CmaCh01G015940 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G015940
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionEngB-type G domain-containing protein
LocationCma_Chr01: 11010643 .. 11015389 (-)
RNA-Seq ExpressionCmaCh01G015940
SyntenyCmaCh01G015940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACCAGAGCACACCGCTATTCCTTCCTCAAAAATCTCCAATGAGAGCCGAGCAAGTTCTCATAGGCATAGAGATGGTTCTCTCTCAGCTCTCTAAACTCAACCGTTCTCTCTTTACTTCACCAATCCGCCGAAGTTTTAGCGTATTTGCAAAAACCAATAAACCCATAATCGCGGCCACAGAAGCCTCCATTCTCACTTCGCCTTCATTTTCCGCCCCCAAATCCACTCTCACGGCCGTCGAACCAGTTTCGATCACCGATTTTTCAGAATATCCCGAAGAACAACAGTTAACCATCTCCCCTGAGAAGCTATTTATTCCGCCGGAAACTGATTTCTCCGGCAGCGGCGCTGCGGGGACGAGAATCTTGAAGGGTTCGAACATTGTGTTGGGACGGTACGCGAGGGATGCGCAGATTTCCGAGGCGAATTTTGTGAAGAGTAGTGTGAGAACCGAGGACTGCCCTTCTGATGGGCTTCCTGAATTTGCCCTTGTTGGGCGCTCGAATGTGGGGAAATCGTCACTGTTGAACTCGCTCGTTCGCCGGAAGAAGCTCGCTCATACCTCCAAGAAACCAGGTCGACGGTTTGTTTTGCCATATTGTTTCTTTAATTAGTTGTGTTATTTTCATTTGTTGTGAAGCCACTGCTTTGAAAGGAAAAATGGTGTAGTTTCGTAGTCCATGAGAACTCTCCTAGCACTTGTGATATGTTTGGTGTAGTTTCATATTGTCCCTAATGCTTAACGATATTGTCCGTTTTAGCTCGTTACGTATCGCCGTCAGCCTCACGCATTTGCTAGGGAGAGGTTTGCACACTCTTATAAAGAATGTTTCGTTCCACTCTCTAACCGGTATCTCACAATCCACCTCCTTTAGGGCCCAACGTCCTCGCTAATTCACTGCTCAGTGGCTGGTTTTGATACCATTTGTAACAACCCAAGTCCACCGCCGGTAAATATTGTCTGCTTTAATCCATTACGTATCATCGTTAACCTTTTGAAACGCGTCTGTTAGGGAGAAGTTTCCACACCTTTATAAGGAATGCTTCGTTCCCCTCTTCAACCGAGGTGGGATCTCAGATAATAGATAATAGGAACTAGAGGACTGTACATGATTTTGAAGAAAATTGAACAACATAGCTTGAACAGAACAAATATCTGCCAAACTTCTTTGAACTATGCGTTTCATTGATTCAAAGCCTTGACCTTATAACTCCATTTTCTCATCATTCATCAAGGCTCTGTTGTTATGAATGATTGGCGTGTTAAATCCCATCCAATCTACGCATGCAAGTGTAGGGGTATATTAAGATTTTGAAGCTTTCATCCAATTTGCCTTCTTAAGCTAATAGAAGTGAAACATTATCGATAAAATGGGGTCGAGCTCAATCAAAACTGCCACTACTCCCAAGTGGGGTTCATTGGCACTCTCACTTCCCGACATAGATACGAGCATGTTGAGCTTTTCAGTGAGAATGAAAGTGTAAATGAATCGACCTCAAATAGAGGAATTCCTGGTCCTGTTTATATTCTTGCATTAACGGGAATGTAAAATTTAGATCCTTCTCGTGAAATTTTCTATCTCCGCTACTTGCCAGCACGTGAGAGAGAAAGGGCAAGAGAAGGAATAAGCTAATTAATGAGTTATCATAGGCCTTTTCTGTTATGATTCGAATGTCTGAAATCCCTTGTACTGTTTAATCGCAGATGGTCTCTTCCAATTGTTCTATTAAGAATGTTGGATGTGGATATAAACACTTTGTGTTTTATTGCAGGAAAGACACAGTGCATAAACCATTTTCGAATCAACGATAGTTGGTACTTGGTCGATTTGCCTGGATATGGGTATGTCTTAACTTCGAGTTGTGTCGTTGTCAGATCCCTTTTCAAAGTTCGAACTTGCTTCCATGTGACTTGCTCTGTGATGGGGCATGAGTTTGCCTCTGCCCTTATTCCTTCATATGTGCTACCTCTATTTGAGTAAATGCCAATGGCATTTGTTTGTTCCTTTTTTGTAGGTATGCATCTGCACCACGGGAACTCCGAACGGACTGGAATAAGTTTACCAAGGACTATTTCCTGAATCGGTCAACGTTGGTTTCGGTTTTCCTTCTTATAGATGCCAGCGTTCCTGCCAAACAAATTGATCTTGACTATGCTAGTTGGTTGGGCCAGAATCAGGTAATATTCTTCTTGCATGTATGAGGCATATAGAAGGAAAATATCCAATTTGCATATCTTATTTTTCTTAAAAAGACAATATCTTTTAAGAAGCTGAAAGTATTGAAGGCTGTGAGAGTACAAGAGTATGCATATGATATACTATGGGAGATCCCATGTTGGTTGGAGAAGGGAACGAAACATTCTTTATAAGGGTGTAGAAACCTCTCCCTAACAGACGTGATTTAAAACCTTGAGGGGAAACCCAGAAGAGAAAGTCCAAATAGGACAATGTCTGCTAACGGTGGGCTTGGCTGTTACAAATGGTATCAAAGTTAGGCATCGGGCGGTGTGTCAGCGGAAACGCTGGGCCCTCAAGGGGGATGAATTATGAAATCCCACATTAGTTGGAGAGGGAAACAAAGCATTCTTTATAAGGGTGTAGAAACCTCTCTATAACAGACGCGTTTTAAATTCTTGAGGGAAGCCTAAAAAAAAAGCTCACTGAGAACCATATCTGCTAATGGTGGGCTTGAGCTGATACAAATGGTATCAAAGCCAGGCACGGGCCGATGTGCCAGCGGGGACACAGGGCCTCCAAGAGGAGTGGATTGTGAGATCCCACATTGGTTGGAGAGAGAAACAAAGTATTCTTTATAAGGGTGTGGAAACCTCTCTCTACCAGAAACGTTTTAAAACTTTGAGGGAAAGTTTAAAAGGGAAAGTCCAAGAAGGACAATATCCACTAGCGGTGAGCTTGAGCTGTTACATGCTATGGGTTAAGTGTGGGGATCAATAGCGTATTAGTTAAGTGTGGGGATCAATAGCGTATTAGTTAAGGACAAAGTTGTAATTAGTTAGGGGTATCTTGGTTATAAATAAGAGTGTTGGTCCTCTCGAAAGGCTATTGTTACATAGAGATATACTACTATCTTGTGTTTTTGGTGTTGGCTGCCTATAAAAAACCACCAAAAATAACAAAACAAGGGTTGCAAAGATTCAAGAACAAAGACTCGCAGATTCTTGCTAGAGGCATTGAAGTATCCATTCCTGCATACCCCCTGTATTAAAAACAAGAGATCTTCGTTTCTTAATTTGTATTGTAAGAGCCAAATCGAAAGTCTAAGAATCGGGTTTATTTACTAGAAACTTTGTGTGAGATCCTACCTTGGTTGGAGGGGAGAGTAAAGCATTCTTTATAAGAGTGTGAAAACCTCTCCCTAACAGACACGTTTTAAAACTGTGAGGCTGACAGTGATATGTAACACGCCAAAGCGGACAATATCTGCTAGCGGTAGGTTCGGACTGTTGCAAATGGTATCAGAGCTAGACACCGAACGATGTGCTTCTAAGGGAGGTGGATTGTGAGATCCCACGTCGGTTGAAGAAGATAACGAAGCATAACTTATAAGGATGTGGAAACCTCTCTCTAACAGACGTATTTTTAAACTGTGAGACTGACGGCGATACATCACGGGCCAAAGTGAACAATATCTGCTAGTAGTGGGTTTGGACTGTTATATTTTATTTCCCATGATTTTCAGCCTTCGCCCTTCACTTAACTTGTTCCAAATCTTCTTCTGAACCTTTAGGAACAAGAATCAGCCCCTAAGTCTAATATTGTACGGTAGAAATTCATTCAATTTGATGCATTCGTTTTCAAGAAAATTCGACATGGAATGTTTCCTGCAGATTCCAATGACGATAATATTTACGAAATGCGACAAACGAAAGAAGAAAAAGAAAGATGGAAAAAGAGCAGAAGAAAATATGAGCGACTTTCAGGAACTGATTGGTGGATTTTTCCACACGACTCCGCCGTGGATTATGACCAGCAGTGTCACCCATCAAGGTCGGGACGAAATTCTTCTGCATATCGCTCAACTGAGGAACTACTGGCTCAAGCACTGATAAAACACTTCAGCCCGCCTTCCCATGTCGATCAGGTACGACCTCATATTGGTTATGCTCATGCTTGCTATATATAGAGATATCCGCTTCTTGTCCAAAAGTAGGTTCTCTCGATTTCGAGCTCCAAAATTAACAATGGCTGCAAATGTTGCAGCTTGCTGATGTTTCTCGACACGAATAGATGCACTTCATCTTGACTCAAAACGGGTCGATAGAATGTGATTCCTGTATTATATATTCAACAGCTCGAGGTCGGACGAGGACACAAAGTTTGGAGAGTTTACAAACCAACACTAACACGGCTTAATAGTGTTTCACAAGCTTCATTCTGTCACTGCTTAAAAGCTTCAAACAGGGCAATATAATGGAAGATTAGTAGAGAGTTGCTCTATTATCTTGTGGATCCCGTTTATTCCTGTTCTGGATAAGGATGGGTTTACTCCTTTGCCCAAACACTCGGCTTCATTACTCGGAGACTTGGCCTTGTTGCAAACCTCATAACTGCTGGCTTGTCCTTCTCTTTTTCCTTTAAAATAATATCCTTTAACAGTAAATTTTGGGTGTAAACAATTTCTTGGCTGTTTAACTTTTTACCATTATTATGCTTTAGTTTGTACTCTAACTTGATGCCAAGTATCGAGATTTAAAAGTTTACCACAGTAAAATTTAAAGATTTA

mRNA sequence

GACCAGAGCACACCGCTATTCCTTCCTCAAAAATCTCCAATGAGAGCCGAGCAAGTTCTCATAGGCATAGAGATGGTTCTCTCTCAGCTCTCTAAACTCAACCGTTCTCTCTTTACTTCACCAATCCGCCGAAGTTTTAGCGTATTTGCAAAAACCAATAAACCCATAATCGCGGCCACAGAAGCCTCCATTCTCACTTCGCCTTCATTTTCCGCCCCCAAATCCACTCTCACGGCCGTCGAACCAGTTTCGATCACCGATTTTTCAGAATATCCCGAAGAACAACAGTTAACCATCTCCCCTGAGAAGCTATTTATTCCGCCGGAAACTGATTTCTCCGGCAGCGGCGCTGCGGGGACGAGAATCTTGAAGGGTTCGAACATTGTGTTGGGACGGTACGCGAGGGATGCGCAGATTTCCGAGGCGAATTTTGTGAAGAGTAGTGTGAGAACCGAGGACTGCCCTTCTGATGGGCTTCCTGAATTTGCCCTTGTTGGGCGCTCGAATGTGGGGAAATCGTCACTGTTGAACTCGCTCGTTCGCCGGAAGAAGCTCGCTCATACCTCCAAGAAACCAGGAAAGACACAGTGCATAAACCATTTTCGAATCAACGATAGTTGGTACTTGGTCGATTTGCCTGGATATGGGTATGCATCTGCACCACGGGAACTCCGAACGGACTGGAATAAGTTTACCAAGGACTATTTCCTGAATCGGTCAACGTTGGTTTCGGTTTTCCTTCTTATAGATGCCAGCGTTCCTGCCAAACAAATTGATCTTGACTATGCTAGTTGGTTGGGCCAGAATCAGATTCCAATGACGATAATATTTACGAAATGCGACAAACGAAAGAAGAAAAAGAAAGATGGAAAAAGAGCAGAAGAAAATATGAGCGACTTTCAGGAACTGATTGGTGGATTTTTCCACACGACTCCGCCGTGGATTATGACCAGCAGTGTCACCCATCAAGGTCGGGACGAAATTCTTCTGCATATCGCTCAACTGAGGAACTACTGGCTCAAGCACTGATAAAACACTTCAGCCCGCCTTCCCATGTCGATCAGCTTGCTGATGTTTCTCGACACGAATAGATGCACTTCATCTTGACTCAAAACGGGTCGATAGAATGTGATTCCTGTATTATATATTCAACAGCTCGAGGTCGGACGAGGACACAAAGTTTGGAGAGTTTACAAACCAACACTAACACGGCTTAATAGTGTTTCACAAGCTTCATTCTGTCACTGCTTAAAAGCTTCAAACAGGGCAATATAATGGAAGATTAGTAGAGAGTTGCTCTATTATCTTGTGGATCCCGTTTATTCCTGTTCTGGATAAGGATGGGTTTACTCCTTTGCCCAAACACTCGGCTTCATTACTCGGAGACTTGGCCTTGTTGCAAACCTCATAACTGCTGGCTTGTCCTTCTCTTTTTCCTTTAAAATAATATCCTTTAACAGTAAATTTTGGGTGTAAACAATTTCTTGGCTGTTTAACTTTTTACCATTATTATGCTTTAGTTTGTACTCTAACTTGATGCCAAGTATCGAGATTTAAAAGTTTACCACAGTAAAATTTAAAGATTTA

Coding sequence (CDS)

ATGAGAGCCGAGCAAGTTCTCATAGGCATAGAGATGGTTCTCTCTCAGCTCTCTAAACTCAACCGTTCTCTCTTTACTTCACCAATCCGCCGAAGTTTTAGCGTATTTGCAAAAACCAATAAACCCATAATCGCGGCCACAGAAGCCTCCATTCTCACTTCGCCTTCATTTTCCGCCCCCAAATCCACTCTCACGGCCGTCGAACCAGTTTCGATCACCGATTTTTCAGAATATCCCGAAGAACAACAGTTAACCATCTCCCCTGAGAAGCTATTTATTCCGCCGGAAACTGATTTCTCCGGCAGCGGCGCTGCGGGGACGAGAATCTTGAAGGGTTCGAACATTGTGTTGGGACGGTACGCGAGGGATGCGCAGATTTCCGAGGCGAATTTTGTGAAGAGTAGTGTGAGAACCGAGGACTGCCCTTCTGATGGGCTTCCTGAATTTGCCCTTGTTGGGCGCTCGAATGTGGGGAAATCGTCACTGTTGAACTCGCTCGTTCGCCGGAAGAAGCTCGCTCATACCTCCAAGAAACCAGGAAAGACACAGTGCATAAACCATTTTCGAATCAACGATAGTTGGTACTTGGTCGATTTGCCTGGATATGGGTATGCATCTGCACCACGGGAACTCCGAACGGACTGGAATAAGTTTACCAAGGACTATTTCCTGAATCGGTCAACGTTGGTTTCGGTTTTCCTTCTTATAGATGCCAGCGTTCCTGCCAAACAAATTGATCTTGACTATGCTAGTTGGTTGGGCCAGAATCAGATTCCAATGACGATAATATTTACGAAATGCGACAAACGAAAGAAGAAAAAGAAAGATGGAAAAAGAGCAGAAGAAAATATGAGCGACTTTCAGGAACTGATTGGTGGATTTTTCCACACGACTCCGCCGTGGATTATGACCAGCAGTGTCACCCATCAAGGTCGGGACGAAATTCTTCTGCATATCGCTCAACTGAGGAACTACTGGCTCAAGCACTGA

Protein sequence

MRAEQVLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQGRDEILLHIAQLRNYWLKH
Homology
BLAST of CmaCh01G015940 vs. ExPASy Swiss-Prot
Match: O81004 (GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV=2)

HSP 1 Score: 297.4 bits (760), Expect = 2.1e-79
Identity = 149/277 (53.79%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 50  SILTSPSFSAPKSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRI 109
           + ++ P  SA  +  T     SI+D +++        +   LFIPP  +          +
Sbjct: 34  NFISIPKISASTNNPTTTTNRSISDATKF--------AKSVLFIPPGVEI--EELTDDMV 93

Query: 110 LKGSNIVLGRYARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRR 169
           L GSNIV+G +A  +QI E  FVKSS R  DCP D  PE A++GRSNVGKSSL+N LVR+
Sbjct: 94  LPGSNIVIGPFAGHSQIKEVEFVKSSARARDCPKDDRPEIAILGRSNVGKSSLINCLVRK 153

Query: 170 KKLAHTSKKPGKTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTL 229
           K++A TSKKPGKTQ INHF +N SWY+VDLPGYG+A      +TDW+ FTK YFLNR +L
Sbjct: 154 KEVALTSKKPGKTQLINHFLVNKSWYIVDLPGYGFAKVSDAAKTDWSAFTKGYFLNRDSL 213

Query: 230 VSVFLLIDASVPAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQE 289
           V V LLIDASVP ++IDLD A+WLG+N +PMT +FTKCDK K  K  GKR +EN+  FQ+
Sbjct: 214 VCVLLLIDASVPPQKIDLDCANWLGRNNVPMTFVFTKCDKMKATK--GKRPDENIKAFQQ 273

Query: 290 LIGGFFHTTPPWIMTSSVTHQGRDEILLHIAQLRNYW 327
           +I   F   PPWI+TSSV+  GRDE+LLH++QLRNYW
Sbjct: 274 IIRENFKVHPPWILTSSVSGLGRDELLLHMSQLRNYW 298

BLAST of CmaCh01G015940 vs. ExPASy Swiss-Prot
Match: A5FIZ1 (Probable GTP-binding protein EngB OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=engB PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.3e-45
Identity = 90/205 (43.90%), Postives = 131/205 (63.90%), Query Frame = 0

Query: 125 QISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQC 184
           +I+ A F+ S+     CP D LPE+A +GRSNVGKSSL+N +   K LA TS KPGKTQ 
Sbjct: 2   KINTAEFIVSNSDAAKCPKDFLPEYAFIGRSNVGKSSLINMITNHKNLAKTSGKPGKTQL 61

Query: 185 INHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQ 244
           INHF+IN++W+LVDLPGYGYA   ++ ++ + +F  DYF NR  LV  F+LID    A++
Sbjct: 62  INHFKINNNWFLVDLPGYGYAKVSKKTKSIFQQFITDYFENREQLVCAFVLIDIRHEAQK 121

Query: 245 IDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMT 304
           ID+++ S++G+++IP  IIFTK DK  K K D   A       +++    +   P + +T
Sbjct: 122 IDIEFMSYMGESEIPFCIIFTKADKISKGKIDSHIAAYR----KQMYANNWAEMPQYFVT 181

Query: 305 SSVTHQGRDEILLHIAQLRNYWLKH 330
           SS    G+DE+L +I ++     K+
Sbjct: 182 SSTESIGKDELLTYIDEVNQEVFKN 202

BLAST of CmaCh01G015940 vs. ExPASy Swiss-Prot
Match: A6L4J3 (Probable GTP-binding protein EngB OS=Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154) OX=435590 GN=engB PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 89/195 (45.64%), Postives = 123/195 (63.08%), Query Frame = 0

Query: 125 QISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQC 184
           +I  A FV S+ + E CP D LPE+A +GRSNVGKSSL+N L +R KLA TS  PGKT  
Sbjct: 2   EILSAEFVVSNTKVEKCPQDNLPEYAFIGRSNVGKSSLINMLAKRPKLAMTSSTPGKTLL 61

Query: 185 INHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQ 244
           INHF IN  WYLVDLPGYGYAS  ++      +  +DY L R  + ++F+LID  +  ++
Sbjct: 62  INHFLINKEWYLVDLPGYGYASRGKKQVEKIQQIIEDYILEREQMTNLFVLIDCRLEPQK 121

Query: 245 IDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMT 304
           IDL++  WLG+N +P +IIFTK DK    K      ++N++ + + +   +   PP  ++
Sbjct: 122 IDLEFMEWLGENGVPFSIIFTKADKLTNGK-----VKDNVNKYLKKLTEQWEELPPHFVS 181

Query: 305 SSVTHQGRDEILLHI 320
           SS    GR EIL +I
Sbjct: 182 SSEKKTGRQEILDYI 191

BLAST of CmaCh01G015940 vs. ExPASy Swiss-Prot
Match: Q8A8T4 (Probable GTP-binding protein EngB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=engB PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.1e-44
Identity = 89/198 (44.95%), Postives = 125/198 (63.13%), Query Frame = 0

Query: 125 QISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQC 184
           +I+ A FV S+   + CP+   PE+A +GRSNVGKSSL+N L  RK LA TS  PGKT  
Sbjct: 2   EITSAEFVISNTDVKKCPTGVFPEYAFIGRSNVGKSSLINMLTARKGLAMTSATPGKTML 61

Query: 185 INHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQ 244
           INHF IN SWYLVDLPGYGYA   ++ +       +DY L R  + ++F+LID+ +  ++
Sbjct: 62  INHFLINQSWYLVDLPGYGYARRGQKGKDQIRTIIEDYILEREQMTNLFVLIDSRLEPQK 121

Query: 245 IDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMT 304
           IDL++  WLG+N IP +IIFTK DK K     G R + N++++   +   +   PP+ ++
Sbjct: 122 IDLEFMEWLGENGIPFSIIFTKADKLK-----GGRLKMNINNYLRELSKEWEELPPYFIS 181

Query: 305 SSVTHQGRDEILLHIAQL 323
           SS    GR EIL +I  +
Sbjct: 182 SSENRTGRTEILDYIENI 194

BLAST of CmaCh01G015940 vs. ExPASy Swiss-Prot
Match: Q5LDL2 (Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=engB PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.7e-44
Identity = 90/198 (45.45%), Postives = 124/198 (62.63%), Query Frame = 0

Query: 125 QISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQC 184
           +I+ A FV S+   + CP+   PE+A +GRSNVGKSSL+N L  RK LA TS  PGKT  
Sbjct: 2   EITNAEFVISNTDVKKCPAGTFPEYAFIGRSNVGKSSLINMLTGRKGLAMTSATPGKTML 61

Query: 185 INHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQ 244
           INHF IN+SWYLVDLPGYGYA   ++ +       +DY L R  + ++F+LID+ +  ++
Sbjct: 62  INHFLINNSWYLVDLPGYGYARRGQKGQKQIRTIIEDYILEREQMTNLFVLIDSRLEPQK 121

Query: 245 IDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMT 304
           IDL++  WLG+N IP  IIFTK DK K     G R + N+S +   +   +   PP+ +T
Sbjct: 122 IDLEFMEWLGENGIPFAIIFTKADKLK-----GGRLKINISAYLRELRKQWEELPPYFIT 181

Query: 305 SSVTHQGRDEILLHIAQL 323
           SS    GR E+L +I  +
Sbjct: 182 SSEERLGRTEVLNYIESI 194

BLAST of CmaCh01G015940 vs. ExPASy TrEMBL
Match: A0A6J1J1E8 (GTP-binding protein At2g22870-like OS=Cucurbita maxima OX=3661 GN=LOC111480403 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MRAEQVLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAP 60
           MRAEQVLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAP
Sbjct: 1   MRAEQVLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAP 60

Query: 61  KSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRY 120
           KSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRY
Sbjct: 61  KSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRY 120

Query: 121 ARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPG 180
           ARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPG
Sbjct: 121 ARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPG 180

Query: 181 KTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASV 240
           KTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASV
Sbjct: 181 KTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASV 240

Query: 241 PAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPP 300
           PAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPP
Sbjct: 241 PAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPP 300

Query: 301 WIMTSSVTHQGRDEILLHIAQLRNYWLKH 330
           WIMTSSVTHQGRDEILLHIAQLRNYWLKH
Sbjct: 301 WIMTSSVTHQGRDEILLHIAQLRNYWLKH 329

BLAST of CmaCh01G015940 vs. ExPASy TrEMBL
Match: A0A6J1FJT1 (GTP-binding protein At2g22870-like OS=Cucurbita moschata OX=3662 GN=LOC111445985 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.4e-176
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0

Query: 12  MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVS 71
           MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKP+IAATEASILTSPSF APKSTLTA EPVS
Sbjct: 1   MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPVIAATEASILTSPSFPAPKSTLTAAEPVS 60

Query: 72  ITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 131
           ITDFSE+PEE+QLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF
Sbjct: 61  ITDFSEHPEEKQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 120

Query: 132 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 191
           VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN
Sbjct: 121 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 180

Query: 192 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 251
           DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYA 
Sbjct: 181 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAG 240

Query: 252 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 311
           WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG
Sbjct: 241 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 300

Query: 312 RDEILLHIAQLRNYWLKH 330
           RDEILLHIAQLRNYWLKH
Sbjct: 301 RDEILLHIAQLRNYWLKH 318

BLAST of CmaCh01G015940 vs. ExPASy TrEMBL
Match: A0A0A0LGU2 (EngB-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G888580 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.3e-155
Identity = 275/318 (86.48%), Postives = 292/318 (91.82%), Query Frame = 0

Query: 12  MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVS 71
           MVLS LS++N  LFTSPI R+FS+FAKTNKPIIAAT A ILTS  F  PKSTLTA EP+ 
Sbjct: 1   MVLSHLSRINNFLFTSPIHRNFSLFAKTNKPIIAATGAPILTSTPFPVPKSTLTAAEPIP 60

Query: 72  ITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 131
           IT+F+EYPEE QL ISPEKLF+PP+TD SG G  G RILKGSNIVLGRYARDAQ+S+A+F
Sbjct: 61  ITEFTEYPEETQLPISPEKLFVPPDTDISGGG--GARILKGSNIVLGRYARDAQVSQADF 120

Query: 132 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 191
           VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLA TSKKPGKTQCINHFRIN
Sbjct: 121 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLALTSKKPGKTQCINHFRIN 180

Query: 192 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 251
           DSWYLVDLPGYGYA+AP+ELRTDWNKFT+DYFLNRSTLVSVFLLIDAS+PAK IDLDYAS
Sbjct: 181 DSWYLVDLPGYGYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYAS 240

Query: 252 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 311
           WLGQNQIPMTIIFTKCDKRKKKK  GKR EEN+SDFQELI GFF TTPPWIMTSSVTHQG
Sbjct: 241 WLGQNQIPMTIIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQG 300

Query: 312 RDEILLHIAQLRNYWLKH 330
           RDEILLHIAQLRNYWLKH
Sbjct: 301 RDEILLHIAQLRNYWLKH 316

BLAST of CmaCh01G015940 vs. ExPASy TrEMBL
Match: A0A5A7T5H0 (GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006340 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.0e-153
Identity = 274/318 (86.16%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 12  MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVS 71
           MVLS LS++N  LFTSPI R+FSVFAKTNKPII AT A ILTS S   PKSTLTA EP+ 
Sbjct: 1   MVLSHLSRINTFLFTSPIHRNFSVFAKTNKPIITATAAPILTSTSLPVPKSTLTAAEPIP 60

Query: 72  ITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 131
           IT+FSEY EE QL ISPEKLF+PPETD SG G  G RILKGSNIVLGRYARDAQ+S+A+F
Sbjct: 61  ITEFSEYQEETQLPISPEKLFVPPETDISGGG--GARILKGSNIVLGRYARDAQVSQADF 120

Query: 132 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 191
           VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLA TSKKPGKTQCINHFRIN
Sbjct: 121 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLALTSKKPGKTQCINHFRIN 180

Query: 192 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 251
           DSWYLVDLPGYGYA+AP+ELRTDWNKFT+DYFLNRSTLVSVFLLIDAS+PAK IDL+YAS
Sbjct: 181 DSWYLVDLPGYGYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLEYAS 240

Query: 252 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 311
           WLGQNQIPMTIIFTKCDKRKKKK  GKR EEN+SDFQELI GFF TTPPWIMTSSVTHQG
Sbjct: 241 WLGQNQIPMTIIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQG 300

Query: 312 RDEILLHIAQLRNYWLKH 330
           RDEILLH+AQLRNYWLKH
Sbjct: 301 RDEILLHMAQLRNYWLKH 316

BLAST of CmaCh01G015940 vs. ExPASy TrEMBL
Match: A0A6J1IKL5 (GTP-binding protein At2g22870-like OS=Cucurbita maxima OX=3661 GN=LOC111476474 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 275/324 (84.88%), Postives = 291/324 (89.81%), Query Frame = 0

Query: 6   VLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLT 65
           +L   EMVLSQ SK+N SLFT PI  +FS F   NKPIIAAT A I TS SF  PKST+T
Sbjct: 1   MLTNSEMVLSQFSKINISLFTLPIHHNFSAF-PANKPIIAATAAPIFTSTSFQLPKSTVT 60

Query: 66  AVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQ 125
             EP+ ITDFSEYPEE QL+ISP+KLF+PPETD SG GAAG RILKGSNIVLGRYARDAQ
Sbjct: 61  PAEPIPITDFSEYPEEIQLSISPDKLFVPPETDISGGGAAGPRILKGSNIVLGRYARDAQ 120

Query: 126 ISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCI 185
           +S+A+FVKSSV TEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLA TSKKPGKTQCI
Sbjct: 121 VSQADFVKSSVTTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLALTSKKPGKTQCI 180

Query: 186 NHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQI 245
           NHFRINDSWYLVDLPGYGYA+AP+ELRTDWNKFTK+YFLNRSTLVSVFLLIDAS+PAKQI
Sbjct: 181 NHFRINDSWYLVDLPGYGYAAAPQELRTDWNKFTKNYFLNRSTLVSVFLLIDASIPAKQI 240

Query: 246 DLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTS 305
           DLDYASWLGQNQIPMTIIFTKCDKRKKKK  GKR EEN+SDF ELI GFF TTPPWIMTS
Sbjct: 241 DLDYASWLGQNQIPMTIIFTKCDKRKKKKNGGKRPEENVSDFHELISGFFQTTPPWIMTS 300

Query: 306 SVTHQGRDEILLHIAQLRNYWLKH 330
           SVTHQGRDEILLHIAQLRNYWLKH
Sbjct: 301 SVTHQGRDEILLHIAQLRNYWLKH 323

BLAST of CmaCh01G015940 vs. NCBI nr
Match: XP_022981183.1 (GTP-binding protein At2g22870-like [Cucurbita maxima])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MRAEQVLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAP 60
           MRAEQVLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAP
Sbjct: 1   MRAEQVLIGIEMVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAP 60

Query: 61  KSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRY 120
           KSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRY
Sbjct: 61  KSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRY 120

Query: 121 ARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPG 180
           ARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPG
Sbjct: 121 ARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPG 180

Query: 181 KTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASV 240
           KTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASV
Sbjct: 181 KTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASV 240

Query: 241 PAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPP 300
           PAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPP
Sbjct: 241 PAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPP 300

Query: 301 WIMTSSVTHQGRDEILLHIAQLRNYWLKH 330
           WIMTSSVTHQGRDEILLHIAQLRNYWLKH
Sbjct: 301 WIMTSSVTHQGRDEILLHIAQLRNYWLKH 329

BLAST of CmaCh01G015940 vs. NCBI nr
Match: XP_022940339.1 (GTP-binding protein At2g22870-like [Cucurbita moschata])

HSP 1 Score: 627.9 bits (1618), Expect = 5.1e-176
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0

Query: 12  MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVS 71
           MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKP+IAATEASILTSPSF APKSTLTA EPVS
Sbjct: 1   MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPVIAATEASILTSPSFPAPKSTLTAAEPVS 60

Query: 72  ITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 131
           ITDFSE+PEE+QLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF
Sbjct: 61  ITDFSEHPEEKQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 120

Query: 132 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 191
           VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN
Sbjct: 121 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 180

Query: 192 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 251
           DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYA 
Sbjct: 181 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAG 240

Query: 252 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 311
           WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG
Sbjct: 241 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 300

Query: 312 RDEILLHIAQLRNYWLKH 330
           RDEILLHIAQLRNYWLKH
Sbjct: 301 RDEILLHIAQLRNYWLKH 318

BLAST of CmaCh01G015940 vs. NCBI nr
Match: XP_023524307.1 (GTP-binding protein At2g22870-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 311/318 (97.80%), Postives = 313/318 (98.43%), Query Frame = 0

Query: 12  MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVS 71
           MVLSQLS+LNRSLFTS IRRSFSVFAKTNKPIIAATEASILTSPSF APKS LTA EPVS
Sbjct: 1   MVLSQLSRLNRSLFTSSIRRSFSVFAKTNKPIIAATEASILTSPSFPAPKSILTAAEPVS 60

Query: 72  ITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 131
           ITDFSEYPEE+QLTISPEKLFIPPETD SGSGAAGTRILKGSNIVLGRYARDAQISEANF
Sbjct: 61  ITDFSEYPEEKQLTISPEKLFIPPETDLSGSGAAGTRILKGSNIVLGRYARDAQISEANF 120

Query: 132 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 191
           VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN
Sbjct: 121 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 180

Query: 192 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 251
           DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS
Sbjct: 181 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 240

Query: 252 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 311
           WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG
Sbjct: 241 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 300

Query: 312 RDEILLHIAQLRNYWLKH 330
           RDEILLHIAQLRNYWLKH
Sbjct: 301 RDEILLHIAQLRNYWLKH 318

BLAST of CmaCh01G015940 vs. NCBI nr
Match: KAG6608233.1 (GTP-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 308/318 (96.86%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 12  MVLSQLSKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVS 71
           MVLSQLSKLNR LFTSPIRRSFSVF KTNKPIIAATE SILTSPS  APKSTLTA EPVS
Sbjct: 1   MVLSQLSKLNRPLFTSPIRRSFSVFVKTNKPIIAATEPSILTSPSIPAPKSTLTAAEPVS 60

Query: 72  ITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANF 131
           ITDFSE+PEE+QLTI+PEKLFIPPETDFSGSG AGTRILKGSNIVLGRYARDAQISEANF
Sbjct: 61  ITDFSEHPEEKQLTIAPEKLFIPPETDFSGSGVAGTRILKGSNIVLGRYARDAQISEANF 120

Query: 132 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 191
           VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN
Sbjct: 121 VKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRIN 180

Query: 192 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 251
           DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS
Sbjct: 181 DSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYAS 240

Query: 252 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 311
           WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG
Sbjct: 241 WLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQG 300

Query: 312 RDEILLHIAQLRNYWLKH 330
           RDEILLHIAQLRNYWLKH
Sbjct: 301 RDEILLHIAQLRNYWLKH 318

BLAST of CmaCh01G015940 vs. NCBI nr
Match: KAG7037585.1 (GTP-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 306/312 (98.08%), Postives = 308/312 (98.72%), Query Frame = 0

Query: 18  SKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSFSAPKSTLTAVEPVSITDFSE 77
           SKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPS  APKSTLTA EPVSITDFSE
Sbjct: 33  SKLNRSLFTSPIRRSFSVFAKTNKPIIAATEASILTSPSIPAPKSTLTAAEPVSITDFSE 92

Query: 78  YPEEQQLTISPEKLFIPPETDFSGSGAAGTRILKGSNIVLGRYARDAQISEANFVKSSVR 137
           +PEE+QLTISPEKLFIPPETDFSGSG AGTRILKGSNIVLGRYARDAQISEANFVKSSVR
Sbjct: 93  HPEEKQLTISPEKLFIPPETDFSGSGVAGTRILKGSNIVLGRYARDAQISEANFVKSSVR 152

Query: 138 TEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRINDSWYLV 197
           TEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRINDSWYLV
Sbjct: 153 TEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRINDSWYLV 212

Query: 198 DLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYASWLGQNQ 257
           DLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYASWLGQNQ
Sbjct: 213 DLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYASWLGQNQ 272

Query: 258 IPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQGRDEILL 317
           IPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQGRDEILL
Sbjct: 273 IPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTSSVTHQGRDEILL 332

Query: 318 HIAQLRNYWLKH 330
           HIAQLRNYWLKH
Sbjct: 333 HIAQLRNYWLKH 344

BLAST of CmaCh01G015940 vs. TAIR 10
Match: AT5G11480.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 5.9e-122
Identity = 224/324 (69.14%), Postives = 263/324 (81.17%), Query Frame = 0

Query: 12  MVLSQLSKLNRSLFTSP-IRRSFSVFAKTN-KPIIAATEASILTSPSFSAPKSTLTAVEP 71
           M+L+ L + + S+   P    S S F+  N KP +  +     T  SF A KS L  VEP
Sbjct: 1   MILAHLPRFHLSILAKPSFSLSSSHFSFLNPKPSVTVSR----TLFSF-ASKSNLATVEP 60

Query: 72  V--SITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAG--TRILKGSNIVLGRYARDAQ 131
           +  S++D ++  ++  + I+ +KLFIPPETD SG  +A   TRILKGSNIVL +YARDAQ
Sbjct: 61  IPLSVSDSTDL-DDAPVEIALDKLFIPPETDISGEDSASLTTRILKGSNIVLSKYARDAQ 120

Query: 132 ISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCI 191
           + +A++VKSSV+TEDCP+DGLPEFALVGRSNVGKSSLLNSLVRRK+LA TSKKPGKTQCI
Sbjct: 121 VVQADYVKSSVKTEDCPADGLPEFALVGRSNVGKSSLLNSLVRRKRLALTSKKPGKTQCI 180

Query: 192 NHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQI 251
           NHFRIND WYLVDLPGYGYASAP EL+ DWNKFTKDYFLNRSTLVSVFLL+DAS+P K I
Sbjct: 181 NHFRINDKWYLVDLPGYGYASAPHELKQDWNKFTKDYFLNRSTLVSVFLLVDASIPVKPI 240

Query: 252 DLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQELIGGFFHTTPPWIMTS 311
           DL+YASWLGQNQ+PMT+IFTKCDKRKKKK  GK+ E N+ +FQ+LI GFF TTPPWIMTS
Sbjct: 241 DLEYASWLGQNQVPMTLIFTKCDKRKKKKNGGKKPEANIKEFQDLIQGFFETTPPWIMTS 300

Query: 312 SVTHQGRDEILLHIAQLRNYWLKH 330
           SVT+QGRDEILLH+AQLRNYWLKH
Sbjct: 301 SVTNQGRDEILLHMAQLRNYWLKH 318

BLAST of CmaCh01G015940 vs. TAIR 10
Match: AT2G22870.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.5e-80
Identity = 149/277 (53.79%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 50  SILTSPSFSAPKSTLTAVEPVSITDFSEYPEEQQLTISPEKLFIPPETDFSGSGAAGTRI 109
           + ++ P  SA  +  T     SI+D +++        +   LFIPP  +          +
Sbjct: 34  NFISIPKISASTNNPTTTTNRSISDATKF--------AKSVLFIPPGVEI--EELTDDMV 93

Query: 110 LKGSNIVLGRYARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRR 169
           L GSNIV+G +A  +QI E  FVKSS R  DCP D  PE A++GRSNVGKSSL+N LVR+
Sbjct: 94  LPGSNIVIGPFAGHSQIKEVEFVKSSARARDCPKDDRPEIAILGRSNVGKSSLINCLVRK 153

Query: 170 KKLAHTSKKPGKTQCINHFRINDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTL 229
           K++A TSKKPGKTQ INHF +N SWY+VDLPGYG+A      +TDW+ FTK YFLNR +L
Sbjct: 154 KEVALTSKKPGKTQLINHFLVNKSWYIVDLPGYGFAKVSDAAKTDWSAFTKGYFLNRDSL 213

Query: 230 VSVFLLIDASVPAKQIDLDYASWLGQNQIPMTIIFTKCDKRKKKKKDGKRAEENMSDFQE 289
           V V LLIDASVP ++IDLD A+WLG+N +PMT +FTKCDK K  K  GKR +EN+  FQ+
Sbjct: 214 VCVLLLIDASVPPQKIDLDCANWLGRNNVPMTFVFTKCDKMKATK--GKRPDENIKAFQQ 273

Query: 290 LIGGFFHTTPPWIMTSSVTHQGRDEILLHIAQLRNYW 327
           +I   F   PPWI+TSSV+  GRDE+LLH++QLRNYW
Sbjct: 274 IIRENFKVHPPWILTSSVSGLGRDELLLHMSQLRNYW 298

BLAST of CmaCh01G015940 vs. TAIR 10
Match: AT5G58370.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 6.2e-23
Identity = 57/138 (41.30%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 131 FVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRI 190
           F  ++V +   P D +PE A  GRSNVGKSSLLN+L R+  +  TS KPG TQ IN F +
Sbjct: 274 FAAANVSSSFPPPD-VPEIAFAGRSNVGKSSLLNALTRQWGVVRTSDKPGLTQTINFFGL 333

Query: 191 NDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYA 250
                LVDLPGYG+A A  E++  W    K+Y   R++L  V LL+D     K  D +  
Sbjct: 334 GPKVRLVDLPGYGFAFAKDEVKEAWEDLVKEYVSTRTSLKRVCLLVDTKWGMKPRDQELI 393

Query: 251 SWLGQNQIPMTIIFTKCD 269
           + + ++     I+ TK D
Sbjct: 394 NLMERSNTKYQIVLTKTD 410

BLAST of CmaCh01G015940 vs. TAIR 10
Match: AT5G58370.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 6.2e-23
Identity = 57/138 (41.30%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 131 FVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLAHTSKKPGKTQCINHFRI 190
           F  ++V +   P D +PE A  GRSNVGKSSLLN+L R+  +  TS KPG TQ IN F +
Sbjct: 274 FAAANVSSSFPPPD-VPEIAFAGRSNVGKSSLLNALTRQWGVVRTSDKPGLTQTINFFGL 333

Query: 191 NDSWYLVDLPGYGYASAPRELRTDWNKFTKDYFLNRSTLVSVFLLIDASVPAKQIDLDYA 250
                LVDLPGYG+A A  E++  W    K+Y   R++L  V LL+D     K  D +  
Sbjct: 334 GPKVRLVDLPGYGFAFAKDEVKEAWEDLVKEYVSTRTSLKRVCLLVDTKWGMKPRDQELI 393

Query: 251 SWLGQNQIPMTIIFTKCD 269
           + + ++     I+ TK D
Sbjct: 394 NLMERSNTKYQIVLTKTD 410

BLAST of CmaCh01G015940 vs. TAIR 10
Match: AT1G08410.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.7e-04
Identity = 32/96 (33.33%), Postives = 45/96 (46.88%), Query Frame = 0

Query: 111 KGSNIVLGRYARDAQISEANFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRK 170
           +   IV  R +R A +S  ++     R +            VG  NVGKSS +N+LV +K
Sbjct: 280 EAQEIVKVRNSRAASVSSQSWTGEYQRDQ-------AVVGFVGYPNVGKSSTINALVGQK 339

Query: 171 KLAHTSKKPGKTQCINHFRINDSWYLVDLPGYGYAS 207
           +   TS  PGKT+      I+D   L D PG  + S
Sbjct: 340 RTGVTS-TPGKTKHFQTLIISDELMLCDCPGLVFPS 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810042.1e-7953.79GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV... [more]
A5FIZ13.3e-4543.90Probable GTP-binding protein EngB OS=Flavobacterium johnsoniae (strain ATCC 1706... [more]
A6L4J31.3e-4445.64Probable GTP-binding protein EngB OS=Bacteroides vulgatus (strain ATCC 8482 / DS... [more]
Q8A8T42.1e-4444.95Probable GTP-binding protein EngB OS=Bacteroides thetaiotaomicron (strain ATCC 2... [more]
Q5LDL23.7e-4445.45Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / D... [more]
Match NameE-valueIdentityDescription
A0A6J1J1E81.1e-184100.00GTP-binding protein At2g22870-like OS=Cucurbita maxima OX=3661 GN=LOC111480403 P... [more]
A0A6J1FJT12.4e-17698.11GTP-binding protein At2g22870-like OS=Cucurbita moschata OX=3662 GN=LOC111445985... [more]
A0A0A0LGU25.3e-15586.48EngB-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G888580... [more]
A0A5A7T5H01.0e-15386.16GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G... [more]
A0A6J1IKL51.3e-15384.88GTP-binding protein At2g22870-like OS=Cucurbita maxima OX=3661 GN=LOC111476474 P... [more]
Match NameE-valueIdentityDescription
XP_022981183.12.3e-184100.00GTP-binding protein At2g22870-like [Cucurbita maxima][more]
XP_022940339.15.1e-17698.11GTP-binding protein At2g22870-like [Cucurbita moschata][more]
XP_023524307.11.6e-17497.80GTP-binding protein At2g22870-like [Cucurbita pepo subsp. pepo][more]
KAG6608233.11.8e-17396.86GTP-binding protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7037585.11.2e-17298.08GTP-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G11480.15.9e-12269.14P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G22870.11.5e-8053.79P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G58370.16.2e-2341.30P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G58370.26.2e-2341.30P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G08410.11.7e-0433.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 149..266
e-value: 9.4E-22
score: 77.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 120..325
e-value: 1.7E-53
score: 183.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 141..325
IPR019987GTP-binding protein, ribosome biogenesis, YsxCTIGRFAMTIGR03598TIGR03598coord: 129..319
e-value: 6.2E-59
score: 196.8
IPR019987GTP-binding protein, ribosome biogenesis, YsxCHAMAPMF_00321GTPase_EngBcoord: 134..323
score: 24.551668
NoneNo IPR availablePANTHERPTHR11649:SF75PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 64..326
NoneNo IPR availablePANTHERPTHR11649MSS1/TRME-RELATED GTP-BINDING PROTEINcoord: 64..326
IPR030393EngB-type guanine nucleotide-binding (G) domainPROSITEPS51706G_ENGBcoord: 145..325
score: 36.769508
IPR030393EngB-type guanine nucleotide-binding (G) domainCDDcd01876YihA_EngBcoord: 148..322
e-value: 1.26736E-65
score: 202.355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G015940.1CmaCh01G015940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005525 GTP binding
molecular_function GO:0046872 metal ion binding