CmaCh01G018140 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G018140
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein DCL homolog, chloroplastic-like
Location	Cma_Chr01: 12051206 .. 12054224 (-)
RNA-Seq Expression	CmaCh01G018140
Synteny	CmaCh01G018140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTCTACTTGCTTCATGCTCACCGCAGAGCTGCGCTCCACATGAACATTTGATCATCATGGTTGCTTCTTTGCTGCTCAGGGGGCATCCTCTCATTCGGTTAGGGCTCAGGAACCGCGGGCTATGTACTGGGATCCTGCAGGTGACTCGCCGGTCTTGCTGCACTGCGATGCCGGCGTCTACTCCACCGGGCGGCGGCTTATCATCTGCTGAAAATACAACCTCTGTACTGAGTACCACTGACCCACCGAAGTACCAAAGGTGGGATGAGCCTGGTTATAGAAAGTGGAAGAATCAGGAAGAGGAAATTCTCAGCGATATCGACCCTATCATATCCCTCACAAAGGAGATCCTCCACTCCAATAGGTTTGTCCTGTTCGCGTAATTTCACCCCGAAAGTTTGTACTTTATGTTATGGGCCTTATGTGTTTGCTTTAACTTTTTAATAAATGATCACAGTTATTAGGATTTCCTTATTCCCAGTTTTACTCAATGTGATATGTTATATAGGTTTTTTTTTTTCCTTTTTTGTTAGTATTACAAATTTGAATTATGAGTATGTAAACTTATGTACTATTGAGTTATTCTCACTTTTGACCCATAATTTCTGTATTCATAAAAATATGATAACCGCTTTCAAATTATTCAATGAGGTCGGCACAATCTAGCTGCCTCTTAATAACCGTGGATGTAGACCTAAAACTAGACATGGATACAAAAGTAAAAGGGTTTTACAATCTCTTTCGCACTTCTAATGGTGTTAAACTGGCAATCATATTGTAGAGGCTTCCTCACCAACTACGGGTAAGCTTTCAAAGGCATTATAGTAGTAAAAAGAAATCAATAATGGAATTGGGTTTAATGTCAGTATGTTGTATGTGCCCTTTCTTAATTTTCTTCATGGATTTGGGATTTGATTTTCTGCTTCTTCTGCAAAGAACTGCCTTTGAGTGTAATATATACCTTAAGGGGAGCATACCTCTTGCAAGGTTGGCATGTTCATGAGCTGGTCTCATTTGGCACTTTTCCATATGATATATTGCTCAAGTTTTCGTTTTACTATCACATTTTTCTCCTAAGATATTATCTAGCCTTCTCTTAAGAACCTCTCTTTTGTATACTAGGTATGTCGATGGGGAGCGATTGACATTTGAGGATGAGAAAATTGTGGTTGACAGGCTTCTAGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGACTTGAATCCATTATGGTACGCTTTGATATAGACTAAATGATTTATATACTTCTTACTATGTTTCAAACATGCAGTCGCTTGCTTTTGTTTGTTAGGCCGTATAGAAGTAAAAGCAGGTAAAGGAACAAATAACGTTTGGTATATGATATTCTCAAAATCTCAAAATACCATCTCTTACCTATTCTTTAAAGCAAAGCCTGTTCAAGAATTGGTTTCTAACTAATTTACCCTGGCGTTGTAGGCCTTGTGTGATAGGACCTCAGAAAGTAGTTTCTTCTTTGCCACTTTTTTTTTGTTGGGGGAGGAAGATCTCATGAAGTTGCTTGACTGGAAGTCTAAACATTGCTGCAGTTGCGTGGACATAGTGGTTGTGAGCTCCGAGAACCTTATGAAATGAATTCTAAACGGGAAAAATTGAAGAATTCTAAAAGTATGCCCTACCAATCATTAGCTAAACGTGGCACAAAGATAAAAGTTCGACTTTGTTCAATAGCTACAGTAAATAGAATGGTTCGAATTGGCAGAAGCTTCTGGTTTCACACCTCAATCTTTACCAGAATACTTGCCTACCTTGACTACCAATCTAACAAACTTCCTAGGTATTGAGTAATTTACCCCTGATAACATTTGTAGCACGCCTCCTTAAGTTTCTGCCCGTGTGGTCATGTAATGGCCTCCAATTGTCACTCAACTCCTTCCCAATAAGAGGTAGATTTGAACATGCTTGAAGTTTGCAAGGACTGGGAATCAACCTGTATTTTGTGTGCTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAGGAATGTGGAGATAGATTGATGGCCCTCTTGAACTAATCATTGGGAGACGAGTTGTTTGTCATATTTCTTTGTGAATTTTGTGAATAGAAAAGGGATTCCTAGTACACTTTTGATATCAACAAAGCTAACTTGTATTAATTATTTAAGCTATTTTATGTGGTTGAAGGTCGATCGGCACCCCCAATTTCGGCACTCCAGATGCCTCTTTGTCATAAGGACCGATGGTGGATGGATTGATTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAATACCCATTATATGCAGACCGGTTTATTCGGGAACATTTCAAACGTGGTAGTTGATGAATTTATGCTAGCCTTGATGTTAGCCTGTGGATATTTGTTCTGGTTAAGAGTTACATATGAATGATTAAATGTATTCTTGATGAGTCAGTGGCCTACGTGAAGGCAAGATGTACAGATTGTCAATGCAGTAGCTATCATATCTTGGAAGTTCTGCACAATTTTGAAAATGAGAACAATCGAGAATCATAGGAGTGAACCTTCTCTTTGTTGACTTGCAGCTGCTCTGTTTAGTTTGTGGTTAAGATTACTGTATAATCAGATCACCAGGACATGTAGAACGAGATTATTGATAATGTTTGAAAGTCGTATAGGATTGAAAAGTTTGAACTGGGTTTGATTGATAATCTATTCTCTACACTTCTTTCCACGTGCTAG

mRNA sequence

CGTCTACTTGCTTCATGCTCACCGCAGAGCTGCGCTCCACATGAACATTTGATCATCATGGTTGCTTCTTTGCTGCTCAGGGGGCATCCTCTCATTCGGTTAGGGCTCAGGAACCGCGGGCTATGTACTGGGATCCTGCAGGTGACTCGCCGGTCTTGCTGCACTGCGATGCCGGCGTCTACTCCACCGGGCGGCGGCTTATCATCTGCTGAAAATACAACCTCTGTACTGAGTACCACTGACCCACCGAAGTACCAAAGGTGGGATGAGCCTGGTTATAGAAAGTGGAAGAATCAGGAAGAGGAAATTCTCAGCGATATCGACCCTATCATATCCCTCACAAAGGAGATCCTCCACTCCAATAGGTATGTCGATGGGGAGCGATTGACATTTGAGGATGAGAAAATTGTGGTTGACAGGCTTCTAGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGACTTGAATCCATTATGGTCGATCGGCACCCCCAATTTCGGCACTCCAGATGCCTCTTTGTCATAAGGACCGATGGTGGATGGATTGATTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAATACCCATTATATGCAGACCGGTTTATTCGGGAACATTTCAAACGTGGTAGTTGATGAATTTATGCTAGCCTTGATGTTAGCCTGTGGATATTTGTTCTGGTTAAGAGTTACATATGAATGATTAAATGTATTCTTGATGAGTCAGTGGCCTACGTGAAGGCAAGATGTACAGATTGTCAATGCAGTAGCTATCATATCTTGGAAGTTCTGCACAATTTTGAAAATGAGAACAATCGAGAATCATAGGAGTGAACCTTCTCTTTGTTGACTTGCAGCTGCTCTGTTTAGTTTGTGGTTAAGATTACTGTATAATCAGATCACCAGGACATGTAGAACGAGATTATTGATAATGTTTGAAAGTCGTATAGGATTGAAAAGTTTGAACTGGGTTTGATTGATAATCTATTCTCTACACTTCTTTCCACGTGCTAG

Coding sequence (CDS)

ATGGTTGCTTCTTTGCTGCTCAGGGGGCATCCTCTCATTCGGTTAGGGCTCAGGAACCGCGGGCTATGTACTGGGATCCTGCAGGTGACTCGCCGGTCTTGCTGCACTGCGATGCCGGCGTCTACTCCACCGGGCGGCGGCTTATCATCTGCTGAAAATACAACCTCTGTACTGAGTACCACTGACCCACCGAAGTACCAAAGGTGGGATGAGCCTGGTTATAGAAAGTGGAAGAATCAGGAAGAGGAAATTCTCAGCGATATCGACCCTATCATATCCCTCACAAAGGAGATCCTCCACTCCAATAGGTATGTCGATGGGGAGCGATTGACATTTGAGGATGAGAAAATTGTGGTTGACAGGCTTCTAGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGACTTGAATCCATTATGGTCGATCGGCACCCCCAATTTCGGCACTCCAGATGCCTCTTTGTCATAAGGACCGATGGTGGATGGATTGATTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAATACCCATTATATGCAGACCGGTTTATTCGGGAACATTTCAAACGTGGTAGTTGA

Protein sequence

MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSTTDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKRGS

Homology

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BLAST of CmaCh01G018140 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.5e-29
Identity = 54/114 (47.37%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 81  EEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESI 140
           E++IL    P++   + ILHS +Y + +RL+ E E+ +++ LL +HP  E KIGCG++ I
Sbjct: 101 EDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYI 160

Query: 141 MVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKR 195
           MV  HP F  SRC+F++R DG  +DFSY KC++  I+ KYPLYAD FI  HF++
Sbjct: 161 MVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of CmaCh01G018140 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.7e-28
Identity = 67/179 (37.43%), Postives = 102/179 (56.98%), Query Frame = 0


Query: 32  RSCCTAMPASTPPGGGLSSAEN--------TTSVLSTTDPP---KYQRWDEPGYRK---- 91
           R C  A   +   GGG+ S           +T + +T++     K +  DE G +     
Sbjct: 41  RLCRCAAVKTGSEGGGIRSDNAELLRKPVISTELETTSESEELVKEESDDEVGKKSGDGE 100

Query: 92  -WKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGC 151
            W + E++IL D  P++   + ILHS +Y  G+RL+ + ++ ++ RLL +HP  + KIG 
Sbjct: 101 GWVDWEDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKKIGP 160

Query: 152 GLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKR 195
           G++ I V  HP F +SRCLF++R DG  +DFSY KC++  IR  YPLYAD FI  HF++
Sbjct: 161 GVDYITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHFRK 219

[Close]

BLAST of CmaCh01G018140 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 5.6e-24
Identity = 47/116 (40.52%), Postives = 79/116 (68.10%), Query Frame = 0


Query: 79   NQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLE 138
            ++E+E+LSD++P++   ++I+H + Y DG+ ++ +D+  V++++L  HP  E K+G G++
Sbjct: 1734 SEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVD 1793

Query: 139  SIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKR 195
             I VD+H  F  SRC FV+ TDG   DFSY+K L  Y+  KYP  A+ FI ++F +
Sbjct: 1794 FITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of CmaCh01G018140 vs. ExPASy Swiss-Prot
Match: Q9LQ02 (DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD1 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.1e-06
Identity = 39/120 (32.50%), Postives = 59/120 (49.17%), Query Frame = 0


Query: 77   WKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCG 136
            WKN        I+ +    K ILHS  Y   E L   DE + V  +L  HP++ +KIG G
Sbjct: 1336 WKN--------IELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPG 1395

Query: 137  LESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKRGS 196
            ++ I V +  +   S C  V+R DG + DFSY KC+    +   P   + +  ++ K G+
Sbjct: 1396 VKGIRVAK-SKHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1443

[Close]

BLAST of CmaCh01G018140 vs. ExPASy TrEMBL
Match: A0A6J1J4G4 (protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481165 PE=4 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 3.7e-111
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           PLYADRFIREHFKRGS
Sbjct: 181 PLYADRFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. ExPASy TrEMBL
Match: A0A6J1FNQ4 (protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445919 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 3.8e-108
Identity = 190/196 (96.94%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           MVASLLLRGHPLIRLGLRNRGLC GI+QVTRRSCCTA+PASTPPGGGLSSAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRW+EPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           PLYA+RFIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. ExPASy TrEMBL
Match: A0A6J1C4J4 (protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007322 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.7e-97
Identity = 171/196 (87.24%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           M ASLLLRGHPL+RLGLR+RGLCTGI+QVTRRSCCTA  A TPP G L+SAEN TSVLS+
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           +DPPKY RWDEP YRKWK+QEEE+L+DI+PIISLTKEILHSNRYVDGERLT  DE+IVV+
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           P YA+RFIREHFKRGS
Sbjct: 181 PSYAERFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. ExPASy TrEMBL
Match: A0A1S3BU88 (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.5e-92
Identity = 159/196 (81.12%), Postives = 177/196 (90.31%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           M AS L RGHPL+RLGL+ RGLCTG++QVTRRSCC+A  ASTP  G L++ +N TS++S+
Sbjct: 1   MAASFLFRGHPLLRLGLKRRGLCTGVVQVTRRSCCSATVASTPSDGDLTAGDNATSLVSS 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           ++PPKYQRWDEP YRKWKNQEEEIL DI+PII LTKEILHS+RY DGERLT EDE+ VVD
Sbjct: 61  SEPPKYQRWDEPDYRKWKNQEEEILGDIEPIIFLTKEILHSHRYADGERLTLEDERTVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRC FVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCFFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           P +A+RFIREHFKRGS
Sbjct: 181 PSFAERFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. ExPASy TrEMBL
Match: A0A6J1IHJ1 (protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477278 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 3.6e-90
Identity = 159/196 (81.12%), Postives = 177/196 (90.31%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           M ASLLLRGHPL+R GL +RGLC+G++QVTRRSCCTA  ASTPP G L+SAENT SV S 
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           +   KYQRW EP YRKWK+QE+EILSD++P++SLTKEILHSNRYVDGERLT EDE+IVVD
Sbjct: 61  SQSTKYQRWCEPDYRKWKDQEDEILSDVEPVMSLTKEILHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFR+SRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRYSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PLYADRFIREHFKRGS 197
           P YA+ FIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. NCBI nr
Match: XP_022982288.1 (protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 410.6 bits (1054), Expect = 7.6e-111
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           PLYADRFIREHFKRGS
Sbjct: 181 PLYADRFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. NCBI nr
Match: KAG6608449.1 (Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 405.6 bits (1041), Expect = 2.4e-109
Identity = 192/196 (97.96%), Postives = 195/196 (99.49%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           MVASLLLRGHPLIRLGLRNRGLCTGI+QVTRRSCCTAMPASTPPGGGLSSAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDP+ISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           PLYA+RFIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. NCBI nr
Match: KAG7037785.1 (Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 401.4 bits (1030), Expect = 4.6e-108
Identity = 190/194 (97.94%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           MVASLLLRGHPLIRLGLRNRGLCTGI+QVTRRSCCTAMPASTPPGGGLSSAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDP+ISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKR 195
           PLYA+RFIREHFKR
Sbjct: 181 PLYAERFIREHFKR 194

[Close]

BLAST of CmaCh01G018140 vs. NCBI nr
Match: XP_022940238.1 (protein DCL homolog, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 400.6 bits (1028), Expect = 7.8e-108
Identity = 190/196 (96.94%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           MVASLLLRGHPLIRLGLRNRGLC GI+QVTRRSCCTA+PASTPPGGGLSSAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRW+EPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           PLYA+RFIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. NCBI nr
Match: XP_023523235.1 (protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 400.2 bits (1027), Expect = 1.0e-107
Identity = 187/196 (95.41%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60
           MVASLLLRGHPLIRLGLRNRGLCTG+++VTRRSCCTAMPASTPPGGGLSSAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGVVRVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPP YQRWDEPGYRKWKNQEEEILSDIDP+ISLTKEILHSNRYVDGERLTFEDEKI+VD
Sbjct: 61  TDPPNYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIMVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFV+RTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYADRFIREHFKRGS 197
           PLYA+RFIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of CmaCh01G018140 vs. TAIR 10
Match: AT3G46630.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 231.5 bits (589), Expect = 5.9e-61
Identity = 120/210 (57.14%), Postives = 148/210 (70.48%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRG-----LCTGILQVTRRSCCTAM---------PASTPPGG 60
           M + LLLR  PL+R G  N       +  GIL   RR  C+            + +P  G
Sbjct: 1   MTSLLLLRTLPLLRNGFLNHRHQVGIVAGGILSHRRRLLCSLADRPQFYENNDSVSPVEG 60

Query: 61  GLSSAENTTSVLSTTDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVD 120
             ++A N TS +         R+++P YRKWKN E EIL DI+PI  L KEILHS+RY+D
Sbjct: 61  SGAAAMNVTSPVVDNS----WRYEDPDYRKWKNLEAEILRDIEPISLLAKEILHSDRYLD 120

Query: 121 GERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDF 180
           GERL FEDEKIV+++LL +HP+++DKIGCGL+ IMVDRHPQFRHSRCLFV+RTDGGWIDF
Sbjct: 121 GERLDFEDEKIVMEKLLPYHPYSKDKIGCGLDFIMVDRHPQFRHSRCLFVVRTDGGWIDF 180

Query: 181 SYQKCLRAYIRNKYPLYADRFIREHFKRGS 197
           SYQKCLRAY+R+KYP +A+RFIREHFKR S
Sbjct: 181 SYQKCLRAYVRDKYPSHAERFIREHFKRAS 206

[Close]

BLAST of CmaCh01G018140 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 131.0 bits (328), Expect = 1.1e-30
Identity = 54/114 (47.37%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 81  EEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESI 140
           E++IL    P++   + ILHS +Y + +RL+ E E+ +++ LL +HP  E KIGCG++ I
Sbjct: 101 EDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYI 160

Query: 141 MVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKR 195
           MV  HP F  SRC+F++R DG  +DFSY KC++  I+ KYPLYAD FI  HF++
Sbjct: 161 MVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of CmaCh01G018140 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 129.8 bits (325), Expect = 2.4e-30
Identity = 54/114 (47.37%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 81  EEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESI 140
           E++IL    P++   + ILHS +Y + +RL+ E E+ +++ LL +HP  E KIGCG++ I
Sbjct: 101 EDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYI 160

Query: 141 MVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKR 195
           MV  HP F  SRC+F++R DG  +DFSY KC++  I+ KYPLYAD FI  HF++
Sbjct: 161 MVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of CmaCh01G018140 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 112.5 bits (280), Expect = 4.0e-25
Identity = 47/116 (40.52%), Postives = 79/116 (68.10%), Query Frame = 0


Query: 79   NQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLE 138
            ++E+E+LSD++P++   ++I+H + Y DG+ ++ +D+  V++++L  HP  E K+G G++
Sbjct: 1734 SEEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVD 1793

Query: 139  SIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKR 195
             I VD+H  F  SRC FV+ TDG   DFSY+K L  Y+  KYP  A+ FI ++F +
Sbjct: 1794 FITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of CmaCh01G018140 vs. TAIR 10
Match: AT1G63020.1 (nuclear RNA polymerase D1A )

HSP 1 Score: 53.5 bits (127), Expect = 2.2e-07
Identity = 39/120 (32.50%), Postives = 59/120 (49.17%), Query Frame = 0


Query: 77   WKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCG 136
            WKN        I+ +    K ILHS  Y   E L   DE + V  +L  HP++ +KIG G
Sbjct: 1336 WKN--------IELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPG 1395

Query: 137  LESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYADRFIREHFKRGS 196
            ++ I V +  +   S C  V+R DG + DFSY KC+    +   P   + +  ++ K G+
Sbjct: 1396 VKGIRVAK-SKHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1443

The following BLAST results are available for this feature:

BLAST of CmaCh01G018140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C642	1.5e-29	47.37	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	1.7e-28	37.43	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q5D869	5.6e-24	40.52	DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1...	[more]
Q9LQ02	3.1e-06	32.50	DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]

BLAST of CmaCh01G018140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J4G4	3.7e-111	100.00	protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1FNQ4	3.8e-108	96.94	protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1C4J4	6.7e-97	87.24	protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A1S3BU88	6.5e-92	81.12	protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1	[more]
A0A6J1IHJ1	3.6e-90	81.12	protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477...	[more]

BLAST of CmaCh01G018140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982288.1	7.6e-111	100.00	protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]
KAG6608449.1	2.4e-109	97.96	Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7037785.1	4.6e-108	97.94	Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022940238.1	7.8e-108	96.94	protein DCL homolog, chloroplastic-like [Cucurbita moschata]	[more]
XP_023523235.1	1.0e-107	95.41	protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh01G018140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46630.1	5.9e-61	57.14	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	1.1e-30	47.37	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	2.4e-30	47.37	Protein of unknown function (DUF3223)	[more]
AT2G40030.1	4.0e-25	40.52	nuclear RNA polymerase D1B	[more]
AT1G63020.1	2.2e-07	32.50	nuclear RNA polymerase D1A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.40		coord: 82..178 e-value: 2.2E-24 score: 87.7
None	No IPR available	PFAM	PF11523	DUF3223	coord: 96..172 e-value: 4.9E-28 score: 97.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..63
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..71
None	No IPR available	PANTHER	PTHR33415:SF4	DCL PROTEIN (DUF3223)	coord: 1..195
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 1..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G018140.1	CmaCh01G018140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:1901259	chloroplast rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003729	mRNA binding