CmaCh01G015850 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh01G015850 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 421.8 bits (1083), Expect = 1.7e-116
Identity = 280/742 (37.74%), Postives = 397/742 (53.50%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVF-LVHV-----KLRSPSNSSL---KMKCDPE 79
           V VA+     S+  + WAI+     G   F L+H+      + +P  +++   +++ D  
Sbjct: 22  VVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVV 81

Query: 80  AGVR--------ELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGAS 139
              R        E+  P+     R+ +  + +V+E  ++A A+ E     +I+ IV+G S
Sbjct: 82  TAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGS 141

Query: 140 SKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMR-SASRPAPVVNPLRSQLVQQS 199
           S+S F R    D+   +S   P+FC V+V+SKGK+  +R S S     +    S+    S
Sbjct: 142 SRSFFSR--KADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSS 201

Query: 200 STKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSFQ 259
           S  S  +       +SA DS   RP+ LP R           F             SS  
Sbjct: 202 SGSSGPTSDSSDVMSSAHDS-QSRPLSLPVRRMQH-------FPAIAGQASVPMETSSVG 261

Query: 260 ESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPR----------MSSSTNLDLTRSFES 319
             +   +S        +      S D   R TP R           SSS+N +       
Sbjct: 262 SDETRCMSLDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGTR 321

Query: 320 LPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEA--EMRRLKLELKQTMEMYSTAC 379
                + +D          S  SD LS  S +   V    E+ +L+ EL+   EMY+ A 
Sbjct: 322 FSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQ 381

Query: 380 KEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAE 439
            E   A +K  EL + +LEE  ++EE +L    A  LAE+EK   + A   AE+ R  AE
Sbjct: 382 TETFDASRKLGELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAE 441

Query: 440 LEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGY 499
            E  +R +AE K+ ++ +EK++    L    ++Y+ +  EEI  AT  F+E  KIG G Y
Sbjct: 442 REIAQRREAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAY 501

Query: 500 GPVYNCDLDHTPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYE 559
           G VY C+L HT   +KVL+    Q   QF+QE+E+LS IRHP++VLLLGACPE G LVYE
Sbjct: 502 GAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYE 561

Query: 560 FMANGSLEDRLLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLD 619
           +M NGSLEDRL +  N+PPL W  RF+IA E+  AL+FLH++KP+P++HRDLKPANILLD
Sbjct: 562 YMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLD 621

Query: 620 RNFVSKISDVGLARLVP-PSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLG 679
            NFVSK+ DVGL+ +V    ++   T Y+ TS  GT CYIDPEYQ+TG +  KSDIYS G
Sbjct: 622 HNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFG 681

Query: 680 IMFLQLITSRPPMGLTHHVERAIE-NDMFANILDPAVTDWPVEEALSFAKLSLKCAELRR 730
           ++ LQL+T++P + LTH VE A++ ND F  ILD    +WP+EE    A L+L C ELR 
Sbjct: 682 MILLQLLTAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRG 741

BLAST of CmaCh01G015850 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.2e-116
Identity = 290/781 (37.13%), Postives = 419/781 (53.65%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVF-LVHVK------------------LRSPSN 79
           VAVAI+  K S+  + WA++  +  G T F L++V+                  LR    
Sbjct: 24  VAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDVV 83

Query: 80  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 139
           S+ K + D  A   E+  P++    R+ +Q + ++L+  + A A+ E  A T +  +V+G
Sbjct: 84  SAYKQELDWSA--NEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIG 143

Query: 140 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 199
            S +  F R    D+   ++   P FC V+VISKGK+ S+R +               + 
Sbjct: 144 MSLRGFFSR--KIDMSSLIATAVPRFCTVYVISKGKLASVRPS---------------ES 203

Query: 200 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMR---------SPFTRKGYNY 259
            ++ SIR    F +++S   S D      PR   +  DF+          SPF+      
Sbjct: 204 DASGSIR----FERSSSTSGSTDS-----PRLPPEYQDFLSAVSEAQSRVSPFSPA---L 263

Query: 260 KPSYG-------DSSFQESDISYVSSGR----------------PSIDRIFP---SVYDS 319
           K S G       D+S   +D   VS+GR                 S    FP     Y+S
Sbjct: 264 KHSMGSNAVAQMDTSSSGTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNS 323

Query: 320 L-------DMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMDMN--FPSEFSSISQDSDR 379
           +       D   R      SSS N DL           ++MD     P  +S +S  +  
Sbjct: 324 MSWTSKWRDHEDRREMRSSSSSNNHDL-----------VNMDWGAVVPENYSWVSHTASH 383

Query: 380 LS---ISSQSLED----VEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEE 439
           +S   +S  S+ D    +  E+ +L+ ELK   EMY+ A  E + A +K  EL + + EE
Sbjct: 384 MSDGLLSVHSITDNQVNLNFEIEKLRAELKHVQEMYAMAQTETVGASKKLTELNQRRFEE 443

Query: 440 EQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEK 499
            +++ E +   E A   A +EK + + A++ AE  + +   EA  R +AE KA ++A EK
Sbjct: 444 SEKLVELKEKEEVAKDTASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREK 503

Query: 500 KRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHTPVAIKVLRP 559
            +   +L    ++Y+ YT EEI  AT  FAE+ KIG G YG VY C+L HT  A+KVL  
Sbjct: 504 DKLQASLVSPGVQYQHYTWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHA 563

Query: 560 DATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRRGNTPPL 619
             TQ   QF QE+E+LS IRHP++VLLLGACPE GCLVYE+M NGSL+DRL+   +TPP+
Sbjct: 564 GETQLSKQFDQELEILSKIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPI 623

Query: 620 SWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSV 679
            W  RF+IA E+ +AL+FLH++KP P++HRDLKP NILLD NFVSK+ DVGL+ +V    
Sbjct: 624 PWFERFRIALEVASALVFLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDD 683

Query: 680 ADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVER 730
             + T ++ TS  GT CYIDPEYQ+TG++  KSD+YSLG++ LQLIT++P + +TH VE 
Sbjct: 684 VSSRTIFKQTSPVGTLCYIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEE 743

BLAST of CmaCh01G015850 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 412.9 bits (1060), Expect = 7.8e-114
Identity = 281/736 (38.18%), Postives = 402/736 (54.62%), Query Frame = 0

Query: 20  VAVAI-DKDKGSQGALKWAIDHLLRRGQTVF-LVHVKLRSPSNSSLK--------MKCDP 79
           VAVAI   +  ++G ++WA+     +   VF L+HV+ R  ++ S           K D 
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTTSVYKKDV 67

Query: 80  EAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGF-L 139
           +   RE+ LP R     +++Q   +VLE  DIA A+++   +  I  +V+GASS   F  
Sbjct: 68  DRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFSW 127

Query: 140 RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRP-----APVVNPLRSQLVQQSST 199
           + K +++   ++   P FC+V VISKGK+ ++R +        A   +  R      S T
Sbjct: 128 KLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSESRFSSDSHSGT 187

Query: 200 KSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSFQES 259
            S  S +QF  T          P+ L +R Q  T   +   T  G      +     +  
Sbjct: 188 VSSTSSHQFSST----------PL-LFQRIQALTTVNQKVGTNIGKQNNEPHHHHHNRAG 247

Query: 260 DISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMDMNF 319
            +    S   +    + +    +  GG             D++ S+ S  M         
Sbjct: 248 SLDVDESKLLNQKGFYRTSSSGIGYGGS------------DIS-SWRSSQM--------- 307

Query: 320 PSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQ 379
             E SS S  SD  S SSQ  +D E E  +LK+EL+    MY+ A  E + A +K  +L 
Sbjct: 308 -EEASSSSTYSDPTSSSSQIHKDFELE--KLKIELRHIKGMYAVAQSEVIDASKKMQDLN 367

Query: 380 RWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAH 439
           + + EE  R++   +  E A  + E E+ + + A   AE  R   E E ++R++AE +A 
Sbjct: 368 QRRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAE 427

Query: 440 KEAEEKKRAIDALSQSDI---RYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 499
           +  +EK+R  DAL    +   +Y K+  EEI EAT  F++  KIG GGYG VY C+L HT
Sbjct: 428 EVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHT 487

Query: 500 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 559
            VA+KVL  D +    QF QE+E+LS IRHP+++LLLGACPE G LVYE+M NGSLE+RL
Sbjct: 488 TVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERL 547

Query: 560 LRR------GNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVS 619
           ++R         PPL W  RF+IA EI +AL FLH  +P P+VHRDLKPANILLDRN VS
Sbjct: 548 MKRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVS 607

Query: 620 KISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQL 679
           KI DVGL+++V    +   T +  T   GTF YIDPEYQ+TG++  +SDIY+ GI+ LQL
Sbjct: 608 KIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQL 667

Query: 680 ITSRPPMGLTHHVERAI--ENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPD 729
           +T+R  MGL H +E+A+  +   F  ILD    DWPV+EA     + L+CAE+R++DRPD
Sbjct: 668 VTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPD 707

BLAST of CmaCh01G015850 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 6.6e-105
Identity = 266/723 (36.79%), Postives = 381/723 (52.70%), Query Frame = 0

Query: 19  FVAVAI-----DK--DKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSNSSLKMKCDPEA 78
           FVAVA+     DK    GS+ A++WA+D+LL +     ++HV    P+ +S+     P  
Sbjct: 23  FVAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHV---IPTITSI-----PTP 82

Query: 79  GVRELFLPFRCFCTRKD------IQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKS 138
            +  L        T  D      ++   V +   D+ K   E      ++      S+K 
Sbjct: 83  NILILMFTRMWVVTAGDRLPVEEVEESVVEMYVRDVKKEY-ETVFVPFLKMCKSTRSTKR 142

Query: 139 GF--LRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVN--PLRSQLVQQS 198
            F   R K T VP  V + AP+ C V+++ K +I    +     P++N  P  S     +
Sbjct: 143 YFRSRRTKGTGVPLTVLRYAPETCEVYIVCKDRI----TTKSMDPLINREPCTSPHAAAT 202

Query: 199 STKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSFQ 258
           +   +R         ++  +L    +  PR+S           T  G     S  +  F+
Sbjct: 203 AHDFLR------DWAASFHTLRSPTLPDPRQS-----------TEAGTRRSASARELRFE 262

Query: 259 ESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMDM 318
              ++      P   +   +    +    R +     + ++ D T    + P   +    
Sbjct: 263 ALSLTCNKPKTPQSSKASSATTPEIFRRRRGSDIPQLNYSDFDKTC---TKPQSNVE--- 322

Query: 319 NFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVE 378
           N  SE     +     S  S+ +E +E E+ RLK EL+ T+  Y  AC+E  + + K   
Sbjct: 323 NIVSEHRDSDRSPPETSRKSKKVE-IEEEVERLKNELQSTVFKYKQACEELFSTQNKVKM 382

Query: 379 LQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMK 438
           L    L E +R+  A    E     A  EK +   A++  E A+ +   E  +R  AE+ 
Sbjct: 383 LSTEYLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVN 442

Query: 439 AHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHTP 498
           A +   EKK+ ID L  +D RYRKYTIEEI  ATE F+  + IGEGGYG VY C LD TP
Sbjct: 443 ALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTP 502

Query: 499 VAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLL 558
            A+KV+R D  + + +F +EVEVLS +RHP++VLLLGACPE GCLVYE++ NGSLE+ + 
Sbjct: 503 AAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIF 562

Query: 559 RRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGL 618
            R N PPL W +RF++  E+   L FLH +KPEP+VHRDLKP NILL+RN+VSKI+DVGL
Sbjct: 563 HRKNKPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGL 622

Query: 619 ARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPM 678
           A+LV     DNVT YR +  AGT  YIDPEY +TG +  KSD+Y+ GI+ LQL+T+R P 
Sbjct: 623 AKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTARNPS 682

Query: 679 GLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPEL 725
           G+   VE A++      +LD +VTDWP+ E    A++ LKCAE R +DRPDL  EV+P L
Sbjct: 683 GIVPAVENAVKKGTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVIPVL 708

BLAST of CmaCh01G015850 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.3e-97
Identity = 263/751 (35.02%), Postives = 391/751 (52.06%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHL-LRRGQTVFLVHV-----KLRSPSNSSLKMKCDPE--- 79
           VA+AI     S+  +KWA++     +  T  L+H+      L + S + + +  + E   
Sbjct: 34  VALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSISEELEEVA 93

Query: 80  AGVRE---------LFLPFRCFCTRKDIQCKDV--------------VLEDTDIAKALTE 139
           A  R+         L  PF+  C RK ++  +               VLE   +A A+T+
Sbjct: 94  AAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNSVAVAITK 153

Query: 140 YAANTAIENIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSM-RSASRP 199
                 I N+++G SS++   R    D+  ++S    + C V+V+S G +  + +  S  
Sbjct: 154 EVNQHLISNLIIGRSSQAASSR--NYDITASISASVSNLCTVYVVSNGGVHILAKDTSDT 213

Query: 200 APVVNPLRSQLVQQSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTR 259
                 + S   + SS+ S  S       ++A   L   P  L  +          P   
Sbjct: 214 ERNDTSIESGFERTSSSCSSGSGANSDVMSNA---LKSNPHTLSNKRMQNL-----PTIV 273

Query: 260 KGYNYKPSYGDSSFQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLD-L 319
           +G +      ++S  ESD +   S             D+ +   + + P  S S + +  
Sbjct: 274 RGVSVPM---ETSSTESDETKKRSS------------DAAEEASKRSSPETSRSVSWNPQ 333

Query: 320 TRSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMY 379
            R F+       SM  NF  E+ ++          +Q   D   E+ +L+ EL+   EMY
Sbjct: 334 FRDFDERKDAMSSMSSNF--EYGNVVTPLGHYFTDNQ---DTLNEISKLRAELRHAHEMY 393

Query: 380 STACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQE-KAKSKAAIEAAEAA 439
           + A  E L A +K  EL   K EE              L L E E K  +K   E  E  
Sbjct: 394 AVAQVETLDASRKLNEL---KFEE--------------LTLLEHETKGIAKKETEKFEQK 453

Query: 440 RRIAELEAQKRMKAEMKAHKEAEEKKRAID-ALSQSDIRYRKYTIEEIEEATEFFAESRK 499
           RR  E EA +R +AEMKA  EA+EK++  + +L    ++Y+++T EEI  AT  F+E  K
Sbjct: 454 RR-EEREAAQRREAEMKATHEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLK 513

Query: 500 IGEGGYGPVYNCDLDHTPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEY 559
           IG G YG VY C+L HT  A+KVL    +    QF QE+E+LS IRHP++VLLLGACP++
Sbjct: 514 IGMGAYGDVYKCNLHHTIAAVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDH 573

Query: 560 GCLVYEFMANGSLEDRLLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKP 619
           G LVYE+M NGSLEDRL +  ++ P+ W +R +IA E+ +AL+FLH++KP P++HRDLKP
Sbjct: 574 GALVYEYMENGSLEDRLFQVNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKP 633

Query: 620 ANILLDRNFVSKISDVGLARLVPPS--VADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVK 679
           ANILL+ NFVSK+ DVGL+ ++  +  ++   T Y+ TS  GT CYIDPEYQ+TG +  K
Sbjct: 634 ANILLNHNFVSKVGDVGLSTMIQAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPK 693

Query: 680 SDIYSLGIMFLQLITSRPPMGLTHHVERAIEN---DMFANILDPAVTDWPVEEALSFAKL 730
           SD+Y+ G++ LQL+T +  M LT+ VE A+EN   D    ILD    +WP+EE    A L
Sbjct: 694 SDVYAFGMIILQLLTGQQAMALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAAL 736

BLAST of CmaCh01G015850 vs. ExPASy TrEMBL
Match: A0A6J1IYC9 (U-box domain-containing protein 35-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481038 PE=4 SV=1)

HSP 1 Score: 1489.9 bits (3856), Expect = 0.0e+00
Identity = 767/767 (100.00%), Postives = 767/767 (100.00%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240
           SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF
Sbjct: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300
           QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480
           KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480

Query: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720

Query: 721 LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR
Sbjct: 721 LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 767

BLAST of CmaCh01G015850 vs. ExPASy TrEMBL
Match: A0A6J1J1M6 (U-box domain-containing protein 52-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481038 PE=4 SV=1)

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 767/768 (99.87%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS 240
           QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS
Sbjct: 181 QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM 300
           FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH 480
           MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH 480

Query: 481 TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP 720

Query: 721 ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR
Sbjct: 721 ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768

BLAST of CmaCh01G015850 vs. ExPASy TrEMBL
Match: A0A6J1FML4 (U-box domain-containing protein 52-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446783 PE=4 SV=1)

HSP 1 Score: 1457.6 bits (3772), Expect = 0.0e+00
Identity = 750/767 (97.78%), Postives = 759/767 (98.96%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240
           SSTKSI SDYQFPQTTSAR SLDKRP  LPRRSQDE DFMRSPFTRKGYNYKPSYGDSSF
Sbjct: 181 SSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300
           QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480
           KAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480

Query: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           L+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK+SLKCAELRRKDRPDLGKE+LPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           LNRLRTIAEDY+HPTLMGDISPTRSSSQVS+PLGMMSSSPQSY+SSR
Sbjct: 721 LNRLRTIAEDYIHPTLMGDISPTRSSSQVSLPLGMMSSSPQSYNSSR 767

BLAST of CmaCh01G015850 vs. ExPASy TrEMBL
Match: A0A6J1FLA1 (U-box domain-containing protein 52-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446783 PE=4 SV=1)

HSP 1 Score: 1453.0 bits (3760), Expect = 0.0e+00
Identity = 750/768 (97.66%), Postives = 759/768 (98.83%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS 240
           QSSTKSI SDYQFPQTTSAR SLDKRP  LPRRSQDE DFMRSPFTRKGYNYKPSYGDSS
Sbjct: 181 QSSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM 300
           FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH 480
           MKAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480

Query: 481 TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LL+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK+SLKCAELRRKDRPDLGKE+LP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720

Query: 721 ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           ELNRLRTIAEDY+HPTLMGDISPTRSSSQVS+PLGMMSSSPQSY+SSR
Sbjct: 721 ELNRLRTIAEDYIHPTLMGDISPTRSSSQVSLPLGMMSSSPQSYNSSR 768

BLAST of CmaCh01G015850 vs. ExPASy TrEMBL
Match: A0A6J1IVP7 (U-box domain-containing protein 35-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481038 PE=4 SV=1)

HSP 1 Score: 1354.7 bits (3505), Expect = 0.0e+00
Identity = 703/704 (99.86%), Postives = 703/704 (99.86%), Query Frame = 0

Query: 65  MKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSK 124
           MKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSK
Sbjct: 1   MKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSK 60

Query: 125 SGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQSST 184
           SGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQSST
Sbjct: 61  SGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQSST 120

Query: 185 KSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSFQES 244
           KSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSFQES
Sbjct: 121 KSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSFQES 180

Query: 245 DISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMDMNF 304
           DISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMDMNF
Sbjct: 181 DISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMDMNF 240

Query: 305 PSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQ 364
           PSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQ
Sbjct: 241 PSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQ 300

Query: 365 RWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAH 424
           RWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAH
Sbjct: 301 RWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAH 360

Query: 425 KEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHTPVA 484
           KEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHTPVA
Sbjct: 361 KEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHTPVA 420

Query: 485 IKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRR 544
           IKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRR
Sbjct: 421 IKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRR 480

Query: 545 GNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLAR 604
           GNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLAR
Sbjct: 481 GNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLAR 540

Query: 605 LVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGL 664
           LVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGL
Sbjct: 541 LVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGL 600

Query: 665 THHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPELNR 724
           THHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPELNR
Sbjct: 601 THHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPELNR 660

Query: 725 LRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           LRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR
Sbjct: 661 LRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 704

BLAST of CmaCh01G015850 vs. NCBI nr
Match: XP_022982091.1 (U-box domain-containing protein 35-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1489.9 bits (3856), Expect = 0.0e+00
Identity = 767/767 (100.00%), Postives = 767/767 (100.00%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240
           SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF
Sbjct: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300
           QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480
           KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480

Query: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720

Query: 721 LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR
Sbjct: 721 LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 767

BLAST of CmaCh01G015850 vs. NCBI nr
Match: XP_022982090.1 (U-box domain-containing protein 52-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 767/768 (99.87%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS 240
           QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS
Sbjct: 181 QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM 300
           FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH 480
           MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH 480

Query: 481 TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP 720

Query: 721 ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR
Sbjct: 721 ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768

BLAST of CmaCh01G015850 vs. NCBI nr
Match: XP_022941502.1 (U-box domain-containing protein 52-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1457.6 bits (3772), Expect = 0.0e+00
Identity = 750/767 (97.78%), Postives = 759/767 (98.96%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240
           SSTKSI SDYQFPQTTSAR SLDKRP  LPRRSQDE DFMRSPFTRKGYNYKPSYGDSSF
Sbjct: 181 SSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300
           QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480
           KAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480

Query: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           L+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK+SLKCAELRRKDRPDLGKE+LPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           LNRLRTIAEDY+HPTLMGDISPTRSSSQVS+PLGMMSSSPQSY+SSR
Sbjct: 721 LNRLRTIAEDYIHPTLMGDISPTRSSSQVSLPLGMMSSSPQSYNSSR 767

BLAST of CmaCh01G015850 vs. NCBI nr
Match: XP_022941501.1 (U-box domain-containing protein 52-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1453.0 bits (3760), Expect = 0.0e+00
Identity = 750/768 (97.66%), Postives = 759/768 (98.83%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS 240
           QSSTKSI SDYQFPQTTSAR SLDKRP  LPRRSQDE DFMRSPFTRKGYNYKPSYGDSS
Sbjct: 181 QSSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM 300
           FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH 480
           MKAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480

Query: 481 TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LL+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK+SLKCAELRRKDRPDLGKE+LP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720

Query: 721 ELNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDSSR 768
           ELNRLRTIAEDY+HPTLMGDISPTRSSSQVS+PLGMMSSSPQSY+SSR
Sbjct: 721 ELNRLRTIAEDYIHPTLMGDISPTRSSSQVSLPLGMMSSSPQSYNSSR 768

BLAST of CmaCh01G015850 vs. NCBI nr
Match: XP_023524077.1 (U-box domain-containing protein 52-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1426.4 bits (3691), Expect = 0.0e+00
Identity = 736/765 (96.21%), Postives = 749/765 (97.91%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240
           SSTKSIRSDYQFPQTTSAR SLDKRP  LPR SQDE DFMRSPFTRKGYNYKPSYGDSSF
Sbjct: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRGSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300
           QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALA+QEK KSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAKQEKTKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480
           KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYRCDLDHT 480

Query: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           L+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720
           MGLTHHVE+AIENDMFANILDPA+TDWP+EEALSFAK+SLKCAELRRKDRPDLGKE+LPE
Sbjct: 661 MGLTHHVEQAIENDMFANILDPAITDWPIEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYMHPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDS 766
           LNRLRTIAEDY+HPTLMGDISPTRSSSQVS+ L +  S P S+ S
Sbjct: 721 LNRLRTIAEDYIHPTLMGDISPTRSSSQVSLQL-VSLSLPLSFTS 764

BLAST of CmaCh01G015850 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 973.8 bits (2516), Expect = 8.2e-284
Identity = 524/791 (66.25%), Postives = 626/791 (79.14%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLR-SPS 60
           MW+P+ +    +    +G VAVAIDKDK SQ ALKWA+D+LL+RGQ+V LVHVKLR SP 
Sbjct: 1   MWMPKNNNVGVKEGGSSGVVAVAIDKDKSSQHALKWAVDNLLQRGQSVILVHVKLRPSPL 60

Query: 61  NSSLKMKC--------------DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKAL 120
           N+S  +                DPE   +E+FLPFRCFCTRKDIQC+DV+LE++D+AKAL
Sbjct: 61  NNSASLHASSAKLSQDSSLVCRDPEGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKAL 120

Query: 121 TEYAANTAIENIVVGASSKSGFLRF-KATDVPGNVSKGAPDFCNVFVISKGKIQSMRSAS 180
            EY    AIE +VVG+SSK GFLRF K TD+PG+++K APDFC V++ISKGKIQ+MRSAS
Sbjct: 121 VEYVNQAAIEVLVVGSSSKGGFLRFNKPTDIPGSITKNAPDFCTVYIISKGKIQTMRSAS 180

Query: 181 RPAPVVNPLRS----------QLVQQSSTKSIRSD---YQFPQTTSARDSLDKRPIDLPR 240
           R AP+  PLRS          Q +  +S  S+R+D   ++  Q  S  D   +   D  R
Sbjct: 181 RSAPMTAPLRSSVQPPSLKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQR 240

Query: 241 RS-QDETDFMRSPFTRKGYNYKPSYGDSSFQESDISYVSSGRPSIDRIFPSVYDSLDMGG 300
           RS  D+++  RSPFTR+G     SYGD +  ESDIS++SSGRPSIDRIFPS+YD+ D   
Sbjct: 241 RSIDDQSESFRSPFTRRGNG--RSYGDLTVPESDISFISSGRPSIDRIFPSLYDNNDPS- 300

Query: 301 RATPPRMSSSTNLDLTRSF-ESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEA 360
             TPPR+S+ +++D + S  +S   GR S+DMN P++F     +S+R S +SQS++DVEA
Sbjct: 301 -RTPPRLSNFSDMDYSSSLDQSSNYGRRSVDMNSPTDF-----ESERFSSASQSIDDVEA 360

Query: 361 EMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQ 420
           EMRRLKLELKQTMEMYSTACKEALTAKQKA ELQRWKLEEE+++EEAR A EAALA+AE+
Sbjct: 361 EMRRLKLELKQTMEMYSTACKEALTAKQKATELQRWKLEEERKLEEARNAEEAALAIAEK 420

Query: 421 EKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIE 480
           EKAKSKAA+EAAEAA+RIAELEA+KR+ AEMKA KE+EEK +A+ AL+ SD+RYRKY+IE
Sbjct: 421 EKAKSKAAMEAAEAAQRIAELEAKKRVNAEMKALKESEEKTKALTALANSDVRYRKYSIE 480

Query: 481 EIEEATEFFAESRKIGEGGYGPVYNCDLDHTPVAIKVLRPDATQGRLQFKQEVEVLSCIR 540
           +IE ATEFFAE  KIGEGGYGPVY C LDHTPVA+KVLRPDA QGR QF+QEVEVLSCIR
Sbjct: 481 DIELATEFFAEKYKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSCIR 540

Query: 541 HPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRRGNTPPLSWQLRFQIAGEIGTALLFLH 600
           HPNMVLLLGACPE GCLVYEFMANGSLEDRL R GN+PPLSWQ+RF+IA EIGT LLFLH
Sbjct: 541 HPNMVLLLGACPECGCLVYEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAEIGTGLLFLH 600

Query: 601 QTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYID 660
           Q KPEPLVHRDLKP NILLDRNFVSKISDVGLARLVPP+VAD VTQYRMTSTAGTFCYID
Sbjct: 601 QAKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCYID 660

Query: 661 PEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPV 720
           PEYQQTGMLGVKSDIYSLGIMFLQLIT++PPMGLTH+VERA+E     ++LDP V+DWP+
Sbjct: 661 PEYQQTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHYVERALEKGTLVDLLDPVVSDWPM 720

Query: 721 EEALSFAKLSLKCAELRRKDRPDLGKEVLPELNRLRTIAEDYMHPTLMGDIS-PTRSSSQ 760
           E+   FAKL+LKCAELRRKDRPDL K +LPELNRLRT+A++  H  ++G+   P+ + SQ
Sbjct: 721 EDTEEFAKLALKCAELRRKDRPDLAKVILPELNRLRTLADESSHSVVVGNSPLPSPTGSQ 780

BLAST of CmaCh01G015850 vs. TAIR 10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 898.7 bits (2321), Expect = 3.3e-261
Identity = 483/765 (63.14%), Postives = 592/765 (77.39%), Query Frame = 0

Query: 9   DRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPS--------- 68
           DR     V+G VAVAID+DK SQGALKWA+D+LL++GQTV LVHVK R+ S         
Sbjct: 7   DRDGGFGVDGLVAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLSTNPSINS 66

Query: 69  --------NSSLKMKC-DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAA 128
                   N    + C +PE   ++LFLPFRC C+RKDIQCKDV+LE++D+A+AL EYA 
Sbjct: 67  NSSKTSQINGDSSLVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYAN 126

Query: 129 NTAIENIVVGASSKSGFLRF-KATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPV 188
              IE +VVG+SSK GFLRF K  D+PG ++K APDFC V+ I+KGK+ + ++ASR AP 
Sbjct: 127 QVMIEVLVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAITKGKLSTKKTASRAAPS 186

Query: 189 VNPLRSQLVQQSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRS-QDETDFMRSPFTRKG 248
           V+PLR QL Q               T + R S + +     RRS  D++D  R P+ ++G
Sbjct: 187 VSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFESQ----HRRSLDDQSDSFRPPYNKRG 246

Query: 249 YNYKPSYGDSSFQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRS 308
              + SYG+ S  +S+IS+ SSGRPSI+R  PS+YD+ D     TPPR+S+ +++D   S
Sbjct: 247 LTGR-SYGELSIPDSEISFNSSGRPSIERNSPSLYDNSD--PNRTPPRLSNFSDVDYC-S 306

Query: 309 FESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTA 368
           FES+  GR SMD++ P+ FS+ S +++R S +SQ  +DVEAEMRRLKLELKQTMEMYSTA
Sbjct: 307 FESMTFGRRSMDLSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELKQTMEMYSTA 366

Query: 369 CKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIA 428
           CKEALTAK KA ELQRWKL EE++ EEA+LA EAALA+AE+EKAKSKAA+EAAEAA+RIA
Sbjct: 367 CKEALTAKHKATELQRWKLAEERKFEEAKLAEEAALAIAEKEKAKSKAAMEAAEAAQRIA 426

Query: 429 ELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGG 488
           ++E++KR+ AE KA KE+E + +A++AL++ D+RYRKY+IEEIE+ATEFF +  KIGEG 
Sbjct: 427 DIESRKRVDAETKALKESEARTKAVNALAK-DVRYRKYSIEEIEDATEFFDDKYKIGEGS 486

Query: 489 YGPVYNCDLDHTPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVY 548
           YGPVY C LDHTPVA+K LRPDA QGR QF++EVEVL  IRHPNMVLLLGACPE GCLVY
Sbjct: 487 YGPVYKCYLDHTPVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGACPECGCLVY 546

Query: 549 EFMANGSLEDRLLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILL 608
           EFMANGSLEDRL R+G++P LSWQ RF+IA EIGT LLFLHQTKPEPLVHRDLKPANILL
Sbjct: 547 EFMANGSLEDRLFRQGDSPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHRDLKPANILL 606

Query: 609 DRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLG 668
           DRNFVSK++DVGLARLVPPSVA+ VTQY MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLG
Sbjct: 607 DRNFVSKLADVGLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLG 666

Query: 669 IMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRK 728
           IMFLQLIT +PPMGLTH+VERA+E     ++LDPAV+DWPVE+   FAKL+LKCAE+RRK
Sbjct: 667 IMFLQLITGKPPMGLTHYVERALEKGNLKDLLDPAVSDWPVEDTTEFAKLALKCAEIRRK 726

Query: 729 DRPDLGKEVLPELNRLRTIAEDYMHPTLMGDIS-PTRSSSQVSIP 753
           DRPDL K +LPELNRLR +AE+     ++ + S PT + SQ S P
Sbjct: 727 DRPDLSKVILPELNRLRVLAEESTQAAVVINSSGPTPTESQTSSP 762

BLAST of CmaCh01G015850 vs. TAIR 10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 808.5 bits (2087), Expect = 4.5e-234
Identity = 443/752 (58.91%), Postives = 562/752 (74.73%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLP+ +  +KE    +G VAVAIDKDKGSQ ALKW ID+L  RGQT+ L+HV  RS S+
Sbjct: 1   MWLPKANGAKKE--TGSGSVAVAIDKDKGSQHALKWTIDNLASRGQTISLIHVLCRSHSS 60

Query: 61  SSL-----KMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIE 120
           S L     + K   E   ++LF+ F C+C+RK+I C+D++LED D  +A+TEY +++AIE
Sbjct: 61  SDLEEGTPQQKQQMEKIAKDLFVSFHCYCSRKEINCRDILLEDADKVRAITEYVSSSAIE 120

Query: 121 NIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRS 180
           N+VVG++S++GF+R   TD+P  VSK APDFCNV+VISKGKI S+R+ASRPAP       
Sbjct: 121 NLVVGSASRNGFMRRFKTDLPTTVSKSAPDFCNVYVISKGKIASVRNASRPAP------- 180

Query: 181 QLVQQSSTKSIRSDYQFPQTTSARDSLDKRPI---DLPRRSQDETDFMRSPFTRKGYNYK 240
               Q+S +    D   P T          P      P R +   +  RSP  ++    K
Sbjct: 181 ---YQNSMQQCEIDNHHPHTPDKAPKYHDHPNSAGSTPSRPRKSVEADRSPLVKR----K 240

Query: 241 PSYGD-------------SSFQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSS 300
           P YGD             SS ++SDIS++SSGRPS++R   S + SLD    A   RMS+
Sbjct: 241 P-YGDFYDSDSDLSFISPSSHRDSDISFISSGRPSVER---SSF-SLDFPESARTSRMST 300

Query: 301 STNLDLTRSFESLPMGRLSMDMNFPSEFSSISQDSDRL-SISSQSLEDVEAEMRRLKLEL 360
           S+     +S  S  +G    D +F +E S+ S++S R  S SSQSL+DVEAEM+RL+LEL
Sbjct: 301 SSE----QSIGSNRLGIKFSDPDFLNESSTFSEESGRTSSYSSQSLDDVEAEMKRLRLEL 360

Query: 361 KQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAI 420
           KQTM+MYSTACKEAL+A+Q+A ELQ+ + EEE+R+EEA+ + EAA+++ E+E+AK+KAA+
Sbjct: 361 KQTMDMYSTACKEALSARQQATELQKLRTEEERRLEEAKSSEEAAMSIVEKERAKAKAAL 420

Query: 421 EAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFF 480
           EAAEAA+R+AE+E+++R+ AEMK  KE+       D+ S+  +RYRKYT++EIEEAT  F
Sbjct: 421 EAAEAAKRLAEVESKRRLTAEMKTMKES-------DSFSRGFVRYRKYTVDEIEEATSNF 480

Query: 481 AESRKIGEGGYGPVYNCDLDHTPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLG 540
           AES+K+GEGGYGPV+   LDHT VA+KVLRPDA QGR QF++EVEVLSCIRHPNMVLLLG
Sbjct: 481 AESQKVGEGGYGPVFRGFLDHTSVAVKVLRPDAAQGRSQFQKEVEVLSCIRHPNMVLLLG 540

Query: 541 ACPEYGCLVYEFMANGSLEDRLLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVH 600
           ACPE+G LVYE+MA GSLEDRL  RGNTPP++WQLRF+IA EI T LLFLHQTKPEP+VH
Sbjct: 541 ACPEFGILVYEYMAKGSLEDRLFMRGNTPPITWQLRFRIAAEIATGLLFLHQTKPEPIVH 600

Query: 601 RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML 660
           RDLKP N+LLD N+VSKISDVGLARLV P+VA+NVTQYR+TS AGTFCYIDPEYQQTGML
Sbjct: 601 RDLKPGNVLLDYNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGML 660

Query: 661 GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKL 720
           GVKSD+YSLGIM LQ++T++ PMGL ++VE+AIE     ++LDPAV DWP+EEALS AKL
Sbjct: 661 GVKSDVYSLGIMLLQILTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPIEEALSLAKL 719

Query: 721 SLKCAELRRKDRPDLGKEVLPELNRLRTIAED 731
           SL+CAELRRKDRPDLGKE+LPELNRLR I E+
Sbjct: 721 SLQCAELRRKDRPDLGKEILPELNRLREIGEE 719

BLAST of CmaCh01G015850 vs. TAIR 10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 768.1 bits (1982), Expect = 6.8e-222
Identity = 430/762 (56.43%), Postives = 551/762 (72.31%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60
           MWLP+   D  ++   NG VA+AIDKDK SQ A+KW +++L  RGQT+ L+HV  +S S+
Sbjct: 1   MWLPK--ADATKKGTRNGSVAIAIDKDKSSQNAIKWTLENLATRGQTLALIHVLPKSQSS 60

Query: 61  ----SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIEN 120
                 +  K   E   ++LF+ F C+C+RK+I C DVVLED D  KA+ EY   +AIEN
Sbjct: 61  LDIEEGITHKQQMEKQTKDLFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIEN 120

Query: 121 IVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQ 180
           +V+GA S++ F+R   TD+P +VSK APDFCNV+VISKGKI S+R+ASR AP    + S+
Sbjct: 121 LVLGAPSRNSFMRRFKTDLPTSVSKAAPDFCNVYVISKGKISSLRNASRLAPYHPSVLSE 180

Query: 181 LVQQSSTKSIRSDYQ---FPQTTSARDSLDK--RPIDLPRRSQDETDFMRSPFTRKGYNY 240
            V    T +I   ++    P     R S+D     + LP+        M   F+     +
Sbjct: 181 -VDDHETIAIERKHKTANTPALPKGRRSIDSDVTRLGLPKPPHGHMKLM-GDFSDSESEF 240

Query: 241 KPSYGDSSFQE-SDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFE 300
             S+  +S QE SD+S++SSGRPS+DR   S + + D+   A   RMS+S+     +S  
Sbjct: 241 --SFISASQQESSDLSFISSGRPSVDR---SSF-TYDLPESARTSRMSTSSE----QSIG 300

Query: 301 SLPMGRLSMDMNFPSEFSSISQDSDR--LSISSQSLEDVEAEMRRLKLELKQTMEMYSTA 360
           S  +G    D+++ +  SS+S +S R   S SSQSL DVEA+M+RL+LELKQTM+MYS+A
Sbjct: 301 SHRLGIKFTDLSYLNGSSSVSDESGRTSCSFSSQSLNDVEAQMKRLRLELKQTMDMYSSA 360

Query: 361 CKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIA 420
           C+EALTA+ +A ELQ+ + EEE+R+EE ++  E A+++ E E+AK+K A+EAAEAA R+A
Sbjct: 361 CREALTARNEATELQKLRTEEERRLEELKMTEETAMSIVENERAKAKTALEAAEAANRLA 420

Query: 421 ELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGG 480
           E+EA++R+ AEMK  KE++   R       S +RYRKY+++EIEE T  FAESRK+GEGG
Sbjct: 421 EVEAKRRVHAEMKVLKESDSFSR------HSIVRYRKYSVQEIEEGTANFAESRKVGEGG 480

Query: 481 YGPVYNCDLDHTPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVY 540
           YGPV+   LDHT VA+KVLRPDA QGR QF +EVEVLSCIRHPNMVLLLGACPEYG LVY
Sbjct: 481 YGPVFRGHLDHTSVAVKVLRPDAAQGRSQFHKEVEVLSCIRHPNMVLLLGACPEYGILVY 540

Query: 541 EFMANGSLEDRLLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILL 600
           E+MA GSL+DRL RRGNTPP+SWQLRF+IA EI T LLFLHQTKPEP+VHRDLKP N+LL
Sbjct: 541 EYMARGSLDDRLFRRGNTPPISWQLRFRIAAEIATGLLFLHQTKPEPIVHRDLKPGNVLL 600

Query: 601 DRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLG 660
           D N+VSKISDVGLARLV P+VA+NVTQYR+TS AGTFCYIDPEYQQTGMLGVKSD+YSLG
Sbjct: 601 DHNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLGVKSDVYSLG 660

Query: 661 IMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRK 720
           IM LQL+T++ PMGL ++VE+AIE     ++LDPAV DWP+EEALS AKLSL+CAELRRK
Sbjct: 661 IMLLQLLTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPLEEALSLAKLSLQCAELRRK 720

Query: 721 DRPDLGKEVLPELNRLRTIAEDYMHPTLMGDISPTRSSSQVS 751
           DRPDLGKEV+PEL+RLR I E+ +          +   SQVS
Sbjct: 721 DRPDLGKEVMPELSRLREIGEESLDSVYYAGQGRSSHPSQVS 741

BLAST of CmaCh01G015850 vs. TAIR 10
Match: AT5G35380.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 756.9 bits (1953), Expect = 1.6e-218
Identity = 428/753 (56.84%), Postives = 533/753 (70.78%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN--SSLKMKCDPEAGVRELF 79
           + +AID+DK SQ ALKWA+ +LL RGQT+ L+HVKL+ PS+   S      P     ELF
Sbjct: 12  ITLAIDRDKESQNALKWAVSNLLSRGQTLTLLHVKLKQPSSLPYSGSNFSKPGDDPSELF 71

Query: 80  LPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGFLRFKATDVPG 139
           LPFRC+C RKDI C+DVV+ED   AK + +Y    AIE +++G SSK   LRFKA DV  
Sbjct: 72  LPFRCYCARKDINCQDVVVEDVSAAKGIVDYVQQNAIETLILG-SSKMTLLRFKAADVSS 131

Query: 140 NVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVN--PLRSQLVQQSSTKSIRSDYQFPQT 199
            V K AP FC V+VISKGKI  +RSA+   P  N   +R     Q+S  ++    Q  Q 
Sbjct: 132 TVMKKAPSFCTVYVISKGKISFLRSATSSPPHSNMPSMRHHSHAQTSNMNVERRQQTMQR 191

Query: 200 TSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYN--YKPSYGDSSFQESDISYVSSGRP 259
           T      D+  I++                ++GY   Y+PS  D     SDIS+VSSGRP
Sbjct: 192 TH-----DEIKIEI----------------KRGYEGVYQPSITD-----SDISFVSSGRP 251

Query: 260 SIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMDMNFP-SEFSSISQ 319
           S+D++FPS+YD +D+      PR+S ++     R   +    + S+D+  P +  SS S 
Sbjct: 252 SVDQMFPSLYDDVDV------PRLSVTSEYGENRLSFATTYSKQSIDLGSPYAPNSSTSF 311

Query: 320 DSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQR 379
           +S R S S Q  +++E EMRRLK+ELK TMEMY++ACKEA++AK+ A EL +WK E+E +
Sbjct: 312 ESGRQSFSLQGQDELETEMRRLKMELKHTMEMYNSACKEAISAKKAANELLKWKAEKEHK 371

Query: 380 IEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRA 439
           +EE RL+ EAA+A+AE+EK KS+AA+EAA AA+++++LEA+KR   E       +EKKRA
Sbjct: 372 LEEVRLSKEAAMAMAEREKEKSRAAMEAAVAAQKLSDLEAEKRKHIE-----TVDEKKRA 431

Query: 440 IDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHTPVAIKVLRPDAT 499
           +     S +RYRKYTIEEIEEATE F+ SRK+GEGGYGPVY   LD+T VAIKVLRPDA 
Sbjct: 432 V-----SSLRYRKYTIEEIEEATEDFSPSRKVGEGGYGPVYKGTLDYTKVAIKVLRPDAA 491

Query: 500 QGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRRGNTPPLSWQ 559
           QGR QF++EVEVL+C+RHPNMVLLLGACPEYGCLVYE+MANGSL+D L RRGN+P LSWQ
Sbjct: 492 QGRSQFQREVEVLTCMRHPNMVLLLGACPEYGCLVYEYMANGSLDDCLFRRGNSPILSWQ 551

Query: 560 LRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADN 619
           LRF+IA EI T L FLHQ KPEPLVHRDLKP NILLD++FVSKISDVGLARLVPPSVAD 
Sbjct: 552 LRFRIASEIATGLHFLHQMKPEPLVHRDLKPGNILLDQHFVSKISDVGLARLVPPSVADT 611

Query: 620 VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIE 679
            TQYRMTSTAGTF YIDPEYQQTGMLG KSDIYS GIM LQ++T++PPMGLTHHVE+AIE
Sbjct: 612 ATQYRMTSTAGTFFYIDPEYQQTGMLGTKSDIYSFGIMLLQILTAKPPMGLTHHVEKAIE 671

Query: 680 NDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPELNRLRTIAEDYM 739
              FA +LDPAV DWP EEAL+ AKL+L+CA+LRRKDRPDLG  VLPEL +LR +AE+ +
Sbjct: 672 KGTFAEMLDPAVPDWPFEEALAAAKLALQCAKLRRKDRPDLGNIVLPELKKLRDLAEESI 720

Query: 740 HPTLMGDISPTRSSSQVSIPLGMMSSSPQSYDS 766
              +    SP RSS   +    ++S     Y S
Sbjct: 732 KFGVR-QPSPIRSSGSATSIQEIISDPQLQYGS 720