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Sequences
The following sequences are available for this feature:
Gene sequence (with intron) Legend: exonCDSpolypeptide Hold the cursor over a type above to highlight its positions in the sequence below. ATGGGCAAGTCTGAAGGAGATGAGAAGATCTTAAGCTACAATGATGTTGTACTCAGAAAATCAGACTTAGACATCCTTAATGGTCCATGTTATCTGAATGATCGAATCATCGAGTTCTATTTTAGCTATCTATGCACATGCCATCCTTCCGAGGACATCCTTCTATCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCGGACACCGACGGTCTCAATGATTTCCTACAACCACTCAATCTACGCGTCAGGAATCTGGTAATCTTCCCAGTCAATGACAACAATGATGTTAGTAAAGCTGAAGGTGGAAACCACTGGAGTGTTCTTGTATTTTACAGAGAAGCAAACGTTTTTGTTCATCATGATAGCTGCAAAGGGGTGAATAATTCCTCCGCCAAGCGACTCTACAGAGCTGTGGCTAGATTCATGGGTGTTACGAATTCGACATCCGAACCCAGTTATGTGGAATGCAGTGAGTCTCCTCAGCAAGTAAATGGACATGATTGTGGTTTGTATGTTACAGCCATATCAAGAAGTATATGCAGGTGGTATGAGCAGGAGAGGAAAGAGGCTGGTGGTGGAGATGGTTTTTTGTGGTTTTCTGCAGTGGTGAAGGAGGTTACCCCATCTTCGGTTGCTGATATGCGTAGTGAAATCTTAGGGCTAATACGAAGTTTCATGGCTCTTAAGGAATACCAACCGGCTTCTAAGTAA mRNA sequence ATGGGCAAGTCTGAAGGAGATGAGAAGATCTTAAGCTACAATGATGTTGTACTCAGAAAATCAGACTTAGACATCCTTAATGGTCCATGTTATCTGAATGATCGAATCATCGAGTTCTATTTTAGCTATCTATGCACATGCCATCCTTCCGAGGACATCCTTCTATCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCGGACACCGACGGTCTCAATGATTTCCTACAACCACTCAATCTACGCGTCAGGAATCTGGTAATCTTCCCAGTCAATGACAACAATGATGTTAGTAAAGCTGAAGGTGGAAACCACTGGAGTGTTCTTGTATTTTACAGAGAAGCAAACGTTTTTGTTCATCATGATAGCTGCAAAGGGGTGAATAATTCCTCCGCCAAGCGACTCTACAGAGCTGTGGCTAGATTCATGGGTGTTACGAATTCGACATCCGAACCCAGTTATGTGGAATGCAGTGAGTCTCCTCAGCAAGTAAATGGACATGATTGTGGTTTGTATGTTACAGCCATATCAAGAAGTATATGCAGGTGGTATGAGCAGGAGAGGAAAGAGGCTGGTGGTGGAGATGGTTTTTTGTGGTTTTCTGCAGTGGTGAAGGAGGTTACCCCATCTTCGGTTGCTGATATGCGTAGTGAAATCTTAGGGCTAATACGAAGTTTCATGGCTCTTAAGGAATACCAACCGGCTTCTAAGTAA Coding sequence (CDS) ATGGGCAAGTCTGAAGGAGATGAGAAGATCTTAAGCTACAATGATGTTGTACTCAGAAAATCAGACTTAGACATCCTTAATGGTCCATGTTATCTGAATGATCGAATCATCGAGTTCTATTTTAGCTATCTATGCACATGCCATCCTTCCGAGGACATCCTTCTATCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCGGACACCGACGGTCTCAATGATTTCCTACAACCACTCAATCTACGCGTCAGGAATCTGGTAATCTTCCCAGTCAATGACAACAATGATGTTAGTAAAGCTGAAGGTGGAAACCACTGGAGTGTTCTTGTATTTTACAGAGAAGCAAACGTTTTTGTTCATCATGATAGCTGCAAAGGGGTGAATAATTCCTCCGCCAAGCGACTCTACAGAGCTGTGGCTAGATTCATGGGTGTTACGAATTCGACATCCGAACCCAGTTATGTGGAATGCAGTGAGTCTCCTCAGCAAGTAAATGGACATGATTGTGGTTTGTATGTTACAGCCATATCAAGAAGTATATGCAGGTGGTATGAGCAGGAGAGGAAAGAGGCTGGTGGTGGAGATGGTTTTTTGTGGTTTTCTGCAGTGGTGAAGGAGGTTACCCCATCTTCGGTTGCTGATATGCGTAGTGAAATCTTAGGGCTAATACGAAGTTTCATGGCTCTTAAGGAATACCAACCGGCTTCTAAGTAA Protein sequence MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSIAFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFVHHDSCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRSICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK
Homology
BLAST of CmaCh02G008650 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1) HSP 1 Score: 266.5 bits (680), Expect = 2.8e-70 Identity = 131/231 (56.71%), Postives = 167/231 (72.29%), Query Frame = 0
Query: 1 MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60
MG + D+KILSY DVVLR+SDLDILNGP +LNDR+IEFY S+L T H S I L PPSI
Sbjct: 1 MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60
Query: 61 AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120
AFWI NCPDT+ L DF++PLNLR ++L+I PVNDN++V AEGG HWS+LV+Y+EAN FV
Sbjct: 61 AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120
Query: 121 HHDSCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRS 180
HHDS GVN SAK+L++AV+ F+ S + SY EC+++PQQ NG+DCG+++ A +R
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV----SNGDASYKECTDTPQQKNGYDCGVFLLATARV 180
Query: 181 ICRWYEQERKEAGG--GDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMA 230
IC W+ +GG LWF A VKE P V +R EIL LI+ M+
Sbjct: 181 ICEWF-----SSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221
BLAST of CmaCh02G008650 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1) HSP 1 Score: 123.6 bits (309), Expect = 2.9e-27 Identity = 75/240 (31.25%), Postives = 127/240 (52.92%), Query Frame = 0
Query: 7 DEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYL-------CTCHPSEDILLSPPS 66
D I+SYND + +SD++IL +LND II FY +L P++ +LLS PS
Sbjct: 3 DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLS-PS 62
Query: 67 IAFWIMNCPDTDGLN-DFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANV 126
+ F + F QPL+L+ + ++ FP+ +N D + GG HWS+L+F + N
Sbjct: 63 VVFCCSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNK 122
Query: 127 FVHHDSCKGVNNSSAKRLYRAVARFMGVTNS----TSEPSYVECSESPQQVNGHDCGLYV 186
F+++DS N+S A + +++F + +S T+ ++ ++PQQ NG+DCGLYV
Sbjct: 123 FIYYDSINSFNSSDAIFI---ISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYV 182
Query: 187 TAISRSICRWYEQERKEAGGGDGFLWFSAVV----------KEVTPSSVADMRSEILGLI 225
+I + + +E + G + + + ++ KE+TP+ + D R EIL I
Sbjct: 183 LSIIEELLKLIIKENENNKGEENKISYKDLLLSEFTNELLFKEITPNYIKDKRVEILNTI 238
BLAST of CmaCh02G008650 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1) HSP 1 Score: 116.3 bits (290), Expect = 4.7e-25 Identity = 67/221 (30.32%), Postives = 120/221 (54.30%), Query Frame = 0
Query: 7 DEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCH---PSEDILLSPPSIAFW 66
D +LSY D +LR+SD+ +L+ P +LND II F F Y S+ + P + +
Sbjct: 2 DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQF 61
Query: 67 IMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFVHHD 126
I + + FL+PL+L + +V +NDN+ ++A GG HWS+LV+ ++ N F H+D
Sbjct: 62 IKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121
Query: 127 SCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRSICR 186
S N+ AK++ + F+G + ++VE ++P Q N +DCG+YV + ++C+
Sbjct: 122 SHSRSNSVHAKQVAEKLEAFLG--RKGDKLAFVE-EKAPAQQNSYDCGMYVICNTEALCQ 181
Query: 187 WYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLI 225
+ +++ E ++++ +TP+ + R E LI
Sbjct: 182 NFFRQQTE-----------SLLQLLTPAYITKKRGEWKDLI 206
BLAST of CmaCh02G008650 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2) HSP 1 Score: 111.3 bits (277), Expect = 1.5e-23 Identity = 60/180 (33.33%), Postives = 101/180 (56.11%), Query Frame = 0
Query: 7 DEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCH---PSEDILLSPPSIAFW 66
D +LSY D +LR+SD+ +L+ P +LND II F F Y S+ + P + +
Sbjct: 2 DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQF 61
Query: 67 IMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFVHHD 126
I + FL+PL+L + +V +NDN+ ++A GG HWS+LV+ ++ N F H+D
Sbjct: 62 IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121
Query: 127 SCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRSICR 184
S N+ AK++ + F+G + + +VE ++P Q N +DCG+YV + ++C+
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLG--SKGDKLVFVE-EKAPAQENSYDCGMYVICNTEALCQ 176
BLAST of CmaCh02G008650 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1) HSP 1 Score: 110.9 bits (276), Expect = 2.0e-23 Identity = 60/180 (33.33%), Postives = 102/180 (56.67%), Query Frame = 0
Query: 7 DEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCH---PSEDILLSPPSIAFW 66
D +LSY D +LR+SD+ +L+ P +LND II F F Y + S+ + P + +
Sbjct: 2 DPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQF 61
Query: 67 IMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFVHHD 126
I + FL+PL+L + +V +NDN+ ++A GG HWS+LV+ ++ N F H+D
Sbjct: 62 IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121
Query: 127 SCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRSICR 184
S N+ AK++ + F+G + + +VE ++P Q N +DCG+YV + ++C+
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLG--SKGDKLVFVE-EKAPAQQNSYDCGMYVICNTEALCQ 176
BLAST of CmaCh02G008650 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein ) HSP 1 Score: 266.5 bits (680), Expect = 2.0e-71 Identity = 131/231 (56.71%), Postives = 167/231 (72.29%), Query Frame = 0
Query: 1 MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60
MG + D+KILSY DVVLR+SDLDILNGP +LNDR+IEFY S+L T H S I L PPSI
Sbjct: 1 MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60
Query: 61 AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120
AFWI NCPDT+ L DF++PLNLR ++L+I PVNDN++V AEGG HWS+LV+Y+EAN FV
Sbjct: 61 AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120
Query: 121 HHDSCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRS 180
HHDS GVN SAK+L++AV+ F+ S + SY EC+++PQQ NG+DCG+++ A +R
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV----SNGDASYKECTDTPQQKNGYDCGVFLLATARV 180
Query: 181 ICRWYEQERKEAGG--GDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMA 230
IC W+ +GG LWF A VKE P V +R EIL LI+ M+
Sbjct: 181 ICEWF-----SSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221
BLAST of CmaCh02G008650 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein ) HSP 1 Score: 46.6 bits (109), Expect = 3.2e-05 Identity = 51/210 (24.29%), Postives = 79/210 (37.62%), Query Frame = 0
Query: 16 VVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSIA-FWIMNC------- 75
VV+RK D+++L ++ND II+FY YL +SP F NC
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKN-------RISPKERGRFHFFNCFFFRKLA 364
Query: 76 ------PDTDGLNDFLQPLNLRVRNLVIF-------PVNDNNDVSKAEGGNHWSVLVFYR 135
P T G + Q + +N+ +F P+N + HWS+++
Sbjct: 365 NLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS---------FHWSLVIICH 424
Query: 136 EANV----------------------FVHHDSCKGVNNSSAKRLYRAV------ARFMGV 175
+ +H DS KG + ++ + AR
Sbjct: 425 PGELVPSHVNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENT 484
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9LSS7 | 2.8e-70 | 56.71 | NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1 | [more] |
| Q54XR2 | 2.9e-27 | 31.25 | Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen... | [more] |
| Q96LD8 | 4.7e-25 | 30.32 | Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1 | [more] |
| Q9D2Z4 | 1.5e-23 | 33.33 | Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2 | [more] |
| Q5FVJ8 | 2.0e-23 | 33.33 | Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G60190.1 | 2.0e-71 | 56.71 | Cysteine proteinases superfamily protein | [more] |
| AT4G33620.1 | 3.2e-05 | 24.29 | Cysteine proteinases superfamily protein | [more] |
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
| IPR Term | IPR Description | Source | Source Term | Source Description | Alignment |
|---|
| IPR003653 | Ulp1 protease family, C-terminal catalytic domain | PFAM | PF02902 | Peptidase_C48 | coord: 86..178
e-value: 1.3E-7
score: 31.7 |
| IPR003653 | Ulp1 protease family, C-terminal catalytic domain | PROSITE | PS50600 | ULP_PROTEASE | coord: 16..181
score: 12.401539 |
| None | No IPR available | GENE3D | 3.40.395.10 | Adenoviral Proteinase; Chain A | coord: 7..229
e-value: 3.4E-61
score: 208.7 |
| None | No IPR available | PANTHER | PTHR46468:SF4 | BNAA10G29630D PROTEIN | coord: 2..229 |
| IPR044613 | NEDD8-specific protease 1/2-like | PANTHER | PTHR46468 | SENTRIN-SPECIFIC PROTEASE 8 | coord: 2..229 |
| IPR038765 | Papain-like cysteine peptidase superfamily | SUPERFAMILY | 54001 | Cysteine proteinases | coord: 7..227 |
Relationships
The following mRNA feature(s) are a part of this gene:
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
| Category |
Term Accession |
Term Name |
| biological_process |
GO:0006508 |
proteolysis |
| molecular_function |
GO:0019784 |
NEDD8-specific protease activity |
| molecular_function |
GO:0008234 |
cysteine-type peptidase activity |
|
