CmaCh01G014300 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G014300
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	D-amino-acid transaminase, chloroplastic
Location	Cma_Chr01: 10061966 .. 10064296 (+)
RNA-Seq Expression	CmaCh01G014300
Synteny	CmaCh01G014300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAGGTCGGTCAGGTATTCAGCAATTGAACAAAACATTTTCTCAAATTATACTGAACATTAATCTCCGATGAATGCTCTGCAGTCTCTTCTCAAACCCATTCCTCAGTCACCGATCAGCGGTGCAGAACTCACTGCCCATTCTCAAAGGCCGCTAGGCAAATCCTGCAATTCTCGGCAGTGGTCTTTTAGAAGATTCAGGATTAGGTGTTCGATTGCTGGTTAGTTTTTTTTCAAATTCGTCGCGAGAATGTTTGATAATTGGTTCCACTGGAGCCTTGTTTATCGAAGCAAGTTCGTTTGAAACTGTTTAATAGTTGACTCGCTTTTCATAATCTGGTTTTTTTACGACACTGTTCGGGCTTTTGCGATCCTGATTCACTTTTCATGATCTGGTTGTTTACGATACTGTTCAGGCTTTTGCGATCCTAACTGTTATTCAATTTTCTCTATCCGTTCAATCTGAAATCTGAATAATCAAAGCAACTGCTCGTCTTGTCGCAGATACCGTTGATCGTTCTTCTGATGCTCCAGTGCTTGGTAGTTCACAGGTCAACTCTTGTTGTGTATTGTAATCTGTTTGTGTGTTGAAATGAACTACAAATTTTAGTGTTCTATGCGTGGAAGGTGATAATTGTATAGCTTTACTGATTGTTATTTCGATCCATGTGTTTATTGAAGGTGCTTGAAAGATTAAGGGCAAGCCGAGAGAATCAAGAAAACCAGCCACAATTCCTTGCAATGTATTCCAGTGTTGTTGGGGGAATTACGACAGATCCAGCTGTTATGGTAATTCCAATTGATGACCACATGGTTCATCGGGGACATGGCGTTTTTGATACTGCTGCCATAGTGGATGGGTATGTGAGAATTTCATAACGTTTCTTCAAGATTATTGTGAAATCCCACATTGGTTGGGAGGAGAACGAAACATCCTTTATAAGGGTGTGGACTTGGGCCGTTACAATTATGAATCTGATATCGATTTCCCAAGACCCGCATAATTAGCACTGTAAATTGATAGGTTCTTATGAACATTTGAATTCATAACTAAATTGATATATATTTTTCTTCATTGGAAGTAGGAAAGGCATACTTTCATTTCATGATTTTGTCAGTGTTGAGTTACTGTATAAAATGGTTTTGATCTCAGGCATCTCTATGAGTTGGATCAGCACCTCGACCGCATTTTAAGATCAGCATCCATGGCAAAAATCAACCTCCCAGTTTCATATGATCGAGAGAAGATTAAAAATATACTCATAAGAACTGCCAGTGCTTCTAAGTGTAGGAATGGACAGCTTCGATATTGGCTCTCTGCAGGACCTGGAAATTTTCTACTATCTACTTCTGGATTGCATCAGCCAGCCCTTTATGCAATTGTAATTCAAGGTAAGCCGCCATCTCCTCCAAAAGGCATCAAAGTCATAACTTCATCAATCCCAATGAAACCACCTCAATTTGCAACTATGAAGAGTGTAAATTACCTTCCAAATGTTCTTTCAAATATGGAAGCAGAAGAAAAGGGTGCTTATACAGCCATTTGGTTGGATAGTGAGGGATTCATTGCTGAAGGGCCTAGTATGAACGTTGGTTTTATTACAAAGGATAAGGAGTTTCTGATGCCTCACTTTGACAAAATTTTAAGCGGCTGCACAGCTAAGAGAATCATAACTCTCGCTGAGCGGCTGGTGAAAGAGGGTAGGCTTCGGAGTATAAGATTTGAAAATTTAACCATGGAGGAAGGCAAGAAGGCAGAGGAAATGGTGCTTATTGGGAGTGGAGTTCTTGTTTCCCCTGTACTGCAGTGGGATGAGCAGATTATTGGTGATGGTAAGTATACCCTCTATAATCCTTAATTCATAGATACGATGTTTTTGTAGTTCTGAATCATGTTGTGATTGGCTGTATGAATTTCTTCTAACATGTTTAAAATTCCATTTAAAGTACCGGGGAGGGAGAGTGTTTTTGTAAGCACTTAGAAAGTCGTTCTAAACCGGTTTTAAGATTAGTTTTGTAGAATGACGACACCATGACATGTTTTGATGTGTCGAGCATCGTTCTTATGAATAGGAACACACATAGTTTTCTTCCCACCGCAACTTTATGGTGATCTAAGAAAGCCATCGTTATAATATTTCTGATGATGGGGATGAGTGTTCTAATTCTATAACAAGAACACGAAAGCATGTTTTTATCTGACAAATTAAGAATGTTTTGGTTTTCCTCCACAGGAAGAGAAGGTCCATTGGCTCGAGCACTTGTAGATCTTATTATCGAAGACATGAAATCCGGCCCTCTAACCGTTCGAATACCAGTTCTATATGAATAA

mRNA sequence

AGGAGGTCGGTCAGGTATTCAGCAATTGAACAAAACATTTTCTCAAATTATACTGAACATTAATCTCCGATGAATGCTCTGCAGTCTCTTCTCAAACCCATTCCTCAGTCACCGATCAGCGGTGCAGAACTCACTGCCCATTCTCAAAGGCCGCTAGGCAAATCCTGCAATTCTCGGCAGTGGTCTTTTAGAAGATTCAGGATTAGGTGTTCGATTGCTGATACCGTTGATCGTTCTTCTGATGCTCCAGTGCTTGGTAGTTCACAGGTGCTTGAAAGATTAAGGGCAAGCCGAGAGAATCAAGAAAACCAGCCACAATTCCTTGCAATGTATTCCAGTGTTGTTGGGGGAATTACGACAGATCCAGCTGTTATGGTAATTCCAATTGATGACCACATGGTTCATCGGGGACATGGCGTTTTTGATACTGCTGCCATAGTGGATGGGCATCTCTATGAGTTGGATCAGCACCTCGACCGCATTTTAAGATCAGCATCCATGGCAAAAATCAACCTCCCAGTTTCATATGATCGAGAGAAGATTAAAAATATACTCATAAGAACTGCCAGTGCTTCTAAGTGTAGGAATGGACAGCTTCGATATTGGCTCTCTGCAGGACCTGGAAATTTTCTACTATCTACTTCTGGATTGCATCAGCCAGCCCTTTATGCAATTGTAATTCAAGGTAAGCCGCCATCTCCTCCAAAAGGCATCAAAGTCATAACTTCATCAATCCCAATGAAACCACCTCAATTTGCAACTATGAAGAGTGTAAATTACCTTCCAAATGTTCTTTCAAATATGGAAGCAGAAGAAAAGGGTGCTTATACAGCCATTTGGTTGGATAGTGAGGGATTCATTGCTGAAGGGCCTAGTATGAACGTTGGTTTTATTACAAAGGATAAGGAGTTTCTGATGCCTCACTTTGACAAAATTTTAAGCGGCTGCACAGCTAAGAGAATCATAACTCTCGCTGAGCGGCTGGTGAAAGAGGGTAGGCTTCGGAGTATAAGATTTGAAAATTTAACCATGGAGGAAGGCAAGAAGGCAGAGGAAATGGTGCTTATTGGGAGTGGAGTTCTTGTTTCCCCTGTACTGCAGTGGGATGAGCAGATTATTGGTGATGGAAGAGAAGGTCCATTGGCTCGAGCACTTGTAGATCTTATTATCGAAGACATGAAATCCGGCCCTCTAACCGTTCGAATACCAGTTCTATATGAATAA

Coding sequence (CDS)

ATGAATGCTCTGCAGTCTCTTCTCAAACCCATTCCTCAGTCACCGATCAGCGGTGCAGAACTCACTGCCCATTCTCAAAGGCCGCTAGGCAAATCCTGCAATTCTCGGCAGTGGTCTTTTAGAAGATTCAGGATTAGGTGTTCGATTGCTGATACCGTTGATCGTTCTTCTGATGCTCCAGTGCTTGGTAGTTCACAGGTGCTTGAAAGATTAAGGGCAAGCCGAGAGAATCAAGAAAACCAGCCACAATTCCTTGCAATGTATTCCAGTGTTGTTGGGGGAATTACGACAGATCCAGCTGTTATGGTAATTCCAATTGATGACCACATGGTTCATCGGGGACATGGCGTTTTTGATACTGCTGCCATAGTGGATGGGCATCTCTATGAGTTGGATCAGCACCTCGACCGCATTTTAAGATCAGCATCCATGGCAAAAATCAACCTCCCAGTTTCATATGATCGAGAGAAGATTAAAAATATACTCATAAGAACTGCCAGTGCTTCTAAGTGTAGGAATGGACAGCTTCGATATTGGCTCTCTGCAGGACCTGGAAATTTTCTACTATCTACTTCTGGATTGCATCAGCCAGCCCTTTATGCAATTGTAATTCAAGGTAAGCCGCCATCTCCTCCAAAAGGCATCAAAGTCATAACTTCATCAATCCCAATGAAACCACCTCAATTTGCAACTATGAAGAGTGTAAATTACCTTCCAAATGTTCTTTCAAATATGGAAGCAGAAGAAAAGGGTGCTTATACAGCCATTTGGTTGGATAGTGAGGGATTCATTGCTGAAGGGCCTAGTATGAACGTTGGTTTTATTACAAAGGATAAGGAGTTTCTGATGCCTCACTTTGACAAAATTTTAAGCGGCTGCACAGCTAAGAGAATCATAACTCTCGCTGAGCGGCTGGTGAAAGAGGGTAGGCTTCGGAGTATAAGATTTGAAAATTTAACCATGGAGGAAGGCAAGAAGGCAGAGGAAATGGTGCTTATTGGGAGTGGAGTTCTTGTTTCCCCTGTACTGCAGTGGGATGAGCAGATTATTGGTGATGGAAGAGAAGGTCCATTGGCTCGAGCACTTGTAGATCTTATTATCGAAGACATGAAATCCGGCCCTCTAACCGTTCGAATACCAGTTCTATATGAATAA

Protein sequence

MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLARALVDLIIEDMKSGPLTVRIPVLYE

Homology

[Close]

BLAST of CmaCh01G014300 vs. ExPASy Swiss-Prot
Match: Q8L493 (D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.6e-120
Identity = 221/349 (63.32%), Postives = 279/349 (79.94%), Query Frame = 0


Query: 36  RQWSFRRFRIRCSIADTVDRSSDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGI 95
           R+ + RR  + CS  D+  +S + PVL S +V ERL+ +R  Q    QFLAMYSSVV GI
Sbjct: 33  RRLTRRRTILMCS--DSSSQSWNVPVLSSYEVGERLKLARGGQ----QFLAMYSSVVDGI 92

Query: 96  TTDPAVMVIPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDR 155
           TTDPA MV+P+DDHMVHRGHGVFDTA I++G+LYELDQHLDRILRSASMAKI LP  +DR
Sbjct: 93  TTDPAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLP--FDR 152

Query: 156 EKIKNILIRTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGI 215
           E IK ILI+T S S CR+G LRYWLSAGPG+FLLS S   +P LYAIVI+      P G+
Sbjct: 153 ETIKRILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINPIGV 212

Query: 216 KVITSSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFI 275
           KV+TSSIP+KPP+FAT+KSVNYLPNVLS MEAE KGAY  IW+  +GFIAEGP+MNV F+
Sbjct: 213 KVVTSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFV 272

Query: 276 TK-DKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIG 335
               KE +MP FD +LSGCTAKR +TLAE+LV +G L++++  ++T+E+GKKA+EM+LIG
Sbjct: 273 VNGGKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIG 332

Query: 336 SGVLVSPVLQWDEQIIGDGREGPLARALVDLIIEDMKSGPLTVRIPVLY 384
           SG+ + PV+QWDE+ IG+G+EGP+A+AL+DL++EDM+SGP +VR+ V Y
Sbjct: 333 SGIPIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of CmaCh01G014300 vs. ExPASy Swiss-Prot
Match: Q58414 (Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=ilvE PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.6e-22
Identity = 72/261 (27.59%), Postives = 131/261 (50.19%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILI 163
           + + DH +  G GVF+     DG ++ L +H+DR+  SA    I++P++  +E++ ++++
Sbjct: 17  VSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLT--KEEMIDVVL 76

Query: 164 RTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIP 223
            T   +  R+  +R  ++ G G+  L      +P ++ I I   P     GI+ IT S+ 
Sbjct: 77  ETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGIRAITVSVR 136

Query: 224 MKPPQF--ATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEF 283
             P       +KS+NYL +VL+ ++A   G   A  LD +GF+ EG   N+  +      
Sbjct: 137 RLPVDVLNPAVKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLK 196

Query: 284 LMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSP 343
             P +  IL G T   +I LA    KE  +  +  E LT+ +   A+E+ + G+   + P
Sbjct: 197 TPPVYQSILKGITRDVVIKLA----KEEGIEVVE-EPLTLHDLYTADELFITGTAAEIVP 256

Query: 344 VLQWDEQIIGDGREGPLARAL 363
           V + D ++I + + G + + L
Sbjct: 257 VFEIDGRVINNKQVGEITKKL 270

[Close]

BLAST of CmaCh01G014300 vs. ExPASy Swiss-Prot
Match: O29329 (Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=ilvE PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.8e-22
Identity = 77/274 (28.10%), Postives = 136/274 (49.64%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILI 163
           + I DH    G GVF+     +G ++ L +H+DR+  SA    + +P++  +E+   I++
Sbjct: 18  VSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHIDRLYDSAKAIDLEIPIT--KEEFMEIIL 77

Query: 164 RTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSP------PKGIKV 223
            T   +  R+  +R  ++ G G+  L       P   +I++  KP          KG+  
Sbjct: 78  ETLRKNNLRDAYIRPIVTRGIGDLGLDPRKCQNP---SIIVITKPWGKLYGDLYEKGLTA 137

Query: 224 IT-----SSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNV 283
           IT     +S    PP    +KS+NYL N+L+ +EA  KG   AI+LD  G+++EG   N+
Sbjct: 138 ITVAVRRNSFDALPP---NIKSLNYLNNILAKIEANAKGGDEAIFLDRNGYVSEGSGDNI 197

Query: 284 GFITKDKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFE--NLTMEEGKKAEEM 343
            F+ K+     P     L G T + +I +  RL        I F+  N+ + +   A+E+
Sbjct: 198 -FVVKNGAITTPPTINNLRGITREAVIEIINRL-------GIPFKETNIGLYDLYTADEV 257

Query: 344 VLIGSGVLVSPVLQWDEQIIGDGREGPLARALVD 365
            + G+   ++P++  D + IGDG+ G + R L++
Sbjct: 258 FVTGTAAEIAPIVVIDGRKIGDGKPGEITRKLME 275

[Close]

BLAST of CmaCh01G014300 vs. ExPASy Swiss-Prot
Match: Q9ASR4 (Branched-chain-amino-acid aminotransferase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g27410 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.4e-18
Identity = 82/271 (30.26%), Postives = 137/271 (50.55%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE+IK  +
Sbjct: 275 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT---REEIKEAI 334

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPAL------YAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 335 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAFNRYGCTLIVLAEWKPPVYDNDG 394

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+       AI LD +GF++E  + N
Sbjct: 395 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATN 454

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMV 343
           +  + KD+  L PH D  L G T     T+ E +VKE  +   R   +++ E   A+E+ 
Sbjct: 455 IFMVKKDR-VLTPHADYCLPGITR---ATVMELVVKENFILEER--RISLSEFHTADEVW 514

Query: 344 LIGSGVLVSPVLQWDEQIIGDGREGPLARAL 363
             G+   +SPV++ D ++IG+G+ GP+ R L
Sbjct: 515 TTGTMGELSPVVKIDGRVIGEGKVGPVTRRL 531

[Close]

BLAST of CmaCh01G014300 vs. ExPASy Swiss-Prot
Match: Q8W0Z7 (Branched-chain-amino-acid aminotransferase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At3g05190 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.1e-18
Identity = 82/271 (30.26%), Postives = 136/271 (50.18%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE++K  +
Sbjct: 278 VSVFDSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKALAFDNVPA---REEVKEAI 337

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPA------LYAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 338 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAYNRYGCTLIVLAEWKPPVYDNEG 397

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+    A  AI LD +G+++E  + N
Sbjct: 398 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETNATN 457

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMV 343
           + F+ K    L PH D  L G T     T+ E +VKE  +   R   +++ E   A E+ 
Sbjct: 458 I-FMVKKGCVLTPHADYCLPGITR---ATVMELVVKENFILEER--RISLSEFHTANEVW 517

Query: 344 LIGSGVLVSPVLQWDEQIIGDGREGPLARAL 363
             G+   +SPV++ D ++IGDG+ GP+ R L
Sbjct: 518 TTGTMGELSPVVKIDGRVIGDGKVGPVTRTL 534

[Close]

BLAST of CmaCh01G014300 vs. ExPASy TrEMBL
Match: A0A6J1IST6 (D-amino-acid transaminase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111480287 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 4.8e-216
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60
           MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP
Sbjct: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 ALVDLIIEDMKSGPLTVRIPVLYE 385
           ALVDLIIEDMKSGPLTVRIPVLYE
Sbjct: 361 ALVDLIIEDMKSGPLTVRIPVLYE 384

[Close]

BLAST of CmaCh01G014300 vs. ExPASy TrEMBL
Match: A0A6J1FI15 (D-amino-acid transaminase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445949 PE=4 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.2e-208
Identity = 372/384 (96.88%), Postives = 376/384 (97.92%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60
           MN+LQ LLKPIPQSPISGAELTAHSQRPL KSCNSRQ SFRRFRIRCSIADTVDR SDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLVKEGRLR IRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRGIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 ALVDLIIEDMKSGPLTVRIPVLYE 385
           A+VDLIIEDMK GP TVRIP+ Y+
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYK 384

[Close]

BLAST of CmaCh01G014300 vs. ExPASy TrEMBL
Match: A0A6J1CNI4 (D-amino-acid transaminase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111012818 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.1e-171
Identity = 316/393 (80.41%), Postives = 341/393 (86.77%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPL----------GKSCNSRQWSFRRFRIRCSIA 60
           M +LQSLLKP+    IS  E     QRPL           +S NSRQ S R FRIR SIA
Sbjct: 1   MASLQSLLKPVSHPLISPTEFFYDFQRPLRFARLLSLSVHESRNSRQLSSRGFRIRSSIA 60

Query: 61  DTVDRSSDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHM 120
           DT+  SSDAPVL SS+VLERLRASREN E+Q QFLAMYSSV GGITTDPA MVIPIDDHM
Sbjct: 61  DTIGHSSDAPVLSSSEVLERLRASRENLESQQQFLAMYSSVFGGITTDPAAMVIPIDDHM 120

Query: 121 VHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK 180
           VHRGHGVFDTAAIVDG+LYELDQHLDRILRSASMAKINLPV YDRE IK ILIRT SASK
Sbjct: 121 VHRGHGVFDTAAIVDGYLYELDQHLDRILRSASMAKINLPVPYDRETIKRILIRTVSASK 180

Query: 181 CRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFA 240
           CRNG LRYWLSAGPG+F LS+SG ++P+LYA+VIQGKPPS PKGIKVITSS+PMKPPQFA
Sbjct: 181 CRNGSLRYWLSAGPGDFQLSSSGCYEPSLYAVVIQGKPPSHPKGIKVITSSVPMKPPQFA 240

Query: 241 TMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKIL 300
            MKSVNYLPNVLS MEAEEKGAY +IWLD++GFIAEGP+MNV FIT DKEFLMPHFDKIL
Sbjct: 241 IMKSVNYLPNVLSKMEAEEKGAYASIWLDNDGFIAEGPNMNVAFITSDKEFLMPHFDKIL 300

Query: 301 SGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQII 360
           SGCTAKRI+TLAE+LVKEGRLRSIR ENLTMEEGKKA+EM+LIGSGVLV PVLQWDEQII
Sbjct: 301 SGCTAKRILTLAEQLVKEGRLRSIRCENLTMEEGKKADEMMLIGSGVLVCPVLQWDEQII 360

Query: 361 GDGREGPLARALVDLIIEDMKSGPLTVRIPVLY 384
           GDGREGPLA+AL+DL+IEDMKSGP TVRIPV Y
Sbjct: 361 GDGREGPLAQALLDLLIEDMKSGPSTVRIPVPY 393

[Close]

BLAST of CmaCh01G014300 vs. ExPASy TrEMBL
Match: A0A5D3E5X5 (D-amino-acid transaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004930 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 5.4e-167
Identity = 305/393 (77.61%), Postives = 338/393 (86.01%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKS----------CNSRQWSFRRFRIRCSIA 60
           M +LQS+L+PIPQ+  S A L   SQ  +  +           N+R+  FR FRI  SIA
Sbjct: 1   MASLQSILRPIPQATNSPAVLPHRSQSSIRFTRFRSHHVQILRNTRRLYFRGFRIMASIA 60

Query: 61  DTVDRSSDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHM 120
           DTVD +SDAPVL SS+V+ERLRA RENQENQ ++LAMYSSV GGITTDPA MVIPIDDHM
Sbjct: 61  DTVDHTSDAPVLTSSEVIERLRARRENQENQQEYLAMYSSVFGGITTDPAAMVIPIDDHM 120

Query: 121 VHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK 180
           VHRGHGVFDTAAIVDG+LYELDQHLDRIL+SASMAKINLP+ YDRE I+ ILIRT SASK
Sbjct: 121 VHRGHGVFDTAAIVDGYLYELDQHLDRILKSASMAKINLPIPYDREMIRRILIRTVSASK 180

Query: 181 CRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFA 240
           CRNG LRYWLSAGPG+F LS+SG H  ALYA+VIQGKPPS PKGIKVITSS+PMKPPQFA
Sbjct: 181 CRNGSLRYWLSAGPGDFQLSSSGCHLSALYAVVIQGKPPSRPKGIKVITSSVPMKPPQFA 240

Query: 241 TMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKIL 300
            MKSVNYLPNVLS MEAEEKGAY +IWLDS+GFIAEGP+MNV FIT DKEF+MPHFDKIL
Sbjct: 241 IMKSVNYLPNVLSKMEAEEKGAYASIWLDSDGFIAEGPNMNVAFITSDKEFIMPHFDKIL 300

Query: 301 SGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQII 360
           SGCTAKRII LAERLVKEG+LRSI  EN+TMEEGKKA+EM+LIGSGVLVSPVLQWDEQII
Sbjct: 301 SGCTAKRIINLAERLVKEGKLRSISCENITMEEGKKADEMMLIGSGVLVSPVLQWDEQII 360

Query: 361 GDGREGPLARALVDLIIEDMKSGPLTVRIPVLY 384
           GDG+EGPL +AL+DL+IEDMKSGP TVRIPV Y
Sbjct: 361 GDGKEGPLVQALLDLLIEDMKSGPPTVRIPVPY 393

[Close]

BLAST of CmaCh01G014300 vs. ExPASy TrEMBL
Match: A0A1S3CQM1 (D-amino-acid transaminase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503592 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 5.4e-167
Identity = 305/393 (77.61%), Postives = 338/393 (86.01%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKS----------CNSRQWSFRRFRIRCSIA 60
           M +LQS+L+PIPQ+  S A L   SQ  +  +           N+R+  FR FRI  SIA
Sbjct: 1   MASLQSILRPIPQATNSPAVLPHRSQSSIRFTRFRSHHVQILRNTRRLYFRGFRIMASIA 60

Query: 61  DTVDRSSDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHM 120
           DTVD +SDAPVL SS+V+ERLRA RENQENQ ++LAMYSSV GGITTDPA MVIPIDDHM
Sbjct: 61  DTVDHTSDAPVLTSSEVIERLRARRENQENQQEYLAMYSSVFGGITTDPAAMVIPIDDHM 120

Query: 121 VHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK 180
           VHRGHGVFDTAAIVDG+LYELDQHLDRIL+SASMAKINLP+ YDRE I+ ILIRT SASK
Sbjct: 121 VHRGHGVFDTAAIVDGYLYELDQHLDRILKSASMAKINLPIPYDREMIRRILIRTVSASK 180

Query: 181 CRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFA 240
           CRNG LRYWLSAGPG+F LS+SG H  ALYA+VIQGKPPS PKGIKVITSS+PMKPPQFA
Sbjct: 181 CRNGSLRYWLSAGPGDFQLSSSGCHLSALYAVVIQGKPPSRPKGIKVITSSVPMKPPQFA 240

Query: 241 TMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKIL 300
            MKSVNYLPNVLS MEAEEKGAY +IWLDS+GFIAEGP+MNV FIT DKEF+MPHFDKIL
Sbjct: 241 IMKSVNYLPNVLSKMEAEEKGAYASIWLDSDGFIAEGPNMNVAFITSDKEFIMPHFDKIL 300

Query: 301 SGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQII 360
           SGCTAKRII LAERLVKEG+LRSI  EN+TMEEGKKA+EM+LIGSGVLVSPVLQWDEQII
Sbjct: 301 SGCTAKRIINLAERLVKEGKLRSISCENITMEEGKKADEMMLIGSGVLVSPVLQWDEQII 360

Query: 361 GDGREGPLARALVDLIIEDMKSGPLTVRIPVLY 384
           GDG+EGPL +AL+DL+IEDMKSGP TVRIPV Y
Sbjct: 361 GDGKEGPLVQALLDLLIEDMKSGPPTVRIPVPY 393

[Close]

BLAST of CmaCh01G014300 vs. NCBI nr
Match: XP_022981012.1 (D-amino-acid transaminase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 760.0 bits (1961), Expect = 9.9e-216
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60
           MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP
Sbjct: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 ALVDLIIEDMKSGPLTVRIPVLYE 385
           ALVDLIIEDMKSGPLTVRIPVLYE
Sbjct: 361 ALVDLIIEDMKSGPLTVRIPVLYE 384

[Close]

BLAST of CmaCh01G014300 vs. NCBI nr
Match: KAG7031718.1 (D-amino-acid transaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 735.3 bits (1897), Expect = 2.6e-208
Identity = 373/384 (97.14%), Postives = 376/384 (97.92%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60
           MN+LQ LLKPIPQSPISGAELTAHSQRPL KSCNSRQ SFRRFRIRCSIADTVDR SDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLV EGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 ALVDLIIEDMKSGPLTVRIPVLYE 385
           A+VDLIIEDMK GP TVRIP+ YE
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYE 384

[Close]

BLAST of CmaCh01G014300 vs. NCBI nr
Match: XP_022940281.1 (D-amino-acid transaminase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 734.6 bits (1895), Expect = 4.5e-208
Identity = 372/384 (96.88%), Postives = 376/384 (97.92%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60
           MN+LQ LLKPIPQSPISGAELTAHSQRPL KSCNSRQ SFRRFRIRCSIADTVDR SDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLVKEGRLR IRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRGIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 ALVDLIIEDMKSGPLTVRIPVLYE 385
           A+VDLIIEDMK GP TVRIP+ Y+
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYK 384

[Close]

BLAST of CmaCh01G014300 vs. NCBI nr
Match: XP_023521525.1 (D-amino-acid transaminase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 729.2 bits (1881), Expect = 1.9e-206
Identity = 372/384 (96.88%), Postives = 374/384 (97.40%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60
           MN+LQSLLKPIPQSPIS AELTAHSQRPL  SCNSRQ SFRRFRIRCSIADTVDR+ DAP
Sbjct: 1   MNSLQSLLKPIPQSPISDAELTAHSQRPLNNSCNSRQLSFRRFRIRCSIADTVDRTFDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQ ALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQSALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLV EGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 ALVDLIIEDMKSGPLTVRIPVLYE 385
           ALVDLIIEDMKSGP TVRI V YE
Sbjct: 361 ALVDLIIEDMKSGPPTVRIRVPYE 384

[Close]

BLAST of CmaCh01G014300 vs. NCBI nr
Match: KAG6608083.1 (D-amino-acid transaminase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 657.1 bits (1694), Expect = 9.1e-185
Identity = 343/384 (89.32%), Postives = 347/384 (90.36%), Query Frame = 0


Query: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60
           MN+LQ LLKPIPQSPISGAELTAHSQRPL KSCNSRQ SFRRFRIRCSIADTVDR SDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK          
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK---------- 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
                            + YAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 ----------------SSFYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLV EGRLR IRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVTEGRLRGIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 358

Query: 361 ALVDLIIEDMKSGPLTVRIPVLYE 385
           A+VDLIIEDMK GP TVRIP+ YE
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYE 358

[Close]

BLAST of CmaCh01G014300 vs. TAIR 10
Match: AT5G57850.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-121
Identity = 221/349 (63.32%), Postives = 279/349 (79.94%), Query Frame = 0


Query: 36  RQWSFRRFRIRCSIADTVDRSSDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGI 95
           R+ + RR  + CS  D+  +S + PVL S +V ERL+ +R  Q    QFLAMYSSVV GI
Sbjct: 33  RRLTRRRTILMCS--DSSSQSWNVPVLSSYEVGERLKLARGGQ----QFLAMYSSVVDGI 92

Query: 96  TTDPAVMVIPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDR 155
           TTDPA MV+P+DDHMVHRGHGVFDTA I++G+LYELDQHLDRILRSASMAKI LP  +DR
Sbjct: 93  TTDPAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLP--FDR 152

Query: 156 EKIKNILIRTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGI 215
           E IK ILI+T S S CR+G LRYWLSAGPG+FLLS S   +P LYAIVI+      P G+
Sbjct: 153 ETIKRILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINPIGV 212

Query: 216 KVITSSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFI 275
           KV+TSSIP+KPP+FAT+KSVNYLPNVLS MEAE KGAY  IW+  +GFIAEGP+MNV F+
Sbjct: 213 KVVTSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFV 272

Query: 276 TK-DKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIG 335
               KE +MP FD +LSGCTAKR +TLAE+LV +G L++++  ++T+E+GKKA+EM+LIG
Sbjct: 273 VNGGKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIG 332

Query: 336 SGVLVSPVLQWDEQIIGDGREGPLARALVDLIIEDMKSGPLTVRIPVLY 384
           SG+ + PV+QWDE+ IG+G+EGP+A+AL+DL++EDM+SGP +VR+ V Y
Sbjct: 333 SGIPIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of CmaCh01G014300 vs. TAIR 10
Match: AT5G27410.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 1.7e-19
Identity = 82/271 (30.26%), Postives = 137/271 (50.55%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE+IK  +
Sbjct: 275 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT---REEIKEAI 334

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPAL------YAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 335 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAFNRYGCTLIVLAEWKPPVYDNDG 394

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+       AI LD +GF++E  + N
Sbjct: 395 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATN 454

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMV 343
           +  + KD+  L PH D  L G T     T+ E +VKE  +   R   +++ E   A+E+ 
Sbjct: 455 IFMVKKDR-VLTPHADYCLPGITR---ATVMELVVKENFILEER--RISLSEFHTADEVW 514

Query: 344 LIGSGVLVSPVLQWDEQIIGDGREGPLARAL 363
             G+   +SPV++ D ++IG+G+ GP+ R L
Sbjct: 515 TTGTMGELSPVVKIDGRVIGEGKVGPVTRRL 531

[Close]

BLAST of CmaCh01G014300 vs. TAIR 10
Match: AT5G27410.2 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 1.7e-19
Identity = 82/271 (30.26%), Postives = 137/271 (50.55%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE+IK  +
Sbjct: 652 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT---REEIKEAI 711

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPAL------YAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 712 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAFNRYGCTLIVLAEWKPPVYDNDG 771

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+       AI LD +GF++E  + N
Sbjct: 772 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATN 831

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMV 343
           +  + KD+  L PH D  L G T     T+ E +VKE  +   R   +++ E   A+E+ 
Sbjct: 832 IFMVKKDR-VLTPHADYCLPGITR---ATVMELVVKENFILEER--RISLSEFHTADEVW 891

Query: 344 LIGSGVLVSPVLQWDEQIIGDGREGPLARAL 363
             G+   +SPV++ D ++IG+G+ GP+ R L
Sbjct: 892 TTGTMGELSPVVKIDGRVIGEGKVGPVTRRL 908

[Close]

BLAST of CmaCh01G014300 vs. TAIR 10
Match: AT3G05190.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 2.2e-19
Identity = 82/271 (30.26%), Postives = 136/271 (50.18%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE++K  +
Sbjct: 278 VSVFDSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKALAFDNVPA---REEVKEAI 337

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPA------LYAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 338 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAYNRYGCTLIVLAEWKPPVYDNEG 397

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+    A  AI LD +G+++E  + N
Sbjct: 398 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETNATN 457

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLAERLVKEGRLRSIRFENLTMEEGKKAEEMV 343
           + F+ K    L PH D  L G T     T+ E +VKE  +   R   +++ E   A E+ 
Sbjct: 458 I-FMVKKGCVLTPHADYCLPGITR---ATVMELVVKENFILEER--RISLSEFHTANEVW 517

Query: 344 LIGSGVLVSPVLQWDEQIIGDGREGPLARAL 363
             G+   +SPV++ D ++IGDG+ GP+ R L
Sbjct: 518 TTGTMGELSPVVKIDGRVIGDGKVGPVTRTL 534

The following BLAST results are available for this feature:

BLAST of CmaCh01G014300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L493	1.6e-120	63.32	D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT...	[more]
Q58414	1.6e-22	27.59	Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannas...	[more]
O29329	7.8e-22	28.10	Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (s...	[more]
Q9ASR4	2.4e-18	30.26	Branched-chain-amino-acid aminotransferase-like protein 2 OS=Arabidopsis thalian...	[more]
Q8W0Z7	3.1e-18	30.26	Branched-chain-amino-acid aminotransferase-like protein 1 OS=Arabidopsis thalian...	[more]

BLAST of CmaCh01G014300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IST6	4.8e-216	100.00	D-amino-acid transaminase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A6J1FI15	2.2e-208	96.88	D-amino-acid transaminase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1CNI4	2.1e-171	80.41	D-amino-acid transaminase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5D3E5X5	5.4e-167	77.61	D-amino-acid transaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CQM1	5.4e-167	77.61	D-amino-acid transaminase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503592...	[more]

BLAST of CmaCh01G014300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022981012.1	9.9e-216	100.00	D-amino-acid transaminase, chloroplastic [Cucurbita maxima]	[more]
KAG7031718.1	2.6e-208	97.14	D-amino-acid transaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022940281.1	4.5e-208	96.88	D-amino-acid transaminase, chloroplastic [Cucurbita moschata]	[more]
XP_023521525.1	1.9e-206	96.88	D-amino-acid transaminase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
KAG6608083.1	9.1e-185	89.32	D-amino-acid transaminase, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of CmaCh01G014300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G57850.1	1.2e-121	63.32	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT5G27410.1	1.7e-19	30.26	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT5G27410.2	1.7e-19	30.26	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT3G05190.1	2.2e-19	30.26	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043131	Branched-chain-amino-acid aminotransferase-like, N-terminal	GENE3D	3.30.470.10		coord: 56..204 e-value: 1.4E-23 score: 85.4
IPR043132	Branched-chain-amino-acid aminotransferase-like, C-terminal	GENE3D	3.20.10.10		coord: 210..374 e-value: 9.7E-38 score: 131.2
IPR001544	Aminotransferase class IV	PFAM	PF01063	Aminotran_4	coord: 116..344 e-value: 2.6E-42 score: 145.2
None	No IPR available	PANTHER	PTHR42743:SF8	BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE-LIKE	coord: 56..382
None	No IPR available	PANTHER	PTHR42743	AMINO-ACID AMINOTRANSFERASE	coord: 56..382
IPR036038	Aminotransferase-like, PLP-dependent enzymes	SUPERFAMILY	56752	D-aminoacid aminotransferase-like PLP-dependent enzymes	coord: 93..370

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G014300.1	CmaCh01G014300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019752	carboxylic acid metabolic process
molecular_function	GO:0008483	transaminase activity
molecular_function	GO:0003824	catalytic activity