CmaCh02G009420 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh02G009420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCma_Chr02: 5585122 .. 5588530 (+)
RNA-Seq ExpressionCmaCh02G009420
SyntenyCmaCh02G009420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGATTTCATCGGAGAGAATCATTCTCCCCCATCCGTCGCTGGAGGTCGTCGGCGGAGGTCGCGATCGGTTCCTCCCGGCTTTCAAGTACTTGGACAAACCCTATAAGAAATTCCCTGTAATTGGGTCCAATCGCCATTTGGAGACTATTTTTGCTTCCTTCTTTAGGACTTCCCCCAAGATTAAGCTCCGCCGCGAGTGCTTGCGTGTGGCGGATAATGGCACCATTGCTTTGGATTGGGTCGCCGGTGATCATCTCCGCCTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGGTTTGCGCCTTCTGATTCTTTGTTTTCTGAAAATCTTTTGAATTAGATATGATACAAAAGATGCTCGTATACCAAATGATGTTAAATTTGGAATTAGGAAGGGAATGATTATGTTAACCCCAACTCATAAACACTAGAACACTTGTTTAATTTAACATGGGGTCAAAGAATCTCATGTATCTATTTGAATGCTAATGGCATACTACCCAATTCTCGAATTGGGCATCCTTGAAGTGAAACCTTAAAGGCACTAAATTCGTTACTGCATTTAATTCTAATCTGCGTGTCTTGGATTTTGATTAAATGTTGAAATTGAACTTGCAGTTTGATCCTTGAATGGGGTTGATTTCTTGAAGCAAATGTGGCTCCTGTCAGAGTATTAAGGAAATAGTAGGAATCTTATTAGGATATTAGTAGTAGCATAGTAATTACTTTATAGTCTGTTCAAAGGGTGCTTATATACAGAGAGAGTTTGAGTGTGTATGAATTAGGATGATTTTTGGGAAATCAGTCCACGCAGGCAGATTGTGGGCTCGAGATAACCTTCTCGAAAGACGATTCGTACCTTAATTTCTCTTAGATATTACTATATAGTTACATTGTAGTTTTTTTGTACTTTTGATTCTAACACTTCCTGACTCATATCAATGGATTTCATATTAGTTTCTTGAATCTTTGCAGCCTGGTTTAACTGGAGGGAGTCAAGATTCATATGTAAGACATATGTTGGTTAGAGCCAGAGATCGTAGTTGGAGGGTTGTGGTCTTCAACAACCGCGGATGTGGGAATAGTCCTGTTACCTCTCCCCAGGTTCTTTATTCTTTTCCATGTTGGAGAATTGAAGCACATTGTATATAAGATGATTATTTTGTTTTTACAATACTCAGAGTCGACATTCCTGTTTATGATATATTTTGCAACACTGATACATGCAGTTCTATTCAGCTTCATTTTTGGGAGACATGCGTGATGTAGTTGCACATGTTACTACACGATACCCCGAGGCTAATGTGTATGCTGTTGGCTGGTCTCTTGGTGGTAACATTCTTGTAAATTATTTGGGACAGGTAAAACTAAAACATTACTTATTAATCTCCATCCTTCTTTTTATTCTACTTGTTGGTTACCATTCTTTCTAAAGCTTCCTAAAATCTCATAGAATGAGGGAAACTCGGAATTGAAATCGTGAGAAAAAGACCTTTAGAATGGTATCGTTGTGATGGGATAAGTAAAACAAAAAACAAAACTTCGGAGACCGAATGTTCAAAATGGACAATATTATATTATTTTGGAGGACCATCGTTTTAATAGGGCTCATGTCTACAAATCTTGTTGGGATGTGGACAACTAAACATGTAACGGGCCAAGCCCACCGCTAGCAGATATTGTCCTCTTTGAACTTTCTCTTTCAAGTTTCTCCTTAAAATGCGTCTGTTAGGGAGAGGTTTCCACAACTTTATAAAGGGTGTTTCGTTCTCATCTCCAACCGATGTGGGATCTCATTGGACATTTGTGAACGGTGCCTTCTTCACACCCCTTTACCAGCCTTCTATCATAAAACGTAAAAGCATTTCAACTAGGATATTGATTGTGTTTCAAGATATGTTGTAGTACAACTTGAAAAAAACAAAGCTGTTCTTTGTTGAATAACGTATTTGCCAATAATGCAAAGTTGAATTCCGAGAGTTTGGTGATCTGGGGATCCACTACTAACGAAAAGAACAAAATCTCTGTGTAGATTGTTTAAGTTTCACATGCATCTAGTAATTATTCTGATGTTGTTCATCTTTGTCTCATAAAATTCATTTGTACTCATTCATTTTCATTGCAGGATTGTATGTCCTGTCCATTGTCTGGTGCTGTGTCGTTGTGTAATCCTTTCGATTTAGTAGATACAGATGAGAACTTCCGGAAGGGCTTCAACAATGTTTATGACAAAGCCCTTGCTCGATCTTTATGTCGAAATTTCCAGAAGTAATTCTCTTTTGTGCAAATGTCTTTGTTCTGTTAACCATGTACCTAATACTGAAAAATTATTAGATACTTAGATTGTATCATCTGACTTGATTCCCCTGAGATTGCAGGCATGCTGATCTCTTCAATGATATGCCTGGTGAATTCAATCTCCCATTGGCTGCCAATGCATGGACTATTAGAGACTACGATGATGCCTTAACTCGCGGTATAATTTCTCTTAATTTTCGGTAGTTTCAATTGACATAAGTGTCGGTACACGTCGTTTCTTCTGCTTAGAGTTTTTCCTTTTTCTTCGAACAATTTTCAGTTTCATTTGGTTTCAACTCTGTGGATGAATATTATTCAAAGTCTAGCAGTTCAAAAGTCATAAAAGATGTCAGAATTCCATTGCTTTGCATCCAGGTACCTTTTTTTTTTTTGTTACTAAGGGTTGTCTTGTCTCACTGATCTCTAAGAACACAAGACTGCTGTTGTTTCTTTGCAGGCAGCAAATGATCCAATTGCTCCAGATAAAGCAATCCCCCGTGAGGATATAAAGGTTCGCATGTGCTTTTAGGCTTGATTTGATAACTATTTAACGGATATCATATGATATCATCATAGTGGTAGATTTTATGTTCATATCCCAGGAGTAGTTTAATATGTATCTCGTTGAGTTTAGCATACGTGTTTGTGTAATGTGATTGTTGGTGCAGGAAAATCCGAACTGTTTGTTGATAGTAACGCCCAAAGGTGGGCATCTAGGGTGGGTTGCAGGCTCTAGTGCTCCCCTGGGAGCTCCGTGGCCCGATCCTTTCGTGATCGATTTCCTCGTGCATTTGGAAAGCCAAGCATCCAAAGCTTCTGCAGCAGAGGGGTTGGCATAACCATTGATGGGTATAAGAATTGCAAATAGAGCTATGATTTGTGAAATGATTAAACTCAATGCCATGTCTAGATCATTTAAAATTGGTAATTGTAAATTTATTTTAAATAAAGAAATTATGGTCCAATAAGAATTGGTTAGAATCAATTACCCCTTTAGTATCTCTAGCTAGTTCTTCTTCCCAAAGTGTTGTGTTGGTAGTGTTCTGCAACACTTCTTGAACATCCCTTTTCATTTGAAACCTTCATATCTATTCAAGTTCCTTCC

mRNA sequence

ATGGCGGAGATTTCATCGGAGAGAATCATTCTCCCCCATCCGTCGCTGGAGGTCGTCGGCGGAGGTCGCGATCGGTTCCTCCCGGCTTTCAAGTACTTGGACAAACCCTATAAGAAATTCCCTGTAATTGGGTCCAATCGCCATTTGGAGACTATTTTTGCTTCCTTCTTTAGGACTTCCCCCAAGATTAAGCTCCGCCGCGAGTGCTTGCGTGTGGCGGATAATGGCACCATTGCTTTGGATTGGGTCGCCGGTGATCATCTCCGCCTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACTGGAGGGAGTCAAGATTCATATGTAAGACATATGTTGGTTAGAGCCAGAGATCGTAGTTGGAGGGTTGTGGTCTTCAACAACCGCGGATGTGGGAATAGTCCTGTTACCTCTCCCCAGTTCTATTCAGCTTCATTTTTGGGAGACATGCGTGATGTAGTTGCACATGTTACTACACGATACCCCGAGGCTAATGTGTATGCTGTTGGCTGGTCTCTTGGTGGTAACATTCTTGTAAATTATTTGGGACAGGATTGTATGTCCTGTCCATTGTCTGGTGCTGTGTCGTTGTGTAATCCTTTCGATTTAGTAGATACAGATGAGAACTTCCGGAAGGGCTTCAACAATGTTTATGACAAAGCCCTTGCTCGATCTTTATGTCGAAATTTCCAGAAGCATGCTGATCTCTTCAATGATATGCCTGGTGAATTCAATCTCCCATTGGCTGCCAATGCATGGACTATTAGAGACTACGATGATGCCTTAACTCGCGTTTCATTTGGTTTCAACTCTGTGGATGAATATTATTCAAAGTCTAGCAGTTCAAAAGTCATAAAAGATGTCAGAATTCCATTGCTTTGCATCCAGGCAGCAAATGATCCAATTGCTCCAGATAAAGCAATCCCCCGTGAGGATATAAAGGAAAATCCGAACTGTTTGTTGATAGTAACGCCCAAAGGTGGGCATCTAGGGTGGGTTGCAGGCTCTAGTGCTCCCCTGGGAGCTCCGTGGCCCGATCCTTTCGTGATCGATTTCCTCGTGCATTTGGAAAGCCAAGCATCCAAAGCTTCTGCAGCAGAGGGGTTGGCATAACCATTGATGGGTATAAGAATTGCAAATAGAGCTATGATTTGTGAAATGATTAAACTCAATGCCATGTCTAGATCATTTAAAATTGGTAATTGTAAATTTATTTTAAATAAAGAAATTATGGTCCAATAAGAATTGGTTAGAATCAATTACCCCTTTAGTATCTCTAGCTAGTTCTTCTTCCCAAAGTGTTGTGTTGGTAGTGTTCTGCAACACTTCTTGAACATCCCTTTTCATTTGAAACCTTCATATCTATTCAAGTTCCTTCC

Coding sequence (CDS)

ATGGCGGAGATTTCATCGGAGAGAATCATTCTCCCCCATCCGTCGCTGGAGGTCGTCGGCGGAGGTCGCGATCGGTTCCTCCCGGCTTTCAAGTACTTGGACAAACCCTATAAGAAATTCCCTGTAATTGGGTCCAATCGCCATTTGGAGACTATTTTTGCTTCCTTCTTTAGGACTTCCCCCAAGATTAAGCTCCGCCGCGAGTGCTTGCGTGTGGCGGATAATGGCACCATTGCTTTGGATTGGGTCGCCGGTGATCATCTCCGCCTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACTGGAGGGAGTCAAGATTCATATGTAAGACATATGTTGGTTAGAGCCAGAGATCGTAGTTGGAGGGTTGTGGTCTTCAACAACCGCGGATGTGGGAATAGTCCTGTTACCTCTCCCCAGTTCTATTCAGCTTCATTTTTGGGAGACATGCGTGATGTAGTTGCACATGTTACTACACGATACCCCGAGGCTAATGTGTATGCTGTTGGCTGGTCTCTTGGTGGTAACATTCTTGTAAATTATTTGGGACAGGATTGTATGTCCTGTCCATTGTCTGGTGCTGTGTCGTTGTGTAATCCTTTCGATTTAGTAGATACAGATGAGAACTTCCGGAAGGGCTTCAACAATGTTTATGACAAAGCCCTTGCTCGATCTTTATGTCGAAATTTCCAGAAGCATGCTGATCTCTTCAATGATATGCCTGGTGAATTCAATCTCCCATTGGCTGCCAATGCATGGACTATTAGAGACTACGATGATGCCTTAACTCGCGTTTCATTTGGTTTCAACTCTGTGGATGAATATTATTCAAAGTCTAGCAGTTCAAAAGTCATAAAAGATGTCAGAATTCCATTGCTTTGCATCCAGGCAGCAAATGATCCAATTGCTCCAGATAAAGCAATCCCCCGTGAGGATATAAAGGAAAATCCGAACTGTTTGTTGATAGTAACGCCCAAAGGTGGGCATCTAGGGTGGGTTGCAGGCTCTAGTGCTCCCCTGGGAGCTCCGTGGCCCGATCCTTTCGTGATCGATTTCCTCGTGCATTTGGAAAGCCAAGCATCCAAAGCTTCTGCAGCAGAGGGGTTGGCATAA

Protein sequence

MAEISSERIILPHPSLEVVGGGRDRFLPAFKYLDKPYKKFPVIGSNRHLETIFASFFRTSPKIKLRRECLRVADNGTIALDWVAGDHLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRSWRVVVFNNRGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPEANVYAVGWSLGGNILVNYLGQDCMSCPLSGAVSLCNPFDLVDTDENFRKGFNNVYDKALARSLCRNFQKHADLFNDMPGEFNLPLAANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSSKVIKDVRIPLLCIQAANDPIAPDKAIPREDIKENPNCLLIVTPKGGHLGWVAGSSAPLGAPWPDPFVIDFLVHLESQASKASAAEGLA
Homology
BLAST of CmaCh02G009420 vs. ExPASy Swiss-Prot
Match: Q40863 (Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.5e-126
Identity = 221/352 (62.78%), Postives = 264/352 (75.00%), Query Frame = 0

Query: 17  EVVGGGRDRFLPAFKYLDKPYKKFPVIGSNRHLETIFASFFRTSPKIKLRRECLRVADNG 76
           E++G  R+  +     L +PY+ FP +G NRH+ETIFASFFR+ P IK RRECLR+ D G
Sbjct: 69  EIIGSDRE-LMSKLTTLGRPYRHFPFMG-NRHVETIFASFFRSWPVIKSRRECLRMEDGG 128

Query: 77  TIALDW-VAGDHL-----RLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRSWRVVVFN 136
           T+ LDW + G+        LP++SPVLILLPGLTGGS DSYV+HML+RAR   W  VVFN
Sbjct: 129 TVELDWPLEGEDAELWNGELPVNSPVLILLPGLTGGSDDSYVKHMLLRARKHGWHSVVFN 188

Query: 137 NRGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPEANVYAVGWSLGGNILVNYLGQDCM 196
           +RGC +SPVT+PQFYSASF  D+  VV HV  R+ E+N+YAVGWSLG NILV YLG+   
Sbjct: 189 SRGCADSPVTTPQFYSASFTKDLCQVVKHVAVRFSESNIYAVGWSLGANILVRYLGEVAG 248

Query: 197 SCPLSGAVSLCNPFDLVDTDENFRK--GFNNVYDKALARSLCRNFQKHADLFNDMPGEFN 256
           +CPLSGAVSLCNPF+LV  DE+F K  GFNNVYDKALAR L + F KH  LF  + GE+N
Sbjct: 249 NCPLSGAVSLCNPFNLVIADEDFHKGLGFNNVYDKALARGLRQIFPKHTRLFEGIEGEYN 308

Query: 257 LPLAANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSSKVIKDVRIPLLCIQAANDPIAPD 316
           +P  A A ++RD+D  LTRVSFGF SV +YYS SSSS  IK V+  LLCIQA+NDPIAP 
Sbjct: 309 IPTVAKARSVRDFDGGLTRVSFGFQSVGDYYSNSSSSLSIKYVQTSLLCIQASNDPIAPS 368

Query: 317 KAIPREDIKENPNCLLIVTPKGGHLGWVAGSSAPLGAPWPDPFVIDFLVHLE 361
           + IP EDIKENPNCLL+VTP GGHLGWVAG  AP GAPW DP V+++L  LE
Sbjct: 369 RGIPWEDIKENPNCLLVVTPNGGHLGWVAGDDAPFGAPWTDPLVMEYLEVLE 418

BLAST of CmaCh02G009420 vs. ExPASy Swiss-Prot
Match: Q0VC00 (Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.3e-50
Identity = 101/293 (34.47%), Postives = 167/293 (57.00%), Query Frame = 0

Query: 50  ETIFASFFRTSPKIKLRRECLRVADNGTIALDWVAGDHLRLPLDS---PVLILLPGLTGG 109
           +T+   F  + P ++ R E ++ AD G I+LDW   D+ +  +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPLVQYRNELIKTADGGQISLDWFDNDNSKHYMDASTRPTVLLLPGLTGT 151

Query: 110 SQDSYVRHMLVRARDRSWRVVVFNNRGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPE 169
           S++SY+ HM+  + +  +R VVFNNRG     + +P+ Y  S   D+  V+ HV + YP 
Sbjct: 152 SKESYILHMIHLSEELGYRYVVFNNRGVAGENLLTPRTYCCSNTEDLETVIHHVHSLYPS 211

Query: 170 ANVYAVGWSLGGNILVNYLGQDCMSCPLSGAVSLCNPFDLVDTDENFRKGFN-NVYDKAL 229
           A   A G S+GG +L+NYLG+     PL  A +    ++     E+  K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGPKTPLKAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 230 ARSLCRNFQKHADLFNDMPGEFNLPLAANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSS 289
              L  +  KH  +F     + ++     A +IR++D   T V FG+ ++D+YY+ +S +
Sbjct: 272 TTCLQSSVNKHRHMF---VKQIDVDHVMKAKSIREFDKRFTSVMFGYRTIDDYYTDASPN 331

Query: 290 KVIKDVRIPLLCIQAANDPIAPDKAIPREDIKENPNCLLIVTPKGGHLGWVAG 339
           + +K V IP+LC+ + +D  +P  AIP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of CmaCh02G009420 vs. ExPASy Swiss-Prot
Match: Q8WU67 (Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 2.3e-50
Identity = 101/293 (34.47%), Postives = 165/293 (56.31%), Query Frame = 0

Query: 50  ETIFASFFRTSPKIKLRRECLRVADNGTIALDWVAGDHLRLPLDS---PVLILLPGLTGG 109
           +T+   F  + P ++ R E ++ AD G I+LDW   D+    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNDNSTCYMDASTRPTILLLPGLTGT 151

Query: 110 SQDSYVRHMLVRARDRSWRVVVFNNRGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPE 169
           S++SY+ HM+  + +  +R VVFNNRG     + +P+ Y  +   D+  V+ HV + YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGENLLTPRTYCCANTEDLETVIHHVHSLYPS 211

Query: 170 ANVYAVGWSLGGNILVNYLGQDCMSCPLSGAVSLCNPFDLVDTDENFRKGFN-NVYDKAL 229
           A   A G S+GG +L+NYLG+     PL  A +    ++     E+  K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 230 ARSLCRNFQKHADLFNDMPGEFNLPLAANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSS 289
              L  +  KH  +F     + ++     A +IR++D   T V FG+ ++D+YY+ +S S
Sbjct: 272 TTCLQSSVNKHRHMF---VKQVDMDHVMKAKSIREFDKRFTSVMFGYQTIDDYYTDASPS 331

Query: 290 KVIKDVRIPLLCIQAANDPIAPDKAIPREDIKENPNCLLIVTPKGGHLGWVAG 339
             +K V IP+LC+ + +D  +P  AIP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 PRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of CmaCh02G009420 vs. ExPASy Swiss-Prot
Match: Q91ZH7 (Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.6e-50
Identity = 100/293 (34.13%), Postives = 168/293 (57.34%), Query Frame = 0

Query: 50  ETIFASFFRTSPKIKLRRECLRVADNGTIALDWVAGDHLRLPLDS---PVLILLPGLTGG 109
           +T+   F  + P ++ R E ++ AD G I+LDW   ++    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNNNSAYYVDASTRPTILLLPGLTGT 151

Query: 110 SQDSYVRHMLVRARDRSWRVVVFNNRGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPE 169
           S++SY+ HM+  + +  +R VVFNNRG     + +P+ Y  +   D+  VV HV + YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGESLLTPRTYCCANTEDLEAVVHHVHSLYPG 211

Query: 170 ANVYAVGWSLGGNILVNYLGQDCMSCPLSGAVSLCNPFDLVDTDENFRKGFN-NVYDKAL 229
           A   A G S+GG +L+NYLG+     PL  A +    ++     E+  +  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLERPLNWLLFNYYL 271

Query: 230 ARSLCRNFQKHADLFNDMPGEFNLPLAANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSS 289
              L  + +KH  +F +   + ++     A +IR++D   T V FG+ ++D+YY+ +S +
Sbjct: 272 TTCLQSSVKKHRHMFVE---QIDMDQVMKAKSIREFDKRFTAVMFGYRTLDDYYTDASPN 331

Query: 290 KVIKDVRIPLLCIQAANDPIAPDKAIPREDIKENPNCLLIVTPKGGHLGWVAG 339
           + +K V IP+LC+ A +D  +P  AIP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNATDDVFSPSHAIPIETAKQNPNVALVLTAYGGHIGFLEG 381

BLAST of CmaCh02G009420 vs. ExPASy Swiss-Prot
Match: Q54H38 (Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=abhd PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.8e-47
Identity = 113/317 (35.65%), Postives = 173/317 (54.57%), Query Frame = 0

Query: 46  NRHLETIFASFFRTSPKIKLRRECL-RVADNGTIALDWVAGDHLRLPLDSPVLILLPGLT 105
           N H    + S+      +K RRE L    D GTI+LD+      +   D+P +++  GLT
Sbjct: 69  NSHFMNYYGSYKIPKLNLKTRREILVNPIDGGTISLDFFELGEFK--EDTPTIVINHGLT 128

Query: 106 GGSQDSYVRHMLVRA-RDRSWRVVVFNNRGCGNSPVTSPQFYSASFLGDMRDVVAHVT-T 165
           GGS + YV++   RA +++ +R VVFN RGC  +P+T+ + YSA  L D++ V  ++T T
Sbjct: 129 GGSHERYVQYFAQRAYKEKGFRSVVFNYRGCAGNPITADRAYSAVQLDDIKFVTEYLTKT 188

Query: 166 RYPEANV-YAVGWSLGGNILVNYLGQDCMSCPLSGAVSLCNPFDLVDTDENFRKGF--NN 225
             P     + VG+SLG  ILVNY+       P    VS+ NP ++V+  +N    +  N 
Sbjct: 189 ALPLVKKWFLVGFSLGSAILVNYMADAGKDSPYLAHVSISNPMNMVECTKNLSSTYINNL 248

Query: 226 VYDKALARSLCRNFQKHADLFNDMPGEFNLPLAANAWTIRDYDDALTRVSFGFNSVDEYY 285
           +Y+K LA +L R F+K     +    +  +  A    TI D+DD +T   FGF +  +YY
Sbjct: 249 IYNKGLANNLKRLFRKFDGRLDKYATKEQIMAAQ---TIADFDDLITSKMFGFETAHDYY 308

Query: 286 SKSSSSKVIKDVRIPLLCIQAANDPIAPDKAIPREDIKENPNCLLIVTPKGGHLGWVAGS 345
             +SSSK I+++  P+L I A +DPIAP    P +D K NPN +L V+  GGHLG+++  
Sbjct: 309 LAASSSKSIRNLVKPILFINAIDDPIAPTSGFPWKDFKSNPNTILCVSRWGGHLGFISYE 368

Query: 346 SAPLGAPWPDPFVIDFL 357
                  W D   +++L
Sbjct: 369 D---HMSWSDKAAVEYL 377

BLAST of CmaCh02G009420 vs. TAIR 10
Match: AT3G50790.1 (esterase/lipase/thioesterase family protein )

HSP 1 Score: 550.4 bits (1417), Expect = 1.1e-156
Identity = 253/351 (72.08%), Postives = 299/351 (85.19%), Query Frame = 0

Query: 13  HPSLEVVGGGRDRFLPAFK-YLDKPYKKFPVIGSNRHLETIFASFFRTSPKIKLRRECLR 72
           H SLEV+GGG DRFLP+ K  L KPY  FP+IG NRH+ETI+ASFFR+ P ++LRRECLR
Sbjct: 40  HSSLEVIGGGSDRFLPSLKDSLAKPYNAFPLIGFNRHVETIYASFFRSVPFVRLRRECLR 99

Query: 73  VADNGTIALDWVAGDHLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRSWRVVVFNN 132
             DNG++ALDWVAG+    P DSP+LILLPGLTGGSQDSYVRHML+RA+ + WR VVFN+
Sbjct: 100 TKDNGSVALDWVAGEDRHFPPDSPILILLPGLTGGSQDSYVRHMLLRAQSKKWRCVVFNS 159

Query: 133 RGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPEANVYAVGWSLGGNILVNYLGQDCMS 192
           RGCG+SPVT+PQFYSASFLGD+ +V+ HV  ++P+AN+YA GWSLGGNILVNYLGQ+  +
Sbjct: 160 RGCGDSPVTTPQFYSASFLGDIGEVIDHVVDKFPKANLYAAGWSLGGNILVNYLGQESHN 219

Query: 193 CPLSGAVSLCNPFDLVDTDENFRKGFNNVYDKALARSLCRNFQKHADLFNDMPGEFNLPL 252
           CPL+ AVSLCNPFDLV  DE+F KGFNNVYDKAL++SL R F KH+ LF D+ GEFN+PL
Sbjct: 220 CPLTAAVSLCNPFDLVIADEDFHKGFNNVYDKALSKSLRRIFSKHSLLFEDIGGEFNIPL 279

Query: 253 AANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSSKVIKDVRIPLLCIQAANDPIAPDKAI 312
           AANA T+RD+DD LTRVSFGF SVDEYYSKSSSSK IK VRIPLLCIQAANDPIAP++ I
Sbjct: 280 AANAETVRDFDDGLTRVSFGFKSVDEYYSKSSSSKAIKHVRIPLLCIQAANDPIAPERGI 339

Query: 313 PREDIKENPNCLLIVTPKGGHLGWVAGSSAPLGAPWPDPFVIDFLVHLESQ 363
           PR+DIK NPNC+LIVTP+GGHLGWVAG  AP GAPW D  V++FL H+E++
Sbjct: 340 PRDDIKANPNCVLIVTPRGGHLGWVAGEEAPNGAPWTDQVVMEFLQHVENR 390

BLAST of CmaCh02G009420 vs. TAIR 10
Match: AT5G49950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 215.7 bits (548), Expect = 6.3e-56
Identity = 111/310 (35.81%), Postives = 176/310 (56.77%), Query Frame = 0

Query: 45  SNRHLETIFASFFRTSPKIKLRRECLRVADNGTIALDWVAGDHLRLPLD----------- 104
           S+ HL+T F + F  SP    +R   +  D GTIALDW+    +   +            
Sbjct: 87  SSPHLQTAFLTLFGKSPPFSYKRILYQATDGGTIALDWLMHSDVVEGISQVVNASNPGTD 146

Query: 105 -SPVLILLPGLTGGSQDSYVRHMLVRARDRSWRVVVFNNRGCGNSPVTSPQFYSASFLGD 164
            +P+ I++PGLT  S  +Y++H+  R     W VVV N+RG G   +TS   Y+A +  D
Sbjct: 147 RTPIAIIVPGLTSDSSAAYIKHIAFRLAKEGWNVVVQNHRGLGGISLTSDCVYTAGWTED 206

Query: 165 MRDVVAHVTTRYPEANVYAVGWSLGGNILVNYLGQDCMSCPLSGAVSLCNPFDLVDTDEN 224
           +R V+AH+ +++PEA ++AVG S+G N+LV YLG+D  + PL GA ++C+P+DL+  D  
Sbjct: 207 LRKVIAHIHSQFPEAPLFAVGTSIGANVLVKYLGEDGPNTPLIGATAVCSPWDLLICDRF 266

Query: 225 F-RKGFNNVYDKALARSLCRNFQKHADLFNDMPGEFNLPLAANAWTIRDYDDALTRVSFG 284
             RK    VYD+ L   L    Q H  + + +     +     + ++R++D+  TR+   
Sbjct: 267 INRKLVQKVYDRMLTIGLQGYAQLHHSIISRIADWEGI---KKSRSVREFDNYATRLVAK 326

Query: 285 FNSVDEYYSKSSSSKVIKDVRIPLLCIQAANDPIAPDKAIPREDIKENPNCLLIVTPKGG 342
           F + D YY +SSSS+ +++V +PLLCI A +DP+   +AIP ++ + N N +L  T  GG
Sbjct: 327 FETTDTYYRRSSSSQYVENVAVPLLCISALDDPVCTREAIPWDECRANKNIVLATTTHGG 386

BLAST of CmaCh02G009420 vs. TAIR 10
Match: AT1G34340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 204.1 bits (518), Expect = 1.9e-52
Identity = 119/364 (32.69%), Postives = 187/364 (51.37%), Query Frame = 0

Query: 31  KYLDKPYKKFPVIGSNRHLETIFASFFRTSPKIKLRRECLRVADNGTIALDWVAGD---- 90
           +Y+  P+   P      H++T F +F    P     R+    +D GTIALDW+       
Sbjct: 85  RYVATPWLASP------HIQTCFLNFHGLPPVFTYTRQLFLTSDGGTIALDWLTNSDVLD 144

Query: 91  ---HLRLPL----DSPVLILLPGLTGGSQDSYVRHMLVRARDRSWRVVVFNNRGCGNSPV 150
              H +  +     +P+ +++PGLT  S  +Y++H+        W VV+ N+RG G   V
Sbjct: 145 GSLHNKSEITKEDTTPIAVVIPGLTSDSSSAYLKHLAYDTAKTGWNVVISNHRGLGGVSV 204

Query: 151 TSPQFYSASFLGDMRDVVAHVTTRYPEANVYAVGWSLGGNILVNYLGQDCMSCPLSGAVS 210
           TS  FY+A +  D+R V+ ++  +YP A ++A+G S+G N+LV YLG++    PL GAV+
Sbjct: 205 TSDCFYNAGWTDDIRVVLDYLQHKYPRAPLFAIGTSIGANVLVKYLGEEGEKTPLRGAVA 264

Query: 211 LCNPFDLVDTDENF-RKGFNNVYDKALARSLCRNFQKHADLFNDMPGEFNLPLAANAWTI 270
           +C+P+DL+  D    R     +YDKAL   L    Q H   F  +    N      + +I
Sbjct: 265 ICSPWDLLIGDRFICRTLKQKLYDKALTIGLQGYAQLHEPQFLRLA---NWEGIKKSRSI 324

Query: 271 RDYDDALTRVSFGFNSVDEYYSKSSSSKVIKDVRIPLLCIQAANDPIAPDKAIPREDIKE 330
           RD+D+  T +   F +VD YY KSSS++ + +V +PLLCI A +DP+   +AIP ++ + 
Sbjct: 325 RDFDNHATCLVGKFETVDTYYRKSSSTQYVGNVAVPLLCISALDDPLCTKEAIPWDECRA 384

Query: 331 NPNCLLIVTPKGGHLG-----------WVAGSSAPLGAPWPDPFVIDFLVHLESQASKAS 372
           N N +L  T  GGHL            WV  ++  LG     P+     +H++    K S
Sbjct: 385 NKNIVLATTNHGGHLAFFEGLTGSSLWWVRATNEFLGVLSCSPY-----MHIQKIVDKRS 434

BLAST of CmaCh02G009420 vs. TAIR 10
Match: AT2G03140.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 154.1 bits (388), Expect = 2.3e-37
Identity = 87/273 (31.87%), Postives = 144/273 (52.75%), Query Frame = 0

Query: 63  IKLRRECLRVADNGTIALDWVAGDHLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRAR 122
           ++ +R C+ + D G ++LDW A   +R    LD+ V + +PG   GS +  VR  +  A 
Sbjct: 168 LEYQRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEAL 227

Query: 123 DRSWRVVVFNNRGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPEANVYAVGWSLGGNI 182
            R    VV N RGC  SP+T+P+ ++A    D+   +  ++   P   + AVG   G N+
Sbjct: 228 RRGVFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANM 287

Query: 183 LVNYLGQDCMSCPLSGAVSLCNPFDLVDTDENFRKGFNNVYDKALARSLCRNFQKHADLF 242
           L  YL +     PL+ AV + NPFDL +        ++   D+ L R L      + +LF
Sbjct: 288 LTKYLAEAGERTPLTAAVCIDNPFDLEEITRT--SPYSTSLDQQLTRGLVEILLANKELF 347

Query: 243 NDMPGEFNLPLAANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSSKVIKDVRIPLLCIQA 302
                 F++  A  + ++R++D AL+ V++G  S++++YS  ++ +VI +V++PLL IQ 
Sbjct: 348 QGRAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ- 407

Query: 303 ANDPIAPDKAIPREDIKENPNCLLIVTPKGGHL 334
            ND + P   IPR  I ENP   L++     +L
Sbjct: 408 -NDDVVPPYTIPRSSIAENPFTSLLLCSSSPNL 435

BLAST of CmaCh02G009420 vs. TAIR 10
Match: AT2G03140.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 154.1 bits (388), Expect = 2.3e-37
Identity = 87/273 (31.87%), Postives = 144/273 (52.75%), Query Frame = 0

Query: 63  IKLRRECLRVADNGTIALDWVAGDHLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRAR 122
           ++ +R C+ + D G ++LDW A   +R    LD+ V + +PG   GS +  VR  +  A 
Sbjct: 168 LEYQRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEAL 227

Query: 123 DRSWRVVVFNNRGCGNSPVTSPQFYSASFLGDMRDVVAHVTTRYPEANVYAVGWSLGGNI 182
            R    VV N RGC  SP+T+P+ ++A    D+   +  ++   P   + AVG   G N+
Sbjct: 228 RRGVFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANM 287

Query: 183 LVNYLGQDCMSCPLSGAVSLCNPFDLVDTDENFRKGFNNVYDKALARSLCRNFQKHADLF 242
           L  YL +     PL+ AV + NPFDL +        ++   D+ L R L      + +LF
Sbjct: 288 LTKYLAEAGERTPLTAAVCIDNPFDLEEITRT--SPYSTSLDQQLTRGLVEILLANKELF 347

Query: 243 NDMPGEFNLPLAANAWTIRDYDDALTRVSFGFNSVDEYYSKSSSSKVIKDVRIPLLCIQA 302
                 F++  A  + ++R++D AL+ V++G  S++++YS  ++ +VI +V++PLL IQ 
Sbjct: 348 QGRAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ- 407

Query: 303 ANDPIAPDKAIPREDIKENPNCLLIVTPKGGHL 334
            ND + P   IPR  I ENP   L++     +L
Sbjct: 408 -NDDVVPPYTIPRSSIAENPFTSLLLCSSSPNL 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q408632.5e-12662.78Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1[more]
Q0VC001.3e-5034.47Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1[more]
Q8WU672.3e-5034.47Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2[more]
Q91ZH76.6e-5034.13Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1[more]
Q54H386.8e-4735.65Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=ab... [more]
Match NameE-valueIdentityDescription
AT3G50790.11.1e-15672.08esterase/lipase/thioesterase family protein [more]
AT5G49950.16.3e-5635.81alpha/beta-Hydrolases superfamily protein [more]
AT1G34340.11.9e-5232.69alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.12.3e-3731.87alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.22.3e-3731.87alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012020AB hydrolase 4 familyPIRSFPIRSF005211Ab_hydro_YheTcoord: 20..371
e-value: 1.1E-117
score: 391.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 85..338
e-value: 5.4E-25
score: 90.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 58..342
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 95..333
e-value: 3.3E-18
score: 66.3
NoneNo IPR availablePANTHERPTHR10794ABHYDROLASE DOMAIN-CONTAINING PROTEINcoord: 13..367
NoneNo IPR availablePANTHERPTHR10794:SF81EMBRYOGENESIS-ASSOCIATED PROTEIN EMB8-LIKEcoord: 13..367
IPR000952AB hydrolase 4, conserved sitePROSITEPS01133UPF0017coord: 262..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G009420.1CmaCh02G009420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity