CmaCh01G018120 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G018120
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Isoaspartyl peptidase/L-asparaginase
Location	Cma_Chr01: 12043552 .. 12045147 (-)
RNA-Seq Expression	CmaCh01G018120
Synteny	CmaCh01G018120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCATTAGAAAACAGAGTGAAGAAGAAGGCAGAGACAGAGCGATGGGGGGTTGGGCCATAGCGGTTCACGGCGGGGCTGGCGTGGATCCTAATCTCCCACTGCAACGTCAAGAAGATGCAAAAATGCTTCTGACTCGCTGCCTTGATCTTGGCATTCACGCTCTTCGGTCAAATTGTTCTGCTATTGATGTCGTTGAACTCGTCGTACGCTCTCTCCGATCCCTTTTCGGTTTCTTTTCTTTCTCTAATAGATTATTTCCATGCGTCATTTCATTCTCTGCGACATATTCTTGAATTATATTGCGATTTTGAAGGTTAGAGAACTTGAAACCGATCCCCTGTTCAACTCAGGGCGTGGATCTGCCCTGACAGAGGAGGGAACCGTTGAAATGGAAGCCAGCATCATGGACGGACCAAAACGGCGATGCGGGGCGGTGTCTGGACTTACCACTGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCCCCTCATTCCTATCTGGCTTTTTCTGGCGCTGAAAAATTCGCTAGAGAACAGGTTTGATTTCCATCTTTATTCTCCCTTTTTTCCGGTTCTAAAGCGGAATATGATTTCCATCTTTATCTGGCTTTTTCGGACGGAATTGAATCTGTGCTAGGGTGTTGAACTTGAGTGCAATGACTATTTCGTCACCGAAGAAAACGTCGGAATGCTTGAACTAGCCAGAGAAGCCAACAGTATATGGGTACCCCTCCGATTCAAGTATTTTTAACTAAATATGATTCAAAATAACCGTTCGAAACGGACTTGACATTTTCTGGTTCGATTTGTAACAGTTAGATTACAGGGTCCCAAACGAGAAGTGTAGCGCGAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATTAGCGTGTACGCGCCAGAGACTGTGGGCTGCGTGGTGGTCGACAGTGAAGGAAGGTGCGCCGCCGCCACGTCCACCGGCGGCCTGATGAACAAAAAGATCGGGCGCATCGGAGACTCTCCGCTGATCGGTTCAGGGACGTACGCGTGCGGGGTGTGTGGGGTATCGTGCACTGGAGAAGGGGAGGCGATCATAAGAGGGACATTGGCAAGAGAAGTAGCGGCGGTGATGGAGTACAAAGGTCTGGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGGCTGGACGAAGGAAAGGCCGGATTGATTGCCGTGTCGAGCAATGGTGAAGTGGTTTGTGGGTTCAACACCACCGGAATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGGATTTGGAAATAAATTGGGATATGATTGGTTAGATAATTTAAGATTGGGATGTGAAGTTTTGTGGGTTTTAATGCTTTGTAGTGTGCTTTGTTTTTTCAGTAACTGTGTGAAATCTTCTTCAGAATTTTCTGGATTTAGATTCAGAGGCAGATCCATCTTGGCAAATAAAAAATAATGCCCTTAGCCCTGACTACTACGATCTTTTATTTTCTAATCAAATTCTTGATAATCTTAAATTATTAAAGAAGGAAAATAGTTATGAAACAGCAGTATGATTCATGAACACAGGGAAG

mRNA sequence

GGCATTAGAAAACAGAGTGAAGAAGAAGGCAGAGACAGAGCGATGGGGGGTTGGGCCATAGCGGTTCACGGCGGGGCTGGCGTGGATCCTAATCTCCCACTGCAACGTCAAGAAGATGCAAAAATGCTTCTGACTCGCTGCCTTGATCTTGGCATTCACGCTCTTCGGTCAAATTGTTCTGCTATTGATGTCGTTGAACTCGTCGTTAGAGAACTTGAAACCGATCCCCTGTTCAACTCAGGGCGTGGATCTGCCCTGACAGAGGAGGGAACCGTTGAAATGGAAGCCAGCATCATGGACGGACCAAAACGGCGATGCGGGGCGGTGTCTGGACTTACCACTGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCCCCTCATTCCTATCTGGCTTTTTCTGGCGCTGAAAAATTCGCTAGAGAACAGGGTGTTGAACTTGAGTGCAATGACTATTTCGTCACCGAAGAAAACGTCGGAATGCTTGAACTAGCCAGAGAAGCCAACAGTATATGGTTAGATTACAGGGTCCCAAACGAGAAGTGTAGCGCGAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATTAGCGTGTACGCGCCAGAGACTGTGGGCTGCGTGGTGGTCGACAGTGAAGGAAGGTGCGCCGCCGCCACGTCCACCGGCGGCCTGATGAACAAAAAGATCGGGCGCATCGGAGACTCTCCGCTGATCGGTTCAGGGACGTACGCGTGCGGGGTGTGTGGGGTATCGTGCACTGGAGAAGGGGAGGCGATCATAAGAGGGACATTGGCAAGAGAAGTAGCGGCGGTGATGGAGTACAAAGGTCTGGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGGCTGGACGAAGGAAAGGCCGGATTGATTGCCGTGTCGAGCAATGGTGAAGTGGTTTGTGGGTTCAACACCACCGGAATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGGATTTGGAAATAAATTGGGATATGATTGGTTAGATAATTTAAGATTGGGATGTGAAGTTTTGTGGGTTTTAATGCTTTGTAGTGTGCTTTGTTTTTTCAGTAACTGTGTGAAATCTTCTTCAGAATTTTCTGGATTTAGATTCAGAGGCAGATCCATCTTGGCAAATAAAAAATAATGCCCTTAGCCCTGACTACTACGATCTTTTATTTTCTAATCAAATTCTTGATAATCTTAAATTATTAAAGAAGGAAAATAGTTATGAAACAGCAGTATGATTCATGAACACAGGGAAG

Coding sequence (CDS)

ATGGGGGGTTGGGCCATAGCGGTTCACGGCGGGGCTGGCGTGGATCCTAATCTCCCACTGCAACGTCAAGAAGATGCAAAAATGCTTCTGACTCGCTGCCTTGATCTTGGCATTCACGCTCTTCGGTCAAATTGTTCTGCTATTGATGTCGTTGAACTCGTCGTTAGAGAACTTGAAACCGATCCCCTGTTCAACTCAGGGCGTGGATCTGCCCTGACAGAGGAGGGAACCGTTGAAATGGAAGCCAGCATCATGGACGGACCAAAACGGCGATGCGGGGCGGTGTCTGGACTTACCACTGTCAAAAACCCGATCTCCCTCGCCCGTCTTGTCATGGACAAATCCCCTCATTCCTATCTGGCTTTTTCTGGCGCTGAAAAATTCGCTAGAGAACAGGGTGTTGAACTTGAGTGCAATGACTATTTCGTCACCGAAGAAAACGTCGGAATGCTTGAACTAGCCAGAGAAGCCAACAGTATATGGTTAGATTACAGGGTCCCAAACGAGAAGTGTAGCGCGAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATTAGCGTGTACGCGCCAGAGACTGTGGGCTGCGTGGTGGTCGACAGTGAAGGAAGGTGCGCCGCCGCCACGTCCACCGGCGGCCTGATGAACAAAAAGATCGGGCGCATCGGAGACTCTCCGCTGATCGGTTCAGGGACGTACGCGTGCGGGGTGTGTGGGGTATCGTGCACTGGAGAAGGGGAGGCGATCATAAGAGGGACATTGGCAAGAGAAGTAGCGGCGGTGATGGAGTACAAAGGTCTGGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGGCTGGACGAAGGAAAGGCCGGATTGATTGCCGTGTCGAGCAATGGTGAAGTGGTTTGTGGGTTCAACACCACCGGAATGTTCAGGGGATGTGCGACGGAGGATGGGTTTATGGAGGTTGGGATTTGGAAATAA

Protein sequence

MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELETDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCGFNTTGMFRGCATEDGFMEVGIWK

Homology

[Close]

BLAST of CmaCh01G018120 vs. ExPASy Swiss-Prot
Match: Q8GXG1 (Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g16150 PE=2 SV=2)

HSP 1 Score: 537.0 bits (1382), Expect = 1.5e-151
Identity = 264/325 (81.23%), Postives = 295/325 (90.77%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP +RQE+AK LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKC--SASVETP 180
           AFSGAE FAR+QGVE+  N+YFVT++NVGML+LA+EANSI  DYR+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGLMNK +GRIGDSPLIG+GTYA   C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVV 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEVV
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIWK 324
           CGFN+ GMFRGCATEDGFMEV IW+
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIWE 325

[Close]

BLAST of CmaCh01G018120 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 365.5 bits (937), Expect = 6.0e-100
Identity = 190/323 (58.82%), Postives = 232/323 (71.83%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPL 180
           LAF  AE FAR  GVE   + +F+T EN+  L+ A+E N + LDY VP+ K       P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPK------VPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of CmaCh01G018120 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.5e-98
Identity = 191/327 (58.41%), Postives = 235/327 (71.87%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R++  +  L  CL +G+ AL+S    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN----EKCSASV 180
           LAF GA+ FA++QGVE   + +F+T ENV  L+LA EAN + +DY   N     +  A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GEAII  T+AR+VAA+ME+KGL L+EA +YV+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVVCGFNTTGMFRGCATEDGFMEVGIW 323
           E+   FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRASATEDGYSEIAIW 322

[Close]

BLAST of CmaCh01G018120 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.5e-98
Identity = 191/327 (58.41%), Postives = 235/327 (71.87%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R++  +  L  CL +G+ AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN----EKCSASV 180
           LAF GA+ FA++QGVE   + + +T ENV  L+LA EAN + +DY   N     K  A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLTNGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GE IIR T+AR+VAA+ME+KGL L+EA ++VI ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 300

Query: 301 EVVCGFNTTGMFRGCATEDGFMEVGIW 323
           E+   FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of CmaCh01G018120 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.2e-97
Identity = 189/327 (57.80%), Postives = 233/327 (71.25%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP +R++  +  L  CL +GI AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL TV NPISLAR VMDK+PH +
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIF 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN----EKCSASV 180
           LAF GA+   ++QGVE   + +F+TEENV  L+LA EAN + +DY   N     +  A  
Sbjct: 121 LAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
            +C VS TG+GEAIIR T+AR+VAA+ME+KGL L+EA + V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVVCGFNTTGMFRGCATEDGFMEVGIW 323
           E+   FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of CmaCh01G018120 vs. ExPASy TrEMBL
Match: A0A6J1IYV7 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481149 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 9.1e-184
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of CmaCh01G018120 vs. ExPASy TrEMBL
Match: A0A6J1FND2 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446743 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 5.9e-183
Identity = 321/323 (99.38%), Postives = 322/323 (99.69%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of CmaCh01G018120 vs. ExPASy TrEMBL
Match: A0A6J1CHT1 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 GN=LOC111011731 PE=4 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.6e-164
Identity = 290/325 (89.23%), Postives = 309/325 (95.08%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSASVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240
           PLQMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 VCGFNTTGMFRGCATEDGFMEVGIW 323
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

[Close]

BLAST of CmaCh01G018120 vs. ExPASy TrEMBL
Match: A0A5D3D9K2 (Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003020 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.2e-162
Identity = 290/322 (90.06%), Postives = 300/322 (93.17%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK  LTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TE NV +L+LA+EAN IWLD RVP E C ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGL+NKK+GRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of CmaCh01G018120 vs. ExPASy TrEMBL
Match: A0A1S3BV13 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC103493576 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.2e-162
Identity = 290/322 (90.06%), Postives = 300/322 (93.17%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK  LTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TE NV +L+LA+EAN IWLD RVP E C ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGL+NKK+GRIGDSPLIG GTYAC VCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of CmaCh01G018120 vs. NCBI nr
Match: XP_022982271.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima])

HSP 1 Score: 652.5 bits (1682), Expect = 1.9e-183
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of CmaCh01G018120 vs. NCBI nr
Match: XP_022941449.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037783.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649.8 bits (1675), Expect = 1.2e-182
Identity = 321/323 (99.38%), Postives = 322/323 (99.69%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIWK 324
           FNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIWK 323

[Close]

BLAST of CmaCh01G018120 vs. NCBI nr
Match: XP_023525179.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.9 bits (1657), Expect = 1.5e-180
Identity = 321/324 (99.07%), Postives = 322/324 (99.38%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNSSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVP-NEKCSASVETPL 180
           AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVP NEKCSASVETPL
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPTNEKCSASVETPL 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240
           QMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG
Sbjct: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300
           VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC
Sbjct: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIWK 324
           GFNTTGMFRGCATEDGFMEVGIWK
Sbjct: 301 GFNTTGMFRGCATEDGFMEVGIWK 324

[Close]

BLAST of CmaCh01G018120 vs. NCBI nr
Match: XP_038896168.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 300/322 (93.17%), Postives = 311/322 (96.58%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ DAK LLTRCLDLGIHALRSN SAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQHDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TE+NVG+LELA+EANSIWLD+RVP+E CSASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVGNDYFITEDNVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240
           MNGL ISVYAPETVGCVVVD+EGRCAAATSTGGL+NKK+GRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLRISVYAPETVGCVVVDTEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of CmaCh01G018120 vs. NCBI nr
Match: XP_022141340.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Momordica charantia])

HSP 1 Score: 587.4 bits (1513), Expect = 7.4e-164
Identity = 290/325 (89.23%), Postives = 309/325 (95.08%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSASVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240
           PLQMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 VCGFNTTGMFRGCATEDGFMEVGIW 323
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

[Close]

BLAST of CmaCh01G018120 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 537.0 bits (1382), Expect = 1.1e-152
Identity = 264/325 (81.23%), Postives = 295/325 (90.77%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP +RQE+AK LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKC--SASVETP 180
           AFSGAE FAR+QGVE+  N+YFVT++NVGML+LA+EANSI  DYR+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGLMNK +GRIGDSPLIG+GTYA   C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVV 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEVV
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIWK 324
           CGFN+ GMFRGCATEDGFMEV IW+
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIWE 325

[Close]

BLAST of CmaCh01G018120 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 4.3e-101
Identity = 190/323 (58.82%), Postives = 232/323 (71.83%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPL 180
           LAF  AE FAR  GVE   + +F+T EN+  L+ A+E N + LDY VP+ K       P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPK------VPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of CmaCh01G018120 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.4e-75
Identity = 143/243 (58.85%), Postives = 175/243 (72.02%), Query Frame = 0


Query: 80  MEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEKFAREQGVELECN 139
           MEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH YLAF  AE FAR  GVE   +
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 DYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQMNGLPISVYAPETVGCVVV 199
            +F+T EN+  L+ A+E N + LDY VP+ K       P       I      TVGCV V
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSPK------VPDNCGDSQIG-----TVGCVAV 120

Query: 200 DSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGVSCTGEGEAIIRGTLAREVA 259
           DS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C +S TG+GE IIRGT+AR+VA
Sbjct: 121 DSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVA 180

Query: 260 AVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCGFNTTGMFRGCATEDGFMEV 319
           A+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV   FNTTGMFR CA+EDG+ E+
Sbjct: 181 ALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEI 232

Query: 320 GIW 323
            IW
Sbjct: 241 AIW 232

[Close]

BLAST of CmaCh01G018120 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.4e-22
Identity = 92/336 (27.38%), Postives = 142/336 (42.26%), Query Frame = 0


Query: 6   IAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELETDPLFN 65
           +AVH GAG   +     +    ++   CL       + +   ID V   ++ LE DP  N
Sbjct: 15  VAVHVGAGY--HAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPSTN 74

Query: 66  SGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARL----------VMDKS 125
           +GRGS LTE+G VE +AS+MDG     G V  +  V+N I +A L          ++ + 
Sbjct: 75  AGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGRI 134

Query: 126 PHSYLAFSGAEKFAREQ-----GVELECNDYFVTEENVGMLELARE------ANSIWLDY 185
           P   L   GA ++ + +     G   E + + VTE         +       A SI    
Sbjct: 135 PPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAE 194

Query: 186 RVP-----NEKCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKI 245
             P     NE C  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K  
Sbjct: 195 EHPRGTENNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKIS 254

Query: 246 GRIGDSPLIGSGTYACG--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQE 299
           GR+G +   GSG +A          + G   +G GE ++RG  ARE    +     G   
Sbjct: 255 GRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPAS 314

[Close]

BLAST of CmaCh01G018120 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 2.4e-19
Identity = 90/328 (27.44%), Postives = 141/328 (42.99%), Query Frame = 0


Query: 6   IAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELETDPLFN 65
           +AVH GAG   +     +    ++   CL       + +   ID V   ++ LE DP  N
Sbjct: 15  VAVHVGAGY--HAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPSTN 74

Query: 66  SGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPIS--LARLVMDKSPHSYLAFS 125
           +GRGS LTE+G VE +AS+MDG     G   G+  V + +S  L++L++       L   
Sbjct: 75  AGRGSNLTEDGHVECDASLMDGDS---GMFGGVGAVPDQVSEMLSKLLL----CWLLVGE 134

Query: 126 GAEKFAREQ-----GVELECNDYFVTEENVGMLELARE------ANSIWLDYRVP----- 185
           GA ++ + +     G   E + + VTE         +       A SI      P     
Sbjct: 135 GARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTEN 194

Query: 186 NEKCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPL 245
           NE C  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K  GR+G +  
Sbjct: 195 NETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAAT 254

Query: 246 IGSGTYACG--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEE 299
            GSG +A          + G   +G GE ++RG  ARE    +     G   A   V+  
Sbjct: 255 YGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLRS 314

The following BLAST results are available for this feature:

BLAST of CmaCh01G018120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GXG1	1.5e-151	81.23	Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 ...	[more]
P50287	6.0e-100	58.82	Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08...	[more]
P30364	2.5e-98	58.41	Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1	[more]
Q9ZSD6	2.5e-98	58.41	Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1	[more]
P50288	6.2e-97	57.80	Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1	[more]

BLAST of CmaCh01G018120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IYV7	9.1e-184	100.00	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FND2	5.9e-183	99.38	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1CHT1	3.6e-164	89.23	probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 G...	[more]
A0A5D3D9K2	1.2e-162	90.06	Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BV13	1.2e-162	90.06	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of CmaCh01G018120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982271.1	1.9e-183	100.00	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima]	[more]
XP_022941449.1	1.2e-182	99.38	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447...	[more]
XP_023525179.1	1.5e-180	99.07	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo]	[more]
XP_038896168.1	1.2e-169	93.17	probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida]	[more]
XP_022141340.1	7.4e-164	89.23	probable isoaspartyl peptidase/L-asparaginase 2 [Momordica charantia]	[more]

BLAST of CmaCh01G018120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16150.1	1.1e-152	81.23	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.1	4.3e-101	58.82	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.2	2.4e-75	58.85	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.1	1.4e-22	27.38	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.2	2.4e-19	27.44	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000246	Peptidase T2, asparaginase 2	PFAM	PF01112	Asparaginase_2	coord: 4..322 e-value: 8.3E-101 score: 337.1
IPR000246	Peptidase T2, asparaginase 2	PANTHER	PTHR10188	L-ASPARAGINASE	coord: 1..322
None	No IPR available	GENE3D	3.60.20.30	(Glycosyl)asparaginase	coord: 193..323 e-value: 3.0E-43 score: 149.1
None	No IPR available	PIRSR	PIRSR600246-2	PIRSR600246-2	coord: 3..319 e-value: 2.5E-90 score: 300.7
None	No IPR available	PIRSR	PIRSR600246-1	PIRSR600246-1	coord: 3..319 e-value: 2.5E-90 score: 300.7
None	No IPR available	PIRSR	PIRSR600246-3	PIRSR600246-3	coord: 3..319 e-value: 2.5E-90 score: 300.7
None	No IPR available	PANTHER	PTHR10188:SF36	PEPTIDASE T2, ASPARAGINASE 2, NUCLEOPHILE AMINOHYDROLASE-RELATED	coord: 1..322
None	No IPR available	CDD	cd04701	Asparaginase_2	coord: 5..315 e-value: 2.17952E-122 score: 350.223
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 4..320

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G018120.1	CmaCh01G018120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016540	protein autoprocessing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004067	asparaginase activity
molecular_function	GO:0008798	beta-aspartyl-peptidase activity
molecular_function	GO:0016787	hydrolase activity