CmaCh01G016310 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G016310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionserine/threonine-protein kinase PEPKR2-like
LocationCma_Chr01: 11178786 .. 11182195 (+)
RNA-Seq ExpressionCmaCh01G016310
SyntenyCmaCh01G016310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGAAAAGGAAAGGAACCGAGACTGATAATGGACAACCTTTGGACTTACCAGATAATGTGACAGAAACTCGTGATTCGCGATCTTCTAAACTGAAGGCTACTTACTCGCTAGTAGATTACACGAGGTTGAATAAAAGGTGCAAGGAGAATGTGGGTATTGAACCAGTTCCTTCATGTAAGAGTAGGTTTGCAGCCATAGCTACAGCCCCACCTATTGGGAGTTCGTCTCTGATTCCTCCTGTTAGAGGTTTGAAGAGGAAGATTGGATGCATAGACGTGGCTACGCAAATTGGGCGAAAACGTAACATCGAAAATGATTATGTTTCAGGCGAGACCATTGGGCATGGAAAATTTGGTTCAGTTTGGCTGTGTAAATGTAAGCTTACTGGAGCTGAGTATGCTTGCAAAACTCTGAAAAAAGGAGAAGAGACTGTGCATAAGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCGAGTGTTGTGACATTGCTGGCAGTGTATGAGGAGTCAGAATGTTTTCACCTGGTTATGGAACTGTGTCGTGGAGGTCGTTTGGTTGATCAGATGGTCAGTGAAGGTCAATATTCGGAGCATCGAGCAGCTAATATACTCAAGGAAGTGATGTTGGTTATAAAGTATTGTCATGATATGGGGGTTGTACATCGAGACATAAAGCCCGAAAATATTCTTCTCACGACGTCTGGGAAGATCAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATGTGGTATGTATCCTTAAATCTGTACTAGAGAGTTCGTGTTTATATGTTCCCCTTTTGGTATTAGTTACCTTAACTTCCTTTAGTCTTCACTTGACACTATGAGAAAACTAATTTTGTTGGCATGATGTGAGAAAAGGGAGAACCATCTTGGGTTTAGAAGCTGTGAAGGTGTGAAGTTTGGCTCTTTTTAACGCCTTCATTTGGGTTCAAACGTTGAAACTTTTTTTTTTTGTAGACATTCAATGTTTCTTCTGATCACCAACAATTTTTATTCATCTTTTGGTTCCATTTGCTGGTTTGGTTCCTGCTTTTGTTGACATTTTTTTTTTTTTTTTTAATTTCTTTTCAATGAAAGCTGAAAGCTGATTTCAGAATAAAAATAGGATAAGATCAAATATCTGAAGCTATTAATCAACATGTGAGAGGGAAGGGATTATTCTTGTGTCTGTGATGTGCTTGGATTGTTGTTCACAAGGTAATCTTTCAATGAGATTATTGTGTAAATATTTGTGACTGTAGTTAGGAGTGATCGTGCTGATGTCTTATTAGAAAATAAGTGAGGAATGTCTCTACGAATAAGAATACAGAGTTTATAGACTGAACAAGACAACAAGATGACACGCAGGAGTCTGTGACAAGGTTGCAAAGGAAGTCGATTTTTTTGGTAAGTTATGGAAAGATGAGTCATATAATTATGTTAGAGGACTAGTTTTGTAACGACCCAAGTCCCCACCGCTAGCTGATATTGTTCTCTTTTAGTTTTTCCTTTTGGGCTTACTCTCAAAGTTTTTAAAACGTGTCTGTTAGGGAGAAGTTTCCACCCCCTTATAAAGAATACTTCGTTCTCCTCCCCAACCGACGTGGGATCACACAAGTTTTGTAGTGTTTTTTTATCTTTCTTTTCCTCTTTAGGACCTTTCATCTAACTACGAGAGCAGATTCCGTAATGTGAATGGCGTGACCGTTGGAGTCCTTTGATGAGATGAAATATGCACAAGAAAAAAAAGAAATCCCAAGACCAACTTAAGGAAGATACTAAATATTCCAATTAGCAAAAGCAGAAGTCAAATTATAGCCACAAAAAGTAGTTTTTTGATCTTACACCAAGCAGCTTAGACAAAACAACCCAACCCCATTAGTCCTCGAGGTCGAAAAAGAACAAACCGTAGCTGACACTGCTGGTCTTTAACTTAAGCAACAGTGGACACATTATCGTATTAGCTACAATAGTGTTAGTCTTAGAGAGAATGAGAGAGGATGCTTATGGGGGAGAGGGGGGGTAATTTGGTTCGGTATCACGAATCGGAGAAGGAAGTCTTAATTGTGGATGGTAAAATGATATGGTAAACTGCTTCTTCGTAAAAACTACAGAGGTTTTTGAGTTTGTAGTTGTTTTCTCTTTATGTATTATTCTTCTTATAAGCTTTTACTTGATCTGCTATAGTTTGTGTGAATGTTGCTTTGTTTTATACTTAGGATTATGTTTCAAACATTTGCTTAGTTAAGTTATTTGATTGCGGACTGCAGGCCAGAGTTTAACTGGTCTGGCTGGAAGCCCAGCTTATGTGGCCCCTGAAGTGTTGTCGGGAAATTACTCTGAGAAGGTGGATATTTGGAGTGCTGGTGTGCTCCTCCATGCTCTTTTGGTTGCCACTCTTCCTTTCCAAGGCGATTCGGTAGAATCAGTTTTTGAAGCAATAAAGAACACCAAGCTCGACTTCCACTCCGGGATGTGGGAGTCCATATCTAAACCTGCTCGAGATCTCATCGAGGGAATGTTGACGAGAGATATTTCAGCTAGGATAACGGCAGAAGAAGTTTTAAGTAAGTTCGTTTTCCTAATTGATTTCTAAGCAATAGTAATATGTTATCAATGAACTTTGCAATTAGAATATAGGTTAATATCAATCCTTCTTACGTAATATAATCTCATTTGGATACCAATGGTCATACTTCAGGGCATCCCTGGATATTGTTTTACACCGAACGCACGTTCAAGACGCTTCCCGTCAAACTGAAACTGAAGAATCAAGAATCTTGCCAACAGTCTCCCATTGCAGCCAAAATTAAGCCTAACAGAAACAGATTTGACAGTGCAGCTATTGCTGCCTCCCTTAGTGAAGTCTCTAATCTTTCATCATCTGAAAATTCTAATGCCAACAGTGACGATGTCGACGACTCCTTTCTGGTGGACGCACTTGCAACAGCTATTTCACACGTGACAATTTCCGAGCCAAAGAGAAGCAGGCTGTGTGTTCCTACAGGCCGTATTGAGCAGCACAGCTCCTCTAACATGAAAGCTAACAATCTCTGTAAAGCGTTTTGATCCTGCGAGTGTGCCAACGTTGTCGTCGACATTGTTCTCTCCTGTTTTTTTTCCTAAGCTGGGGAACTACTAGTACTTACAGGTAACACAACCGGGATGAATTTCTCCAAGTAGCTAAGAGGGTGATTTCCAGGCCAACATCAACGAAATTTAACGAGTTTTATTAATATGCACTCTATGTTAGTCAATGCCTGAGGTGCATTAAATCTGATTAGAAGTGTGTGATTTTGTGCTTTAAGTCTAATAATTGTCTTGACATGAATTGACAAATTTGATGTACATATTTTGTAAATACAGCAGTAATGAGAAGAGAGGGCATTTTGATTT

mRNA sequence

ATGAGGAAGAAAAGGAAAGGAACCGAGACTGATAATGGACAACCTTTGGACTTACCAGATAATGTGACAGAAACTCGTGATTCGCGATCTTCTAAACTGAAGGCTACTTACTCGCTAGTAGATTACACGAGGTTGAATAAAAGGTGCAAGGAGAATGTGGGTATTGAACCAGTTCCTTCATGTAAGAGTAGGTTTGCAGCCATAGCTACAGCCCCACCTATTGGGAGTTCGTCTCTGATTCCTCCTGTTAGAGGTTTGAAGAGGAAGATTGGATGCATAGACGTGGCTACGCAAATTGGGCGAAAACGTAACATCGAAAATGATTATGTTTCAGGCGAGACCATTGGGCATGGAAAATTTGGTTCAGTTTGGCTGTGTAAATGTAAGCTTACTGGAGCTGAGTATGCTTGCAAAACTCTGAAAAAAGGAGAAGAGACTGTGCATAAGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCGAGTGTTGTGACATTGCTGGCAGTGTATGAGGAGTCAGAATGTTTTCACCTGGTTATGGAACTGTGTCGTGGAGGTCGTTTGGTTGATCAGATGGTCAGTGAAGGTCAATATTCGGAGCATCGAGCAGCTAATATACTCAAGGAAGTGATGTTGGTTATAAAGTATTGTCATGATATGGGGGTTGTACATCGAGACATAAAGCCCGAAAATATTCTTCTCACGACGTCTGGGAAGATCAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATGTGGCCAGAGTTTAACTGGTCTGGCTGGAAGCCCAGCTTATGTGGCCCCTGAAGTGTTGTCGGGAAATTACTCTGAGAAGGTGGATATTTGGAGTGCTGGTGTGCTCCTCCATGCTCTTTTGGTTGCCACTCTTCCTTTCCAAGGCGATTCGGTAGAATCAGTTTTTGAAGCAATAAAGAACACCAAGCTCGACTTCCACTCCGGGATGTGGGAGTCCATATCTAAACCTGCTCGAGATCTCATCGAGGGAATGTTGACGAGAGATATTTCAGCTAGGATAACGGCAGAAGAAGTTTTAAGGCATCCCTGGATATTGTTTTACACCGAACGCACGTTCAAGACGCTTCCCGTCAAACTGAAACTGAAGAATCAAGAATCTTGCCAACAGTCTCCCATTGCAGCCAAAATTAAGCCTAACAGAAACAGATTTGACAGTGCAGCTATTGCTGCCTCCCTTAGTGAAGTCTCTAATCTTTCATCATCTGAAAATTCTAATGCCAACAGTGACGATGTCGACGACTCCTTTCTGGTGGACGCACTTGCAACAGCTATTTCACACGTGACAATTTCCGAGCCAAAGAGAAGCAGGCTGTGTGTTCCTACAGGCCGTATTGAGCAGCACAGCTCCTCTAACATGAAAGCTAACAATCTCTGTAAAGCGTTTTGATCCTGCGAGTGTGCCAACGTTGTCGTCGACATTGTTCTCTCCTGTTTTTTTTCCTAAGCTGGGGAACTACTAGTACTTACAGGTAACACAACCGGGATGAATTTCTCCAAGTAGCTAAGAGGGTGATTTCCAGGCCAACATCAACGAAATTTAACGAGTTTTATTAATATGCACTCTATGTTAGTCAATGCCTGAGGTGCATTAAATCTGATTAGAAGTGTGTGATTTTGTGCTTTAAGTCTAATAATTGTCTTGACATGAATTGACAAATTTGATGTACATATTTTGTAAATACAGCAGTAATGAGAAGAGAGGGCATTTTGATTT

Coding sequence (CDS)

ATGAGGAAGAAAAGGAAAGGAACCGAGACTGATAATGGACAACCTTTGGACTTACCAGATAATGTGACAGAAACTCGTGATTCGCGATCTTCTAAACTGAAGGCTACTTACTCGCTAGTAGATTACACGAGGTTGAATAAAAGGTGCAAGGAGAATGTGGGTATTGAACCAGTTCCTTCATGTAAGAGTAGGTTTGCAGCCATAGCTACAGCCCCACCTATTGGGAGTTCGTCTCTGATTCCTCCTGTTAGAGGTTTGAAGAGGAAGATTGGATGCATAGACGTGGCTACGCAAATTGGGCGAAAACGTAACATCGAAAATGATTATGTTTCAGGCGAGACCATTGGGCATGGAAAATTTGGTTCAGTTTGGCTGTGTAAATGTAAGCTTACTGGAGCTGAGTATGCTTGCAAAACTCTGAAAAAAGGAGAAGAGACTGTGCATAAGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCGAGTGTTGTGACATTGCTGGCAGTGTATGAGGAGTCAGAATGTTTTCACCTGGTTATGGAACTGTGTCGTGGAGGTCGTTTGGTTGATCAGATGGTCAGTGAAGGTCAATATTCGGAGCATCGAGCAGCTAATATACTCAAGGAAGTGATGTTGGTTATAAAGTATTGTCATGATATGGGGGTTGTACATCGAGACATAAAGCCCGAAAATATTCTTCTCACGACGTCTGGGAAGATCAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATGTGGCCAGAGTTTAACTGGTCTGGCTGGAAGCCCAGCTTATGTGGCCCCTGAAGTGTTGTCGGGAAATTACTCTGAGAAGGTGGATATTTGGAGTGCTGGTGTGCTCCTCCATGCTCTTTTGGTTGCCACTCTTCCTTTCCAAGGCGATTCGGTAGAATCAGTTTTTGAAGCAATAAAGAACACCAAGCTCGACTTCCACTCCGGGATGTGGGAGTCCATATCTAAACCTGCTCGAGATCTCATCGAGGGAATGTTGACGAGAGATATTTCAGCTAGGATAACGGCAGAAGAAGTTTTAAGGCATCCCTGGATATTGTTTTACACCGAACGCACGTTCAAGACGCTTCCCGTCAAACTGAAACTGAAGAATCAAGAATCTTGCCAACAGTCTCCCATTGCAGCCAAAATTAAGCCTAACAGAAACAGATTTGACAGTGCAGCTATTGCTGCCTCCCTTAGTGAAGTCTCTAATCTTTCATCATCTGAAAATTCTAATGCCAACAGTGACGATGTCGACGACTCCTTTCTGGTGGACGCACTTGCAACAGCTATTTCACACGTGACAATTTCCGAGCCAAAGAGAAGCAGGCTGTGTGTTCCTACAGGCCGTATTGAGCAGCACAGCTCCTCTAACATGAAAGCTAACAATCTCTGTAAAGCGTTTTGA

Protein sequence

MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPSCKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFYTERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNANSDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF
Homology
BLAST of CmaCh01G016310 vs. ExPASy Swiss-Prot
Match: Q8W490 (Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2 PE=2 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 1.6e-162
Identity = 302/479 (63.05%), Postives = 371/479 (77.45%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKG+ET+  +  +LPD+++ +  SRSS  ++ +SL  Y RL KRCKE   +E V S
Sbjct: 1   MRKKRKGSETEGWE--NLPDDLSCSTASRSSNFRSHFSLEGYARLKKRCKETEAVESVGS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
            K R A +ATAPP G+SSL+   RGLKRKIGCIDV+TQ GRK  I++DYV G  IG GKF
Sbjct: 61  FKRRIAGVATAPPCGASSLVSSGRGLKRKIGCIDVSTQTGRKNKIDDDYVFGRNIGKGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSV +CK +  G E+ACKTLKKGEETVH+EVEIMQHLSG+P VVTL AVYEES+CFHLVM
Sbjct: 121 GSVRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVVTLHAVYEESDCFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELC GGRL+DQMV  G+YSE RAANI K++MLVI YCH+MGVVHRDIKPENILLT +GKI
Sbjct: 181 ELCSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           +LADFGLA RI+ GQ+L+GLAGSPAYVAPEVLS NYSEKVD+WSAGVLL+ALL   LPF+
Sbjct: 241 QLADFGLAMRIAKGQTLSGLAGSPAYVAPEVLSENYSEKVDVWSAGVLLYALLSGVLPFK 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDS++++FEAIKN KLDF++G+WES+SKPARDL+  MLTR+ SARITA+EVLRHPWILFY
Sbjct: 301 GDSLDAIFEAIKNVKLDFNTGVWESVSKPARDLLARMLTREESARITADEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQ---ESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENS 420
           T+RT KT+ +K K K+Q    +C Q+    + K + NR D      S S   + S++E  
Sbjct: 361 TDRTLKTMCIKSKHKSQAGSSTCLQNRSPTE-KTDLNRADREKKIPSDSPTDSFSNTE-- 420

Query: 421 NANSDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGR-IEQHSSSNM-KANNLCKAF 475
               ++ D+S +VD L  AI++V ISEPKRSR+C PT   IEQ  SSN+   + LC+AF
Sbjct: 421 ----EEEDESGVVDVLVVAIANVRISEPKRSRVCSPTNNPIEQQHSSNLTSTSTLCRAF 470

BLAST of CmaCh01G016310 vs. ExPASy Swiss-Prot
Match: Q6F3A6 (Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK10 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.4e-65
Identity = 119/288 (41.32%), Postives = 183/288 (63.54%), Query Frame = 0

Query: 80  IPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKT 139
           +PPV+ +    G +  +    +  ++++ Y  G  +G G+FG+ +LC  + TG E+ACK+
Sbjct: 105 VPPVKRVS-SAGLLVGSVLKRKTESLKDKYSLGRKLGQGQFGTTYLCVERATGKEFACKS 164

Query: 140 LKKGE-------ETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQM 199
           + K +       E V +E++IM HL+G+P+V+++   YE++   HLVMELC GG L D++
Sbjct: 165 ILKRKLVTDDDVEDVRREIQIMYHLAGHPNVISIRGAYEDAVAVHLVMELCAGGELFDRI 224

Query: 200 VSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILL---TTSGKIKLADFGLAT 259
           V +G Y+E +AA + + ++ V++ CH MGV+HRD+KPEN L    T    +K  DFGL+ 
Sbjct: 225 VQKGHYTERKAAELARVIVGVVEVCHSMGVMHRDLKPENFLFADQTEEAALKTIDFGLSI 284

Query: 260 RISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFE 319
               GQ  T + GSP YVAPEVL   Y ++ D+WSAGV+++ LL    PF  ++ + +FE
Sbjct: 285 FFRPGQVFTDVVGSPYYVAPEVLKKKYGQEADVWSAGVIIYILLCGVPPFWAENEQGIFE 344

Query: 320 AIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWI 358
            + + +LDF S  W SIS+ A+DL+  ML RD   R+TA EVLRHPW+
Sbjct: 345 EVLHGRLDFQSEPWPSISEGAKDLVRRMLVRDPKKRLTAHEVLRHPWV 391

BLAST of CmaCh01G016310 vs. ExPASy Swiss-Prot
Match: Q9SJ61 (Calcium-dependent protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CPK25 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.6e-64
Identity = 116/264 (43.94%), Postives = 174/264 (65.91%), Query Frame = 0

Query: 104 NIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTLKKGE-------ETVHKEVEIMQH 163
           +++  Y  G  +GHG+FG+ ++C  K TG EYACK++ K +       E V +E+EIM+H
Sbjct: 127 HLKEYYNLGSKLGHGQFGTTFVCVEKGTGEEYACKSIPKRKLENEEDVEDVRREIEIMKH 186

Query: 164 LSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKY 223
           L G P+V+++   YE+S   H+VMELCRGG L D++V  G YSE +AA++ K ++ V++ 
Sbjct: 187 LLGQPNVISIKGAYEDSVAVHMVMELCRGGELFDRIVERGHYSERKAAHLAKVILGVVQT 246

Query: 224 CHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISCGQSLTGLAGSPAYVAPEVLS 283
           CH +GV+HRD+KPEN L     +   +K  DFGL+  +  G++ T + GSP Y+APEVL+
Sbjct: 247 CHSLGVMHRDLKPENFLFVNDDEDSPLKAIDFGLSMFLKPGENFTDVVGSPYYIAPEVLN 306

Query: 284 GNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSGMWESISKPARDL 343
            NY  + DIWSAGV+++ LL  + PF G++ E +F  +   +LD  S  W  +S+ A+DL
Sbjct: 307 KNYGPEADIWSAGVMIYVLLSGSAPFWGETEEEIFNEVLEGELDLTSDPWPQVSESAKDL 366

Query: 344 IEGMLTRDISARITAEEVLRHPWI 358
           I  ML R+   R+TA++VL HPWI
Sbjct: 367 IRKMLERNPIQRLTAQQVLCHPWI 390

BLAST of CmaCh01G016310 vs. ExPASy Swiss-Prot
Match: Q9ZV15 (Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.3e-63
Identity = 115/275 (41.82%), Postives = 177/275 (64.36%), Query Frame = 0

Query: 93  IDVATQIGRK-RNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTLKKGE------- 152
           + + + +GRK  N+++ Y  G  +G G+FG+ +LC  K TG E+ACKT+ K +       
Sbjct: 117 LQIDSVLGRKTENLKDIYSVGRKLGQGQFGTTFLCVDKKTGKEFACKTIAKRKLTTPEDV 176

Query: 153 ETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAA 212
           E V +E++IM HLSG+P+V+ ++  YE++   H+VME+C GG L D+++  G Y+E +AA
Sbjct: 177 EDVRREIQIMHHLSGHPNVIQIVGAYEDAVAVHVVMEICAGGELFDRIIQRGHYTEKKAA 236

Query: 213 NILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISCGQSLTGLA 272
            + + ++ VI+ CH +GV+HRD+KPEN L  +  +   +K  DFGL+     G++ T + 
Sbjct: 237 ELARIIVGVIEACHSLGVMHRDLKPENFLFVSGDEEAALKTIDFGLSVFFKPGETFTDVV 296

Query: 273 GSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSG 332
           GSP YVAPEVL  +YS + D+WSAGV+++ LL    PF  ++ + +FE +    LDF S 
Sbjct: 297 GSPYYVAPEVLRKHYSHECDVWSAGVIIYILLSGVPPFWDETEQGIFEQVLKGDLDFISE 356

Query: 333 MWESISKPARDLIEGMLTRDISARITAEEVLRHPW 357
            W S+S+ A+DL+  ML RD   R+T  EVL HPW
Sbjct: 357 PWPSVSESAKDLVRRMLIRDPKKRMTTHEVLCHPW 391

BLAST of CmaCh01G016310 vs. ExPASy Swiss-Prot
Match: P53684 (Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK7 PE=2 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 3.9e-63
Identity = 120/270 (44.44%), Postives = 168/270 (62.22%), Query Frame = 0

Query: 99  IGRK-RNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTL-------KKGEETVHKE 158
           +GRK  ++   Y+ G  +G G+FG+ +LC    TG EYACKT+       K+  E V +E
Sbjct: 68  LGRKTADLREHYIIGRKLGQGQFGTTYLCTEINTGCEYACKTIPKRKLITKEDVEDVRRE 127

Query: 159 VEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAANILKEV 218
           ++IM HLSG+ +VV +  VYE+ +  H+VMELC GG L D++  +G YSE +AA +++ +
Sbjct: 128 IQIMHHLSGHKNVVAIKDVYEDGQAVHIVMELCAGGELFDRIQEKGHYSERKAAELIRII 187

Query: 219 MLVIKYCHDMGVVHRDIKPENILLTTSG---KIKLADFGLATRISCGQSLTGLAGSPAYV 278
           + ++  CH +GV+HRD+KPEN LL        IK  DFGL+     GQ  T L GSP YV
Sbjct: 188 VSIVAMCHSLGVMHRDLKPENFLLLDKDDDLSIKAIDFGLSVFFKPGQVFTELVGSPYYV 247

Query: 279 APEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSGMWESIS 338
           APEVL   Y  + D+WSAGV+L+ LL    PF  ++ + +F+A+    +DF S  W  IS
Sbjct: 248 APEVLHKRYGPESDVWSAGVILYVLLSGVPPFWAETQQGIFDAVLKGHIDFQSDPWPKIS 307

Query: 339 KPARDLIEGMLTRDISARITAEEVLRHPWI 358
             A+DLI  ML+   S R+ A EVLRHPWI
Sbjct: 308 DSAKDLIRKMLSHCPSERLKAHEVLRHPWI 337

BLAST of CmaCh01G016310 vs. ExPASy TrEMBL
Match: A0A6J1ISH9 (serine/threonine-protein kinase PEPKR2-like OS=Cucurbita maxima OX=3661 GN=LOC111480208 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.7e-269
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS
Sbjct: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ
Sbjct: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN
Sbjct: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
           SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF
Sbjct: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 474

BLAST of CmaCh01G016310 vs. ExPASy TrEMBL
Match: A0A6J1FL12 (serine/threonine-protein kinase PEPKR2-like OS=Cucurbita moschata OX=3662 GN=LOC111446379 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.1e-265
Identity = 467/474 (98.52%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKGTETDNGQPLDLP+NVT TRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS
Sbjct: 1   MRKKRKGTETDNGQPLDLPENVTGTRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ
Sbjct: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDSVE+VFEAIKN KLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSVEAVFEAIKNAKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERTFKTLPVKLK KNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN
Sbjct: 361 TERTFKTLPVKLKPKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
            DDVDDSFLVDALATAISHVTISEPKRSRLCVPTG IEQHSSSNMKANNLCKAF
Sbjct: 421 GDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGHIEQHSSSNMKANNLCKAF 474

BLAST of CmaCh01G016310 vs. ExPASy TrEMBL
Match: A0A5D3E5W2 (Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005900 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.5e-235
Identity = 421/474 (88.82%), Postives = 439/474 (92.62%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKG+ETD   P +L DNVTE+ DSRSSKLKA YSL DYTRLNKRCKE+VGIEPV S
Sbjct: 1   MRKKRKGSETDG--PSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFA IATAPP GSSSLI P RGLKRKIGCIDVATQIGRK NIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCK++GAEYACKTLKKGEETVH+EVEIMQHLSGYP VVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQM SEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRIS GQSLTGLAGSPAYVAPEVL+G Y+EKVDIWSAGVLLHALLV  LPFQ
Sbjct: 241 KLADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDS+ESVFEAIKN+KLDFHSGMWESISKPARDLI  MLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERT K LP+KLKLKNQES QQSPIA+KIK +RNR DSAA   SL+E+SNLSSSE+ NA+
Sbjct: 361 TERTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNAD 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
            DDVDD FLVDALATAISHV ISEPKRSRLCVPTG IEQHSSSNMKANNLCKAF
Sbjct: 421 GDDVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of CmaCh01G016310 vs. ExPASy TrEMBL
Match: A0A1S3CQM3 (serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.5e-235
Identity = 421/474 (88.82%), Postives = 439/474 (92.62%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKG+ETD   P +L DNVTE+ DSRSSKLKA YSL DYTRLNKRCKE+VGIEPV S
Sbjct: 1   MRKKRKGSETDG--PSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFA IATAPP GSSSLI P RGLKRKIGCIDVATQIGRK NIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCK++GAEYACKTLKKGEETVH+EVEIMQHLSGYP VVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQM SEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRIS GQSLTGLAGSPAYVAPEVL+G Y+EKVDIWSAGVLLHALLV  LPFQ
Sbjct: 241 KLADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDS+ESVFEAIKN+KLDFHSGMWESISKPARDLI  MLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERT K LP+KLKLKNQES QQSPIA+KIK +RNR DSAA   SL+E+SNLSSSE+ NA+
Sbjct: 361 TERTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNAD 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
            DDVDD FLVDALATAISHV ISEPKRSRLCVPTG IEQHSSSNMKANNLCKAF
Sbjct: 421 GDDVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of CmaCh01G016310 vs. ExPASy TrEMBL
Match: A0A6J1IGJ4 (serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC111476741 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.3e-234
Identity = 420/474 (88.61%), Postives = 439/474 (92.62%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKG+ETD   PL+L DNVTE+ DSRSSKLKA YSL DYTRLNKRCKE+VGIEPVPS
Sbjct: 1   MRKKRKGSETDG--PLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFA IATAPP GSSSLI P RGLKRKIGCIDVATQIGRK NIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCK++GAEYACKTLKK EETVH+EVEIMQHLSGYP VVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQMVSEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRIS GQSL GLAGSPAYVAPEVLSG YSEKVDIWSAGVLLHALLV  LPFQ
Sbjct: 241 KLADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDS+ESVFEAIKN+KLDFHSGMWESISKPARDLI  MLTRDIS+RITAEEVLRHPWILFY
Sbjct: 301 GDSLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERT KTLP+KLKLKNQESCQ SPI+AK K +RNR DSAA   SL+EVS+LSSSE+ NAN
Sbjct: 361 TERTLKTLPIKLKLKNQESCQPSPISAKTKSDRNRIDSAANLTSLNEVSDLSSSESCNAN 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
            +D +D FLVDALATAISHV ISEPKRSRLCVPTG IEQHSSSNMKANNLCKAF
Sbjct: 421 GEDDEDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of CmaCh01G016310 vs. NCBI nr
Match: XP_022980892.1 (serine/threonine-protein kinase PEPKR2-like [Cucurbita maxima])

HSP 1 Score: 937.2 bits (2421), Expect = 5.6e-269
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS
Sbjct: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ
Sbjct: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN
Sbjct: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
           SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF
Sbjct: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 474

BLAST of CmaCh01G016310 vs. NCBI nr
Match: KAG7037610.1 (Serine/threonine-protein kinase PEPKR2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 927.5 bits (2396), Expect = 4.4e-266
Identity = 469/474 (98.95%), Postives = 471/474 (99.37%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKGTETDNGQPLDLP+NVT TRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS
Sbjct: 1   MRKKRKGTETDNGQPLDLPENVTGTRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ
Sbjct: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDSVE+VFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSVEAVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERTFKTLPVKLK KNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN
Sbjct: 361 TERTFKTLPVKLKPKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
            DDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF
Sbjct: 421 GDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 474

BLAST of CmaCh01G016310 vs. NCBI nr
Match: XP_022940947.1 (serine/threonine-protein kinase PEPKR2-like [Cucurbita moschata])

HSP 1 Score: 923.7 bits (2386), Expect = 6.4e-265
Identity = 467/474 (98.52%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKGTETDNGQPLDLP+NVT TRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS
Sbjct: 1   MRKKRKGTETDNGQPLDLPENVTGTRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ
Sbjct: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDSVE+VFEAIKN KLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSVEAVFEAIKNAKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420
           TERTFKTLPVKLK KNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN
Sbjct: 361 TERTFKTLPVKLKPKNQESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN 420

Query: 421 SDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
            DDVDDSFLVDALATAISHVTISEPKRSRLCVPTG IEQHSSSNMKANNLCKAF
Sbjct: 421 GDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGHIEQHSSSNMKANNLCKAF 474

BLAST of CmaCh01G016310 vs. NCBI nr
Match: XP_023524190.1 (serine/threonine-protein kinase PEPKR2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915.6 bits (2365), Expect = 1.7e-262
Identity = 468/479 (97.70%), Postives = 472/479 (98.54%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKGTETDNG+PLDLP+NVT TRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS
Sbjct: 1   MRKKRKGTETDNGKPLDLPENVTGTRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
           CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF
Sbjct: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM
Sbjct: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI
Sbjct: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSG+YSEKVDIWSAGVLLHALLVA LPFQ
Sbjct: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGSYSEKVDIWSAGVLLHALLVAALPFQ 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDSVESVFEAIKNTKLDFHSGMWESISKPARDLI+GMLTRDISARITAEEVLRHPWILFY
Sbjct: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIKGMLTRDISARITAEEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQESCQQSPI-AAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNA 420
           TERTFKTLPVKLKLKNQESCQQSPI AAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNA
Sbjct: 361 TERTFKTLPVKLKLKNQESCQQSPIAAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNA 420

Query: 421 NSD----DVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
           NSD    DVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF
Sbjct: 421 NSDDVDVDVDDSFLVDALATAISHVTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 479

BLAST of CmaCh01G016310 vs. NCBI nr
Match: KAG6608267.1 (Serine/threonine-protein kinase PEPKR2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 889.8 bits (2298), Expect = 1.0e-254
Identity = 450/455 (98.90%), Postives = 452/455 (99.34%), Query Frame = 0

Query: 20  DNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPSCKSRFAAIATAPPIGSSSL 79
           +NVT TRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPSCKSRFAAIATAPPIGSSSL
Sbjct: 24  ENVTGTRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPSCKSRFAAIATAPPIGSSSL 83

Query: 80  IPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKT 139
           IPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKT
Sbjct: 84  IPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKT 143

Query: 140 LKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYS 199
           LKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYS
Sbjct: 144 LKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYS 203

Query: 200 EHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKLADFGLATRISCGQSLTG 259
           EHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKLADFGLATRISCGQSLTG
Sbjct: 204 EHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKLADFGLATRISCGQSLTG 263

Query: 260 LAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFH 319
           LAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVE+VFEAIKNTKLDFH
Sbjct: 264 LAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVEAVFEAIKNTKLDFH 323

Query: 320 SGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFYTERTFKTLPVKLKLKNQES 379
           SGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFYTERTFKTLPVKLK KNQES
Sbjct: 324 SGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFYTERTFKTLPVKLKPKNQES 383

Query: 380 CQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNANSDDVDDSFLVDALATAISH 439
           CQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNAN DDVDDSFLVDALATAISH
Sbjct: 384 CQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENSNANGDDVDDSFLVDALATAISH 443

Query: 440 VTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 475
           VTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF
Sbjct: 444 VTISEPKRSRLCVPTGRIEQHSSSNMKANNLCKAF 478

BLAST of CmaCh01G016310 vs. TAIR 10
Match: AT1G12680.1 (phosphoenolpyruvate carboxylase-related kinase 2 )

HSP 1 Score: 573.9 bits (1478), Expect = 1.2e-163
Identity = 302/479 (63.05%), Postives = 371/479 (77.45%), Query Frame = 0

Query: 1   MRKKRKGTETDNGQPLDLPDNVTETRDSRSSKLKATYSLVDYTRLNKRCKENVGIEPVPS 60
           MRKKRKG+ET+  +  +LPD+++ +  SRSS  ++ +SL  Y RL KRCKE   +E V S
Sbjct: 1   MRKKRKGSETEGWE--NLPDDLSCSTASRSSNFRSHFSLEGYARLKKRCKETEAVESVGS 60

Query: 61  CKSRFAAIATAPPIGSSSLIPPVRGLKRKIGCIDVATQIGRKRNIENDYVSGETIGHGKF 120
            K R A +ATAPP G+SSL+   RGLKRKIGCIDV+TQ GRK  I++DYV G  IG GKF
Sbjct: 61  FKRRIAGVATAPPCGASSLVSSGRGLKRKIGCIDVSTQTGRKNKIDDDYVFGRNIGKGKF 120

Query: 121 GSVWLCKCKLTGAEYACKTLKKGEETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVM 180
           GSV +CK +  G E+ACKTLKKGEETVH+EVEIMQHLSG+P VVTL AVYEES+CFHLVM
Sbjct: 121 GSVRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVVTLHAVYEESDCFHLVM 180

Query: 181 ELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKI 240
           ELC GGRL+DQMV  G+YSE RAANI K++MLVI YCH+MGVVHRDIKPENILLT +GKI
Sbjct: 181 ELCSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKI 240

Query: 241 KLADFGLATRISCGQSLTGLAGSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQ 300
           +LADFGLA RI+ GQ+L+GLAGSPAYVAPEVLS NYSEKVD+WSAGVLL+ALL   LPF+
Sbjct: 241 QLADFGLAMRIAKGQTLSGLAGSPAYVAPEVLSENYSEKVDVWSAGVLLYALLSGVLPFK 300

Query: 301 GDSVESVFEAIKNTKLDFHSGMWESISKPARDLIEGMLTRDISARITAEEVLRHPWILFY 360
           GDS++++FEAIKN KLDF++G+WES+SKPARDL+  MLTR+ SARITA+EVLRHPWILFY
Sbjct: 301 GDSLDAIFEAIKNVKLDFNTGVWESVSKPARDLLARMLTREESARITADEVLRHPWILFY 360

Query: 361 TERTFKTLPVKLKLKNQ---ESCQQSPIAAKIKPNRNRFDSAAIAASLSEVSNLSSSENS 420
           T+RT KT+ +K K K+Q    +C Q+    + K + NR D      S S   + S++E  
Sbjct: 361 TDRTLKTMCIKSKHKSQAGSSTCLQNRSPTE-KTDLNRADREKKIPSDSPTDSFSNTE-- 420

Query: 421 NANSDDVDDSFLVDALATAISHVTISEPKRSRLCVPTGR-IEQHSSSNM-KANNLCKAF 475
               ++ D+S +VD L  AI++V ISEPKRSR+C PT   IEQ  SSN+   + LC+AF
Sbjct: 421 ----EEEDESGVVDVLVVAIANVRISEPKRSRVCSPTNNPIEQQHSSNLTSTSTLCRAF 470

BLAST of CmaCh01G016310 vs. TAIR 10
Match: AT1G12580.1 (phosphoenolpyruvate carboxylase-related kinase 1 )

HSP 1 Score: 260.0 bits (663), Expect = 3.7e-69
Identity = 127/264 (48.11%), Postives = 177/264 (67.05%), Query Frame = 0

Query: 104 NIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTLKKGE-------ETVHKEVEIMQH 163
           N+++ YV GE +G G+FG + +C  KLTG   ACK++ K         +++  E+ IM  
Sbjct: 39  NLKDRYVLGEQLGWGQFGVIRVCSDKLTGERLACKSISKDRLVTQDDMKSIKLEIAIMAK 98

Query: 164 LSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKY 223
           L+G+P+VV L AVYEE +  HLVMELC GG L  ++   G+YSE RA  + K +M V+K+
Sbjct: 99  LAGHPNVVNLKAVYEEKDSVHLVMELCAGGELFHKLEKYGRYSEVRARVLFKHLMQVVKF 158

Query: 224 CHDMGVVHRDIKPENILLTT---SGKIKLADFGLATRISCGQSLTGLAGSPAYVAPEVLS 283
           CHD G+VHRD+KPENIL+ T   S  IKLADFGLAT I  G+ L+G  GSP Y+APEVL+
Sbjct: 159 CHDSGIVHRDLKPENILMATMSSSSPIKLADFGLATYIKPGEKLSGTVGSPFYIAPEVLA 218

Query: 284 GNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSGMWESISKPARDL 343
           G Y++  D+WSAGV+L+ LL    PF G +   +F+A++   L F +  W++I+  A+DL
Sbjct: 219 GGYNQAADVWSAGVILYILLSGAPPFWGKTKSKIFDAVRAADLRFSAEPWDNITSYAKDL 278

Query: 344 IEGMLTRDISARITAEEVLRHPWI 358
           I GML  D S R++A+EVL H W+
Sbjct: 279 IRGMLCVDPSQRLSADEVLAHSWM 302

BLAST of CmaCh01G016310 vs. TAIR 10
Match: AT2G35890.1 (calcium-dependent protein kinase 25 )

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-65
Identity = 116/264 (43.94%), Postives = 174/264 (65.91%), Query Frame = 0

Query: 104 NIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTLKKGE-------ETVHKEVEIMQH 163
           +++  Y  G  +GHG+FG+ ++C  K TG EYACK++ K +       E V +E+EIM+H
Sbjct: 127 HLKEYYNLGSKLGHGQFGTTFVCVEKGTGEEYACKSIPKRKLENEEDVEDVRREIEIMKH 186

Query: 164 LSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKY 223
           L G P+V+++   YE+S   H+VMELCRGG L D++V  G YSE +AA++ K ++ V++ 
Sbjct: 187 LLGQPNVISIKGAYEDSVAVHMVMELCRGGELFDRIVERGHYSERKAAHLAKVILGVVQT 246

Query: 224 CHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISCGQSLTGLAGSPAYVAPEVLS 283
           CH +GV+HRD+KPEN L     +   +K  DFGL+  +  G++ T + GSP Y+APEVL+
Sbjct: 247 CHSLGVMHRDLKPENFLFVNDDEDSPLKAIDFGLSMFLKPGENFTDVVGSPYYIAPEVLN 306

Query: 284 GNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSGMWESISKPARDL 343
            NY  + DIWSAGV+++ LL  + PF G++ E +F  +   +LD  S  W  +S+ A+DL
Sbjct: 307 KNYGPEADIWSAGVMIYVLLSGSAPFWGETEEEIFNEVLEGELDLTSDPWPQVSESAKDL 366

Query: 344 IEGMLTRDISARITAEEVLRHPWI 358
           I  ML R+   R+TA++VL HPWI
Sbjct: 367 IRKMLERNPIQRLTAQQVLCHPWI 390

BLAST of CmaCh01G016310 vs. TAIR 10
Match: AT2G38910.1 (calcium-dependent protein kinase 20 )

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-64
Identity = 115/275 (41.82%), Postives = 177/275 (64.36%), Query Frame = 0

Query: 93  IDVATQIGRK-RNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTLKKGE------- 152
           + + + +GRK  N+++ Y  G  +G G+FG+ +LC  K TG E+ACKT+ K +       
Sbjct: 117 LQIDSVLGRKTENLKDIYSVGRKLGQGQFGTTFLCVDKKTGKEFACKTIAKRKLTTPEDV 176

Query: 153 ETVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAA 212
           E V +E++IM HLSG+P+V+ ++  YE++   H+VME+C GG L D+++  G Y+E +AA
Sbjct: 177 EDVRREIQIMHHLSGHPNVIQIVGAYEDAVAVHVVMEICAGGELFDRIIQRGHYTEKKAA 236

Query: 213 NILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISCGQSLTGLA 272
            + + ++ VI+ CH +GV+HRD+KPEN L  +  +   +K  DFGL+     G++ T + 
Sbjct: 237 ELARIIVGVIEACHSLGVMHRDLKPENFLFVSGDEEAALKTIDFGLSVFFKPGETFTDVV 296

Query: 273 GSPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSG 332
           GSP YVAPEVL  +YS + D+WSAGV+++ LL    PF  ++ + +FE +    LDF S 
Sbjct: 297 GSPYYVAPEVLRKHYSHECDVWSAGVIIYILLSGVPPFWDETEQGIFEQVLKGDLDFISE 356

Query: 333 MWESISKPARDLIEGMLTRDISARITAEEVLRHPW 357
            W S+S+ A+DL+  ML RD   R+T  EVL HPW
Sbjct: 357 PWPSVSESAKDLVRRMLIRDPKKRMTTHEVLCHPW 391

BLAST of CmaCh01G016310 vs. TAIR 10
Match: AT4G04720.1 (calcium-dependent protein kinase 21 )

HSP 1 Score: 243.4 bits (620), Expect = 3.6e-64
Identity = 120/275 (43.64%), Postives = 179/275 (65.09%), Query Frame = 0

Query: 94  DVATQIGRK-RNIENDYVSGETIGHGKFGSVWLCKCKLTGAEYACKTL-------KKGEE 153
           D  T +G+   +I   Y  G+ +G G+FG  ++CK   TG  YACK++       K+ +E
Sbjct: 64  DPDTILGKPFEDIRKFYSLGKELGRGQFGITYMCKEIGTGNTYACKSILKRKLISKQDKE 123

Query: 154 TVHKEVEIMQHLSGYPSVVTLLAVYEESECFHLVMELCRGGRLVDQMVSEGQYSEHRAAN 213
            V +E++IMQ+LSG P++V +   YE+ +  HLVMELC GG L D+++++G YSE  AA 
Sbjct: 124 DVKREIQIMQYLSGQPNIVEIKGAYEDRQSIHLVMELCAGGELFDRIIAQGHYSERAAAG 183

Query: 214 ILKEVMLVIKYCHDMGVVHRDIKPENILLTT---SGKIKLADFGLATRISCGQSLTGLAG 273
           I++ ++ V++ CH MGVVHRD+KPEN LL++   +  +K  DFGL+  I  G+    + G
Sbjct: 184 IIRSIVNVVQICHFMGVVHRDLKPENFLLSSKEENAMLKATDFGLSVFIEEGKVYRDIVG 243

Query: 274 SPAYVAPEVLSGNYSEKVDIWSAGVLLHALLVATLPFQGDSVESVFEAIKNTKLDFHSGM 333
           S  YVAPEVL  +Y +++DIWSAGV+L+ LL    PF  ++ + +F+ +   ++DF S  
Sbjct: 244 SAYYVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKGEIDFVSEP 303

Query: 334 WESISKPARDLIEGMLTRDISARITAEEVLRHPWI 358
           W SIS+ A+DL+  MLT+D   RITA +VL HPWI
Sbjct: 304 WPSISESAKDLVRKMLTKDPKRRITAAQVLEHPWI 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4901.6e-16263.05Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2... [more]
Q6F3A61.4e-6541.32Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q9SJ611.6e-6443.94Calcium-dependent protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CPK25 PE=... [more]
Q9ZV152.3e-6341.82Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=... [more]
P536843.9e-6344.44Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1ISH92.7e-269100.00serine/threonine-protein kinase PEPKR2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1FL123.1e-26598.52serine/threonine-protein kinase PEPKR2-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A5D3E5W27.5e-23588.82Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CQM37.5e-23588.82serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 P... [more]
A0A6J1IGJ41.3e-23488.61serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC1114767... [more]
Match NameE-valueIdentityDescription
XP_022980892.15.6e-269100.00serine/threonine-protein kinase PEPKR2-like [Cucurbita maxima][more]
KAG7037610.14.4e-26698.95Serine/threonine-protein kinase PEPKR2 [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022940947.16.4e-26598.52serine/threonine-protein kinase PEPKR2-like [Cucurbita moschata][more]
XP_023524190.11.7e-26297.70serine/threonine-protein kinase PEPKR2-like [Cucurbita pepo subsp. pepo][more]
KAG6608267.11.0e-25498.90Serine/threonine-protein kinase PEPKR2, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
AT1G12680.11.2e-16363.05phosphoenolpyruvate carboxylase-related kinase 2 [more]
AT1G12580.13.7e-6948.11phosphoenolpyruvate carboxylase-related kinase 1 [more]
AT2G35890.11.1e-6543.94calcium-dependent protein kinase 25 [more]
AT2G38910.11.6e-6441.82calcium-dependent protein kinase 20 [more]
AT4G04720.13.6e-6443.64calcium-dependent protein kinase 21 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 109..357
e-value: 4.1E-92
score: 322.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 109..357
e-value: 3.6E-69
score: 233.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 109..357
score: 48.66832
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 101..426
e-value: 9.6E-86
score: 290.0
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 145..311
e-value: 3.4E-9
score: 33.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 26..462
NoneNo IPR availablePANTHERPTHR24349:SF261SERINE/THREONINE-PROTEIN KINASE PEPKR2coord: 26..462
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 108..356
e-value: 3.44505E-114
score: 334.83
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 222..234
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 115..138
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 101..376

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G016310.1CmaCh01G016310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity