CmaCh02G010740 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G010740
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	Cma_Chr02: 6392968 .. 6395774 (-)
RNA-Seq Expression	CmaCh02G010740
Synteny	CmaCh02G010740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCTTATCAAGAAAATGAGTTGGCCACAACTGGTTGGTCTCTAAAAGCAATTTACAGATTCAAAAACTCTCAAGTGCGTCAGAAATTCCATCGCTCAACGGAATGTGGTTATTCAGAAGAAAAGGGCCCTCCGGGTTTTCGTCTTCTTCCACCGCCGAGGAAGTTACAAACGGAATCGATGGCACAGGACTCACCGCCATTGTTACAGGTCCGAATTTACTCTAGAACAGTTCTTGTTTATGTATTTTGAACTGGGTATCATCCTCCTTCGATTATACATCTGGGTTTATGCTAATAGGTTTCCATTAGAATCTTCTGGGGATTGTGCTTTCGTTATTGTTTATAACATCTGGGTGTTCTTCTTCAGGAGCGTCGAGTGGTATTGGCTCTGAAACTGCGCGTGTTCTTGCATTACGCGGAGTCCATGTTGTTATGGGTATTAGGAACTTAGAAGCTGGTAGAAATGTAAGAGAAACCATCATCAAGGAGAACCCCTCTGCAAAAATTGATGCCATGGAGTTGGATCTTAGCTCCATGGCTTCTGTGAGAAAATTTGCCTCAAATTATCAGTCAGCTGGGTTTCCACTTAATATCCTCATGTAAGAATTGATAGCCACTACAGTGACATACAGGACTATAAGTTAGCTATCTTTTTCATATCTGTTCTTTTCTGTGGTGGAAGTAATAATGCAGGAGTCATGGCTGCCCCTTTTGAACTTTCAAAAGATAACATAGAACTGCAATTTGCAACAAATCATTTAGGTATGTCGCTTATGTGATAGATTATTTCTTGTGTACTGAAAGCTTTTAATCTATATGAACTTGCAAGAAGATGTATATAGGATACTCGTGAAACCATTGACACAACAAGAGTTTACATGAACTTGTTATGGCCCAACATGATTGGGTGAGAAATTGAGGCTGTGGCCATGGGGAGATTAGGCAGTAGGCCTTGCTTAGCTTTAGAACTTTGAGTTTATTGCATTGATATATTGGCTGCTATAGGAACTTTTGTACCAATTTTCATTCTATGAAGTAGAATTGATGGATAGTTTCTTAGTCTACAATAACAGTAAGGAAGCTAACTTATTATACAGCTGATCATTAATGACCTTAATACTCGAGTTCGTGCAGGTCATTTCCTCTTGACAGATCTACTGTTGGATAGTATGAAGAAAACTGCCATTAAAAGTAAAAAAGAAGGAAGAATTGTTAACGTCTCCTCAGAAGCTCACCGTTATACATATCCTGAAGGCATCCGATTTGACGGAATTAACGACGAATCAAGGTAAGTGCTAGGCAGTTCTTTGTTTTCCTCCTACTGAAATGCAGATGACTTTCAACTCCTTACATTGCCTGCAGGATAGAAACAAACAAGTAACTCAGTTTGCATATTTGCTGCTTGCTGCCATTTTTTCCGTTTGCTTTGTTTTAAGTATTGCCCTCAAAGTGATCAAATTGCCACTTCCTTTCCAATTGATATTCAGAGTAGCTAGAAATTAATGAAAGATGAAATGTTTTTCTATGAACTTAAGCTTTGAGCTTTCTTGTTACATTTATTCATATAAAAGTTTGATAGTTCATGTGTTCAAATTCTCTCATTGTAGGTACAAAAAAATGCTAGCTTATGGCCAATCAAAGTTATGTAATGTGCTGCATGCCAATGAACTTACAAGACGTTTCAAGGTACGGGTACTTGTTTGGTTGATGGTGGAAAAAAACGAACCAAAAACGAGAAACGAGAAACAATGAACATATAAGGGCATAATATAATAAATTTAAGTTCAATCTGTGTCTTCCAAATCTAAAATTATTATTCATTGGCAAACTTCAGTCAATGCTGCTGTTACTCTTACTACAGTCTGATAGATATGTTCTTCATATAGGGAGGCAAATTATTGAGACCAAGAGCTTATTTCGTTAAATTTCGTTCATTTTCTTTTTCTTGTTTTGTTCACTAATTAAAACTGATGGGTTGTTCTTGTACAGGAAGAAGGAGTAAACATAACTGCAAACTCACTTCACCCAGGAATAATTGCCACAAATCTTTTCCGCCACTTCAATTTTGGCAATGGTAATACCGAAGCTCCGTTCGATAACAAATTAATAACAATGTGAATAGTATTTATAGGCTTACCAAACGATTATCAAACGGGTCTTTAGTTAACAGTTGCTTATACATTTGGGATCCTATTGAAGTCTATTTCTCACATCACATTGATGGAAGCTGACTTTTCTATTACTGCGGCGATGAAATTGGGCAGAAATCTCCTCTGTTCTGTTGTCTATCTTGAATGCTTACGTTTTCATATGAAAACTTCTCCCTACATGAGTATAATGCAGCGTTCAGTTCAGTAGATATGCATCTTAAAACTATAGACTTATAATTTTTCGAATCCACCAATTTTTTCTTCTTGTATTATTGCTAGCTTTATCAGCCTGAAAACTACTTTTGTTTTTCGTGTAGGTATTGCGAACACTGTCGGCAAACTCATATTTAAAAATGTTCAACAGGTAATCTTCATTCTAATGACGAAATCGTTATAAAACAAAACTTCTGCGTTCTTATGTAGAATATCTCGACCAATAGAAGCAAAATGCATATTGGGGTTAGTTGTTTATTGACCAGGGTCCGAATTTGCAGGGTGCAGCAACAACCTGCTATGTAGCATTGCATCCACAAGTGAAGGGAGTGAGTGGTGAATATTTTCTGAATAGTAACCTAAACAAGGCAAGCCAACATGGGCAGAATGTTGATCTGGCTAAGAAACTTTGGGATTTTACCACTAACTTACTCAAATAA

mRNA sequence

ATGGTTCTTATCAAGAAAATGAGTTGGCCACAACTGGTTGGTCTCTAAAAGCAATTTACAGATTCAAAAACTCTCAAGTGCGTCAGAAATTCCATCGCTCAACGGAATGTGGTTATTCAGAAGAAAAGGGCCCTCCGGGTTTTCGTCTTCTTCCACCGCCGAGGAAGTTACAAACGGAATCGATGGCACAGGACTCACCGCCATTGTTACAGGAGCGTCGAGTGGTATTGGCTCTGAAACTGCGCGTGTTCTTGCATTACGCGGAGTCCATGTTGTTATGGGTATTAGGAACTTAGAAGCTGGTAGAAATGTAAGAGAAACCATCATCAAGGAGAACCCCTCTGCAAAAATTGATGCCATGGAGTTGGATCTTAGCTCCATGGCTTCTGTGAGAAAATTTGCCTCAAATTATCAGTCAGCTGGGTTTCCACTTAATATCCTCATTAATAATGCAGGAGTCATGGCTGCCCCTTTTGAACTTTCAAAAGATAACATAGAACTGCAATTTGCAACAAATCATTTAGGTCATTTCCTCTTGACAGATCTACTGTTGGATAGTATGAAGAAAACTGCCATTAAAAGTAAAAAAGAAGGAAGAATTGTTAACGTCTCCTCAGAAGCTCACCGTTATACATATCCTGAAGGCATCCGATTTGACGGAATTAACGACGAATCAAGGTACAAAAAAATGCTAGCTTATGGCCAATCAAAGTTATGTAATGTGCTGCATGCCAATGAACTTACAAGACGTTTCAAGGAAGAAGGAGTAAACATAACTGCAAACTCACTTCACCCAGGAATAATTGCCACAAATCTTTTCCGCCACTTCAATTTTGGCAATGGTATTGCGAACACTGTCGGCAAACTCATATTTAAAAATGTTCAACAGGGTGCAGCAACAACCTGCTATGTAGCATTGCATCCACAAGTGAAGGGAGTGAGTGGTGAATATTTTCTGAATAGTAACCTAAACAAGGCAAGCCAACATGGGCAGAATGTTGATCTGGCTAAGAAACTTTGGGATTTTACCACTAACTTACTCAAATAA

Coding sequence (CDS)

ATGTGGTTATTCAGAAGAAAAGGGCCCTCCGGGTTTTCGTCTTCTTCCACCGCCGAGGAAGTTACAAACGGAATCGATGGCACAGGACTCACCGCCATTGTTACAGGAGCGTCGAGTGGTATTGGCTCTGAAACTGCGCGTGTTCTTGCATTACGCGGAGTCCATGTTGTTATGGGTATTAGGAACTTAGAAGCTGGTAGAAATGTAAGAGAAACCATCATCAAGGAGAACCCCTCTGCAAAAATTGATGCCATGGAGTTGGATCTTAGCTCCATGGCTTCTGTGAGAAAATTTGCCTCAAATTATCAGTCAGCTGGGTTTCCACTTAATATCCTCATTAATAATGCAGGAGTCATGGCTGCCCCTTTTGAACTTTCAAAAGATAACATAGAACTGCAATTTGCAACAAATCATTTAGGTCATTTCCTCTTGACAGATCTACTGTTGGATAGTATGAAGAAAACTGCCATTAAAAGTAAAAAAGAAGGAAGAATTGTTAACGTCTCCTCAGAAGCTCACCGTTATACATATCCTGAAGGCATCCGATTTGACGGAATTAACGACGAATCAAGGTACAAAAAAATGCTAGCTTATGGCCAATCAAAGTTATGTAATGTGCTGCATGCCAATGAACTTACAAGACGTTTCAAGGAAGAAGGAGTAAACATAACTGCAAACTCACTTCACCCAGGAATAATTGCCACAAATCTTTTCCGCCACTTCAATTTTGGCAATGGTATTGCGAACACTGTCGGCAAACTCATATTTAAAAATGTTCAACAGGGTGCAGCAACAACCTGCTATGTAGCATTGCATCCACAAGTGAAGGGAGTGAGTGGTGAATATTTTCTGAATAGTAACCTAAACAAGGCAAGCCAACATGGGCAGAATGTTGATCTGGCTAAGAAACTTTGGGATTTTACCACTAACTTACTCAAATAA

Protein sequence

MWLFRRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGIRNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMAAPFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEGIRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRHFNFGNGIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVDLAKKLWDFTTNLLK

Homology

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BLAST of CmaCh02G010740 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.7e-131
Identity = 231/314 (73.57%), Postives = 273/314 (86.94%), Query Frame = 0


Query: 1   MWLF-RRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMG 60
           MW F  +KG SGFS SSTAE+VT+GID TGLTAIVTGASSGIG+ET RVLALRG HV+MG
Sbjct: 1   MWPFSSKKGVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMG 60

Query: 61  IRNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVM 120
           +RN+ A ++V++TI+K+ PSAK+DA+ELDLSS+ SV+KFAS + S+G PLNILINNAG+M
Sbjct: 61  VRNMVAAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIM 120

Query: 121 AAPFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPE 180
           A PF+LSKDNIELQFATNH+GHFLLT+LLLD+MKKT  +SKKEGRIVNV+SEAHR+ YPE
Sbjct: 121 ACPFKLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPE 180

Query: 181 GIRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFR 240
           GIRFD IND+S Y    AYGQSKL NVLHAN+LT+  KE+GVNITANSLHPG I TNLFR
Sbjct: 181 GIRFDKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR 240

Query: 241 HFNFGNGIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVD 300
           H +  NG+ N +GKL+ KNVQQGAATTCYVALHPQVKGVSGEYF +SN+ K + HG++VD
Sbjct: 241 HNSAVNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVD 300

Query: 301 LAKKLWDFTTNLLK 314
           LAKKLWDF+ NL+K
Sbjct: 301 LAKKLWDFSINLVK 314

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BLAST of CmaCh02G010740 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.9e-122
Identity = 223/312 (71.47%), Postives = 259/312 (83.01%), Query Frame = 0


Query: 1   MWLFRRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGI 60
           MW F  KG SGFSS STAEEVT+G+DGTGLTAIVTGASSGIG ETARVL+LRGVHVVM +
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMA 120
           RN ++G  V+E I+K+ P AK+D MELDLSSM SVRKFAS Y+S G PLN+LINNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 APFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEG 180
            PF LSKDNIELQFATNHLGHFLLT LLLD+MK T+ +SK+EGRIVN+SSEAHR++YPEG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRH 240
           +RFD IND+S Y  M AYGQSKLCNVLHANELT++ KE+GVNITANSLHPG I TNL R+
Sbjct: 181 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 FNFGNGIA-NTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVD 300
           FN    +A   V K I K+V QGAATTCYVAL+PQV GVSGEYF +SN+ K     ++ +
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKKLWDFTTNL 312
           LAKK+WDF+T L
Sbjct: 301 LAKKVWDFSTKL 312

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BLAST of CmaCh02G010740 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.3e-96
Identity = 175/307 (57.00%), Postives = 225/307 (73.29%), Query Frame = 0


Query: 6   RKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGIRNLEA 65
           + G SG+ S+STAE+VT+ ID   LTAI+TG +SGIG E ARVL +RG HV++  RN +A
Sbjct: 10  KAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAARNTKA 69

Query: 66  GRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMAAPFEL 125
             + +E I++  P+A+ID ++LDLSS+ SVR F   + +   PLNILINNAGVM  PF+L
Sbjct: 70  ANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCPFQL 129

Query: 126 SKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEGIRFDG 185
           S+D IE QFATNH+GHFLLT+LLLD MK +A +S  EGRIVN+SS AH YTY EGI FD 
Sbjct: 130 SEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDY 189

Query: 186 INDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRHFNFGN 245
           IND  RY +  AYGQSKL N+LH+N L+R+ +EEGVNIT NS+HPG+I TNLFRH   G 
Sbjct: 190 INDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHSGLGM 249

Query: 246 GIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVDLAKKLW 305
            +   +   ++KN+ QGAATTCYVALHP +KGV+G+YF + N+   S    +  LA KLW
Sbjct: 250 AVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTLADKLW 309

Query: 306 DFTTNLL 313
           DF+  L+
Sbjct: 310 DFSIKLV 316

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BLAST of CmaCh02G010740 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.5e-95
Identity = 174/308 (56.49%), Postives = 225/308 (73.05%), Query Frame = 0


Query: 6   RKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGIRNLEA 65
           + G SG+ S+STAE+VT+ ID   LTAI+TG +SGIG E ARVL +RG HV++  RN +A
Sbjct: 10  KAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASRNTKA 69

Query: 66  GRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMAAPFEL 125
             + +E I++  P+A+ID ++LDLSS+ SVR F   + +   PLNILINNAGVM  PF+L
Sbjct: 70  ANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCPFQL 129

Query: 126 SKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEGIRFDG 185
           S+D IE QFATNH+GHFLLT+LLLD MK +A +S  EGRIVN+SS AH YTY EGI FD 
Sbjct: 130 SEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDY 189

Query: 186 INDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRHFNFGN 245
           IND  RY +  AYGQSKL N+LH+N L+R+ +EEGVNIT NS+HPG+I TNLFRH   G 
Sbjct: 190 INDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHSGLGM 249

Query: 246 GIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVDLAKKLW 305
            +   +   ++KN+ QGAATTCYVALHP +K V+G+YF + N+   S    +  LA KLW
Sbjct: 250 AVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTLADKLW 309

Query: 306 DFTTNLLK 314
           DF+  L++
Sbjct: 310 DFSIKLVE 317

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BLAST of CmaCh02G010740 vs. ExPASy Swiss-Prot
Match: Q91WL8 (WW domain-containing oxidoreductase OS=Mus musculus OX=10090 GN=Wwox PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.7e-52
Identity = 120/315 (38.10%), Postives = 180/315 (57.14%), Query Frame = 0


Query: 12  FSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGIRNLEAGRNVRE 71
           +  S+TA E+  G D TG   +VTGA+SGIG ETA+  AL G HV++  RNL        
Sbjct: 107 YDGSTTAMEILQGRDFTGKVVLVTGANSGIGFETAKSFALHGAHVILACRNLSRASEAVS 166

Query: 72  TIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMAAPFELSKDNIE 131
            I++E   AK++AM LDL+ + SV+ FA  +++    L++L+ NAG  A P+ L+KD +E
Sbjct: 167 RILEEWHKAKVEAMTLDLAVLRSVQHFAEAFKAKNVSLHVLVCNAGTFALPWGLTKDGLE 226

Query: 132 LQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEGIRFDGIND--- 191
             F  NHLGHF L  LL D + +++       R++ VSSE+HR+T         IND   
Sbjct: 227 TTFQVNHLGHFYLVQLLQDVLCRSS-----PARVIVVSSESHRFT--------DINDSSG 286

Query: 192 ----------ESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLF 251
                      S Y  MLAY +SKLCN+L +NEL RR    GV  T+N++HPG +  +  
Sbjct: 287 KLDLSRLSPPRSDYWAMLAYNRSKLCNILFSNELHRRLSPRGV--TSNAVHPGNMMYSAI 346

Query: 252 RHFNFGNGIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNV 311
              ++   +  T+ +   K++QQGAATT Y A+ P+++G+ G YF N      S+  Q+ 
Sbjct: 347 HRNSWVYKLLFTLARPFTKSMQQGAATTVYCAVAPELEGLGGMYFNNCCRCLPSEEAQSE 406

Query: 312 DLAKKLWDFTTNLLK 314
           + A+ LW+ +  L++
Sbjct: 407 ETARALWELSERLIQ 406

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BLAST of CmaCh02G010740 vs. TAIR 10
Match: AT4G23420.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 442.6 bits (1137), Expect = 2.7e-124
Identity = 224/312 (71.79%), Postives = 259/312 (83.01%), Query Frame = 0


Query: 1   MWLFRRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGI 60
           MW F  KG SGFSS STAEEVT+G+DGTGLTAIVTGASSGIG ETARVLALRGVHVVM +
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAV 60

Query: 61  RNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMA 120
           RN  AG  V+E I+K+ P AK+D MEL+LSSM SVRKFAS Y+SAG PLN+LINNAG+MA
Sbjct: 61  RNTGAGAKVKEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 121 APFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEG 180
            PF LSKDNIELQFATNHLGHFLLT LLLD+MK T+ +SK+EGRIVNVSSEAHRY+YPEG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEG 180

Query: 181 IRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRH 240
           +RFD INDES Y  + AYGQSKLCNVLHANEL ++ KE+GVNITANSLHPG I TNL+ +
Sbjct: 181 VRFDKINDESSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGY 240

Query: 241 FN-FGNGIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVD 300
           FN +  G    V K + K+V QGAATTCYVAL+PQV GV+GEYF +SN+ K  +  ++ +
Sbjct: 241 FNSYLAGAVGAVAKYMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTE 300

Query: 301 LAKKLWDFTTNL 312
           LAKKLWDF+T L
Sbjct: 301 LAKKLWDFSTKL 312

[Close]

BLAST of CmaCh02G010740 vs. TAIR 10
Match: AT4G23420.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 442.6 bits (1137), Expect = 2.7e-124
Identity = 224/312 (71.79%), Postives = 259/312 (83.01%), Query Frame = 0


Query: 1   MWLFRRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGI 60
           MW F  KG SGFSS STAEEVT+G+DGTGLTAIVTGASSGIG ETARVLALRGVHVVM +
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAV 60

Query: 61  RNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMA 120
           RN  AG  V+E I+K+ P AK+D MEL+LSSM SVRKFAS Y+SAG PLN+LINNAG+MA
Sbjct: 61  RNTGAGAKVKEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 121 APFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEG 180
            PF LSKDNIELQFATNHLGHFLLT LLLD+MK T+ +SK+EGRIVNVSSEAHRY+YPEG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEG 180

Query: 181 IRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRH 240
           +RFD INDES Y  + AYGQSKLCNVLHANEL ++ KE+GVNITANSLHPG I TNL+ +
Sbjct: 181 VRFDKINDESSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGY 240

Query: 241 FN-FGNGIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVD 300
           FN +  G    V K + K+V QGAATTCYVAL+PQV GV+GEYF +SN+ K  +  ++ +
Sbjct: 241 FNSYLAGAVGAVAKYMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTE 300

Query: 301 LAKKLWDFTTNL 312
           LAKKLWDF+T L
Sbjct: 301 LAKKLWDFSTKL 312

[Close]

BLAST of CmaCh02G010740 vs. TAIR 10
Match: AT4G23430.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 440.3 bits (1131), Expect = 1.3e-123
Identity = 223/312 (71.47%), Postives = 259/312 (83.01%), Query Frame = 0


Query: 1   MWLFRRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGI 60
           MW F  KG SGFSS STAEEVT+G+DGTGLTAIVTGASSGIG ETARVL+LRGVHVVM +
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMA 120
           RN ++G  V+E I+K+ P AK+D MELDLSSM SVRKFAS Y+S G PLN+LINNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 APFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEG 180
            PF LSKDNIELQFATNHLGHFLLT LLLD+MK T+ +SK+EGRIVN+SSEAHR++YPEG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRH 240
           +RFD IND+S Y  M AYGQSKLCNVLHANELT++ KE+GVNITANSLHPG I TNL R+
Sbjct: 181 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 FNFGNGIA-NTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVD 300
           FN    +A   V K I K+V QGAATTCYVAL+PQV GVSGEYF +SN+ K     ++ +
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKKLWDFTTNL 312
           LAKK+WDF+T L
Sbjct: 301 LAKKVWDFSTKL 312

[Close]

BLAST of CmaCh02G010740 vs. TAIR 10
Match: AT4G23430.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 2.1e-121
Identity = 222/312 (71.15%), Postives = 258/312 (82.69%), Query Frame = 0


Query: 1   MWLFRRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGI 60
           MW F  KG SGFSS STAEEVT+G+DGTGLTAIVTGASSGIG ETARVL+LRGVHVVM +
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMA 120
           RN ++G  V+E I+K+ P AK+D MELDLSSM SVRKFAS Y+S G PLN+LINNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 APFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEG 180
            PF LSKDNIELQFATNHLGHFLLT LLLD+MK T+ +SK+EGRIVN+SSEAHR++YPEG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRH 240
           +RFD IND+S    M AYGQSKLCNVLHANELT++ KE+GVNITANSLHPG I TNL R+
Sbjct: 181 VRFDKINDKS--SSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 FNFGNGIA-NTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVD 300
           FN    +A   V K I K+V QGAATTCYVAL+PQV GVSGEYF +SN+ K     ++ +
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKKLWDFTTNL 312
           LAKK+WDF+T L
Sbjct: 301 LAKKVWDFSTKL 310

[Close]

BLAST of CmaCh02G010740 vs. TAIR 10
Match: AT4G11410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 431.8 bits (1109), Expect = 4.7e-121
Identity = 216/311 (69.45%), Postives = 252/311 (81.03%), Query Frame = 0


Query: 1   MWLFRRKGPSGFSSSSTAEEVTNGIDGTGLTAIVTGASSGIGSETARVLALRGVHVVMGI 60
           MW F  KG SGFS+ STAEEVT+GIDGTGLTAIVTGASSGIG ET RVLALRGVHVVM +
Sbjct: 1   MWPFWWKGASGFSARSTAEEVTHGIDGTGLTAIVTGASSGIGEETTRVLALRGVHVVMAV 60

Query: 61  RNLEAGRNVRETIIKENPSAKIDAMELDLSSMASVRKFASNYQSAGFPLNILINNAGVMA 120
           RN ++G  VR+ I+KE P AKID M+LDLSSMASVR FAS YQS   PLN+LINNAG+MA
Sbjct: 61  RNTDSGNQVRDKILKEIPQAKIDVMKLDLSSMASVRSFASEYQSLDLPLNLLINNAGIMA 120

Query: 121 APFELSKDNIELQFATNHLGHFLLTDLLLDSMKKTAIKSKKEGRIVNVSSEAHRYTYPEG 180
            PF LS DNIELQFATNHLGHFLLT+LLL+ MKKTA +S +EGRIV VSSE HR+ Y EG
Sbjct: 121 CPFLLSSDNIELQFATNHLGHFLLTNLLLERMKKTASESNREGRIVIVSSEGHRFAYREG 180

Query: 181 IRFDGINDESRYKKMLAYGQSKLCNVLHANELTRRFKEEGVNITANSLHPGIIATNLFRH 240
           ++FD INDE+RY  + AYGQSKL N+LHA EL R FKE+GVNITANSLHPG I TNL R+
Sbjct: 181 VQFDKINDEARYNTLQAYGQSKLGNILHATELARLFKEQGVNITANSLHPGSIMTNLLRY 240

Query: 241 FNFGNGIANTVGKLIFKNVQQGAATTCYVALHPQVKGVSGEYFLNSNLNKASQHGQNVDL 300
            +F N I N VGK + K++ QGAATTCY ALHPQ KGVSGEY +++N++  +  G++ DL
Sbjct: 241 HSFINTIGNAVGKYVLKSIPQGAATTCYAALHPQAKGVSGEYLMDNNISDPNSQGKDKDL 300

Query: 301 AKKLWDFTTNL 312
           AKKLW+F+  L
Sbjct: 301 AKKLWEFSLRL 311

The following BLAST results are available for this feature:

BLAST of CmaCh02G010740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6RVV4	3.7e-131	73.57	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
A2RVM0	1.9e-122	71.47	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
A0A078ISJ6	2.3e-96	57.00	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078IS66	1.5e-95	56.49	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
Q91WL8	7.7e-52	38.10	WW domain-containing oxidoreductase OS=Mus musculus OX=10090 GN=Wwox PE=1 SV=1	[more]

BLAST of CmaCh02G010740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23420.1	2.7e-124	71.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G23420.2	2.7e-124	71.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G23430.2	1.3e-123	71.47	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G23430.1	2.1e-121	71.15	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G11410.1	4.7e-121	69.45	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 157..173 score: 32.82 coord: 31..48 score: 46.81 coord: 221..238 score: 32.88 coord: 107..118 score: 47.12
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 31..180 e-value: 8.1E-28 score: 97.2
None	No IPR available	GENE3D	3.40.50.720		coord: 22..313 e-value: 5.1E-79 score: 267.4
None	No IPR available	PANTHER	PTHR24320:SF230	NAD(P)-BINDING ROSSMANN-FOLD PROTEIN	coord: 2..313
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 2..313
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 29..305 e-value: 1.31515E-103 score: 302.221
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 29..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G010740.1	CmaCh02G010740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016630	protochlorophyllide reductase activity
molecular_function	GO:0016491	oxidoreductase activity