CmaCh02G010950 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh02G010950
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionmyosin IB heavy chain-like
LocationCma_Chr02: 6519847 .. 6522219 (+)
RNA-Seq ExpressionCmaCh02G010950
SyntenyCmaCh02G010950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTACGAGAGAGACCCATTTTTTCTCGGCGAGTCCAACTTCGAACAACAACAATGGAATCCAGATCCCGGAGGCGGGCCCTCATCGACGACAATTCTCCTTCCGCCGGAGTTGTGGAACGACGCGACGCCGTCGGGACCGCCGACGATGTGCAGGGACTGAAATTTCGACCCTCCGATGGGAATGTGACCGAAGATCAGGATCTCTTCATGGGCATCAAGGTCCGTCGAAAAGACTCGCTTCATAATGATTACAAGGGGGATTACATTGATGTTCCCTCGAATCAATTCTTGATGAAAATCCTGCAAAAACAAGGTTAAATTTGCACATGCCGCGTTGATATTATATATTATTTACGGTGTTTTGATTGTCTCCGGACTGGAATTGAATTTCGTTTGCTTTTGTAATTCGCTCACGTATCCATGTATTTCGTGTCTGAAATTCGTAAATTATATTTGATTGATTATAGTTTGTTCGTCCTTCTTTGTCAAATTGTATGTCGATGATAGTTTTCCATCTCCAGAATTGTGAATCTATTGCTCTTATGATGTTTAATAACTCTTCTTTCTCTTGTCCCTTACTTTTTCTATGCAATCAAATGAAAATGGTGTTTGATTTTTAGGATGATTGCAAGGTAATGATGGAAATATACACGCATATGAAATTATTATCTATTTGGACACTATGTATTGTTTAATTCCTAAACATATAATTTATTCCTATGCATTGTGTAGTTTCCTTTTTGGATTTTTGAATGAATAGGATCATTTCAGCTGGCTATTAAGCATTTTGGAGAGTATTTAGCAGAGTGAAGACGAGAGATTTTGTTGGATTTACTTTCTTGTATCTTCTAAACTCCATCTTAGAATAAGACATGAAATTTATTTGGTTTAACTCTTTAAGTATAATGAGGAAGAAAATAGTATCCTCGGGTGTGTTAATGTCTCTTCGAACGGCCCCTACTCTTGCAGAATATTACGGTTTTTACTGTGTTCGTATCAGCCATTTTGAACTGATGCATCGTTCATACACGTCTTCTTTTGAATTCTATATCAAGCCATAACCAAGTTCTTCCATGCTTTTTATCCCTGCAGGTGAAGAGAGTGTGCTTTTTGCAGACAAAGTTTTGAAGTTCACTGCTTCCGGGAAGATGAAAAGACGGATTCTTTTAATAACAGAGTTTGCCATTTATATCATTGACCCAGAAACTGATACCCTTAAAAGGAGAGTTGCCCTGGCAGCTGTGGATAAGATATGTTTAAGTGAACTAAGTGATAACTTTTATGCAATTGTAATTCCAACCGAGTATGATATACTGATGGCAAGTACTCGGAAAACAGAGATCGTAACAGTTTTGGTTGACGCCTTTAAGAGTTCCTCTGATTATGAACTTGAGATTGTATTCTCCAACAGGTAATGGACATCATCTTCCCCATTTATCGTTCCCATTATGAACCGATATTTTCATTGTTATATGATTGTAGTATGTAAATCAGTTAACGAGATTTTGCTTCGGATATTTTGAAAGTTTTTCATTTCATTTTGATTTAGTCACCATTTGAATATCTATTTCTTCTTGTTGAGTTGTAGTGTTTAAAGACGAGGATATTTGTGTTTTTTTCTAAATGTTTGTTATCCCTCTTTTCTTTCCTTTTTTAGGTTTGTGTACAGTGCAGGTGGTGAACTGATAAAAGAAGTTCAATTCGAGGAAGTCGAAGGTATGAATATATCTTTTTAAGTCGGAATTTAGCATTCTCGTTTATTTTGTTCTTGTTTCTCGTACTCTGTTTTCGAAGATAGATTTGTTTCCCTTCTCAAAGAATTGGTAAAAGAAGTACTCTCTTCTGTTTTCCCTTTATATTGGGGAAAAAAACACATTATTTTGTTCTTTTTTCACCTTATTCTCTCAAACTTTACATTTATTGAAATTATTTTCAAATGTTGTATAAAAAATTGGGAACATTTCTTGTTACCAAATGGAAAGCAAGGAAGAGTTACCAAAATACAAATGTATCTGAAGAACCAAAAACATTACTAAAAGTGTCTGGTACTGGTAATAATATGCAGGTGGTGTGAAAACAAGAATTTCGAGGAAGGAGGAGTGATTTATGACTGGTTTATTGGTCAGCCTCTTCTTTTATATTTGATCAAATTGATGATTGGAATTGGATCTTGTAGATTCTTGAAGTATTTGAATCTGTGTATAGAAATGGATCGAAAGTTGTAGCTTTTGCATATACATGCCCTTCTTTTATTCCTTCTATTTTCTCTTCGTTTCTCCGTTTTGTAAGAGAACATAGCATTCGACTTCTTAGCCAAATTCTATTCTATTGTCGATATCATTCTATTATCGATATCAAACCGGACTTCGACT

mRNA sequence

ACTACGAGAGAGACCCATTTTTTCTCGGCGAGTCCAACTTCGAACAACAACAATGGAATCCAGATCCCGGAGGCGGGCCCTCATCGACGACAATTCTCCTTCCGCCGGAGTTGTGGAACGACGCGACGCCGTCGGGACCGCCGACGATGTGCAGGGACTGAAATTTCGACCCTCCGATGGGAATGTGACCGAAGATCAGGATCTCTTCATGGGCATCAAGGTCCGTCGAAAAGACTCGCTTCATAATGATTACAAGGGGGATTACATTGATGTTCCCTCGAATCAATTCTTGATGAAAATCCTGCAAAAACAAGGTGAAGAGAGTGTGCTTTTTGCAGACAAAGTTTTGAAGTTCACTGCTTCCGGGAAGATGAAAAGACGGATTCTTTTAATAACAGAGTTTGCCATTTATATCATTGACCCAGAAACTGATACCCTTAAAAGGAGAGTTGCCCTGGCAGCTGTGGATAAGATATGTTTAAGTGAACTAAGTGATAACTTTTATGCAATTGTAATTCCAACCGAGTATGATATACTGATGGCAAGTACTCGGAAAACAGAGATCGTAACAGTTTTGGTTGACGCCTTTAAGAGTTCCTCTGATTATGAACTTGAGATTGTATTCTCCAACAGGTTTGTGTACAGTGCAGGTGGTGAACTGATAAAAGAAGTTCAATTCGAGGAAGTCGAAGGTGGTGTGAAAACAAGAATTTCGAGGAAGGAGGAGTGATTTATGACTGGTTTATTGGTCAGCCTCTTCTTTTATATTTGATCAAATTGATGATTGGAATTGGATCTTGTAGATTCTTGAAGTATTTGAATCTGTGTATAGAAATGGATCGAAAGTTGTAGCTTTTGCATATACATGCCCTTCTTTTATTCCTTCTATTTTCTCTTCGTTTCTCCGTTTTGTAAGAGAACATAGCATTCGACTTCTTAGCCAAATTCTATTCTATTGTCGATATCATTCTATTATCGATATCAAACCGGACTTCGACT

Coding sequence (CDS)

ATGGAATCCAGATCCCGGAGGCGGGCCCTCATCGACGACAATTCTCCTTCCGCCGGAGTTGTGGAACGACGCGACGCCGTCGGGACCGCCGACGATGTGCAGGGACTGAAATTTCGACCCTCCGATGGGAATGTGACCGAAGATCAGGATCTCTTCATGGGCATCAAGGTCCGTCGAAAAGACTCGCTTCATAATGATTACAAGGGGGATTACATTGATGTTCCCTCGAATCAATTCTTGATGAAAATCCTGCAAAAACAAGGTGAAGAGAGTGTGCTTTTTGCAGACAAAGTTTTGAAGTTCACTGCTTCCGGGAAGATGAAAAGACGGATTCTTTTAATAACAGAGTTTGCCATTTATATCATTGACCCAGAAACTGATACCCTTAAAAGGAGAGTTGCCCTGGCAGCTGTGGATAAGATATGTTTAAGTGAACTAAGTGATAACTTTTATGCAATTGTAATTCCAACCGAGTATGATATACTGATGGCAAGTACTCGGAAAACAGAGATCGTAACAGTTTTGGTTGACGCCTTTAAGAGTTCCTCTGATTATGAACTTGAGATTGTATTCTCCAACAGGTTTGTGTACAGTGCAGGTGGTGAACTGATAAAAGAAGTTCAATTCGAGGAAGTCGAAGGTGGTGTGAAAACAAGAATTTCGAGGAAGGAGGAGTGA

Protein sequence

MESRSRRRALIDDNSPSAGVVERRDAVGTADDVQGLKFRPSDGNVTEDQDLFMGIKVRRKDSLHNDYKGDYIDVPSNQFLMKILQKQGEESVLFADKVLKFTASGKMKRRILLITEFAIYIIDPETDTLKRRVALAAVDKICLSELSDNFYAIVIPTEYDILMASTRKTEIVTVLVDAFKSSSDYELEIVFSNRFVYSAGGELIKEVQFEEVEGGVKTRISRKEE
Homology
BLAST of CmaCh02G010950 vs. ExPASy Swiss-Prot
Match: P19706 (Myosin heavy chain IB OS=Acanthamoeba castellanii OX=5755 GN=MIB PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.3e-16
Identity = 54/172 (31.40%), Postives = 93/172 (54.07%), Query Frame = 0

Query: 50  DLFMGIKVRRKDSLHNDYKGDYIDVPSNQFLMKILQKQG--EESVLFADKVLKFTASGKM 109
           DL  G K R++ S++  Y+ DYI+  +N  L   ++  G  +E+  F D+VL     GK 
Sbjct: 709 DLLKGKKERQRHSVNRKYEFDYINYDANYPLQDCVRSSGRDKEATAFTDQVLVLNRRGKP 768

Query: 110 KRRILLITEFAIYIIDPETDT------LKRRVALAAVDKICLSELSDNFYAIVIPTEYDI 169
           +RR L++T  A+Y    +  +      LKRR+ L  +  + LS L DN Y ++   +YD+
Sbjct: 769 ERRDLIVTNEAVYFAMRKKKSGQVVYNLKRRIPLGEIASLSLSTLQDN-YVVIHHNQYDM 828

Query: 170 LMASTRKTEIVTVLVDAFKSSSDYELEIVFSNRFVYSAGGELIKEVQFEEVE 214
           +  + +KTEIVT+L++ +K S   +L + F++   Y A     + + F + E
Sbjct: 829 VFENDKKTEIVTILMENYKMSGGRDLPVNFNDNITYKASNGAQRRLTFSKNE 879

BLAST of CmaCh02G010950 vs. ExPASy Swiss-Prot
Match: P34092 (Myosin IB heavy chain OS=Dictyostelium discoideum OX=44689 GN=myoB PE=1 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 4.2e-15
Identity = 54/170 (31.76%), Postives = 91/170 (53.53%), Query Frame = 0

Query: 51  LFMGIKVRRKDSLHNDYKGDYIDVPSNQFLMKILQK-QGEESVLFADKVLKFTASGKMKR 110
           +F   K R+++S+   +  DYID  +   L + +Q    +E V+FAD V+K     K K 
Sbjct: 724 MFKDKKERQRNSIDRKFTSDYIDFENQFGLQEAMQNAHKKERVVFADTVIKIDRRAKQKN 783

Query: 111 RILLITEFAIYIIDPE------TDTLKRRVALAAVDKICLSELSDNFYAIVIPTEYDILM 170
             +++T+ A+Y ++          TL RRV L  +  + +S LSDN     +P E+D ++
Sbjct: 784 YEMVLTDQALYFVEKSIKKKVLVHTLIRRVGLREIKGVSISTLSDNVIVFHLP-EHDQVI 843

Query: 171 ASTRKTEIVTVLVDAFKSSSDYELEIVFSNRFVYSAGGELIKEVQFEEVE 214
            + +KTEI+ VLV+ FK+     L + FS+R  Y+      KE+ F++ E
Sbjct: 844 ENDKKTEIIIVLVEYFKAIGGGSLNVQFSDRINYTLKKGEQKEISFQKSE 892

BLAST of CmaCh02G010950 vs. ExPASy Swiss-Prot
Match: P34109 (Myosin ID heavy chain OS=Dictyostelium discoideum OX=44689 GN=myoD PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 3.5e-14
Identity = 50/151 (33.11%), Postives = 80/151 (52.98%), Query Frame = 0

Query: 56  KVRRKDSLHNDYKGDYIDVPSNQFLMKILQKQGEESVLFADKVLKFTASGKMKRRILLIT 115
           K RR+ S+   Y+GDYI+   N  L  I++K G E ++F   V K+    + +RR+LL++
Sbjct: 725 KERRRLSIERPYQGDYINYRENFELKDIVKKNGNEKIMFTHAVNKYDRRSRCQRRVLLLS 784

Query: 116 EFAIYIIDPETD-------------TLKRRVALAAVDKICLSELSDNFYAIVIPTEYDIL 175
           + AIY I  E +               KRR+ LA +  + LS+LSD F  +    E+D +
Sbjct: 785 DTAIYFIATEKNKDKEDRKKRPWIYVQKRRLLLAGITSVELSKLSDGFVVLKTMNEHDQI 844

Query: 176 MASTRKTEIVTVLVDAFKSSSDYELEIVFSN 194
               RKTE +  L+ A+K+ +   L I ++N
Sbjct: 845 FECRRKTEFLGTLIKAYKTGT---LRINYNN 872

BLAST of CmaCh02G010950 vs. ExPASy Swiss-Prot
Match: E9Q634 (Unconventional myosin-Ie OS=Mus musculus OX=10090 GN=Myo1e PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.6e-12
Identity = 55/186 (29.57%), Postives = 94/186 (50.54%), Query Frame = 0

Query: 47  EDQDLFMGIKVRRKDSLHNDYKGDYIDVPSNQFLMKILQKQGEESVLFADKVLKFTASGK 106
           E  DL +  K RR++S++ ++ GDYI +     L + + K+  E + FAD V K+    K
Sbjct: 721 EASDLLLNKKERRRNSINRNFIGDYIGMEERPELQQFVGKR--EKIDFADTVTKYDRRFK 780

Query: 107 MKRRILLITEFAIYIIDPE-----------TDTLKRRVALAAVDKICLSELSDNFYAIVI 166
             +R LL+T   +Y+I  E            + LKRR+ +  +  + LS + D+ + I+ 
Sbjct: 781 GVKRDLLLTPKCLYLIGREKVKQGPDKGVVKEVLKRRIEVERILSVSLSTMQDDIF-ILH 840

Query: 167 PTEYDILMASTRKTEIVTVLVDAFKSSSDYELEIVFSNRF----------VYSAGGELIK 212
             EYD L+ S  KTE +++L   ++  +  +L + FSN             +SAGG   +
Sbjct: 841 EQEYDSLLESVFKTEFLSLLAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGS--R 900

BLAST of CmaCh02G010950 vs. ExPASy Swiss-Prot
Match: Q12965 (Unconventional myosin-Ie OS=Homo sapiens OX=9606 GN=MYO1E PE=1 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 7.3e-12
Identity = 54/186 (29.03%), Postives = 95/186 (51.08%), Query Frame = 0

Query: 47  EDQDLFMGIKVRRKDSLHNDYKGDYIDVPSNQFLMKILQKQGEESVLFADKVLKFTASGK 106
           E  DL +  K RR++S++ ++ GDYI +  +  L + + K+  E + FAD V K+    K
Sbjct: 721 EASDLLLNKKERRRNSINRNFIGDYIGMEEHPELQQFVGKR--EKIDFADTVTKYDRRFK 780

Query: 107 MKRRILLITEFAIYIIDPE-----------TDTLKRRVALAAVDKICLSELSDNFYAIVI 166
             +R LL+T   +Y+I  E            + LKR++ +  +  + LS + D+ + I+ 
Sbjct: 781 GVKRDLLLTPKCLYLIGREKVKQGPDKGLVKEVLKRKIEIERILSVSLSTMQDDIF-ILH 840

Query: 167 PTEYDILMASTRKTEIVTVLVDAFKSSSDYELEIVFSNRF----------VYSAGGELIK 212
             EYD L+ S  KTE +++L   ++  +  +L + FSN             +SAGG   +
Sbjct: 841 EQEYDSLLESVFKTEFLSLLAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGS--R 900

BLAST of CmaCh02G010950 vs. TAIR 10
Match: AT5G53310.1 (myosin heavy chain-related )

HSP 1 Score: 228.4 bits (581), Expect = 5.7e-60
Identity = 110/172 (63.95%), Postives = 143/172 (83.14%), Query Frame = 0

Query: 48  DQDLFMGIKVRRKDSLHNDYKGDYIDVPSNQFLMKILQKQGEESVLFADKVLKFTASGKM 107
           D   F G KVRRK S + +++GDY+ V S   LM+IL+KQG+ S+LFADKVLKFT SGKM
Sbjct: 35  DHTPFAGGKVRRKASRYREHRGDYLHVSSRPGLMRILEKQGDTSILFADKVLKFTGSGKM 94

Query: 108 KRRILLITEFAIYIIDPETDTLKRRVALAAVDKICLSELSDNFYAIVIPTEYDILMASTR 167
           KRRI ++T+FAIY+IDPET+ + RR+AL++V+K+CLS+LSDNF+AI+IPTEYD+ MASTR
Sbjct: 95  KRRIFILTDFAIYLIDPETEAMTRRIALSSVEKMCLSKLSDNFFAIIIPTEYDLFMASTR 154

Query: 168 KTEIVTVLVDAFKSSSDYELEIVFSNRFVYSAGGELIKEVQFEEVEGGVKTR 220
           KTE+V V+VD  KS+SDYELE++ SNRF Y+A   L+KEV FEE EGG+KT+
Sbjct: 155 KTELVQVMVDVTKSASDYELEVLLSNRFEYNASASLVKEVSFEESEGGIKTK 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P197061.3e-1631.40Myosin heavy chain IB OS=Acanthamoeba castellanii OX=5755 GN=MIB PE=1 SV=2[more]
P340924.2e-1531.76Myosin IB heavy chain OS=Dictyostelium discoideum OX=44689 GN=myoB PE=1 SV=2[more]
P341093.5e-1433.11Myosin ID heavy chain OS=Dictyostelium discoideum OX=44689 GN=myoD PE=1 SV=2[more]
E9Q6345.6e-1229.57Unconventional myosin-Ie OS=Mus musculus OX=10090 GN=Myo1e PE=1 SV=1[more]
Q129657.3e-1229.03Unconventional myosin-Ie OS=Homo sapiens OX=9606 GN=MYO1E PE=1 SV=2[more]
Match NameE-valueIdentityDescription
AT5G53310.15.7e-6063.95myosin heavy chain-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010926Class I myosin tail homology domainPFAMPF06017Myosin_TH1coord: 49..213
e-value: 1.5E-38
score: 132.4
IPR010926Class I myosin tail homology domainPROSITEPS51757TH1coord: 56..225
score: 23.516832
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR34969OS01G0621700 PROTEINcoord: 11..223
NoneNo IPR availablePANTHERPTHR34969:SF4SUBFAMILY NOT NAMEDcoord: 11..223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G010950.1CmaCh02G010950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016459 myosin complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0003774 cytoskeletal motor activity