CmaCh01G016400 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G016400
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPectinesterase
LocationCma_Chr01: 11242300 .. 11244870 (-)
RNA-Seq ExpressionCmaCh01G016400
SyntenyCmaCh01G016400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTAATAGCTTCATTTTTTGTACTTTTAACTCTTATCAGTTCTTCTTTTGCTGCAGATGGGTCTCTCAATGATTTATTCCTTGCAAAAGCCATTTGCAAATTCACCTCAAATCCTTCTTACTGCATCTCTGTGCTTCAAAAATCCTCAGGCAATGTCTATGAATCAGGTAGGTTTTCGATGCGTCGGTCTTTGGCTAAAGCAACTACATTCTTGAACTTGATTGAGAAGCATCTTCAAAACAGTTCCACTCTACCAAAATCCGTCACTGGTGGTCTTAAAGATTGCCAATATCTTGCCCAACTAAACATTAATTTCTTGTCGAACTCTTTTCGGGCTGTCAATGGCACTGATAAAAAGCTAACCTACTCAAAAGCTGATTATATCCAGAGTCTTCTGAGTGCTATCTTAACCAACATTGATACTTGTCTTGATGGCCTAAATACCGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTCTTAGCACCACTCATTGATTGTACCAAATCATACAGTCTTTCTCTAGATCTTTTTACAAAGGGCTGGGTGCCTAGGAATAGAAACAGAACACGTGATCATCCTGTGACAAAACACCTCCGGTTTAGGAAAGGTCCGTTGCCATTGAGAATGTCAAAACATGACCGTGCTGTTTATAATTCGGTGGCGAATCGAAGAAGGCTTTCATCTTCTTCAGATGATGGAATTTTGGTTAATGGTATTGTAGTTGTGAGTCAAGATGGTACAGGAAACTTCCTCACCATCACAGAAGCTGTTAATGCAGCTCCAAACAACTCTCTTGCTGTCAATGGCTACTTCTTGATTTACATCGCAGCTGGTGTTTACCAAGAGTATGTGTCGATCCCTTCAAAGAAGAAGTACTTGCTCATGATTGGAGATGGTATAAATCAGACCATCATTACGGGCAACCAAAGCGTCGCTGACGGCTGGACTACCTTCAACAGTGCAACTTTTGGTAAGCATTTTGAAATACTATGTGATTAGAAACAAAAGCATGCTTTTGATTAACATTGATTGTCTTATACGAAATGATTTAAGGTTCAATTCTGAGAATACGTAAGATCTCATATTGGTTGGAGAGGAGAACGAAGCATTCCTTGTAAAAATGTGGAAACCTTCCCTAGTAGACACGTTTTAAAATTTTGAGGGGAAGCCCATAAGGAAAAGTTCAAAGAAGACAATGTCTACTAACAGTGGATTTGAGTTTTTACAAATGGTATCAGAGCCAGACACAAGCGGTGTGCCAGTGAGGACGCTAGGCTCCCAAAGGAGTAGATTGTGAGATCCCACGTTGGTTGGAGAAGAGAACGAAACATTCCTTATAAGGGGTGTTGATACCTCTCCCCAATAGATGCATTTTAAAACTTGGAAAGAAAGCTCAAAAGGGAAAGTCCAAAAAAGACCATATCTATGCTGTTTTAAAACTTTGAAGGGAAGCTTGGAAAAGCCCAAAAAAATAATATTTGTTAGCAGTGGATACTAATAAAAAGTGAATGGCATCATTAATTCAAGTTGATTTATTAGATGTGGCTGGAATTTCAAACTTCAAATATGTAATCATCTAAACTAAGCCAAAGTTTAAGATGTCATTTGGTTTTACATGAATGATGCAGCTGTGGCGGCAGAAGGATTCATGGCAGTTAACATAACCTTCCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCAATGAGAAGCGGGGCCGATATGTCCATCTTCTACAGCTGCAGCTTTGAGGGTTACCAGGACACCTTATACACTCACTCCCTAAGACAATTCTTCAGAGAATGTGACATTTATGGCACAGTGGACTTCATATTTGGAAATGCAGCTGTTGTGTTCCAAAACTGCAACATATATCCCCGTCTACCACGTTCTGGACAGACCAACATGATCACTGCTCAAGGTCGATCGGACCCGAACCAAAACACCGGTACTTCGATCCACAACTGCACAATCCGAGCCACGAGCGATTTAGCTGCAAGCAAGAGTTACATGAACAAGACATATTTGGGAAGGCCATGGAAGCAATATTCAAGAACAGTTTATATGCAGACATTCATTGATGGGTTCGTAAATCCTGCTGGTTGGGATCCATGGTCAGGGGAATACTTGAGCACATTGTATTATGCAGAGTATAATAATACAGGAGCTGGATCAAATACAAAGAACAGAGTCACCTGGCCTGGTTACCATGTCATTTCTAATGCTACTGATGCTTCTAATTTTACAGTTTCAAACTTCCTGGTTGGGGATGCGTGGCTGCCTCCAACTGGTGTGCCCTACACTGGAGGCTTCATATAACCGGTCAGCTCTCCATATAATTCTTGCTAATATTCAACATGCCTAATAAAGTTGTTTGTGCTCTGTTTTGAACTAATGATAGTTCTTACACTAGCAAGTTAATTGTATGATTAAGGCCAAATGAATATTTAGTCTTTTTTTTATGTCTTAATTTGGTCTCGGTCGTTAATTTTAGTTTAATCCAGTTTCTAATGTTCTCAAATTATTTAAATATAAATGAGTTAAAATAGGTTTGATTCTCA

mRNA sequence

ATGGCTTTAATAGCTTCATTTTTTGTACTTTTAACTCTTATCAGTTCTTCTTTTGCTGCAGATGGGTCTCTCAATGATTTATTCCTTGCAAAAGCCATTTGCAAATTCACCTCAAATCCTTCTTACTGCATCTCTGTGCTTCAAAAATCCTCAGGCAATGTCTATGAATCAGGTAGGTTTTCGATGCGTCGGTCTTTGGCTAAAGCAACTACATTCTTGAACTTGATTGAGAAGCATCTTCAAAACAGTTCCACTCTACCAAAATCCGTCACTGGTGGTCTTAAAGATTGCCAATATCTTGCCCAACTAAACATTAATTTCTTGTCGAACTCTTTTCGGGCTGTCAATGGCACTGATAAAAAGCTAACCTACTCAAAAGCTGATTATATCCAGAGTCTTCTGAGTGCTATCTTAACCAACATTGATACTTGTCTTGATGGCCTAAATACCGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTCTTAGCACCACTCATTGATTGTACCAAATCATACAGTCTTTCTCTAGATCTTTTTACAAAGGGCTGGGTGCCTAGGAATAGAAACAGAACACGTGATCATCCTGTGACAAAACACCTCCGGTTTAGGAAAGGTCCGTTGCCATTGAGAATGTCAAAACATGACCGTGCTGTTTATAATTCGGTGGCGAATCGAAGAAGGCTTTCATCTTCTTCAGATGATGGAATTTTGGTTAATGGTATTGTAGTTGTGAGTCAAGATGGTACAGGAAACTTCCTCACCATCACAGAAGCTGTTAATGCAGCTCCAAACAACTCTCTTGCTGTCAATGGCTACTTCTTGATTTACATCGCAGCTGGTGTTTACCAAGAGTATGTGTCGATCCCTTCAAAGAAGAAGTACTTGCTCATGATTGGAGATGGTATAAATCAGACCATCATTACGGGCAACCAAAGCGTCGCTGACGGCTGGACTACCTTCAACAGTGCAACTTTTGCTGTGGCGGCAGAAGGATTCATGGCAGTTAACATAACCTTCCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCAATGAGAAGCGGGGCCGATATGTCCATCTTCTACAGCTGCAGCTTTGAGGGTTACCAGGACACCTTATACACTCACTCCCTAAGACAATTCTTCAGAGAATGTGACATTTATGGCACAGTGGACTTCATATTTGGAAATGCAGCTGTTGTGTTCCAAAACTGCAACATATATCCCCGTCTACCACGTTCTGGACAGACCAACATGATCACTGCTCAAGGTCGATCGGACCCGAACCAAAACACCGGTACTTCGATCCACAACTGCACAATCCGAGCCACGAGCGATTTAGCTGCAAGCAAGAGTTACATGAACAAGACATATTTGGGAAGGCCATGGAAGCAATATTCAAGAACAGTTTATATGCAGACATTCATTGATGGGTTCGTAAATCCTGCTGGTTGGGATCCATGGTCAGGGGAATACTTGAGCACATTGTATTATGCAGAGTATAATAATACAGGAGCTGGATCAAATACAAAGAACAGAGTCACCTGGCCTGGTTACCATGTCATTTCTAATGCTACTGATGCTTCTAATTTTACAGTTTCAAACTTCCTGGTTGGGGATGCGTGGCTGCCTCCAACTGGTGTGCCCTACACTGGAGGCTTCATATAACCGGTCAGCTCTCCATATAATTCTTGCTAATATTCAACATGCCTAATAAAGTTGTTTGTGCTCTGTTTTGAACTAATGATAGTTCTTACACTAGCAAGTTAATTGTATGATTAAGGCCAAATGAATATTTAGTCTTTTTTTTATGTCTTAATTTGGTCTCGGTCGTTAATTTTAGTTTAATCCAGTTTCTAATGTTCTCAAATTATTTAAATATAAATGAGTTAAAATAGGTTTGATTCTCA

Coding sequence (CDS)

ATGGCTTTAATAGCTTCATTTTTTGTACTTTTAACTCTTATCAGTTCTTCTTTTGCTGCAGATGGGTCTCTCAATGATTTATTCCTTGCAAAAGCCATTTGCAAATTCACCTCAAATCCTTCTTACTGCATCTCTGTGCTTCAAAAATCCTCAGGCAATGTCTATGAATCAGGTAGGTTTTCGATGCGTCGGTCTTTGGCTAAAGCAACTACATTCTTGAACTTGATTGAGAAGCATCTTCAAAACAGTTCCACTCTACCAAAATCCGTCACTGGTGGTCTTAAAGATTGCCAATATCTTGCCCAACTAAACATTAATTTCTTGTCGAACTCTTTTCGGGCTGTCAATGGCACTGATAAAAAGCTAACCTACTCAAAAGCTGATTATATCCAGAGTCTTCTGAGTGCTATCTTAACCAACATTGATACTTGTCTTGATGGCCTAAATACCGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTCTTAGCACCACTCATTGATTGTACCAAATCATACAGTCTTTCTCTAGATCTTTTTACAAAGGGCTGGGTGCCTAGGAATAGAAACAGAACACGTGATCATCCTGTGACAAAACACCTCCGGTTTAGGAAAGGTCCGTTGCCATTGAGAATGTCAAAACATGACCGTGCTGTTTATAATTCGGTGGCGAATCGAAGAAGGCTTTCATCTTCTTCAGATGATGGAATTTTGGTTAATGGTATTGTAGTTGTGAGTCAAGATGGTACAGGAAACTTCCTCACCATCACAGAAGCTGTTAATGCAGCTCCAAACAACTCTCTTGCTGTCAATGGCTACTTCTTGATTTACATCGCAGCTGGTGTTTACCAAGAGTATGTGTCGATCCCTTCAAAGAAGAAGTACTTGCTCATGATTGGAGATGGTATAAATCAGACCATCATTACGGGCAACCAAAGCGTCGCTGACGGCTGGACTACCTTCAACAGTGCAACTTTTGCTGTGGCGGCAGAAGGATTCATGGCAGTTAACATAACCTTCCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCAATGAGAAGCGGGGCCGATATGTCCATCTTCTACAGCTGCAGCTTTGAGGGTTACCAGGACACCTTATACACTCACTCCCTAAGACAATTCTTCAGAGAATGTGACATTTATGGCACAGTGGACTTCATATTTGGAAATGCAGCTGTTGTGTTCCAAAACTGCAACATATATCCCCGTCTACCACGTTCTGGACAGACCAACATGATCACTGCTCAAGGTCGATCGGACCCGAACCAAAACACCGGTACTTCGATCCACAACTGCACAATCCGAGCCACGAGCGATTTAGCTGCAAGCAAGAGTTACATGAACAAGACATATTTGGGAAGGCCATGGAAGCAATATTCAAGAACAGTTTATATGCAGACATTCATTGATGGGTTCGTAAATCCTGCTGGTTGGGATCCATGGTCAGGGGAATACTTGAGCACATTGTATTATGCAGAGTATAATAATACAGGAGCTGGATCAAATACAAAGAACAGAGTCACCTGGCCTGGTTACCATGTCATTTCTAATGCTACTGATGCTTCTAATTTTACAGTTTCAAACTTCCTGGTTGGGGATGCGTGGCTGCCTCCAACTGGTGTGCCCTACACTGGAGGCTTCATATAA

Protein sequence

MALIASFFVLLTLISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFLNLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI
Homology
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BLAST of CmaCh01G016400 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 636.0 bits (1639), Expect = 4.1e-181
Identity = 319/533 (59.85%), Postives = 399/533 (74.86%), Query Frame = 0 

Query: 33  ICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFLNLIEKHL-QNSSTLPKSVT 92
           IC+F  +PSYC SVL    G++Y  GR S+RRSL++A  F+++I+  L +      KS  
Sbjct: 38  ICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKSTV 97

Query: 93  GGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLNTV 152
           G L+DC++LA L +++L +S +  + T K L+ S+A+ + + LSA +TN  TCL+GL + 
Sbjct: 98  GALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKST 157

Query: 153 ASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPV-------TKHLRFRK 212
           AS + L  D    L + TK Y +SL LF+KGWVPR   R R  P+        K   FR 
Sbjct: 158 ASENGLSGD----LFNDTKLYGVSLALFSKGWVPR---RQRSRPIWQPQARFKKFFGFRN 217

Query: 213 GPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVNAAPN 272
           G LPL+M++  RAVYN+V  RR+L  S  D + V+ IV V Q+GTGNF TI  A+ AAPN
Sbjct: 218 GKLPLKMTERARAVYNTV-TRRKLLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPN 277

Query: 273 NSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSAT 332
            +   NGYFLIY+ AG+Y+EYV +P  K+Y++MIGDGINQT+ITGN+SV DGWTTFNSAT
Sbjct: 278 KTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSAT 337

Query: 333 FAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFR 392
           F ++   F+ VNIT +NTAG  KGQAVA+RSG D+S+FYSCSFE YQDTLYTHSLRQF+R
Sbjct: 338 FILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYR 397

Query: 393 ECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRA 452
           ECD+YGTVDFIFGNAAVV QNCN+YPR PR GQ+N +TAQGR+DPNQNTGT+IH CTIR 
Sbjct: 398 ECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRP 457

Query: 453 TSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY-LSTLYYAEYN 512
             DLA S +Y  KTYLGRPWK+YSRTV MQT+IDGF+ P+GW+ WSG++ LSTLYYAEYN
Sbjct: 458 ADDLATS-NYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYN 517

Query: 513 NTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 557
           NTG GS+T NRVTWPGYHVI NATDASNFTV+NFLVG+ W+  TGVP+ GG I
Sbjct: 518 NTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

[Close]
BLAST of CmaCh01G016400 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 627.9 bits (1618), Expect = 1.1e-178
Identity = 330/574 (57.49%), Postives = 419/574 (73.00%), Query Frame = 0 

Query: 1   MALIASFFVLLTL-ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVL-QKSSGNVYESG 60
           M  +  F V L L + + F A  +L     +  ICK T +P +C SV  Q S G+V E G
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYG 60

Query: 61  RFSMRRSLAKATTFLNLIEKHL-QNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNG 120
           RFS+R+SL ++  F   I+++L +N++ L +S  G L+DC+YLA L  ++L  SF  VN 
Sbjct: 61  RFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNI 120

Query: 121 T--DKKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSL 180
           T   K L++SKAD IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+
Sbjct: 121 TTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSV 180

Query: 181 SLDLFTKGWVPRNRNRTRD----HPVTKH------LRFRKGPLPLRMSKHDRAVYNSVAN 240
           SL LFTKGWVP+ + +       HP   H        FR G LPL+M++H RAVY S++ 
Sbjct: 181 SLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSR 240

Query: 241 RRRLSSSSD-DGILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVYQ 300
           R+     +D + +LV+ IV V+Q+GTGNF TITEAVN+APN +    GYF+IY+ +GVY+
Sbjct: 241 RKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYE 300

Query: 301 EYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNTA 360
           E V I   K+YL+MIGDGIN+T++TGN++V DGWTTFNSATFAV +  F+AVN+TF+NTA
Sbjct: 301 ENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTA 360

Query: 361 GAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVF 420
           G  K QAVAMRS AD+SIFYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVF
Sbjct: 361 GPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVF 420

Query: 421 QNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGRP 480
           Q+CN+YPR P   Q N ITAQGR+DPNQNTG SIHNCTI+   DL +S +Y  KTYLGRP
Sbjct: 421 QDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKTYLGRP 480

Query: 481 WKQYSRTVYMQTFIDGFVNPAGWDPWSGEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHV 540
           WK+YSRTV+MQ++ID  V P GW  W+G++ LSTLYYAEYNNTG+GS+T +RV WPGYHV
Sbjct: 481 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 540

Query: 541 ISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 557
           I N+TDA+NFTV NFL+GD W+  +GVPY  G +
Sbjct: 541 I-NSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

[Close]
BLAST of CmaCh01G016400 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 8.0e-177
Identity = 329/575 (57.22%), Postives = 412/575 (71.65%), Query Frame = 0 

Query: 8   FVLLTLISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLA 67
           F+L+TL     +   S   L  +  ICK T +P YC SV   S GNV + G FS+R+SL+
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 68  KATTFLNLIEKHLQNSSTLPK-SVTGGLKDCQYLAQLNINFLSNSFRAVNGTD-----KK 127
           ++  F+  ++++++ ++ L + +V   L+DC++LA L +++L  SF  VN T      K 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 128 LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS---SLEKDLLAPLIDCTKSYSLSLDL 187
           L++ KAD IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL L
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 188 FTKGWVPRNRN---------RTRDHPVTKHLR-FRKGPLPLRMSKHDRAVYNSVANRRRL 247
           FTKGWVP+ +          R+     TK  R FR G LPL+M++  +AVY S++ R+  
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 248 SSSS----DDG--ILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVY 307
              S    DDG  +L++ IV VSQDGTGNF  IT AV AAPNN+    G+FLIY+ AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 308 QEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNT 367
           +EY+SI   K+Y++MIGDGINQT++TGN+SV DGWTTFNSATFAV A  F+AVNITF+NT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 368 AGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVV 427
           AG  K QAVA+RSGAD SIFYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 428 FQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGR 487
           FQNCN+YPR P   Q N ITAQGRSDPNQNTGTSI NCTI+   DL +S +Y  KTYLGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSS-NYTVKTYLGR 485

Query: 488 PWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYH 547
           PWK+YSRTVYMQ++IDGFV P GW  W+G++ LSTLYYAEYNNTG GSNT NRVTWPGYH
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 548 VISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 557
           VI N+TDA+NFTV+   +   W+  TGVPYT G I
Sbjct: 546 VI-NSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

[Close]
BLAST of CmaCh01G016400 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 6.9e-128
Identity = 252/545 (46.24%), Postives = 345/545 (63.30%), Query Frame = 0 

Query: 34  CKFTSNPSYCISVL---QKSSGNVYESGRFSMRRSLAKATTFLNLIEKHLQNSSTLPKSV 93
           CK T  P  C ++L   + S  + Y  G+F++++ L +A+    +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 94  T----GGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLD 153
           T    G + DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 154 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKGWVPRNRNRTRD 213
           GL  V + S     + +P+ + T+ YS+SL L              +KG +    N T  
Sbjct: 203 GL--VEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGGGNSTYR 262

Query: 214 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 273
            P+   ++  +     +   +D+    +  N   L  +S   ILV+  V+V    + NF 
Sbjct: 263 EPLETLIKGLR-----KTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFT 322

Query: 274 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 333
           TIT+A+ AAPNN+   +GYF+IY   GVY+EY+ +P  KK L+++GDGIN+TIITGN +V
Sbjct: 323 TITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNV 382

Query: 334 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 393
            DGWTT+N ++FAV  E FMAV++TF+NTAG  K QAVA+R+ A+ S FY CSFEGYQDT
Sbjct: 383 VDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDT 442

Query: 394 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 453
           LY HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P + Q N ITA GR DPNQNT
Sbjct: 443 LYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNT 502

Query: 454 GTSIHNCTIRATSDLAAS-KSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGE 513
           G SI NCTI+A  DLAA  KS M  T+LGRPWK YSRTV+MQ++I   V P GW  W+G 
Sbjct: 503 GISIINCTIKAAPDLAAEPKSAM--TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGT 562

Query: 514 Y-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPY 557
             L T+YY EY+N G G+NT  RV W GY+++ N  +A NFTV NF +GD WLP T +P+
Sbjct: 563 IGLDTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQTDIPF 616

[Close]
BLAST of CmaCh01G016400 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 5.1e-123
Identity = 256/544 (47.06%), Postives = 333/544 (61.21%), Query Frame = 0 

Query: 34  CKFTSNPSYC---ISVLQKSSGNVYESGRFSMRRSLAKATTFLNLIEKHLQNSSTLPKSV 93
           CK T  P  C   +S ++ S  + Y  G+F+M++ L +A     +I +  Q     P + 
Sbjct: 88  CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEADPGTS 147

Query: 94  T----GGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLD 153
           T      + DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN  TCLD
Sbjct: 148 TVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQQTCLD 207

Query: 154 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHLRFRKGP 213
           GL    SG +    +  PL + T+ YS+SL L +      NRN  R +  +K   F  G 
Sbjct: 208 GLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA---LNRNLKR-YKGSKGKIFGGGN 267

Query: 214 LPLR----------MSKHDRAVYNSVANRR--RLSSSSDDGILVNGIVVVSQDGTGNFLT 273
            P+R              D+      ANR    L  +S   ILV   V V    T NF T
Sbjct: 268 KPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDNFPT 327

Query: 274 ITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVA 333
           ITEAV AAPN++    GYF+IY  AG+Y+EYV I +KK+ +++IGDGIN+TII+GN S  
Sbjct: 328 ITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSFI 387

Query: 334 DGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTL 393
           DGWTT+NS+TFAV  + F+AV++TF+NTAG  K QAVA+R+ AD S FY CSFEGYQDTL
Sbjct: 388 DGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTL 447

Query: 394 YTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTG 453
           Y HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P + Q N +TA GR+DPNQ TG
Sbjct: 448 YVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTG 507

Query: 454 TSIHNCTIRATSDLAAS-KSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 513
            SI NCTI A  DLAA  KS M  T+LGRPWK YSRTVY+Q++I   V P GW  W+G  
Sbjct: 508 ISIINCTIGAAPDLAADPKSTM--TFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTT 567

Query: 514 -LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYT 557
            L T+ Y EY+N G G++T  RV W GY ++ N   A NFTV NF +GD WLP T +P+ 
Sbjct: 568 GLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQTDIPFY 621

[Close]
BLAST of CmaCh01G016400 vs. ExPASy TrEMBL
Match: A0A6J1J323 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480894 PE=3 SV=1)

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 537/543 (98.90%), Postives = 538/543 (99.08%), Query Frame = 0 

Query: 14  ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 73
           +   F  DGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL
Sbjct: 1   MQGGFHPDGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 60

Query: 74  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 133
           NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL
Sbjct: 61  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 120

Query: 134 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 193
           LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD
Sbjct: 121 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 180

Query: 194 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 253
           HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL
Sbjct: 181 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 240

Query: 254 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 313
           TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV
Sbjct: 241 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 300

Query: 314 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 373
           ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT
Sbjct: 301 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 360

Query: 374 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 433
           LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT
Sbjct: 361 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 420

Query: 434 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 493
           GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY
Sbjct: 421 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 480

Query: 494 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 553
           LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG
Sbjct: 481 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 540

Query: 554 GFI 557
           GFI
Sbjct: 541 GFI 543

[Close]
BLAST of CmaCh01G016400 vs. ExPASy TrEMBL
Match: A0A6J1FJR9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111446371 PE=3 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 4.5e-308
Identity = 529/543 (97.42%), Postives = 535/543 (98.53%), Query Frame = 0 

Query: 14  ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 73
           +   F  DGSLNDLF AKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSL+KA+ FL
Sbjct: 1   MQGGFHPDGSLNDLFAAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKASAFL 60

Query: 74  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 133
           NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL
Sbjct: 61  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 120

Query: 134 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 193
           LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD
Sbjct: 121 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 180

Query: 194 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 253
           HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRL SSSDDGILVNGIVVVSQDGTGNFL
Sbjct: 181 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLLSSSDDGILVNGIVVVSQDGTGNFL 240

Query: 254 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 313
           TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV
Sbjct: 241 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 300

Query: 314 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 373
           ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT
Sbjct: 301 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 360

Query: 374 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 433
           LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT
Sbjct: 361 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 420

Query: 434 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 493
           GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGF+NPAGWDPWSGEY
Sbjct: 421 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEY 480

Query: 494 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 553
           LSTLYYAEYNN+GAGSNTKNRVTWPGYHVISNAT+ASNFTVSNFLVGDAWLPPTGVPYTG
Sbjct: 481 LSTLYYAEYNNSGAGSNTKNRVTWPGYHVISNATEASNFTVSNFLVGDAWLPPTGVPYTG 540

Query: 554 GFI 557
           GFI
Sbjct: 541 GFI 543

[Close]
BLAST of CmaCh01G016400 vs. ExPASy TrEMBL
Match: A0A5A7TAP2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005740 PE=3 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.3e-283
Identity = 483/552 (87.50%), Postives = 519/552 (94.02%), Query Frame = 0 

Query: 7   FFVLLTLISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKS-SGNVYESGRFSMRRS 66
           F +LLTL +SSFA+D SL ++F  KAICK TSNPSYCI+VL++S  GN+Y+SGRFS+RRS
Sbjct: 11  FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRS 70

Query: 67  LAKATTFLNLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYS 126
           L+KA+ FL+LIEKHLQNSSTLPKS+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+KLTYS
Sbjct: 71  LSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYS 130

Query: 127 KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 186
           KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP
Sbjct: 131 KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 190

Query: 187 R-NRNRTRDHPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVV 246
           R NRNRT +HP  KHL+FRKGPLPLRMS+HDRAVYNSVANRR+LSSSSDDG+LVNGI VV
Sbjct: 191 RWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVV 250

Query: 247 SQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQ 306
           SQDG G+FL IT+A+NAAPNNSLA NGYFLIYI AGVYQEYVS+PSKKKYLLMIGDGINQ
Sbjct: 251 SQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQ 310

Query: 307 TIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYS 366
           TIITGN+SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM +FYS
Sbjct: 311 TIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYS 370

Query: 367 CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQ 426
           CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP   Q NMITAQ
Sbjct: 371 CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQ 430

Query: 427 GRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPA 486
           GRSDPNQNTGTSIHNCTI+AT DLAAS S+MNKTYLGRPWKQYSRTVYMQTFIDGFVNP 
Sbjct: 431 GRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPK 490

Query: 487 GWDPWSGEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWL 546
           GWDPWSGEYLSTLYY EYNNTG GS+TKNRV WPGYHVI+NATDA+NFT+SNFLVGD WL
Sbjct: 491 GWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWL 550

Query: 547 PPTGVPYTGGFI 557
           PPTGVPYTGGF+
Sbjct: 551 PPTGVPYTGGFV 562

[Close]
BLAST of CmaCh01G016400 vs. ExPASy TrEMBL
Match: A0A1S4E6G9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.3e-283
Identity = 483/552 (87.50%), Postives = 519/552 (94.02%), Query Frame = 0 

Query: 7   FFVLLTLISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKS-SGNVYESGRFSMRRS 66
           F +LLTL +SSFA+D SL ++F  KAICK TSNPSYCI+VL++S  GN+Y+SGRFS+RRS
Sbjct: 11  FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRS 70

Query: 67  LAKATTFLNLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYS 126
           L+KA+ FL+LIEKHLQNSSTLPKS+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+KLTYS
Sbjct: 71  LSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYS 130

Query: 127 KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 186
           KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP
Sbjct: 131 KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 190

Query: 187 R-NRNRTRDHPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVV 246
           R NRNRT +HP  KHL+FRKGPLPLRMS+HDRAVYNSVANRR+LSSSSDDG+LVNGI VV
Sbjct: 191 RWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVV 250

Query: 247 SQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQ 306
           SQDG G+FL IT+A+NAAPNNSLA NGYFLIYI AGVYQEYVS+PSKKKYLLMIGDGINQ
Sbjct: 251 SQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQ 310

Query: 307 TIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYS 366
           TIITGN+SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM +FYS
Sbjct: 311 TIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYS 370

Query: 367 CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQ 426
           CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP   Q NMITAQ
Sbjct: 371 CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQ 430

Query: 427 GRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPA 486
           GRSDPNQNTGTSIHNCTI+AT DLAAS S+MNKTYLGRPWKQYSRTVYMQTFIDGFVNP 
Sbjct: 431 GRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPK 490

Query: 487 GWDPWSGEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWL 546
           GWDPWSGEYLSTLYY EYNNTG GS+TKNRV WPGYHVI+NATDA+NFT+SNFLVGD WL
Sbjct: 491 GWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWL 550

Query: 547 PPTGVPYTGGFI 557
           PPTGVPYTGGF+
Sbjct: 551 PPTGVPYTGGFV 562

[Close]
BLAST of CmaCh01G016400 vs. ExPASy TrEMBL
Match: A0A0A0LHM0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G890080 PE=3 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 8.6e-283
Identity = 483/556 (86.87%), Postives = 521/556 (93.71%), Query Frame = 0 

Query: 3   LIASFFVLLTLISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKS-SGNVYESGRFS 62
           L+ASF +LLTL +SSFA+D SL + F  KAICK TSNPSYCI+VL++S  GN+Y+SGRFS
Sbjct: 10  LLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFS 69

Query: 63  MRRSLAKATTFLNLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKK 122
           +RRSL+KAT FL+LIEKHLQNSSTLP S+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+K
Sbjct: 70  IRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRK 129

Query: 123 LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTK 182
           LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTK
Sbjct: 130 LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTK 189

Query: 183 GWVP-RNRNRTRDHPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNG 242
           GWVP RNRNRT +HP  KHL+FRKGPLPLRMS+HDRAVYNSVANRR+LSSSSDDG+LVNG
Sbjct: 190 GWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNG 249

Query: 243 IVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGD 302
           +VVVSQDG G+FL IT+A+NAAPNNSLA +GYFLIYI AGVYQEYVS+PSKKKYLLMIGD
Sbjct: 250 VVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGD 309

Query: 303 GINQTIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMS 362
           GINQTIITGN+SVADGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAVA+RSGADM 
Sbjct: 310 GINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMC 369

Query: 363 IFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNM 422
           +FYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPR GQ NM
Sbjct: 370 VFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANM 429

Query: 423 ITAQGRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGF 482
           ITAQGRSDPNQNTGTSIHNCTIRAT +LAAS SYMNKTYLGRPWKQYSRTVYMQTFIDGF
Sbjct: 430 ITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGF 489

Query: 483 VNPAGWDPWSGEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVG 542
           VNP GWDPW+GEYLSTLYY EYNNTG GS+TKNRVTW GYHVI+N TDA+NFT+SNFLVG
Sbjct: 490 VNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVG 549

Query: 543 DAWLPPTGVPYTGGFI 557
           DAWLPPT VPY GGF+
Sbjct: 550 DAWLPPTWVPYMGGFV 565

[Close]
BLAST of CmaCh01G016400 vs. NCBI nr
Match: XP_022981882.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita maxima] >XP_022981883.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita maxima] >XP_022981884.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 537/543 (98.90%), Postives = 538/543 (99.08%), Query Frame = 0 

Query: 14  ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 73
           +   F  DGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL
Sbjct: 1   MQGGFHPDGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 60

Query: 74  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 133
           NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL
Sbjct: 61  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 120

Query: 134 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 193
           LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD
Sbjct: 121 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 180

Query: 194 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 253
           HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL
Sbjct: 181 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 240

Query: 254 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 313
           TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV
Sbjct: 241 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 300

Query: 314 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 373
           ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT
Sbjct: 301 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 360

Query: 374 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 433
           LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT
Sbjct: 361 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 420

Query: 434 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 493
           GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY
Sbjct: 421 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 480

Query: 494 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 553
           LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG
Sbjct: 481 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 540

Query: 554 GFI 557
           GFI
Sbjct: 541 GFI 543

[Close]
BLAST of CmaCh01G016400 vs. NCBI nr
Match: KAG6608276.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.4e-309
Identity = 532/543 (97.97%), Postives = 536/543 (98.71%), Query Frame = 0 

Query: 14  ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 73
           +   F  DGSLN LF AKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSL+KATTFL
Sbjct: 1   MQGGFHPDGSLNGLFPAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKATTFL 60

Query: 74  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 133
           NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL
Sbjct: 61  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 120

Query: 134 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 193
           LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD
Sbjct: 121 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 180

Query: 194 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 253
           HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL
Sbjct: 181 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 240

Query: 254 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 313
           TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV
Sbjct: 241 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 300

Query: 314 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 373
           ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT
Sbjct: 301 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 360

Query: 374 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 433
           LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT
Sbjct: 361 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 420

Query: 434 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 493
           GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGF+NPAGWDPWSGEY
Sbjct: 421 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEY 480

Query: 494 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 553
           LSTLYYAEYNN+GAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG
Sbjct: 481 LSTLYYAEYNNSGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 540

Query: 554 GFI 557
           GFI
Sbjct: 541 GFI 543

[Close]
BLAST of CmaCh01G016400 vs. NCBI nr
Match: XP_023524144.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524145.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524146.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 531/543 (97.79%), Postives = 535/543 (98.53%), Query Frame = 0 

Query: 14  ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 73
           +   F  DGSLNDLF AKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSL+KA+ FL
Sbjct: 1   MQGGFHPDGSLNDLFPAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKASAFL 60

Query: 74  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 133
           NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL
Sbjct: 61  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 120

Query: 134 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 193
           LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD
Sbjct: 121 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 180

Query: 194 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 253
           HPVTKHLRFRKGPLPLRMSKHDR VYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL
Sbjct: 181 HPVTKHLRFRKGPLPLRMSKHDRVVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 240

Query: 254 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 313
           TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV
Sbjct: 241 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 300

Query: 314 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 373
           ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT
Sbjct: 301 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 360

Query: 374 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 433
           LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT
Sbjct: 361 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 420

Query: 434 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 493
           GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGF+NPAGWDPWSGEY
Sbjct: 421 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEY 480

Query: 494 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 553
           LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG
Sbjct: 481 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 540

Query: 554 GFI 557
           GFI
Sbjct: 541 GFI 543

[Close]
BLAST of CmaCh01G016400 vs. NCBI nr
Match: XP_022940931.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita moschata] >XP_022940932.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita moschata] >XP_022940933.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1066.2 bits (2756), Expect = 9.4e-308
Identity = 529/543 (97.42%), Postives = 535/543 (98.53%), Query Frame = 0 

Query: 14  ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFL 73
           +   F  DGSLNDLF AKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSL+KA+ FL
Sbjct: 1   MQGGFHPDGSLNDLFAAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKASAFL 60

Query: 74  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 133
           NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL
Sbjct: 61  NLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSL 120

Query: 134 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 193
           LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD
Sbjct: 121 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRD 180

Query: 194 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 253
           HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRL SSSDDGILVNGIVVVSQDGTGNFL
Sbjct: 181 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLLSSSDDGILVNGIVVVSQDGTGNFL 240

Query: 254 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 313
           TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV
Sbjct: 241 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 300

Query: 314 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 373
           ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT
Sbjct: 301 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 360

Query: 374 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 433
           LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT
Sbjct: 361 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 420

Query: 434 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 493
           GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGF+NPAGWDPWSGEY
Sbjct: 421 GTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEY 480

Query: 494 LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTG 553
           LSTLYYAEYNN+GAGSNTKNRVTWPGYHVISNAT+ASNFTVSNFLVGDAWLPPTGVPYTG
Sbjct: 481 LSTLYYAEYNNSGAGSNTKNRVTWPGYHVISNATEASNFTVSNFLVGDAWLPPTGVPYTG 540

Query: 554 GFI 557
           GFI
Sbjct: 541 GFI 543

[Close]
BLAST of CmaCh01G016400 vs. NCBI nr
Match: XP_016903565.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo] >KAA0038671.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa] >TYK31280.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 984.2 bits (2543), Expect = 4.7e-283
Identity = 483/552 (87.50%), Postives = 519/552 (94.02%), Query Frame = 0 

Query: 7   FFVLLTLISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKS-SGNVYESGRFSMRRS 66
           F +LLTL +SSFA+D SL ++F  KAICK TSNPSYCI+VL++S  GN+Y+SGRFS+RRS
Sbjct: 11  FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRS 70

Query: 67  LAKATTFLNLIEKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYS 126
           L+KA+ FL+LIEKHLQNSSTLPKS+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+KLTYS
Sbjct: 71  LSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYS 130

Query: 127 KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 186
           KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP
Sbjct: 131 KADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 190

Query: 187 R-NRNRTRDHPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVV 246
           R NRNRT +HP  KHL+FRKGPLPLRMS+HDRAVYNSVANRR+LSSSSDDG+LVNGI VV
Sbjct: 191 RWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVV 250

Query: 247 SQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQ 306
           SQDG G+FL IT+A+NAAPNNSLA NGYFLIYI AGVYQEYVS+PSKKKYLLMIGDGINQ
Sbjct: 251 SQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQ 310

Query: 307 TIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYS 366
           TIITGN+SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM +FYS
Sbjct: 311 TIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYS 370

Query: 367 CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQ 426
           CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP   Q NMITAQ
Sbjct: 371 CSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQ 430

Query: 427 GRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPA 486
           GRSDPNQNTGTSIHNCTI+AT DLAAS S+MNKTYLGRPWKQYSRTVYMQTFIDGFVNP 
Sbjct: 431 GRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPK 490

Query: 487 GWDPWSGEYLSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWL 546
           GWDPWSGEYLSTLYY EYNNTG GS+TKNRV WPGYHVI+NATDA+NFT+SNFLVGD WL
Sbjct: 491 GWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWL 550

Query: 547 PPTGVPYTGGFI 557
           PPTGVPYTGGF+
Sbjct: 551 PPTGVPYTGGFV 562

[Close]
BLAST of CmaCh01G016400 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 636.0 bits (1639), Expect = 2.9e-182
Identity = 319/533 (59.85%), Postives = 399/533 (74.86%), Query Frame = 0 

Query: 33  ICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFLNLIEKHL-QNSSTLPKSVT 92
           IC+F  +PSYC SVL    G++Y  GR S+RRSL++A  F+++I+  L +      KS  
Sbjct: 38  ICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKSTV 97

Query: 93  GGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLNTV 152
           G L+DC++LA L +++L +S +  + T K L+ S+A+ + + LSA +TN  TCL+GL + 
Sbjct: 98  GALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKST 157

Query: 153 ASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPV-------TKHLRFRK 212
           AS + L  D    L + TK Y +SL LF+KGWVPR   R R  P+        K   FR 
Sbjct: 158 ASENGLSGD----LFNDTKLYGVSLALFSKGWVPR---RQRSRPIWQPQARFKKFFGFRN 217

Query: 213 GPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVNAAPN 272
           G LPL+M++  RAVYN+V  RR+L  S  D + V+ IV V Q+GTGNF TI  A+ AAPN
Sbjct: 218 GKLPLKMTERARAVYNTV-TRRKLLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPN 277

Query: 273 NSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSAT 332
            +   NGYFLIY+ AG+Y+EYV +P  K+Y++MIGDGINQT+ITGN+SV DGWTTFNSAT
Sbjct: 278 KTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSAT 337

Query: 333 FAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFR 392
           F ++   F+ VNIT +NTAG  KGQAVA+RSG D+S+FYSCSFE YQDTLYTHSLRQF+R
Sbjct: 338 FILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYR 397

Query: 393 ECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRA 452
           ECD+YGTVDFIFGNAAVV QNCN+YPR PR GQ+N +TAQGR+DPNQNTGT+IH CTIR 
Sbjct: 398 ECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRP 457

Query: 453 TSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY-LSTLYYAEYN 512
             DLA S +Y  KTYLGRPWK+YSRTV MQT+IDGF+ P+GW+ WSG++ LSTLYYAEYN
Sbjct: 458 ADDLATS-NYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYN 517

Query: 513 NTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 557
           NTG GS+T NRVTWPGYHVI NATDASNFTV+NFLVG+ W+  TGVP+ GG I
Sbjct: 518 NTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

[Close]
BLAST of CmaCh01G016400 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 627.9 bits (1618), Expect = 8.0e-180
Identity = 330/574 (57.49%), Postives = 419/574 (73.00%), Query Frame = 0 

Query: 1   MALIASFFVLLTL-ISSSFAADGSLNDLFLAKAICKFTSNPSYCISVL-QKSSGNVYESG 60
           M  +  F V L L + + F A  +L     +  ICK T +P +C SV  Q S G+V E G
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYG 60

Query: 61  RFSMRRSLAKATTFLNLIEKHL-QNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNG 120
           RFS+R+SL ++  F   I+++L +N++ L +S  G L+DC+YLA L  ++L  SF  VN 
Sbjct: 61  RFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNI 120

Query: 121 T--DKKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSL 180
           T   K L++SKAD IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+
Sbjct: 121 TTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSV 180

Query: 181 SLDLFTKGWVPRNRNRTRD----HPVTKH------LRFRKGPLPLRMSKHDRAVYNSVAN 240
           SL LFTKGWVP+ + +       HP   H        FR G LPL+M++H RAVY S++ 
Sbjct: 181 SLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSR 240

Query: 241 RRRLSSSSD-DGILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVYQ 300
           R+     +D + +LV+ IV V+Q+GTGNF TITEAVN+APN +    GYF+IY+ +GVY+
Sbjct: 241 RKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYE 300

Query: 301 EYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNTA 360
           E V I   K+YL+MIGDGIN+T++TGN++V DGWTTFNSATFAV +  F+AVN+TF+NTA
Sbjct: 301 ENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTA 360

Query: 361 GAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVF 420
           G  K QAVAMRS AD+SIFYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVF
Sbjct: 361 GPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVF 420

Query: 421 QNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGRP 480
           Q+CN+YPR P   Q N ITAQGR+DPNQNTG SIHNCTI+   DL +S +Y  KTYLGRP
Sbjct: 421 QDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKTYLGRP 480

Query: 481 WKQYSRTVYMQTFIDGFVNPAGWDPWSGEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYHV 540
           WK+YSRTV+MQ++ID  V P GW  W+G++ LSTLYYAEYNNTG+GS+T +RV WPGYHV
Sbjct: 481 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 540

Query: 541 ISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 557
           I N+TDA+NFTV NFL+GD W+  +GVPY  G +
Sbjct: 541 I-NSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

[Close]
BLAST of CmaCh01G016400 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 621.7 bits (1602), Expect = 5.7e-178
Identity = 329/575 (57.22%), Postives = 412/575 (71.65%), Query Frame = 0 

Query: 8   FVLLTLISSSFAADGSLNDLFLAKAICKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLA 67
           F+L+TL     +   S   L  +  ICK T +P YC SV   S GNV + G FS+R+SL+
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 68  KATTFLNLIEKHLQNSSTLPK-SVTGGLKDCQYLAQLNINFLSNSFRAVNGTD-----KK 127
           ++  F+  ++++++ ++ L + +V   L+DC++LA L +++L  SF  VN T      K 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 128 LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS---SLEKDLLAPLIDCTKSYSLSLDL 187
           L++ KAD IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL L
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 188 FTKGWVPRNRN---------RTRDHPVTKHLR-FRKGPLPLRMSKHDRAVYNSVANRRRL 247
           FTKGWVP+ +          R+     TK  R FR G LPL+M++  +AVY S++ R+  
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 248 SSSS----DDG--ILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYFLIYIAAGVY 307
              S    DDG  +L++ IV VSQDGTGNF  IT AV AAPNN+    G+FLIY+ AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 308 QEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFMAVNITFQNT 367
           +EY+SI   K+Y++MIGDGINQT++TGN+SV DGWTTFNSATFAV A  F+AVNITF+NT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 368 AGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVV 427
           AG  K QAVA+RSGAD SIFYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 428 FQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKSYMNKTYLGR 487
           FQNCN+YPR P   Q N ITAQGRSDPNQNTGTSI NCTI+   DL +S +Y  KTYLGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSS-NYTVKTYLGR 485

Query: 488 PWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY-LSTLYYAEYNNTGAGSNTKNRVTWPGYH 547
           PWK+YSRTVYMQ++IDGFV P GW  W+G++ LSTLYYAEYNNTG GSNT NRVTWPGYH
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 548 VISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 557
           VI N+TDA+NFTV+   +   W+  TGVPYT G I
Sbjct: 546 VI-NSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

[Close]
BLAST of CmaCh01G016400 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 459.1 bits (1180), Expect = 4.9e-129
Identity = 252/545 (46.24%), Postives = 345/545 (63.30%), Query Frame = 0 

Query: 34  CKFTSNPSYCISVL---QKSSGNVYESGRFSMRRSLAKATTFLNLIEKHLQNSSTLPKSV 93
           CK T  P  C ++L   + S  + Y  G+F++++ L +A+    +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 94  T----GGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLD 153
           T    G + DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 154 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKGWVPRNRNRTRD 213
           GL  V + S     + +P+ + T+ YS+SL L              +KG +    N T  
Sbjct: 203 GL--VEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGGGNSTYR 262

Query: 214 HPVTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFL 273
            P+   ++  +     +   +D+    +  N   L  +S   ILV+  V+V    + NF 
Sbjct: 263 EPLETLIKGLR-----KTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFT 322

Query: 274 TITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSV 333
           TIT+A+ AAPNN+   +GYF+IY   GVY+EY+ +P  KK L+++GDGIN+TIITGN +V
Sbjct: 323 TITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNV 382

Query: 334 ADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDT 393
            DGWTT+N ++FAV  E FMAV++TF+NTAG  K QAVA+R+ A+ S FY CSFEGYQDT
Sbjct: 383 VDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDT 442

Query: 394 LYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNT 453
           LY HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P + Q N ITA GR DPNQNT
Sbjct: 443 LYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNT 502

Query: 454 GTSIHNCTIRATSDLAAS-KSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGE 513
           G SI NCTI+A  DLAA  KS M  T+LGRPWK YSRTV+MQ++I   V P GW  W+G 
Sbjct: 503 GISIINCTIKAAPDLAAEPKSAM--TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGT 562

Query: 514 Y-LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPY 557
             L T+YY EY+N G G+NT  RV W GY+++ N  +A NFTV NF +GD WLP T +P+
Sbjct: 563 IGLDTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQTDIPF 616

[Close]
BLAST of CmaCh01G016400 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 443.0 bits (1138), Expect = 3.6e-124
Identity = 256/544 (47.06%), Postives = 333/544 (61.21%), Query Frame = 0 

Query: 34  CKFTSNPSYC---ISVLQKSSGNVYESGRFSMRRSLAKATTFLNLIEKHLQNSSTLPKSV 93
           CK T  P  C   +S ++ S  + Y  G+F+M++ L +A     +I +  Q     P + 
Sbjct: 88  CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEADPGTS 147

Query: 94  T----GGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLD 153
           T      + DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN  TCLD
Sbjct: 148 TVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQQTCLD 207

Query: 154 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHLRFRKGP 213
           GL    SG +    +  PL + T+ YS+SL L +      NRN  R +  +K   F  G 
Sbjct: 208 GLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA---LNRNLKR-YKGSKGKIFGGGN 267

Query: 214 LPLR----------MSKHDRAVYNSVANRR--RLSSSSDDGILVNGIVVVSQDGTGNFLT 273
            P+R              D+      ANR    L  +S   ILV   V V    T NF T
Sbjct: 268 KPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDNFPT 327

Query: 274 ITEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVA 333
           ITEAV AAPN++    GYF+IY  AG+Y+EYV I +KK+ +++IGDGIN+TII+GN S  
Sbjct: 328 ITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSFI 387

Query: 334 DGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTL 393
           DGWTT+NS+TFAV  + F+AV++TF+NTAG  K QAVA+R+ AD S FY CSFEGYQDTL
Sbjct: 388 DGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTL 447

Query: 394 YTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTG 453
           Y HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P + Q N +TA GR+DPNQ TG
Sbjct: 448 YVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTG 507

Query: 454 TSIHNCTIRATSDLAAS-KSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEY 513
            SI NCTI A  DLAA  KS M  T+LGRPWK YSRTVY+Q++I   V P GW  W+G  
Sbjct: 508 ISIINCTIGAAPDLAADPKSTM--TFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTT 567

Query: 514 -LSTLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYT 557
            L T+ Y EY+N G G++T  RV W GY ++ N   A NFTV NF +GD WLP T +P+ 
Sbjct: 568 GLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQTDIPFY 621

The following BLAST results are available for this feature:
BLAST of CmaCh01G016400 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O222564.1e-18159.85Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK71.1e-17857.49Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX48.0e-17757.22Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q94CB16.9e-12846.24Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF775.1e-12347.06Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
BLAST of CmaCh01G016400 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1J3230.0e+0098.90Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480894 PE=3 SV=1[more]
A0A6J1FJR94.5e-30897.42Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111446371 PE=3 SV=1[more]
A0A5A7TAP22.3e-28387.50Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00574... [more]
A0A1S4E6G92.3e-28387.50Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1[more]
A0A0A0LHM08.6e-28386.87Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G890080 PE=3 SV=1[more]
BLAST of CmaCh01G016400 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022981882.10.0e+0098.90probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita maxima... [more]
KAG6608276.16.4e-30997.97putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
XP_023524144.11.1e-30897.79probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo s... [more]
XP_022940931.19.4e-30897.42probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita moscha... [more]
XP_016903565.14.7e-28387.50PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucum... [more]
BLAST of CmaCh01G016400 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G47550.12.9e-18259.85Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.18.0e-18057.49Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.15.7e-17857.22Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.24.9e-12946.24Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.13.6e-12447.06Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 25..178
e-value: 1.2E-16
score: 71.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..158
e-value: 5.9E-16
score: 59.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 3..152
e-value: 2.7E-10
score: 37.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 242..541
e-value: 5.0E-135
score: 449.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 29..181
e-value: 2.1E-19
score: 72.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 31..181
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 238..555
e-value: 3.4E-121
score: 406.5
NoneNo IPR availablePANTHERPTHR31707:SF188PECTINESTERASEcoord: 11..554
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 11..554
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 32..183
e-value: 7.11784E-27
score: 104.442
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 238..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G016400.1CmaCh01G016400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity