CmaCh01G018820 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G018820
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	cytochrome P450 82C2-like
Location	Cma_Chr01: 12328508 .. 12329356 (+)
RNA-Seq Expression	CmaCh01G018820
Synteny	CmaCh01G018820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACAGCTGCCTCTCGCCGGAATAATCTTCGCCCTTCTCTTCTTTCTCTATGCTCTCTTCACTATATCCCGGAGATCCGCCGCTCATCGGAAGAAACTCCCACCGGAATCCGGTGGCGCCTGGCCGGTGATTGGCCACCTTCATTTGCTAAGTACAAAAGAGTCAACTCACATAACCCTTTCAAAATTGGCTGATAAGCACGGACCAATATTTACAATTCGGCTCGGTATGACAAAGGCCTTGATTGTGAGCAATTGGGATATAGCAAAAGAGTTTTTTACTACAAAAGACAGAATCTTTGCCTCTCGACCAAAGCTTGCAGCCTCAAAGCTATTGGGCTACGACTATGCCATGATGGGTTTGAGCCCTTACTCTCCACTTTGGCGCCATGCACGCAAGATAGCCACTCTTGAGCTCCTCACAAGAAAAGACGAAAAATTGGTGGTGGAAATGAAGAATTTGTTCGCTGACATAACGCTCAACATCATATTCATGTTGGTGATCGGAAAGCGATTCTCCACCGCCTTCAACCGCCACGACGGTGAAGATTGCCAAAAGGCATTGAGGGATTTCTTTAAATTGTTTGGAGTGTTTGTTCCATCGGATTCTTTTCAGCTTCGATGGTGACACGTCGTCAAAGCTACTTGCTTGGTAACTATTTTAGCATTGTAACTAAAACAAATTAATTTTATTAATCAACAAGTTTTGGTTCAAATATATGTAGAATATGATATTGGGTGGATTCGACACTACCACGGTGGTTTACAGAGTGTGATATTAAAAACAAAAGCTCCACTCAAAATTGTTTTATCTCCACGCCTACCTGCTCATGTTTACAGAGTGTGA

mRNA sequence

ATGGAACAGCTGCCTCTCGCCGGAATAATCTTCGCCCTTCTCTTCTTTCTCTATGCTCTCTTCACTATATCCCGGAGATCCGCCGCTCATCGGAAGAAACTCCCACCGGAATCCGGTGGCGCCTGGCCGGTGATTGGCCACCTTCATTTGCTAAGTACAAAAGAGTCAACTCACATAACCCTTTCAAAATTGGCTGATAAGCACGGACCAATATTTACAATTCGGCTCGGTATGACAAAGGCCTTGATTGTGAGCAATTGGGATATAGCAAAAGAGTTTTTTACTACAAAAGACAGAATCTTTGCCTCTCGACCAAAGCTTGCAGCCTCAAAGCTATTGGGCTACGACTATGCCATGATGGGTTTGAGCCCTTACTCTCCACTTTGGCGCCATGCACGCAAGATAGCCACTCTTGAGCTCCTCACAAGAAAAGACGAAAAATTGGTGGTGGAAATGAAGAATTTGTTCGCTGACATAACGCTCAACATCATATTCATGTTGGTGATCGGAAAGCGATTCTCCACCGCCTTCAACCGCCACGACGAATATGATATTGGGTGGATTCGACACTACCACGGTGGTTTACAGAGTGTGATATTAAAAACAAAAGCTCCACTCAAAATTGTTTTATCTCCACGCCTACCTGCTCATGTTTACAGAGTGTGA

Coding sequence (CDS)

ATGGAACAGCTGCCTCTCGCCGGAATAATCTTCGCCCTTCTCTTCTTTCTCTATGCTCTCTTCACTATATCCCGGAGATCCGCCGCTCATCGGAAGAAACTCCCACCGGAATCCGGTGGCGCCTGGCCGGTGATTGGCCACCTTCATTTGCTAAGTACAAAAGAGTCAACTCACATAACCCTTTCAAAATTGGCTGATAAGCACGGACCAATATTTACAATTCGGCTCGGTATGACAAAGGCCTTGATTGTGAGCAATTGGGATATAGCAAAAGAGTTTTTTACTACAAAAGACAGAATCTTTGCCTCTCGACCAAAGCTTGCAGCCTCAAAGCTATTGGGCTACGACTATGCCATGATGGGTTTGAGCCCTTACTCTCCACTTTGGCGCCATGCACGCAAGATAGCCACTCTTGAGCTCCTCACAAGAAAAGACGAAAAATTGGTGGTGGAAATGAAGAATTTGTTCGCTGACATAACGCTCAACATCATATTCATGTTGGTGATCGGAAAGCGATTCTCCACCGCCTTCAACCGCCACGACGAATATGATATTGGGTGGATTCGACACTACCACGGTGGTTTACAGAGTGTGATATTAAAAACAAAAGCTCCACTCAAAATTGTTTTATCTCCACGCCTACCTGCTCATGTTTACAGAGTGTGA

Protein sequence

MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPLWRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGKRFSTAFNRHDEYDIGWIRHYHGGLQSVILKTKAPLKIVLSPRLPAHVYRV

Homology

[Close]

BLAST of CmaCh01G018820 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 5.1e-34
Identity = 77/196 (39.29%), Postives = 110/196 (56.12%), Query Frame = 0


Query: 6   LAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLA 65
           L  ++      L   F    +   ++ K PP+  GAWP+IGHLHLL+  +  H  LS  A
Sbjct: 7   LETLLLGFFVLLPCFFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFA 66

Query: 66  DKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPY 125
           DK+GP+F I+LG+ +AL+V+N +IAKE FTT DR F +RP   A+K++GY+Y M+G++PY
Sbjct: 67  DKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPY 126

Query: 126 SPLWRHARKIATLELL----------------------------TRKDEKLVVEMKNLFA 174
            P WR  RKI  LE L                            T+  +  +V+MK   A
Sbjct: 127 GPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMKQWLA 186

[Close]

BLAST of CmaCh01G018820 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 1.2e-30
Identity = 76/212 (35.85%), Postives = 109/212 (51.42%), Query Frame = 0


Query: 10  IFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLADKHG 69
           + +LLFFL+      R S     K PP   G+WP++GHL L+   ++ H TL  L DK+G
Sbjct: 16  LISLLFFLF------RFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGALVDKYG 75

Query: 70  PIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPLW 129
           PIFTI+LG T AL++SNW++AKE FT  D + +SRPK  A +L+ Y+ A +G +PY   W
Sbjct: 76  PIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYW 135

Query: 130 RHARKIATLELLTRKDEKLV---------------------------------------- 173
           R  RKI TLE+L+ +  +L+                                        
Sbjct: 136 RQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSSTND 195

[Close]

BLAST of CmaCh01G018820 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.2e-30
Identity = 70/195 (35.90%), Postives = 109/195 (55.90%), Query Frame = 0


Query: 10  IFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLADKHG 69
           I ++L  ++    + R+++  R K  P   GAWP++GHL LL+  ++ H  L  LADK+G
Sbjct: 15  IASILSLIFLCLFLYRKNS--RGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALADKYG 74

Query: 70  PIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPLW 129
           P+FTI+LGM  AL++SNW+++KE FTT D   +SRPKL A +++ Y+ A +GL+PY P W
Sbjct: 75  PLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYW 134

Query: 130 RHARKIATLELLTRK-------------------------------DEKLVVEMKNLFAD 174
           R  RKI T E L+ +                                   +V++K   A 
Sbjct: 135 RELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAY 194

[Close]

BLAST of CmaCh01G018820 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.4e-30
Identity = 74/201 (36.82%), Postives = 106/201 (52.74%), Query Frame = 0


Query: 6   LAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLA 65
           +  +IF L  FLY       +      K  P  GGAWP+ GHL LL   +S H  L  LA
Sbjct: 15  VVSLIFLLCLFLYG----PLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALA 74

Query: 66  DKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPY 125
           +KHGP+FTI+LG  KAL+VS+W++A+E FTT D   ++RPKL  ++L+ Y+ AM+ ++PY
Sbjct: 75  EKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPY 134

Query: 126 SPLWRHARKIATLELLT------------------------------RKDEKLVVEMKNL 177
            P WR  RKI   E+L+                               + +   VE+K  
Sbjct: 135 GPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQW 194

[Close]

BLAST of CmaCh01G018820 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.9e-30
Identity = 72/191 (37.70%), Postives = 110/191 (57.59%), Query Frame = 0


Query: 13  LLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKES-THITLSKLADKHGPI 72
           L+F   ALF   ++S   +    P   GAWP+IGHLHLL  KE   + TL K+AD +GP 
Sbjct: 13  LVFVFIALF---KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPA 72

Query: 73  FTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPLWRH 132
            +++LG  +A +VS++++AK+ FT  D+  ASRP  AA+K +GY++A+ G +PYS  WR 
Sbjct: 73  MSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWRE 132

Query: 133 ARKIATLELLTRK-----------------------------DEKLVVEMKNLFADITLN 174
            RKIAT+ELL+ +                              + ++V++K+   D+TLN
Sbjct: 133 MRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLN 192

[Close]

BLAST of CmaCh01G018820 vs. ExPASy TrEMBL
Match: A0A6J1J289 (cytochrome P450 82C2-like OS=Cucurbita maxima OX=3661 GN=LOC111481163 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.7e-96
Identity = 182/184 (98.91%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60
           MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT
Sbjct: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60

Query: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120
           LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM
Sbjct: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120

Query: 121 GLSPYSPLWRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGKRFSTAFNRH 180
           GLSPYSPLWRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGKRFSTAFNRH
Sbjct: 121 GLSPYSPLWRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGKRFSTAFNRH 180

Query: 181 DEYD 185
           D  D
Sbjct: 181 DGED 184

[Close]

BLAST of CmaCh01G018820 vs. ExPASy TrEMBL
Match: A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.3e-83
Identity = 168/212 (79.25%), Postives = 172/212 (81.13%), Query Frame = 0


Query: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60
           MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPE GGAWPVIGHLHLLSTKE THIT
Sbjct: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPECGGAWPVIGHLHLLSTKEPTHIT 60

Query: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120
           LSKLADK+GPIFT R GM KALIVSNW+IAKE FTTKDRIFASRPKLAAS LLGYDYAMM
Sbjct: 61  LSKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMM 120

Query: 121 GLSPYSPLWRHARKIATLELLT----------------------------RKDEKLVVEM 180
           GLSPYSP WRHARKIATLELLT                            RKDEKLVVEM
Sbjct: 121 GLSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEM 180

Query: 181 KNLFADITLNIIFMLVIGKRFSTAFNRHDEYD 185
           KNLFADITLN IFM+VIGKRFSTAF+ HD  D
Sbjct: 181 KNLFADITLNTIFMMVIGKRFSTAFDGHDGED 212

[Close]

BLAST of CmaCh01G018820 vs. ExPASy TrEMBL
Match: A0A6J1FPG1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.3e-83
Identity = 168/212 (79.25%), Postives = 172/212 (81.13%), Query Frame = 0


Query: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60
           MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPE GGAWPVIGHLHLLSTKE THIT
Sbjct: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPECGGAWPVIGHLHLLSTKEPTHIT 60

Query: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120
           LSKLADK+GPIFT R GM KALIVSNW+IAKE FTTKDRIFASRPKLAAS LLGYDYAMM
Sbjct: 61  LSKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMM 120

Query: 121 GLSPYSPLWRHARKIATLELLT----------------------------RKDEKLVVEM 180
           GLSPYSP WRHARKIATLELLT                            RKDEKLVVEM
Sbjct: 121 GLSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEM 180

Query: 181 KNLFADITLNIIFMLVIGKRFSTAFNRHDEYD 185
           KNLFADITLN IFM+VIGKRFSTAF+ HD  D
Sbjct: 181 KNLFADITLNTIFMMVIGKRFSTAFDGHDGED 212

[Close]

BLAST of CmaCh01G018820 vs. ExPASy TrEMBL
Match: A0A6J1CCZ4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 7.9e-62
Identity = 125/204 (61.27%), Postives = 150/204 (73.53%), Query Frame = 0


Query: 4   LPLAGIIFALLFFLYALFTISRRSAAHRKKL--PPESGGAWPVIGHLHLLSTKESTHITL 63
           LP+   +FALL FLYALFT+SR+S+A R++L  PPE+GGAWPVIGHLH LS KE THITL
Sbjct: 8   LPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITL 67

Query: 64  SKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMG 123
           +K+AD +GP+FT+R GM KAL+VS+W+IA+EFFTT DR+FASRPKL ASKLLGYDYAM G
Sbjct: 68  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 127

Query: 124 LSPYSPLWRHARKIATLELLT---------------------------RKDEKLVVEMKN 179
           LSPY P WR AR++ATLELLT                             +EK VVEMK 
Sbjct: 128 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEKAVVEMKT 187

[Close]

BLAST of CmaCh01G018820 vs. ExPASy TrEMBL
Match: A0A5A7SQD8 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00980 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.5e-60
Identity = 126/199 (63.32%), Postives = 140/199 (70.35%), Query Frame = 0


Query: 6   LAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLA 65
           L GIIF LL   Y LFT SRRS A RK+LPP++GGAWPVIGHLHLL+  E THIT +K+A
Sbjct: 7   LFGIIFPLLILFYVLFTWSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHITFAKMA 66

Query: 66  DKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPY 125
           D +GP+FT R GM +ALIVSNW++AKEFFTT DRIFASRPKL ASKLL YDYAMMG SPY
Sbjct: 67  DAYGPMFTFRFGMNEALIVSNWELAKEFFTTNDRIFASRPKLVASKLLAYDYAMMGFSPY 126

Query: 126 SPLWRHARKIATLELLT-----------------------------RKDEKLVVEMKNLF 176
           SP WRH RKIATLELLT                              K EK+VVEMK   
Sbjct: 127 SPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVVEMKKRL 186

[Close]

BLAST of CmaCh01G018820 vs. NCBI nr
Match: XP_022982285.1 (cytochrome P450 82C2-like [Cucurbita maxima])

HSP 1 Score: 362.1 bits (928), Expect = 3.5e-96
Identity = 182/184 (98.91%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60
           MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT
Sbjct: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60

Query: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120
           LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM
Sbjct: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120

Query: 121 GLSPYSPLWRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGKRFSTAFNRH 180
           GLSPYSPLWRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGKRFSTAFNRH
Sbjct: 121 GLSPYSPLWRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGKRFSTAFNRH 180

Query: 181 DEYD 185
           D  D
Sbjct: 181 DGED 184

[Close]

BLAST of CmaCh01G018820 vs. NCBI nr
Match: XP_022940488.1 (cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 319.3 bits (817), Expect = 2.6e-83
Identity = 168/212 (79.25%), Postives = 172/212 (81.13%), Query Frame = 0


Query: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60
           MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPE GGAWPVIGHLHLLSTKE THIT
Sbjct: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPECGGAWPVIGHLHLLSTKEPTHIT 60

Query: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120
           LSKLADK+GPIFT R GM KALIVSNW+IAKE FTTKDRIFASRPKLAAS LLGYDYAMM
Sbjct: 61  LSKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMM 120

Query: 121 GLSPYSPLWRHARKIATLELLT----------------------------RKDEKLVVEM 180
           GLSPYSP WRHARKIATLELLT                            RKDEKLVVEM
Sbjct: 121 GLSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEM 180

Query: 181 KNLFADITLNIIFMLVIGKRFSTAFNRHDEYD 185
           KNLFADITLN IFM+VIGKRFSTAF+ HD  D
Sbjct: 181 KNLFADITLNTIFMMVIGKRFSTAFDGHDGED 212

[Close]

BLAST of CmaCh01G018820 vs. NCBI nr
Match: XP_022940485.1 (cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 319.3 bits (817), Expect = 2.6e-83
Identity = 168/212 (79.25%), Postives = 172/212 (81.13%), Query Frame = 0


Query: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHIT 60
           MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPE GGAWPVIGHLHLLSTKE THIT
Sbjct: 1   MEQLPLAGIIFALLFFLYALFTISRRSAAHRKKLPPECGGAWPVIGHLHLLSTKEPTHIT 60

Query: 61  LSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMM 120
           LSKLADK+GPIFT R GM KALIVSNW+IAKE FTTKDRIFASRPKLAAS LLGYDYAMM
Sbjct: 61  LSKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMM 120

Query: 121 GLSPYSPLWRHARKIATLELLT----------------------------RKDEKLVVEM 180
           GLSPYSP WRHARKIATLELLT                            RKDEKLVVEM
Sbjct: 121 GLSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEM 180

Query: 181 KNLFADITLNIIFMLVIGKRFSTAFNRHDEYD 185
           KNLFADITLN IFM+VIGKRFSTAF+ HD  D
Sbjct: 181 KNLFADITLNTIFMMVIGKRFSTAFDGHDGED 212

[Close]

BLAST of CmaCh01G018820 vs. NCBI nr
Match: XP_022138438.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 246.9 bits (629), Expect = 1.6e-61
Identity = 125/204 (61.27%), Postives = 150/204 (73.53%), Query Frame = 0


Query: 4   LPLAGIIFALLFFLYALFTISRRSAAHRKKL--PPESGGAWPVIGHLHLLSTKESTHITL 63
           LP+   +FALL FLYALFT+SR+S+A R++L  PPE+GGAWPVIGHLH LS KE THITL
Sbjct: 8   LPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITL 67

Query: 64  SKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMG 123
           +K+AD +GP+FT+R GM KAL+VS+W+IA+EFFTT DR+FASRPKL ASKLLGYDYAM G
Sbjct: 68  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 127

Query: 124 LSPYSPLWRHARKIATLELLT---------------------------RKDEKLVVEMKN 179
           LSPY P WR AR++ATLELLT                             +EK VVEMK 
Sbjct: 128 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEKAVVEMKT 187

[Close]

BLAST of CmaCh01G018820 vs. NCBI nr
Match: XP_023535449.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246.1 bits (627), Expect = 2.8e-61
Identity = 130/214 (60.75%), Postives = 147/214 (68.69%), Query Frame = 0


Query: 1   MEQL-PLAGIIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHI 60
           ME L PLAGIIF  L FLY  FT SRRS AHRK++PPE+GGAWPVIGHLHLL   E THI
Sbjct: 1   MEHLRPLAGIIFPFLLFLYVFFTWSRRSVAHRKRIPPEAGGAWPVIGHLHLLHAGEPTHI 60

Query: 61  TLSKLADKHGPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAM 120
           TL+K+AD +GPIFT R GM KALIVS+W+IAKEFFTT DRIFASRPKL ASKLL YDYAM
Sbjct: 61  TLAKMADAYGPIFTFRFGMKKALIVSSWEIAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGLSPYSPLWRHARKIATLELLT------------------------------RKDEKLV 180
           +G SPY+P WR  RKIATLELLT                               + EK+ 
Sbjct: 121 IGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQQWMNELHKMSIKSNERGEKVA 180

Query: 181 VEMKNLFADITLNIIFMLVIGKRFSTAFNRHDEY 184
           VEMK   ADITLN IF +VIGKRFS   + ++ +
Sbjct: 181 VEMKKWLADITLNTIFKMVIGKRFSAIQHGNENF 214

[Close]

BLAST of CmaCh01G018820 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 132.5 bits (332), Expect = 4.2e-31
Identity = 72/191 (37.70%), Postives = 110/191 (57.59%), Query Frame = 0


Query: 13  LLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKES-THITLSKLADKHGPI 72
           L+F   ALF   ++S   +    P   GAWP+IGHLHLL  KE   + TL K+AD +GP 
Sbjct: 13  LVFVFIALF---KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPA 72

Query: 73  FTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPLWRH 132
            +++LG  +A +VS++++AK+ FT  D+  ASRP  AA+K +GY++A+ G +PYS  WR 
Sbjct: 73  MSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWRE 132

Query: 133 ARKIATLELLTRK-----------------------------DEKLVVEMKNLFADITLN 174
            RKIAT+ELL+ +                              + ++V++K+   D+TLN
Sbjct: 133 MRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLN 192

[Close]

BLAST of CmaCh01G018820 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 128.3 bits (321), Expect = 7.9e-30
Identity = 66/160 (41.25%), Postives = 102/160 (63.75%), Query Frame = 0


Query: 13  LLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKES-THITLSKLADKHGPI 72
           L+F   ALF   ++S   +    P   GAWP+IGHLHLLS KE   + TL K+AD++GP 
Sbjct: 13  LVFVFIALF---KKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPA 72

Query: 73  FTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPLWRH 132
            ++RLG ++  +VS++++AK+ FT  D+  ASRP  AA+K +GYD A+ G +PYS  WR 
Sbjct: 73  MSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWRE 132

Query: 133 ARKIATLELLTRKDEKLVVEMKNLFADITLNIIFMLVIGK 172
            RKIATLELL+ +  +++  ++     + +  ++ L + K
Sbjct: 133 MRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKK 169

[Close]

BLAST of CmaCh01G018820 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 116.3 bits (290), Expect = 3.1e-26
Identity = 57/158 (36.08%), Postives = 90/158 (56.96%), Query Frame = 0


Query: 9   IIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLADKH 68
           ++ AL  F   +   SR    ++K   P   GAWP++GHLHL  T   TH+T   +AD +
Sbjct: 8   LLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVY 67

Query: 69  GPIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPL 128
           GP+F  +LG  K +I+++ ++AKE +T  D++   RP+L ASKLLGY+ + +  SPY   
Sbjct: 68  GPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYGLY 127

Query: 129 WRHARKIATLELLTRKDEKLVVEMKNLFADITLNIIFM 167
           WR  RKIA  EL +     + +  +   AD+    +++
Sbjct: 128 WREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYV 164

[Close]

BLAST of CmaCh01G018820 vs. TAIR 10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7 )

HSP 1 Score: 105.1 bits (261), Expect = 7.1e-23
Identity = 70/198 (35.35%), Postives = 97/198 (48.99%), Query Frame = 0


Query: 12  ALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLADKHG-- 71
           A LFF+       +R +  +  LPP      P IGHLHLL  K+  H T    +   G  
Sbjct: 55  AFLFFISLKLLFGKRHS--KFNLPPSPARPLPFIGHLHLL--KQPLHRTFLSFSQSLGDA 114

Query: 72  PIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYSPLW 131
           PIF++RLG    ++VS++ IA+E FT  D + A+RPK    K + Y++  M  +PY   W
Sbjct: 115 PIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSAPYGDHW 174

Query: 132 RHARKIATLELLT----------RKDE---------------KLVVEMKNLFADITLNII 183
           R+ R+I TLE+ +          RKDE                  VEM+ LF D+T+N I
Sbjct: 175 RNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYDLTINNI 234

[Close]

BLAST of CmaCh01G018820 vs. TAIR 10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6 )

HSP 1 Score: 104.8 bits (260), Expect = 9.3e-23
Identity = 71/201 (35.32%), Postives = 102/201 (50.75%), Query Frame = 0


Query: 9   IIFALLFFLYALFTISRRSAAHRKKLPPESGGAWPVIGHLHLLSTKESTHITLSKLADKH 68
           ++ +L FFL+    +       +  LPP      P+IGHLHLL  K   H T    +   
Sbjct: 21  LLLSLAFFLFISVKLLFGKRLSKFNLPPSPARPLPLIGHLHLL--KLPLHRTFLSFSQSL 80

Query: 69  G--PIFTIRLGMTKALIVSNWDIAKEFFTTKDRIFASRPKLAASKLLGYDYAMMGLSPYS 128
           G  PIF +RLG    ++VS++ IA+E FT  D +FA+RP+L   K + Y+   M  +PY 
Sbjct: 81  GGAPIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYG 140

Query: 129 PLWRHARKIATLE----------LLTRKDE--KLV-------------VEMKNLFADITL 183
             WR+ R+IATLE          L  RKDE  +L+             VEM++L  ++T+
Sbjct: 141 DHWRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFELTI 200

The following BLAST results are available for this feature:

BLAST of CmaCh01G018820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0N7F297	5.1e-34	39.29	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
Q43068	1.2e-30	35.85	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
O49858	1.2e-30	35.90	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
O49859	3.4e-30	36.82	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
Q9SZ46	5.9e-30	37.70	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]

BLAST of CmaCh01G018820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J289	1.7e-96	98.91	cytochrome P450 82C2-like OS=Cucurbita maxima OX=3661 GN=LOC111481163 PE=3 SV=1	[more]
A0A6J1FQP6	1.3e-83	79.25	cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1FPG1	1.3e-83	79.25	cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1CCZ4	7.9e-62	61.27	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=...	[more]
A0A5A7SQD8	1.5e-60	63.32	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of CmaCh01G018820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982285.1	3.5e-96	98.91	cytochrome P450 82C2-like [Cucurbita maxima]	[more]
XP_022940488.1	2.6e-83	79.25	cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata]	[more]
XP_022940485.1	2.6e-83	79.25	cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata]	[more]
XP_022138438.1	1.6e-61	61.27	cytochrome P450 CYP82D47-like [Momordica charantia]	[more]
XP_023535449.1	2.8e-61	60.75	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh01G018820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	4.2e-31	37.70	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	7.9e-30	41.25	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT2G25160.1	3.1e-26	36.08	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT2G23190.1	7.1e-23	35.35	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]
AT2G23220.1	9.3e-23	35.32	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 89..110 score: 25.28 coord: 65..84 score: 30.12
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 36..144 e-value: 1.2E-12 score: 47.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 19..217 e-value: 1.2E-28 score: 101.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..192
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 144..178 coord: 32..143
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 144..178
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 32..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G018820.1	CmaCh01G018820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen