CmaCh02G008720 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh02G008720 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 9.5e-17
Identity = 75/282 (26.60%), Postives = 126/282 (44.68%), Query Frame = 0

Query: 163 LPKKRKVAALLRQITHLQARFDSCYPRLVARHDIKFAFKLAREAIVPQITQTEDPVPMKL 222
           +P +  V+ LLR   H++   +    R +   +     KL  +A VP + +    +  K 
Sbjct: 83  IPSEAAVSTLLR---HMKWNKEKLIERYMENPE-----KLCIDAGVPNVMKLNATIVEKS 142

Query: 223 -NETCVICYEDCDVNRMFAVDGCLHRYCFSCMKQHMEVKLLQG---LVPKCPHKGCMIDL 282
            N +C+IC ED    + FA+  C HRYC  C K ++E+K+ +G   +   CP   C + +
Sbjct: 143 GNVSCLICLEDYPPTQTFALI-CNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIV 202

Query: 283 NVDSCTKFLTPKDMATMRQHLKEASIPVSEKV-YCPYPKC--SALTTKGEALEYTRDVIG 342
           + D+  + ++P+        + ++ +  + +V +CP P C  S    + E  E       
Sbjct: 203 HQDAFKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGCIYSIRCDRKERKEAVN---- 262

Query: 343 SANQSGLRKCMKCHGLFCINC---KVPWHNLVSCSD---YKGSSNLELEDVKLKSLASTC 402
                      KC   +C NC   ++  H    CS    +   ++ E E+V    LA+T 
Sbjct: 263 ----------CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTW-MLANT- 322

Query: 403 LWRQCVKCNHMIELAEGCYHMTCR-----CGHQFCYKCGVEW 427
             ++C +C   IE   GC HMTCR     CG +FC+ C   W
Sbjct: 323 --KKCPECRSPIEKNGGCMHMTCRKNAGGCGFEFCWLCRGPW 337

BLAST of CmaCh02G008720 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 87.0 bits (214), Expect = 6.1e-16
Identity = 63/223 (28.25%), Postives = 94/223 (42.15%), Query Frame = 0

Query: 216 DPVPMKLNETCVICYEDCDVNRMFAVDGCLHRYCFSCMKQHMEVKLLQGLVP-KCPHKGC 275
           DP    +   C +C ED  +     +  C    C  C+K ++  ++  G V  KCP   C
Sbjct: 253 DPYVPLMVLMCRVCLEDKPIK---PLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITEC 312

Query: 276 MIDLNVDSCTKFLTPKDMATMRQHLKEASIPVSEKVYCPYPKCSALTT---KGEALEYTR 335
              L   +    LT +D    +  L+   I  S K   P P+C   TT   KG     +R
Sbjct: 313 FEFLEETTVVYNLTHEDSIKYKYFLELGRIDSSTK---PCPQCKHFTTFKKKGHIPTPSR 372

Query: 336 DVIGSANQSGLRKCMKCHGLFCINCKVPWHNLVSCSDYKGSSNL------ELEDVKLKSL 395
                +      +C  C  ++C  C  PWH  V+C +YK    L      E+E  +  + 
Sbjct: 373 -----SESKYKIQCPTCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEHGQRNA- 432

Query: 396 ASTCLWRQCVKCNHMIELAEGCYHMTC-RCGHQFCYKCGVEWK 428
                 ++C KC   I+  EGC HMTC +C   FCY+CG  ++
Sbjct: 433 ------QKCPKCKIHIQRTEGCDHMTCSQCNTNFCYRCGERYR 457

BLAST of CmaCh02G008720 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 8.0e-16
Identity = 63/223 (28.25%), Postives = 97/223 (43.50%), Query Frame = 0

Query: 216 DPVPMKLNETCVICYEDCDVNRMFAVDGCLHRYCFSCMKQHMEVKLLQGLVP-KCPHKGC 275
           DP    +   C +C ED  +     +  C    C  C+K ++  ++  G V  KCP   C
Sbjct: 226 DPYAPLMVLMCRVCLEDKPIK---PLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTEC 285

Query: 276 MIDLNVDSCTKFLTPKDMATMRQHLKEASIPVSEKVYCPYPKCSALTTKGEALEYTRDVI 335
              L   +    LT +D    +  L+   I  S K   P P+C   TT  +     +  I
Sbjct: 286 FEFLEETTVVYNLTHEDSIKYKYFLELGRIDSSTK---PCPQCKHFTTFKK-----KGHI 345

Query: 336 GSANQSGLR---KCMKCHGLFCINCKVPWHNLVSCSDYKGSSNL------ELEDVKLKSL 395
            + ++S  R   +C  C  ++C  C  PWH  V+C +YK    L      E+E  +  + 
Sbjct: 346 PTPSRSESRYKIQCPTCQLIWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEHGQRNA- 405

Query: 396 ASTCLWRQCVKCNHMIELAEGCYHMTC-RCGHQFCYKCGVEWK 428
                 ++C KC   I+  EGC HMTC +C   FCY+CG  ++
Sbjct: 406 ------QKCPKCKIHIQRTEGCDHMTCSQCNTNFCYRCGERYR 430

BLAST of CmaCh02G008720 vs. ExPASy Swiss-Prot
Match: Q4KLT0 (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.5e-14
Identity = 57/212 (26.89%), Postives = 98/212 (46.23%), Query Frame = 0

Query: 225 TCVICYEDCDVNRMFAVDGCLHRYCFSCMKQHM--EVKLLQGLVPKCPHKGCMIDLNVDS 284
           +C +C ED  +     +  C    C  C+K+++  +V+L Q  + +CP   C   L+  +
Sbjct: 2   SCRVCLEDRSIK---PLPCCKKPVCDECLKRYLSSQVQLGQAEI-QCPITECNKHLDEST 61

Query: 285 CTKFLTPKDMATMRQHLKEASIPVSEKVYCPYPKCSALTTKGEALEYTRDVIGSANQSGL 344
               L   D+   +  L+ + +  S K   P P+C   TT  +   +  +   S N+  +
Sbjct: 62  ILYSLPHDDIIKYKYFLELSRMDSSTK---PCPQCKHFTT-FKRKTHIPNPTKSENKLKI 121

Query: 345 RKCMKCHGLFCINCKVPWHNLVSCSDYKGSSNL------ELEDVKLKSLASTCLWRQCVK 404
            +C  C  ++C  C  PWH  V+C +YK    L      E+E  +  +       ++C +
Sbjct: 122 -QCPSCQFIWCFRCHAPWHEGVNCREYKKGDKLLRHWANEIEHGQRNA-------QKCPR 181

Query: 405 CNHMIELAEGCYHMTC-RCGHQFCYKCGVEWK 428
           C   I+  EGC HMTC +C   FCY+CG  ++
Sbjct: 182 CKVHIQRTEGCDHMTCSQCNTNFCYRCGERYR 197

BLAST of CmaCh02G008720 vs. ExPASy Swiss-Prot
Match: P0CE10 (ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01020 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 4.4e-14
Identity = 51/208 (24.52%), Postives = 83/208 (39.90%), Query Frame = 0

Query: 219  PMKLNETCVICYEDCDVNRMFAVDGCLHRYCFSCMKQHMEVKL--LQGLVPKCPHKGCMI 278
            P ++   C IC  + D    ++++GC H +C +C+ +  E  +         C H  C  
Sbjct: 1554 PDEIELECPICLSEVDDG--YSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGA 1613

Query: 279  DLNVDSCTKFLTPKDMATMRQHLKEASIPVSEKV--YCPYPKCSALTTKGEALEYTRDVI 338
             + V      L+ + +  +      A +  S+    +C  P C ++            V 
Sbjct: 1614 PIVVADMRALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSIYR----------VA 1673

Query: 339  GSANQSGLRKCMKCHGLFCINCKVPWHNLVSCSDYKGSSNLELEDVKLKSLASTCLWRQC 398
            G         C  CH   C  C + +H L++C  YK     E  D+ LK  A     ++C
Sbjct: 1674 GPQESGEPFICGACHSETCTRCHLEYHPLITCERYKKFK--ENPDLSLKDWAKGKDVKEC 1733

Query: 399  VKCNHMIELAEGCYHMTCRCGHQFCYKC 423
              C   IE  +GC H+ CRCG   C+ C
Sbjct: 1734 PICKSTIEKTDGCNHLQCRCGKHICWTC 1747

BLAST of CmaCh02G008720 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 363.2 bits (931), Expect = 3.2e-100
Identity = 211/560 (37.68%), Postives = 297/560 (53.04%), Query Frame = 0

Query: 6   LDSDHEFSYKLQIEEALDASLASSASS--FTPLPEFQELEGNDITTIGTLYSGELGECDQ 65
           +D D E++++LQ+EEAL ASL+S + +    P P         + T G L         +
Sbjct: 1   MDFDSEYAFRLQMEEALAASLSSQSRTPQRPPSPPI-------VATCGVLIVENNPNDSK 60

Query: 66  IFQEWLQSAFDTRR------------THDS---------------GEILNIPDG-----D 125
             +      FD +R            TH+S               G   +   G      
Sbjct: 61  TAKTSSGYGFDFKRAVGGGNSKAKGKTHESVTGVRTDERNPNIGLGNSRSTSTGHGNTKP 120

Query: 126 LSEREYSIGKSFGEGSSKSEENQG------VSSLYFKGLVSEE-----------GIGI-- 185
           L +      +  GEGSS+     G      +  LYFKGLVS+E           G G+  
Sbjct: 121 LPQETVRPAQLVGEGSSRVNTAAGKINGNLLYRLYFKGLVSDENGKGKMTDVVSGFGVAI 180

Query: 186 --------------------------------GLNAAIDLELKQLCFYCDYYSLYQFVTG 245
                                           GL  A+ L +K + F+CD Y ++Q+VTG
Sbjct: 181 CDQRDNLLFEMKGPLIDNGMSRQGAELKALIRGLTEALKLGIKHIVFFCDSYPIFQYVTG 240

Query: 246 RWLPKKRKVAALLRQITHLQARFDSCYPRLVARHDIKFAFKLAREAIVPQITQTEDPVPM 305
           +W+ K++K++ LL  +  +   F S    LVAR+D+KFA+KLARE+I+  +T  EDP   
Sbjct: 241 KWMAKQKKISLLLDDLQSIMQHFSSYQHVLVARNDVKFAYKLARESILSMVTPHEDPRQA 300

Query: 306 K--LNETCVICYEDCDVNRMFAVDGCLHRYCFSCMKQHMEVKLLQGLVPKCPHKGCMIDL 365
           K  L E C IC+ D     MF+VD C HR+CF C+KQH+EVKLL G+ PKCPH GC  +L
Sbjct: 301 KAVLKEECAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLHGMAPKCPHDGCKSEL 360

Query: 366 NVDSCTKFLTPKDMATMRQHLKEASIPVSEKVYCPYPKCSALTTKGEALEYTRDVIGSAN 425
            +D+C K LTPK     +Q L+E +IPV+E+VYCPYP+CSAL +K +  E  + ++    
Sbjct: 361 VIDACGKLLTPKLSKLWQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYP 420

Query: 426 QSGLRKCMKCHGLFCINCKVPWHNLVSCSDYKG-SSNLELEDVKLKSLASTCLWRQCVKC 475
           +SG+R+C++C GLFC++CKVPWH  +SC++YK        +DVKLKSLA+  +WRQC KC
Sbjct: 421 KSGVRRCVECRGLFCVDCKVPWHGNLSCTEYKKLHPEPPADDVKLKSLANNKMWRQCGKC 480

BLAST of CmaCh02G008720 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 3.0e-74
Identity = 139/309 (44.98%), Postives = 194/309 (62.78%), Query Frame = 0

Query: 125 VSEEGIGI-----GLNAAIDLELKQLCFYCDYYSLYQFVTGRWLPKKRKVAALLRQITHL 184
           +S  G+ I     GL+ + DL ++ +  YCD   +YQ + GR   KK K+  L+ ++  +
Sbjct: 203 ISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKK-KIDHLVEEVQGI 262

Query: 185 QARFDSCYPRLVARHDIKFAFKLAREAIVPQITQTEDPVPMKLNETCVICYEDCDVNRMF 244
             +       LVAR+D+KFAF+LAREA    I +    V  +  ETC I +E+ DV  MF
Sbjct: 263 LEKMACIDAVLVARNDVKFAFRLAREA----IGRNSVDVNAEQGETCGIFFEETDVEHMF 322

Query: 245 AVDGCLHRYCFSCMKQHMEVKLLQGLVPKCPHKGCMIDLNVDSCTKFLTPKDMATMRQHL 304
             + CLHR+CF C+KQH++VKL  G  P C   GC   L ++ C+K LT K +   +Q +
Sbjct: 323 VTEKCLHRHCFPCVKQHVKVKLRSGTEPTCLEYGCKFKLTLERCSKVLTLKLIEMWKQKM 382

Query: 305 KEASIPVSEKVYCPYPKCSALTTKGEALEYTRDVIGSANQSGLRKCMKCHGLFCINCKVP 364
           KE SIP +E++YCPYP CS L +K E       +   A+ S +R C+KC GLFCI+CKVP
Sbjct: 383 KEDSIPAAERIYCPYPNCSMLMSKTE-------LSSEADLSNVRTCVKCCGLFCIDCKVP 442

Query: 365 WHNLVSCSDYKG-SSNLELEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHQF 424
            H  +S  DYK    +  ++D+KLKSLA+  +WRQCVKC HMIEL+ GC HMTCRCG++F
Sbjct: 443 SHTDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYEF 499

Query: 425 CYKCGVEWK 428
           CY+CG+EW+
Sbjct: 503 CYECGIEWQ 499

BLAST of CmaCh02G008720 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 4.3e-73
Identity = 153/345 (44.35%), Postives = 204/345 (59.13%), Query Frame = 0

Query: 133 GLNAAIDLELKQLCFYCDYYSLYQFVTGRWLPKKRKVAALLRQITHLQARFDSCYPRLVA 192
           GL  + DL ++Q+  YCD   LYQ++ GR   KK KV  L+ +   L          L+A
Sbjct: 6   GLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKK-KVVHLVDEFQLLLEEMTYTDADLIA 65

Query: 193 RHDIKFAFKLAREAIVPQITQTEDPVPMKLNETCVICYEDCDVNRM-FAVDGCLHRYCFS 252
            +D+ FAFKLAREAIV     + D V     E C IC+E+ +  RM F  + C+HR+CF 
Sbjct: 66  LNDVNFAFKLAREAIV-----SRDDVKA---EICSICFEETEGERMFFTTEKCVHRHCFP 125

Query: 253 CMKQHMEVKLLQGLVPKCPHKGCMIDLNVDSCTKFLTPKDMATMRQHLKEASIPVSEKVY 312
           C+KQ++EVKLL G VP C   GC   L ++SC+K LT + +   +Q +KE SIP +E++Y
Sbjct: 126 CVKQYVEVKLLSGTVPTCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPAAERIY 185

Query: 313 CPYPKCSALTTKGEALEYTRDVIGSANQSGLRKCMKCHGLFCINCKVPWHNLVSCSDYKG 372
           CPYP CS L +K E       +   ++ S  R C+KC GLFCI+CKVP H+ +SC++YK 
Sbjct: 186 CPYPNCSMLMSKTE-------LSSESDLSNDRSCVKCCGLFCIDCKVPSHSDLSCAEYKK 245

Query: 373 SSNLEL-EDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHQFCYKCGVEWKVKK 432
             +  L +++KLKSLA    WRQC  C HMIEL+  C HMTCRCG+QFCY+C VEWK  +
Sbjct: 246 LHHDPLVDELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQCEVEWKNDQ 305

Query: 433 ATCS--CPLWGEDCIWNNDNNR-----ELDLDGIYLDEEEEEEEE 469
            TCS  C L G     + D N      E+D    Y DEE     E
Sbjct: 306 KTCSSGCLLTGHGYYDDYDYNEPEYDFEVDTCNYYSDEEAMTRRE 334

BLAST of CmaCh02G008720 vs. TAIR 10
Match: AT2G25370.2 (RING/U-box superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.9e-69
Identity = 144/351 (41.03%), Postives = 201/351 (57.26%), Query Frame = 0

Query: 99  IGKSFGEGSSKSEE--NQGVSSLYFKGLVSEEGIGIGLNAAIDL---------------- 158
           I  S GEGSS + +  +  V  L FKGLV+ E +   +  A+                  
Sbjct: 104 IPNSKGEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHE 163

Query: 159 ---ELKQLCFYCDYYSLYQFVTGRWLPKKRKVAALLRQITHLQARFDSCYPRLVARHDIK 218
               L+ +        +   + GR   KK K+  L+ ++  +  +       LVAR+D+K
Sbjct: 164 IKESLRDIEISRRGVEIMALIIGRGKSKK-KIDHLVEEVQGILEKMACIDAVLVARNDVK 223

Query: 219 FAFKLAREAIVPQITQTEDPVPMKLNETCVICYEDCDVNRMFAVDGCLHRYCFSCMKQHM 278
           FAF+LAREA    I +    V  +  ETC I +E+ DV  MF  + CLHR+CF C+KQH+
Sbjct: 224 FAFRLAREA----IGRNSVDVNAEQGETCGIFFEETDVEHMFVTEKCLHRHCFPCVKQHV 283

Query: 279 EVKLLQGLVPKCPHKGCMIDLNVDSCTKFLTPKDMATMRQHLKEASIPVSEKVYCPYPKC 338
           +VKL  G  P C   GC   L ++ C+K LT K +   +Q +KE SIP +E++YCPYP C
Sbjct: 284 KVKLRSGTEPTCLEYGCKFKLTLERCSKVLTLKLIEMWKQKMKEDSIPAAERIYCPYPNC 343

Query: 339 SALTTKGEALEYTRDVIGSANQSGLRKCMKCHGLFCINCKVPWHNLVSCSDYKG-SSNLE 398
           S L +K E       +   A+ S +R C+KC GLFCI+CKVP H  +S  DYK    +  
Sbjct: 344 SMLMSKTE-------LSSEADLSNVRTCVKCCGLFCIDCKVPSHTDLSYDDYKKLHPDPL 403

Query: 399 LEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHQFCYKCGVEWK 428
           ++D+KLKSLA+  +WRQCVKC HMIEL+ GC HMTCRCG++FCY+CG+EW+
Sbjct: 404 VDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYEFCYECGIEWQ 442

BLAST of CmaCh02G008720 vs. TAIR 10
Match: AT3G45580.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 260.0 bits (663), Expect = 3.8e-69
Identity = 140/371 (37.74%), Postives = 201/371 (54.18%), Query Frame = 0

Query: 106 GSSKSEENQGVSSLYFKGLVSEE------GIGI--------------------------- 165
           G   SE       LYFKGLVSEE      G G+                           
Sbjct: 17  GRVSSEIKPDSYRLYFKGLVSEETVELLAGFGVAICDKDDNLLFQMKEQVHDSRVTVLEV 76

Query: 166 -------GLNAAIDLELKQLCFYCDYYSLYQFVTGRWLPKKRKVAALLRQITHLQARFDS 225
                  GL  A+ L +  +  Y D+Y +++ V  +    +   A L+  + H++ R  S
Sbjct: 77  EIMALKRGLTEAVGLGIDNISIYSDHYRIFELVMEKSASAEENFALLMDNVQHIRQRLTS 136

Query: 226 CYPRLVARHDIKFAFKLAREAIVPQITQTEDPVPMKLNETCVICYED-CDVNRMFAVDGC 285
            +P LV R+ IKF ++LA E IV +I+     +P   ++TC IC +D  +   MF+V  C
Sbjct: 137 SFPVLVTRNQIKFVYELAMETIVSEIS---IHIP-DHDKTCSICSDDNFEPELMFSVALC 196

Query: 286 LHRYCFSCMKQHMEVKLLQGLVPKCPHKGCMIDLNVDSCTKFLTPKDMATMRQHLKEASI 345
            H +C  C+K+H+EV+LL G VP+C H  C   L + +C   LT K  A     ++E SI
Sbjct: 197 GHEFCVECVKRHIEVRLLAGGVPRCLHYQCESKLTLANCANLLTSKLKAMWELRIEEESI 256

Query: 346 PVSEKVYCPYPKCSALTTKGEALEYTRDVIGSANQSGLRKCMKCHGLFCINCKVPWHNLV 405
           PV E+VYCP P+CS+L +  +    TR+ +       +R C+KC   FCINCK+PWH+ +
Sbjct: 257 PVEERVYCPNPRCSSLMSVTKLSNSTREDV------TMRSCVKCGEPFCINCKLPWHSNL 316

Query: 406 SCSDYKG-SSNLELEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHQFCYKCG 435
           SC+DYK    N   +D+KLK+LA+  +WRQC  C ++IEL+EGC H+TCRCGHQFCYKCG
Sbjct: 317 SCNDYKSLGPNPTADDIKLKALANQKMWRQCENCKNVIELSEGCMHITCRCGHQFCYKCG 376