+D
Sbjct: 440 QKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSND 499

Query: 481 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGL 540
           RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ L
Sbjct: 500 RVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSAL 559

Query: 541 VEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKL 600
           V+AMHN NGNCWTTKV+TEEELVKAIE+AT D N+CLCFIE IVHRDDTSKELL+FGNKL
Sbjct: 560 VDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKL 619

Query: 601 AATNSRPPK 606
           A  NSRPP+
Sbjct: 620 AEMNSRPPE 628

BLAST of CmaCh03G003390 vs. NCBI nr
Match: XP_038882338.1 (pyruvate decarboxylase 1-like, partial [Benincasa hispida])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.6e-305
Identity = 515/605 (85.12%), Postives = 554/605 (91.57%), Query Frame = 0

Query: 1   MPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVS 60
           +P  A RSMLKPPT NG YKP SK QT N NN  ++ E  V PSTLGHYLARRLVQIGVS
Sbjct: 20  IPTLATRSMLKPPTPNGIYKPHSKAQTINTNNEPQMAEPTVIPSTLGHYLARRLVQIGVS 79

Query: 61  DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLS 120
           DIFSVPGDSNLVLLDYFVAE GLNLVGCCSELNAGYAADGYAR RG+G+CVVTFTVGGLS
Sbjct: 80  DIFSVPGDSNLVLLDYFVAEIGLNLVGCCSELNAGYAADGYARHRGIGACVVTFTVGGLS 139

Query: 121 LINAIAGAYSEDLPLVCIVGAPNSNEYGSN-KILHHTIGLPDFSQELRCFQNVTCYQAVI 180
           LIN+IAGAYSEDLP+VCIVG PNSNEYGSN KILHHTIGLPDF+QEL+CF+NVTCYQAVI
Sbjct: 140 LINSIAGAYSEDLPVVCIVGGPNSNEYGSNKKILHHTIGLPDFTQELQCFKNVTCYQAVI 199

Query: 181 NNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGL 240
           NNLEDAQWEID AISKCLE+SKPVYISI+CNL AIPHPSFS +Q +P+S S+KQ+NQM L
Sbjct: 200 NNLEDAQWEIDCAISKCLEESKPVYISIACNLVAIPHPSFSAQQLIPISYSAKQSNQMAL 259

Query: 241 EMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHR 300
           EMAVE AA++LN+AVKP++VGGKKLR AKAQA FVELA+ C+YAVAVTPSAKGMFPE H 
Sbjct: 260 EMAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHP 319

Query: 301 HFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFP 360
           HFIGTYWG LS+AFCGETVEIADASIF GA+FDELETVG+SLSYKKN AII+E+D VVFP
Sbjct: 320 HFIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFP 379

Query: 361 NGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQ 420
           NGP FGPILMKDFL ALAKRLKPN  AYENY RIYIP+S P +SEPGEALRVNVLFKHIQ
Sbjct: 380 NGPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQ 439

Query: 421 KMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVV 480
           KMLS NM VISEAGDSWFHCQKLELPRSCG+EMQLVYASIGWSLGATLGYAQAS DDRVV
Sbjct: 440 KMLSSNMVVISEAGDSWFHCQKLELPRSCGYEMQLVYASIGWSLGATLGYAQASLDDRVV 499

Query: 481 LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEA 540
           LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYT LV+A
Sbjct: 500 LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDA 559

Query: 541 MHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAAT 600
           MHN NGNCWT KV+TEEELVKAIE+A GD+ +CLCFIEAIVH+DDTSKELL+FGNKLAA 
Sbjct: 560 MHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAI 619

Query: 601 NSRPP 605
           NSRPP
Sbjct: 620 NSRPP 624

BLAST of CmaCh03G003390 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 810.1 bits (2091), Expect = 1.2e-234
Identity = 392/583 (67.24%), Postives = 470/583 (80.62%), Query Frame = 0

Query: 31  NNGVEIVE-------SMVTP--STLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEK 90
           N GV  V+       + V+P  +TLG YLARRLV+IGV+D+FSVPGD NL LLD+ +AE 
Sbjct: 23  NGGVSTVQNTSPLHSTTVSPCDATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEP 82

Query: 91  GLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVGA 150
            L L+GCC+ELNAGYAADGYAR RGVG+CVVTFTVGGLS++NAIAGAYSE+LPL+CIVG 
Sbjct: 83  NLKLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGG 142

Query: 151 PNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSK 210
           PNSN+YG+N+ILHHTIGLPDF+QELRCFQ VTC+QAVINNLE+A   IDTAIS  L++SK
Sbjct: 143 PNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESK 202

Query: 211 PVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVGG 270
           PVYISISCNL AIP P+FS +  +P  +  K +NQ+GL+ AVE AA+ LN AVKP++VGG
Sbjct: 203 PVYISISCNLPAIPLPTFS-RHPVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGG 262

Query: 271 KKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIA 330
            K+RVAKA  AFVELAD   Y +AV PSAKG  PE+H+HFIGTYWG++S+AFC E VE A
Sbjct: 263 PKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESA 322

Query: 331 DASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRLK 390
           DA +FAG IF++  +VGYSL  KK KAIIV+ DRV   NGP FG +LMKDFL  LAKR+K
Sbjct: 323 DAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIK 382

Query: 391 PNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQK 450
            N T+YENY RIY+P+  P +  P E+LRVNVLF+HIQ MLS   AV++E GDSWF+CQK
Sbjct: 383 HNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQK 442

Query: 451 LELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRLG 510
           L+LP  CG+E Q+ Y SIGWS+GATLGYAQA  + RV+ CIGDGSFQ+  QD+STM+R G
Sbjct: 443 LKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCG 502

Query: 511 QRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVKA 570
           Q+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YT  VEA+HN  G CWT KV  EEELVKA
Sbjct: 503 QKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKA 562

Query: 571 IEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPP 605
           I  AT ++ E  CFIE IVH+DDTSKELL++G++++A NSRPP
Sbjct: 563 INTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of CmaCh03G003390 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 809.7 bits (2090), Expect = 1.6e-234
Identity = 391/584 (66.95%), Postives = 471/584 (80.65%), Query Frame = 0

Query: 21  PQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAE 80
           P S +  N C+            +TLG +LARRLVQ GV+D+FSVPGD NL LLD+ +AE
Sbjct: 34  PSSAITINYCD------------ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAE 93

Query: 81  KGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVG 140
             LNL+GCC+ELNAGYAADGYAR RGVG+CVVTFTVGGLS++NAIAGAYSE+LPL+CIVG
Sbjct: 94  PDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVG 153

Query: 141 APNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQS 200
            PNSN+YG+N+ILHHTIGLPDFSQELRCFQ VTCYQAV+NNL+DA  +ID AIS  L++S
Sbjct: 154 GPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKES 213

Query: 201 KPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVG 260
           KPVYIS+SCNLAAIPH +FS +  +P S++ + +N+MGLE AVE   + LN AVKP+MVG
Sbjct: 214 KPVYISVSCNLAAIPHHTFS-RDPVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVG 273

Query: 261 GKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEI 320
           G KLRVAKA  AFVELAD   YA+A+ PSAKG  PE+H HFIGTYWG++S+ FC E VE 
Sbjct: 274 GPKLRVAKACDAFVELADASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVES 333

Query: 321 ADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRL 380
           ADA IFAG IF++  +VGYSL  KK KAI+V+ DR+   NGP FG ILM DF R L+KR+
Sbjct: 334 ADAYIFAGPIFNDYSSVGYSLLLKKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRV 393

Query: 381 KPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQ 440
           K N TAYENY RI++P+  P + E  E LRVN +F+HIQKMLS   AVI+E GDSWF+CQ
Sbjct: 394 KRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQ 453

Query: 441 KLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRL 500
           KL+LP+ CG+E Q+ Y SIGWS+GATLGYAQAS + RV+  IGDGSFQ+  QD+STMLR 
Sbjct: 454 KLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRN 513

Query: 501 GQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVK 560
           GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YTGLV+A+HN  GNCWT KV  EEELV+
Sbjct: 514 GQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVE 573

Query: 561 AIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPP 605
           AI  AT +K +CLCFIE I+H+DDTSKELL++G++++A NSRPP
Sbjct: 574 AITTATTEKKDCLCFIEVILHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of CmaCh03G003390 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 804.7 bits (2077), Expect = 5.2e-233
Identity = 388/594 (65.32%), Postives = 474/594 (79.80%), Query Frame = 0

Query: 11  KPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVSDIFSVPGDSN 70
           KP T +    P + + T   +  +    +  + STLG +L+RRLVQ GV+D+FSVPGD N
Sbjct: 12  KPTTGDIGSPPSNAVATIQDSAPI----TTTSESTLGRHLSRRLVQAGVTDVFSVPGDFN 71

Query: 71  LVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSLINAIAGAYS 130
           L LLD+ +AE  LN +GCC+ELNAGYAADGYAR RGVG+CVVTFTVGGLS++NAIAGAYS
Sbjct: 72  LTLLDHLIAEPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYS 131

Query: 131 EDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINNLEDAQWEID 190
           E+LP++CIVG PNSN++G+N+ILHHTIGLPDFSQELRCFQ VTCYQAV+NNLEDA  +ID
Sbjct: 132 ENLPVICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQID 191

Query: 191 TAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEMAVEMAAQIL 250
            AI+  L++SKPVYISISCNLAA PHP+F+ +  +P  ++ + +N MGLE AVE   + L
Sbjct: 192 KAIATALKESKPVYISISCNLAATPHPTFA-RDPVPFDLTPRMSNTMGLEAAVEATLEFL 251

Query: 251 NTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHFIGTYWGSLS 310
           N AVKP+MVGG KLRVAKA  AF+ELAD   Y +AV PS KG+ PENH HFIGTYWG++S
Sbjct: 252 NKAVKPVMVGGPKLRVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVS 311

Query: 311 SAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNGPIFGPILMK 370
           + FC E VE ADA IFAG IF++  +VGYSL  KK KAIIV  DRVV  NGP FG +LM 
Sbjct: 312 TPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMS 371

Query: 371 DFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVIS 430
           DF R LAKR+K N TAYENY RI++P+  P + +PGE LRVN +F+HIQKMLS   AVI+
Sbjct: 372 DFFRELAKRVKRNETAYENYERIFVPEGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIA 431

Query: 431 EAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMA 490
           E GDSWF+CQKL+LP+ CG+E Q+ Y SIGWS+GATLGYAQA+ + RV+  IGDGSFQ+ 
Sbjct: 432 ETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVT 491

Query: 491 PQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMHNQNGNCWTT 550
            QD+STM+R GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YTGLV+A+HN  G CWTT
Sbjct: 492 AQDISTMIRNGQKAIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTT 551

Query: 551 KVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNSRPP 605
           KV  EEELV+AI+ AT +K + LCFIE IVH+DDTSKELL++G++++A N RPP
Sbjct: 552 KVRYEEELVEAIKTATTEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPP 600

BLAST of CmaCh03G003390 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 788.5 bits (2035), Expect = 3.8e-228
Identity = 378/561 (67.38%), Postives = 456/561 (81.28%), Query Frame = 0

Query: 44  STLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYAR 103
           +TLG +L+RRLVQ GV+DIF+VPGD NL LLD  +A   LN +GCC+ELNAGYAADGYAR
Sbjct: 30  ATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNNIGCCNELNAGYAADGYAR 89

Query: 104 RRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFS 163
            RGVG+CVVTFTVGGLS++NAIAGAYSE+LP++CIVG PNSN++G+N+ILHHTIGLPDFS
Sbjct: 90  SRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFS 149

Query: 164 QELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQ 223
           QELRCFQ VTCYQAV+N+LEDA  +ID AI+  L +SKPVYISISCNLAAIPHP+F+   
Sbjct: 150 QELRCFQTVTCYQAVVNHLEDAHEQIDKAIATALRESKPVYISISCNLAAIPHPTFASYP 209

Query: 224 FLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYA 283
            +P  ++ + +N+  LE AVE   + LN AVKP+MVGG KLRVAKA+ AFVELAD   Y 
Sbjct: 210 -VPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVMVGGPKLRVAKARDAFVELADASGYP 269

Query: 284 VAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSY 343
           VAV PSAKG  PENH HFIGTYWG++S+ FC E VE ADA IFAG IF++  +VGYSL  
Sbjct: 270 VAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAYIFAGPIFNDYSSVGYSLLL 329

Query: 344 KKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQS 403
           KK KAIIV  D VV  NGP FG + M +F R LAKR+KPN TAYENY RI++P+  P + 
Sbjct: 330 KKEKAIIVHPDSVVVANGPTFGCVRMSEFFRELAKRVKPNKTAYENYHRIFVPEGKPLKC 389

Query: 404 EPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSL 463
           +P E LR+N +F+HIQKMLS   AVI+E GDSWF+CQKL+LP+ CG+E Q+ Y SIGWS+
Sbjct: 390 KPREPLRINAMFQHIQKMLSNETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSV 449

Query: 464 GATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDG 523
           GATLGYAQA+ + RV+  IGDGSFQ+  QD+STM+R GQ+ IIFLINNGGYTIEVEIHDG
Sbjct: 450 GATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMIRNGQKTIIFLINNGGYTIEVEIHDG 509

Query: 524 PYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRD 583
           PYNVIKNW+YTGLV+A+HN  G CWTTKV  EEELV+AI  AT +K + LCFIE IVH+D
Sbjct: 510 PYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAINTATLEKKDSLCFIEVIVHKD 569

Query: 584 DTSKELLDFGNKLAATNSRPP 605
           DTSKELL++G++++A N RPP
Sbjct: 570 DTSKELLEWGSRVSAANGRPP 589

BLAST of CmaCh03G003390 vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 86.7 bits (213), Expect = 7.2e-17
Identity = 113/508 (22.24%), Postives = 206/508 (40.55%), Query Frame = 0

Query: 50  LARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRG-VG 109
           L   L + GV  +F+ PG +++ +         +  V    E    +AA+GYAR  G  G
Sbjct: 103 LVEALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPG 162

Query: 110 SCVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQE--L 169
            C+ T   G  +L++ +A A  + +PLV I G          ++    IG   F +   +
Sbjct: 163 ICIATSGPGATNLVSGLADALLDSVPLVAITG----------QVPRRMIGTDAFQETPIV 222

Query: 170 RCFQNVTCYQAVINNLEDAQWEIDTAISKCLE-QSKPVYISISCNL---AAIPHPSFSMK 229
              +++T +  ++ ++ED    I+ A       +  PV + +  ++    AIP+   +M+
Sbjct: 223 EVTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMR 282

Query: 230 QFLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIM-VGGKKLRVAKAQAAFVELADVCD 289
               +S   K      LE  V    ++++ + KP++ VGG  L  +     FVEL  +  
Sbjct: 283 LPGYMSRMPKPPEDSHLEQIV----RLISESKKPVLYVGGGCLNSSDELGRFVELTGI-- 342

Query: 290 YAVAVTPSAKGMFP---ENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVG 349
             VA T    G +P   E   H +G +     + +    VE +D  +  G  FD+  T  
Sbjct: 343 -PVASTLMGLGSYPCDDELSLHMLGMH----GTVYANYAVEHSDLLLAFGVRFDDRVTGK 402

Query: 350 YSLSYKKNKAIIVESDRVVF-----PNGPIFGPIL-----MKDFLRALAKRLKPNPTAYE 409
                 + K + ++ D         P+  + G +      M   L   A+ LK +   + 
Sbjct: 403 LEAFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWR 462

Query: 410 NYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKMLSGNMAVISEAGD-SWFHCQKLELPRS 469
           N   +   K   +    GEA+      K + ++  G   + +  G    +  Q     + 
Sbjct: 463 NELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAAQFYNYKKP 522

Query: 470 CGFEMQLVYASIGWSLGATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIF 529
             +       ++G+ L A +G + A+ D  VV   GDGSF M  Q+L+T+        + 
Sbjct: 523 RQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVKVL 582

Query: 530 LINNGGYTIEVEIHDGPYNVIKNWDYTG 536
           L+NN    + ++  D  Y   +   + G
Sbjct: 583 LLNNQHLGMVMQWEDRFYKANRAHTFLG 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2Y5L93.2e-23565.82Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=... [more]
Q0DHF69.2e-23565.82Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 S... [more]
Q9FFT41.7e-23367.24Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1[more]
O826472.3e-23366.95Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1[more]
P285162.3e-23368.09Pyruvate decarboxylase 1 OS=Zea mays OX=4577 GN=PDC1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IP680.0e+00100.00Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111478194 PE=3 SV=1[more]
A0A6J1GDK70.0e+0098.02Pyruvate decarboxylase OS=Cucurbita moschata OX=3662 GN=LOC111453217 PE=3 SV=1[more]
A0A5D3CG967.9e-30685.22Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A5A7T4K42.3e-30585.06Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3CL302.3e-30585.06Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502191 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022978120.10.0e+00100.00pyruvate decarboxylase 1-like [Cucurbita maxima][more]
XP_023544035.10.0e+0098.84pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022949991.10.0e+0098.02pyruvate decarboxylase 1-like [Cucurbita moschata] >KAG6603573.1 Pyruvate decarb... [more]
TYK10923.11.6e-30585.22pyruvate decarboxylase 1-like [Cucumis melo var. makuwa][more]
XP_038882338.13.6e-30585.12pyruvate decarboxylase 1-like, partial [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G54960.11.2e-23467.24pyruvate decarboxylase-2 [more]
AT4G33070.11.6e-23466.95Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G01320.15.2e-23365.32Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G01330.13.8e-22867.38pyruvate decarboxylase-3 [more]
AT3G48560.17.2e-1722.24chlorsulfuron/imidazolinone resistant 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.970coord: 400..592
e-value: 2.1E-52
score: 179.2
NoneNo IPR availableGENE3D3.40.50.970coord: 41..219
e-value: 3.8E-58
score: 198.1
NoneNo IPR availableGENE3D3.40.50.1220coord: 233..395
e-value: 2.7E-35
score: 123.4
NoneNo IPR availablePIRSRPIRSR036565-2PIRSR036565-2coord: 45..589
e-value: 7.8E-166
score: 550.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 22..605
NoneNo IPR availableCDDcd07038TPP_PYR_PDC_IPDC_likecoord: 49..209
e-value: 2.0146E-88
score: 269.364
NoneNo IPR availableCDDcd02005TPP_PDC_IPDCcoord: 408..590
e-value: 1.0702E-75
score: 237.047
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePIRSFPIRSF036565Pyruvt_ip_decrbcoord: 40..602
e-value: 4.6E-193
score: 640.4
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 22..605
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 45..213
e-value: 5.7E-33
score: 113.9
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 244..355
e-value: 1.7E-18
score: 66.7
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 453..573
e-value: 1.7E-14
score: 53.9
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 410..602
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 45..219
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 227..386

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G003390.1CmaCh03G003390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004737 pyruvate decarboxylase activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity