CmaCh02G009730 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G009730
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	PLAT domain-containing protein
Location	Cma_Chr02: 5815025 .. 5816739 (-)
RNA-Seq Expression	CmaCh02G009730
Synteny	CmaCh02G009730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCCAAATCACGTGACCATGATCCATCTTTTCTTCCCCAGCCATGACCTGTATATAAACCAATCCTCCCCATCTCCTTCTGCCCTAAATCTCTAAATCTCCACCGGAAGTTCTGAAAATGGCGCCTCGTTGTCTCTTCTTTTCCCTCATCTTCATCGCTCTTTTCCTCTCTGCATTGGCTCGCAACACTAAAGTAAGTTGCGTCTCTTTTAATACATTTCTTCCTTCTTGCTTGATCTTCGTACTTTCATCATCGGAGCCGTTTTGATGATCTTCACTCAATTTGTTATATTTACTCGTGATTACTTTTGACTGTGTCTGTTTTGATCTTGTTGATCGATCGCCTTTAGTAGTCGAGTTGTTGTAATGTTTGAGATTGATTATTCATATTTTCTGACAGTCGAACTGATTCACAGATTGTAGTTTACAAAATACAGGATAATGATCCGATTTATGCGCTGATGATTGTCGATTGTATTTTGCAATAACGAAGTGAATGATTGATTCACATTTAAGATCGGTTTTGAGTTATGACAGGGAAACTAGTTATAGAAATGCTCTTTAGAAAATGCATTCGATTTCTCAGTTATTGATGATAGTCGATTGTATGTGTAAGAAATAACTAAATGAATGTTTAACGTTTCAGATTGATTGAGTTACGTCAGTTCTACGAATTGCAGAACTCTGTTTAGAAAATGCAAGATCATGCTCCCATTTCTGCGTTGATGATAGTCTTTTGTATATGTAATGCCGAATTGGATGAATGATTCACATTTCAGTTCAATTATGACTTATGAGTCATGATATGTTAGTTAATTATTGAAATGTTGTATTAGGCAAGATAATGCTCCAATTTCTGCGTTTATGATTCACAATGGTCGATTTTATGTGGAATAACTTATTGAATGAATGACTTGATGCAAATTAGTTTTAAATTGATATAAATCGCCGTAATTTCTGAGCGGATTGTGGTCCGTGGAAAATGATGGGAGTGTTTTCTGGTGCAGGAAAAATGCGTGTACACATTCTACGTGAAGACAGGTTCAGTACTGAAGGGAGGAACGGACGCCATAATCAGTGTCACAATCAGCGACGCAAATGGCCAATCGGTGGAGATCGCAGATCTTGAATCTTGGGGGCTGATGGAAAAAAACCATGACTATTTCGAACGAGACAATGTCGACATATTCAGTGGGCGGGGCCCGTGCATCGAAGCGCCATTGTGTCGATTAAACCTGACCTCCGACGGATCAGGCGCCCACCACGGCTGGTTTTGCGACTATCTGGAGGTGACTTCGACTGGGCCACACAGATCCTGCTCCCAGACGGCGTTTTATGTGGACCAGTGGCTGGCCACCGACGCGCCGCCATTTCAGCTGACGACGATTCTGGACGGGTGCGATGATTGGATCAAGGGACATGAAGCGTCGAGGCATACGAGTAGAGGAAAGCTGGTGGTCTCCGGTTCCAAAGGATCTGCTTCGTTGTAACGGAATGCGATCGAAAAGTGAGTCGTATACGGATGTTTGGAAGTGATTTTACATCTTAATTTAAATTAAATAATGGGCATTTTCTTACGTCATTTCCCGAATTTCCACGTTTTTTCTTTTGGTTGTGAGATGTAAATTTCTTCGTCCGACTACGATTATATATTTTTTAGATCATACGTGACGTGTCAGCAAGTTATAATAAAATTTTTATGGTTTGGAATAAAAA

mRNA sequence

GTCCAAATCACGTGACCATGATCCATCTTTTCTTCCCCAGCCATGACCTGTATATAAACCAATCCTCCCCATCTCCTTCTGCCCTAAATCTCTAAATCTCCACCGGAAGTTCTGAAAATGGCGCCTCGTTGTCTCTTCTTTTCCCTCATCTTCATCGCTCTTTTCCTCTCTGCATTGGCTCGCAACACTAAAGAAAAATGCGTGTACACATTCTACGTGAAGACAGGTTCAGTACTGAAGGGAGGAACGGACGCCATAATCAGTGTCACAATCAGCGACGCAAATGGCCAATCGGTGGAGATCGCAGATCTTGAATCTTGGGGGCTGATGGAAAAAAACCATGACTATTTCGAACGAGACAATGTCGACATATTCAGTGGGCGGGGCCCGTGCATCGAAGCGCCATTGTGTCGATTAAACCTGACCTCCGACGGATCAGGCGCCCACCACGGCTGGTTTTGCGACTATCTGGAGGTGACTTCGACTGGGCCACACAGATCCTGCTCCCAGACGGCGTTTTATGTGGACCAGTGGCTGGCCACCGACGCGCCGCCATTTCAGCTGACGACGATTCTGGACGGGTGCGATGATTGGATCAAGGGACATGAAGCGTCGAGGCATACGAGTAGAGGAAAGCTGGTGGTCTCCGGTTCCAAAGGATCTGCTTCGTTGTAACGGAATGCGATCGAAAAGTGAGTCGTATACGGATGTTTGGAAGTGATTTTACATCTTAATTTAAATTAAATAATGGGCATTTTCTTACGTCATTTCCCGAATTTCCACGTTTTTTCTTTTGGTTGTGAGATGTAAATTTCTTCGTCCGACTACGATTATATATTTTTTAGATCATACGTGACGTGTCAGCAAGTTATAATAAAATTTTTATGGTTTGGAATAAAAA

Coding sequence (CDS)

ATGGCGCCTCGTTGTCTCTTCTTTTCCCTCATCTTCATCGCTCTTTTCCTCTCTGCATTGGCTCGCAACACTAAAGAAAAATGCGTGTACACATTCTACGTGAAGACAGGTTCAGTACTGAAGGGAGGAACGGACGCCATAATCAGTGTCACAATCAGCGACGCAAATGGCCAATCGGTGGAGATCGCAGATCTTGAATCTTGGGGGCTGATGGAAAAAAACCATGACTATTTCGAACGAGACAATGTCGACATATTCAGTGGGCGGGGCCCGTGCATCGAAGCGCCATTGTGTCGATTAAACCTGACCTCCGACGGATCAGGCGCCCACCACGGCTGGTTTTGCGACTATCTGGAGGTGACTTCGACTGGGCCACACAGATCCTGCTCCCAGACGGCGTTTTATGTGGACCAGTGGCTGGCCACCGACGCGCCGCCATTTCAGCTGACGACGATTCTGGACGGGTGCGATGATTGGATCAAGGGACATGAAGCGTCGAGGCATACGAGTAGAGGAAAGCTGGTGGTCTCCGGTTCCAAAGGATCTGCTTCGTTGTAA

Protein sequence

MAPRCLFFSLIFIALFLSALARNTKEKCVYTFYVKTGSVLKGGTDAIISVTISDANGQSVEIADLESWGLMEKNHDYFERDNVDIFSGRGPCIEAPLCRLNLTSDGSGAHHGWFCDYLEVTSTGPHRSCSQTAFYVDQWLATDAPPFQLTTILDGCDDWIKGHEASRHTSRGKLVVSGSKGSASL

Homology

[Close]

BLAST of CmaCh02G009730 vs. ExPASy Swiss-Prot
Match: O65660 (PLAT domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=PLAT1 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.7e-41
Identity = 77/158 (48.73%), Postives = 109/158 (68.99%), Query Frame = 0


Query: 1   MAPRCLFFSLIFIALFLSALA-RNTKEKCVYTFYVKTGSVLKGGTDAIISVTISDANGQS 60
           MA R +    + +   +SA+A       CVYTFY++TGS+ K GTD+IIS  I D +G  
Sbjct: 1   MARRDVLLPFLLLLATVSAVAFAEDDPDCVYTFYLRTGSIWKAGTDSIISARIYDKDGDY 60

Query: 61  VEIADLESW-GLMEKNHDYFERDNVDIFSGRGPCIEAPLCRLNLTSDGSGAHHGWFCDYL 120
           + I +L++W GLM  +++YFER N+DIFSGR PC+ +P+C LNLTSDGSG HHGW+ +Y+
Sbjct: 61  IGIKNLQAWAGLMGPDYNYFERGNLDIFSGRAPCLPSPICALNLTSDGSGDHHGWYVNYV 120

Query: 121 EVTSTGPHRSCSQTAFYVDQWLATDAPPFQLTTILDGC 157
           E+T+ G H  CS   F ++QWLATD  P++LT + + C
Sbjct: 121 EITTAGVHAQCSTQDFEIEQWLATDTSPYELTAVRNNC 158

[Close]

BLAST of CmaCh02G009730 vs. ExPASy Swiss-Prot
Match: Q9SIE7 (PLAT domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PLAT2 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.1e-40
Identity = 78/160 (48.75%), Postives = 112/160 (70.00%), Query Frame = 0


Query: 1   MAPR--CLFFSLIFIALFLSALA-RNTKEKCVYTFYVKTGSVLKGGTDAIISVTISDANG 60
           M PR   LF SL+ +   +SA+A  + +  CVYTF+++TGS  K GTD+IIS  + D  G
Sbjct: 1   MMPRRDVLFLSLLLVIATVSAVALADDEADCVYTFFLRTGSTFKAGTDSIISARVYDKYG 60

Query: 61  QSVEIADLESW-GLMEKNHDYFERDNVDIFSGRGPCIEAPLCRLNLTSDGSGAHHGWFCD 120
             + I +LE+W GLM   ++Y+ER N+DIFSG+ PC+ +P+C LNLTSDGSG HHGW+ +
Sbjct: 61  DYIGIRNLEAWGGLMGPGYNYYERGNLDIFSGKAPCLPSPVCSLNLTSDGSGDHHGWYVN 120

Query: 121 YLEVTSTGPHRSCSQTAFYVDQWLATDAPPFQLTTILDGC 157
           Y+EVT+ G H  CS  +F V+QWLA+D  P++L+ + + C
Sbjct: 121 YVEVTTAGVHAKCSYQSFDVEQWLASDTSPYELSAVRNNC 160

[Close]

BLAST of CmaCh02G009730 vs. ExPASy Swiss-Prot
Match: Q2V2V3 (PLAT domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLAT3 PE=4 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.9e-30
Identity = 61/104 (58.65%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 48  ISVTISDANGQSVEIADLESW-GLMEKNHDYFERDNVDIFSGRGPCIEAPLCRLNLTSDG 107
           +S+ + D+ GQ   I+DL SW GLM   HDYFER N+DIFSG G C+  P+C +NLTSDG
Sbjct: 1   MSLRLYDSYGQDAVISDLVSWGGLMGPFHDYFERGNLDIFSGLGSCLSGPVCAMNLTSDG 60

Query: 108 SGAHHGWFCDYLEVT-STGPHRSCSQTAFYVDQWLATDAPPFQL 150
           SG HHGW+C+Y+EVT S    RSCSQ  F V+QWLA DA P++L
Sbjct: 61  SGDHHGWYCNYVEVTMSESRRRSCSQEKFEVEQWLARDASPYEL 104

[Close]

BLAST of CmaCh02G009730 vs. ExPASy Swiss-Prot
Match: C8YR32 (Lipoxygenase homology domain-containing protein 1 OS=Mus musculus OX=10090 GN=Loxhd1 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.8e-07
Identity = 36/112 (32.14%), Postives = 50/112 (44.64%), Query Frame = 0


Query: 30   YTFYVKTGSVLKGGTDAIISVTISDANGQSVEIADLESWGLMEKNHDYFERDNVDIFSGR 89
            YT  VKT  +L  GTDA + + I   NG S  +A  +S      N + FER+N D F+  
Sbjct: 1682 YTVSVKTSDILGAGTDANVFIIIFGENGDSGTLALKQS-----ANWNKFERNNTDTFNFS 1741

Query: 90   GPCIEAPLCRLNLTSDGSGAHHGWFCDYLEVTSTGPHRSCSQTAFYVDQWLA 142
                   LC+L +  D  G   GW   Y++V       +     F  D WL+
Sbjct: 1742 DMLSLGHLCKLRVWHDNKGIFPGWHLSYVDVKDNSRDET---FRFQCDCWLS 1785

[Close]

BLAST of CmaCh02G009730 vs. ExPASy Swiss-Prot
Match: Q8IVV2 (Lipoxygenase homology domain-containing protein 1 OS=Homo sapiens OX=9606 GN=LOXHD1 PE=2 SV=4)

HSP 1 Score: 55.5 bits (132), Expect = 7.6e-07
Identity = 36/112 (32.14%), Postives = 50/112 (44.64%), Query Frame = 0


Query: 30   YTFYVKTGSVLKGGTDAIISVTISDANGQSVEIADLESWGLMEKNHDYFERDNVDIFSGR 89
            YT  VKT  +L  GTDA + + I   NG S  +A  +S      N + FER+N D F+  
Sbjct: 1681 YTVAVKTSDILGAGTDANVFIIIFGENGDSGTLALKQS-----ANWNKFERNNTDTFNFP 1740

Query: 90   GPCIEAPLCRLNLTSDGSGAHHGWFCDYLEVTSTGPHRSCSQTAFYVDQWLA 142
                   LC+L +  D  G   GW   Y++V       +     F  D WL+
Sbjct: 1741 DMLSLGHLCKLRVWHDNKGIFPGWHLSYVDVKDNSRDETFH---FQCDCWLS 1784

[Close]

BLAST of CmaCh02G009730 vs. TAIR 10
Match: AT4G39730.1 (Lipase/lipooxygenase, PLAT/LH2 family protein )

HSP 1 Score: 169.1 bits (427), Expect = 3.4e-42
Identity = 77/158 (48.73%), Postives = 109/158 (68.99%), Query Frame = 0


Query: 1   MAPRCLFFSLIFIALFLSALA-RNTKEKCVYTFYVKTGSVLKGGTDAIISVTISDANGQS 60
           MA R +    + +   +SA+A       CVYTFY++TGS+ K GTD+IIS  I D +G  
Sbjct: 1   MARRDVLLPFLLLLATVSAVAFAEDDPDCVYTFYLRTGSIWKAGTDSIISARIYDKDGDY 60

Query: 61  VEIADLESW-GLMEKNHDYFERDNVDIFSGRGPCIEAPLCRLNLTSDGSGAHHGWFCDYL 120
           + I +L++W GLM  +++YFER N+DIFSGR PC+ +P+C LNLTSDGSG HHGW+ +Y+
Sbjct: 61  IGIKNLQAWAGLMGPDYNYFERGNLDIFSGRAPCLPSPICALNLTSDGSGDHHGWYVNYV 120

Query: 121 EVTSTGPHRSCSQTAFYVDQWLATDAPPFQLTTILDGC 157
           E+T+ G H  CS   F ++QWLATD  P++LT + + C
Sbjct: 121 EITTAGVHAQCSTQDFEIEQWLATDTSPYELTAVRNNC 158

[Close]

BLAST of CmaCh02G009730 vs. TAIR 10
Match: AT2G22170.1 (Lipase/lipooxygenase, PLAT/LH2 family protein )

HSP 1 Score: 167.9 bits (424), Expect = 7.5e-42
Identity = 78/160 (48.75%), Postives = 112/160 (70.00%), Query Frame = 0


Query: 1   MAPR--CLFFSLIFIALFLSALA-RNTKEKCVYTFYVKTGSVLKGGTDAIISVTISDANG 60
           M PR   LF SL+ +   +SA+A  + +  CVYTF+++TGS  K GTD+IIS  + D  G
Sbjct: 1   MMPRRDVLFLSLLLVIATVSAVALADDEADCVYTFFLRTGSTFKAGTDSIISARVYDKYG 60

Query: 61  QSVEIADLESW-GLMEKNHDYFERDNVDIFSGRGPCIEAPLCRLNLTSDGSGAHHGWFCD 120
             + I +LE+W GLM   ++Y+ER N+DIFSG+ PC+ +P+C LNLTSDGSG HHGW+ +
Sbjct: 61  DYIGIRNLEAWGGLMGPGYNYYERGNLDIFSGKAPCLPSPVCSLNLTSDGSGDHHGWYVN 120

Query: 121 YLEVTSTGPHRSCSQTAFYVDQWLATDAPPFQLTTILDGC 157
           Y+EVT+ G H  CS  +F V+QWLA+D  P++L+ + + C
Sbjct: 121 YVEVTTAGVHAKCSYQSFDVEQWLASDTSPYELSAVRNNC 160

[Close]

BLAST of CmaCh02G009730 vs. TAIR 10
Match: AT5G65158.1 (Lipase/lipooxygenase, PLAT/LH2 family protein )

HSP 1 Score: 132.5 bits (332), Expect = 3.5e-31
Identity = 61/104 (58.65%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 48  ISVTISDANGQSVEIADLESW-GLMEKNHDYFERDNVDIFSGRGPCIEAPLCRLNLTSDG 107
           +S+ + D+ GQ   I+DL SW GLM   HDYFER N+DIFSG G C+  P+C +NLTSDG
Sbjct: 1   MSLRLYDSYGQDAVISDLVSWGGLMGPFHDYFERGNLDIFSGLGSCLSGPVCAMNLTSDG 60

Query: 108 SGAHHGWFCDYLEVT-STGPHRSCSQTAFYVDQWLATDAPPFQL 150
           SG HHGW+C+Y+EVT S    RSCSQ  F V+QWLA DA P++L
Sbjct: 61  SGDHHGWYCNYVEVTMSESRRRSCSQEKFEVEQWLARDASPYEL 104

The following BLAST results are available for this feature:

BLAST of CmaCh02G009730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65660	4.7e-41	48.73	PLAT domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=PLAT1 PE=1 S...	[more]
Q9SIE7	1.1e-40	48.75	PLAT domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PLAT2 PE=2 S...	[more]
Q2V2V3	4.9e-30	58.65	PLAT domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLAT3 PE=4 S...	[more]
C8YR32	5.8e-07	32.14	Lipoxygenase homology domain-containing protein 1 OS=Mus musculus OX=10090 GN=Lo...	[more]
Q8IVV2	7.6e-07	32.14	Lipoxygenase homology domain-containing protein 1 OS=Homo sapiens OX=9606 GN=LOX...	[more]

BLAST of CmaCh02G009730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39730.1	3.4e-42	48.73	Lipase/lipooxygenase, PLAT/LH2 family protein	[more]
AT2G22170.1	7.5e-42	48.75	Lipase/lipooxygenase, PLAT/LH2 family protein	[more]
AT5G65158.1	3.5e-31	58.65	Lipase/lipooxygenase, PLAT/LH2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001024	PLAT/LH2 domain	PFAM	PF01477	PLAT	coord: 30..144 e-value: 1.3E-11 score: 45.1
IPR001024	PLAT/LH2 domain	PROSITE	PS50095	PLAT	coord: 28..154 score: 21.928806
None	No IPR available	GENE3D	2.60.60.20	PLAT/LH2 domain	coord: 16..145 e-value: 2.6E-20 score: 74.5
None	No IPR available	PANTHER	PTHR31718:SF47	PLAT DOMAIN-CONTAINING PROTEIN 3-LIKE	coord: 7..182
None	No IPR available	PANTHER	PTHR31718	FAMILY NOT NAMED	coord: 7..182
IPR036392	PLAT/LH2 domain superfamily	SUPERFAMILY	49723	Lipase/lipooxygenase domain (PLAT/LH2 domain)	coord: 25..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G009730.1	CmaCh02G009730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005515	protein binding