CmaCh02G001850 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh02G001850
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein NRT1/ PTR FAMILY 6.4
LocationCma_Chr02: 855122 .. 856986 (-)
RNA-Seq ExpressionCmaCh02G001850
SyntenyCmaCh02G001850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCATATCAATGAACTTAGTGACATATCTACTTTGGGATCTGCATCTCTCTTCAGCTAAGTCTGCAACTATTGTGACTAATTTTCTTGGAGCCCTTAATCTTCTTGGCCTTCTTGGTGGCTTCTTAGCTGATGCCAAGCTGGGACGGTTCCTCACTGTTGCAACCTCTGCTTCCATAACTGCTGTGGTAAGTTCTGAGCCATTAAGAATGGCTTCAACAGAGAAATTTCTGCTTTATCCTCAGCTTTCCCGGTTTTTGCAAGGTGTGATTTTGTTAACACTGGCCACAACCATCCCTGGCATGAGACCTCCTCATTGTGATATCTCCTCCACAAGGCAACTTCATCAATGCATTGCGGCTAATGGTGGGCAACTTGCCATGCTCTATGCTGCACTTTATACCATAGCACTCGGCGGCGGGGGGATAAAATCAAATGTGTCAGGATTCAGGTCCGATCAATTTGATGTCAACGATCCAAAGGAAGAGAAGGCTATGTTATTCTTCTTCAATAGATTCTACTTTGCCATAAGCATTAGATCGTTGTTTGCTGTGATTGTGTTAGTTTATGTACAAGACTATGTGGGAAGAGGCTGGGGCTATGGAATCTCAGGAGGGACCATGGTGATTGCTGTAATTGTATTGGTTTGTGGGACAACATTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTAGTGCTTCTGGCTTGGAAGAAGAGAAGCCTCCCTCATCCAGTTCATCCCAGGTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCATGTAAGTGTTCTTCAGATCTATAGGCTAGCTATAAATGGAAAAAAGTAGATTTGTTCGTCCAATCCACAAGTAAATTGGTGGATTCAATTCAGTTAATTTATTTGGTTATCTTTATCATTTTGGCAAGGAAATAGTGTAATGAAAACGTGAATATTAGGTGTCTAGACAAGGCTGCAATTCTGGACGAGGTTGCAGCGATCAATCAAAACAGATATAATCCCTGGATAGTCTCGACCGTGACTGAAGTCGAGGAAGTGAAAATGGTTCTGAAGCTTTTACCCATATGGTCCACTGGCATCCTCTTTTGGGCGATCTACTCTCAGATGACTGACTACATTCACAGTAGAGCAAGCATCATTTATGGAGAGAAAAGTTGGCAAGTTCGTGATTCCTGCTGGTTCTATGTAAGCCTTTCTATTCATCGCCATCCTGCTAGTCACTTCCATGAACGAGAAACTGTTCATTCCCTTTTCTCGGAAACTGACTCTCAATGTCCAAGGTCTCACAAGCCTACAGAGGATTGGGATTGGCGTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGATTTGTTGAGAAGGAGAGGAGGGGGTTTGCTGTGGGACAAAATACTCTTATAAGTGTTTTCTGGTTAATACCTCAGTTCTTCTTGGTTGGTGCTGGAGAGGCATTTGCATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCAGAACGAATGAAATCCATGAGCACAGGACTTTTCCTTAGCACCCTTTCCATGGGATTTTTTGTAAGTAGTTTGTTGGTGACGATCGTAGAAAACGTAACCAACAAGCAATGGCTGAAAAGTAATCTCAACAAGGGAAACTTAAACTACTTCTATTGGCTACTTGCAGTGTTGGGACTGCTGAACTTTTTATTTTTTCTTCTACTTGCCCAGAAACATCAGTACAAAGACCAGCATTATTCCAGTTCTAATGACAACGATGAGGAGCTCAAGAGTTCAAGTAACATGATCGCACATGAAATGGAAGGACCAATTAGCATGAAGGCAACAGAAACCTAG

mRNA sequence

ATGGGCATATCAATGAACTTAGTGACATATCTACTTTGGGATCTGCATCTCTCTTCAGCTAAGTCTGCAACTATTGTGACTAATTTTCTTGGAGCCCTTAATCTTCTTGGCCTTCTTGGTGGCTTCTTAGCTGATGCCAAGCTGGGACGGTTCCTCACTGTTGCAACCTCTGCTTCCATAACTGCTGTGGTAAGTTCTGAGCCATTAAGAATGGCTTCAACAGAGAAATTTCTGCTTTATCCTCAGCTTTCCCGGTTTTTGCAAGGTGTGATTTTGTTAACACTGGCCACAACCATCCCTGGCATGAGACCTCCTCATTGTGATATCTCCTCCACAAGGCAACTTCATCAATGCATTGCGGCTAATGGTGGGCAACTTGCCATGCTCTATGCTGCACTTTATACCATAGCACTCGGCGGCGGGGGGATAAAATCAAATGTGTCAGGATTCAGGTCCGATCAATTTGATGTCAACGATCCAAAGGAAGAGAAGGCTATGTTATTCTTCTTCAATAGATTCTACTTTGCCATAAGCATTAGATCGTTGTTTGCTGTGATTGTGTTAGTTTATGTACAAGACTATGTGGGAAGAGGCTGGGGCTATGGAATCTCAGGAGGGACCATGGTGATTGCTGTAATTGTATTGGTTTGTGGGACAACATTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTAGTGCTTCTGGCTTGGAAGAAGAGAAGCCTCCCTCATCCAGTTCATCCCAGGTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCATGTGTCTAGACAAGGCTGCAATTCTGGACGAGGTTGCAGCGATCAATCAAAACAGATATAATCCCTGGATAGTCTCGACCGTGACTGAAGTCGAGGAAGTGAAAATGGTTCTGAAGCTTTTACCCATATGGTCCACTGGCATCCTCTTTTGGGCGATCTACTCTCAGATGACTGACTACATTCACAGTAGAGCAAGCATCATTTATGGAGAGAAAAGTTGGCAAGTTCTCACTTCCATGAACGAGAAACTGTTCATTCCCTTTTCTCGGAAACTGACTCTCAATGTCCAAGGTCTCACAAGCCTACAGAGGATTGGGATTGGCGTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGATTTGTTGAGAAGGAGAGGAGGGGGTTTGCTGTGGGACAAAATACTCTTATAAGTGTTTTCTGGTTAATACCTCAGTTCTTCTTGGTTGGTGCTGGAGAGGCATTTGCATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCAGAACGAATGAAATCCATGAGCACAGGACTTTTCCTTAGCACCCTTTCCATGGGATTTTTTGTAAGTAGTTTGTTGGTGACGATCGTAGAAAACGTAACCAACAAGCAATGGCTGAAAAGTAATCTCAACAAGGGAAACTTAAACTACTTCTATTGGCTACTTGCAGTGTTGGGACTGCTGAACTTTTTATTTTTTCTTCTACTTGCCCAGAAACATCAGTACAAAGACCAGCATTATTCCAGTTCTAATGACAACGATGAGGAGCTCAAGAGTTCAAGTAACATGATCGCACATGAAATGGAAGGACCAATTAGCATGAAGGCAACAGAAACCTAG

Coding sequence (CDS)

ATGGGCATATCAATGAACTTAGTGACATATCTACTTTGGGATCTGCATCTCTCTTCAGCTAAGTCTGCAACTATTGTGACTAATTTTCTTGGAGCCCTTAATCTTCTTGGCCTTCTTGGTGGCTTCTTAGCTGATGCCAAGCTGGGACGGTTCCTCACTGTTGCAACCTCTGCTTCCATAACTGCTGTGGTAAGTTCTGAGCCATTAAGAATGGCTTCAACAGAGAAATTTCTGCTTTATCCTCAGCTTTCCCGGTTTTTGCAAGGTGTGATTTTGTTAACACTGGCCACAACCATCCCTGGCATGAGACCTCCTCATTGTGATATCTCCTCCACAAGGCAACTTCATCAATGCATTGCGGCTAATGGTGGGCAACTTGCCATGCTCTATGCTGCACTTTATACCATAGCACTCGGCGGCGGGGGGATAAAATCAAATGTGTCAGGATTCAGGTCCGATCAATTTGATGTCAACGATCCAAAGGAAGAGAAGGCTATGTTATTCTTCTTCAATAGATTCTACTTTGCCATAAGCATTAGATCGTTGTTTGCTGTGATTGTGTTAGTTTATGTACAAGACTATGTGGGAAGAGGCTGGGGCTATGGAATCTCAGGAGGGACCATGGTGATTGCTGTAATTGTATTGGTTTGTGGGACAACATTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTAGTGCTTCTGGCTTGGAAGAAGAGAAGCCTCCCTCATCCAGTTCATCCCAGGTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCATGTGTCTAGACAAGGCTGCAATTCTGGACGAGGTTGCAGCGATCAATCAAAACAGATATAATCCCTGGATAGTCTCGACCGTGACTGAAGTCGAGGAAGTGAAAATGGTTCTGAAGCTTTTACCCATATGGTCCACTGGCATCCTCTTTTGGGCGATCTACTCTCAGATGACTGACTACATTCACAGTAGAGCAAGCATCATTTATGGAGAGAAAAGTTGGCAAGTTCTCACTTCCATGAACGAGAAACTGTTCATTCCCTTTTCTCGGAAACTGACTCTCAATGTCCAAGGTCTCACAAGCCTACAGAGGATTGGGATTGGCGTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGATTTGTTGAGAAGGAGAGGAGGGGGTTTGCTGTGGGACAAAATACTCTTATAAGTGTTTTCTGGTTAATACCTCAGTTCTTCTTGGTTGGTGCTGGAGAGGCATTTGCATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCAGAACGAATGAAATCCATGAGCACAGGACTTTTCCTTAGCACCCTTTCCATGGGATTTTTTGTAAGTAGTTTGTTGGTGACGATCGTAGAAAACGTAACCAACAAGCAATGGCTGAAAAGTAATCTCAACAAGGGAAACTTAAACTACTTCTATTGGCTACTTGCAGTGTTGGGACTGCTGAACTTTTTATTTTTTCTTCTACTTGCCCAGAAACATCAGTACAAAGACCAGCATTATTCCAGTTCTAATGACAACGATGAGGAGCTCAAGAGTTCAAGTAACATGATCGCACATGAAATGGAAGGACCAATTAGCATGAAGGCAACAGAAACCTAG

Protein sequence

MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASITAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRSLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSNDNDEELKSSSNMIAHEMEGPISMKATET
Homology
BLAST of CmaCh02G001850 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.8e-182
Identity = 339/534 (63.48%), Postives = 397/534 (74.34%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGR+  VA SAS+
Sbjct: 51  MGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASV 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TA+                         GV+LLT+ATTI  MRPP CD    R  HQCI 
Sbjct: 111 TAL-------------------------GVLLLTVATTISSMRPPICD-DFRRLHHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANG QLA+LY ALYTIALGGGGIKSNVSGF SDQFD +DPKEEK M+FFFNRFYF+IS+ 
Sbjct: 171 ANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVI LVYVQD VGRGWGYGIS  TMV+A IVL+CGT  YRFKKP+GSP T IWRV  
Sbjct: 231 SLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGF 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRY---NPWIVS 300
           LAWKKR   +P HP  LN Y N  VP+T    CLDKAAI    ++ +   +   +PWIVS
Sbjct: 291 LAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDKAAISKNESSPSSKDFEEKDPWIVS 350

Query: 301 TVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-----------KSWQ 360
           TVT+VEEVK+V+KL+PIW+T ILFW IYSQMT +   +A+ +  +            ++ 
Sbjct: 351 TVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAFL 410

Query: 361 VL-----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFA 420
           +L     TS+NE++F+P +R+LT   QG+TSLQRIG+G+VFS+  M  A  +E  RR  A
Sbjct: 411 ILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAVAAVIENARREAA 470

Query: 421 VGQNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFV 480
           V  +  IS FWL+PQ+FLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLST+SMGFFV
Sbjct: 471 VNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTISMGFFV 530

Query: 481 SSLLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYK 516
           SSLLV++V+ VT+K WL+SNLNK  LNYFYWLL VLG LNFL F++ A KHQYK
Sbjct: 531 SSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of CmaCh02G001850 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.5e-116
Identity = 243/551 (44.10%), Postives = 331/551 (60.07%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           +GI +NLVTYL   +HL +A +A  VTNFLG   +L LLGGF+AD  LGR+LT+A  A+I
Sbjct: 49  LGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAI 108

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A                          GV +LTL+T IPG+RPP C+ +++     C  
Sbjct: 109 QAT-------------------------GVSILTLSTIIPGLRPPRCNPTTS---SHCEQ 168

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A+G QL +LY ALY  ALG GG+K++VSGF SDQFD  +PKE   M +FFNRF+F I++ 
Sbjct: 169 ASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVG 228

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SL AV VLVYVQD VGR WGYGI    +V+A+ V + GT  YRFKK  GSP+T +  V++
Sbjct: 229 SLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIV 288

Query: 241 LAWKKRSLPHPVHPRFLNDYQN-----------AKVPYTYRFMCLDKAAILDEVAAINQN 300
            AW+ R L  P  P +L D  +            K+P+T +F  LDKAAI D+ A +  N
Sbjct: 289 AAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSN 348

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY----------------IH 360
            +N W +ST+T+VEEVK ++++LPIW+T ILFW +++Q+T                  I 
Sbjct: 349 VFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIP 408

Query: 361 SRASIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
             +  ++      + T++ +++ I   +KL     GL  LQRIG+G+ F    M  A  V
Sbjct: 409 PASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALV 468

Query: 421 EKERRGFAVGQNTLISV-----FWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMST 480
           E +R   A      +       + LIPQ+ +VG GEA  Y GQL+FF+RE P+ MK MST
Sbjct: 469 ELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMST 528

Query: 481 GLFLSTLSMGFFVSSLLVTIVENVTNK--QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 518
           GL LSTL++GFF SS+LVTIVE  T K   W+  +LNKG L  FYWL+AVL  LNFL FL
Sbjct: 529 GLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFL 571

BLAST of CmaCh02G001850 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.1e-112
Identity = 246/543 (45.30%), Postives = 338/543 (62.25%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGI++NLVTYL+  +HL S+ SA IVT+F+G   LL LLGGFLAD+ LGRF T+   ++I
Sbjct: 46  MGIAVNLVTYLMETMHLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTI 105

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A+                         G   L +AT +P +RPP C          CI 
Sbjct: 106 QAL-------------------------GTGALAVATKLPELRPPTCHHGEA-----CIP 165

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A   Q+ +LY +LY IALG GG+KS++SGF SDQFD  DPKE+  M FFFNRF+F IS+ 
Sbjct: 166 ATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISMG 225

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           +L AV VLVY+QD VGR W YGI   +M IA+++ +CGT  YR+KK +GSP+  I++V+ 
Sbjct: 226 TLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVIA 285

Query: 241 LAWKKR--SLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAI---------LDEVAAINQN 300
            A++KR   LP  +   + ++ +  ++ +T +F  LDKAAI         LD VA     
Sbjct: 286 AAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLDGVAI---- 345

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRAS------------ 360
             NPW +S+VT+VEEVKM+++LLPIW+T I+FW  Y+QM  +   +AS            
Sbjct: 346 -PNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSFKIP 405

Query: 361 ----IIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
                ++   +  +  ++ ++  +PF +K      G +SLQRI IG+V S  GM AA  V
Sbjct: 406 AGSLTVFFVAAILITLAVYDRAIMPFWKKWK-GKPGFSSLQRIAIGLVLSTAGMAAAALV 465

Query: 421 EKERRGFA--VGQNTL-ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 480
           E++R   A    Q TL ISVF L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK+MSTGL
Sbjct: 466 EQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMSTGL 525

Query: 481 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 510
           FL+TLS+GFFVSS LV+IV+ VT+      WL  N+N G L+YFYWLL +L  +NF+ ++
Sbjct: 526 FLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFVVYI 552

BLAST of CmaCh02G001850 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.2e-95
Identity = 207/549 (37.70%), Postives = 314/549 (57.19%), Query Frame = 0

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+  NLV YL   L+  +A +A  VTN+ G   +  L+G F+ADA LGR+ T+AT   I 
Sbjct: 47  GMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFI- 106

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLTL+ ++PG++P +C+  +          
Sbjct: 107 ------------------------YVSGMTLLTLSASVPGLKPGNCNADTCH-------P 166

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
           N  Q A+ + ALY IALG GGIK  VS F +DQFD ND  E+     FFN FYF+I++ +
Sbjct: 167 NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGA 226

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           L A  VLV++Q  VG GWG+G+    MVIAV     G+ FYR ++P GSPLT I++V++ 
Sbjct: 227 LIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVA 286

Query: 242 AWKKRSLPHPVHPRFL--------NDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           A++K S+  P     L        N   + K+ +T      DKAA+  +  +I     NP
Sbjct: 287 AFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNP 346

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + +VT+VEE+K ++ LLP+W+TGI+F  +YSQM+                   I S +
Sbjct: 347 WRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSAS 406

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S    T + ++  IP +RK T N +G T LQR+GIG+V SIF M+ AG +E  
Sbjct: 407 LSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVV 466

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +S+FW IPQ+ L+G  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 467 RLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 526

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+T+++G ++S++LVT+V  +T K     W+  NLN+G+L+YF++LLA L  LNFL +L
Sbjct: 527 SLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFLNFLVYL 563

BLAST of CmaCh02G001850 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 2.1e-89
Identity = 203/549 (36.98%), Postives = 306/549 (55.74%), Query Frame = 0

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+S NL+ YL   +++ +  ++  V+N+ G      L+G F+ADA LGR+ T+A+   I 
Sbjct: 48  GMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVI- 107

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLT++ ++PG+ P      S    H    A
Sbjct: 108 ------------------------YIAGMTLLTISASVPGLTP----TCSGETCH----A 167

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
             GQ A+ + ALY IALG GGIK  VS F +DQFD  D KE+++   FFN FYF I++ +
Sbjct: 168 TAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGA 227

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           + A  VLV++Q  VG GWG G+    M IAV+    G+ FYR +KP GSPLT + +V++ 
Sbjct: 228 MIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVA 287

Query: 242 AWKKRSLPHPVHPRFLNDYQNA--------KVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           + +K  +  P     L + Q+A        K+ +T      DKAA+  E       + + 
Sbjct: 288 SCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSS 347

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + TVT+VEE+K +++LLPIW+TGI+F ++YSQM                    I S +
Sbjct: 348 WKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSAS 407

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S      + +KL +PF+RK T + +G T LQRIGIG+V SIF MV+AG +E  
Sbjct: 408 LSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVA 467

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +++FW +PQ+FLVG  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 468 RLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 527

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNK----QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+ ++ G ++S+ LVT+V  VT       W+  NLN G+L+YF+WLLA L  LNFL +L
Sbjct: 528 SLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGLSFLNFLVYL 563

BLAST of CmaCh02G001850 vs. ExPASy TrEMBL
Match: A0A6J1G5L7 (protein NRT1/ PTR FAMILY 6.4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451111 PE=3 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 8.9e-280
Identity = 510/567 (89.95%), Postives = 524/567 (92.42%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVAT ASI
Sbjct: 50  MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATFASI 109

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAVVSSEPLRMAST+K LLYP LS FLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA
Sbjct: 110 TAVVSSEPLRMASTKKLLLYPWLSWFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 169

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISI 
Sbjct: 170 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIG 229

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRV+L
Sbjct: 230 SLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVLL 289

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHPV+PRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT
Sbjct: 290 LAWKKRSLPHPVNPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 349

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEKSWQVL------------ 360
           EVEEVKMVLKLLPIWSTGILFW IYSQ T +   +AS +  +    V+            
Sbjct: 350 EVEEVKMVLKLLPIWSTGILFWTIYSQTTTFTVEQASFMDRKVGKFVIPAGSMSAFLFIA 409

Query: 361 ----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
               TSMNEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKERR FAVGQ
Sbjct: 410 ILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRAFAVGQ 469

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFF SSL
Sbjct: 470 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFASSL 529

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLA+KHQYKDQHYSSSND
Sbjct: 530 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLARKHQYKDQHYSSSND 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELKSSS+ IA EMEGPIS+K TET
Sbjct: 590 NNEELKSSSDKIALEMEGPISIKTTET 616

BLAST of CmaCh02G001850 vs. ExPASy TrEMBL
Match: A0A6J1G5N7 (protein NRT1/ PTR FAMILY 6.4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451111 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.1e-264
Identity = 489/567 (86.24%), Postives = 502/567 (88.54%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVAT ASI
Sbjct: 50  MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATFASI 109

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCDISSTRQLHQCIA
Sbjct: 110 TAV-------------------------GVILLTLATTIPGMRPPHCDISSTRQLHQCIA 169

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISI 
Sbjct: 170 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIG 229

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRV+L
Sbjct: 230 SLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVLL 289

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHPV+PRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT
Sbjct: 290 LAWKKRSLPHPVNPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 349

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEKSWQVL------------ 360
           EVEEVKMVLKLLPIWSTGILFW IYSQ T +   +AS +  +    V+            
Sbjct: 350 EVEEVKMVLKLLPIWSTGILFWTIYSQTTTFTVEQASFMDRKVGKFVIPAGSMSAFLFIA 409

Query: 361 ----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
               TSMNEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKERR FAVGQ
Sbjct: 410 ILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRAFAVGQ 469

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFF SSL
Sbjct: 470 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFASSL 529

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLA+KHQYKDQHYSSSND
Sbjct: 530 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLARKHQYKDQHYSSSND 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELKSSS+ IA EMEGPIS+K TET
Sbjct: 590 NNEELKSSSDKIALEMEGPISIKTTET 591

BLAST of CmaCh02G001850 vs. ExPASy TrEMBL
Match: A0A6J1CGZ8 (protein NRT1/ PTR FAMILY 6.4 OS=Momordica charantia OX=3673 GN=LOC111011133 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 6.4e-246
Identity = 453/566 (80.04%), Postives = 486/566 (85.87%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHLSSAKSA IVTNFLGALNLLGLLGGFLADA+LGR+LTVA  ASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSANIVTNFLGALNLLGLLGGFLADARLGRYLTVAIFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD    RQLHQCIA
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DFRRQLHQCIA 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLA+LYAALYTIALGGGGIKSNVSGF SDQFD+NDPKEEKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLALLYAALYTIALGGGGIKSNVSGFGSDQFDINDPKEEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKR+LPHP HP FLNDYQNAKVPYT +F CLDKAAILDE  A++QNRYNPWIVSTVT
Sbjct: 291 LAWKKRNLPHPAHPSFLNDYQNAKVPYTCKFKCLDKAAILDEYTAVDQNRYNPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASI---IYGEK-------------S 360
           EVEEVKMVLKLLPIWSTGILFW IYSQMT +   +AS    I G               +
Sbjct: 351 EVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDRIVGSLEIPAGSMSAFLFIT 410

Query: 361 WQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
             ++TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VF++FGMVAAG VEKERR F+VGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFAVFGMVAAGIVEKERRAFSVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           N  IS FWLIPQ+FL+GAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 NIQISAFWLIPQYFLIGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNK+WL+SNLNKG+LNYFYWLLAVLGLLNFLFFLLLA+KHQYKDQHYS+S D
Sbjct: 531 LVTIVDKVTNKRWLRSNLNKGSLNYFYWLLAVLGLLNFLFFLLLARKHQYKDQHYSNSTD 590

Query: 541 NDEELKSSSNMIAHEMEGPISMKATE 551
           N+EELK+SSNM+A EMEGP SMKATE
Sbjct: 591 NNEELKNSSNMVAVEMEGPTSMKATE 590

BLAST of CmaCh02G001850 vs. ExPASy TrEMBL
Match: A0A5A7V3P8 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2406G00170 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.8e-241
Identity = 449/567 (79.19%), Postives = 483/567 (85.19%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ +LHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGR+LTVAT ASI
Sbjct: 51  MGISMNLVTYLVGELHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  STRQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSTRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFD NDPKEEKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HP FLNDYQNAKVPYTYRF CLDKAAI+DE A  + N  NPWIVSTVT
Sbjct: 291 LAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAIIDEYATTDPN--NPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-KSWQV------------ 360
           E+EEVKMV KL+PIWSTGILFW IYSQMT +   +AS +     S+++            
Sbjct: 351 EIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERRVGSFEIPPGSMSAFLFIA 410

Query: 361 ---LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
              +TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKER+  AVGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           +T IS FWLIPQFFLVGAGEAFAYVGQLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 STRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNK+WL+SNLNKG+L+YFYWLLAVLGLLNF FFLLLA+KHQYKDQHY SS D
Sbjct: 531 LVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNFFFFLLLARKHQYKDQHYVSSKD 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELK S++M+A EMEGPISMKA ET
Sbjct: 591 NNEELKISNDMVALEMEGPISMKAAET 589

BLAST of CmaCh02G001850 vs. ExPASy TrEMBL
Match: A0A5D3CCF7 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold757G00100 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.8e-241
Identity = 449/567 (79.19%), Postives = 483/567 (85.19%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGR+LTVAT ASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  STRQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSTRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFD NDPKEEKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HP FLNDYQNAKVPYTYRF CLDKAAI+DE A  + N  NPWIVSTVT
Sbjct: 291 LAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAIIDEYATTDPN--NPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-KSWQV------------ 360
           E+EEVKMV KL+PIWSTGILFW IYSQMT +   +AS +     S+++            
Sbjct: 351 EIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERRVGSFEIPPGSMSAFLFIA 410

Query: 361 ---LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
              +TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKER+  AVGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           +T IS FWLIPQFFLVGAGEAFAYVGQLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 STRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNK+WL+SNLNKG+L+YFYWLLAVLGLLNF FFLLLA+KHQYKDQHY SS D
Sbjct: 531 LVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNFFFFLLLARKHQYKDQHYVSSKD 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELK S++M+A E+EGPISMKA ET
Sbjct: 591 NNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CmaCh02G001850 vs. NCBI nr
Match: XP_022947156.1 (protein NRT1/ PTR FAMILY 6.4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 972.2 bits (2512), Expect = 1.8e-279
Identity = 510/567 (89.95%), Postives = 524/567 (92.42%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVAT ASI
Sbjct: 50  MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATFASI 109

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAVVSSEPLRMAST+K LLYP LS FLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA
Sbjct: 110 TAVVSSEPLRMASTKKLLLYPWLSWFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 169

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISI 
Sbjct: 170 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIG 229

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRV+L
Sbjct: 230 SLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVLL 289

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHPV+PRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT
Sbjct: 290 LAWKKRSLPHPVNPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 349

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEKSWQVL------------ 360
           EVEEVKMVLKLLPIWSTGILFW IYSQ T +   +AS +  +    V+            
Sbjct: 350 EVEEVKMVLKLLPIWSTGILFWTIYSQTTTFTVEQASFMDRKVGKFVIPAGSMSAFLFIA 409

Query: 361 ----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
               TSMNEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKERR FAVGQ
Sbjct: 410 ILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRAFAVGQ 469

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFF SSL
Sbjct: 470 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFASSL 529

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLA+KHQYKDQHYSSSND
Sbjct: 530 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLARKHQYKDQHYSSSND 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELKSSS+ IA EMEGPIS+K TET
Sbjct: 590 NNEELKSSSDKIALEMEGPISIKTTET 616

BLAST of CmaCh02G001850 vs. NCBI nr
Match: XP_023533784.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.1 bits (2387), Expect = 5.7e-265
Identity = 490/567 (86.42%), Postives = 502/567 (88.54%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVAT ASI
Sbjct: 50  MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATFASI 109

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD+SSTRQLHQCIA
Sbjct: 110 TAV-------------------------GVILLTLATTIPGMRPPHCDMSSTRQLHQCIA 169

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFDVNDPKEEKAMLFFFNRFYFAISI 
Sbjct: 170 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMLFFFNRFYFAISIG 229

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLF VIVLVYVQD VGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 230 SLFGVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 289

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT
Sbjct: 290 LAWKKRSLPHPAHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 349

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEKSWQVL------------ 360
           EVEEVKMVLKLLPIWSTGILFW IYSQMT +   +AS +  +    V+            
Sbjct: 350 EVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDRKVGKFVIPAGSMSAFLFIA 409

Query: 361 ----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
               TSMNEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKERR FAVGQ
Sbjct: 410 ILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRAFAVGQ 469

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL
Sbjct: 470 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 529

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLA+KHQYK+QHYSSSND
Sbjct: 530 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLARKHQYKNQHYSSSND 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELKSSS+ IA EMEGPISMKATET
Sbjct: 590 NNEELKSSSDKIALEMEGPISMKATET 591

BLAST of CmaCh02G001850 vs. NCBI nr
Match: XP_022947157.1 (protein NRT1/ PTR FAMILY 6.4-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 922.2 bits (2382), Expect = 2.2e-264
Identity = 489/567 (86.24%), Postives = 502/567 (88.54%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVAT ASI
Sbjct: 50  MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATFASI 109

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCDISSTRQLHQCIA
Sbjct: 110 TAV-------------------------GVILLTLATTIPGMRPPHCDISSTRQLHQCIA 169

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISI 
Sbjct: 170 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIG 229

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRV+L
Sbjct: 230 SLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVLL 289

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHPV+PRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT
Sbjct: 290 LAWKKRSLPHPVNPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 349

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEKSWQVL------------ 360
           EVEEVKMVLKLLPIWSTGILFW IYSQ T +   +AS +  +    V+            
Sbjct: 350 EVEEVKMVLKLLPIWSTGILFWTIYSQTTTFTVEQASFMDRKVGKFVIPAGSMSAFLFIA 409

Query: 361 ----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
               TSMNEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKERR FAVGQ
Sbjct: 410 ILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRAFAVGQ 469

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFF SSL
Sbjct: 470 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFASSL 529

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLA+KHQYKDQHYSSSND
Sbjct: 530 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLARKHQYKDQHYSSSND 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELKSSS+ IA EMEGPIS+K TET
Sbjct: 590 NNEELKSSSDKIALEMEGPISIKTTET 591

BLAST of CmaCh02G001850 vs. NCBI nr
Match: XP_022140467.1 (protein NRT1/ PTR FAMILY 6.4 [Momordica charantia])

HSP 1 Score: 859.8 bits (2220), Expect = 1.3e-245
Identity = 453/566 (80.04%), Postives = 486/566 (85.87%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHLSSAKSA IVTNFLGALNLLGLLGGFLADA+LGR+LTVA  ASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSANIVTNFLGALNLLGLLGGFLADARLGRYLTVAIFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD    RQLHQCIA
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DFRRQLHQCIA 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLA+LYAALYTIALGGGGIKSNVSGF SDQFD+NDPKEEKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLALLYAALYTIALGGGGIKSNVSGFGSDQFDINDPKEEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKR+LPHP HP FLNDYQNAKVPYT +F CLDKAAILDE  A++QNRYNPWIVSTVT
Sbjct: 291 LAWKKRNLPHPAHPSFLNDYQNAKVPYTCKFKCLDKAAILDEYTAVDQNRYNPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASI---IYGEK-------------S 360
           EVEEVKMVLKLLPIWSTGILFW IYSQMT +   +AS    I G               +
Sbjct: 351 EVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDRIVGSLEIPAGSMSAFLFIT 410

Query: 361 WQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
             ++TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VF++FGMVAAG VEKERR F+VGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFAVFGMVAAGIVEKERRAFSVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           N  IS FWLIPQ+FL+GAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 NIQISAFWLIPQYFLIGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNK+WL+SNLNKG+LNYFYWLLAVLGLLNFLFFLLLA+KHQYKDQHYS+S D
Sbjct: 531 LVTIVDKVTNKRWLRSNLNKGSLNYFYWLLAVLGLLNFLFFLLLARKHQYKDQHYSNSTD 590

Query: 541 NDEELKSSSNMIAHEMEGPISMKATE 551
           N+EELK+SSNM+A EMEGP SMKATE
Sbjct: 591 NNEELKNSSNMVAVEMEGPTSMKATE 590

BLAST of CmaCh02G001850 vs. NCBI nr
Match: XP_023512951.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846.3 bits (2185), Expect = 1.5e-241
Identity = 453/568 (79.75%), Postives = 485/568 (85.39%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGR+LTVAT ASI
Sbjct: 51  MGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  S RQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSRRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFDVNDPKEEKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVL+CGTTFYRFKKPRGSPLTVIWRV L
Sbjct: 231 SLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVAL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLP P HP FLNDYQNAKVPYTYRF CLDKAAILDE  A ++NR +PWIVSTVT
Sbjct: 291 LAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYVANDRNRNDPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEK--SWQV----------- 360
           EVEEVKMVLKLLPIWSTGILFW IYSQMT +   +AS + G K  S+++           
Sbjct: 351 EVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFM-GRKVGSFEIPPGSMSAFLFI 410

Query: 361 ----LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVG 420
               +TS+NEKLFIP +RKLT N QGLTSLQRIGIG+VFSIFGMVAAG VEKERR  A+G
Sbjct: 411 AILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLALG 470

Query: 421 QNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS 480
           Q TL S FWLIPQFFL+GAGEAF YVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS
Sbjct: 471 QRTL-SAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS 530

Query: 481 LLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSN 540
           LLVTIV+ VTNKQWL+SNLNKGNLNYFYWLLAVLGLLNF FFL+LA+KHQYKDQHY +S 
Sbjct: 531 LLVTIVDKVTNKQWLRSNLNKGNLNYFYWLLAVLGLLNFFFFLVLARKHQYKDQHYFNSK 590

Query: 541 DNDEELKSSSNMIAHEMEGPISMKATET 552
           DN+EELKSS++++A E+EGPIS+KATE+
Sbjct: 591 DNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of CmaCh02G001850 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 2.0e-183
Identity = 339/534 (63.48%), Postives = 397/534 (74.34%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGR+  VA SAS+
Sbjct: 51  MGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASV 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TA+                         GV+LLT+ATTI  MRPP CD    R  HQCI 
Sbjct: 111 TAL-------------------------GVLLLTVATTISSMRPPICD-DFRRLHHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANG QLA+LY ALYTIALGGGGIKSNVSGF SDQFD +DPKEEK M+FFFNRFYF+IS+ 
Sbjct: 171 ANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVI LVYVQD VGRGWGYGIS  TMV+A IVL+CGT  YRFKKP+GSP T IWRV  
Sbjct: 231 SLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGF 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRY---NPWIVS 300
           LAWKKR   +P HP  LN Y N  VP+T    CLDKAAI    ++ +   +   +PWIVS
Sbjct: 291 LAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDKAAISKNESSPSSKDFEEKDPWIVS 350

Query: 301 TVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-----------KSWQ 360
           TVT+VEEVK+V+KL+PIW+T ILFW IYSQMT +   +A+ +  +            ++ 
Sbjct: 351 TVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAFL 410

Query: 361 VL-----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFA 420
           +L     TS+NE++F+P +R+LT   QG+TSLQRIG+G+VFS+  M  A  +E  RR  A
Sbjct: 411 ILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAVAAVIENARREAA 470

Query: 421 VGQNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFV 480
           V  +  IS FWL+PQ+FLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLST+SMGFFV
Sbjct: 471 VNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTISMGFFV 530

Query: 481 SSLLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYK 516
           SSLLV++V+ VT+K WL+SNLNK  LNYFYWLL VLG LNFL F++ A KHQYK
Sbjct: 531 SSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of CmaCh02G001850 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 420.2 bits (1079), Expect = 2.5e-117
Identity = 243/551 (44.10%), Postives = 331/551 (60.07%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           +GI +NLVTYL   +HL +A +A  VTNFLG   +L LLGGF+AD  LGR+LT+A  A+I
Sbjct: 49  LGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAI 108

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A                          GV +LTL+T IPG+RPP C+ +++     C  
Sbjct: 109 QAT-------------------------GVSILTLSTIIPGLRPPRCNPTTS---SHCEQ 168

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A+G QL +LY ALY  ALG GG+K++VSGF SDQFD  +PKE   M +FFNRF+F I++ 
Sbjct: 169 ASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVG 228

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SL AV VLVYVQD VGR WGYGI    +V+A+ V + GT  YRFKK  GSP+T +  V++
Sbjct: 229 SLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIV 288

Query: 241 LAWKKRSLPHPVHPRFLNDYQN-----------AKVPYTYRFMCLDKAAILDEVAAINQN 300
            AW+ R L  P  P +L D  +            K+P+T +F  LDKAAI D+ A +  N
Sbjct: 289 AAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSN 348

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY----------------IH 360
            +N W +ST+T+VEEVK ++++LPIW+T ILFW +++Q+T                  I 
Sbjct: 349 VFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIP 408

Query: 361 SRASIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
             +  ++      + T++ +++ I   +KL     GL  LQRIG+G+ F    M  A  V
Sbjct: 409 PASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALV 468

Query: 421 EKERRGFAVGQNTLISV-----FWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMST 480
           E +R   A      +       + LIPQ+ +VG GEA  Y GQL+FF+RE P+ MK MST
Sbjct: 469 ELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMST 528

Query: 481 GLFLSTLSMGFFVSSLLVTIVENVTNK--QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 518
           GL LSTL++GFF SS+LVTIVE  T K   W+  +LNKG L  FYWL+AVL  LNFL FL
Sbjct: 529 GLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFL 571

BLAST of CmaCh02G001850 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 408.7 bits (1049), Expect = 7.5e-114
Identity = 246/543 (45.30%), Postives = 338/543 (62.25%), Query Frame = 0

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGI++NLVTYL+  +HL S+ SA IVT+F+G   LL LLGGFLAD+ LGRF T+   ++I
Sbjct: 46  MGIAVNLVTYLMETMHLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTI 105

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A+                         G   L +AT +P +RPP C          CI 
Sbjct: 106 QAL-------------------------GTGALAVATKLPELRPPTCHHGEA-----CIP 165

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A   Q+ +LY +LY IALG GG+KS++SGF SDQFD  DPKE+  M FFFNRF+F IS+ 
Sbjct: 166 ATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISMG 225

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           +L AV VLVY+QD VGR W YGI   +M IA+++ +CGT  YR+KK +GSP+  I++V+ 
Sbjct: 226 TLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVIA 285

Query: 241 LAWKKR--SLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAI---------LDEVAAINQN 300
            A++KR   LP  +   + ++ +  ++ +T +F  LDKAAI         LD VA     
Sbjct: 286 AAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLDGVAI---- 345

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRAS------------ 360
             NPW +S+VT+VEEVKM+++LLPIW+T I+FW  Y+QM  +   +AS            
Sbjct: 346 -PNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSFKIP 405

Query: 361 ----IIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
                ++   +  +  ++ ++  +PF +K      G +SLQRI IG+V S  GM AA  V
Sbjct: 406 AGSLTVFFVAAILITLAVYDRAIMPFWKKWK-GKPGFSSLQRIAIGLVLSTAGMAAAALV 465

Query: 421 EKERRGFA--VGQNTL-ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 480
           E++R   A    Q TL ISVF L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK+MSTGL
Sbjct: 466 EQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMSTGL 525

Query: 481 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 510
           FL+TLS+GFFVSS LV+IV+ VT+      WL  N+N G L+YFYWLL +L  +NF+ ++
Sbjct: 526 FLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFVVYI 552

BLAST of CmaCh02G001850 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 352.1 bits (902), Expect = 8.4e-97
Identity = 207/549 (37.70%), Postives = 314/549 (57.19%), Query Frame = 0

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+  NLV YL   L+  +A +A  VTN+ G   +  L+G F+ADA LGR+ T+AT   I 
Sbjct: 47  GMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFI- 106

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLTL+ ++PG++P +C+  +          
Sbjct: 107 ------------------------YVSGMTLLTLSASVPGLKPGNCNADTCH-------P 166

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
           N  Q A+ + ALY IALG GGIK  VS F +DQFD ND  E+     FFN FYF+I++ +
Sbjct: 167 NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGA 226

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           L A  VLV++Q  VG GWG+G+    MVIAV     G+ FYR ++P GSPLT I++V++ 
Sbjct: 227 LIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVA 286

Query: 242 AWKKRSLPHPVHPRFL--------NDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           A++K S+  P     L        N   + K+ +T      DKAA+  +  +I     NP
Sbjct: 287 AFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNP 346

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + +VT+VEE+K ++ LLP+W+TGI+F  +YSQM+                   I S +
Sbjct: 347 WRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSAS 406

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S    T + ++  IP +RK T N +G T LQR+GIG+V SIF M+ AG +E  
Sbjct: 407 LSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVV 466

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +S+FW IPQ+ L+G  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 467 RLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 526

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+T+++G ++S++LVT+V  +T K     W+  NLN+G+L+YF++LLA L  LNFL +L
Sbjct: 527 SLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFLNFLVYL 563

BLAST of CmaCh02G001850 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 331.3 bits (848), Expect = 1.5e-90
Identity = 203/549 (36.98%), Postives = 306/549 (55.74%), Query Frame = 0

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+S NL+ YL   +++ +  ++  V+N+ G      L+G F+ADA LGR+ T+A+   I 
Sbjct: 48  GMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVI- 107

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLT++ ++PG+ P      S    H    A
Sbjct: 108 ------------------------YIAGMTLLTISASVPGLTP----TCSGETCH----A 167

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
             GQ A+ + ALY IALG GGIK  VS F +DQFD  D KE+++   FFN FYF I++ +
Sbjct: 168 TAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGA 227

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           + A  VLV++Q  VG GWG G+    M IAV+    G+ FYR +KP GSPLT + +V++ 
Sbjct: 228 MIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVA 287

Query: 242 AWKKRSLPHPVHPRFLNDYQNA--------KVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           + +K  +  P     L + Q+A        K+ +T      DKAA+  E       + + 
Sbjct: 288 SCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSS 347

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + TVT+VEE+K +++LLPIW+TGI+F ++YSQM                    I S +
Sbjct: 348 WKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSAS 407

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S      + +KL +PF+RK T + +G T LQRIGIG+V SIF MV+AG +E  
Sbjct: 408 LSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVA 467

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +++FW +PQ+FLVG  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 468 RLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 527

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNK----QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+ ++ G ++S+ LVT+V  VT       W+  NLN G+L+YF+WLLA L  LNFL +L
Sbjct: 528 SLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGLSFLNFLVYL 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVE02.8e-18263.48Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q050853.5e-11644.10Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9SZY41.1e-11245.30Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q9M3901.2e-9537.70Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB82.1e-8936.98Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G5L78.9e-28089.95protein NRT1/ PTR FAMILY 6.4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1G5N71.1e-26486.24protein NRT1/ PTR FAMILY 6.4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1CGZ86.4e-24680.04protein NRT1/ PTR FAMILY 6.4 OS=Momordica charantia OX=3673 GN=LOC111011133 PE=3... [more]
A0A5A7V3P82.8e-24179.19Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A5D3CCF72.8e-24179.19Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
XP_022947156.11.8e-27989.95protein NRT1/ PTR FAMILY 6.4-like isoform X1 [Cucurbita moschata][more]
XP_023533784.15.7e-26586.42protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo][more]
XP_022947157.12.2e-26486.24protein NRT1/ PTR FAMILY 6.4-like isoform X2 [Cucurbita moschata][more]
XP_022140467.11.3e-24580.04protein NRT1/ PTR FAMILY 6.4 [Momordica charantia][more]
XP_023512951.11.5e-24179.75protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G21670.12.0e-18363.48Major facilitator superfamily protein [more]
AT1G12110.12.5e-11744.10nitrate transporter 1.1 [more]
AT2G26690.17.5e-11445.30Major facilitator superfamily protein [more]
AT3G54140.18.4e-9737.70peptide transporter 1 [more]
AT5G01180.11.5e-9036.98peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..521
e-value: 1.6E-140
score: 470.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 5..514
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 89..332
e-value: 1.8E-56
score: 191.7
coord: 350..475
e-value: 4.2E-27
score: 95.0
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..526
NoneNo IPR availablePANTHERPTHR11654:SF373PROTEIN NRT1/ PTR FAMILY 6.4-LIKEcoord: 1..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G001850.1CmaCh02G001850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity