CmaCh01G014220 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G014220
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDNA-directed RNA polymerase subunit beta'
LocationCma_Chr01: 10020043 .. 10020654 (-)
RNA-Seq ExpressionCmaCh01G014220
SyntenyCmaCh01G014220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGAACCCTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGCCAGAAAAGAATCAATTTAGATAGTCCTTTGTGGCTCCGTTGGCGACTAGATCAACGCATTATTGCTTCAAGAGAAGCTCCTATCGAAGTTCACTATGAATTTTTGGGTACCCATCATGAGATTTACAGGTACTATCTAATAGTCAAAAGTATAAAAAAAGAAATTCTTTGTATATACATTCGAACCACTGTTGGGTTATATTATGAACTCTATTCAAAGTGTTTGAATCCAAAATCTAAAATTTTAAATAAGTTCAAAAGATTATTCGTCTAAGCGAGATGTTCGGTTATCTTTTTTTTAACATTATTGCACTAAATCACCATTACATTGTTGGAAGGTTGAGGGAAACGGCAAAACATAAGATTTTGATATTTTGAGGTAAGAAGAGTGAGGGAGTGCCGACAGCCGACAGCCGACGTAGACGACACCGACAGCTTTTTTGGGAGACACAAAATCCACACTTTGTTACAATGTTTGTTTTTGGTGTATCATCATCATCTCTTCTCTTCTTCCCAAACAATTCAAATCAAAATCCAACCCAAAATTAATGGATCCCTTTAA

mRNA sequence

ATGAAAGAACCCTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGCCAGAAAAGAATCAATTTAGATAGTCCTTTGTGGCTCCGTTGGCGACTAGATCAACGCATTATTGCTTCAAGAGAAGCTCCTATCGAAGTTCACTATGAATTTTTGGGTACCCATCATGAGATTTACAGAGTGAGGGAGTGCCGACAGCCGACAGCCGACGTAGACGACACCGACAGCTTTTTTGGGAGACACAAAATCCACACTTTGTTACAATGTTTGTTTTTGGTGTATCATCATCATCTCTTCTCTTCTTCCCAAACAATTCAAATCAAAATCCAACCCAAAATTAATGGATCCCTTTAA

Coding sequence (CDS)

ATGAAAGAACCCTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGCCAGAAAAGAATCAATTTAGATAGTCCTTTGTGGCTCCGTTGGCGACTAGATCAACGCATTATTGCTTCAAGAGAAGCTCCTATCGAAGTTCACTATGAATTTTTGGGTACCCATCATGAGATTTACAGAGTGAGGGAGTGCCGACAGCCGACAGCCGACGTAGACGACACCGACAGCTTTTTTGGGAGACACAAAATCCACACTTTGTTACAATGTTTGTTTTTGGTGTATCATCATCATCTCTTCTCTTCTTCCCAAACAATTCAAATCAAAATCCAACCCAAAATTAATGGATCCCTTTAA

Protein sequence

MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIYRVRECRQPTADVDDTDSFFGRHKIHTLLQCLFLVYHHHLFSSSQTIQIKIQPKINGSL
Homology
BLAST of CmaCh01G014220 vs. ExPASy Swiss-Prot
Match: Q4VZP2 (DNA-directed RNA polymerase subunit beta' OS=Cucumis sativus OX=3659 GN=rpoC1 PE=3 SV=3)

HSP 1 Score: 127.1 bits (318), Expect = 1.3e-28
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 577 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 634

BLAST of CmaCh01G014220 vs. ExPASy Swiss-Prot
Match: B1A926 (DNA-directed RNA polymerase subunit beta' OS=Carica papaya OX=3649 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-27
Identity = 54/58 (93.10%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRW+LDQR+IASREAPIEVHYE LGT+HEIY
Sbjct: 577 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWQLDQRVIASREAPIEVHYESLGTYHEIY 634

BLAST of CmaCh01G014220 vs. ExPASy Swiss-Prot
Match: Q49L07 (DNA-directed RNA polymerase subunit beta' OS=Eucalyptus globulus subsp. globulus OX=71271 GN=rpoC1 PE=3 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-27
Identity = 54/58 (93.10%), Postives = 56/58 (96.55%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASRE PIE+HYE LGT+HEIY
Sbjct: 579 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREVPIEIHYESLGTYHEIY 636

BLAST of CmaCh01G014220 vs. ExPASy Swiss-Prot
Match: B2XWP0 (DNA-directed RNA polymerase subunit beta' OS=Fagopyrum esculentum subsp. ancestrale OX=180217 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-27
Identity = 53/59 (89.83%), Postives = 57/59 (96.61%), Query Frame = 0

Query: 1   MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           MKEPFFCNSYDAIGAYRQKRINLDSPLWLRW+LD R++ASREAPIEVHYE LGT+HEIY
Sbjct: 572 MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWQLDHRVVASREAPIEVHYESLGTYHEIY 630

BLAST of CmaCh01G014220 vs. ExPASy Swiss-Prot
Match: Q589B9 (DNA-directed RNA polymerase subunit beta' OS=Silene latifolia OX=37657 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 3.2e-27
Identity = 54/59 (91.53%), Postives = 57/59 (96.61%), Query Frame = 0

Query: 1   MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           M+EPFFCNSYDAIGAY QKRINLDSPLWLRWRLDQRIIASREAP+EVHYE LGT+HEIY
Sbjct: 579 MEEPFFCNSYDAIGAYCQKRINLDSPLWLRWRLDQRIIASREAPVEVHYESLGTYHEIY 637

BLAST of CmaCh01G014220 vs. ExPASy TrEMBL
Match: A0A6H0ESX8 (DNA-directed RNA polymerase subunit beta' OS=Cyclantheropsis parviflora OX=387129 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 9.7e-27
Identity = 57/59 (96.61%), Postives = 58/59 (98.31%), Query Frame = 0

Query: 1   MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 576 MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 634

BLAST of CmaCh01G014220 vs. ExPASy TrEMBL
Match: A0A218KG20 (DNA-directed RNA polymerase subunit beta' OS=Cucumis sativus var. hardwickii OX=319220 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.8e-26
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 577 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 634

BLAST of CmaCh01G014220 vs. ExPASy TrEMBL
Match: A0A6M3S6B8 (DNA-directed RNA polymerase subunit beta' OS=Trichosanthes kirilowii OX=3677 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.8e-26
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 578 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 635

BLAST of CmaCh01G014220 vs. ExPASy TrEMBL
Match: A0A1X9Q1T5 (DNA-directed RNA polymerase subunit beta' OS=Cucumis sativus OX=3659 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.8e-26
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 577 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 634

BLAST of CmaCh01G014220 vs. ExPASy TrEMBL
Match: A0A249RZJ2 (DNA-directed RNA polymerase subunit beta' OS=Cucumis melo var. cantalupensis OX=3658 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.8e-26
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 578 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 635

BLAST of CmaCh01G014220 vs. NCBI nr
Match: YP_009752741.1 (RNA polymerase beta' subunit [Cyclantheropsis parviflora] >QIT05391.1 RNA polymerase beta' subunit [Cyclantheropsis parviflora])

HSP 1 Score: 129.4 bits (324), Expect = 2.0e-26
Identity = 57/59 (96.61%), Postives = 58/59 (98.31%), Query Frame = 0

Query: 1   MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 576 MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 634

BLAST of CmaCh01G014220 vs. NCBI nr
Match: QSX42912.1 (RNA polymerase beta' subunit [Peganum harmala])

HSP 1 Score: 127.1 bits (318), Expect = 1.0e-25
Identity = 56/59 (94.92%), Postives = 58/59 (98.31%), Query Frame = 0

Query: 1   MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGT+HEIY
Sbjct: 580 MKEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTYHEIY 638

BLAST of CmaCh01G014220 vs. NCBI nr
Match: YP_247590.2 (RNA polymerase beta' subunit [Cucumis sativus])

HSP 1 Score: 127.1 bits (318), Expect = 1.0e-25
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 577 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 634

BLAST of CmaCh01G014220 vs. NCBI nr
Match: ASY96794.1 (RNA polymerase beta' subunit [Cucumis melo var. momordica] >ASY96968.1 RNA polymerase beta' subunit [Cucumis melo var. cantalupo] >ASY97316.1 RNA polymerase beta' subunit [Cucumis melo var. flexuosus] >ASY97055.1 RNA polymerase beta' subunit [Cucumis melo var. cantalupo] >ASY97229.1 RNA polymerase beta' subunit [Cucumis melo var. cantalupo])

HSP 1 Score: 127.1 bits (318), Expect = 1.0e-25
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 578 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 635

BLAST of CmaCh01G014220 vs. NCBI nr
Match: YP_009751644.1 (RNA polymerase beta' subunit [Hodgsonia heteroclita] >QIT04377.1 RNA polymerase beta' subunit [Hodgsonia heteroclita])

HSP 1 Score: 127.1 bits (318), Expect = 1.0e-25
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQR+IASREAPIEVHYE LGTHHEIY
Sbjct: 577 KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRVIASREAPIEVHYESLGTHHEIY 634

BLAST of CmaCh01G014220 vs. TAIR 10
Match: ATCG00180.1 (DNA-directed RNA polymerase family protein )

HSP 1 Score: 117.1 bits (292), Expect = 9.6e-27
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 2   KEPFFCNSYDAIGAYRQKRINLDSPLWLRWRLDQRIIASREAPIEVHYEFLGTHHEIY 60
           KEPFFCNSYDAIGAYRQK+INLDSPLWLRW+LDQR+IASRE PIEVHYE  G +HEIY
Sbjct: 577 KEPFFCNSYDAIGAYRQKKINLDSPLWLRWQLDQRVIASREVPIEVHYESFGNYHEIY 634

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4VZP21.3e-2896.55DNA-directed RNA polymerase subunit beta' OS=Cucumis sativus OX=3659 GN=rpoC1 PE... [more]
B1A9261.9e-2793.10DNA-directed RNA polymerase subunit beta' OS=Carica papaya OX=3649 GN=rpoC1 PE=3... [more]
Q49L071.9e-2793.10DNA-directed RNA polymerase subunit beta' OS=Eucalyptus globulus subsp. globulus... [more]
B2XWP01.9e-2789.83DNA-directed RNA polymerase subunit beta' OS=Fagopyrum esculentum subsp. ancestr... [more]
Q589B93.2e-2791.53DNA-directed RNA polymerase subunit beta' OS=Silene latifolia OX=37657 GN=rpoC1 ... [more]
Match NameE-valueIdentityDescription
A0A6H0ESX89.7e-2796.61DNA-directed RNA polymerase subunit beta' OS=Cyclantheropsis parviflora OX=38712... [more]
A0A218KG204.8e-2696.55DNA-directed RNA polymerase subunit beta' OS=Cucumis sativus var. hardwickii OX=... [more]
A0A6M3S6B84.8e-2696.55DNA-directed RNA polymerase subunit beta' OS=Trichosanthes kirilowii OX=3677 GN=... [more]
A0A1X9Q1T54.8e-2696.55DNA-directed RNA polymerase subunit beta' OS=Cucumis sativus OX=3659 GN=rpoC1 PE... [more]
A0A249RZJ24.8e-2696.55DNA-directed RNA polymerase subunit beta' OS=Cucumis melo var. cantalupensis OX=... [more]
Match NameE-valueIdentityDescription
YP_009752741.12.0e-2696.61RNA polymerase beta' subunit [Cyclantheropsis parviflora] >QIT05391.1 RNA polyme... [more]
QSX42912.11.0e-2594.92RNA polymerase beta' subunit [Peganum harmala][more]
YP_247590.21.0e-2596.55RNA polymerase beta' subunit [Cucumis sativus][more]
ASY96794.11.0e-2596.55RNA polymerase beta' subunit [Cucumis melo var. momordica] >ASY96968.1 RNA polym... [more]
YP_009751644.11.0e-2596.55RNA polymerase beta' subunit [Hodgsonia heteroclita] >QIT04377.1 RNA polymerase ... [more]
Match NameE-valueIdentityDescription
ATCG00180.19.6e-2786.21DNA-directed RNA polymerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042102RNA polymerase Rpb1, domain 3 superfamilyGENE3D1.10.274.100RNA polymerase Rpb1, domain 3coord: 1..112
e-value: 6.1E-6
score: 28.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G014220.1CmaCh01G014220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0009507 chloroplast
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0008270 zinc ion binding