CmaCh01G014470 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G014470
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAlpha/beta-Hydrolases superfamily protein, putative
LocationCma_Chr01: 10191260 .. 10195732 (+)
RNA-Seq ExpressionCmaCh01G014470
SyntenyCmaCh01G014470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGGCAAGTTATTGGGCGAAGTATAATTTGCCGAATGTTTGAGTCACCCACCAAGAGGGTGGGTGAGGTCGAAATTCCAAACAGTTGAAAATGGAGATGGGCGGTGCTCTGTTTCTTCTACTTTTCTGCGGCCCCATTTGATTTTAGTCTTGATCAACGAGGAGCCGACAGATCCACCACTTCCAAAAGCTTCCCAAATTTACTCTCAACTTCGAAATCGCCACTCCATTTCTTCAAATCGAAGTTGCTGTATGGAAATGTGTGGAAATGAATTGTCAGAGTGGTTGCGGCAGTGAAGTTCGGGAAAATTCTGAATCGGCGGCAGAAGAGAAAATGGTGGTCTCTGCATCTTCGTCTTCCTGCAGATTCAATAGACTATGGCAATGGGTCTTCACTGTAAGCCGGTGGGGTTGCGAGTAGACGGTGTGGCCCTAGTCGTACCATTTTCCCATATAACTACTTCAACCGCTTTCAACGAAAACCTTGTTCGCACGGAAGAATTCAAGGCCTTCATTCTGCTCTGTTTTCAAACACCGACGACGCCACTTTAGCCGACTTTAGCCGACTTCTCTTCCCCTTCTTTCGTTGATTATTCTTGTTTTCTTTGGACTCTGACGTTTTCTTATGGCTTCTGTGGAATTGGGGAAGCCGAGTTATGAGGCTATGAGCCAAGGGGAAACCAGAGGACGATTGGAGAATGAGAAGAGAGAAGAAGCGAGAAATGTGATGAAGAAGAATGGAAATCGTACAGTATCGTTTTCCTTACCTATGCTCGGATGCGGATGTTTCAGAGTACAACGCGATGAGGAAGGGAATTTGGATATGGACGTCGTCAATGGCTCTGGGGATCGTCGGCAGCCCACCCACCTTCTTATAATGGTTAACGGTCTCTTTGGCAGGTTACTCCATTTTCCTATGAGATTCTTTTGGTTGTTGCTATGATTCAAGCAATTGAAATCCCAATTCATCGCTCTATAATGTTTTCTCTTTAGTATTTCATTTGCATTTATGTGGGTACTGTTTCTATATTGGTTTCTGATTTCGTTTACTGAATAGGATAACGGACGACTTATCTTATAATCAATAGTGGGTCGTATTGTTCTACTTCTGGTAGGTATTAGCTCTAGCTGTAGAATGTTGGCCAGCTAAGCCCCAACTATTTTAACAAAGAGTTGGTCCTAAACACTAATTAATAACAAACTATTAATGGGCTTTTCTTTACACTGTAAACAAATCCATGCCATTTGGTATTTACATAGTCGATTGTTATCCTTTTCTGGGTGATGCATTGGCAGTGCCAAAGATTGGAAATATGCAGCGGAGGCGTTTTTGAAGATGTATCCTGAAGATATGGTTGTTCATTGTGAGTCTTTCAATGTGATCTTCTTTTAATTTCAACAATTAAAGTATACACAACGCAAAGGAAGCTTACCCTTTTCATATTTTCTACTGGTTGTCCTGTTTTTGTCTGAATTCCTTTGTGTTCACTTTGTAACAAGCTTCTTTTATGAGTACAATGTCATCAACTATACTTGTGTTATTTGCAGGCAGTAAACGTAATTACTCAACATTGACTCTTGATGGTGTTGATGTGATGGGAGAAAGACTAGCAGAAGAGGTGTTTCATCATTTTCAAAACTAAGTTTATATTTCCATGTTGCCATATAGATTTTGTTTATTTTTCATATGTCAAGCCTTGATTATGTTTTCATTCAGATTTTGTCAGTTATTAGGAGACATTCCAGTGTCCAAAAGATCTCATTCGTATGCCATTCATTAGGCGGACTGATAGCAAGGTATGCCATTGCAAAGCTTTATGAGCAAGATGCACTAAGAGAAGATGTCCAAGTAAATGGAGAGTATGACAAACATGGGCTTAGAGATCAAAGCAAAGAAGAGGAATTTAAAGGAAAAATAGCAGGGCTGGAACCAATTAATTTTATAACATGTGCGACCCCACATCTGGGTTCAAGAGGGCATAAACAGGTGAATGAATAATCAATGGTTTTTTTGCTCTCTTGTTTGATATGCTTAAATGTCACTTCTGCTACTTTGATCATAATTTGACATTGCTGAAAAATTCAAAACTTCTGCACCTTTTCTAGAAAATACACTGTGACCATATATAGGCTATTTTACTATTTATTCTCCCCTTTGATTTGTTCGTGCTCGGCTTCATTGACAATTCGCTAGTGACTACAATCAAACTCTTCATTTTAACGAAGGTTTCGTTCTGAAATAGATTCTTTTGCTGGAGTAAATCTATCTTAGAATTTGAAGGTTATAGTATTCTAGTCATGTATGTGGCTCTTGAATTTATGAAGCACTTTTCACGTTTCAGGTTCCTATGTGTTGTGGCTTTTATGCCCTGGAAAAAGTAGCTGTTGGCACATCATATTTCTTTGGAAGAACTGGGAGGCAATTATTTTTAATTGACAAGGACAGTGGAAAATGCCCCCTTCTTTTTCATATGGCTGGTGATCGTGAAGATCTCAAATTCTTGTGAGTCCACTCTTTTTGAGCTCAAGTTCACAACAATTTCTTGCCTGCTTCTAGAGAAATTCATCTTCACCTGTAATAATATTGTGGTCTTTCTACTGCAGATCTGCTTTGCAGTCGTTTAGACGACGTGTTACCTATGCTAATGCACGTTACGACAGTATCCTGTCATTATTTCTCTACTCCAGTTAAATTACCAATTTGTCCTTGTAGTTTGAAAAAGGTTAGAATTTAGTTCCTATGATTTTAAAATTTAGAATTTCATTCTTATCATTAGATAAAACCTCGTAAACAATCATTACGGTTTGACAAAACATTTATAAATAGTCCCCAGCATTTGATAAATAGTCAAACTATAGAGATGAGTTCTAGCTTTTAAAAACATCGAGACTAAATTCTAAATTTCTCCAAACTAGAAACACCAAATTTGTAATTTAACCCTATTCTTCGATGCTTCCCAATTTTTCAAGTAGGCGCTCTCAAGCATTATATTCCTGTGAAAAGATAGTTGAACTCAAGATGATTGAAAATACTCGAGTCACTGATTCTACTCTCAAGTAAACTCATCAGATTGACAGTTGTATTGCCCTATGTTTATATATAAACCTTAACACTATACACAGACGTCGTTGGATGGTGTACATCTTCTATCAGGCGTCACAAAGAGTTGCCAAAGGTAATTTTGAACATTACCCATGAAAGCTTTCTATGTAGACTTTGAACTACTATCTCACTGGCTTTTACCTTTCAGCTCAAAGGGTTATCAGGCGATAGTAAATATCCATATATTGTAAATGTAGAGATGGCAAAAATTTCCAGTCCTGAGTTATATGTGCCATCAGAGGCCGAAGCCAATGGATACAAGAAAGCTGTTTTGGAAGGTAAGCACTCATTTAATAGTTGCTGTTTTAACTTTCCCAGTCAATTTGACATCAACTGTTCTTGTATTGAGTATCATAAGTTCGTCTCATGTTATTTTATTCATAAGTTTCTTTTGAGATTTCGTGATCATGATTTCCAAAGTTCTTAATTTACAATTTGTTAGTTTGGCCATCTTTAACATTGTAGTATTATTGTGTTAAAATCATTGTAGAAGAAATGATCAAAGGTTTATTGAGCATGAGCTGGGAGCGAGTCGATGTTGACTTTCACAAAAGTAAACAAAGGAATAATGCTCACCTTACCATTCAGGCAAGTTTGCATCCTGCAGCCTAAAATAAAATTTTATCTGGCAAACACAAAACTAGACGTAATTAGCAAGTCATCATCCTTAGATCAGACCTTGTTATCGTTCTCAATTAATAAGTTCAAAGTACATATTTCTATCAGGTTAATAGGTACAAAGACAATTCCGATGGCGCCTGTGTGATCCAACATATGATTGACAAGTTTATCTTGTAGCATGCGACGAATTGAATTCTTGACAATTAATTTCAAGTTCGAATAACAGCCTGGACGGAAAGCATGCTTGCCTACACGCTGGGTTGATCGTTGCTAATAACACCTATACTTGACTGAATTGGCTTGAGCATCAGGTACAGAGTTGTGTTGTTTAGCTCAAAAAGTATTCTTTGTGCATCAAGAATGCAAAGTAGCAGAATATGACTCATGATTCCTACTGTTTGCAAGTGAAGAGGCCTGTTTTGTGTCAAAAGACTTGGAAGCATTGGCATATTCATGAGGTTTGTGGAGTTGAATACAGCAATCAATAGCGGCATTGAATTTATTCTTTTAAAAATTCTTTAGCTCTTGATGCTGTAGCCTCTCCTTTGATAAGAACTGTATTCTCTTTTACTTTGGCACTTTCCGCCAATAAACATTCATTTTGCATAGAATTTAACAAAAAGTGCTACTTGTACGTGCCAGAGAAGCACAAAGAATAGTCTGCAGCAAGTGGAAGTGAAATGAGAATATAATTTTGCTTTCTTTTATCACTGATACCATCCAGCCGCTCTGTGATGATGAAGATTAGTA

mRNA sequence

GCGGCAAGTTATTGGGCGAAGTATAATTTGCCGAATGTTTGAGTCACCCACCAAGAGGGTGGGTGAGGTCGAAATTCCAAACAGTTGAAAATGGAGATGGGCGGTGCTCTGTTTCTTCTACTTTTCTGCGGCCCCATTTGATTTTAGTCTTGATCAACGAGGAGCCGACAGATCCACCACTTCCAAAAGCTTCCCAAATTTACTCTCAACTTCGAAATCGCCACTCCATTTCTTCAAATCGAAGTTGCTGTATGGAAATGTGTGGAAATGAATTGTCAGAGTGGTTGCGGCAGTGAAGTTCGGGAAAATTCTGAATCGGCGGCAGAAGAGAAAATGGTGGTCTCTGCATCTTCGTCTTCCTGCAGATTCAATAGACTATGGCAATGGGTCTTCACTGTAAGCCGGTGGGGTTGCGAGTAGACGGTGTGGCCCTAGTCGTACCATTTTCCCATATAACTACTTCAACCGCTTTCAACGAAAACCTTGTTCGCACGGAAGAATTCAAGGCCTTCATTCTGCTCTGTTTTCAAACACCGACGACGCCACTTTAGCCGACTTTAGCCGACTTCTCTTCCCCTTCTTTCGTTGATTATTCTTGTTTTCTTTGGACTCTGACGTTTTCTTATGGCTTCTGTGGAATTGGGGAAGCCGAGTTATGAGGCTATGAGCCAAGGGGAAACCAGAGGACGATTGGAGAATGAGAAGAGAGAAGAAGCGAGAAATGTGATGAAGAAGAATGGAAATCGTACAGTATCGTTTTCCTTACCTATGCTCGGATGCGGATGTTTCAGAGTACAACGCGATGAGGAAGGGAATTTGGATATGGACGTCGTCAATGGCTCTGGGGATCGTCGGCAGCCCACCCACCTTCTTATAATGGTTAACGGTCTCTTTGGCAGTGCCAAAGATTGGAAATATGCAGCGGAGGCGTTTTTGAAGATGTATCCTGAAGATATGGTTGTTCATTGCAGTAAACGTAATTACTCAACATTGACTCTTGATGGTGTTGATGTGATGGGAGAAAGACTAGCAGAAGAGATTTTGTCAGTTATTAGGAGACATTCCAGTGTCCAAAAGATCTCATTCGTATGCCATTCATTAGGCGGACTGATAGCAAGGTATGCCATTGCAAAGCTTTATGAGCAAGATGCACTAAGAGAAGATGTCCAAGTAAATGGAGAGTATGACAAACATGGGCTTAGAGATCAAAGCAAAGAAGAGGAATTTAAAGGAAAAATAGCAGGGCTGGAACCAATTAATTTTATAACATGTGCGACCCCACATCTGGGTTCAAGAGGGCATAAACAGGTTCCTATGTGTTGTGGCTTTTATGCCCTGGAAAAAGTAGCTGTTGGCACATCATATTTCTTTGGAAGAACTGGGAGGCAATTATTTTTAATTGACAAGGACAGTGGAAAATGCCCCCTTCTTTTTCATATGGCTGGTGATCGTGAAGATCTCAAATTCTTATCTGCTTTGCAGTCGTTTAGACGACGTGTTACCTATGCTAATGCACGTTACGACAACGTCGTTGGATGGTGTACATCTTCTATCAGGCGTCACAAAGAGTTGCCAAAGCTCAAAGGGTTATCAGGCGATAGTAAATATCCATATATTGTAAATGTAGAGATGGCAAAAATTTCCAGTCCTGAGTTATATGTGCCATCAGAGGCCGAAGCCAATGGATACAAGAAAGCTGTTTTGGAAGAAGAAATGATCAAAGGTTTATTGAGCATGAGCTGGGAGCGAGTCGATGTTGACTTTCACAAAAGTAAACAAAGGAATAATGCTCACCTTACCATTCAGGTTAATAGGTACAAAGACAATTCCGATGGCGCCTGTGTGATCCAACATATGATTGACAAGTTTATCTTGTAGCATGCGACGAATTGAATTCTTGACAATTAATTTCAAGTTCGAATAACAGCCTGGACGGAAAGCATGCTTGCCTACACGCTGGGTTGATCGTTGCTAATAACACCTATACTTGACTGAATTGGCTTGAGCATCAGAGGCCTGTTTTGTGTCAAAAGACTTGGAAGCATTGGCATATTCATGAGGTTTGTGGAGTTGAATACAGCAATCAATAGCGGCATTGAATTTATTCTTTTAAAAATTCTTTAGCTCTTGATGCTGTAGCCTCTCCTTTGATAAGAACTGTATTCTCTTTTACTTTGGCACTTTCCGCCAATAAACATTCATTTTGCATAGAATTTAACAAAAAGTGCTACTTGTACGTGCCAGAGAAGCACAAAGAATAGTCTGCAGCAAGTGGAAGTGAAATGAGAATATAATTTTGCTTTCTTTTATCACTGATACCATCCAGCCGCTCTGTGATGATGAAGATTAGTA

Coding sequence (CDS)

ATGGCTTCTGTGGAATTGGGGAAGCCGAGTTATGAGGCTATGAGCCAAGGGGAAACCAGAGGACGATTGGAGAATGAGAAGAGAGAAGAAGCGAGAAATGTGATGAAGAAGAATGGAAATCGTACAGTATCGTTTTCCTTACCTATGCTCGGATGCGGATGTTTCAGAGTACAACGCGATGAGGAAGGGAATTTGGATATGGACGTCGTCAATGGCTCTGGGGATCGTCGGCAGCCCACCCACCTTCTTATAATGGTTAACGGTCTCTTTGGCAGTGCCAAAGATTGGAAATATGCAGCGGAGGCGTTTTTGAAGATGTATCCTGAAGATATGGTTGTTCATTGCAGTAAACGTAATTACTCAACATTGACTCTTGATGGTGTTGATGTGATGGGAGAAAGACTAGCAGAAGAGATTTTGTCAGTTATTAGGAGACATTCCAGTGTCCAAAAGATCTCATTCGTATGCCATTCATTAGGCGGACTGATAGCAAGGTATGCCATTGCAAAGCTTTATGAGCAAGATGCACTAAGAGAAGATGTCCAAGTAAATGGAGAGTATGACAAACATGGGCTTAGAGATCAAAGCAAAGAAGAGGAATTTAAAGGAAAAATAGCAGGGCTGGAACCAATTAATTTTATAACATGTGCGACCCCACATCTGGGTTCAAGAGGGCATAAACAGGTTCCTATGTGTTGTGGCTTTTATGCCCTGGAAAAAGTAGCTGTTGGCACATCATATTTCTTTGGAAGAACTGGGAGGCAATTATTTTTAATTGACAAGGACAGTGGAAAATGCCCCCTTCTTTTTCATATGGCTGGTGATCGTGAAGATCTCAAATTCTTATCTGCTTTGCAGTCGTTTAGACGACGTGTTACCTATGCTAATGCACGTTACGACAACGTCGTTGGATGGTGTACATCTTCTATCAGGCGTCACAAAGAGTTGCCAAAGCTCAAAGGGTTATCAGGCGATAGTAAATATCCATATATTGTAAATGTAGAGATGGCAAAAATTTCCAGTCCTGAGTTATATGTGCCATCAGAGGCCGAAGCCAATGGATACAAGAAAGCTGTTTTGGAAGAAGAAATGATCAAAGGTTTATTGAGCATGAGCTGGGAGCGAGTCGATGTTGACTTTCACAAAAGTAAACAAAGGAATAATGCTCACCTTACCATTCAGGTTAATAGGTACAAAGACAATTCCGATGGCGCCTGTGTGATCCAACATATGATTGACAAGTTTATCTTGTAG

Protein sequence

MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL
Homology
BLAST of CmaCh01G014470 vs. ExPASy Swiss-Prot
Match: P53118 (Putative lipase ROG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ROG1 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.4e-16
Identity = 83/261 (31.80%), Postives = 121/261 (46.36%), Query Frame = 0

Query: 81  HLLIMVNGLFGS-AKDWKYAAEAFLKM---YPEDMVVHCSKRNYSTLTLDGVDVMGERLA 140
           HL+++ +GL  + + D  Y  E   K    YP + +V    R     T  GV  +G RLA
Sbjct: 189 HLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVKYLGTRLA 248

Query: 141 EEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALREDVQVNGEYDKHGLRDQS 200
           E I+  +    S++KISFV HSLGGLI  +AIA +Y                        
Sbjct: 249 EYIIQDL-YDESIRKISFVGHSLGGLIQAFAIAYIY------------------------ 308

Query: 201 KEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEKVAVGTSYFFGRTGRQL 260
             E +      + PINFIT A+P LG        +     A  KV +      G+TG+ L
Sbjct: 309 --EVYPWFFKKVNPINFITLASPLLG--------IVTDNPAYIKVLLSFG-VIGKTGQDL 368

Query: 261 FL-IDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYDNVVGWCTSS---IRR 320
            L  D + GK PLL+ ++G    L  +  L+ F+RR  YANA  D +V   T+S   +  
Sbjct: 369 GLENDVEVGK-PLLYLLSG----LPLIEILRRFKRRTVYANAINDGIVPLYTASLLFLDY 408

Query: 321 HKELPKLKGLSGDSKYPYIVN 334
           +  L +L+ L  +SK   ++N
Sbjct: 429 NDILEQLQKLKENSKKSPLIN 408

BLAST of CmaCh01G014470 vs. ExPASy Swiss-Prot
Match: Q08448 (Lipid droplet phospholipase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LPL1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.0e-15
Identity = 60/237 (25.32%), Postives = 106/237 (44.73%), Query Frame = 0

Query: 81  HLLIMVNGLFGS---AKDWKYAAEAFLKM--YPEDMVVHCSKRNYSTLTLDGVDVMGERL 140
           HL ++++GL+G+    +  +      LK     +DM+    K+N    T DG++++G R 
Sbjct: 6   HLFVLIHGLWGNYTHMESMRTILSTTLKKEDVNDDMIYFLPKQNAMFKTFDGIEIIGYRT 65

Query: 141 AEEILSVIR--RHSSVQKISFVCHSLGGLIARYAIAKLYEQDALREDVQVNGEYDKHGLR 200
             E+   IR  +   + K+S + +S GGL+AR+ I K+                      
Sbjct: 66  LIEVCEFIRDYKDGKITKLSVMGYSQGGLVARFMIGKML--------------------- 125

Query: 201 DQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEKVAVGTSYFFGRTG 260
                 EFK     +EP  FIT ATPHLG   +    +         +    S   G++G
Sbjct: 126 -----TEFKELFEDIEPQLFITMATPHLGVEFYNPTGIAYKSALYSALRTLGSTILGKSG 185

Query: 261 RQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYDNVVGWCTSSI 311
           R++F+ +  +    +L  ++      ++L AL  F+ R+ +AN + D  V + T+ I
Sbjct: 186 REMFIANSSNN---ILVKLSQG----EYLEALSLFKWRIAFANVKNDRTVAFYTAFI 209

BLAST of CmaCh01G014470 vs. ExPASy Swiss-Prot
Match: Q12103 (Putative lipase YDL109C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDL109C PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-13
Identity = 74/259 (28.57%), Postives = 114/259 (44.02%), Query Frame = 0

Query: 75  DRRQPTHLLIMVNGLFGS-AKDWKYAAEAFLKMY---PEDMVVHCSKRNYSTLTLDGVDV 134
           D  Q +HL+I+ +G   + + D +Y  E   K     P + +V       +  T  G+  
Sbjct: 188 DMSQKSHLVILTHGFQSNVSADMEYLMEEIYKAQMNNPNERLVIKGYMKNACETEKGIKF 247

Query: 135 MGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALREDVQVNGEYDKH 194
           +G  LA  I+  +    SV KISF+ HSLGGL   +AI  +                   
Sbjct: 248 LGVGLANYIIDEL-YDDSVGKISFIGHSLGGLTQTFAICYI------------------- 307

Query: 195 GLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEKVAVGTSYFFG 254
               ++K   F  K+   EPINFI+ A+P LG      +      Y    +++G     G
Sbjct: 308 ----KTKYPYFFKKV---EPINFISLASPLLG------IATSTPNYVKMSLSMG---IIG 367

Query: 255 RTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYDNVVGWCTSSI 314
            TG++L L D + G  PLL+ ++ +      +S L  F+RR  YANA  D +V   +SS+
Sbjct: 368 TTGQELGLKDGNYGDKPLLYLLSEE----SLISVLARFKRRTLYANAVNDGIVPLYSSSL 406

Query: 315 RRHKELPKLKGLSGDSKYP 330
                   L+ L G +  P
Sbjct: 428 LFLDYSQLLQKLGGQTTAP 406

BLAST of CmaCh01G014470 vs. ExPASy Swiss-Prot
Match: O14162 (Putative lipase C4A8.10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4A8.10 PE=3 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 2.6e-10
Identity = 66/257 (25.68%), Postives = 105/257 (40.86%), Query Frame = 0

Query: 80  THLLIMVNGLFGS-AKDWKYAAEAFL---KMYPEDMVVHCSKRNYSTLTLDGVDVMGERL 139
           +HL+++ +G+  +   D +Y  E  +   K   E +VV     NY   T  GV  +G+RL
Sbjct: 305 SHLVVLTHGMHSNVGADMEYLKEKLIESSKSVKELVVVRGFTGNYCQ-TEKGVRWLGKRL 364

Query: 140 AEEILSVIRRHSS----VQKISFVCHSLGGLIARYAIAKLYEQDALREDVQVNGEYDKHG 199
            E +L +    S+       IS V HSLGGL+  YA+  ++ +               HG
Sbjct: 365 GEWLLDITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYVHAK--------------THG 424

Query: 200 LRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEKVAVGTSY-FFG 259
              Q+           + P+ F+T ATP LG  G               +    SY   G
Sbjct: 425 AFFQA-----------IHPVFFVTLATPWLGVAGEHP----------SYIGKALSYGIIG 484

Query: 260 RTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYDNVVGWCTSSI 319
           +TG+ L L   +       F +        F  A+  F +R+ +AN   D +V + TS++
Sbjct: 485 KTGQDLSLTPLNHSIESRPFLVLMSDPSTPFFQAVSFFEKRILFANTTNDYIVPFGTSAM 524

Query: 320 RRHKELPKLKGLSGDSK 328
                L K++   G  K
Sbjct: 545 -EVSSLGKVEEAEGSDK 524

BLAST of CmaCh01G014470 vs. ExPASy Swiss-Prot
Match: Q04093 (Putative lipase YDR444W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDR444W PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-09
Identity = 83/356 (23.31%), Postives = 136/356 (38.20%), Query Frame = 0

Query: 78  QPTHLLIMVNGLFGS-AKDWKYAAEAFLKM-YPED------MVVHCSKRNYSTLTLDGVD 137
           +P HL+IM +G+F +   D  Y  +   +M +P D      ++V     N    +  G+ 
Sbjct: 194 KPVHLVIMTHGIFSNIGCDMLYMKDKIEEMTFPMDESINPNIIVRGFMGNVGK-SGHGIH 253

Query: 138 VMGERLAEEILSVI---RRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALREDVQVNGE 197
            +G R+ + +L  +    +   V +ISF+ HSLGG     A+  +               
Sbjct: 254 CLGVRVGKYVLETVDKLNKKYKVDRISFIGHSLGGPTQSMAVRYI--------------- 313

Query: 198 YDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEKVAVGTS 257
                     K  +F   + G++P+NFIT A+P +G  G         FY    + +G  
Sbjct: 314 --------SIKRPDFFDAVKGVKPVNFITLASPFIGVIGD------FPFYLSVPLDMGA- 373

Query: 258 YFFGRTGRQLF-----LIDKDSGKCPLLFHMAGDREDLKFL------SALQSFRRRVTYA 317
              G TGR L      L  KD        +    +  L+ L         +SF+RR  YA
Sbjct: 374 --LGLTGRDLNLKYTPLTSKDGLYADDEVYPEHSKYILEILPQAPAKKVFESFKRRTIYA 433

Query: 318 NARYDNVVGWCTSSI-----RRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELY-VPSE 377
           N   D +V   T+++     R   ++ K++  + +S      + E     SPE     S 
Sbjct: 434 NVMDDGIVPLRTAALLYLDWRSIHKVQKIRKKNKNSP----TSSEFVSSDSPESSGASSP 493

Query: 378 AEANGYKKAVLEEEMIKGLLSMSWERVDVDFHKSK-QRNNAHLTIQVNRYKDNSDG 405
           +  NG     +  E      ++ W       H SK  +     T + N   DN  G
Sbjct: 494 SNENGNNVGEIPAESPNKKATLQWTLPQAVIHGSKINKYKRGQTNEANSDSDNEQG 512

BLAST of CmaCh01G014470 vs. ExPASy TrEMBL
Match: A0A6J1IV98 (putative lipase ROG1 OS=Cucurbita maxima OX=3661 GN=LOC111480282 PE=4 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 2.9e-243
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 1   MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD 60
           MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD
Sbjct: 1   MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD 60

Query: 61  EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY 120
           EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY
Sbjct: 61  EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY 120

Query: 121 STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180
           STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED
Sbjct: 121 STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180

Query: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240
           VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK
Sbjct: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240

Query: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300
           VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD
Sbjct: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300

Query: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360
           NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL
Sbjct: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360

Query: 361 EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL 418
           EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL
Sbjct: 361 EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL 417

BLAST of CmaCh01G014470 vs. ExPASy TrEMBL
Match: A0A6J1FT75 (putative lipase ROG1 OS=Cucurbita moschata OX=3662 GN=LOC111446646 PE=4 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.3e-235
Identity = 404/417 (96.88%), Postives = 409/417 (98.08%), Query Frame = 0

Query: 1   MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD 60
           MASVELGKPSYEA+SQGE RGR ENEKREEARN MKKNGNRTVS SLPMLGCGCFRVQRD
Sbjct: 1   MASVELGKPSYEAVSQGENRGRFENEKREEARNGMKKNGNRTVSSSLPMLGCGCFRVQRD 60

Query: 61  EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY 120
           EEGNLD+DVVNGSG+R QPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPED+VVHCSKRNY
Sbjct: 61  EEGNLDIDVVNGSGERPQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDIVVHCSKRNY 120

Query: 121 STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180
           STLTLDGVDVMGERLAEEILSVIR HSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED
Sbjct: 121 STLTLDGVDVMGERLAEEILSVIRTHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180

Query: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240
           VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK
Sbjct: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240

Query: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300
           VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD
Sbjct: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300

Query: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360
           NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL
Sbjct: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360

Query: 361 EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL 418
           EEEMIKGL S+SWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMID FIL
Sbjct: 361 EEEMIKGLSSVSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDNFIL 417

BLAST of CmaCh01G014470 vs. ExPASy TrEMBL
Match: A0A1S3CRU4 (putative lipase ROG1 OS=Cucumis melo OX=3656 GN=LOC103503619 PE=4 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 4.5e-191
Identity = 332/422 (78.67%), Postives = 366/422 (86.73%), Query Frame = 0

Query: 4   VELGKPSYEAMSQGETRGRLENEKREE--------ARNVMKKNGNRTVSFSLPMLGCGCF 63
           +EL  P+    S+   RG LENEK  E         +   KKNGNR+VSF LP +G GCF
Sbjct: 1   MELENPASRTSSEEGNRGELENEKGGELDLNDTRNGKKKKKKNGNRSVSFYLPKIGFGCF 60

Query: 64  RVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHC 123
           RVQRDEEGN+DM+VVNGSG+RR+P+HLLIMVNGL GSAKDWKYAA+ FLK YPED++VHC
Sbjct: 61  RVQRDEEGNIDMEVVNGSGERRKPSHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVHC 120

Query: 124 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 183
           SKRNYSTLTLDGVDVMG RLAEEIL VI+RH +++KISF+CHSLGGLIARYAIAKLYE  
Sbjct: 121 SKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNLRKISFLCHSLGGLIARYAIAKLYE-- 180

Query: 184 ALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 243
            L+EDVQVNGEY+KH  RD+S E+EF+GKIAGLEPINFITCATPHLGSRGHKQVPMCCGF
Sbjct: 181 -LKEDVQVNGEYNKHEFRDESYEDEFRGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 240

Query: 244 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 303
           Y LEKVAV TSYFFGRTGR LFLIDKDSG CPLLFHMAGDREDLKFLSALQSFRRRVTYA
Sbjct: 241 YVLEKVAVCTSYFFGRTGRHLFLIDKDSGNCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300

Query: 304 NARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY 363
           N RYDNVVGW TSSIR   ELPKLKGLSGDSKYPYIVNVEMAKI +P+LYVPSEAE N +
Sbjct: 301 NVRYDNVVGWSTSSIRHRSELPKLKGLSGDSKYPYIVNVEMAKIQNPQLYVPSEAEVNRF 360

Query: 364 KKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKF 418
           KK+ LEEEMIKGL S+ W+RVDVDFH+SKQRN+AHLTIQVNRY+ NSDGACVIQHMID F
Sbjct: 361 KKSDLEEEMIKGLSSVGWDRVDVDFHRSKQRNDAHLTIQVNRYRVNSDGACVIQHMIDNF 419

BLAST of CmaCh01G014470 vs. ExPASy TrEMBL
Match: A0A0A0LE49 (DUF676 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G894530 PE=4 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.9e-190
Identity = 329/422 (77.96%), Postives = 366/422 (86.73%), Query Frame = 0

Query: 4   VELGKPSYEAMSQGETRGRLENEKREE--------ARNVMKKNGNRTVSFSLPMLGCGCF 63
           +EL  P+ + +S+   RG+LENEK  E         +   KKNGNR+VSF LP +G GCF
Sbjct: 1   MELENPASQTLSEEGNRGKLENEKGGELDLNETRNGKKKKKKNGNRSVSFYLPKIGFGCF 60

Query: 64  RVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHC 123
           RVQRDEEGN+DM+VVNGSG+R++PTHLLIMVNGL GSAKDWKYAA+ FLK YPED++VHC
Sbjct: 61  RVQRDEEGNVDMEVVNGSGERQKPTHLLIMVNGLVGSAKDWKYAAQEFLKTYPEDIIVHC 120

Query: 124 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 183
           SKRNYSTLTLDGVDVMG RLAEEIL VI+RH +V+KISF+CHSLGGLIARYAIAKLYE  
Sbjct: 121 SKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNVRKISFLCHSLGGLIARYAIAKLYE-- 180

Query: 184 ALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 243
            L+EDVQVNGEY+KH  RD+S E+EF+G+IAGLEPINFITCATPHLGSRGH QVPMCCGF
Sbjct: 181 -LKEDVQVNGEYNKHEFRDESYEDEFRGRIAGLEPINFITCATPHLGSRGHNQVPMCCGF 240

Query: 244 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 303
           Y LEKVAV TSYFFGRTGR LFLID DSG CPLLFHMAGDREDLKFLSALQSFRRRVTYA
Sbjct: 241 YVLEKVAVCTSYFFGRTGRHLFLIDNDSGNCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300

Query: 304 NARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY 363
           N RYDNVVGW TSSIRR  ELPK KGLSGDSKYPYIVNVEMAKI +P+LYVPSEAE   +
Sbjct: 301 NVRYDNVVGWSTSSIRRRTELPKRKGLSGDSKYPYIVNVEMAKIQNPQLYVPSEAEVKRF 360

Query: 364 KKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKF 418
           KK+ LEEEMIKGL S+ WERVDVDFH+SKQRN+AHLTIQVNRY+ NSDGACV+QHMID F
Sbjct: 361 KKSELEEEMIKGLSSVGWERVDVDFHRSKQRNDAHLTIQVNRYRVNSDGACVVQHMIDNF 419

BLAST of CmaCh01G014470 vs. ExPASy TrEMBL
Match: A0A6J1IER9 (putative lipase C4A8.10 OS=Cucurbita maxima OX=3661 GN=LOC111476589 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.5e-175
Identity = 305/382 (79.84%), Postives = 333/382 (87.17%), Query Frame = 0

Query: 36  KKNGNRTVSFSLPMLGCGCFRVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKD 95
           KKNG R  SF L  +G GCFRVQ+DEEGN DM+VVNG G+RR+PTHLLIMVNGL GSAKD
Sbjct: 21  KKNGIRMESFYLRKIGFGCFRVQQDEEGNFDMEVVNGDGERRKPTHLLIMVNGLVGSAKD 80

Query: 96  WKYAAEAFLKMYPEDMVVHCSKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFV 155
           W+YAAE FLK YPED++VHCSKRNYSTLTLDGVDVMG RLAEEIL VI+RH +VQKISF+
Sbjct: 81  WEYAAEEFLKSYPEDIIVHCSKRNYSTLTLDGVDVMGGRLAEEILLVIKRHPNVQKISFI 140

Query: 156 CHSLGGLIARYAIAKLYEQDALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFIT 215
           CHSLGGLIARYAIAKLYEQ   +ED QVNGEY+KHG RDQS  +EFK KIAGLEPINFIT
Sbjct: 141 CHSLGGLIARYAIAKLYEQ---KEDAQVNGEYNKHGSRDQSNGKEFKEKIAGLEPINFIT 200

Query: 216 CATPHLGSRGHKQVPMCCGFYALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGD 275
            ATPHLGSRGHKQVPMCCGFYALEKVA+ TSY FGRTG+ LFL+DKDS  CPLL HMAGD
Sbjct: 201 FATPHLGSRGHKQVPMCCGFYALEKVAICTSYLFGRTGKHLFLLDKDSENCPLLLHMAGD 260

Query: 276 REDLKFLSALQSFRRRVTYANARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVE 335
            EDLKFLSALQSFRRRVTYAN RYDNVVGW TSSIRR  ELPKL+G+S D KYPYIVNVE
Sbjct: 261 HEDLKFLSALQSFRRRVTYANKRYDNVVGWSTSSIRRRTELPKLEGVSIDDKYPYIVNVE 320

Query: 336 MAKISSPELYVPSEAEANGYKKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQV 395
            A+ SSP++YVPSEA+ N  KK  +EEEMIK L S+ WERVDVDFH+SKQRNNAHLTIQV
Sbjct: 321 TAEDSSPQIYVPSEAKGNRSKKTEVEEEMIKCLSSVGWERVDVDFHRSKQRNNAHLTIQV 380

Query: 396 NRYKDNSDGACVIQHMIDKFIL 418
           NRY+ NSDGACVIQHMID F+L
Sbjct: 381 NRYRVNSDGACVIQHMIDNFLL 399

BLAST of CmaCh01G014470 vs. NCBI nr
Match: XP_022981006.1 (putative lipase ROG1 [Cucurbita maxima])

HSP 1 Score: 850.5 bits (2196), Expect = 6.1e-243
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 1   MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD 60
           MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD
Sbjct: 1   MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD 60

Query: 61  EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY 120
           EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY
Sbjct: 61  EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY 120

Query: 121 STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180
           STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED
Sbjct: 121 STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180

Query: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240
           VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK
Sbjct: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240

Query: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300
           VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD
Sbjct: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300

Query: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360
           NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL
Sbjct: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360

Query: 361 EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL 418
           EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL
Sbjct: 361 EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL 417

BLAST of CmaCh01G014470 vs. NCBI nr
Match: XP_022941305.1 (putative lipase ROG1 [Cucurbita moschata])

HSP 1 Score: 824.3 bits (2128), Expect = 4.7e-235
Identity = 404/417 (96.88%), Postives = 409/417 (98.08%), Query Frame = 0

Query: 1   MASVELGKPSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRD 60
           MASVELGKPSYEA+SQGE RGR ENEKREEARN MKKNGNRTVS SLPMLGCGCFRVQRD
Sbjct: 1   MASVELGKPSYEAVSQGENRGRFENEKREEARNGMKKNGNRTVSSSLPMLGCGCFRVQRD 60

Query: 61  EEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNY 120
           EEGNLD+DVVNGSG+R QPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPED+VVHCSKRNY
Sbjct: 61  EEGNLDIDVVNGSGERPQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDIVVHCSKRNY 120

Query: 121 STLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180
           STLTLDGVDVMGERLAEEILSVIR HSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED
Sbjct: 121 STLTLDGVDVMGERLAEEILSVIRTHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRED 180

Query: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240
           VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK
Sbjct: 181 VQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEK 240

Query: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300
           VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD
Sbjct: 241 VAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 300

Query: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360
           NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL
Sbjct: 301 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 360

Query: 361 EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKFIL 418
           EEEMIKGL S+SWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMID FIL
Sbjct: 361 EEEMIKGLSSVSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDNFIL 417

BLAST of CmaCh01G014470 vs. NCBI nr
Match: KAG6608103.1 (hypothetical protein SDJN03_01445, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 816.2 bits (2107), Expect = 1.3e-232
Identity = 404/422 (95.73%), Postives = 409/422 (96.92%), Query Frame = 0

Query: 1   MASVELGKPSYEAMSQGETRGRLENEKREE-----ARNVMKKNGNRTVSFSLPMLGCGCF 60
           MASVELGKPSYEA+SQGETRGRLEN KREE      RN MKKNGNRTVSFS PMLGCGCF
Sbjct: 1   MASVELGKPSYEAVSQGETRGRLENGKREETDLNDTRNGMKKNGNRTVSFSSPMLGCGCF 60

Query: 61  RVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHC 120
           RVQRDEEGNLDMDVVNGSG+R QPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPED+VVHC
Sbjct: 61  RVQRDEEGNLDMDVVNGSGERPQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDIVVHC 120

Query: 121 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 180
           SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD
Sbjct: 121 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 180

Query: 181 ALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 240
           AL+EDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF
Sbjct: 181 ALKEDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 240

Query: 241 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300
           YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA
Sbjct: 241 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300

Query: 301 NARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY 360
           NARYDNVVGWCTSSIRRHKELPKLK LSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY
Sbjct: 301 NARYDNVVGWCTSSIRRHKELPKLKNLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY 360

Query: 361 KKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKF 418
           KKAVLEEEMIKGLLS+SWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGA VIQHMID F
Sbjct: 361 KKAVLEEEMIKGLLSVSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGAGVIQHMIDNF 420

BLAST of CmaCh01G014470 vs. NCBI nr
Match: XP_023525017.1 (putative lipase YDL109C [Cucurbita pepo subsp. pepo])

HSP 1 Score: 816.2 bits (2107), Expect = 1.3e-232
Identity = 403/422 (95.50%), Postives = 409/422 (96.92%), Query Frame = 0

Query: 1   MASVELGKPSYEAMSQGETRGRLENEKREE-----ARNVMKKNGNRTVSFSLPMLGCGCF 60
           MASVELGKPSYEAMSQGETR RLEN KREE      RN MKKNGNRTVS SLPMLGCGCF
Sbjct: 1   MASVELGKPSYEAMSQGETRARLENGKREETDLNDTRNGMKKNGNRTVSSSLPMLGCGCF 60

Query: 61  RVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHC 120
           RVQRDEEGNLD+DVVNGSG+R QPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPED+VVHC
Sbjct: 61  RVQRDEEGNLDIDVVNGSGERPQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDIVVHC 120

Query: 121 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 180
           SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD
Sbjct: 121 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 180

Query: 181 ALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 240
           AL+EDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF
Sbjct: 181 ALKEDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 240

Query: 241 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300
           YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA
Sbjct: 241 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300

Query: 301 NARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY 360
           NARYDNVVGWCTSSIRRHKELPKLKGLSGDS+YPYIVNVEMAKISSPELYVPSEAEANGY
Sbjct: 301 NARYDNVVGWCTSSIRRHKELPKLKGLSGDSEYPYIVNVEMAKISSPELYVPSEAEANGY 360

Query: 361 KKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKF 418
           KKAVLEEEMIKGL S+SWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMID F
Sbjct: 361 KKAVLEEEMIKGLSSVSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDNF 420

BLAST of CmaCh01G014470 vs. NCBI nr
Match: KAG7031740.1 (ROG1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 810.8 bits (2093), Expect = 5.3e-231
Identity = 402/422 (95.26%), Postives = 407/422 (96.45%), Query Frame = 0

Query: 1   MASVELGKPSYEAMSQGETRGRLENEKREE-----ARNVMKKNGNRTVSFSLPMLGCGCF 60
           MASVELGKPSYEA+SQGETRGRLEN KREE      RN MKKNGNRTVSFS PMLGCGCF
Sbjct: 1   MASVELGKPSYEAVSQGETRGRLENGKREETDLNDTRNGMKKNGNRTVSFSSPMLGCGCF 60

Query: 61  RVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHC 120
           RVQRDEEGNLDMDVVNGSG+R Q THLLIMVNGLFGSAKDWKYAAEAFLKMYPED+VVHC
Sbjct: 61  RVQRDEEGNLDMDVVNGSGERPQATHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDIVVHC 120

Query: 121 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 180
           SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD
Sbjct: 121 SKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQD 180

Query: 181 ALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 240
           AL+EDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF
Sbjct: 181 ALKEDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGF 240

Query: 241 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300
           YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA
Sbjct: 241 YALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYA 300

Query: 301 NARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY 360
           NARYDNVVGWCTSSIRRHKELPKLK LSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY
Sbjct: 301 NARYDNVVGWCTSSIRRHKELPKLKDLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGY 360

Query: 361 KKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKF 418
           KKAVLEEEM KGLLS+SWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGA VIQHMID F
Sbjct: 361 KKAVLEEEMTKGLLSVSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGAGVIQHMIDNF 420

BLAST of CmaCh01G014470 vs. TAIR 10
Match: AT1G10040.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 7.4e-114
Identity = 209/363 (57.58%), Postives = 261/363 (71.90%), Query Frame = 0

Query: 53  GCFRVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMV 112
           GC R + DE GN+D+  V+  G+R +PTHL++MVNGL GSA++W++AA+  LK YP+D++
Sbjct: 54  GCLRAESDESGNVDL-TVDFPGERTEPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLL 113

Query: 113 VHCSKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLY 172
           VHCSKRN+ST T DGVDVMGERLAEE+ SVI+RH S+QKISFV HSLGGLIARYAI +LY
Sbjct: 114 VHCSKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLY 173

Query: 173 EQDALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMC 232
           EQ++ RE++  N +     + D+   EE K +IAGLEP+ FIT ATPHLGSRGHKQVP+ 
Sbjct: 174 EQES-REELPHNSD----DIGDKCSIEEPKARIAGLEPVYFITSATPHLGSRGHKQVPLF 233

Query: 233 CGFYALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRV 292
            G Y LE++A   S   G+TG+ LFL D D GK PLL  M  D  DLKF+SALQ F+RR+
Sbjct: 234 SGSYTLERLATRMSGCLGKTGKHLFLADSDGGKPPLLLRMVKDSRDLKFISALQCFKRRI 293

Query: 293 TYANARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEA 352
            YAN  +D++VGW TSSIRRH ELPKL+    + KYP+IVNVE    +S      S  +A
Sbjct: 294 AYANTSFDHLVGWSTSSIRRHNELPKLQRGPVNEKYPHIVNVEAPDTASNYEEDHSRTKA 353

Query: 353 NGYKKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMI 412
           + +K   +EEEMI+ L  +SWERVDV F  + QR  AH TIQV     NS GA VIQHMI
Sbjct: 354 DRFKNLDMEEEMIRELTKLSWERVDVSFRGTLQRFLAHNTIQVKTKMINSAGADVIQHMI 410

Query: 413 DKF 416
           D F
Sbjct: 414 DNF 410

BLAST of CmaCh01G014470 vs. TAIR 10
Match: AT1G10040.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 9.4e-109
Identity = 198/349 (56.73%), Postives = 252/349 (72.21%), Query Frame = 0

Query: 53  GCFRVQRDEEGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMV 112
           GC R + DE GN+D+  V+  G+R +PTHL++MVNGL GSA++W++AA+  LK YP+D++
Sbjct: 54  GCLRAESDESGNVDL-TVDFPGERTEPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLL 113

Query: 113 VHCSKRNYSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLY 172
           VHCSKRN+ST T DGVDVMGERLAEE+ SVI+RH S+QKISFV HSLGGLIARYAI +LY
Sbjct: 114 VHCSKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLY 173

Query: 173 EQDALREDVQVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMC 232
           EQ++ RE++  N +     + D+   EE K +IAGLEP+ FIT ATPHLGSRGHKQVP+ 
Sbjct: 174 EQES-REELPHNSD----DIGDKCSIEEPKARIAGLEPVYFITSATPHLGSRGHKQVPLF 233

Query: 233 CGFYALEKVAVGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRV 292
            G Y LE++A   S   G+TG+ LFL D D GK PLL  M  D  DLKF+SALQ F+RR+
Sbjct: 234 SGSYTLERLATRMSGCLGKTGKHLFLADSDGGKPPLLLRMVKDSRDLKFISALQCFKRRI 293

Query: 293 TYANARYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEA 352
            YAN  +D++VGW TSSIRRH ELPKL+    + KYP+IVNVE    +S      S  +A
Sbjct: 294 AYANTSFDHLVGWSTSSIRRHNELPKLQRGPVNEKYPHIVNVEAPDTASNYEEDHSRTKA 353

Query: 353 NGYKKAVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDN 402
           + +K   +EEEMI+ L  +SWERVDV F  + QR  AH TIQ +  KD+
Sbjct: 354 DRFKNLDMEEEMIRELTKLSWERVDVSFRGTLQRFLAHNTIQASENKDD 396

BLAST of CmaCh01G014470 vs. TAIR 10
Match: AT4G25770.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 6.1e-100
Identity = 204/418 (48.80%), Postives = 259/418 (61.96%), Query Frame = 0

Query: 9   PSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRDEEGNLDMD 68
           P +   SQ + R RL  +   + +N+ +          +  LG GCF   R +   L  +
Sbjct: 20  PRFLPPSQNQFRFRLYLQFPAQPKNLCQMEW-------MNRLGIGCFAANRSKR-ELTTE 79

Query: 69  VVNGSGD---------RRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRN 128
           + NG  D           +P HL++MVNG+ GSA DWKYAAE F+K +P+ ++VH S+ N
Sbjct: 80  IDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADWKYAAEQFVKKFPDKVLVHRSESN 139

Query: 129 YSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRE 188
            +TLT DGVD MGERLA E+L V++  S ++KISFV HSLGGL+ARYAI KLYEQ     
Sbjct: 140 SATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVAHSLGGLVARYAIGKLYEQP---- 199

Query: 189 DVQVNGEYDKHGLRDQSKEEEFK-GKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYAL 248
                GE D   L   SKE+  + G+IAGLEP+NFIT ATPHLGSRGH+Q P+ CG   L
Sbjct: 200 -----GEVD--SLDSPSKEKSARGGEIAGLEPMNFITFATPHLGSRGHRQFPILCGLPFL 259

Query: 249 EKVAVGTSYF-FGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANA 308
           E+ A  T++   GRTG+ LFL+D D G  PLL  MA D +DLKF+SAL +F+RRV YAN 
Sbjct: 260 ERTASQTAHLAAGRTGKHLFLVDNDDGNAPLLIRMATDSDDLKFISALNAFKRRVAYANV 319

Query: 309 RYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKK 368
            +D++VGW TSSIRR  ELPK   L+ D  YP+IV VE   + +      S       + 
Sbjct: 320 NFDSMVGWRTSSIRRPNELPKPNLLATDPNYPHIVYVERGNVDNGSCQSTSTVVTE--QD 379

Query: 369 AVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKF 416
             LEEEMI GL  +SWERVDV FH SKQR  AH TIQV  Y  +SDG  V+ HM+D F
Sbjct: 380 TDLEEEMIHGLSQLSWERVDVSFHNSKQRYVAHSTIQVKTYWLHSDGKDVVFHMMDHF 416

BLAST of CmaCh01G014470 vs. TAIR 10
Match: AT4G25770.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 339.3 bits (869), Expect = 4.2e-93
Identity = 191/393 (48.60%), Postives = 243/393 (61.83%), Query Frame = 0

Query: 9   PSYEAMSQGETRGRLENEKREEARNVMKKNGNRTVSFSLPMLGCGCFRVQRDEEGNLDMD 68
           P +   SQ + R RL  +   + +N+ +          +  LG GCF   R +   L  +
Sbjct: 20  PRFLPPSQNQFRFRLYLQFPAQPKNLCQMEW-------MNRLGIGCFAANRSKR-ELTTE 79

Query: 69  VVNGSGD---------RRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRN 128
           + NG  D           +P HL++MVNG+ GSA DWKYAAE F+K +P+ ++VH S+ N
Sbjct: 80  IDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADWKYAAEQFVKKFPDKVLVHRSESN 139

Query: 129 YSTLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALRE 188
            +TLT DGVD MGERLA E+L V++  S ++KISFV HSLGGL+ARYAI KLYEQ     
Sbjct: 140 SATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVAHSLGGLVARYAIGKLYEQP---- 199

Query: 189 DVQVNGEYDKHGLRDQSKEEEFK-GKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYAL 248
                GE D   L   SKE+  + G+IAGLEP+NFIT ATPHLGSRGH+Q P+ CG   L
Sbjct: 200 -----GEVD--SLDSPSKEKSARGGEIAGLEPMNFITFATPHLGSRGHRQFPILCGLPFL 259

Query: 249 EKVAVGTSYF-FGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANA 308
           E+ A  T++   GRTG+ LFL+D D G  PLL  MA D +DLKF+SAL +F+RRV YAN 
Sbjct: 260 ERTASQTAHLAAGRTGKHLFLVDNDDGNAPLLIRMATDSDDLKFISALNAFKRRVAYANV 319

Query: 309 RYDNVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKK 368
            +D++VGW TSSIRR  ELPK   L+ D  YP+IV VE   + +      S       + 
Sbjct: 320 NFDSMVGWRTSSIRRPNELPKPNLLATDPNYPHIVYVERGNVDNGSCQSTSTVVTE--QD 379

Query: 369 AVLEEEMIKGLLSMSWERVDVDFHKSKQRNNAH 391
             LEEEMI GL  +SWERVDV FH SKQR  AH
Sbjct: 380 TDLEEEMIHGLSQLSWERVDVSFHNSKQRYVAH 391

BLAST of CmaCh01G014470 vs. TAIR 10
Match: AT5G51180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 328.9 bits (842), Expect = 5.7e-90
Identity = 183/355 (51.55%), Postives = 236/355 (66.48%), Query Frame = 0

Query: 62  EGNLDMDVVNGSGDRRQPTHLLIMVNGLFGSAKDWKYAAEAFLKMYPEDMVVHCSKRNYS 121
           +G+LD+     S D     HL++MV+G+ GS  DWK+ AE F+K  P+ + VHCS++N S
Sbjct: 17  DGSLDVWSCQNS-DSSSADHLVVMVHGILGSTDDWKFGAEQFVKKMPDKVFVHCSEKNVS 76

Query: 122 TLTLDGVDVMGERLAEEILSVIRRHSSVQKISFVCHSLGGLIARYAIAKLYEQDALREDV 181
            LTLDGVDVMGERLA E+L +I+R  ++ KISFV HSLGGL ARYAI KLY + A +EDV
Sbjct: 77  ALTLDGVDVMGERLAAEVLDIIQRKPNICKISFVAHSLGGLAARYAIGKLY-KPANQEDV 136

Query: 182 QVNGEYDKHGLRDQSKEEEFKGKIAGLEPINFITCATPHLGSRGHKQVPMCCGFYALEKV 241
                  K  + D S+E   KG I GLE +NFIT ATPHLGS G+KQVP   GF ++EKV
Sbjct: 137 -------KDSVADSSQETP-KGTICGLEAMNFITVATPHLGSMGNKQVPFLFGFSSIEKV 196

Query: 242 A-VGTSYFFGRTGRQLFLIDKDSGKCPLLFHMAGDREDLKFLSALQSFRRRVTYANARYD 301
           A +   + F RTGR LFL D++ GK PLL  M  D +D  F+SAL++F+RRV Y+N  +D
Sbjct: 197 AGLIIHWIFKRTGRHLFLKDEEEGKPPLLRRMVEDTDDCHFISALRAFKRRVAYSNVGHD 256

Query: 302 NVVGWCTSSIRRHKELPKLKGLSGDSKYPYIVNVEMAKISSPELYVPSEAEANGYKKAVL 361
           +VVGW T+SIRR  ELPK +  S + KYP+IV  E+ K    E     E  ++      +
Sbjct: 257 HVVGWKTASIRRDSELPKWED-SLNEKYPHIVYEELCKACDMEDIPEGENHSDD-----I 316

Query: 362 EEEMIKGLLSMSWERVDVDFHKSKQRNNAHLTIQVNRYKDNSDGACVIQHMIDKF 416
           EEEMIKGL S+SWE+VDV FH S+QR  AH  IQV     + +GA VI+H+ID F
Sbjct: 317 EEEMIKGLSSVSWEKVDVSFHSSRQRFAAHSVIQVKNEDMHIEGADVIEHIIDHF 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P531181.4e-1631.80Putative lipase ROG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX... [more]
Q084482.0e-1525.32Lipid droplet phospholipase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / ... [more]
Q121031.1e-1328.57Putative lipase YDL109C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c)... [more]
O141622.6e-1025.68Putative lipase C4A8.10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O... [more]
Q040932.8e-0923.31Putative lipase YDR444W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c)... [more]
Match NameE-valueIdentityDescription
A0A6J1IV982.9e-243100.00putative lipase ROG1 OS=Cucurbita maxima OX=3661 GN=LOC111480282 PE=4 SV=1[more]
A0A6J1FT752.3e-23596.88putative lipase ROG1 OS=Cucurbita moschata OX=3662 GN=LOC111446646 PE=4 SV=1[more]
A0A1S3CRU44.5e-19178.67putative lipase ROG1 OS=Cucumis melo OX=3656 GN=LOC103503619 PE=4 SV=1[more]
A0A0A0LE494.9e-19077.96DUF676 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G894530 PE=4... [more]
A0A6J1IER91.5e-17579.84putative lipase C4A8.10 OS=Cucurbita maxima OX=3661 GN=LOC111476589 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022981006.16.1e-243100.00putative lipase ROG1 [Cucurbita maxima][more]
XP_022941305.14.7e-23596.88putative lipase ROG1 [Cucurbita moschata][more]
KAG6608103.11.3e-23295.73hypothetical protein SDJN03_01445, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023525017.11.3e-23295.50putative lipase YDL109C [Cucurbita pepo subsp. pepo][more]
KAG7031740.15.3e-23195.26ROG1 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G10040.17.4e-11457.58alpha/beta-Hydrolases superfamily protein [more]
AT1G10040.29.4e-10956.73alpha/beta-Hydrolases superfamily protein [more]
AT4G25770.16.1e-10048.80alpha/beta-Hydrolases superfamily protein [more]
AT4G25770.24.2e-9348.60alpha/beta-Hydrolases superfamily protein [more]
AT5G51180.15.7e-9051.55alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 75..315
e-value: 3.1E-14
score: 55.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 78..310
IPR007751Domain of unknown function DUF676, lipase-likePFAMPF05057DUF676coord: 77..307
e-value: 3.3E-62
score: 209.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR12482:SF41ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 25..416
IPR044294Lipase-likePANTHERPTHR12482UNCHARACTERIZEDcoord: 25..416

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G014470.1CmaCh01G014470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044255 cellular lipid metabolic process