CmaCh01G017330 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G017330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme CWINV1-like
LocationCma_Chr01: 11727993 .. 11731266 (-)
RNA-Seq ExpressionCmaCh01G017330
SyntenyCmaCh01G017330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCCACATCGGTCACCAATTGCCATGATCATGACCGTCGACGTTGTTGTGACCTTAAAGTTTATCAAAAAACTGCAAGTTGAAGATTAATGATCAGGTGAAGGAGACGAAGTCAACGAGAAGTCGCATCGACTCTCCATTAATTCCGGCGATCCCCCGCCGGCGATTTCAGCAGCTCTCACTCGGCTACGCCATTAAAATCAGAGAATGAGCAAACCCTTAACTCGGGCCGTTATCTTTTGCATCTGGTTGGCTTTTAATGGAGTCGATGCAGCCTCAAATCAGCCCTACAGAACAGGTTTTCACTTCCAGCCTCCTAAAAATTGGATGAACGGTAAAAATCTCGCAAAACTTTGTCGTTCATTTGGCGATTTGTCTTTTTTCTCTATTTGGAATTGACAATCTACCCTATTGAGTATTGATTAAATTGCTTTGTGTCATTTGCTGGGCTGTTTTTTGGCTGATTACGACCACAAAGATCCCAATGGTACAGTTTCCCCCAAATTTTGTCAATAATTTCCCTCTTTTAGCTGTAGTAGCGATGTAATGAGTTCATAATTGCAGGACCAATGTATTACAAGGGAGTTTATCATCTGTTCTATCAGTATAATCCTTATAGTGCAATTTGGGGAAACATTACATGGGCCCATTCAATCTCGTATGATCTTGTAGATTGGATTCATTTAGAACATGCTATTTCTCCAACTGAACCCTATGAAGCCAATGGTTGTTGGTCTGGTTCTGCAACAGTTCTTGTTGATGGAAAACCTTCCATCTTATACACTGGAGCCAACTCTAAGAATCAGCAATTTCAAAATCTAGCTGTGCCCAAGAATCCATCAGATCCTTTACTGAAGGAATGGATTAAATCACCTCATAATCCTCTGATTTCCCCTGTTGATGACATTGATCCTTTGAACTTTAGGGACCCTACCACTGCTTGGCTTGGCCCAGACAGGTTATGGAGGATCATTGTTGGAGGGGAGATTGGTGGTCAAGGAGTGGCGATTTTGTATCGTAGTGAAGATTTTGTAAATTGGACTCGGGGTAAGACTCCGCTTCATACGTCGAACAAAACTGGGATGTGGGAGTGCCCGGATTTTTATCCAGTGAGTATCAATGGCAGTAATGGACTTGACACCTCTGTTCTAGCTGGGTTAATCAAACATGTGCTCAAGGCGAGCTTTAATAATAATGATCACTATGTACTGGGGAGATACACTCCTGAAATGGATACATTTTCGGTTGAGACTAACTTCTTGAGCAATGGCTCGGATATGCGATACGATTATGGGAAATTCTATGCTTCGAAATCGTTTTATGATAGTGATAAGAAGAGAAGAATACTATGGGGATGGATATTGGAATCGGACAGTGAAGCTGATGATATTAGCAAAGGATGGTCTGGCCTTCAGGTATTTGAATCAACTGAGCTTCATAGTTCTAAACTTCTGATATGAGTACCCTGTTTATATTAACAGCTTATTCTATATTGCTTTTCAATTACATTTCATAGTCAATTCCTAGAAGTATATTACTTGATGAATCTGGAAAACAATTATTACTATGGCCAATTAAAGAAATTGAGAAACTACGTACAAAGCAAGTGAGTTTTGATGATATGGATCTCAAAAGTGGTTCAGTATTTGAAGTCCCGGGCATTATGGCCGCACAGGTAACTATTCTGGCACTCATTATTTCAGAGTTACAAACAAACTCTCAAAGAAAAGTCGAAAACATTTTCACGTTTGTTTCCTATATGCAAAGGCCGATATCGAGGTTTCGTTCCGCTTATCGGGTCTTAAAGAAGCAGAAGAGATGAATACGAGTTGGGTTGATCCAGAGTTGCTCTGTAAGGAAAAGAAAGCATCAATGAATGGCACGATTGGACCATTTGGGTTGCTGGTTTTAGCTTCTAAGGATTTGGAAGAACAAACTGCAGTCTACTTCCGAGTATTCCGAAAGCAATACGAGAAATATGTTGTGCTCATGTGCAGCGATCAAAGCAGGTTTGTTGGTTCTGTTTTTTTTTTTTTTTTTCTATACTGCTTGATCTGTTATGACTGAAGCAGCAAGCTCACAATTTCAACAGATCTTCTTTGAAGGAGGGGCTTGAGAAAACAACTTATGGAGCATTCGTTGACATAGATCCTGTTCATGAAACGATATCTTTAAGAAGCCTGGTATGTCGCTTAAAGAAACAAAAACATATATGTTCAGTTGGTTATTCTCTTCACTTTCCTTTGTTTTTTGCATTCCAAGATCTTTGTAGCTGCATACTATTGCAAAAGCTTGTATAAATGATCTAATCTATGAATTATATCACCATCTTTGTTATCCTTTTAATCTATATTTGAAAGAAAAACAAAATAGGAATGAACATACCCTTTAGATTAGTACTATTGCATGTATCTTCATGGGTTTGCTTATTCTTGTTCAACGAATTAACTTTGGATCAAAAAACGGCTAAGCTTGCCCAAACCCGTTCTCTAATAAAAATATAAGTCTTTGAGGTCGGTGGTGTTGGGTTCTGGAGACTGCTGCTGCTTATTTATAGCTCATTCAATAGTTATACGTAGTAAAACTATAAATAACATATAAAATAAATGGTGTATACATCTATGTATATTAAAAGGTAAAATTATATATAGTAGATAGTTACATGTGGTAGATAAGATTATATTTGGTAAAACTATATATGGTAAACTGTACCATAAGTTTTTTGAATATGTACTTTTTGCATTCTCTGTATTAACTCATACCGTACGTATCTGAAGTCATCCATATAAACTTTTAGCCAATATTTCTTCTTGCATTGTCTCTCTCTTGTTCTTTGTGTTCATCTAAATAATGTGTTCTCTTTGCATTTTCTCGTTCTTAAATCTCCCCCCTCTTTGTTGTAATAAAAACACCGAGTTTAGAACTTCTTAATATAGTTACCCATTCACTTATTACCATTAGTTCACGTATTTAAGCCTGCACGTGAGGGAGTGCTAAGAATGTCACCTCGTTGAACTTTCCGATATAAAACCCACAACATTCGGGTGTTTTGTTTTGCAGATAGACCATTCAATAGTGGAAAGTTTTGGTGGAAATGGGAAAGCCTGCATCACTGCAAGAGTGTATCCAAAGCTGGCCATCAACCAAGACGCCCATCTTTATGCATTCAACAACGGAACAATGGATGTAAGAATCTCAAAGCTAAGTGGTTGGAGCATGAAGAGAGCACAGATCGTTCCCATCCAAAAGAGAAGAAAACCTTCCATCACTTGA

mRNA sequence

TTTTCCACATCGGTCACCAATTGCCATGATCATGACCGTCGACGTTGTTGTGACCTTAAAGTTTATCAAAAAACTGCAAGTTGAAGATTAATGATCAGGTGAAGGAGACGAAGTCAACGAGAAGTCGCATCGACTCTCCATTAATTCCGGCGATCCCCCGCCGGCGATTTCAGCAGCTCTCACTCGGCTACGCCATTAAAATCAGAGAATGAGCAAACCCTTAACTCGGGCCGTTATCTTTTGCATCTGGTTGGCTTTTAATGGAGTCGATGCAGCCTCAAATCAGCCCTACAGAACAGGTTTTCACTTCCAGCCTCCTAAAAATTGGATGAACGATCCCAATGGACCAATGTATTACAAGGGAGTTTATCATCTGTTCTATCAGTATAATCCTTATAGTGCAATTTGGGGAAACATTACATGGGCCCATTCAATCTCGTATGATCTTGTAGATTGGATTCATTTAGAACATGCTATTTCTCCAACTGAACCCTATGAAGCCAATGGTTGTTGGTCTGGTTCTGCAACAGTTCTTGTTGATGGAAAACCTTCCATCTTATACACTGGAGCCAACTCTAAGAATCAGCAATTTCAAAATCTAGCTGTGCCCAAGAATCCATCAGATCCTTTACTGAAGGAATGGATTAAATCACCTCATAATCCTCTGATTTCCCCTGTTGATGACATTGATCCTTTGAACTTTAGGGACCCTACCACTGCTTGGCTTGGCCCAGACAGGTTATGGAGGATCATTGTTGGAGGGGAGATTGGTGGTCAAGGAGTGGCGATTTTGTATCGTAGTGAAGATTTTGTAAATTGGACTCGGGGTAAGACTCCGCTTCATACGTCGAACAAAACTGGGATGTGGGAGTGCCCGGATTTTTATCCAGTGAGTATCAATGGCAGTAATGGACTTGACACCTCTGTTCTAGCTGGGTTAATCAAACATGTGCTCAAGGCGAGCTTTAATAATAATGATCACTATGTACTGGGGAGATACACTCCTGAAATGGATACATTTTCGGTTGAGACTAACTTCTTGAGCAATGGCTCGGATATGCGATACGATTATGGGAAATTCTATGCTTCGAAATCGTTTTATGATAGTGATAAGAAGAGAAGAATACTATGGGGATGGATATTGGAATCGGACAGTGAAGCTGATGATATTAGCAAAGGATGGTCTGGCCTTCAGTCAATTCCTAGAAGTATATTACTTGATGAATCTGGAAAACAATTATTACTATGGCCAATTAAAGAAATTGAGAAACTACGTACAAAGCAAGTGAGTTTTGATGATATGGATCTCAAAAGTGGTTCAGTATTTGAAGTCCCGGGCATTATGGCCGCACAGGCCGATATCGAGGTTTCGTTCCGCTTATCGGGTCTTAAAGAAGCAGAAGAGATGAATACGAGTTGGGTTGATCCAGAGTTGCTCTGTAAGGAAAAGAAAGCATCAATGAATGGCACGATTGGACCATTTGGGTTGCTGGTTTTAGCTTCTAAGGATTTGGAAGAACAAACTGCAGTCTACTTCCGAGTATTCCGAAAGCAATACGAGAAATATGTTGTGCTCATGTGCAGCGATCAAAGCAGATCTTCTTTGAAGGAGGGGCTTGAGAAAACAACTTATGGAGCATTCGTTGACATAGATCCTGTTCATGAAACGATATCTTTAAGAAGCCTGATAGACCATTCAATAGTGGAAAGTTTTGGTGGAAATGGGAAAGCCTGCATCACTGCAAGAGTGTATCCAAAGCTGGCCATCAACCAAGACGCCCATCTTTATGCATTCAACAACGGAACAATGGATGTAAGAATCTCAAAGCTAAGTGGTTGGAGCATGAAGAGAGCACAGATCGTTCCCATCCAAAAGAGAAGAAAACCTTCCATCACTTGA

Coding sequence (CDS)

ATGAGCAAACCCTTAACTCGGGCCGTTATCTTTTGCATCTGGTTGGCTTTTAATGGAGTCGATGCAGCCTCAAATCAGCCCTACAGAACAGGTTTTCACTTCCAGCCTCCTAAAAATTGGATGAACGATCCCAATGGACCAATGTATTACAAGGGAGTTTATCATCTGTTCTATCAGTATAATCCTTATAGTGCAATTTGGGGAAACATTACATGGGCCCATTCAATCTCGTATGATCTTGTAGATTGGATTCATTTAGAACATGCTATTTCTCCAACTGAACCCTATGAAGCCAATGGTTGTTGGTCTGGTTCTGCAACAGTTCTTGTTGATGGAAAACCTTCCATCTTATACACTGGAGCCAACTCTAAGAATCAGCAATTTCAAAATCTAGCTGTGCCCAAGAATCCATCAGATCCTTTACTGAAGGAATGGATTAAATCACCTCATAATCCTCTGATTTCCCCTGTTGATGACATTGATCCTTTGAACTTTAGGGACCCTACCACTGCTTGGCTTGGCCCAGACAGGTTATGGAGGATCATTGTTGGAGGGGAGATTGGTGGTCAAGGAGTGGCGATTTTGTATCGTAGTGAAGATTTTGTAAATTGGACTCGGGGTAAGACTCCGCTTCATACGTCGAACAAAACTGGGATGTGGGAGTGCCCGGATTTTTATCCAGTGAGTATCAATGGCAGTAATGGACTTGACACCTCTGTTCTAGCTGGGTTAATCAAACATGTGCTCAAGGCGAGCTTTAATAATAATGATCACTATGTACTGGGGAGATACACTCCTGAAATGGATACATTTTCGGTTGAGACTAACTTCTTGAGCAATGGCTCGGATATGCGATACGATTATGGGAAATTCTATGCTTCGAAATCGTTTTATGATAGTGATAAGAAGAGAAGAATACTATGGGGATGGATATTGGAATCGGACAGTGAAGCTGATGATATTAGCAAAGGATGGTCTGGCCTTCAGTCAATTCCTAGAAGTATATTACTTGATGAATCTGGAAAACAATTATTACTATGGCCAATTAAAGAAATTGAGAAACTACGTACAAAGCAAGTGAGTTTTGATGATATGGATCTCAAAAGTGGTTCAGTATTTGAAGTCCCGGGCATTATGGCCGCACAGGCCGATATCGAGGTTTCGTTCCGCTTATCGGGTCTTAAAGAAGCAGAAGAGATGAATACGAGTTGGGTTGATCCAGAGTTGCTCTGTAAGGAAAAGAAAGCATCAATGAATGGCACGATTGGACCATTTGGGTTGCTGGTTTTAGCTTCTAAGGATTTGGAAGAACAAACTGCAGTCTACTTCCGAGTATTCCGAAAGCAATACGAGAAATATGTTGTGCTCATGTGCAGCGATCAAAGCAGATCTTCTTTGAAGGAGGGGCTTGAGAAAACAACTTATGGAGCATTCGTTGACATAGATCCTGTTCATGAAACGATATCTTTAAGAAGCCTGATAGACCATTCAATAGTGGAAAGTTTTGGTGGAAATGGGAAAGCCTGCATCACTGCAAGAGTGTATCCAAAGCTGGCCATCAACCAAGACGCCCATCTTTATGCATTCAACAACGGAACAATGGATGTAAGAATCTCAAAGCTAAGTGGTTGGAGCATGAAGAGAGCACAGATCGTTCCCATCCAAAAGAGAAGAAAACCTTCCATCACTTGA

Protein sequence

MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQIVPIQKRRKPSIT
Homology
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BLAST of CmaCh01G017330 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 1.1e-192
Identity = 320/537 (59.59%), Postives = 407/537 (75.79%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLVDWI 84
           NQPYRTGFHFQPPKNWMNDPNGPM YKG+YHLFYQ+NP  A+WGNI WAHS S DL++W 
Sbjct: 48  NQPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWD 107

Query: 85  HLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLKE 144
               AI P+ P++ NGCWSGSAT+L +GKP ILYTG + KNQQ QN+A PKN SDP L+E
Sbjct: 108 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 167

Query: 145 WIKSPHNPLISP--VDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSEDFV 204
           W KSP NPL++P  V+ I+  +FRDPTTAWLG D+ WR+I+G +I  +G+AI Y S+DF+
Sbjct: 168 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 227

Query: 205 NWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLA---GLIKHVLKASFNN--ND 264
            W +   PLH  + +GMWECPDF+PV+  GSNG++TS       ++KHVLK S ++  +D
Sbjct: 228 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHD 287

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +G Y    D F  +  F  +G+  RYDYGK+YASK+F+DS K RRILWGW  ES S 
Sbjct: 288 YYTIGTYDRVKDKFVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSV 347

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV-SFDDMDLKSGSVFEVP 384
            DD+ KGWSG+Q+IPR I LD SGKQL+ WP++E+E+LRTKQV +  +  LKSGS  EV 
Sbjct: 348 EDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVY 407

Query: 385 GIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLE 444
           G+ AAQAD+EV F++  L++A+ +  SW DP+L+C +   S+   +GPFGL+VLASK+LE
Sbjct: 408 GVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLE 467

Query: 445 EQTAVYFRVF--RKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLI 504
           E T+VYFR+F  R+   KYVVLMCSDQSRSSLKE  +KTTYGAFVDI+P H+ +SLR+LI
Sbjct: 468 EYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALI 527

Query: 505 DHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQI 552
           DHS+VESFGG G+ACIT+RVYPKLAI + +HL+AFN G   V +  L+ WSM  AQI
Sbjct: 528 DHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 583

[Close]
BLAST of CmaCh01G017330 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 656.4 bits (1692), Expect = 3.0e-187
Identity = 318/539 (59.00%), Postives = 394/539 (73.10%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWG-NITWAHSISYDLVDW 84
           NQPYRTG+HFQP KNWMNDPNGPM YKG+YHLFYQYNPY A+W   I W HS S DLV+W
Sbjct: 35  NQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNW 94

Query: 85  IHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLK 144
           I    A +P++P + NGCWSGS T+L +GKP ILYTG +    Q QN+AVP N SDP L+
Sbjct: 95  ISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLR 154

Query: 145 EWIKSPHNPLI--SPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGG-QGVAILYRSED 204
           EW K P NPL+  + V+ I+P  FRDPTTAWLG D  WR+IVG      +G+AILY+S D
Sbjct: 155 EWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRD 214

Query: 205 FVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDT-SVLAGLIKHVLKASF--NNND 264
           F NWT+   PLH  + TGMWECPDF+PVSI GS+G++T SV    IKHVLK S     +D
Sbjct: 215 FFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHD 274

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +G Y  E D +  +  F+ N S  R DYGK+YASK+FYD  KKRRILWGW+ ES   
Sbjct: 275 YYTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPA 334

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPG 384
            DDI KGWSGLQS PR I LDESGK+LL WPI+EIE LR +QV++    LK+GS  +V G
Sbjct: 335 KDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHG 394

Query: 385 IMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLEE 444
           + AAQAD+EVSF++  L++A+ +  SW DP+ +C +   S+   +GPFGL+VLAS D+EE
Sbjct: 395 VTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEE 454

Query: 445 QTAVYFRVFRKQYE-----KYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRS 504
            T+VYFR+F+   +     KYVVLMCSDQSRSSL +  +K+T+GAFV IDP H+TISLR+
Sbjct: 455 YTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRT 514

Query: 505 LIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQI 552
           LIDHSIVES+GG G+ CIT+RVYPKLAI ++A+L+ FN GT  V I  LS WS+K AQI
Sbjct: 515 LIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 573

[Close]
BLAST of CmaCh01G017330 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 7.1e-181
Identity = 296/534 (55.43%), Postives = 384/534 (71.91%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIW-GNITWAHSISYDLVDW 84
           + PYRT +HFQ PKNWMNDPNGPM YKG+YHLFYQY PY  +W   I W HS S DL++W
Sbjct: 52  DDPYRTAYHFQSPKNWMNDPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINW 111

Query: 85  IHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLK 144
                A+SP+EPY+ NGCWSGS T+L   KP ILYTG N+KN Q QNLA+PKN SDP LK
Sbjct: 112 TQQPIALSPSEPYDINGCWSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLK 171

Query: 145 EWIKSPHNPLISPV----DDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSE 204
           EWIK P NPL++      ++I+  +FRDP+TAW   D  WR+IVG + G +G+A+L+ S+
Sbjct: 172 EWIKLPQNPLMAGTPTNNNNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSD 231

Query: 205 DFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLAGLIKHVLKASF--NNND 264
           DFV W     PLH++   G+WECPDF+PV +  S G DTS++   +KHVLK S      +
Sbjct: 232 DFVKWNNTGNPLHSTEGNGIWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYE 291

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +GRY  E D +  +   + +   +RYDYGKFYASKSF+D +  RRILWGW+ ES  +
Sbjct: 292 YYTIGRYDIEKDIYVPDEGSIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQ 351

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPG 384
           ADDI KGWSG+Q+IPR+++LD+SGKQL+ WP+ E++ LR   V      +K GS+ E+  
Sbjct: 352 ADDIKKGWSGVQAIPRTVVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQ 411

Query: 385 IMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLEE 444
           I A+QAD+E+SF++      EE++++  +P++LC +K AS+ G  GPFGLL LAS  LEE
Sbjct: 412 ITASQADVEISFKIPESNYVEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGLEE 471

Query: 445 QTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLIDHS 504
            TAV+FR+F K   KYVVLMCSDQ+RSSL    +K ++G FVD+DP++E +SLR LIDHS
Sbjct: 472 YTAVFFRIF-KGPNKYVVLMCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHS 531

Query: 505 IVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQI 552
           IVESF   GK+CITARVYP +AIN  A LY FNNGT DV+I+KLS WSMK+AQI
Sbjct: 532 IVESFSAKGKSCITARVYPTMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQI 584

[Close]
BLAST of CmaCh01G017330 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 627.9 bits (1618), Expect = 1.1e-178
Identity = 303/534 (56.74%), Postives = 390/534 (73.03%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIW--GNITWAHSISYDLVD 84
           NQPYRTG+HFQPPKNWMNDPNGPM YKG+YHLFYQ+N   A+       W H+ S DL++
Sbjct: 39  NQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLIN 98

Query: 85  WIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLL 144
           WI L  AI P+ P + NGCWSGS T+L +GKP ILYTG +  N+Q QNL  PKN +DP L
Sbjct: 99  WITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYL 158

Query: 145 KEWIKSPHNPLI--SPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSED 204
           + W KSP NPL+  SPV+ I+   FRDPTTAW G D  WRI  G + G +G+AIL+ S+D
Sbjct: 159 RHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKD 218

Query: 205 FVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLAG-LIKHVLKASFNN--ND 264
           FV W +   PLH  + TGMWECPDF+PV+   S GLDTS  +G ++KHVLK S  +  ND
Sbjct: 219 FVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFND 278

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +G Y    D +  +  F+ + +  RYDYGKFYASK+FYDS  +RRILWGW+ ES  E
Sbjct: 279 YYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPE 338

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPG 384
            D+I KGW+GLQ+IPR + LDESGK+L+ WP+KEIE+LRT QV + +  LK GSV EV G
Sbjct: 339 KDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHG 398

Query: 385 IMAAQADIEVSFRLSG--LKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDL 444
           + A QAD+EV F++SG  L++A+ +   W DP+L+C ++ AS    +GPFGL+VLASK++
Sbjct: 399 VTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASSG--LGPFGLMVLASKNM 458

Query: 445 EEQTAVYFRVFR--KQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSL 504
           EE T+V  R+FR  +  +++VV+MCSDQS SSL++G +KTTYGAF+DI P ++ ISLR+L
Sbjct: 459 EEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP-YQPISLRTL 518

Query: 505 IDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMK 548
           ID SIVESFGG GK CIT+RVYPKLAI +  HL+AFN G+ +V +  LS WSMK
Sbjct: 519 IDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMK 569

[Close]
BLAST of CmaCh01G017330 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 5.3e-176
Identity = 289/511 (56.56%), Postives = 376/511 (73.58%), Query Frame = 0 

Query: 23  ASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLVD 82
           +++QPYRT +HFQP KNW+NDPNGPM Y G YHLFYQYNP  A+WGNI WAHS+S DLV+
Sbjct: 41  SNHQPYRTAYHFQPLKNWINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVN 100

Query: 83  WIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLL 142
           W  L+HAI P++P +  GCWSGSAT+L  GKP+ILYTG +  N Q QN+A+PKN SDPLL
Sbjct: 101 WTPLDHAIHPSQPSDIKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLL 160

Query: 143 KEWIKSPHNPLISP--VDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSED 202
           +EW KSP NPL+ P   + I+  +FRDPTT+WLG D  WR+++G +I  +G+AILY+S++
Sbjct: 161 REWKKSPKNPLMEPTIANKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKN 220

Query: 203 FVNWTRGKTPLHTSNKTGMWECPDFYPVSING--SNGLDTSVLA-GLIKHVLKASFNN-- 262
           FV+W   K PLH++  TGMWECPDFYPV        G+DTS      ++HVLK S ++  
Sbjct: 221 FVDWVEAKHPLHSAEGTGMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTK 280

Query: 263 NDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESD 322
           +DHY++G Y    D F  E  F  NG  +RYDYGK+YASK+F+D  K RRIL GW+ ES 
Sbjct: 281 HDHYLIGSYDVVKDVFVPENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESS 340

Query: 323 SEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEV 382
           S ADD+ KGWSG+ +IPR I L ESGKQL+ WP+KEIE LR   V++    +K G    +
Sbjct: 341 SVADDVKKGWSGIHTIPREIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISI 400

Query: 383 PGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDL 442
            G+ + QAD+E+SF +S L + E +   W+DP+LLC +K A + G +GPFGLLV AS+ L
Sbjct: 401 TGVDSVQADVEISFEISDLGKVESLR-KWIDPQLLCSQKGAGVKGGVGPFGLLVFASQGL 460

Query: 443 EEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLID 502
           +E TAV+FR+F+ Q +K +VLMCSDQSRSSL +  + T+YG FVD+DP+HE +SLR+LID
Sbjct: 461 KEYTAVFFRIFKYQ-DKNLVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLID 520

Query: 503 HSIVESFGGNGKACITARVYPKLAINQDAHL 527
           HS+VESFGG G+AC+TARVYP LAI+  A L
Sbjct: 521 HSVVESFGGEGRACVTARVYPTLAIHDKAML 549

[Close]
BLAST of CmaCh01G017330 vs. ExPASy TrEMBL
Match: A0A6J1IZ33 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111481203 PE=3 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR
Sbjct: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
           LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
           DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV
Sbjct: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420
           SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG
Sbjct: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420

Query: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480
           TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD
Sbjct: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480

Query: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540
           IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK
Sbjct: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540

Query: 541 LSGWSMKRAQIVPIQKRRKPSIT 564
           LSGWSMKRAQIVPIQKRRKPSIT
Sbjct: 541 LSGWSMKRAQIVPIQKRRKPSIT 563

[Close]
BLAST of CmaCh01G017330 vs. ExPASy TrEMBL
Match: A0A6J1FLF0 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111446480 PE=3 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 550/563 (97.69%), Postives = 559/563 (99.29%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           M+KPLTRAVIF IWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MNKPLTRAVIFGIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSAT+L+DGKPSILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATILLDGKPSILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR
Sbjct: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           IIVGGEIGGQG+A+LYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 IIVGGEIGGQGLAVLYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
           L GLIKHVLKASFNNNDHYVLGRYTPE DTFSVETNFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 LDGLIKHVLKASFNNNDHYVLGRYTPETDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
           DKKRRILWGWILESDSE DDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV
Sbjct: 301 DKKRRILWGWILESDSEVDDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420
           SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLK+AEEMNTSWVDP+LLCKEKKASMNG
Sbjct: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKKAEEMNTSWVDPQLLCKEKKASMNG 420

Query: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480
           TIGPFGLLVLASK+LEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSL+EGLEKTTYGAFVD
Sbjct: 421 TIGPFGLLVLASKNLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLQEGLEKTTYGAFVD 480

Query: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540
           IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK
Sbjct: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540

Query: 541 LSGWSMKRAQIVPIQKRRKPSIT 564
           LSGWSMKRAQIVPIQKRRKPSIT
Sbjct: 541 LSGWSMKRAQIVPIQKRRKPSIT 563

[Close]
BLAST of CmaCh01G017330 vs. ExPASy TrEMBL
Match: A0A6J1CF86 (beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Momordica charantia OX=3673 GN=LOC111011145 PE=3 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.0e-295
Identity = 482/563 (85.61%), Postives = 517/563 (91.83%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           M KPL   V    WLA+ GV+AAS QPYRT FHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MKKPLIWVVPLFFWLAYYGVEAASYQPYRTAFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHS SYDLVDW+HLEHA+ PTEPYE NGCWSGSAT+++  KP+ILYTG
Sbjct: 61  NPYSAIWGNITWAHSXSYDLVDWVHLEHALYPTEPYEVNGCWSGSATIILGEKPAILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           AN KNQQFQNLAVPKNPSDPLLKEWIKSPHNPLI PVDDIDP NFRDPTTAWLG D LWR
Sbjct: 121 ANFKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLIVPVDDIDPQNFRDPTTAWLGSDGLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           +IVGGEIGG+G+AILYRS+DFV+WTR K+PLHTS +TGMWECPDFYPVSINGSNGLDTS 
Sbjct: 181 VIVGGEIGGRGMAILYRSKDFVDWTRSKSPLHTSTETGMWECPDFYPVSINGSNGLDTSA 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
              L+KHVLKASF++NDHYVLG Y  E DTFSVET+FLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 EGKLVKHVLKASFHDNDHYVLGSYVRETDTFSVETDFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
           DK+RRILWGWI ESDSEADDI KGWSGLQSIPRSILL ESGKQL+ WPIKEIEKLRTKQV
Sbjct: 301 DKERRILWGWIKESDSEADDIGKGWSGLQSIPRSILLXESGKQLVQWPIKEIEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420
           SFDDMDLKSGSVFEVPGI AAQADIE+ FRLSGLKE+EEMN SW+DP+LLCKE KAS+NG
Sbjct: 361 SFDDMDLKSGSVFEVPGITAAQADIEILFRLSGLKESEEMNVSWIDPQLLCKENKASVNG 420

Query: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480
            IGPFGLLVLASK+L EQTAVYFRV RKQY+KY VLMCSDQSRSSL+EGLEKTTYGAFVD
Sbjct: 421 MIGPFGLLVLASKELTEQTAVYFRVLRKQYDKYAVLMCSDQSRSSLREGLEKTTYGAFVD 480

Query: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540
           IDP+HET+SLRSLIDHSI+ESFGGNGKACITARVYP LAINQDAHLYAFNNGT+DVRISK
Sbjct: 481 IDPLHETLSLRSLIDHSIIESFGGNGKACITARVYPNLAINQDAHLYAFNNGTVDVRISK 540

Query: 541 LSGWSMKRAQIVPIQKRRKPSIT 564
           LSGWSMK+AQIVPIQKRRK + T
Sbjct: 541 LSGWSMKKAQIVPIQKRRKKAPT 563

[Close]
BLAST of CmaCh01G017330 vs. ExPASy TrEMBL
Match: A0A1S3BVQ3 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493665 PE=3 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.7e-292
Identity = 477/563 (84.72%), Postives = 515/563 (91.47%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           M KPL   +  C  LA+NGV+  SNQPYRT FHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MRKPLNWVIPLCFLLAYNGVETVSNQPYRTAFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDW+HLEHA+SPTEPYE NGCWSGSAT+L+D +PSILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWVHLEHALSPTEPYETNGCWSGSATILLDEQPSILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANSKNQQFQNLA+PKN SDPLLKEWIKSP NPLI+PVDDIDP NFRDPTTAWLG DRLWR
Sbjct: 121 ANSKNQQFQNLALPKNRSDPLLKEWIKSPQNPLIAPVDDIDPSNFRDPTTAWLGQDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           +IVGGEIGG G+AILYRSEDFVNWTR K+PLH+SN+TGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 VIVGGEIGGSGMAILYRSEDFVNWTRSKSPLHSSNETGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
             GLIKHVLKASFN+ DHYVLG Y P  DT+SVE NFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 HDGLIKHVLKASFNDTDHYVLGSYIPGTDTYSVENNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
            KKRR+LWGWI ESDSE DD +KGWSGLQSIPR+ILL ESG+QL+ WPIKE+EKLRTKQV
Sbjct: 301 GKKRRLLWGWIKESDSEGDDTTKGWSGLQSIPRTILLGESGRQLVQWPIKELEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKE-KKASMN 420
           SF+D+DLKSGSVFEVPGI AAQADIE+SFRLSGLKEAEEMN+SW DP+LLCKE  KA   
Sbjct: 361 SFNDVDLKSGSVFEVPGITAAQADIEISFRLSGLKEAEEMNSSWNDPQLLCKENNKALWK 420

Query: 421 GTIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFV 480
           G IGPFGLLVLASKDL EQTAVYFRVFR QY+KYVVLMCSDQSRSS +EGLEK TYGAFV
Sbjct: 421 GAIGPFGLLVLASKDLTEQTAVYFRVFRNQYDKYVVLMCSDQSRSSQREGLEKATYGAFV 480

Query: 481 DIDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRIS 540
           D+DP+ ETISLRSLIDHSI+ESFGGNGKACITARVYPK+AINQ+AHLYAFNNG+MDVRIS
Sbjct: 481 DMDPLRETISLRSLIDHSIIESFGGNGKACITARVYPKIAINQEAHLYAFNNGSMDVRIS 540

Query: 541 KLSGWSMKRAQIVPIQKRRKPSI 563
           +LSGWSMKRAQ+VPIQKRRK  I
Sbjct: 541 RLSGWSMKRAQVVPIQKRRKSPI 563

[Close]
BLAST of CmaCh01G017330 vs. ExPASy TrEMBL
Match: A0A0A0L043 (Cell wall invertase 1 OS=Cucumis sativus OX=3659 GN=Csa_4G646140 PE=2 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.5e-292
Identity = 475/563 (84.37%), Postives = 514/563 (91.30%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           M KP    V+ C  LA+NGVDA SNQPYRT FHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MKKPFNWMVLLCFLLAYNGVDAVSNQPYRTAFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDW+HLEHA+SPTEPYE NGCWSGSAT+L+D +P+ILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWVHLEHALSPTEPYETNGCWSGSATILLDEQPAILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANS+NQQFQNLA+PKN SDPLLK+WIKSPHNPLI+PVDDIDP NFRDPTTAWLG DRLWR
Sbjct: 121 ANSENQQFQNLALPKNRSDPLLKDWIKSPHNPLIAPVDDIDPSNFRDPTTAWLGQDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           +I+GGEIGG G+AILYRSEDFVNWTR K+PLH SN+TGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 VIIGGEIGGSGMAILYRSEDFVNWTRSKSPLHLSNETGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
             GLIKHVLKASF   DHYVLG Y P  DT+SVE NFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 QDGLIKHVLKASFKEADHYVLGSYIPGTDTYSVENNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
            KKRRILWGWI+ESDSEADD +KGWSGLQSIPR+ILL ESG+QL+ WPIKE+EKLRTKQV
Sbjct: 301 GKKRRILWGWIIESDSEADDTTKGWSGLQSIPRTILLGESGRQLVQWPIKELEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKE-KKASMN 420
           SFDD+DLKSGS+FEVPGI AAQADIE+SFRLSGLKEAEEMN SW DP+ LCKE  KA   
Sbjct: 361 SFDDVDLKSGSLFEVPGITAAQADIEISFRLSGLKEAEEMNASWNDPQFLCKENNKALGK 420

Query: 421 GTIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFV 480
           G IGPFGLLVLASKDL EQTAVYFRVFR QY+KYVVLMCSDQSRSS +EGLEK TYGAFV
Sbjct: 421 GAIGPFGLLVLASKDLTEQTAVYFRVFRNQYDKYVVLMCSDQSRSSQREGLEKATYGAFV 480

Query: 481 DIDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRIS 540
           D+DP+ ETISLRSLIDHSI+ESFGGNGKACITARVYPK+AINQ+AHLYAFNNG+MDVRI+
Sbjct: 481 DMDPLRETISLRSLIDHSIIESFGGNGKACITARVYPKIAINQEAHLYAFNNGSMDVRIT 540

Query: 541 KLSGWSMKRAQIVPIQKRRKPSI 563
           +LSGWSMKRAQ+VPIQKRRK  I
Sbjct: 541 RLSGWSMKRAQVVPIQKRRKSPI 563

[Close]
BLAST of CmaCh01G017330 vs. NCBI nr
Match: XP_022982346.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita maxima])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR
Sbjct: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
           LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
           DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV
Sbjct: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420
           SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG
Sbjct: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420

Query: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480
           TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD
Sbjct: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480

Query: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540
           IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK
Sbjct: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540

Query: 541 LSGWSMKRAQIVPIQKRRKPSIT 564
           LSGWSMKRAQIVPIQKRRKPSIT
Sbjct: 541 LSGWSMKRAQIVPIQKRRKPSIT 563

[Close]
BLAST of CmaCh01G017330 vs. NCBI nr
Match: XP_023524138.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 552/563 (98.05%), Postives = 559/563 (99.29%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           M +PLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MKEPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSAT+L+DGKPSILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATILLDGKPSILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR
Sbjct: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           IIVGGEIGGQG+AILYRSEDFVNWTR KTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 IIVGGEIGGQGLAILYRSEDFVNWTRRKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
           L GLIKHVLKASFNNNDHYVLGRYTPE DTFSVETNFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 LDGLIKHVLKASFNNNDHYVLGRYTPETDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
           DKKRR+LWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV
Sbjct: 301 DKKRRMLWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420
           SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLK+AEEMNTSWVDP+LLCKEKKASMNG
Sbjct: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKKAEEMNTSWVDPQLLCKEKKASMNG 420

Query: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480
           TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD
Sbjct: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480

Query: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540
           IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK
Sbjct: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540

Query: 541 LSGWSMKRAQIVPIQKRRKPSIT 564
           LSGWSMKRAQIVPIQKRRKPSIT
Sbjct: 541 LSGWSMKRAQIVPIQKRRKPSIT 563

[Close]
BLAST of CmaCh01G017330 vs. NCBI nr
Match: XP_022941082.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 550/563 (97.69%), Postives = 559/563 (99.29%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           M+KPLTRAVIF IWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MNKPLTRAVIFGIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSAT+L+DGKPSILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATILLDGKPSILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR
Sbjct: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           IIVGGEIGGQG+A+LYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 IIVGGEIGGQGLAVLYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
           L GLIKHVLKASFNNNDHYVLGRYTPE DTFSVETNFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 LDGLIKHVLKASFNNNDHYVLGRYTPETDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
           DKKRRILWGWILESDSE DDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV
Sbjct: 301 DKKRRILWGWILESDSEVDDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420
           SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLK+AEEMNTSWVDP+LLCKEKKASMNG
Sbjct: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKKAEEMNTSWVDPQLLCKEKKASMNG 420

Query: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480
           TIGPFGLLVLASK+LEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSL+EGLEKTTYGAFVD
Sbjct: 421 TIGPFGLLVLASKNLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLQEGLEKTTYGAFVD 480

Query: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540
           IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK
Sbjct: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540

Query: 541 LSGWSMKRAQIVPIQKRRKPSIT 564
           LSGWSMKRAQIVPIQKRRKPSIT
Sbjct: 541 LSGWSMKRAQIVPIQKRRKPSIT 563

[Close]
BLAST of CmaCh01G017330 vs. NCBI nr
Match: KAG6608372.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 530/542 (97.79%), Postives = 539/542 (99.45%), Query Frame = 0 

Query: 22  AASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLV 81
           +ASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLV
Sbjct: 26  SASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLV 85

Query: 82  DWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPL 141
           DWIHLEHAISPTEPYEANGCWSGSAT+L+DGKPSILYTGANSKNQQFQNLAVPKNPSDPL
Sbjct: 86  DWIHLEHAISPTEPYEANGCWSGSATILLDGKPSILYTGANSKNQQFQNLAVPKNPSDPL 145

Query: 142 LKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSEDF 201
           LKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQG+A+LYRSEDF
Sbjct: 146 LKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGLAVLYRSEDF 205

Query: 202 VNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLAGLIKHVLKASFNNNDHYVL 261
           VNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVL GLIKHVLKASFNNNDHYVL
Sbjct: 206 VNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLDGLIKHVLKASFNNNDHYVL 265

Query: 262 GRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSEADDI 321
           GRYTPE DTFSVETNFLSNG DMRYDYGKFYASKSFYDSDKKRRILWGWILESDSEADDI
Sbjct: 266 GRYTPETDTFSVETNFLSNGLDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSEADDI 325

Query: 322 SKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPGIMAA 381
           SKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPGIMAA
Sbjct: 326 SKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPGIMAA 385

Query: 382 QADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLEEQTAV 441
           QADIEVSFRLSGLK+AEEMNTSWVDP+LLCKEKKASMNGTIGPFGLLVLASK+LEEQTAV
Sbjct: 386 QADIEVSFRLSGLKKAEEMNTSWVDPQLLCKEKKASMNGTIGPFGLLVLASKNLEEQTAV 445

Query: 442 YFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLIDHSIVES 501
           YFRVFRKQYEKYVVLMCSDQSRSSL+EGLEKTTYGAFVDIDPVHETISLRSLIDHSIVES
Sbjct: 446 YFRVFRKQYEKYVVLMCSDQSRSSLQEGLEKTTYGAFVDIDPVHETISLRSLIDHSIVES 505

Query: 502 FGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQIVPIQKRRKPS 561
           FGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQIVPIQKRRKPS
Sbjct: 506 FGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQIVPIQKRRKPS 565

Query: 562 IT 564
           IT
Sbjct: 566 IT 567

[Close]
BLAST of CmaCh01G017330 vs. NCBI nr
Match: XP_038898883.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 1038.5 bits (2684), Expect = 2.1e-299
Identity = 483/562 (85.94%), Postives = 524/562 (93.24%), Query Frame = 0 

Query: 1   MSKPLTRAVIFCIWLAFNGVDAASNQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60
           M KPL   +  C WLA+NGVDA SNQPYRT FHFQPPKNWMNDPNGPMYYKGVYHLFYQY
Sbjct: 1   MKKPLIWVIPLCFWLAYNGVDAVSNQPYRTAFHFQPPKNWMNDPNGPMYYKGVYHLFYQY 60

Query: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTG 120
           NPYSAIWGNITWAHSISYDLVDWIHLEHA+SPTEPYE NGCWSGSAT+L+D KP+ILYTG
Sbjct: 61  NPYSAIWGNITWAHSISYDLVDWIHLEHALSPTEPYETNGCWSGSATILLDEKPAILYTG 120

Query: 121 ANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLISPVDDIDPLNFRDPTTAWLGPDRLWR 180
           ANSKNQQFQNLA+PKN SDPLLKEWIKSP NPLISPVDDI+PLNFRDPTTAWLGPDRLWR
Sbjct: 121 ANSKNQQFQNLALPKNESDPLLKEWIKSPQNPLISPVDDIEPLNFRDPTTAWLGPDRLWR 180

Query: 181 IIVGGEIGGQGVAILYRSEDFVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSV 240
           +I+GGEIGG+G+AILYRSEDFVNWTR K+PLH SN+TGMWECPDFYPVSINGSNGLDTSV
Sbjct: 181 VIIGGEIGGRGIAILYRSEDFVNWTRSKSPLHISNETGMWECPDFYPVSINGSNGLDTSV 240

Query: 241 LAGLIKHVLKASFNNNDHYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDS 300
             GLIKHVLKASFN+ DHYVLG Y P  DT+SVETNFLSNGSDMRYDYGKFYASKSFYDS
Sbjct: 241 QDGLIKHVLKASFNDADHYVLGSYIPGTDTYSVETNFLSNGSDMRYDYGKFYASKSFYDS 300

Query: 301 DKKRRILWGWILESDSEADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV 360
            KKRRILWGWI ESDSEADD +KGWSGLQSIPRSILL ESG+QL+ WPIKE+EKLRTKQV
Sbjct: 301 GKKRRILWGWIKESDSEADDTTKGWSGLQSIPRSILLGESGRQLVQWPIKELEKLRTKQV 360

Query: 361 SFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNG 420
           SFDD++LKSGSVFEVPG+ AAQADIE+SFRLSGLKEAEEMN SW+DP+LLCKE KA   G
Sbjct: 361 SFDDVNLKSGSVFEVPGLTAAQADIEISFRLSGLKEAEEMNVSWIDPQLLCKENKAVGKG 420

Query: 421 TIGPFGLLVLASKDLEEQTAVYFRVFRKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVD 480
           TIGPFGLLVLASKDL E TAVYFRVFRKQY+KYVVLMCSDQSRSSL+EGLEKTTYGAFVD
Sbjct: 421 TIGPFGLLVLASKDLTEHTAVYFRVFRKQYDKYVVLMCSDQSRSSLREGLEKTTYGAFVD 480

Query: 481 IDPVHETISLRSLIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISK 540
           +DP+ +TISLRSLIDHSI+ESFGG+GKACITARVYPK+AINQ+AHLYAFNNG+MDVR+S+
Sbjct: 481 VDPLRKTISLRSLIDHSIIESFGGDGKACITARVYPKIAINQEAHLYAFNNGSMDVRVSR 540

Query: 541 LSGWSMKRAQIVPIQKRRKPSI 563
           L+ WSMK+AQ+V IQKRRKPSI
Sbjct: 541 LNAWSMKKAQVVSIQKRRKPSI 562

[Close]
BLAST of CmaCh01G017330 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 674.5 bits (1739), Expect = 7.5e-194
Identity = 320/537 (59.59%), Postives = 407/537 (75.79%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLVDWI 84
           NQPYRTGFHFQPPKNWMNDPNGPM YKG+YHLFYQ+NP  A+WGNI WAHS S DL++W 
Sbjct: 48  NQPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWD 107

Query: 85  HLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLKE 144
               AI P+ P++ NGCWSGSAT+L +GKP ILYTG + KNQQ QN+A PKN SDP L+E
Sbjct: 108 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 167

Query: 145 WIKSPHNPLISP--VDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSEDFV 204
           W KSP NPL++P  V+ I+  +FRDPTTAWLG D+ WR+I+G +I  +G+AI Y S+DF+
Sbjct: 168 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 227

Query: 205 NWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLA---GLIKHVLKASFNN--ND 264
            W +   PLH  + +GMWECPDF+PV+  GSNG++TS       ++KHVLK S ++  +D
Sbjct: 228 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHD 287

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +G Y    D F  +  F  +G+  RYDYGK+YASK+F+DS K RRILWGW  ES S 
Sbjct: 288 YYTIGTYDRVKDKFVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSV 347

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV-SFDDMDLKSGSVFEVP 384
            DD+ KGWSG+Q+IPR I LD SGKQL+ WP++E+E+LRTKQV +  +  LKSGS  EV 
Sbjct: 348 EDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVY 407

Query: 385 GIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLE 444
           G+ AAQAD+EV F++  L++A+ +  SW DP+L+C +   S+   +GPFGL+VLASK+LE
Sbjct: 408 GVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLE 467

Query: 445 EQTAVYFRVF--RKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLI 504
           E T+VYFR+F  R+   KYVVLMCSDQSRSSLKE  +KTTYGAFVDI+P H+ +SLR+LI
Sbjct: 468 EYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALI 527

Query: 505 DHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQI 552
           DHS+VESFGG G+ACIT+RVYPKLAI + +HL+AFN G   V +  L+ WSM  AQI
Sbjct: 528 DHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 583

[Close]
BLAST of CmaCh01G017330 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 662.1 bits (1707), Expect = 3.9e-190
Identity = 317/537 (59.03%), Postives = 404/537 (75.23%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWGNITWAHSISYDLVDWI 84
           NQPYRTGFHFQPPKNWM   NGPM YKG+YHLFYQ+NP  A+WGNI WAHS S DL++W 
Sbjct: 48  NQPYRTGFHFQPPKNWM---NGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWD 107

Query: 85  HLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLKE 144
               AI P+ P++ NGCWSGSAT+L +GKP ILYTG + KNQQ QN+A PKN SDP L+E
Sbjct: 108 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 167

Query: 145 WIKSPHNPLISP--VDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSEDFV 204
           W KSP NPL++P  V+ I+  +FRDPTTAWLG D+ WR+I+G +I  +G+AI Y S+DF+
Sbjct: 168 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 227

Query: 205 NWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLA---GLIKHVLKASFNN--ND 264
            W +   PLH  + +GMWECPDF+PV+  GSNG++TS       ++KHVLK S ++  +D
Sbjct: 228 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHD 287

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +G Y    D F  +  F  +G+  RYDYGK+YASK+F+DS K RRILWGW  ES S 
Sbjct: 288 YYTIGTYDRVKDKFVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSV 347

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQV-SFDDMDLKSGSVFEVP 384
            DD+ KGWSG+Q+IPR I LD SGKQL+ WP++E+E+LRTKQV +  +  LKSGS  EV 
Sbjct: 348 EDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVY 407

Query: 385 GIMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLE 444
           G+ AAQAD+EV F++  L++A+ +  SW DP+L+C +   S+   +GPFGL+VLASK+LE
Sbjct: 408 GVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLE 467

Query: 445 EQTAVYFRVF--RKQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLI 504
           E T+VYFR+F  R+   KYVVLMCSDQSRSSLKE  +KTTYGAFVDI+P H+ +SLR+LI
Sbjct: 468 EYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALI 527

Query: 505 DHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQI 552
           DHS+VESFGG G+ACIT+RVYPKLAI + +HL+AFN G   V +  L+ WSM  AQI
Sbjct: 528 DHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 580

[Close]
BLAST of CmaCh01G017330 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 656.4 bits (1692), Expect = 2.1e-188
Identity = 318/539 (59.00%), Postives = 394/539 (73.10%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIWG-NITWAHSISYDLVDW 84
           NQPYRTG+HFQP KNWMNDPNGPM YKG+YHLFYQYNPY A+W   I W HS S DLV+W
Sbjct: 35  NQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNW 94

Query: 85  IHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLK 144
           I    A +P++P + NGCWSGS T+L +GKP ILYTG +    Q QN+AVP N SDP L+
Sbjct: 95  ISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLR 154

Query: 145 EWIKSPHNPLI--SPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGG-QGVAILYRSED 204
           EW K P NPL+  + V+ I+P  FRDPTTAWLG D  WR+IVG      +G+AILY+S D
Sbjct: 155 EWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRD 214

Query: 205 FVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDT-SVLAGLIKHVLKASF--NNND 264
           F NWT+   PLH  + TGMWECPDF+PVSI GS+G++T SV    IKHVLK S     +D
Sbjct: 215 FFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHD 274

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +G Y  E D +  +  F+ N S  R DYGK+YASK+FYD  KKRRILWGW+ ES   
Sbjct: 275 YYTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPA 334

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPG 384
            DDI KGWSGLQS PR I LDESGK+LL WPI+EIE LR +QV++    LK+GS  +V G
Sbjct: 335 KDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHG 394

Query: 385 IMAAQADIEVSFRLSGLKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLEE 444
           + AAQAD+EVSF++  L++A+ +  SW DP+ +C +   S+   +GPFGL+VLAS D+EE
Sbjct: 395 VTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEE 454

Query: 445 QTAVYFRVFRKQYE-----KYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRS 504
            T+VYFR+F+   +     KYVVLMCSDQSRSSL +  +K+T+GAFV IDP H+TISLR+
Sbjct: 455 YTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRT 514

Query: 505 LIDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQI 552
           LIDHSIVES+GG G+ CIT+RVYPKLAI ++A+L+ FN GT  V I  LS WS+K AQI
Sbjct: 515 LIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 573

[Close]
BLAST of CmaCh01G017330 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 615.1 bits (1585), Expect = 5.4e-176
Identity = 300/534 (56.18%), Postives = 387/534 (72.47%), Query Frame = 0 

Query: 25  NQPYRTGFHFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYSAIW--GNITWAHSISYDLVD 84
           NQPYRTG+HFQPPKNWM   NGPM YKG+YHLFYQ+N   A+       W H+ S DL++
Sbjct: 39  NQPYRTGYHFQPPKNWM---NGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLIN 98

Query: 85  WIHLEHAISPTEPYEANGCWSGSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLL 144
           WI L  AI P+ P + NGCWSGS T+L +GKP ILYTG +  N+Q QNL  PKN +DP L
Sbjct: 99  WITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYL 158

Query: 145 KEWIKSPHNPLI--SPVDDIDPLNFRDPTTAWLGPDRLWRIIVGGEIGGQGVAILYRSED 204
           + W KSP NPL+  SPV+ I+   FRDPTTAW G D  WRI  G + G +G+AIL+ S+D
Sbjct: 159 RHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKD 218

Query: 205 FVNWTRGKTPLHTSNKTGMWECPDFYPVSINGSNGLDTSVLAG-LIKHVLKASFNN--ND 264
           FV W +   PLH  + TGMWECPDF+PV+   S GLDTS  +G ++KHVLK S  +  ND
Sbjct: 219 FVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFND 278

Query: 265 HYVLGRYTPEMDTFSVETNFLSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSE 324
           +Y +G Y    D +  +  F+ + +  RYDYGKFYASK+FYDS  +RRILWGW+ ES  E
Sbjct: 279 YYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPE 338

Query: 325 ADDISKGWSGLQSIPRSILLDESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPG 384
            D+I KGW+GLQ+IPR + LDESGK+L+ WP+KEIE+LRT QV + +  LK GSV EV G
Sbjct: 339 KDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHG 398

Query: 385 IMAAQADIEVSFRLSG--LKEAEEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDL 444
           + A QAD+EV F++SG  L++A+ +   W DP+L+C ++ AS    +GPFGL+VLASK++
Sbjct: 399 VTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASSG--LGPFGLMVLASKNM 458

Query: 445 EEQTAVYFRVFR--KQYEKYVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSL 504
           EE T+V  R+FR  +  +++VV+MCSDQS SSL++G +KTTYGAF+DI P ++ ISLR+L
Sbjct: 459 EEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP-YQPISLRTL 518

Query: 505 IDHSIVESFGGNGKACITARVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMK 548
           ID SIVESFGG GK CIT+RVYPKLAI +  HL+AFN G+ +V +  LS WSMK
Sbjct: 519 IDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMK 566

[Close]
BLAST of CmaCh01G017330 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 610.9 bits (1574), Expect = 1.0e-174
Identity = 299/519 (57.61%), Postives = 375/519 (72.25%), Query Frame = 0 

Query: 45  NGPMYYKGVYHLFYQYNPYSAIWG-NITWAHSISYDLVDWIHLEHAISPTEPYEANGCWS 104
           +GPM YKG+YHLFYQYNPY A+W   I W HS S DLV+WI    A +P++P + NGCWS
Sbjct: 40  SGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWS 99

Query: 105 GSATVLVDGKPSILYTGANSKNQQFQNLAVPKNPSDPLLKEWIKSPHNPLI--SPVDDID 164
           GS T+L +GKP ILYTG +    Q QN+AVP N SDP L+EW K P NPL+  + V+ I+
Sbjct: 100 GSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGIN 159

Query: 165 PLNFRDPTTAWLGPDRLWRIIVGGEIGG-QGVAILYRSEDFVNWTRGKTPLHTSNKTGMW 224
           P  FRDPTTAWLG D  WR+IVG      +G+AILY+S DF NWT+   PLH  + TGMW
Sbjct: 160 PDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMW 219

Query: 225 ECPDFYPVSINGSNGLDT-SVLAGLIKHVLKASF--NNNDHYVLGRYTPEMDTFSVETNF 284
           ECPDF+PVSI GS+G++T SV    IKHVLK S     +D+Y +G Y  E D +  +  F
Sbjct: 220 ECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGF 279

Query: 285 LSNGSDMRYDYGKFYASKSFYDSDKKRRILWGWILESDSEADDISKGWSGLQSIPRSILL 344
           + N S  R DYGK+YASK+FYD  KKRRILWGW+ ES    DDI KGWSGLQS PR I L
Sbjct: 280 VQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWL 339

Query: 345 DESGKQLLLWPIKEIEKLRTKQVSFDDMDLKSGSVFEVPGIMAAQADIEVSFRLSGLKEA 404
           DESGK+LL WPI+EIE LR +QV++    LK+GS  +V G+ AAQAD+EVSF++  L++A
Sbjct: 340 DESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKA 399

Query: 405 EEMNTSWVDPELLCKEKKASMNGTIGPFGLLVLASKDLEEQTAVYFRVFRKQYE-----K 464
           + +  SW DP+ +C +   S+   +GPFGL+VLAS D+EE T+VYFR+F+   +     K
Sbjct: 400 DVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTK 459

Query: 465 YVVLMCSDQSRSSLKEGLEKTTYGAFVDIDPVHETISLRSLIDHSIVESFGGNGKACITA 524
           YVVLMCSDQSRSSL +  +K+T+GAFV IDP H+TISLR+LIDHSIVES+GG G+ CIT+
Sbjct: 460 YVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITS 519

Query: 525 RVYPKLAINQDAHLYAFNNGTMDVRISKLSGWSMKRAQI 552
           RVYPKLAI ++A+L+ FN GT  V I  LS WS+K AQI
Sbjct: 520 RVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 558

The following BLAST results are available for this feature:
BLAST of CmaCh01G017330 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q438661.1e-19259.59Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... [more]
Q67XZ33.0e-18759.00Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... [more]
Q70XE67.1e-18155.43Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1[more]
Q9LIB91.1e-17856.74Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... [more]
Q430895.3e-17656.56Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... [more]
BLAST of CmaCh01G017330 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1IZ330.0e+00100.00beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=... [more]
A0A6J1FLF00.0e+0097.69beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1CF862.0e-29585.61beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Momordica charantia OX=36... [more]
A0A1S3BVQ32.7e-29284.72beta-fructofuranosidase, insoluble isoenzyme CWINV1-like isoform X1 OS=Cucumis m... [more]
A0A0A0L0433.5e-29284.37Cell wall invertase 1 OS=Cucumis sativus OX=3659 GN=Csa_4G646140 PE=2 SV=1[more]
BLAST of CmaCh01G017330 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022982346.10.0e+00100.00beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita maxima][more]
XP_023524138.10.0e+0098.05beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. ... [more]
XP_022941082.10.0e+0097.69beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata][more]
KAG6608372.10.0e+0097.79Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosp... [more]
XP_038898883.12.1e-29985.94beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida][more]
BLAST of CmaCh01G017330 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G13790.17.5e-19459.59Glycosyl hydrolases family 32 protein [more]
AT3G13790.23.9e-19059.03Glycosyl hydrolases family 32 protein [more]
AT1G55120.12.1e-18859.00beta-fructofuranosidase 5 [more]
AT3G13784.15.4e-17656.18cell wall invertase 5 [more]
AT1G55120.31.0e-17457.61beta-fructofuranosidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 33..506
e-value: 1.8E-237
score: 804.9
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 33..348
e-value: 2.2E-100
score: 336.2
NoneNo IPR availableGENE3D2.60.120.560coord: 362..546
e-value: 8.1E-81
score: 272.2
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 22..551
NoneNo IPR availablePANTHERPTHR31953:SF48CELL WALL INVERTASE 1coord: 22..551
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 39..337
e-value: 2.36057E-170
score: 482.657
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 351..544
e-value: 1.5E-28
score: 99.7
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 37..361
e-value: 4.7E-116
score: 389.8
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 24..349
IPR018053Glycoside hydrolase, family 32, active sitePROSITEPS00609GLYCOSYL_HYDROL_F32coord: 33..46
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 348..548

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G017330.1CmaCh01G017330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds