CmaCh01G016510 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G016510
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbrassinosteroid-related acyltransferase 1
LocationCma_Chr01: 11298394 .. 11301133 (-)
RNA-Seq ExpressionCmaCh01G016510
SyntenyCmaCh01G016510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCACAAAATTCAGCTCTTCACAAAACATCATCATCATGGCCACTTCCCATGTTTCTGTTACAAAAACCCTCACTGTCTTTCCCAAAACCTTAAACTCTCCAAAACTCTTGGAGCTCTCAAACTTAGACAGGCAGTGTCCCTTGCACGTGTACCTTATTTTCTTCTACAAGCCATCTTCTAGCTCATCACTTGACTCGATCTTCACCAACCTGAAAATAGGGTTAGAAGAAACGCTATCAGTCTGGTCTCCGGCGGCCGGTCGGTTGGAGCCGAACCCCGCCAACGGTAAGCTGAATCTCCGGTGCAACAACCGTGGTGCAATTCTTGTTCAATCGTTCACCAATATCGAGATTTCAGAACTTGGAGATCTCTCGAATTACAATGAGTTCTTTGAGAAGTTGGTTTATAAACCTGTTTTGAATGGGAATTTCTCTGAGTTCCCTTTGGTTGTGGCTCAGGTGAGAGGTTTGTCTTTATAAAAAACAAAAGGGTTTTGGTTTTTTGTCAGAAAAAAGTGTTTTTTTGTTGAAAATTTTGCTTAATTTCATGAAAAAGTGAAAGGGAAAGAAAGGATTTTTATTGATTGGGACAAAGAAACAGAACATGTAAAAGGTTAGGGTTTAAGACAGCAGCTATGTCGAGGCTTTTTAGGGCAAAGAGACAAGCTTTGGATTAGACAATAATGAACACATTCTCACAGTCTGTAAAGACAATTATTTTTGTTCCTTCTTTCAAGATCTCCATAGATATGATCATATGATTCAAACCCAGTTGGCTTGAGATGAAAACAAGACAACTTACGTTGTTCGAAACAAGGTTTAATTACATGTTTGGTCGTTTGAATATTGTGTTTATTTAGCCCCTCGAGAGCAAAGTTAAAATTTTTATTCCTGATTGATGATATATGTCTTACCTAGCTAAACTATATATACTACCACCCTACCCTATCCTCAACAGGCAACTAGTCGTCCTACAGCTACTAGAAAAGGAACGGGAGTGATCTTGAATAGCAATCTTAAAGAATCTCCATAAAAATAGCACTAGATCGAGCATCGTTGATTTTGTAGCTGGTCAAAATCTTGGCTAATTGATCGAAGTAGGTAGTTTCAATAGACTCATAGATCCCGAAACAAATAGGACGGGTCGGCCCAAATTGACAAATTTCAATTATAACCCTAAAATTGTTAAGGTTTTTGAATTGGGGTCGGGAAAATTTAAGTTCGTTAAAGTATAGAGACTAAATAAACCGAAGAAGTTGTTGTTTTTGTTTATTTTAATGATGTTTTAAAAAGCAGGTGACAAGGTTTGGGTGTGGAGGATACTCCATAGGAACAGGAATAAGCCATTCATTGTTTGATGGACCAGCTGCGTACGAGTTCCTAAGTGCATGGGCGGCAAAGTCAGCCATTTTCAAGGACAAGAAGGAGCCATATGGGGTTGAGTTGCACACGCCAGTGCATGAGAGAGGGAGGCTTTTGATGGATTACGACGCGAAACGGAGCTCGGAGAAGAGGGCGATGCCGGTGTCGGTGTCGGGGGCGGTAGCGATCGATCATTTGTATCGGTTGATAATGCAAAGCGTGACGGGGAAGGAGGAAATGGGGAAAAAAGAATATGTTTATAGAACGTTTAGATTGAGTAGAGGAATGATCGAGAGATTGAAGAAGAACGCTATGGGAGAAAATGGTGGCTTTGCTTGTTCATCCTTCGATGTTATTGCTGCTCATCTATGGAAGGTACTAAACTTACATTTTTTTTTTTTTAATGAGTATAATATCTACTAAGGGTTAAGAGTTTAGGGTATCAGAGCCAGACACTAGGAAACTAAGCATTCTATATAAAAGGTGTGGAAACCTCTCCATAGCATACGTGTTATAAAACCGTGAGGCTGACGGTGATATGTAACGGGTCAAAGCTGACAAGTAGTTGTTACAGTTATTTCCTATATAAATATGGGTTTGACTTTTTTTTAAAAAAAAAACATTCAATCTTGGTCTTTAAATTTGGTTGAGACATATTATTGTCTGTTACAGGCAAGAACTAAGGCTATGTCTGTGAGCAATGAAAAGATGGTGTGCTTACAATTTGCAGTGAACGCCAGGAACAAACTGATGCCTCCTCTACCTAAAGGCTTCAGCGGCAATGCCTTCGTGCTCGCTTCGGTCGCCTTAACCGCAAGACAATTGGAGGACGAAAGCTACAAAACAATAGTCGAGATGATACGAAACGCCAAGTCTCGAGTCGACAACGACTACATCAATGCATACAAACAAGGCCTCGAAGGCCCTCAGGCTTCCCTTCCTCCACTCAAGGAGCTCACTGTTGTATCGGATTGGACCCGAATGCCATTCCACAAGATCGAGTTCTTACATGGACAAGCTCTCTATGCATCTCCTTTAAAGCCACCGATCCTCGACGTCGCTTACTTTCTGCAGAGCCCCCTAGATAGTCAAGCAATCGATGTTCGGGTCCCTTTGCTGGAAGGGACTCTTGAGGCTTTCTCCTCCTACTTCTTGACCGGCATGCAATGAGGCTTTAAAGTTACCCTTCTTTTGCAGGGTTGCTTTGTTTTAGGGTATCCCTCTAGTAAGGTTCTAAGTTTGAATAGCATAAGGGTTTGTTCATTACTGAGCAAATGGTCAAATAGTGTACTAAGAAGAATAATATGATATCTTCGTAGAATAAGAGAGGCGACAAGCTTTTGTGGTACGGTACAGTCAATGGACAGTCCGATGGGCG

mRNA sequence

ACCACAAAATTCAGCTCTTCACAAAACATCATCATCATGGCCACTTCCCATGTTTCTGTTACAAAAACCCTCACTGTCTTTCCCAAAACCTTAAACTCTCCAAAACTCTTGGAGCTCTCAAACTTAGACAGGCAGTGTCCCTTGCACGTGTACCTTATTTTCTTCTACAAGCCATCTTCTAGCTCATCACTTGACTCGATCTTCACCAACCTGAAAATAGGGTTAGAAGAAACGCTATCAGTCTGGTCTCCGGCGGCCGGTCGGTTGGAGCCGAACCCCGCCAACGGTAAGCTGAATCTCCGGTGCAACAACCGTGGTGCAATTCTTGTTCAATCGTTCACCAATATCGAGATTTCAGAACTTGGAGATCTCTCGAATTACAATGAGTTCTTTGAGAAGTTGGTTTATAAACCTGTTTTGAATGGGAATTTCTCTGAGTTCCCTTTGGTTGTGGCTCAGGTGACAAGGTTTGGGTGTGGAGGATACTCCATAGGAACAGGAATAAGCCATTCATTGTTTGATGGACCAGCTGCGTACGAGTTCCTAAGTGCATGGGCGGCAAAGTCAGCCATTTTCAAGGACAAGAAGGAGCCATATGGGGTTGAGTTGCACACGCCAGTGCATGAGAGAGGGAGGCTTTTGATGGATTACGACGCGAAACGGAGCTCGGAGAAGAGGGCGATGCCGGTGTCGGTGTCGGGGGCGGTAGCGATCGATCATTTGTATCGGTTGATAATGCAAAGCGTGACGGGGAAGGAGGAAATGGGGAAAAAAGAATATGTTTATAGAACGTTTAGATTGAGTAGAGGAATGATCGAGAGATTGAAGAAGAACGCTATGGGAGAAAATGGTGGCTTTGCTTGTTCATCCTTCGATGTTATTGCTGCTCATCTATGGAAGGCAAGAACTAAGGCTATGTCTGTGAGCAATGAAAAGATGGTGTGCTTACAATTTGCAGTGAACGCCAGGAACAAACTGATGCCTCCTCTACCTAAAGGCTTCAGCGGCAATGCCTTCGTGCTCGCTTCGGTCGCCTTAACCGCAAGACAATTGGAGGACGAAAGCTACAAAACAATAGTCGAGATGATACGAAACGCCAAGTCTCGAGTCGACAACGACTACATCAATGCATACAAACAAGGCCTCGAAGGCCCTCAGGCTTCCCTTCCTCCACTCAAGGAGCTCACTGTTGTATCGGATTGGACCCGAATGCCATTCCACAAGATCGAGTTCTTACATGGACAAGCTCTCTATGCATCTCCTTTAAAGCCACCGATCCTCGACGTCGCTTACTTTCTGCAGAGCCCCCTAGATAGTCAAGCAATCGATGTTCGGGTCCCTTTGCTGGAAGGGACTCTTGAGGCTTTCTCCTCCTACTTCTTGACCGGCATGCAATGAGGCTTTAAAGTTACCCTTCTTTTGCAGGGTTGCTTTGTTTTAGGGTATCCCTCTAGTAAGGTTCTAAGTTTGAATAGCATAAGGGTTTGTTCATTACTGAGCAAATGGTCAAATAGTGTACTAAGAAGAATAATATGATATCTTCGTAGAATAAGAGAGGCGACAAGCTTTTGTGGTACGGTACAGTCAATGGACAGTCCGATGGGCG

Coding sequence (CDS)

ATGGCCACTTCCCATGTTTCTGTTACAAAAACCCTCACTGTCTTTCCCAAAACCTTAAACTCTCCAAAACTCTTGGAGCTCTCAAACTTAGACAGGCAGTGTCCCTTGCACGTGTACCTTATTTTCTTCTACAAGCCATCTTCTAGCTCATCACTTGACTCGATCTTCACCAACCTGAAAATAGGGTTAGAAGAAACGCTATCAGTCTGGTCTCCGGCGGCCGGTCGGTTGGAGCCGAACCCCGCCAACGGTAAGCTGAATCTCCGGTGCAACAACCGTGGTGCAATTCTTGTTCAATCGTTCACCAATATCGAGATTTCAGAACTTGGAGATCTCTCGAATTACAATGAGTTCTTTGAGAAGTTGGTTTATAAACCTGTTTTGAATGGGAATTTCTCTGAGTTCCCTTTGGTTGTGGCTCAGGTGACAAGGTTTGGGTGTGGAGGATACTCCATAGGAACAGGAATAAGCCATTCATTGTTTGATGGACCAGCTGCGTACGAGTTCCTAAGTGCATGGGCGGCAAAGTCAGCCATTTTCAAGGACAAGAAGGAGCCATATGGGGTTGAGTTGCACACGCCAGTGCATGAGAGAGGGAGGCTTTTGATGGATTACGACGCGAAACGGAGCTCGGAGAAGAGGGCGATGCCGGTGTCGGTGTCGGGGGCGGTAGCGATCGATCATTTGTATCGGTTGATAATGCAAAGCGTGACGGGGAAGGAGGAAATGGGGAAAAAAGAATATGTTTATAGAACGTTTAGATTGAGTAGAGGAATGATCGAGAGATTGAAGAAGAACGCTATGGGAGAAAATGGTGGCTTTGCTTGTTCATCCTTCGATGTTATTGCTGCTCATCTATGGAAGGCAAGAACTAAGGCTATGTCTGTGAGCAATGAAAAGATGGTGTGCTTACAATTTGCAGTGAACGCCAGGAACAAACTGATGCCTCCTCTACCTAAAGGCTTCAGCGGCAATGCCTTCGTGCTCGCTTCGGTCGCCTTAACCGCAAGACAATTGGAGGACGAAAGCTACAAAACAATAGTCGAGATGATACGAAACGCCAAGTCTCGAGTCGACAACGACTACATCAATGCATACAAACAAGGCCTCGAAGGCCCTCAGGCTTCCCTTCCTCCACTCAAGGAGCTCACTGTTGTATCGGATTGGACCCGAATGCCATTCCACAAGATCGAGTTCTTACATGGACAAGCTCTCTATGCATCTCCTTTAAAGCCACCGATCCTCGACGTCGCTTACTTTCTGCAGAGCCCCCTAGATAGTCAAGCAATCGATGTTCGGGTCCCTTTGCTGGAAGGGACTCTTGAGGCTTTCTCCTCCTACTTCTTGACCGGCATGCAATGA

Protein sequence

MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ
Homology
BLAST of CmaCh01G016510 vs. ExPASy Swiss-Prot
Match: Q9SZ58 (Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT1 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 9.0e-118
Identity = 230/469 (49.04%), Postives = 318/469 (67.80%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLN--SPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTN 60
           +  + + + + L V+P+  N    KL+ LSNLDRQCPL +Y +FFYK +++   DS+F+N
Sbjct: 4   LMATRIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNTTTRDFDSVFSN 63

Query: 61  LKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSF-TNIEISELGDLSNYNE 120
           LK+GLEET+SVW PAAGRL  +    KLN+RCN+ GA++V++  T +++SELGDL+ YNE
Sbjct: 64  LKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQYNE 123

Query: 121 FFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKS 180
           F+E LVYKP L+G+FS  PLVVAQVTRF CGGYSIG G SHSLFDG +AYEF+ AWA+ S
Sbjct: 124 FYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWASNS 183

Query: 181 AI-------FKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLY 240
            I         +KKE   ++   PVH+R  LL++ DA R           + A AI HLY
Sbjct: 184 HIHNKSNSKITNKKEDVVIK---PVHDRRNLLVNRDAVRE----------TNAAAICHLY 243

Query: 241 RLIMQSVTGKEEMGK------KEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAA 300
           +LI Q++   +E  +        +V +TF L+   IE +KK ++    GF CSSF+ +AA
Sbjct: 244 QLIKQAMMTYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSL---EGFMCSSFEFLAA 303

Query: 301 HLWKARTKAMSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQ-LEDES 360
           HLWKART+A+ +  + MVCLQFAV+ R +   PLP+GFSGNA+VLASVA TAR+ LE+ +
Sbjct: 304 HLWKARTRALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELT 363

Query: 361 YKTIVEMIRNAKSRVDNDYINAYKQGLEGP-QASLPPLKELTVVSDWTRMPFHKIEFLHG 420
            ++IV  IR AK  +D  YIN+Y + L G    +LPPLKELT++SDWT+MPFH + F +G
Sbjct: 364 LESIVNKIREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNG 423

Query: 421 --QALYASPLKPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFL 450
              A Y +PL PP+  VAYF+++P D++ + VR+ L    +  FS++FL
Sbjct: 424 GEPADYMAPLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHFL 456

BLAST of CmaCh01G016510 vs. ExPASy Swiss-Prot
Match: Q94CD1 (Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN=HHT1 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-44
Identity = 128/441 (29.02%), Postives = 215/441 (48.75%), Query Frame = 0

Query: 14  VFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLKIGLEETLSVWSPA 73
           V P++     L  LSNLD+   + V  I+ +K     + +++   +K  L + L  + P 
Sbjct: 41  VKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQV-IKKALSQVLVHYYPL 100

Query: 74  AGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYN-EFFEKLVYKPVLNGNF 133
           AGRL  +P  GKL + C   G + V++  N ++ E+GD++  + E   KLVY  V   N 
Sbjct: 101 AGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDAKNI 160

Query: 134 SEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFKDKKEPYGVELH 193
            E P V AQVT+F CGG+ +G  ++H +FDG  A EF+++W   +          G+ L 
Sbjct: 161 LEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVAR---------GLPLT 220

Query: 194 TPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKEEMGKKEYVYRT 253
           TP      +L   +  +          +     I+ LY              K+  +YR+
Sbjct: 221 TPPFSDRTILNARNPPKIENLHQEFEEIEDKSNINSLYT-------------KEPTLYRS 280

Query: 254 FRLSRGMIERLKKNAMGENG----GFACSSFDVIAAHLWKARTKAMSVSNEKMVCLQFAV 313
           F      I++LK  A  EN     G +C+SF+ ++A +W+ARTK++ + +++   L FAV
Sbjct: 281 FCFDPEKIKKLKLQAT-ENSESLLGNSCTSFEALSAFVWRARTKSLKMLSDQKTKLLFAV 340

Query: 314 NARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDNDYINAYKQ 373
           + R K  P LPKG+ GN  VL +    A +L ++     V ++R A   V + Y+ +   
Sbjct: 341 DGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAID 400

Query: 374 GLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYFLQSPLDSQ 433
             E  +A  P L    +++ W+R+ FH  +F  G+ + + P+  P  +V  FL      +
Sbjct: 401 YFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRR 455

Query: 434 AIDVRVPLLEGTLEAFSSYFL 450
           +I+V + L    ++ F   FL
Sbjct: 461 SINVLLGLPATAMDVFQEQFL 455

BLAST of CmaCh01G016510 vs. ExPASy Swiss-Prot
Match: Q9FFQ7 (Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FACT PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 9.8e-40
Identity = 125/443 (28.22%), Postives = 216/443 (48.76%), Query Frame = 0

Query: 6   VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLKIGLEE 65
           V+  + + V P +     L  LSNLD+   + V   +++K +S S+ +S +  +K  L E
Sbjct: 9   VTRKEPVLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNSRSNEES-YEVIKKSLSE 68

Query: 66  TLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDL---SNYNEFFEKL 125
            L  + PAAGRL  +P  GK+ + C   G ++V++  N  I ++       +  E  EKL
Sbjct: 69  VLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETLEKL 128

Query: 126 VYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFKD 185
           VY      N  E P VV QVT F CGG+ +G G++H++FDG AA EFL++WA  +     
Sbjct: 129 VYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETAR---- 188

Query: 186 KKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKEE 245
                G+ L  P      LL      R+  K   P          H     ++ ++G  +
Sbjct: 189 -----GLPLSVPPFLDRTLL----RPRTPPKIEFP----------HNEFEDLEDISGTGK 248

Query: 246 M-GKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEKM 305
           +   ++ VY++F      +ERLK   M E      ++F  +   LW+AR +A+ +  ++ 
Sbjct: 249 LYSDEKLVYKSFLFGPEKLERLK--IMAET---RSTTFQTLTGFLWRARCQALGLKPDQR 308

Query: 306 VCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDND 365
           + L FA + R++ +P LPKG+SGN  V      TA ++        V +++ A   V++ 
Sbjct: 309 IKLLFAADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDG 368

Query: 366 YINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYFL 425
           ++ +     E  +A  P L    +++ W ++ FH  +F  G+ + + P+  P  +V  FL
Sbjct: 369 FMRSAIDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFL 420

Query: 426 QSPLDSQAIDVRVPLLEGTLEAF 445
               D+++I+V + L    ++ F
Sbjct: 429 PCGSDTKSINVLLGLPGSAMKVF 420

BLAST of CmaCh01G016510 vs. ExPASy Swiss-Prot
Match: A0PDV5 (Rosmarinate synthase OS=Plectranthus scutellarioides OX=4142 GN=RAS PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.1e-33
Identity = 129/460 (28.04%), Postives = 198/460 (43.04%), Query Frame = 0

Query: 6   VSVTKTLTVFPKTLNSPKLLELSNLDRQCP--LHVYLIFFYKPSSSSSLDSIF--TNLKI 65
           + V  +  + P        L LSNLD   P   H   + FY    S   D+ F    LK 
Sbjct: 3   IEVKDSTMIKPSAETPGGSLWLSNLDLLSPANYHTLSVHFYSHDGS---DNFFDAAGLKE 62

Query: 66  GLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFEK 125
            L   L  + P AGRL+ N    +L + CNN G +LV++  +  + ELGD +   E    
Sbjct: 63  SLSRALVEFYPYAGRLKLN--GNRLEIDCNNEGLLLVEAECDGALDELGDFAPRPEL--N 122

Query: 126 LVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFK 185
           L+ K   +   S +PL+V Q+TRF CGG ++G    H L DG AA  F++ WA  S    
Sbjct: 123 LIPKVDYSRGISTYPLMVFQLTRFKCGGVALGVANEHHLSDGVAALHFINTWAHLSR--- 182

Query: 186 DKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKE 245
                 G    TP       L  +D    S +       S A              T + 
Sbjct: 183 ------GAPAPTP-------LPHFDRSSLSARNPPQPQFSHAE--------YQPPPTLEN 242

Query: 246 EMGKKEYVYRTFRLSRGMIERLK---KNAMGENG-GFACSSFDVIAAHLWKARTKAMSVS 305
            +   +  +  F+L+R  +  LK   K A  + G G + S+F+V+A H+W++   A  + 
Sbjct: 243 PLPHTDIAHSRFKLTRDQLNSLKSKFKTAPADGGAGKSYSTFEVLAGHIWRSVCIARGLP 302

Query: 306 NEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSR 365
             +   L    + R +L   LP GF GNA   A+   T  ++E  S    V  + +  SR
Sbjct: 303 EGQETKLHIPFDGRGRLQ--LPPGFFGNAIFFATPIATCGEIESNSLNYAVRRVSDGVSR 362

Query: 366 VDNDYINAYKQGLEGPQASLPPLKE---------LTVVSDWTRMPFHKIEFLHGQALYAS 425
           +D DY+ +    LE  Q  +  L +         L V+S W  +P ++ +F  G+A+Y  
Sbjct: 363 LDEDYLRSSIDFLE-LQEDISKLAQGAHSFRCPNLWVIS-WVWLPIYEPDFGWGKAVYMG 422

Query: 426 PLKPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYF 449
           P   P    +Y L +P    ++ V + L +  +E F   F
Sbjct: 423 PWAAPFEGKSYLLPNPEKDGSLFVSITLHKQHMERFEKLF 427

BLAST of CmaCh01G016510 vs. ExPASy Swiss-Prot
Match: Q8S9G6 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=29808 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 9.8e-32
Identity = 110/414 (26.57%), Postives = 186/414 (44.93%), Query Frame = 0

Query: 5   HVSVTKTLTVFPKTLNSPKLLELSNLD-----RQCPLHVYLIFFYKPSSSSSLDSIFTNL 64
           HV++ + + V P        L+LS++D     R    +  LI+   PS +         +
Sbjct: 7   HVNLNEKVMVGPSLPLPKTTLQLSSIDNLPGVRGSIFNALLIYNASPSPTMVSADPAKLI 66

Query: 65  KIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFF 124
           +  L + L  + P AGRL     NG L + C   GA+ +++  + E+S LGD  + N  F
Sbjct: 67  REALAKILVYYPPFAGRLR-ETENGDLEVECTGEGAMFLEAMADNELSVLGDFDDSNPSF 126

Query: 125 EKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAI 184
           ++L++   L+ NF + PL+V QVTRF CGG+ +G    H + DG  A +FL   A  +  
Sbjct: 127 QQLLFSLPLDTNFKDLPLLVVQVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLAEMA-- 186

Query: 185 FKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTG 244
                             RG + +  +   + E   + +     +   H   L   S+  
Sbjct: 187 ------------------RGEVKLSLEPIWNRE--LVKLDDPKYLQFFHFEFLRAPSIVE 246

Query: 245 KEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNE 304
           K        V   F +    I  +K++ M E   F CSSF+V +A  W ART+A  +   
Sbjct: 247 K-------IVQTYFIIDFETINYIKQSVMEECKEF-CSSFEVASAMTWIARTRAFQIPES 306

Query: 305 KMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVD 364
           + V + F ++ RN   PPLP G+ GN+   A      + L   S    + +I+ +K  ++
Sbjct: 307 EYVKILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKVSLN 366

Query: 365 NDYINAYKQGLEGPQASLPPLKELTVV--SDWTRMPFHKIEFLHGQALYASPLK 412
           +   N   + +  P      +    VV  +DW+R+ F +++F  G A+  SP++
Sbjct: 367 D---NFKSRAVVKPSELDVNMNHENVVAFADWSRLGFDEVDFGWGNAVSVSPVQ 386

BLAST of CmaCh01G016510 vs. ExPASy TrEMBL
Match: A0A6J1IT61 (brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC111480377 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.4e-259
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60
           MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK
Sbjct: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60

Query: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120
           IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE
Sbjct: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120

Query: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180
           KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF
Sbjct: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180

Query: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240
           KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK
Sbjct: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240

Query: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300
           EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK
Sbjct: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300

Query: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360
           MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN
Sbjct: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360

Query: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420
           DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF
Sbjct: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420

Query: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ
Sbjct: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 453

BLAST of CmaCh01G016510 vs. ExPASy TrEMBL
Match: A0A6J1FK90 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446510 PE=3 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.2e-251
Identity = 439/453 (96.91%), Postives = 445/453 (98.23%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60
           MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSS DS+FT LK
Sbjct: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSFDSVFTKLK 60

Query: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120
           IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTN+EIS+LGDLSNYNEFFE
Sbjct: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNVEISKLGDLSNYNEFFE 120

Query: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180
           KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF
Sbjct: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180

Query: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240
           KDKKEPYGVELHTPVHERGRLLM YDAKRS EKRAMP  VSGAVAIDHLYRLIMQS+TGK
Sbjct: 181 KDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMP--VSGAVAIDHLYRLIMQSMTGK 240

Query: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300
           EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS EK
Sbjct: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSKEK 300

Query: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360
           MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIV+MIRNAKSRVDN
Sbjct: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIRNAKSRVDN 360

Query: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420
           DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF
Sbjct: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420

Query: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           LQSP+DSQAIDVRVPLL GTLEAFSSYFLTGMQ
Sbjct: 421 LQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of CmaCh01G016510 vs. ExPASy TrEMBL
Match: A0A6J1F9T5 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443397 PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.6e-208
Identity = 367/455 (80.66%), Postives = 405/455 (89.01%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSS---SSSLDSIFT 60
           MAT HVS+TK+LTVFPKTLN  KLLELSNLDRQCPLH+YLIFFY PSS   +SSLDS+FT
Sbjct: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60

Query: 61  NLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNE 120
            LKIGLEETLSVWSPAAGRLEPNPA+GKLNLRC+NRGAIL ++ T+++ISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKS 180
           FFE+LV+KPVL+G+F EFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA KS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSV 240
           AIFKD KE YGVEL  PVHERGRLLM     R S K +   S +GAVAIDHLY+LIMQS 
Sbjct: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKPSAAGSGAGAVAIDHLYKLIMQSA 240

Query: 241 TGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS 300
            G EEM KKEY  RT R+SRGMIE LKK A GENGGF CSSFDVIAAHLWKARTKA+SVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKAFGENGGFPCSSFDVIAAHLWKARTKALSVS 300

Query: 301 NEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSR 360
            EK+VCLQFAV+ARNKL PPLPKGFSGN+FVLASVAL A+QLE+ESYKTI+EMIR+AKS+
Sbjct: 301 KEKIVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDV 420
           VD+DY+NAY+ GL+GPQASLPPLKELTV+SDWTRMPFHKIEFLHG+A YASPLKPPILDV
Sbjct: 361 VDDDYVNAYQHGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

Query: 421 AYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGM 453
           AYFLQ+P DSQAIDVRV LL  TL+AFS+YF T M
Sbjct: 421 AYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFFTSM 455

BLAST of CmaCh01G016510 vs. ExPASy TrEMBL
Match: A0A6J1IG59 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476630 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.1e-206
Identity = 366/456 (80.26%), Postives = 403/456 (88.38%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSS---SSSLDSIFT 60
           MAT HVS T +LTVFPKTLN  KLLELSNLDRQCPLH+YLIFFY PSS   +SSLDS+FT
Sbjct: 1   MATPHVSRTTSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSAYTNSSLDSVFT 60

Query: 61  NLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNE 120
            LKIGLEETLSVWSPAAGRLEPNPA+GKLNLRC+NRGAIL ++ T+++ISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKS 180
           FFE+LV+KPVL+G+F EFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA KS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSV 240
           AIFKD KE   VEL  PVHERGRLLM     R S KR+   S + AVAIDHLY+LIMQS 
Sbjct: 181 AIFKDNKEQNEVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAEAVAIDHLYKLIMQSA 240

Query: 241 TGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS 300
            G EEM KKEY  RT R+SRGMIERLKK A GENGGFACSSFDVIAAHLWKARTKA+SVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIERLKKKAFGENGGFACSSFDVIAAHLWKARTKALSVS 300

Query: 301 NEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSR 360
            EK VCLQFAV+ARNKL PPLPKGFSGN+FVLASVAL A+QLE+ESYKTI+EMIR+AKS+
Sbjct: 301 KEKTVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDV 420
           VD+DY+NAY+ GL+GPQASLPPL ELTV+SDWTRMPFHKIEFLHG+A YASPLKPPILDV
Sbjct: 361 VDDDYVNAYQLGLDGPQASLPPLNELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

Query: 421 AYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           AYFLQ+P DSQAIDVRV LL  TL+AFS+YFLT +Q
Sbjct: 421 AYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFLTSIQ 456

BLAST of CmaCh01G016510 vs. ExPASy TrEMBL
Match: A0A1S3BW29 (omega-hydroxypalmitate O-feruloyl transferase OS=Cucumis melo OX=3656 GN=LOC103493765 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 1.1e-198
Identity = 358/458 (78.17%), Postives = 397/458 (86.68%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSS---SSSLDSIFT 60
           MA   VS+TK+L V PKT N PK LELSNLDRQCPLH+YLIFFY PSS   +SSLD +F 
Sbjct: 1   MANPQVSLTKSLIVIPKTSNPPKFLELSNLDRQCPLHMYLIFFYNPSSICTNSSLDLVFN 60

Query: 61  NLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNE 120
            LKIGLEETLS+WSPAAGRL+ NP +GKLNLRC+NRGAILVQ+ TN++ISELGDLSNYN+
Sbjct: 61  KLKIGLEETLSIWSPAAGRLDRNPTDGKLNLRCDNRGAILVQAITNVKISELGDLSNYND 120

Query: 121 FFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKS 180
           FFEKLV KPVLNGNFSEFPLVVAQVTRFGCGGY+IGTGISHSLFDGPAAY+FLSAWA  S
Sbjct: 121 FFEKLVQKPVLNGNFSEFPLVVAQVTRFGCGGYAIGTGISHSLFDGPAAYDFLSAWATNS 180

Query: 181 AIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSV 240
           AIFKD  + Y ++  +PVHERGRLL    A R           SG VAIDHLY+LIMQS 
Sbjct: 181 AIFKD--DNYKMQTISPVHERGRLLTGTVANR----------CSGTVAIDHLYKLIMQSA 240

Query: 241 TGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGE-NGG-FACSSFDVIAAHLWKARTKAMS 300
            G EE+G+KEYV RTFR+S+ MIERLK NA+GE NGG FACSSFD+IAAHLWKARTKA+S
Sbjct: 241 MGNEEVGRKEYVCRTFRVSKEMIERLKMNALGEINGGAFACSSFDLIAAHLWKARTKALS 300

Query: 301 VSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAK 360
           VS EKMVCLQFAV+ARNKLMPPLPKGFSGNAFVLASVALTA+QL++ESYKTI+EMI+ AK
Sbjct: 301 VSKEKMVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLDEESYKTIIEMIQKAK 360

Query: 361 SRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPIL 420
           SRVDNDYINAY+QGLEGPQASLPPLKELTVVSDWTRMPFHKI+FLHG+A YASPLKPPIL
Sbjct: 361 SRVDNDYINAYQQGLEGPQASLPPLKELTVVSDWTRMPFHKIQFLHGEAAYASPLKPPIL 420

Query: 421 DVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           +VAYFLQ+P D QAIDV VPLLE T +AFSSYFLT MQ
Sbjct: 421 EVAYFLQNPTDGQAIDVHVPLLEHTFDAFSSYFLTRMQ 446

BLAST of CmaCh01G016510 vs. NCBI nr
Match: XP_022981142.1 (brassinosteroid-related acyltransferase 1 [Cucurbita maxima])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60
           MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK
Sbjct: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60

Query: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120
           IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE
Sbjct: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120

Query: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180
           KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF
Sbjct: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180

Query: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240
           KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK
Sbjct: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240

Query: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300
           EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK
Sbjct: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300

Query: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360
           MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN
Sbjct: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360

Query: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420
           DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF
Sbjct: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420

Query: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ
Sbjct: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 453

BLAST of CmaCh01G016510 vs. NCBI nr
Match: XP_022941116.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 876.7 bits (2264), Expect = 8.6e-251
Identity = 439/453 (96.91%), Postives = 445/453 (98.23%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60
           MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSS DS+FT LK
Sbjct: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSFDSVFTKLK 60

Query: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120
           IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTN+EIS+LGDLSNYNEFFE
Sbjct: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNVEISKLGDLSNYNEFFE 120

Query: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180
           KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF
Sbjct: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180

Query: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240
           KDKKEPYGVELHTPVHERGRLLM YDAKRS EKRAMP  VSGAVAIDHLYRLIMQS+TGK
Sbjct: 181 KDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMP--VSGAVAIDHLYRLIMQSMTGK 240

Query: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300
           EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS EK
Sbjct: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSKEK 300

Query: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360
           MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIV+MIRNAKSRVDN
Sbjct: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIRNAKSRVDN 360

Query: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420
           DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF
Sbjct: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420

Query: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           LQSP+DSQAIDVRVPLL GTLEAFSSYFLTGMQ
Sbjct: 421 LQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of CmaCh01G016510 vs. NCBI nr
Match: KAG6608286.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037638.1 Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 875.5 bits (2261), Expect = 1.9e-250
Identity = 438/453 (96.69%), Postives = 445/453 (98.23%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60
           MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSS DS+FT LK
Sbjct: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSFDSVFTKLK 60

Query: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120
           +GLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILV+SFTN+EISELGDLSNYNEFFE
Sbjct: 61  MGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVRSFTNVEISELGDLSNYNEFFE 120

Query: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180
           KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF
Sbjct: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180

Query: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240
           KDKKEPYGVELHTPVHERGRLLM YDAKRS EKRAMP  VSGAVAIDHLYRLIMQS+TGK
Sbjct: 181 KDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMP--VSGAVAIDHLYRLIMQSMTGK 240

Query: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300
           EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS EK
Sbjct: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSKEK 300

Query: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360
           MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIV+MIRNAKSRVDN
Sbjct: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIRNAKSRVDN 360

Query: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420
           DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF
Sbjct: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420

Query: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           LQSP+DSQAIDVRVPLL GTLEAFSSYFLTGMQ
Sbjct: 421 LQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of CmaCh01G016510 vs. NCBI nr
Match: XP_023524200.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874.0 bits (2257), Expect = 5.6e-250
Identity = 438/453 (96.69%), Postives = 444/453 (98.01%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLK 60
           MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDS+FTNLK
Sbjct: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSVFTNLK 60

Query: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 120
           IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFT++EISELGDLSNYNEFFE
Sbjct: 61  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTHVEISELGDLSNYNEFFE 120

Query: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180
           KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF
Sbjct: 121 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 180

Query: 181 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 240
           KDK EPYGVELHTPVHERGRLLM YD KRS EKRAMP  +SGAVAIDHLYRLIMQS+TGK
Sbjct: 181 KDKNEPYGVELHTPVHERGRLLMGYDTKRSLEKRAMP--LSGAVAIDHLYRLIMQSMTGK 240

Query: 241 EEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEK 300
           EE+GKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS EK
Sbjct: 241 EEIGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSKEK 300

Query: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360
           MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN
Sbjct: 301 MVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDN 360

Query: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420
           DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF
Sbjct: 361 DYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYF 420

Query: 421 LQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           LQSP DSQAIDVRVPLL GTLEAFSSYFLTGMQ
Sbjct: 421 LQSPTDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of CmaCh01G016510 vs. NCBI nr
Match: KAG6591543.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 738.8 bits (1906), Expect = 2.8e-209
Identity = 369/456 (80.92%), Postives = 406/456 (89.04%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSS---SSSLDSIFT 60
           MAT HVS+TK+LTVFPKTLN  KLLELSNLDRQCPLH+YLIFFY PSS   +SSLDS+FT
Sbjct: 1   MATPHVSLTKSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSIYTNSSLDSVFT 60

Query: 61  NLKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNE 120
            LKIGLEETLSVWSPAAGRLEPNPA+GKLNLRC+NRGAIL ++ T+++ISELGDLSNYNE
Sbjct: 61  KLKIGLEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNE 120

Query: 121 FFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKS 180
           FFE+LV+KPVL+G+F EFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWA KS
Sbjct: 121 FFEELVHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKS 180

Query: 181 AIFKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSV 240
           AIFKD KE YGVEL  PVHERGRLLM     R S KR+   S +GAVAIDHLY+LIMQS 
Sbjct: 181 AIFKDDKEQYGVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAGAVAIDHLYKLIMQSA 240

Query: 241 TGKEEMGKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVS 300
            G EEM KKEY  RT R+SRGMIE LKK   GENGGF CSSFDVIAAHLWKARTKA+SVS
Sbjct: 241 MGNEEMEKKEYGCRTLRVSRGMIETLKKKGFGENGGFPCSSFDVIAAHLWKARTKALSVS 300

Query: 301 NEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSR 360
            EKMVCLQFAV+ARNKL PPLPKGFSGN+FVLASV L A+QLE+ESYKTI+EMIR+AKS+
Sbjct: 301 KEKMVCLQFAVDARNKLKPPLPKGFSGNSFVLASVTLPAKQLEEESYKTIIEMIRDAKSQ 360

Query: 361 VDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDV 420
           VD+DY+NAY+ GL+GPQASLPPLKELTV+SDWTRMPFHKIEFLHG+A YASPLKPPILDV
Sbjct: 361 VDDDYVNAYQLGLDGPQASLPPLKELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 420

Query: 421 AYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 454
           AYFLQ+P DSQAIDVRV LL  TL+AFS+YFLT MQ
Sbjct: 421 AYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFLTSMQ 456

BLAST of CmaCh01G016510 vs. TAIR 10
Match: AT4G31910.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 425.2 bits (1092), Expect = 6.4e-119
Identity = 230/469 (49.04%), Postives = 318/469 (67.80%), Query Frame = 0

Query: 1   MATSHVSVTKTLTVFPKTLN--SPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTN 60
           +  + + + + L V+P+  N    KL+ LSNLDRQCPL +Y +FFYK +++   DS+F+N
Sbjct: 4   LMATRIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNTTTRDFDSVFSN 63

Query: 61  LKIGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSF-TNIEISELGDLSNYNE 120
           LK+GLEET+SVW PAAGRL  +    KLN+RCN+ GA++V++  T +++SELGDL+ YNE
Sbjct: 64  LKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQYNE 123

Query: 121 FFEKLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKS 180
           F+E LVYKP L+G+FS  PLVVAQVTRF CGGYSIG G SHSLFDG +AYEF+ AWA+ S
Sbjct: 124 FYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWASNS 183

Query: 181 AI-------FKDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLY 240
            I         +KKE   ++   PVH+R  LL++ DA R           + A AI HLY
Sbjct: 184 HIHNKSNSKITNKKEDVVIK---PVHDRRNLLVNRDAVRE----------TNAAAICHLY 243

Query: 241 RLIMQSVTGKEEMGK------KEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAA 300
           +LI Q++   +E  +        +V +TF L+   IE +KK ++    GF CSSF+ +AA
Sbjct: 244 QLIKQAMMTYQEQNRNLELPDSGFVIKTFELNGDAIESMKKKSL---EGFMCSSFEFLAA 303

Query: 301 HLWKARTKAMSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQ-LEDES 360
           HLWKART+A+ +  + MVCLQFAV+ R +   PLP+GFSGNA+VLASVA TAR+ LE+ +
Sbjct: 304 HLWKARTRALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELT 363

Query: 361 YKTIVEMIRNAKSRVDNDYINAYKQGLEGP-QASLPPLKELTVVSDWTRMPFHKIEFLHG 420
            ++IV  IR AK  +D  YIN+Y + L G    +LPPLKELT++SDWT+MPFH + F +G
Sbjct: 364 LESIVNKIREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNG 423

Query: 421 --QALYASPLKPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFL 450
              A Y +PL PP+  VAYF+++P D++ + VR+ L    +  FS++FL
Sbjct: 424 GEPADYMAPLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHFL 456

BLAST of CmaCh01G016510 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-45
Identity = 128/441 (29.02%), Postives = 215/441 (48.75%), Query Frame = 0

Query: 14  VFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLKIGLEETLSVWSPA 73
           V P++     L  LSNLD+   + V  I+ +K     + +++   +K  L + L  + P 
Sbjct: 41  VKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQV-IKKALSQVLVHYYPL 100

Query: 74  AGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYN-EFFEKLVYKPVLNGNF 133
           AGRL  +P  GKL + C   G + V++  N ++ E+GD++  + E   KLVY  V   N 
Sbjct: 101 AGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDAKNI 160

Query: 134 SEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFKDKKEPYGVELH 193
            E P V AQVT+F CGG+ +G  ++H +FDG  A EF+++W   +          G+ L 
Sbjct: 161 LEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVAR---------GLPLT 220

Query: 194 TPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKEEMGKKEYVYRT 253
           TP      +L   +  +          +     I+ LY              K+  +YR+
Sbjct: 221 TPPFSDRTILNARNPPKIENLHQEFEEIEDKSNINSLYT-------------KEPTLYRS 280

Query: 254 FRLSRGMIERLKKNAMGENG----GFACSSFDVIAAHLWKARTKAMSVSNEKMVCLQFAV 313
           F      I++LK  A  EN     G +C+SF+ ++A +W+ARTK++ + +++   L FAV
Sbjct: 281 FCFDPEKIKKLKLQAT-ENSESLLGNSCTSFEALSAFVWRARTKSLKMLSDQKTKLLFAV 340

Query: 314 NARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDNDYINAYKQ 373
           + R K  P LPKG+ GN  VL +    A +L ++     V ++R A   V + Y+ +   
Sbjct: 341 DGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAID 400

Query: 374 GLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYFLQSPLDSQ 433
             E  +A  P L    +++ W+R+ FH  +F  G+ + + P+  P  +V  FL      +
Sbjct: 401 YFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRR 455

Query: 434 AIDVRVPLLEGTLEAFSSYFL 450
           +I+V + L    ++ F   FL
Sbjct: 461 SINVLLGLPATAMDVFQEQFL 455

BLAST of CmaCh01G016510 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-45
Identity = 128/441 (29.02%), Postives = 215/441 (48.75%), Query Frame = 0

Query: 14  VFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLKIGLEETLSVWSPA 73
           V P++     L  LSNLD+   + V  I+ +K     + +++   +K  L + L  + P 
Sbjct: 25  VKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQV-IKKALSQVLVHYYPL 84

Query: 74  AGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYN-EFFEKLVYKPVLNGNF 133
           AGRL  +P  GKL + C   G + V++  N ++ E+GD++  + E   KLVY  V   N 
Sbjct: 85  AGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDAKNI 144

Query: 134 SEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFKDKKEPYGVELH 193
            E P V AQVT+F CGG+ +G  ++H +FDG  A EF+++W   +          G+ L 
Sbjct: 145 LEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVAR---------GLPLT 204

Query: 194 TPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKEEMGKKEYVYRT 253
           TP      +L   +  +          +     I+ LY              K+  +YR+
Sbjct: 205 TPPFSDRTILNARNPPKIENLHQEFEEIEDKSNINSLYT-------------KEPTLYRS 264

Query: 254 FRLSRGMIERLKKNAMGENG----GFACSSFDVIAAHLWKARTKAMSVSNEKMVCLQFAV 313
           F      I++LK  A  EN     G +C+SF+ ++A +W+ARTK++ + +++   L FAV
Sbjct: 265 FCFDPEKIKKLKLQAT-ENSESLLGNSCTSFEALSAFVWRARTKSLKMLSDQKTKLLFAV 324

Query: 314 NARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDNDYINAYKQ 373
           + R K  P LPKG+ GN  VL +    A +L ++     V ++R A   V + Y+ +   
Sbjct: 325 DGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAID 384

Query: 374 GLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYFLQSPLDSQ 433
             E  +A  P L    +++ W+R+ FH  +F  G+ + + P+  P  +V  FL      +
Sbjct: 385 YFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRR 439

Query: 434 AIDVRVPLLEGTLEAFSSYFL 450
           +I+V + L    ++ F   FL
Sbjct: 445 SINVLLGLPATAMDVFQEQFL 439

BLAST of CmaCh01G016510 vs. TAIR 10
Match: AT3G48720.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 179.9 bits (455), Expect = 4.7e-45
Identity = 120/420 (28.57%), Postives = 209/420 (49.76%), Query Frame = 0

Query: 27  LSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLKIGLEETLSVWSPAAGRLEPNPANGKL 86
           LSNLD+   + V  +++YK  S ++ +S +  +K  L E L  + P AGRL  +P  GK+
Sbjct: 30  LSNLDQNIAIIVKTLYYYKSESRTNQES-YNVIKKSLSEVLVHYYPVAGRLTISP-EGKI 89

Query: 87  NLRCNNRGAILVQSFTNIEISELGDLSNYN--EFFEKLVYKPVLNGNFSEFPLVVAQVTR 146
            + C   G ++V++  N  I  + +  + N  E  EKLVY      N  E P VV QVT 
Sbjct: 90  AVNCTGEGVVVVEAEANCGIDTIKEAISENRMETLEKLVYDVPGARNILEIPPVVVQVTN 149

Query: 147 FGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFKDKKEPYGVELHTPVHERGRLLMD 206
           F CGG+ +G G+SH++FDG AA EFL++W   +          G+ L  P        +D
Sbjct: 150 FKCGGFVLGLGMSHNMFDGVAAAEFLNSWCEMAK---------GLPLSVPP------FLD 209

Query: 207 YDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKEEMGKKEYVYRTFRLSRGMIERLK 266
               RS     +    +    I+ +      S TGK    +++ +Y++F      +E+LK
Sbjct: 210 RTILRSRNPPKIEFPHNEFDEIEDI------SDTGK-IYDEEKLIYKSFLFEPEKLEKLK 269

Query: 267 KNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEKMVCLQFAVNARNKLMPPLPKGFSG 326
             A+ EN     S+F  +   LWK+R +A+    ++ V L FA + R++ +P LP+G+ G
Sbjct: 270 IMAIEENNNNKVSTFQALTGFLWKSRCEALRFKPDQRVKLLFAADGRSRFIPRLPQGYCG 329

Query: 327 NAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDNDYINAYKQGLEGPQASLPPLKELT 386
           N  VL  +  ++ +L        V +++     V + ++ +     E    + P +    
Sbjct: 330 NGIVLTGLVTSSGELVGNPLSHSVGLVKRLVELVTDGFMRSAMDYFE-VNRTRPSMNATL 389

Query: 387 VVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYFLQSPLDSQAIDVRVPLLEGTLEAF 445
           +++ W+++  HK++F  G+ +++ P+  P  +V  FL S  D ++I+V + L    +E F
Sbjct: 390 LITSWSKLTLHKLDFGWGEPVFSGPVGLPGREVILFLPSGDDMKSINVFLGLPTSAMEVF 424

BLAST of CmaCh01G016510 vs. TAIR 10
Match: AT5G63560.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 166.0 bits (419), Expect = 7.0e-41
Identity = 125/443 (28.22%), Postives = 216/443 (48.76%), Query Frame = 0

Query: 6   VSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPSSSSSLDSIFTNLKIGLEE 65
           V+  + + V P +     L  LSNLD+   + V   +++K +S S+ +S +  +K  L E
Sbjct: 9   VTRKEPVLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNSRSNEES-YEVIKKSLSE 68

Query: 66  TLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDL---SNYNEFFEKL 125
            L  + PAAGRL  +P  GK+ + C   G ++V++  N  I ++       +  E  EKL
Sbjct: 69  VLVHYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETLEKL 128

Query: 126 VYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIFKD 185
           VY      N  E P VV QVT F CGG+ +G G++H++FDG AA EFL++WA  +     
Sbjct: 129 VYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETAR---- 188

Query: 186 KKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGKEE 245
                G+ L  P      LL      R+  K   P          H     ++ ++G  +
Sbjct: 189 -----GLPLSVPPFLDRTLL----RPRTPPKIEFP----------HNEFEDLEDISGTGK 248

Query: 246 M-GKKEYVYRTFRLSRGMIERLKKNAMGENGGFACSSFDVIAAHLWKARTKAMSVSNEKM 305
           +   ++ VY++F      +ERLK   M E      ++F  +   LW+AR +A+ +  ++ 
Sbjct: 249 LYSDEKLVYKSFLFGPEKLERLK--IMAET---RSTTFQTLTGFLWRARCQALGLKPDQR 308

Query: 306 VCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRNAKSRVDND 365
           + L FA + R++ +P LPKG+SGN  V      TA ++        V +++ A   V++ 
Sbjct: 309 IKLLFAADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDG 368

Query: 366 YINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPILDVAYFL 425
           ++ +     E  +A  P L    +++ W ++ FH  +F  G+ + + P+  P  +V  FL
Sbjct: 369 FMRSAIDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFL 420

Query: 426 QSPLDSQAIDVRVPLLEGTLEAF 445
               D+++I+V + L    ++ F
Sbjct: 429 PCGSDTKSINVLLGLPGSAMKVF 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ589.0e-11849.04Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT... [more]
Q94CD11.7e-4429.02Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FFQ79.8e-4028.22Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FA... [more]
A0PDV53.1e-3328.04Rosmarinate synthase OS=Plectranthus scutellarioides OX=4142 GN=RAS PE=1 SV=1[more]
Q8S9G69.8e-3226.57Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=2... [more]
Match NameE-valueIdentityDescription
A0A6J1IT612.4e-259100.00brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FK904.2e-25196.91brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1F9T52.6e-20880.66brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IG594.1e-20680.26brassinosteroid-related acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A1S3BW291.1e-19878.17omega-hydroxypalmitate O-feruloyl transferase OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
XP_022981142.15.0e-259100.00brassinosteroid-related acyltransferase 1 [Cucurbita maxima][more]
XP_022941116.18.6e-25196.91brassinosteroid-related acyltransferase 1-like [Cucurbita moschata][more]
KAG6608286.11.9e-25096.69Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp... [more]
XP_023524200.15.6e-25096.69brassinosteroid-related acyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6591543.12.8e-20980.92Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
AT4G31910.16.4e-11949.04HXXXD-type acyl-transferase family protein [more]
AT5G41040.11.2e-4529.02HXXXD-type acyl-transferase family protein [more]
AT5G41040.21.2e-4529.02HXXXD-type acyl-transferase family protein [more]
AT3G48720.14.7e-4528.57HXXXD-type acyl-transferase family protein [more]
AT5G63560.17.0e-4128.22HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..212
e-value: 1.4E-39
score: 137.8
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 233..449
e-value: 1.6E-48
score: 166.8
IPR003480TransferasePFAMPF02458Transferasecoord: 5..446
e-value: 5.7E-69
score: 232.8
NoneNo IPR availablePANTHERPTHR31642TRICHOTHECENE 3-O-ACETYLTRANSFERASEcoord: 5..450
NoneNo IPR availablePANTHERPTHR31642:SF145BRASSINOSTEROID-RELATED ACYLTRANSFERASE 1coord: 5..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G016510.1CmaCh01G016510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups