CmaCh02G011110 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh02G011110
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSuccinate dehydrogenase assembly factor 4, mitochondrial
LocationCma_Chr02: 6604060 .. 6604392 (-)
RNA-Seq ExpressionCmaCh02G011110
SyntenyCmaCh02G011110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGCAATCTCACCCGTCTATTCTCGCCGCCTAGTCTCACCGCTCTATCGGCGGTGAATCGCGTCACTTCCACTTCGGTGAGTCGACTCGTCTCCTCCTCGGCGAATCAACAACCTTCTCGATTGGAGCAAGGTGTTCCCGGCGAGGAGCAACGAGAAGCTGGAAAAGGAAGTTTGCCGGATCAACACGAGGCTGCCGGAGATGATCGAGATAGTGAAGATGAGGGAGCTGATGTGAACAAAGCGACTGGCGAGGTCGGTGGACCTAAAGGTCCCGAGCCAACTCGCTATGGCGATTGGGAAAGAAACGGTCGGTGCTACGATTTTTGA

mRNA sequence

ATGTCGAGCAATCTCACCCGTCTATTCTCGCCGCCTAGTCTCACCGCTCTATCGGCGGTGAATCGCGTCACTTCCACTTCGGTGAGTCGACTCGTCTCCTCCTCGGCGAATCAACAACCTTCTCGATTGGAGCAAGGTGTTCCCGGCGAGGAGCAACGAGAAGCTGGAAAAGGAAGTTTGCCGGATCAACACGAGGCTGCCGGAGATGATCGAGATAGTGAAGATGAGGGAGCTGATGTGAACAAAGCGACTGGCGAGGTCGGTGGACCTAAAGGTCCCGAGCCAACTCGCTATGGCGATTGGGAAAGAAACGGTCGGTGCTACGATTTTTGA

Coding sequence (CDS)

ATGTCGAGCAATCTCACCCGTCTATTCTCGCCGCCTAGTCTCACCGCTCTATCGGCGGTGAATCGCGTCACTTCCACTTCGGTGAGTCGACTCGTCTCCTCCTCGGCGAATCAACAACCTTCTCGATTGGAGCAAGGTGTTCCCGGCGAGGAGCAACGAGAAGCTGGAAAAGGAAGTTTGCCGGATCAACACGAGGCTGCCGGAGATGATCGAGATAGTGAAGATGAGGGAGCTGATGTGAACAAAGCGACTGGCGAGGTCGGTGGACCTAAAGGTCCCGAGCCAACTCGCTATGGCGATTGGGAAAGAAACGGTCGGTGCTACGATTTTTGA

Protein sequence

MSSNLTRLFSPPSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGSLPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF
Homology
BLAST of CmaCh02G011110 vs. ExPASy Swiss-Prot
Match: Q8BTE0 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Mus musculus OX=10090 GN=Sdhaf4 PE=3 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 2.0e-07
Identity = 42/101 (41.58%), Postives = 54/101 (53.47%), Query Frame = 0

Query: 24  TSTSVSRLVSSSANQQPSRL---------EQGVPGEEQREAGKGSLP----DQHEAAGDD 83
           T+ SVSR+       +PS L         ++G P   ++   K  LP    D  E + ++
Sbjct: 4   TTLSVSRMTFVWRAARPSLLNHSLRKMSYQEGKPEPAKQALKKSKLPLGRFDSLEDSPEE 63

Query: 84  RDS-EDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 111
           R+  +    DVN  T E GGPKGPEPTRYGDWER GRC DF
Sbjct: 64  REPLQKFPDDVNPVTKEKGGPKGPEPTRYGDWERKGRCIDF 104

BLAST of CmaCh02G011110 vs. ExPASy Swiss-Prot
Match: Q9VLU6 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Sirup PE=3 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 4.5e-07
Identity = 23/30 (76.67%), Postives = 24/30 (80.00%), Query Frame = 0

Query: 81  NKATGEVGGPKGPEPTRYGDWERNGRCYDF 111
           N  TGE+GGP GPEPTRYGDWER GR  DF
Sbjct: 89  NPYTGEIGGPAGPEPTRYGDWERKGRVSDF 118

BLAST of CmaCh02G011110 vs. ExPASy Swiss-Prot
Match: Q5VUM1 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Homo sapiens OX=9606 GN=SDHAF4 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 2.3e-06
Identity = 45/113 (39.82%), Postives = 52/113 (46.02%), Query Frame = 0

Query: 3   SNLTRLFSPPSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGSLPD 62
           S L  L S  S TA  A          R  SSS   +   ++Q +         K  LP+
Sbjct: 4   SRLPWLLSWVSATAWRAARSPLLCHSLRKTSSSQGGKSELVKQSLK--------KPKLPE 63

Query: 63  QHEAAGDDRDSEDEGA-----DVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 111
               A +D   E E       DVN  T E GGP+GPEPTRYGDWER GRC DF
Sbjct: 64  GRFDAPEDSHLEKEPLEKFPDDVNPVTKEKGGPRGPEPTRYGDWERKGRCIDF 108

BLAST of CmaCh02G011110 vs. ExPASy Swiss-Prot
Match: Q54Y25 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=DDB_G0278459 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 2.5e-05
Identity = 34/99 (34.34%), Postives = 50/99 (50.51%), Query Frame = 0

Query: 14  LTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGE--EQREAGKGSLPDQHEAAGDDR 73
           L A    N++  +  S  ++++ N   +     +  +  E  +  +  L D  E    + 
Sbjct: 14  LNATKQTNKINCSFYSTTINNNNNNNNNNNNNNISNKKAEMSKENQQLLQDLEE----EF 73

Query: 74  DSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 111
           D ++    VN  T E+GGPKGPEPTRY DWERNGR  DF
Sbjct: 74  DEDEFKPYVNPITKEIGGPKGPEPTRYNDWERNGRVSDF 108

BLAST of CmaCh02G011110 vs. TAIR 10
Match: AT5G67490.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1674 (InterPro:IPR012875); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 56.6 bits (135), Expect = 1.4e-08
Identity = 43/104 (41.35%), Postives = 54/104 (51.92%), Query Frame = 0

Query: 21  NRVTSTSVSRLVSSSANQQPSR-LEQGVPGEEQ-----REAGKGSLPDQHEAAGDDRDSE 80
           N V   + SR VSS   +  SR L  G P   Q     ++  +   PDQ+         E
Sbjct: 5   NIVRLIAPSRSVSSRFLEPVSRFLSSGTPPPPQAPSPNQDLNRDVKPDQNLQQNLQMQKE 64

Query: 81  DE--------GADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 111
           +E        G  VN+ TGE+GGP+GPEPTRYGDWE+ GRC DF
Sbjct: 65  EEEEGEGGGGGEFVNEDTGEIGGPRGPEPTRYGDWEQRGRCSDF 108

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8BTE02.0e-0741.58Succinate dehydrogenase assembly factor 4, mitochondrial OS=Mus musculus OX=1009... [more]
Q9VLU64.5e-0776.67Succinate dehydrogenase assembly factor 4, mitochondrial OS=Drosophila melanogas... [more]
Q5VUM12.3e-0639.82Succinate dehydrogenase assembly factor 4, mitochondrial OS=Homo sapiens OX=9606... [more]
Q54Y252.5e-0534.34Succinate dehydrogenase assembly factor 4, mitochondrial OS=Dictyostelium discoi... [more]
Match NameE-valueIdentityDescription
AT5G67490.11.4e-0841.35unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1674... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012875Succinate dehydrogenase assembly factor 4PFAMPF07896DUF1674coord: 74..110
e-value: 5.4E-18
score: 65.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..81
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..110
NoneNo IPR availablePANTHERPTHR28524SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIALcoord: 1..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G011110.1CmaCh02G011110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008652 cellular amino acid biosynthetic process
biological_process GO:0009064 glutamine family amino acid metabolic process
biological_process GO:0034553 mitochondrial respiratory chain complex II assembly
cellular_component GO:0005739 mitochondrion
molecular_function GO:0043167 ion binding
molecular_function GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors