CmaCh01G014360 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G014360
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncarotenoid 9,10(9',10')-cleavage dioxygenase 1-like
LocationCma_Chr01: 10099857 .. 10105030 (-)
RNA-Seq ExpressionCmaCh01G014360
SyntenyCmaCh01G014360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGAAGCAAAACGAGACAGCCACATGGGACGTTCCGGCATGATGTGGATGTTTCCTGGTTCTTCCATCACTCGCAGCCCTTCCATTTCCCTCGCTATTCATCCCTGCAAATCCAATCTCTCTTCGTCAATGGTGCTCTCTCTCTCTGTATATATATATATATATATACATACACATATACATACACACACATATATATAATTGTTATAAATTAAGGTGTTTATGAGCAGCCATTGATTCCGGGGAAGAAACTGAAACAGCAACAGCAACATGTCCGGGTAGACATTGTTAAGAAGCTAAAAAATGGTTGCATGAAATTGGTGGATGGGTTTGTTGATTGGGTGTTTGAATTTTCGGATCAAACCCTTCACCCAAATCAGGTAATTAATTATTTTGTTGTTCATCATCAACAGTGTTTATGTTAATTAATTGTTGTGTATATCAATTATGAACAGACTAACCTTGCGCCGGTTGAAGAGATCGGAGCGCCTGTTGCTGTCACCGATATCCAAGGCACACTCCCCCAACAATTTCCCCAAGGAATCTTCCTAAGAAATGGTAAAGAATTTACTTTATAAATAATCATTTTCCTTTTCTTTAATTTTAATTCCTATGATTTCCAAATATTGGAATTTTATTTTTTTATTAAATAATATAAATTTTTTAAATTGAATTTGGTTCAAGGTAGTAAGATGGTCTATTTAAATTTAAATTTTTATTTTTATTAAAAAAAAAATAGTTAGTTCATAAAAACATACATATATTTATTTATTTTTAATAGTCATTTATTTAAATTTTTATTCTTATTAAAAGAAAATGGTAAGTTTATAAAACATCTATTTATTTATTTTTAACCGTCATTCATTTCAATTTTTATTTTTATTAACGTTAATTCATAAAACACCTATTTATTTATTTTTAACTGTCATTTATTTAAATTTTTTATTTTTATTAAAAGAAAATTGTTAGTTCATAAAATATCTATTTATTTATTTTTAACAGTCATTTATTTAAAATTTTATTTTTATTAAAAGAAAATAGTTAATTAGTAAATATCTATTTATTTATTTTTAACCATTATTTATTTAAATTTGTTATTTTTATTAAAAGAAATGACAGTTATTTAAATTTTTATTTTTATTAAAAGAAAATAGTAAAACATCTATTTATTTATTTTTTAAATATTTAATTAAAATTTGTATAAGTCAAATATTATTTTAAAAATTTGTTGGCAGAAAATACTCCAACAATATTTATGAATTATTATTATTATTATATATATATATATAGAATTCTATACCTAAAACGATATTTTTTTAAAATTAGATTCAACTGGATACGAGTTTCAGAAGGATCTGCACCAAACAAAACCATTTTTTTTGTTTGAACTTAAATAAGCTCATAACCTAACCCAAATAGTCTCGATTGGGTTGGGTTGATCGAGTATTTTGAAAAATTTTAAACACGTATCTACAGGACCGAATCCATTGTTTGGGGGACTAAAATCGACCCGATCGATGTTCGGAAGGTCGAGTTTCATATGGGTGGAAGGGGAAGGAATGCTTCACGCCATGTACTTCAAGAAGAACATGGATGGCAAATGGCATCTGTCGTACAACAATAGATATGTCCAAACCGAATCATTCCGGTTTGAAAAACATGTAAAAAACAGGCCATCGTTCCTTCCTGCAGTGGAAGGCGACGCTGTGGCCATTGTGGCTGCCTTCCTTCTCAACTGGGTCTGCCCATTTTCACTTCACTTTAGGCCTTTCATGTCCACAATAACTAAGACATTGACCAAGTGGCTATTAACTTGACAGCTTAGGTTTGGTGAAGTGAACAAAGACATGAGCAACACCAACGTGATTGAGCACTCTGGCAAGTTTTACTCCGTTGCTGAAAACTCTATGCCCCAACAGATTGATATTATGAGCTTGCAAAGCTTGGGAGTTTGGGATGTTTGTCGTGCTTGGAATCGACCCTTTACTAGTCACCCTAAGGTTCGTCTTCAAATCTCTTTACTCATGTCTACGAGACGGGACAGTGAAATTTGCCTCATCTATGTTGTGTGAATAGGGTGGACATTCTTCGAGAACAGATGGCCATGACACGAAAAGATGTTGATGACACGTGAGTGGATCAATCGAACGAAAATTGGCCATGACCAAACCCATTTTTGGGTATGGTTGCTGATCGTACGTGTTGGAATTATCGAAGTTGAACATTCCTCACAACAATAGCGAAAAAAAATCGATTATGGCTCAGTTTTTTCGCGTTTGTGCTTCAAATAGCCTAATTAGGGTTTTCGAATTATGATTTCAGTTTTAAGCCCATACATACAGTACTTCGGGCTCTTTTCCTTCCAAATTCAAGTCCTAGTATTGTTTCGTAAAGGAATATTAGGATATTGTAGAGAAAAATAGCTTTTCAGAATTGCCCCTAAGACACGAACAAAAAGTCGTCAACGGTACCTGACGTCTAAATATAAAAATTGGGTGGGGCAAGGAGTAGAACGAGGTCAAAGACATGGATGGGAATGTAATACCTGTCGAGAAGAAATTTCTTCTCGTTTTATCTTTCATTTCTTTTAGGTCGGGATCGGGGTCAGGGTCGGAGATGCGAATGAGAATGTAATCCCTCTCATTGCCTTGATTTGTTAATATTTCTCACTTAAATGGGAATTTCCCGCCCAATTTGAGTGGGTTCTCATAACATTCTTCTTCTCTCCCCATCGCAGAAAGCTCCGGACACCGGCGAACTGGTCATCATGGGCATCGCTCCAACCAAACCCTACATGGAAATTGGAATTATTTCCGGTCAATTTTAGTTTCACTACGACGTTCTTCCGCAAACAATTTCTCAATTTCTACTCACTAAACTCTTGTTTTTGTTTATGTAATCGCAGAAGACGGCAAGCGAATGGTTCATAAAGTGGACGTCAAGCTGAGTAGAAGTAGCCTCACCCACGAGATCGGCGTTACACGGAGGTAAACTCACCTGCTTTGCCTTTTAATTTCCTCATAAACAGAGTGTTTTATTGTGTTATGAAATCGGAATTCAGGTTCAATGTGATATTGGATTACCCCGTTACCATCGACTTGAACCGATTAATCAGAGGCGGATCGTACGTTTTTCTTCCTCTGAATCCAATTCGTCTCTGTTTTTAAGCGCAAATTAGGTTCCGAATTCTGAAATTCAAACAATCGTTGTGTTGTAGATTGATAAAATTCGAGAAAGAAGGGTACGCCAAAATCGGAGTGATGCCTCGTTATGGAGACGCCGATTCGATTCAATGGTTTGAAGTGAAACCTAATTGCACTATGCATCTATTCAACTGCTTTGAACACCAAAATGAGGTTAATCATATTACCAAACTCAGAAAAAGTTCAATATTACTGATTTGAGTTGCTGAAGAACAATATTATTAGGTTGTGGTGTGGGGATGTAGAGCTACTGATTCAATTATACCTGGACCTGAAAAGGGGCTCAACAAATTTGAGTGGTTCTCTCAAAGATTCAAACCAGTTCATGTAACTGATGAACAGAACACTGAAGATGGGTCGTTGTTGTCTTGTGCTTACGAATGGAGGCTCAACCTCAAAACAGGGGAGGCTCGAGAGAGATTTCTAACTGGAAATCAATTTTCCATGGATTTTCCCTTCATAAACTCAGCCTTCACTGGTTTGAAAAACAAATATGGATACGCTCAGGTTCTTGACTCTGTCGCTAGCTCTAACTCAGGTACTATTTATCAACAAATTTCAATGCTTAGTAGCCACTCAAGAGACTAGACTTAGATGATGGGTGTGTAAGTTTTGACGAAGTAAGGGAAAAATAACGAGGGATACTACCTTCATTGGTAAGAGTTTACTATAAAAGTAAACGATATTATAGCGCTGCGGAGGTCTATTGTCCCTAACAAGTGGTATAGAAGTCAGGTGTTTAACTTAGTCATGTCAATAGAATTCTTGAGTGTCGAACAAAAAAAGCTGTGAGTATCGAAGTTGTAGTCATAATGAAGAACCGTGATAGAGCTCTATTGGATTGACAGTGTACTCCAAAGAAGTTGACTTGGATAGTGCTGGAATGAATAGATGACTATTTTGTTCGAAGAGAGGATTGTTGGAGTCGTACCTTGTCTAAATAAAAGAAAAATCATGAGTATATAAATAAAAACACTGTCTTCATTGGTATGAGGCATTGCAGGTTCCCATAAATCAAAGTCATTAGAGCTTATGCTCAAACTTGATAATATCATACTATTGTGGAAATTCGTGGCCCTACTATCTATTTGGTCTTAAATTGTCTCATAAACTTTGAAACTATCCCGTACATCATTACTAAACTTGGTAGTTGGTTTGGTTTGGTGTAATAATGTTGTTTGATTTAGGGATATTTAAATTTGGAGGCCTGGCGAAGCTACATTTTGAAGAGCCTCAAAGCGGTGAAGTTTCATTGGTAATTCCAACTCCCAATTACGAAAAACTCACCATCCTGCATAGTTTGTGTACATCTTTTAACTCCATGAAGGGTCCATTACAGCTAAAAGATTCAGAAGAAGAGCCAATAAAAGTGGAGTACCATATGCTTGAGAACAACTCCTTTTGCACCGGAGCTTCCTTTGTGCCCAGAGAAGATAGTCTCCAAGAAGATGATGGTTGGATTATCGCCCATGTTCATAATGAGATCACCAAAACGTCTCAGGTTAATTCCACTTCTTAACAGCTAACTCATTAATTTTATTGCTTAATCAAGTTCTTAATCTCTCTCTTTTATATGGGTTTTTTTTTCTGTCCATATCAGGTATATATAGTTGATGCAAAAAGGTTCAGCGACGAGCCAGTAGCCAAAATCACACTGCCACACAGAGTTCCCTATGGATTCCATGGTGCTTTCATGCCAATCTCATTCAACAAATAATGTATTGGGCAATAAGTTTCTATTCAGATAAACGGTAACCATCTTGGTTATGGTCCCTATTTCTATTGTTTAGGTGGAACTAGTAATTTATGTGATTACCTCAGTTCGAGCATAACTAGATAGATTAGGATCGTGTTAGTTTAGTCCCTATTTTAGTTGTTTAGGTGCCTGTAGCAACTTATGTGATCGTGTCAGTTCGAGCATAATTAGATATCATAATCAAACATAATAGCAAAGCAACCCCTTAGTTTAATA

mRNA sequence

CAGAAGCAAAACGAGACAGCCACATGGGACGTTCCGGCATGATGTGGATGTTTCCTGGTTCTTCCATCACTCGCAGCCCTTCCATTTCCCTCGCTATTCATCCCTGCAAATCCAATCTCTCTTCGTCAATGCCATTGATTCCGGGGAAGAAACTGAAACAGCAACAGCAACATGTCCGGGTAGACATTGTTAAGAAGCTAAAAAATGGTTGCATGAAATTGGTGGATGGGTTTGTTGATTGGGTGTTTGAATTTTCGGATCAAACCCTTCACCCAAATCAGACTAACCTTGCGCCGGTTGAAGAGATCGGAGCGCCTGTTGCTGTCACCGATATCCAAGGCACACTCCCCCAACAATTTCCCCAAGGAATCTTCCTAAGAAATGGACCGAATCCATTGTTTGGGGGACTAAAATCGACCCGATCGATGTTCGGAAGGTCGAGTTTCATATGGGTGGAAGGGGAAGGAATGCTTCACGCCATGTACTTCAAGAAGAACATGGATGGCAAATGGCATCTGTCGTACAACAATAGATATGTCCAAACCGAATCATTCCGGTTTGAAAAACATGTAAAAAACAGGCCATCGTTCCTTCCTGCAGTGGAAGGCGACGCTGTGGCCATTGTGGCTGCCTTCCTTCTCAACTGGCTTAGGTTTGGTGAAGTGAACAAAGACATGAGCAACACCAACGTGATTGAGCACTCTGGCAAGTTTTACTCCGTTGCTGAAAACTCTATGCCCCAACAGATTGATATTATGAGCTTGCAAAGCTTGGGAGTTTGGGATGTTTGTCGTGCTTGGAATCGACCCTTTACTAGTCACCCTAAGAAAGCTCCGGACACCGGCGAACTGGTCATCATGGGCATCGCTCCAACCAAACCCTACATGGAAATTGGAATTATTTCCGAAGACGGCAAGCGAATGGTTCATAAAGTGGACGTCAAGCTGAGTAGAAGTAGCCTCACCCACGAGATCGGCGTTACACGGAGGTTCAATGTGATATTGGATTACCCCGTTACCATCGACTTGAACCGATTAATCAGAGGCGGATCATTGATAAAATTCGAGAAAGAAGGGTACGCCAAAATCGGAGTGATGCCTCGTTATGGAGACGCCGATTCGATTCAATGGTTTGAAGTGAAACCTAATTGCACTATGCATCTATTCAACTGCTTTGAACACCAAAATGAGGTTGTGGTGTGGGGATGTAGAGCTACTGATTCAATTATACCTGGACCTGAAAAGGGGCTCAACAAATTTGAGTGGTTCTCTCAAAGATTCAAACCAGTTCATGTAACTGATGAACAGAACACTGAAGATGGGTCGTTGTTGTCTTGTGCTTACGAATGGAGGCTCAACCTCAAAACAGGGGAGGCTCGAGAGAGATTTCTAACTGGAAATCAATTTTCCATGGATTTTCCCTTCATAAACTCAGCCTTCACTGGTTTGAAAAACAAATATGGATACGCTCAGGTTCTTGACTCTGTCGCTAGCTCTAACTCAGGGATATTTAAATTTGGAGGCCTGGCGAAGCTACATTTTGAAGAGCCTCAAAGCGGTGAAGTTTCATTGCTAAAAGATTCAGAAGAAGAGCCAATAAAAGTGGAGTACCATATGCTTGAGAACAACTCCTTTTGCACCGGAGCTTCCTTTGTGCCCAGAGAAGATAGTCTCCAAGAAGATGATGGTTGGATTATCGCCCATGTTCATAATGAGATCACCAAAACGTCTCAGGTATATATAGTTGATGCAAAAAGGTTCAGCGACGAGCCAGTAGCCAAAATCACACTGCCACACAGAGTTCCCTATGGATTCCATGGTGCTTTCATGCCAATCTCATTCAACAAATAATGTATTGGGCAATAAGTTTCTATTCAGATAAACGGTAACCATCTTGGTTATGGTCCCTATTTCTATTGTTTAGGTGGAACTAGTAATTTATGTGATTACCTCAGTTCGAGCATAACTAGATAGATTAGGATCGTGTTAGTTTAGTCCCTATTTTAGTTGTTTAGGTGCCTGTAGCAACTTATGTGATCGTGTCAGTTCGAGCATAATTAGATATCATAATCAAACATAATAGCAAAGCAACCCCTTAGTTTAATA

Coding sequence (CDS)

ATGGGACGTTCCGGCATGATGTGGATGTTTCCTGGTTCTTCCATCACTCGCAGCCCTTCCATTTCCCTCGCTATTCATCCCTGCAAATCCAATCTCTCTTCGTCAATGCCATTGATTCCGGGGAAGAAACTGAAACAGCAACAGCAACATGTCCGGGTAGACATTGTTAAGAAGCTAAAAAATGGTTGCATGAAATTGGTGGATGGGTTTGTTGATTGGGTGTTTGAATTTTCGGATCAAACCCTTCACCCAAATCAGACTAACCTTGCGCCGGTTGAAGAGATCGGAGCGCCTGTTGCTGTCACCGATATCCAAGGCACACTCCCCCAACAATTTCCCCAAGGAATCTTCCTAAGAAATGGACCGAATCCATTGTTTGGGGGACTAAAATCGACCCGATCGATGTTCGGAAGGTCGAGTTTCATATGGGTGGAAGGGGAAGGAATGCTTCACGCCATGTACTTCAAGAAGAACATGGATGGCAAATGGCATCTGTCGTACAACAATAGATATGTCCAAACCGAATCATTCCGGTTTGAAAAACATGTAAAAAACAGGCCATCGTTCCTTCCTGCAGTGGAAGGCGACGCTGTGGCCATTGTGGCTGCCTTCCTTCTCAACTGGCTTAGGTTTGGTGAAGTGAACAAAGACATGAGCAACACCAACGTGATTGAGCACTCTGGCAAGTTTTACTCCGTTGCTGAAAACTCTATGCCCCAACAGATTGATATTATGAGCTTGCAAAGCTTGGGAGTTTGGGATGTTTGTCGTGCTTGGAATCGACCCTTTACTAGTCACCCTAAGAAAGCTCCGGACACCGGCGAACTGGTCATCATGGGCATCGCTCCAACCAAACCCTACATGGAAATTGGAATTATTTCCGAAGACGGCAAGCGAATGGTTCATAAAGTGGACGTCAAGCTGAGTAGAAGTAGCCTCACCCACGAGATCGGCGTTACACGGAGGTTCAATGTGATATTGGATTACCCCGTTACCATCGACTTGAACCGATTAATCAGAGGCGGATCATTGATAAAATTCGAGAAAGAAGGGTACGCCAAAATCGGAGTGATGCCTCGTTATGGAGACGCCGATTCGATTCAATGGTTTGAAGTGAAACCTAATTGCACTATGCATCTATTCAACTGCTTTGAACACCAAAATGAGGTTGTGGTGTGGGGATGTAGAGCTACTGATTCAATTATACCTGGACCTGAAAAGGGGCTCAACAAATTTGAGTGGTTCTCTCAAAGATTCAAACCAGTTCATGTAACTGATGAACAGAACACTGAAGATGGGTCGTTGTTGTCTTGTGCTTACGAATGGAGGCTCAACCTCAAAACAGGGGAGGCTCGAGAGAGATTTCTAACTGGAAATCAATTTTCCATGGATTTTCCCTTCATAAACTCAGCCTTCACTGGTTTGAAAAACAAATATGGATACGCTCAGGTTCTTGACTCTGTCGCTAGCTCTAACTCAGGGATATTTAAATTTGGAGGCCTGGCGAAGCTACATTTTGAAGAGCCTCAAAGCGGTGAAGTTTCATTGCTAAAAGATTCAGAAGAAGAGCCAATAAAAGTGGAGTACCATATGCTTGAGAACAACTCCTTTTGCACCGGAGCTTCCTTTGTGCCCAGAGAAGATAGTCTCCAAGAAGATGATGGTTGGATTATCGCCCATGTTCATAATGAGATCACCAAAACGTCTCAGGTATATATAGTTGATGCAAAAAGGTTCAGCGACGAGCCAGTAGCCAAAATCACACTGCCACACAGAGTTCCCTATGGATTCCATGGTGCTTTCATGCCAATCTCATTCAACAAATAA

Protein sequence

MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQQIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPISFNK
Homology
BLAST of CmaCh01G014360 vs. ExPASy Swiss-Prot
Match: Q8LP17 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum OX=3888 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.4e-67
Identity = 186/582 (31.96%), Postives = 294/582 (50.52%), Query Frame = 0

Query: 45  KQQQQHVRVDIVKKLKNG----CMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVA 104
           ++++  V +++  K  NG     + L++  +  +F  S    H    N APV++   PV 
Sbjct: 4   EKKENGVILEVEPKPSNGFTSKAVDLLEKIIVKLFYDSSLPHHWLSGNFAPVKDETPPVK 63

Query: 105 VTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMD 164
              +QG LP     G F+R GPNP F  +         + + W +G+GM+H +  K   D
Sbjct: 64  DLTVQGHLPDCL-NGEFVRVGPNPKFSPV---------AGYHWFDGDGMIHGLRIK---D 123

Query: 165 GKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNW------LRFGEV 224
           GK   SY +R+V+T  F+ E++  N   F+    GD   +    ++N       L+  +V
Sbjct: 124 GK--ASYVSRFVKTSRFKQEEYF-NGSKFMKI--GDLKGLFGLLMVNMQMLRAKLKILDV 183

Query: 225 N--KDMSNTNVIEHSGKFYSVAENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKK 284
           +     +NT ++ H  K  +++E   P  I +     LQ+LG+ D  +     FT+HPK 
Sbjct: 184 SYGHGTANTALVYHHQKLLALSEGDKPYAIKVFEDGDLQTLGMLDYDKRLGHNFTAHPKV 243

Query: 285 APDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDY 344
            P TGE+   G + T PY+   +IS+DG  M   V + +S   + H+  +T  +++ +D 
Sbjct: 244 DPFTGEMFTFGYSHTAPYVTYRVISKDG-FMQDPVPITISDPVMMHDFAITENYSIFMDL 303

Query: 345 PVTIDLNRLIRGGSLI-KFEKEGYAKIGVMPRYGDADS-IQWFEVKPNC-TMHLFNCFEH 404
           P+      +++  +LI  F+    A+ GV+PRY   D  I+WFE+ PNC   H  N +E 
Sbjct: 304 PLYFRPKEMVKNKTLIFSFDSTKKARFGVLPRYAKDDKHIRWFEL-PNCFIFHNANAWEE 363

Query: 405 QNEVVVWGCRATD---SIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWR 464
           ++E+V+  CR  +    ++ G  K   K + FS                       YE R
Sbjct: 364 EDEIVLITCRLENPNLDVVGGAVK--EKLDNFSNEL--------------------YEMR 423

Query: 465 LNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAK 524
            N+KTGEA ++ L+ +  ++DFP +N ++TG K +Y Y   LDS+A   +GI KF     
Sbjct: 424 FNMKTGEASQKKLSAS--TVDFPRVNESYTGRKQRYVYGTTLDSIAKV-TGIIKF----D 483

Query: 525 LHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVPR---EDSLQEDDGWIIAH 584
           LH  EP SG+  L      + +    + L    F + A +VPR    DS +EDDG++I  
Sbjct: 484 LH-AEPDSGKTKLEVGGNVQGL----YDLGPGRFGSEAVYVPRVPGTDS-EEDDGYLIFF 530

Query: 585 VHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFM 603
           VH+E T  S V+++DAKR S EPVA + LP RVPYGFH  F+
Sbjct: 544 VHDENTGKSFVHVIDAKRMSAEPVAVVELPQRVPYGFHAFFV 530

BLAST of CmaCh01G014360 vs. ExPASy Swiss-Prot
Match: Q84KG5 (Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus OX=82528 GN=CCD PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.3e-66
Identity = 182/565 (32.21%), Postives = 285/565 (50.44%), Query Frame = 0

Query: 57  KKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGI 116
           K L +  + L++  V ++F    +  H    N APV +   P     ++G LP+    G 
Sbjct: 24  KGLVSSAVDLIEKAVVYLFHDKSKPCHYLSGNFAPVVDETPPCPDLPVRGHLPECL-NGE 83

Query: 117 FLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTES 176
           F+R GPNP F  +         + + W +G+GM+H M  K   DGK   +Y +RYV+T  
Sbjct: 84  FVRVGPNPKFMPV---------AGYHWFDGDGMIHGMRIK---DGK--ATYASRYVKTSR 143

Query: 177 FRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNW------LRFGEVNKDM--SNTNVIEHSG 236
            + E++ +  P F+    GD       F++        L+  +V+  +   NT +I H G
Sbjct: 144 LKQEEYFEG-PKFMKI--GDLKGFFGLFMVQMQLLRAKLKVIDVSYGVGTGNTALIYHHG 203

Query: 237 KFYSVAENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTK 296
           K  +++E   P  + ++    LQ+LG+ D  +  +  FT+HPK  P T E+   G A T 
Sbjct: 204 KLLALSEADKPYVVKVLEDGDLQTLGLLDYDKRLSHSFTAHPKVDPFTDEMFTFGYAHTP 263

Query: 297 PYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLI 356
           PY+   +IS+DG  M   V + +  S + H+  +T  +++ +D P+      +++GG LI
Sbjct: 264 PYVTYRVISKDGV-MRDPVPITIPASVMMHDFAITENYSIFMDLPLYFQPKEMVKGGKLI 323

Query: 357 -KFEKEGYAKIGVMPRYGDADS-IQWFEVKPNC-TMHLFNCFEHQNEVVVWGCRATD--- 416
             F+    A+ GV+PRY   DS I+WFE+ PNC   H  N +E  +EVV+  CR  +   
Sbjct: 324 FSFDATKKARFGVLPRYAKDDSLIRWFEL-PNCFIFHNANAWEEGDEVVLITCRLENPDL 383

Query: 417 SIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGN 476
            ++ G  K   K E F                        YE R N+KTG A ++ L+ +
Sbjct: 384 DMVNGAVK--EKLENFKNEL--------------------YEMRFNMKTGAASQKQLSVS 443

Query: 477 QFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKD 536
             ++DFP IN ++T  K +Y Y  +LD++ +   GI KF     LH  EP++G+  L   
Sbjct: 444 --AVDFPRINESYTTRKQRYVYGTILDNI-TKVKGIIKF----DLH-AEPEAGKKKLEVG 503

Query: 537 SEEEPIKVEYHMLENNSFCTGASFVPREDSL--QEDDGWIIAHVHNEITKTSQVYIVDAK 596
              + I      L    + + A FVPRE  +  +EDDG++I  VH+E T  S+V ++DAK
Sbjct: 504 GNVQGI----FDLGPGRYGSEAVFVPRERGIKSEEDDGYLIFFVHDENTGKSEVNVIDAK 534

Query: 597 RFSDEPVAKITLPHRVPYGFHGAFM 603
             S EPVA + LP+RVPYGFH  F+
Sbjct: 564 TMSAEPVAVVELPNRVPYGFHAFFV 534

BLAST of CmaCh01G014360 vs. ExPASy Swiss-Prot
Match: C3VEQ4 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Oncidium hybrid cultivar OX=141207 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.0e-63
Identity = 182/580 (31.38%), Postives = 283/580 (48.79%), Query Frame = 0

Query: 41  GKKLKQQQQHVRVDIVKKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVA 100
           GKK  +    V+    K + +  + L++  + +    S + LH    N APV +   P A
Sbjct: 25  GKKSHRSVVAVQPKPRKGILSSTIDLIEKVIVYFAHDSSKPLHYLTGNFAPVRDETPPFA 84

Query: 101 VTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMD 160
              + G+LP     G F+R GPNP F  +         + + W +G+GM+H +  K   D
Sbjct: 85  ALTVHGSLPVCL-NGEFVRVGPNPKFSPV---------AGYHWFDGDGMIHGLRIK---D 144

Query: 161 GKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNW------LRFGEV 220
           GK   +Y +RYV+T     E++ +    F+    GD   +   F++        L+  +V
Sbjct: 145 GK--ATYVSRYVKTSKLMQEEYFEG-AKFMKI--GDLKGLFGLFMVQMELLRAKLKILDV 204

Query: 221 N--KDMSNTNVIEHSGKFYSVAENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKK 280
           +  +  +NT +I H GK  +++E   P  I ++    LQ+LG+ D  +     FT+HPK 
Sbjct: 205 SYGRSTANTAMIYHHGKLLALSEGDKPYVIKVLEDGDLQTLGLLDYNKRLAHSFTAHPKV 264

Query: 281 APDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDY 340
            P T E+   G + T PY+   +I++DG  M++ V + +    + H+  +T  + + LD 
Sbjct: 265 DPFTDEMFTFGYSHTSPYITYRVITKDGV-MLNPVPITIPEPIMMHDFAITENYAIFLDL 324

Query: 341 PVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYG-DADSIQWFEVKPNC-TMHLFNCFEHQ 400
           P+       I+G  +  F+    AK GV+PRY  D   I+WF++ PNC   H  N +E  
Sbjct: 325 PLYFRPKETIKGKLIFTFDPTKKAKFGVLPRYAKDEQLIRWFDL-PNCFIFHNANAWEDG 384

Query: 401 NEVVVWGCRATD---SIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRL 460
           +EVV+  CR  +    ++ G  K   K E FS +                     YE R 
Sbjct: 385 DEVVLITCRLKNIDLDLVNGAVK--EKLENFSNQL--------------------YEMRF 444

Query: 461 NLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKL 520
           N+KTG A ++ L+     +DFP IN  +TG K +Y Y     ++   N GI KF     L
Sbjct: 445 NMKTGAATQKELSAPM--VDFPRINENYTGRKQRYVYCTSFANIDKVN-GIVKF----DL 504

Query: 521 HFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVPRE--DSLQEDDGWIIAHVH 580
           H  EP++G+    K  E     +    L    F + A FVPRE    ++EDDG++I  V+
Sbjct: 505 H-AEPETGK----KVLEVGGNVLGIFDLGPGRFGSEAVFVPREAGTRVEEDDGYLIFFVY 550

Query: 581 NEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFM 603
           +E T  S+VY++DAK  S EPVA + LP RVPYGFH  F+
Sbjct: 565 DETTGESKVYVIDAKTMSPEPVAVVDLPCRVPYGFHAFFV 550

BLAST of CmaCh01G014360 vs. ExPASy Swiss-Prot
Match: O65572 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CCD1 PE=1 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 3.8e-63
Identity = 177/563 (31.44%), Postives = 273/563 (48.49%), Query Frame = 0

Query: 57  KKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGI 116
           K   +  + L++  V  +   +   LH    N AP+ +   PV    + G LP+    G 
Sbjct: 20  KGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVHGFLPECL-NGE 79

Query: 117 FLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTES 176
           F+R GPNP F  +         + + W +G+GM+H +  K   DGK   +Y +RYV+T  
Sbjct: 80  FVRVGPNPKFDAV---------AGYHWFDGDGMIHGVRIK---DGK--ATYVSRYVKTSR 139

Query: 177 FRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRF--------GEVNKDMSNTNVIEHSG 236
            + E+       F+    GD        ++N  +                +NT ++ H G
Sbjct: 140 LKQEEFF-GAAKFMKI--GDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHG 199

Query: 237 KFYSVAENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTK 296
           K  ++ E   P  I ++    LQ+LG+ D  +     FT+HPK  P TGE+   G + T 
Sbjct: 200 KLLALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTP 259

Query: 297 PYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLI 356
           PY+   +IS+DG  M   V + +S   + H+  +T  + + +D P+      +++   +I
Sbjct: 260 PYLTYRVISKDG-IMHDPVPITISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMI 319

Query: 357 -KFEKEGYAKIGVMPRYG-DADSIQWFEVKPNC-TMHLFNCFEHQNEVVVWGCRATD--- 416
             F+    A+ GV+PRY  D   I+WFE+ PNC   H  N +E ++EVV+  CR  +   
Sbjct: 320 YSFDPTKKARFGVLPRYAKDELMIRWFEL-PNCFIFHNANAWEEEDEVVLITCRLENPDL 379

Query: 417 SIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGN 476
            ++ G  K   K E F                        YE R N+KTG A ++ L+ +
Sbjct: 380 DMVSGKVK--EKLENFGNEL--------------------YEMRFNMKTGSASQKKLSAS 439

Query: 477 QFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKD 536
             ++DFP IN  +TG K +Y Y  +LDS+A   +GI KF     LH  E ++G+  L   
Sbjct: 440 --AVDFPRINECYTGKKQRYVYGTILDSIAKV-TGIIKF----DLH-AEAETGKRMLEVG 499

Query: 537 SEEEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRF 596
                IK  Y + E   + + A +VPRE + +EDDG++I  VH+E T  S V ++DAK  
Sbjct: 500 GN---IKGIYDLGEGR-YGSEAIYVPRE-TAEEDDGYLIFFVHDENTGKSCVTVIDAKTM 527

Query: 597 SDEPVAKITLPHRVPYGFHGAFM 603
           S EPVA + LPHRVPYGFH  F+
Sbjct: 560 SAEPVAVVELPHRVPYGFHALFV 527

BLAST of CmaCh01G014360 vs. ExPASy Swiss-Prot
Match: Q94IR2 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris OX=3885 GN=CCD1 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.2e-61
Identity = 185/582 (31.79%), Postives = 286/582 (49.14%), Query Frame = 0

Query: 41  GKKLKQQQQHVRVDIVKKLKNG-CMKLVDGFVDWVFEF---SDQTLHPNQTNLAPVEEIG 100
           GKK   +   V+VD   K  NG   K++D     + +F   S    H    N  PV E  
Sbjct: 5   GKKNGAEGGLVKVD--PKPTNGFSSKVIDLLEKLLVKFLYDSSLPHHYLTGNFGPVTET- 64

Query: 101 APVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFK 160
            P     ++G LP     G F+R GPNP F  +         + + W +G+GM+H +  K
Sbjct: 65  PPTKDLPVKGHLPDCL-NGEFVRVGPNPKFAPV---------AGYHWFDGDGMIHGLRIK 124

Query: 161 KNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLN--WLRFGEV 220
              DGK   +Y +R+V+T   + E++   R  F+    GD   +    ++N   LR    
Sbjct: 125 ---DGK--ATYVSRFVETSRLKQEEYF-GRSKFMKI--GDLKGLFGLLMVNIHMLRTKLK 184

Query: 221 NKDMS------NTNVIEHSGKFYSVAENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTS 280
             D+S      NT ++ H GK  +++E   P  I +     LQ+LG+ D  +     FT+
Sbjct: 185 VLDLSYGGGTTNTALVYHHGKLLALSEADKPYAIKVFEDGDLQTLGMLDYDKRLGHSFTA 244

Query: 281 HPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNV 340
           HPK  P TGE+   G A T PY+   +IS+DG  M   V + +S   + H+  +T  + V
Sbjct: 245 HPKVDPFTGEMFSFGYAHTPPYITYRVISKDG-YMHDPVPITISDPIMMHDFAITENYAV 304

Query: 341 ILDYPVTIDLNRLIRGGSLI-KFEKEGYAKIGVMPRYG-DADSIQWFEVKPNC-TMHLFN 400
            +D P+      +++  +LI  F+    A+ GV+PRY  D   I+WFE+ PNC   H  N
Sbjct: 305 FMDLPLIFRPKEMVKNKTLIFSFDSTKKARFGVLPRYAKDEQHIRWFEL-PNCFIFHNAN 364

Query: 401 CFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEW 460
            +E ++EVV+  CR                   + +   V  T ++  E+ S  +  YE 
Sbjct: 365 AWEEEDEVVLITCRLQ-----------------NPKLDNVGGTVQEKLENFS--NELYEM 424

Query: 461 RLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLA 520
           R N+KTGEA ++ L+ +  ++DFP +N  +TG K +Y Y   LDS+A   +GI KF    
Sbjct: 425 RFNMKTGEASQKKLSAS--TVDFPRVNENYTGRKQRYVYGTTLDSIAKV-TGIIKF---- 484

Query: 521 KLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVPREDSL--QEDDGWIIAH 580
            LH  EP  G+  L      + +    + L    F + A ++PR   +  +EDDG+++  
Sbjct: 485 DLH-AEPDHGKEKLEVGGNVQGL----YDLGPGKFGSEAVYIPRVPGIESEEDDGYLVLF 532

Query: 581 VHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFM 603
           VH+E    S V+++DAK  S +PVA + LP+RVPYGFH  F+
Sbjct: 545 VHDENAGKSFVHVIDAKTMSADPVAVVELPNRVPYGFHAFFV 532

BLAST of CmaCh01G014360 vs. ExPASy TrEMBL
Match: A0A6J1IV34 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480255 PE=3 SV=1)

HSP 1 Score: 1267.3 bits (3278), Expect = 0.0e+00
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0

Query: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60
           MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK
Sbjct: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60

Query: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120
           NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN
Sbjct: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120

Query: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180
           GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE
Sbjct: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180

Query: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240
           KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ
Sbjct: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240

Query: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300
           QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM
Sbjct: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300

Query: 301 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 360
           VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR
Sbjct: 301 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 360

Query: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420
           YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK
Sbjct: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420

Query: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480
           PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG
Sbjct: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480

Query: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540
           YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG
Sbjct: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540

Query: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 600
           ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA
Sbjct: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 600

Query: 601 FMPISFNK 609
           FMPISFNK
Sbjct: 601 FMPISFNK 608

BLAST of CmaCh01G014360 vs. ExPASy TrEMBL
Match: A0A6J1J0T1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480255 PE=3 SV=1)

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 587/608 (96.55%), Postives = 587/608 (96.55%), Query Frame = 0

Query: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60
           MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK
Sbjct: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60

Query: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120
           NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN
Sbjct: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120

Query: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180
           GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE
Sbjct: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180

Query: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240
           KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ
Sbjct: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240

Query: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300
           QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM
Sbjct: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300

Query: 301 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 360
           VHKVDVKLSRSSLTHEIGVTRR                     LIKFEKEGYAKIGVMPR
Sbjct: 301 VHKVDVKLSRSSLTHEIGVTRR---------------------LIKFEKEGYAKIGVMPR 360

Query: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420
           YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK
Sbjct: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420

Query: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480
           PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG
Sbjct: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480

Query: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540
           YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG
Sbjct: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540

Query: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 600
           ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA
Sbjct: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 587

Query: 601 FMPISFNK 609
           FMPISFNK
Sbjct: 601 FMPISFNK 587

BLAST of CmaCh01G014360 vs. ExPASy TrEMBL
Match: A0A6J1FNM1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445898 PE=3 SV=1)

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 573/603 (95.02%), Postives = 590/603 (97.84%), Query Frame = 0

Query: 6   MMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLKNGCMK 65
           MM MFPGSS TRSPSISL+IHPCKSNLSSSMPLIPGKKLK  QQHVRVDIV+KLKNGCMK
Sbjct: 1   MMLMFPGSSPTRSPSISLSIHPCKSNLSSSMPLIPGKKLK--QQHVRVDIVQKLKNGCMK 60

Query: 66  LVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRNGPNPL 125
           LVD FVD VFEFSDQTLHPNQ+NLAPVEEIGAPVAV DIQGTLPQQFPQGIFLRNGPNPL
Sbjct: 61  LVDAFVDTVFEFSDQTLHPNQSNLAPVEEIGAPVAVADIQGTLPQQFPQGIFLRNGPNPL 120

Query: 126 FGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFEKHVKN 185
           FGGLK TRSM GRSSFIWVEGEGMLHA+YFKKN++G+WHL YNNRYVQT+SF+FEKHVKN
Sbjct: 121 FGGLKWTRSMLGRSSFIWVEGEGMLHAIYFKKNINGEWHLYYNNRYVQTDSFQFEKHVKN 180

Query: 186 RPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQQIDIM 245
           RPSFLPAVEGDA+A+VAAF+LNWLRFGEVNKDMSNTNVIEHSG+FYSVAENSMPQ+IDIM
Sbjct: 181 RPSFLPAVEGDALAVVAAFVLNWLRFGEVNKDMSNTNVIEHSGRFYSVAENSMPQEIDIM 240

Query: 246 SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVD 305
           SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVD
Sbjct: 241 SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVD 300

Query: 306 VKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDAD 365
           VKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDAD
Sbjct: 301 VKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDAD 360

Query: 366 SIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVT 425
           SIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVT
Sbjct: 361 SIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVT 420

Query: 426 DEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVL 485
           DEQN EDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKN+YGYAQVL
Sbjct: 421 DEQNAEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNRYGYAQVL 480

Query: 486 DSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVP 545
           DSVASSNSG+FKFGGLAKLHFEEPQSGEVSLLK SEEEPIKVEYHMLENNSFCTGASFVP
Sbjct: 481 DSVASSNSGMFKFGGLAKLHFEEPQSGEVSLLKGSEEEPIKVEYHMLENNSFCTGASFVP 540

Query: 546 REDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPIS 605
           REDSLQEDDGWIIAHVHNEIT TSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPIS
Sbjct: 541 REDSLQEDDGWIIAHVHNEITNTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPIS 600

Query: 606 FNK 609
           F+K
Sbjct: 601 FHK 601

BLAST of CmaCh01G014360 vs. ExPASy TrEMBL
Match: A0A6J1CDW2 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111010347 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 9.2e-270
Identity = 451/596 (75.67%), Postives = 512/596 (85.91%), Query Frame = 0

Query: 23  LAIHPCKSNLS-SSMPLIPGKKLKQQQQ--HVRVDIVKKLKNGCMKLVDGFVDWVFEFSD 82
           + IH C  + S    P+IP +K KQQ+Q   ++VDI+K LKN C+KL+D FVD VFEFSD
Sbjct: 3   MLIHACTPSSSIMPEPMIPMRKQKQQKQLLPLKVDILKNLKNSCIKLLDVFVDSVFEFSD 62

Query: 83  QTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRS 142
           QTLHP Q NLAPVEEIG  VAVT IQGT+PQ+FPQGI++RNGPNPLFGGLKST+S+FGRS
Sbjct: 63  QTLHPTQGNLAPVEEIGTAVAVTTIQGTIPQEFPQGIYIRNGPNPLFGGLKSTKSIFGRS 122

Query: 143 SFIWVEGEGMLHAMYFKKNMD--GKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDA 202
           ++ WVEGEGMLHA+YF K+     KW+L YNNRYVQT++F+ EKHVKN P FLPA+EGD+
Sbjct: 123 NYTWVEGEGMLHALYFNKDHTKLRKWNLFYNNRYVQTQTFQLEKHVKNGPYFLPAIEGDS 182

Query: 203 VAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQQIDIMSLQSLGVWDVCR 262
           +A+++AF LN LRFG VNKDMSNTNVIEHSGKFYSVA+NS+PQQIDIMSLQSLGVWDV  
Sbjct: 183 LAVLSAFFLNMLRFGTVNKDMSNTNVIEHSGKFYSVADNSLPQQIDIMSLQSLGVWDVNG 242

Query: 263 AWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEI 322
           AWNRPFTSHPKKAP TGELVIMG+ P KP+M +GIISEDGK MVHKVDVKLSR S +HEI
Sbjct: 243 AWNRPFTSHPKKAPGTGELVIMGVTPAKPFMLLGIISEDGKEMVHKVDVKLSRGSFSHEI 302

Query: 323 GVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCT 382
           GVTRR+NVILDYPVTID NRLI GGSLIK++KEGYA+IGVMPRYGD DSIQWF+VKPNCT
Sbjct: 303 GVTRRYNVILDYPVTIDFNRLITGGSLIKYDKEGYARIGVMPRYGDGDSIQWFQVKPNCT 362

Query: 383 MHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVTDEQNT------- 442
           MHLFN FE  +EVVVWGCRA+DS+IPGPEKG NKFEWFS RFKP+ + DEQN+       
Sbjct: 363 MHLFNSFEQNDEVVVWGCRASDSVIPGPEKGHNKFEWFSNRFKPLPIADEQNSDSCSSSM 422

Query: 443 EDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVAS 502
           EDGSL S AYEWRLNLKTGEARERFL+G QFSMDFPFINS FTGL+NK+GYAQ+LDS AS
Sbjct: 423 EDGSLFSRAYEWRLNLKTGEARERFLSGTQFSMDFPFINSRFTGLQNKFGYAQILDSFAS 482

Query: 503 SNSGIFKFGGLAKLHFEEP-QSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVPREDS 562
           SNSG+FKFGGLAKLHFEEP QS   SL K    E IKVEYHM EN+SFC+GASFVPRED 
Sbjct: 483 SNSGMFKFGGLAKLHFEEPDQSCGFSLPKKWGNEAIKVEYHMFENSSFCSGASFVPREDG 542

Query: 563 LQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPIS 606
            +EDDGWIIAH+HNEIT TSQVYI+DAK+FS+E VAKITLP RVPYGFHGAFMP++
Sbjct: 543 AEEDDGWIIAHLHNEITNTSQVYIIDAKKFSEEAVAKITLPQRVPYGFHGAFMPMN 598

BLAST of CmaCh01G014360 vs. ExPASy TrEMBL
Match: A0A1S3CQD3 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103503600 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 2.3e-244
Identity = 414/612 (67.65%), Postives = 492/612 (80.39%), Query Frame = 0

Query: 7   MWMFPGSSITRSPSI---SLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLKNGC 66
           M + P  S+ + PSI   S   H   SNLS  +P    KK  ++   ++VD+ KKLK   
Sbjct: 6   MIILPNCSLNQKPSIHPPSKLPHFSSSNLSIIIPTEKVKKTDREIIPLKVDVFKKLKTTW 65

Query: 67  MKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRNGPN 126
           MKL+DGFVD VFEFSDQTLHPNQ N APVEE+G+ V VT+IQGTLPQ FP G+++RNG N
Sbjct: 66  MKLLDGFVDSVFEFSDQTLHPNQINFAPVEEMGSAVTVTNIQGTLPQDFPHGVYIRNGGN 125

Query: 127 PLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGK-WHLSYNNRYVQTESFRFEKH 186
           PLFGGLK  +S+FG SSFIWVEGEGMLHA+YFKK  +GK W + YNNR+V+T+SFR EK 
Sbjct: 126 PLFGGLKWVKSIFGESSFIWVEGEGMLHALYFKKASNGKSWEVLYNNRFVETDSFRLEKL 185

Query: 187 VKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQQI 246
            K RP FLP VEGD+VA++ AFLLNW+RFG+ NKD+SNTNV EHSGKFYS+AEN +P QI
Sbjct: 186 EKKRPCFLPTVEGDSVAVLFAFLLNWVRFGKFNKDISNTNVFEHSGKFYSIAENHLPHQI 245

Query: 247 DIMSLQSLGVWDV-CRAW-NRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 306
           DI +L +LG WD+   AW NRPFTSHPKKAP+TGELVIMG+  TKP+ME+GIISEDGKRM
Sbjct: 246 DINTLHTLGNWDLDATAWNNRPFTSHPKKAPETGELVIMGVTSTKPFMEVGIISEDGKRM 305

Query: 307 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 366
           VHKVDVKLSRSSL+HEIGVT+R+NVILDY +T+D NRLIRGG  +K++KEGY++IGVMPR
Sbjct: 306 VHKVDVKLSRSSLSHEIGVTKRYNVILDYALTMDFNRLIRGGPFLKYDKEGYSRIGVMPR 365

Query: 367 YGDADSIQWFEVKPNCTMHLFNCFE-HQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRF 426
           YGD+DSI+WF VKPNC MHLFNCFE +Q+EVVVWGCRA DS+IPGPEKGLNKFEWFSQRF
Sbjct: 366 YGDSDSIKWFNVKPNCCMHLFNCFELNQHEVVVWGCRANDSVIPGPEKGLNKFEWFSQRF 425

Query: 427 ----KPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGL 486
                 + V +E++   GSLL  AY+WRLNL+TGE +E+ L      MDFPFIN  FTGL
Sbjct: 426 NNQNNKIVVNEEEDNNGGSLLPVAYQWRLNLRTGEVKEKCLIHQSIFMDFPFINLRFTGL 485

Query: 487 KNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENN 546
            NK+GYAQ+L S ASSNSG+FKFGGLAKL F++  S +   L D E   IKVE HMLE N
Sbjct: 486 PNKFGYAQLLHSSASSNSGMFKFGGLAKLQFQQHTSNDEFSLDDEE---IKVECHMLEEN 545

Query: 547 SFCTGASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPY 606
           S+C+GASFVPRE+  +EDDGWIIAHVHNEIT TSQVY++DA++FS+EP+AKITLP RVPY
Sbjct: 546 SYCSGASFVPRENGEEEDDGWIIAHVHNEITNTSQVYVLDARKFSEEPIAKITLPQRVPY 605

Query: 607 GFHGAFMPISFN 608
           GFHGAFMPI  N
Sbjct: 606 GFHGAFMPIKNN 614

BLAST of CmaCh01G014360 vs. NCBI nr
Match: XP_022980961.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1267.3 bits (3278), Expect = 0.0e+00
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0

Query: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60
           MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK
Sbjct: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60

Query: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120
           NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN
Sbjct: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120

Query: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180
           GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE
Sbjct: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180

Query: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240
           KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ
Sbjct: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240

Query: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300
           QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM
Sbjct: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300

Query: 301 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 360
           VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR
Sbjct: 301 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 360

Query: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420
           YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK
Sbjct: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420

Query: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480
           PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG
Sbjct: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480

Query: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540
           YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG
Sbjct: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540

Query: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 600
           ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA
Sbjct: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 600

Query: 601 FMPISFNK 609
           FMPISFNK
Sbjct: 601 FMPISFNK 608

BLAST of CmaCh01G014360 vs. NCBI nr
Match: XP_022980963.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 587/608 (96.55%), Postives = 587/608 (96.55%), Query Frame = 0

Query: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60
           MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK
Sbjct: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60

Query: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120
           NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN
Sbjct: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120

Query: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180
           GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE
Sbjct: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180

Query: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240
           KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ
Sbjct: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240

Query: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300
           QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM
Sbjct: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300

Query: 301 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 360
           VHKVDVKLSRSSLTHEIGVTRR                     LIKFEKEGYAKIGVMPR
Sbjct: 301 VHKVDVKLSRSSLTHEIGVTRR---------------------LIKFEKEGYAKIGVMPR 360

Query: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420
           YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK
Sbjct: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420

Query: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480
           PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG
Sbjct: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480

Query: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540
           YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG
Sbjct: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540

Query: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 600
           ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA
Sbjct: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGA 587

Query: 601 FMPISFNK 609
           FMPISFNK
Sbjct: 601 FMPISFNK 587

BLAST of CmaCh01G014360 vs. NCBI nr
Match: XP_022940198.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita moschata] >XP_022940199.1 carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 573/603 (95.02%), Postives = 590/603 (97.84%), Query Frame = 0

Query: 6   MMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLKNGCMK 65
           MM MFPGSS TRSPSISL+IHPCKSNLSSSMPLIPGKKLK  QQHVRVDIV+KLKNGCMK
Sbjct: 1   MMLMFPGSSPTRSPSISLSIHPCKSNLSSSMPLIPGKKLK--QQHVRVDIVQKLKNGCMK 60

Query: 66  LVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRNGPNPL 125
           LVD FVD VFEFSDQTLHPNQ+NLAPVEEIGAPVAV DIQGTLPQQFPQGIFLRNGPNPL
Sbjct: 61  LVDAFVDTVFEFSDQTLHPNQSNLAPVEEIGAPVAVADIQGTLPQQFPQGIFLRNGPNPL 120

Query: 126 FGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFEKHVKN 185
           FGGLK TRSM GRSSFIWVEGEGMLHA+YFKKN++G+WHL YNNRYVQT+SF+FEKHVKN
Sbjct: 121 FGGLKWTRSMLGRSSFIWVEGEGMLHAIYFKKNINGEWHLYYNNRYVQTDSFQFEKHVKN 180

Query: 186 RPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQQIDIM 245
           RPSFLPAVEGDA+A+VAAF+LNWLRFGEVNKDMSNTNVIEHSG+FYSVAENSMPQ+IDIM
Sbjct: 181 RPSFLPAVEGDALAVVAAFVLNWLRFGEVNKDMSNTNVIEHSGRFYSVAENSMPQEIDIM 240

Query: 246 SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVD 305
           SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVD
Sbjct: 241 SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRMVHKVD 300

Query: 306 VKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDAD 365
           VKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDAD
Sbjct: 301 VKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYGDAD 360

Query: 366 SIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVT 425
           SIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVT
Sbjct: 361 SIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFKPVHVT 420

Query: 426 DEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVL 485
           DEQN EDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKN+YGYAQVL
Sbjct: 421 DEQNAEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNRYGYAQVL 480

Query: 486 DSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVP 545
           DSVASSNSG+FKFGGLAKLHFEEPQSGEVSLLK SEEEPIKVEYHMLENNSFCTGASFVP
Sbjct: 481 DSVASSNSGMFKFGGLAKLHFEEPQSGEVSLLKGSEEEPIKVEYHMLENNSFCTGASFVP 540

Query: 546 REDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPIS 605
           REDSLQEDDGWIIAHVHNEIT TSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPIS
Sbjct: 541 REDSLQEDDGWIIAHVHNEITNTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFMPIS 600

Query: 606 FNK 609
           F+K
Sbjct: 601 FHK 601

BLAST of CmaCh01G014360 vs. NCBI nr
Match: XP_023523500.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 544/573 (94.94%), Postives = 561/573 (97.91%), Query Frame = 0

Query: 36  MPLIPGKKLKQQQQHVRVDIVKKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEI 95
           MPLIPGKKLKQQ   VRVDIVKKLKNGCMKLVD FVD VFEFSDQT+HPNQTNL+PVEEI
Sbjct: 1   MPLIPGKKLKQQD--VRVDIVKKLKNGCMKLVDAFVDTVFEFSDQTVHPNQTNLSPVEEI 60

Query: 96  GAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYF 155
           GAPVAV DIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHA+YF
Sbjct: 61  GAPVAVADIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAIYF 120

Query: 156 KKNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVN 215
           KK+++G+WHL YNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVA+VAAF+LNWLRFGEVN
Sbjct: 121 KKHINGEWHLLYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAVVAAFVLNWLRFGEVN 180

Query: 216 KDMSNTNVIEHSGKFYSVAENSMPQQIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGE 275
           KDMSNTNVIEHSG+FYSVAENSMPQ+IDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGE
Sbjct: 181 KDMSNTNVIEHSGRFYSVAENSMPQEIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGE 240

Query: 276 LVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDL 335
           LVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIG+TRRFNVILDYPVTIDL
Sbjct: 241 LVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGITRRFNVILDYPVTIDL 300

Query: 336 NRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGC 395
           NRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMH+FNCFEH++EVVVWGC
Sbjct: 301 NRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHVFNCFEHEHEVVVWGC 360

Query: 396 RATDSIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERF 455
           RATDS IPGPEKGLNKFEWFSQRFKPV VTDEQNTEDGSLLS AY+WRLNLKTGE RERF
Sbjct: 361 RATDSFIPGPEKGLNKFEWFSQRFKPVCVTDEQNTEDGSLLSRAYQWRLNLKTGEVRERF 420

Query: 456 LTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVS 515
           LTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVS
Sbjct: 421 LTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVS 480

Query: 516 LLKDSEEEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVD 575
           LLKDSEEEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGW+IAHVHNEIT TSQVYIVD
Sbjct: 481 LLKDSEEEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGWVIAHVHNEITNTSQVYIVD 540

Query: 576 AKRFSDEPVAKITLPHRVPYGFHGAFMPISFNK 609
           AKRFSDEPVAKITLPHRVPYGFHGAFMPISFNK
Sbjct: 541 AKRFSDEPVAKITLPHRVPYGFHGAFMPISFNK 571

BLAST of CmaCh01G014360 vs. NCBI nr
Match: KAG6608091.1 (Carotenoid 9,10(9',10')-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.1e-306
Identity = 515/571 (90.19%), Postives = 538/571 (94.22%), Query Frame = 0

Query: 1   MGRSGMMWMFPGSSITRSPSISLAIHPCKSNLSSSMPLIPGKKLKQQQQHVRVDIVKKLK 60
           MGRSG+M MFPGSSITRSPSISL+IHPCKSNLSSSMPLIPGKKLK  QQHVRVDIVKKLK
Sbjct: 29  MGRSGVMLMFPGSSITRSPSISLSIHPCKSNLSSSMPLIPGKKLK--QQHVRVDIVKKLK 88

Query: 61  NGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRN 120
           NGCMKLVDGFVD VFEFSDQTLHPNQTNLAPVEEIGAPVAV DIQGTLPQQFPQGIFLRN
Sbjct: 89  NGCMKLVDGFVDSVFEFSDQTLHPNQTNLAPVEEIGAPVAVADIQGTLPQQFPQGIFLRN 148

Query: 121 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 180
           GPNPLFGGLKS+RS+ GRSSFIWVEGEGMLHA+YFKKN++G+WHL YNNRYVQT+SF+FE
Sbjct: 149 GPNPLFGGLKSSRSILGRSSFIWVEGEGMLHAIYFKKNINGEWHLYYNNRYVQTDSFQFE 208

Query: 181 KHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEVNKDMSNTNVIEHSGKFYSVAENSMPQ 240
           KHVKNRPSFLPAVEGDA+A+VAAF+LNWLRFGEVNKDMSNTNVIEHSG+FYSVAENSMPQ
Sbjct: 209 KHVKNRPSFLPAVEGDALAVVAAFVLNWLRFGEVNKDMSNTNVIEHSGRFYSVAENSMPQ 268

Query: 241 QIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIISEDGKRM 300
           +IDIMSLQSLGVWDVCRAWNRPFTSHPK       L+ M I P           EDGKRM
Sbjct: 269 EIDIMSLQSLGVWDVCRAWNRPFTSHPKVRFQI--LLFMSIGPDG--------DEDGKRM 328

Query: 301 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 360
           VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR
Sbjct: 329 VHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPR 388

Query: 361 YGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWGCRATDSIIPGPEKGLNKFEWFSQRFK 420
           YGDADSIQWFEVKPNCTMHLFNCFEH++EV+VWGCRATDS IPGPEKGLNKFEWFSQRFK
Sbjct: 389 YGDADSIQWFEVKPNCTMHLFNCFEHEHEVLVWGCRATDSFIPGPEKGLNKFEWFSQRFK 448

Query: 421 PVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFINSAFTGLKNKYG 480
           PV VTDEQNTEDGSLLS AY+WR+NLKTGE RERFLTGNQFSMDFPFINSAFTGLKNKYG
Sbjct: 449 PVRVTDEQNTEDGSLLSRAYQWRVNLKTGEVRERFLTGNQFSMDFPFINSAFTGLKNKYG 508

Query: 481 YAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 540
           YAQVLDSVASSNSG+FKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG
Sbjct: 509 YAQVLDSVASSNSGMFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTG 568

Query: 541 ASFVPREDSLQEDDGWIIAHVHNEITKTSQV 572
           ASFVPREDSLQEDDGW+IAHVHNEIT TSQV
Sbjct: 569 ASFVPREDSLQEDDGWVIAHVHNEITNTSQV 587

BLAST of CmaCh01G014360 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 244.2 bits (622), Expect = 2.7e-64
Identity = 177/563 (31.44%), Postives = 273/563 (48.49%), Query Frame = 0

Query: 57  KKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGI 116
           K   +  + L++  V  +   +   LH    N AP+ +   PV    + G LP+    G 
Sbjct: 20  KGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVHGFLPECL-NGE 79

Query: 117 FLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTES 176
           F+R GPNP F  +         + + W +G+GM+H +  K   DGK   +Y +RYV+T  
Sbjct: 80  FVRVGPNPKFDAV---------AGYHWFDGDGMIHGVRIK---DGK--ATYVSRYVKTSR 139

Query: 177 FRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRF--------GEVNKDMSNTNVIEHSG 236
            + E+       F+    GD        ++N  +                +NT ++ H G
Sbjct: 140 LKQEEFF-GAAKFMKI--GDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHG 199

Query: 237 KFYSVAENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTK 296
           K  ++ E   P  I ++    LQ+LG+ D  +     FT+HPK  P TGE+   G + T 
Sbjct: 200 KLLALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTP 259

Query: 297 PYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLI 356
           PY+   +IS+DG  M   V + +S   + H+  +T  + + +D P+      +++   +I
Sbjct: 260 PYLTYRVISKDG-IMHDPVPITISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMI 319

Query: 357 -KFEKEGYAKIGVMPRYG-DADSIQWFEVKPNC-TMHLFNCFEHQNEVVVWGCRATD--- 416
             F+    A+ GV+PRY  D   I+WFE+ PNC   H  N +E ++EVV+  CR  +   
Sbjct: 320 YSFDPTKKARFGVLPRYAKDELMIRWFEL-PNCFIFHNANAWEEEDEVVLITCRLENPDL 379

Query: 417 SIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGN 476
            ++ G  K   K E F                        YE R N+KTG A ++ L+ +
Sbjct: 380 DMVSGKVK--EKLENFGNEL--------------------YEMRFNMKTGSASQKKLSAS 439

Query: 477 QFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKD 536
             ++DFP IN  +TG K +Y Y  +LDS+A   +GI KF     LH  E ++G+  L   
Sbjct: 440 --AVDFPRINECYTGKKQRYVYGTILDSIAKV-TGIIKF----DLH-AEAETGKRMLEVG 499

Query: 537 SEEEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRF 596
                IK  Y + E   + + A +VPRE + +EDDG++I  VH+E T  S V ++DAK  
Sbjct: 500 GN---IKGIYDLGEGR-YGSEAIYVPRE-TAEEDDGYLIFFVHDENTGKSCVTVIDAKTM 527

Query: 597 SDEPVAKITLPHRVPYGFHGAFM 603
           S EPVA + LPHRVPYGFH  F+
Sbjct: 560 SAEPVAVVELPHRVPYGFHALFV 527

BLAST of CmaCh01G014360 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 196.1 bits (497), Expect = 8.5e-50
Identity = 171/621 (27.54%), Postives = 286/621 (46.05%), Query Frame = 0

Query: 13  SSITRSPSISLAIHPCKS----NLSSSMPLIPGKKLKQQQQHVRVDIVKKLKNGCMKLVD 72
           S +TR  ++S A+H   +      SS+ P I  K   ++    ++++ ++     +   +
Sbjct: 42  SRVTRKLNVSSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAE 101

Query: 73  GFVDWVFEFSDQTLHPNQTNLAPVEEIG---APVAVTDIQGTLP--QQFP---QGIFLRN 132
           GF+      S + LHP      P  +I    APV    ++  LP   + P   +G+++RN
Sbjct: 102 GFL-----VSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRN 161

Query: 133 GPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFE 192
           G NPL         + G   F   +G+GM+HA+ F+         SY  R+ QT  F  E
Sbjct: 162 GANPLH------EPVTGHHFF---DGDGMVHAVKFEHG-----SASYACRFTQTNRFVQE 221

Query: 193 KHVKNRPSFLPAV-EGDAVAIVAAFLLNWLRFGEVNKD------MSNTNVIEHSGKFYSV 252
           + +  RP F  A+ E      +A  +L + R      D      ++N  ++  +G+  ++
Sbjct: 222 RQL-GRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAM 281

Query: 253 AENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGI-APTKPYME 312
           +E+ +P Q+ I     L+++G +D          +HPK  P++GEL  +     +KPY++
Sbjct: 282 SEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLK 341

Query: 313 IGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEK 372
               S DG +    V+++L + ++ H+  +T  F V+ D  V   L  +IRGGS + ++K
Sbjct: 342 YFRFSPDGTKS-PDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDK 401

Query: 373 EGYAKIGVMPRYG-DADSIQWFEVKPNCTMHLFNCFE--HQNEVVVWGCRAT--DSIIPG 432
              A+ G++ +Y  D+ +I+W +       HL+N +E    +EVVV G   T  DSI   
Sbjct: 402 NKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIF-- 461

Query: 433 PEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGN---QF 492
                                   N  D +L S   E RLNLKTGE+  R +  N   Q 
Sbjct: 462 ------------------------NESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQV 521

Query: 493 SMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSE 552
           +++   +N    G K K+ Y  + +          K  G AK+   +  +GE        
Sbjct: 522 NLEAGMVNRNMLGRKTKFAYLALAEPWP-------KVSGFAKV---DLTTGE-------- 581

Query: 553 EEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSD 603
                V+ H+  +N +     F+P E   +ED+G+I+  VH+E T  S++ IV+A   S 
Sbjct: 582 -----VKKHLYGDNRYGGEPLFLPGEGG-EEDEGYILCFVHDEKTWKSELQIVNA--VSL 589

BLAST of CmaCh01G014360 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 177.9 bits (450), Expect = 2.4e-44
Identity = 147/557 (26.39%), Postives = 244/557 (43.81%), Query Frame = 0

Query: 66  LVDGFVDWVFEFSDQTLHPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRNGPNPL 125
           +++ F+D     S    H    N APV +   P     I GTLP     G ++RNGPNP 
Sbjct: 92  VINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSL-NGAYIRNGPNPQ 151

Query: 126 FGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFEKH--V 185
           F           R  +   +G+GMLHA+   K  +GK  L   +RYV+T  +  EK    
Sbjct: 152 F---------LPRGPYHLFDGDGMLHAI---KIHNGKATLC--SRYVKTYKYNVEKQTGA 211

Query: 186 KNRPSFLPAVEGDAV-----AIVAAFLLNWLRFGEVNK-DMSNTNVIEHSGKFYSVAENS 245
              P+      G        A+ AA +L   ++  VN   ++NT++   S + +++ E+ 
Sbjct: 212 PVMPNVFSGFNGVTASVARGALTAARVLTG-QYNPVNGIGLANTSLAFFSNRLFALGESD 271

Query: 246 MPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKKAPDTGELVIMGIAPTKPYMEIGIIS 305
           +P  + +     ++++G +D         T+HPK  P TGE       P  P++      
Sbjct: 272 LPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYFRFD 331

Query: 306 EDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTIDLNRL---IRGGSLIKFEKEG 365
             GK+        ++  S  H+  +T+R  +  +  + + +N L   + GGS +  +   
Sbjct: 332 SAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGK 391

Query: 366 YAKIGVMPRY-GDADSIQWFEVKPNCTMHLFNCFEHQ--NEVVVWGCRATDSIIPGPEKG 425
             ++GV+P+Y GD   ++WFEV     +H  N ++    N VV+        I P     
Sbjct: 392 TPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVL--------IAP----N 451

Query: 426 LNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNLKTGEARERFLTGNQFSMDFPFI 485
           +   E   +R   VH   E             + +++L TG  R   ++    ++DF  I
Sbjct: 452 IMSIEHTLERMDLVHALVE-------------KVKIDLVTGIVRRHPISAR--NLDFAVI 511

Query: 486 NSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVE 545
           N AF G  ++Y YA + D +        K  G+ KL            +   + +   V 
Sbjct: 512 NPAFLGRCSRYVYAAIGDPMP-------KISGVVKLD-----------VSKGDRDDCTVA 571

Query: 546 YHMLENNSFCTGASFVPRE---DSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVA 603
             M  +  +     FV R+      +EDDG+++ +VH+E+T  S+  ++DAK    E VA
Sbjct: 572 RRMYGSGCYGGEPFFVARDPGNPEAEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVA 587

BLAST of CmaCh01G014360 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 172.2 bits (435), Expect = 1.3e-42
Identity = 143/516 (27.71%), Postives = 236/516 (45.74%), Query Frame = 0

Query: 104 IQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMYFKKNMDGKW 163
           + GT+P+   QG+++RNG NPL       + + G   F   +G+GM+HA+ F        
Sbjct: 201 VTGTVPECI-QGVYVRNGANPLH------KPVSGHHLF---DGDGMVHAVRFDNG----- 260

Query: 164 HLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDA---VAIVAAFLLNWL-RFGEVNK--- 223
            +SY  R+ +T     E+    RP F P   G+    + I    L N    FG V+    
Sbjct: 261 SVSYACRFTETNRLVQEREC-GRPVF-PKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGG 320

Query: 224 -DMSNTNVIEHSGKFYSVAENSMPQQIDIM---SLQSLGVWDVCRAWNRPFTSHPKKAPD 283
             ++N  ++  +G   +++E+ +P  + +     L++ G +D          +HPK  P+
Sbjct: 321 LGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPE 380

Query: 284 TGELVIMGI-APTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPV 343
           T EL  +     +KPY++    + DG++    V++ L + ++ H+  +T  F VI D  V
Sbjct: 381 TRELFALSYDVVSKPYLKYFRFTSDGEKS-PDVEIPLDQPTMIHDFAITENFVVIPDQQV 440

Query: 344 TIDLNRLIRGGSLIKFEKEGYAKIGVMPRYG-DADSIQWFEVKPNCTMHLFNCFE--HQN 403
              L  +IRGGS + ++++  ++ G++ +   DA SIQW EV      HL+N +E    +
Sbjct: 441 VFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETD 500

Query: 404 EVVVWGCRAT--DSIIPGPEKGLNKFEWFSQRFKPVHVTDEQNTEDGSLLSCAYEWRLNL 463
           EVVV G   T  DSI                           N  D +L S   E RLNL
Sbjct: 501 EVVVIGSCMTPPDSIF--------------------------NEHDETLQSVLSEIRLNL 560

Query: 464 KTGEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHF 523
           KTGE+  R +   Q +++   +N    G K +Y Y  + +          K  G AK+  
Sbjct: 561 KTGESTRRPVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWP-------KVSGFAKV-- 620

Query: 524 EEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEIT 583
            +  +GE             +  ++     +     F+P  D  +ED G+I+  VH+E  
Sbjct: 621 -DLSTGE-------------IRKYIYGEGKYGGEPLFLPSGDG-EEDGGYIMVFVHDEEK 646

Query: 584 KTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFM 603
             S++ +++A     E  A +TLP RVPYGFHG F+
Sbjct: 681 VKSELQLINAVNMKLE--ATVTLPSRVPYGFHGTFI 646

BLAST of CmaCh01G014360 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 169.9 bits (429), Expect = 6.5e-42
Identity = 154/539 (28.57%), Postives = 249/539 (46.20%), Query Frame = 0

Query: 83  HPNQTNLAPVEEIGAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFI 142
           H    N APV E     +++ + G +P     G++LRNG NPLF        + G   F 
Sbjct: 111 HQISGNYAPVPEQSVKSSLS-VDGKIPDCI-DGVYLRNGANPLF------EPVSGHHLF- 170

Query: 143 WVEGEGMLHAMYFKKNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAV-EGDAVAIV 202
             +G+GM+HA+   K  +G    SY+ R+ +TE    EK +   P F  A+ E    + +
Sbjct: 171 --DGDGMVHAV---KITNG--DASYSCRFTETERLVQEKQL-GSPIFPKAIGELHGHSGI 230

Query: 203 AAFLLNWLR--FGEVN----KDMSNTNVIEHSGKFYSVAENSMPQQI---DIMSLQSLGV 262
           A  +L + R  FG +N      ++N  ++    +  +++E+ +P Q+   D   L+++G 
Sbjct: 231 ARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGR 290

Query: 263 WDVCRAWNRPFTSHPKKAPDTGELVIMGI-APTKPYMEIGIISEDGKRMVHKVDVKLSRS 322
           +D     +    +HPK  P T EL  +      KPY++    S +G++    V++ L+  
Sbjct: 291 FDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKS-PDVEIPLASP 350

Query: 323 SLTHEIGVTRRFNVILDYPVTIDLNRLIRGGSLIKFEKEGYAKIGVMPRYG-DADSIQWF 382
           ++ H+  +T  F VI D  V   L+ +  G S +K++ E  ++ G++PR   DA  + W 
Sbjct: 351 TMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWV 410

Query: 383 EVKPNCTMHLFNCFE--HQNEVVVWGCRAT--DSIIPGPEKGLNKFEWFSQRFKPVHVTD 442
           E       HL+N +E    +EVVV G   T  DSI                         
Sbjct: 411 ESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIF------------------------ 470

Query: 443 EQNTEDGSLLSCAYEWRLNLKTGEA-RERFLTGN-QFSMDFPFINSAFTGLKNKYGYAQV 502
             N  D  L S   E RLNLKTG++ R   + G+ Q +++   +N    G K +Y Y  +
Sbjct: 471 --NECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAI 530

Query: 503 LDSVASSNSGIFKFGGLAKLHFEEPQSGEVSLLKDSEEEPIKVEYHMLENNSFCTGASFV 562
            +          K  G AK+   +  +GE             V+ H      +     F+
Sbjct: 531 AEPWP-------KVSGFAKV---DLSTGE-------------VKNHFYGGKKYGGEPFFL 580

Query: 563 PRE-DSLQEDDGWIIAHVHNEITKTSQVYIVDAKRFSDEPVAKITLPHRVPYGFHGAFM 603
           PR  +S  EDDG+I++ VH+E +  S+++IV+A     E  A + LP RVPYGFHG F+
Sbjct: 591 PRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELE--ATVKLPSRVPYGFHGTFV 580

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LP177.4e-6731.96Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum OX=3888 GN=CCD1 ... [more]
Q84KG51.3e-6632.21Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus OX=82528 GN=CCD P... [more]
C3VEQ41.0e-6331.38Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Oncidium hybrid cultivar OX=14... [more]
O655723.8e-6331.44Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana OX=3702 G... [more]
Q94IR21.2e-6131.79Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris OX=3885 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1IV340.0e+00100.00carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X1 OS=Cucurbita maxi... [more]
A0A6J1J0T10.0e+0096.55carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X2 OS=Cucurbita maxi... [more]
A0A6J1FNM10.0e+0095.02carotenoid 9,10(9',10')-cleavage dioxygenase 1-like OS=Cucurbita moschata OX=366... [more]
A0A6J1CDW29.2e-27075.67carotenoid 9,10(9',10')-cleavage dioxygenase 1-like OS=Momordica charantia OX=36... [more]
A0A1S3CQD32.3e-24467.65carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X3 OS=Cucumis melo O... [more]
Match NameE-valueIdentityDescription
XP_022980961.10.0e+00100.00carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X1 [Cucurbita maxima... [more]
XP_022980963.10.0e+0096.55carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X2 [Cucurbita maxima... [more]
XP_022940198.10.0e+0095.02carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita moschata] >XP_022... [more]
XP_023523500.10.0e+0094.94carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6608091.11.1e-30690.19Carotenoid 9,10(9',10')-cleavage dioxygenase, partial [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
AT3G63520.12.7e-6431.44carotenoid cleavage dioxygenase 1 [more]
AT3G14440.18.5e-5027.54nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT4G19170.12.4e-4426.39nine-cis-epoxycarotenoid dioxygenase 4 [more]
AT1G78390.11.3e-4227.71nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT1G30100.16.5e-4228.57nine-cis-epoxycarotenoid dioxygenase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 93..603
e-value: 1.1E-94
score: 318.2
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 37..604
NoneNo IPR availablePANTHERPTHR10543:SF30OS06G0162550 PROTEINcoord: 37..604

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G014360.1CmaCh01G014360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen