CmaCh01G012790 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G012790
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncytochrome P450 CYP72A219-like
LocationCma_Chr01: 9202397 .. 9204828 (-)
RNA-Seq ExpressionCmaCh01G012790
SyntenyCmaCh01G012790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAGAAAGATGGAGGAGGTTAATAGAGAAATAAATAGTATGGTTTGGGGAATTATAAGCAAGAGACAAATGGCTATGGAGGCTGCTCAAGTTTCTAATAATGCTGATCTTCTAGGGATCCTTTTGGAAGAAAATAAGAAAGATGCTGTTGGGATGTCCATGGAAGATATCATTAGTGAGTGCAAGCTTTTTTATTTTGCTGGCCAAGAAACAACCGCCTCGTTGCTTGTTTGGACAATGGTTCTACTTGGTCGGTATTCAGAATGGCAAGATCAAGCAAGAGCGGAAGTGCTCAATGTTTTTGGTGAAGACAAGAAAGTGGATTTCGATGGTTTAAGTCGTCTCAAAGTAGTAAGTAGTATGAGTAATAGCTTTTTCATTTAGTTCAAGAACGTTAAGTGATCCTAGTGCACTTTTTTTATAAGTCATCTCAAAGTAGTATGTAGCTATGGTTTAAGTTTCTCAAGACCAAGAAATCATTTTCTTTTAATAAGAGTGTAAACCTCTCCCCTGGCATCTTAAAGCCTTGGGGGAAGCCCGAAACCTCTCCCCTGGCATCTTAAAGCCTTGAGGGAAGCCCGAAACCGCTCTCCCTAGCGTCTTAAAGCCTTGGGAGAAGTCCAAAACCTCTCTCCCTAGCGTCTTAAAGCCTTGGGAGAAGCCCGAAACCTCTCTCCCTAGCATCTTAAAGCCTACCTCTCCCCCTTAAAGCCTGGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCTTAAAGTCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGTGTCTTTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCTAGTGTCTTTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCTTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCCTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCCTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCCTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCCTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCCTAAAGCCTTGGGAGAAGCCCCAAACCTCTCCCCCTAGCGTCCTAAAGCCTTGGGAGAAGCCCCAAACCTCTCTCCCTAGCGTCCTAAAGCCTTGGGAGAAGCCCCAAACCTCTCTCCCTAGCGTCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCAAAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCATCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCAAAAAGCCTTGGGGAAAGCCCCAAACCTCTCCCTAGCGTCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCAAAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCAAGCGTCCTAAAGCCTTGGGGAAAGCCCCAAACCTCTCTCCCTAGCGTCCTAAAGCCTTGAGGAAAGCCCGAAACCTCTCTCTCCCTAGCGTCTTAAAGCCTTGAGGGAAAGCCCGAAACCTCTCTCTCCCTAGCGTCTCAAGGCCTTGAGGGGAGCCCACCAAAAAAGAAAAACCAAAGAGAGCAATCACTTTAAAGTAGTTTCGATCGGGTATGTTTTGGTTACTTTCTGATATAATTGAACATGTTGATGTTTGGAACAGGTGAGTATGATACTATATGAAGTTCTTAGGTTATATCCTCCCGTTGGGATCTTAGCTCGAGGAATACACAAGGAAACAAAGCTTGGAAACTTATTATTGCCGGGTGGGGTGTCAATAGGTATACCAATCATTCATATCCACCACGATCACCAAATATGGGGTGAAGATGCAGATGAGTTCAAGCCAAAGAGGTTTTCAGAAGGAATTTCCAAAGCAACGAACAATAAAGTTTGCTTCCTACCATTTGGATGGGGTCCTCGAATTTGTGTTGGTCAAAACTTCGCCATGATTGAAGCAAAAATAGCACTATCAATGATTTTGCAGCGCTTCTCCTTCCAACTTTCCCCATATTATACTCACGCCCCTGCTTCAAATGTCACTGTTCAACCACAACATGGAGCTCATCTCATCCTCCGTAAGCTCTAATAGTTTCAAAGTTCATAAATTATGAACTTTTGTGTTGTGTAAGTGTTTGTCTAATATAAATAACTCAATCATTAAGACAATAATATACTTTGATTTGACATATATAAAGTGTGTCCATG

mRNA sequence

ATGAATAGAAAGATGGAGGAGGTTAATAGAGAAATAAATAGTATGGTTTGGGGAATTATAAGCAAGAGACAAATGGCTATGGAGGCTGCTCAAGTTTCTAATAATGCTGATCTTCTAGGGATCCTTTTGGAAGAAAATAAGAAAGATGCTGTTGGGATGTCCATGGAAGATATCATTAGTGAGTGCAAGCTTTTTTATTTTGCTGGCCAAGAAACAACCGCCTCGTTGCTTGTTTGGACAATGGTTCTACTTGGTCGGTATTCAGAATGGCAAGATCAAGCAAGAGCGGAAGTGCTCAATGTTTTTGGTGAAGACAAGAAAGTGGATTTCGATGGTTTAAGTCGTCTCAAAGTAGTGAGTATGATACTATATGAAGTTCTTAGGTTATATCCTCCCGTTGGGATCTTAGCTCGAGGAATACACAAGGAAACAAAGCTTGGAAACTTATTATTGCCGGGTGGGGTGTCAATAGGTATACCAATCATTCATATCCACCACGATCACCAAATATGGGGTGAAGATGCAGATGAGTTCAAGCCAAAGAGGTTTTCAGAAGGAATTTCCAAAGCAACGAACAATAAAGTTTGCTTCCTACCATTTGGATGGGGTCCTCGAATTTGTGTTGGTCAAAACTTCGCCATGATTGAAGCAAAAATAGCACTATCAATGATTTTGCAGCGCTTCTCCTTCCAACTTTCCCCATATTATACTCACGCCCCTGCTTCAAATGTCACTGTTCAACCACAACATGGAGCTCATCTCATCCTCCGTAAGCTCTAATAGTTTCAAAGTTCATAAATTATGAACTTTTGTGTTGTGTAAGTGTTTGTCTAATATAAATAACTCAATCATTAAGACAATAATATACTTTGATTTGACATATATAAAGTGTGTCCATG

Coding sequence (CDS)

ATGAATAGAAAGATGGAGGAGGTTAATAGAGAAATAAATAGTATGGTTTGGGGAATTATAAGCAAGAGACAAATGGCTATGGAGGCTGCTCAAGTTTCTAATAATGCTGATCTTCTAGGGATCCTTTTGGAAGAAAATAAGAAAGATGCTGTTGGGATGTCCATGGAAGATATCATTAGTGAGTGCAAGCTTTTTTATTTTGCTGGCCAAGAAACAACCGCCTCGTTGCTTGTTTGGACAATGGTTCTACTTGGTCGGTATTCAGAATGGCAAGATCAAGCAAGAGCGGAAGTGCTCAATGTTTTTGGTGAAGACAAGAAAGTGGATTTCGATGGTTTAAGTCGTCTCAAAGTAGTGAGTATGATACTATATGAAGTTCTTAGGTTATATCCTCCCGTTGGGATCTTAGCTCGAGGAATACACAAGGAAACAAAGCTTGGAAACTTATTATTGCCGGGTGGGGTGTCAATAGGTATACCAATCATTCATATCCACCACGATCACCAAATATGGGGTGAAGATGCAGATGAGTTCAAGCCAAAGAGGTTTTCAGAAGGAATTTCCAAAGCAACGAACAATAAAGTTTGCTTCCTACCATTTGGATGGGGTCCTCGAATTTGTGTTGGTCAAAACTTCGCCATGATTGAAGCAAAAATAGCACTATCAATGATTTTGCAGCGCTTCTCCTTCCAACTTTCCCCATATTATACTCACGCCCCTGCTTCAAATGTCACTGTTCAACCACAACATGGAGCTCATCTCATCCTCCGTAAGCTCTAA

Protein sequence

MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHAPASNVTVQPQHGAHLILRKL
Homology
BLAST of CmaCh01G012790 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 3.8e-89
Identity = 161/265 (60.75%), Postives = 206/265 (77.74%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDA-------VGMS 61
           NRKM+E++R+  S +  II+K+  AM+A + S + D+LGILLE N K+        VGMS
Sbjct: 258 NRKMKEIDRKTQSCLMNIINKKTKAMQAGEGSTD-DILGILLESNLKEQLGQGKKNVGMS 317

Query: 62  MEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLS 121
           +++++ ECK FYFAGQETT+ LLVWTMVLL  +  WQ +AR EVL  FG + K DFD L+
Sbjct: 318 IQEVMGECKQFYFAGQETTSGLLVWTMVLLSIHPNWQARAREEVLQQFG-NAKPDFDNLN 377

Query: 122 RLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGED 181
            LK+V+MILYEVLRLYPPV  L R + +ET LG++ LP GV I +PI+ +HHD  IWG+D
Sbjct: 378 HLKIVTMILYEVLRLYPPVDTLFRRVDQETTLGDITLPAGVQISLPIMILHHDQNIWGDD 437

Query: 182 ADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSP 241
           A EF P+RFSEG+SKAT N+V F PFGWGPRIC+GQNFA++EAK+AL++ILQRFSF+LSP
Sbjct: 438 AKEFNPERFSEGVSKATKNQVVFFPFGWGPRICIGQNFALLEAKLALAIILQRFSFELSP 497

Query: 242 YYTHAPASNVTVQPQHGAHLILRKL 260
            YTHAP + +TVQPQHGA+LIL KL
Sbjct: 498 SYTHAPTTVLTVQPQHGANLILHKL 520

BLAST of CmaCh01G012790 vs. ExPASy Swiss-Prot
Match: Q9LUC6 (Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 8.0e-87
Identity = 156/260 (60.00%), Postives = 202/260 (77.69%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEEN--KKDAVGMSMEDII 61
           NR+M+   REI  ++ GII+KR+ A E+ +  +  DLLGILLE N  + +  GMS ED++
Sbjct: 255 NRRMKTAAREIQDILRGIINKRERARESGEAPSE-DLLGILLESNLGQTEGNGMSTEDMM 314

Query: 62  SECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVV 121
            ECKLFY AGQETT+ LLVWTMVLL ++ +WQ +AR EV  VFG DK+ D +GL++LKV+
Sbjct: 315 EECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFG-DKQPDTEGLNQLKVM 374

Query: 122 SMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFK 181
           +MILYEVLRLYPPV  L R IHKE KLG+L LPGGV I +P++ +H D ++WG DA EFK
Sbjct: 375 TMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFK 434

Query: 182 PKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHA 241
           P+RF +G+SKAT N+V F PF WGPRIC+GQNF ++EAK+A+S+ILQRFSF+LSP Y HA
Sbjct: 435 PERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHA 494

Query: 242 PASNVTVQPQHGAHLILRKL 260
           P + +T+ PQ GAHL+L KL
Sbjct: 495 PYTIITLYPQFGAHLMLHKL 512

BLAST of CmaCh01G012790 vs. ExPASy Swiss-Prot
Match: H1A988 (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.0e-86
Identity = 156/265 (58.87%), Postives = 203/265 (76.60%), Query Frame = 0

Query: 3   RKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE----ENKKD----AVGMS 62
           R+M+E+ R I   + GII KR+ A+++ + S + DLLGILL+    ENK D    + GM+
Sbjct: 261 RRMKEIERGIRDSLEGIIRKREKALKSGK-STDDDLLGILLQSNHIENKGDENSKSAGMT 320

Query: 63  MEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLS 122
            ++++ ECKLFY AGQETTA+LL WTMVLLG++ EWQ +AR EVL VFG ++  +F+GL 
Sbjct: 321 TQEVMEECKLFYLAGQETTAALLAWTMVLLGKHPEWQARARQEVLQVFG-NQNPNFEGLG 380

Query: 123 RLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGED 182
           RLK+V+MILYEVLRLYPP   L R + K+ KLGNLLLP GV + +PI+ IHHD  IWG D
Sbjct: 381 RLKIVTMILYEVLRLYPPGIYLTRALRKDLKLGNLLLPAGVQVSVPILLIHHDEGIWGND 440

Query: 183 ADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSP 242
           A EF P+RF+EGI+KAT  +VC+ PFGWGPRICVGQNFA++EAKI LS++LQ FSF+LSP
Sbjct: 441 AKEFNPERFAEGIAKATKGQVCYFPFGWGPRICVGQNFALLEAKIVLSLLLQNFSFELSP 500

Query: 243 YYTHAPASNVTVQPQHGAHLILRKL 260
            Y H P + +T+QP+HGA +IL KL
Sbjct: 501 TYAHVPTTVLTLQPKHGAPIILHKL 523

BLAST of CmaCh01G012790 vs. ExPASy Swiss-Prot
Match: H1A981 (11-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula OX=3880 GN=CYP72A63 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 8.8e-86
Identity = 153/264 (57.95%), Postives = 205/264 (77.65%), Query Frame = 0

Query: 4   KMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDA--------VGMSM 63
           KM+E++REI+  + GII KR+ A++  + +N+ DLLGILL+ N  +         +GM+ 
Sbjct: 263 KMKEIDREIHDSLEGIIEKREKALKNGETTND-DLLGILLQSNHAEKQGQGNSKNIGMTT 322

Query: 64  EDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSR 123
           +D+I ECKLFY AGQETT+SLLVWTMVLLGRY EWQ +AR EVL VFG ++  + +GLS+
Sbjct: 323 QDVIDECKLFYLAGQETTSSLLVWTMVLLGRYPEWQARAREEVLQVFG-NQNPNNEGLSQ 382

Query: 124 LKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDA 183
           LK+V+MILYEVLRL+PP+    R + K+ KLGNLLLP G  I +PI+ IH DH +WG+DA
Sbjct: 383 LKIVTMILYEVLRLFPPLIYFNRALRKDLKLGNLLLPEGTQISLPILLIHQDHDLWGDDA 442

Query: 184 DEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPY 243
            EFKP+RF+EGI+KAT  +V + PFGWGPRIC+GQNFA++EAKIA+S++LQ FSF+LSP 
Sbjct: 443 KEFKPERFAEGIAKATKGQVSYFPFGWGPRICLGQNFALLEAKIAVSLLLQNFSFELSPN 502

Query: 244 YTHAPASNVTVQPQHGAHLILRKL 260
           Y H P + +T+QP++GA +IL KL
Sbjct: 503 YVHVPTTVLTLQPKNGASIILHKL 524

BLAST of CmaCh01G012790 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.4e-85
Identity = 151/265 (56.98%), Postives = 204/265 (76.98%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDA-------VGMS 61
           N++M+E+ +E+   +  II+KR  AMEA + S++ DLLGILLE N K+         G++
Sbjct: 254 NKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKEIKQHGNTNFGLT 313

Query: 62  MEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLS 121
           ++++I ECKLF+FAGQETT++LLVWTM+LL ++ +WQ +A+ EVL  FG +K  DFDGL+
Sbjct: 314 VDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNKP-DFDGLN 373

Query: 122 RLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGED 181
            LKVV+MIL EVLRLYPP+  L R I++E KLG + LP GV + +PII +H+D +IWG+D
Sbjct: 374 HLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEIWGDD 433

Query: 182 ADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSP 241
           A EF P+RFSEG+ KAT  +V + PF WGPRIC+GQNFAM+EAK+A++MILQRFSF LSP
Sbjct: 434 AKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSFVLSP 493

Query: 242 YYTHAPASNVTVQPQHGAHLILRKL 260
            Y HAP + +T+QPQ+GAHLIL  L
Sbjct: 494 SYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of CmaCh01G012790 vs. ExPASy TrEMBL
Match: A0A6J1FTW8 (cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111446798 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 251/259 (96.91%), Postives = 255/259 (98.46%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS 60
           MNRKMEEVNREI SMVWGIISKRQ AMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS
Sbjct: 259 MNRKMEEVNREIKSMVWGIISKRQTAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS 318

Query: 61  ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVVS 120
           ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVL+VFGEDKKVDFDGL+RLKVVS
Sbjct: 319 ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLDVFGEDKKVDFDGLNRLKVVS 378

Query: 121 MILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP 180
           MILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP
Sbjct: 379 MILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP 438

Query: 181 KRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHAP 240
           +RFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIAL+MILQ FSFQLSP YTHAP
Sbjct: 439 ERFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALTMILQHFSFQLSPSYTHAP 498

Query: 241 ASNVTVQPQHGAHLILRKL 260
           ASNVTVQPQHGAHLILRKL
Sbjct: 499 ASNVTVQPQHGAHLILRKL 517

BLAST of CmaCh01G012790 vs. ExPASy TrEMBL
Match: A0A6J1KZI7 (cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111499007 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.7e-111
Identity = 203/268 (75.75%), Postives = 234/268 (87.31%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE---------ENKKDAV 60
           +N++M+E+N +I  M+WGIISKRQ AM+  + SN+ DLLGILLE         +NKKD V
Sbjct: 251 LNKRMDEINGKIRDMIWGIISKRQNAMKKGEASND-DLLGILLESNSREIQEHKNKKD-V 310

Query: 61  GMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFD 120
           GMS+E++I+EC+LFYFAGQETTA+LL WTMVLLGRYSEWQD+ARAEVL VFG++KK+DFD
Sbjct: 311 GMSIEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNKKLDFD 370

Query: 121 GLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIW 180
           GLSRLKVV+MIL EVLRLYPPVG+LAR IHKETKLGNL LP GVSIGIPI+ IH D  IW
Sbjct: 371 GLSRLKVVTMILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTIW 430

Query: 181 GEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQ 240
           GEDA EFKP+RF+EGISKAT N+VCF+PFGWGPRIC+GQNFAMIEAKIALSMILQRFSF+
Sbjct: 431 GEDASEFKPERFAEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQRFSFE 490

Query: 241 LSPYYTHAPASNVTVQPQHGAHLILRKL 260
           LSP YTHAP SNVT+QPQHGAHLIL  L
Sbjct: 491 LSPSYTHAPISNVTIQPQHGAHLILHML 516

BLAST of CmaCh01G012790 vs. ExPASy TrEMBL
Match: A0A6J1EFS6 (cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432119 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 4.1e-110
Identity = 199/268 (74.25%), Postives = 232/268 (86.57%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE---------ENKKDAV 60
           +N++M+E+N  I  M+WGII+KRQ AM+  + SN+ DLLGILLE         +NKKD V
Sbjct: 273 LNKRMDEINGNIRDMIWGIINKRQNAMKKGEASND-DLLGILLESNSREIQEHKNKKD-V 332

Query: 61  GMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFD 120
           GMSME++I+EC+LFYFAGQETTA+LL WTMVLLGRYSEWQD+ARAEVL +FG++KK DFD
Sbjct: 333 GMSMEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNKKFDFD 392

Query: 121 GLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIW 180
           GLSRLK+V+MIL EVLRLYPPVG+L+R IHKETKLGNL LP GVSIGIPI+ IH D  +W
Sbjct: 393 GLSRLKIVTMILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTLW 452

Query: 181 GEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQ 240
           GEDA EFKP+RF EGISKAT N+VCF+PFGWGPRIC+GQNFAMIEAKIALSMILQRFSF+
Sbjct: 453 GEDASEFKPERFVEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQRFSFE 512

Query: 241 LSPYYTHAPASNVTVQPQHGAHLILRKL 260
           LSP YTHAP +NVT+QPQHGAHLILR L
Sbjct: 513 LSPSYTHAPITNVTIQPQHGAHLILRML 538

BLAST of CmaCh01G012790 vs. ExPASy TrEMBL
Match: A0A6J1E9Z3 (cytochrome P450 CYP72A219-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432119 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.7e-109
Identity = 198/268 (73.88%), Postives = 231/268 (86.19%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE---------ENKKDAV 60
           +N++M+E+N  I  M+WGII+KRQ AM+  + SN+ DLLGILLE         +NKKD V
Sbjct: 273 LNKRMDEINGNIRDMIWGIINKRQNAMKKGEASND-DLLGILLESNSREIQEHKNKKD-V 332

Query: 61  GMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFD 120
           GMSME++I+EC+LFYFAGQETTA+LL WTMVLLGRYSEWQD+ARAEVL +FG++KK DFD
Sbjct: 333 GMSMEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNKKFDFD 392

Query: 121 GLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIW 180
           GLSRLK+V+MIL EVLRLYPPVG+L+R IHKETKLGNL LP GVSIGIPI+ IH D  +W
Sbjct: 393 GLSRLKIVTMILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTLW 452

Query: 181 GEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQ 240
           GEDA EFKP+RF EGISKAT N+VCF+PFGWGPRIC+GQNFAMIEAKIALSMILQRFSF+
Sbjct: 453 GEDASEFKPERFVEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQRFSFE 512

Query: 241 LSPYYTHAPASNVTVQPQHGAHLILRKL 260
           LSP YTHAP +NVT+QPQHGAHLIL  L
Sbjct: 513 LSPSYTHAPITNVTIQPQHGAHLILPML 538

BLAST of CmaCh01G012790 vs. ExPASy TrEMBL
Match: A0A5A7UEV9 (Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001490 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.5e-107
Identity = 195/268 (72.76%), Postives = 229/268 (85.45%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE---------ENKKDAV 60
           +N++M+E++ +I  +V GII+KRQ AM+  + S N DLLGILLE         +NKKD V
Sbjct: 78  LNKRMQEIDMKIRDLVRGIINKRQNAMKKGEASKNEDLLGILLESNETQIEEQKNKKD-V 137

Query: 61  GMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFD 120
           GMS+E++ISEC+LFYFAGQETTA LL WTMVLLGRYSEWQD+ARAEVL VFG +KK+DFD
Sbjct: 138 GMSIEEVISECRLFYFAGQETTAVLLAWTMVLLGRYSEWQDRARAEVLEVFGNNKKLDFD 197

Query: 121 GLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIW 180
           GLSRL+VVSMIL EVLRLYPPVG+LAR +H ETKLGNL LP GVSIGIPI+ +H + +IW
Sbjct: 198 GLSRLRVVSMILNEVLRLYPPVGMLAREVHNETKLGNLTLPSGVSIGIPILSMHQNPKIW 257

Query: 181 GEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQ 240
           GED  EF P+RFSEGISKAT N+VCF+PFGWGPRIC+GQNFAMIEAKIALSMILQ+FSF 
Sbjct: 258 GEDVLEFNPERFSEGISKATKNQVCFIPFGWGPRICIGQNFAMIEAKIALSMILQQFSFT 317

Query: 241 LSPYYTHAPASNVTVQPQHGAHLILRKL 260
           LSP YTHAP +N+T+QPQHGAHLILRKL
Sbjct: 318 LSPTYTHAPITNITIQPQHGAHLILRKL 344

BLAST of CmaCh01G012790 vs. NCBI nr
Match: XP_022941520.1 (cytochrome P450 CYP72A219-like [Cucurbita moschata])

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 251/259 (96.91%), Postives = 255/259 (98.46%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS 60
           MNRKMEEVNREI SMVWGIISKRQ AMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS
Sbjct: 259 MNRKMEEVNREIKSMVWGIISKRQTAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS 318

Query: 61  ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVVS 120
           ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVL+VFGEDKKVDFDGL+RLKVVS
Sbjct: 319 ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLDVFGEDKKVDFDGLNRLKVVS 378

Query: 121 MILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP 180
           MILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP
Sbjct: 379 MILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP 438

Query: 181 KRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHAP 240
           +RFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIAL+MILQ FSFQLSP YTHAP
Sbjct: 439 ERFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALTMILQHFSFQLSPSYTHAP 498

Query: 241 ASNVTVQPQHGAHLILRKL 260
           ASNVTVQPQHGAHLILRKL
Sbjct: 499 ASNVTVQPQHGAHLILRKL 517

BLAST of CmaCh01G012790 vs. NCBI nr
Match: XP_023524668.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.8 bits (1301), Expect = 2.3e-139
Identity = 248/259 (95.75%), Postives = 255/259 (98.46%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS 60
           MNRKMEEVNREI SMVWGIISKRQ AMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS
Sbjct: 259 MNRKMEEVNREIKSMVWGIISKRQAAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIIS 318

Query: 61  ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVVS 120
           ECKLFYFAGQETTASLLVWTMVLLGRYSEWQD+ARAEVL+VFGEDKKVDFDGL+RLKVVS
Sbjct: 319 ECKLFYFAGQETTASLLVWTMVLLGRYSEWQDRARAEVLDVFGEDKKVDFDGLNRLKVVS 378

Query: 121 MILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP 180
           MILYEVLRLYPPVGILARGIHKETKLGNL+LPGGVS+GIPIIHIHHDHQIWGEDADEFKP
Sbjct: 379 MILYEVLRLYPPVGILARGIHKETKLGNLILPGGVSLGIPIIHIHHDHQIWGEDADEFKP 438

Query: 181 KRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHAP 240
           +RFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQ FSFQLSP YTH+P
Sbjct: 439 ERFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQSFSFQLSPSYTHSP 498

Query: 241 ASNVTVQPQHGAHLILRKL 260
           ASNVTVQPQHGAHLILRKL
Sbjct: 499 ASNVTVQPQHGAHLILRKL 517

BLAST of CmaCh01G012790 vs. NCBI nr
Match: XP_023006210.1 (cytochrome P450 CYP72A219-like [Cucurbita maxima])

HSP 1 Score: 411.0 bits (1055), Expect = 7.6e-111
Identity = 203/268 (75.75%), Postives = 234/268 (87.31%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE---------ENKKDAV 60
           +N++M+E+N +I  M+WGIISKRQ AM+  + SN+ DLLGILLE         +NKKD V
Sbjct: 251 LNKRMDEINGKIRDMIWGIISKRQNAMKKGEASND-DLLGILLESNSREIQEHKNKKD-V 310

Query: 61  GMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFD 120
           GMS+E++I+EC+LFYFAGQETTA+LL WTMVLLGRYSEWQD+ARAEVL VFG++KK+DFD
Sbjct: 311 GMSIEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNKKLDFD 370

Query: 121 GLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIW 180
           GLSRLKVV+MIL EVLRLYPPVG+LAR IHKETKLGNL LP GVSIGIPI+ IH D  IW
Sbjct: 371 GLSRLKVVTMILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTIW 430

Query: 181 GEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQ 240
           GEDA EFKP+RF+EGISKAT N+VCF+PFGWGPRIC+GQNFAMIEAKIALSMILQRFSF+
Sbjct: 431 GEDASEFKPERFAEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQRFSFE 490

Query: 241 LSPYYTHAPASNVTVQPQHGAHLILRKL 260
           LSP YTHAP SNVT+QPQHGAHLIL  L
Sbjct: 491 LSPSYTHAPISNVTIQPQHGAHLILHML 516

BLAST of CmaCh01G012790 vs. NCBI nr
Match: XP_038904026.1 (cytochrome P450 CYP72A219-like [Benincasa hispida])

HSP 1 Score: 410.2 bits (1053), Expect = 1.3e-110
Identity = 202/268 (75.37%), Postives = 232/268 (86.57%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE---------ENKKDAV 60
           +N++MEE++R+I  MVWGII KRQ AM+  + SNN DLLGILLE         +NKKD V
Sbjct: 255 LNKRMEEIDRKIQDMVWGIIRKRQNAMKKGEGSNNEDLLGILLESNASQIEEHKNKKD-V 314

Query: 61  GMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFD 120
           GMS+E++ISEC+LFYFAGQETTA+LL WTMVLL RYSEWQD+ARAEVL VFG+  K+DFD
Sbjct: 315 GMSIEEVISECRLFYFAGQETTAALLAWTMVLLARYSEWQDRARAEVLEVFGDSNKLDFD 374

Query: 121 GLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIW 180
           GLSRL+VV+MIL EVLRLYPPVG+LAR IH ETKLGNL LP GVSIGIPI+ IH + +IW
Sbjct: 375 GLSRLRVVNMILNEVLRLYPPVGMLAREIHNETKLGNLTLPVGVSIGIPILSIHQNTKIW 434

Query: 181 GEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQ 240
           GEDA EFKP+RFSEGISKAT N+VCF+PFGWGPRIC+GQNFAMIEAKIALSMILQ+FSF 
Sbjct: 435 GEDAIEFKPERFSEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQQFSFT 494

Query: 241 LSPYYTHAPASNVTVQPQHGAHLILRKL 260
           LSP YTHAP SN+T+QPQHGAHLILRKL
Sbjct: 495 LSPAYTHAPISNITIQPQHGAHLILRKL 521

BLAST of CmaCh01G012790 vs. NCBI nr
Match: XP_023529493.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 409.5 bits (1051), Expect = 2.2e-110
Identity = 201/268 (75.00%), Postives = 233/268 (86.94%), Query Frame = 0

Query: 1   MNRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLE---------ENKKDAV 60
           +N++M+E+N  I  M+WGII+KRQ AM+  + SN+ DLLGILLE         +NKKD V
Sbjct: 251 LNKRMDEINGNIRDMIWGIINKRQNAMKKGEASND-DLLGILLESNSREIQEHKNKKD-V 310

Query: 61  GMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFD 120
           GMS+E++I+EC+LFYFAGQETTA+LL WTMVLLGRYSEWQD+ARAEVL VFG++KK DFD
Sbjct: 311 GMSIEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNKKFDFD 370

Query: 121 GLSRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIW 180
           GLSRLK+V+MIL EVLRLYPPVG+LAR IHKETKLGNL LP GVSIGIPI+ IH D  IW
Sbjct: 371 GLSRLKIVTMILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTIW 430

Query: 181 GEDADEFKPKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQ 240
           GEDA EFKP+RF+EGISKAT N+VCF+PFGWGPRIC+GQNFAMIEAKIALSMILQRFSF+
Sbjct: 431 GEDASEFKPERFAEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQRFSFE 490

Query: 241 LSPYYTHAPASNVTVQPQHGAHLILRKL 260
           LSP YTHAP SNVT+QPQHGAHLIL +L
Sbjct: 491 LSPSYTHAPISNVTIQPQHGAHLILHRL 516

BLAST of CmaCh01G012790 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 321.6 bits (823), Expect = 5.7e-88
Identity = 156/260 (60.00%), Postives = 202/260 (77.69%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEEN--KKDAVGMSMEDII 61
           NR+M+   REI  ++ GII+KR+ A E+ +  +  DLLGILLE N  + +  GMS ED++
Sbjct: 255 NRRMKTAAREIQDILRGIINKRERARESGEAPSE-DLLGILLESNLGQTEGNGMSTEDMM 314

Query: 62  SECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVV 121
            ECKLFY AGQETT+ LLVWTMVLL ++ +WQ +AR EV  VFG DK+ D +GL++LKV+
Sbjct: 315 EECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFG-DKQPDTEGLNQLKVM 374

Query: 122 SMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFK 181
           +MILYEVLRLYPPV  L R IHKE KLG+L LPGGV I +P++ +H D ++WG DA EFK
Sbjct: 375 TMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFK 434

Query: 182 PKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHA 241
           P+RF +G+SKAT N+V F PF WGPRIC+GQNF ++EAK+A+S+ILQRFSF+LSP Y HA
Sbjct: 435 PERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHA 494

Query: 242 PASNVTVQPQHGAHLILRKL 260
           P + +T+ PQ GAHL+L KL
Sbjct: 495 PYTIITLYPQFGAHLMLHKL 512

BLAST of CmaCh01G012790 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 319.7 bits (818), Expect = 2.2e-87
Identity = 151/260 (58.08%), Postives = 204/260 (78.46%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDAV--GMSMEDII 61
           NR+M+ ++RE++ ++ GI+SKR+ A EA + +N+ DLLGILLE N +++   GMS+ED++
Sbjct: 254 NRRMKAIDREVDVILRGIVSKREKAREAGEPAND-DLLGILLESNSEESQGNGMSVEDVM 313

Query: 62  SECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVV 121
            ECKLFYFAGQETT+ LLVWTMVLL  + +WQ +AR EV+ V GE+ K D + L+ LKV+
Sbjct: 314 KECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPDMESLNNLKVM 373

Query: 122 SMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFK 181
           +MI  EVLRLYPPV  L R ++KE KLG L LP G+ I +P I +  D ++WG+DA +FK
Sbjct: 374 TMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTELWGDDAADFK 433

Query: 182 PKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHA 241
           P+RF +G+SKAT N+V F PFGWGPRIC+GQNFAM+EAK+A+++ILQ+FSF+LSP Y HA
Sbjct: 434 PERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALILQKFSFELSPSYVHA 493

Query: 242 PASNVTVQPQHGAHLILRKL 260
           P + +T +PQ GAHLIL KL
Sbjct: 494 PQTVMTTRPQFGAHLILHKL 512

BLAST of CmaCh01G012790 vs. TAIR 10
Match: AT3G14630.1 (cytochrome P450, family 72, subfamily A, polypeptide 9 )

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-86
Identity = 150/260 (57.69%), Postives = 205/260 (78.85%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDAV--GMSMEDII 61
           NR+M+ + +EI  ++ GIIS R+ A +A +  ++ DLLGILL+ N + +   G++ME+I+
Sbjct: 251 NRRMKTIVKEIQVILRGIISHREKARDAGEAPSD-DLLGILLKSNSEQSKGNGLNMEEIM 310

Query: 62  SECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVV 121
            ECKLFYFAGQETT+ LL WTMVLL ++ +WQ +AR EV+ VFG +K  D  G+++LKV+
Sbjct: 311 EECKLFYFAGQETTSVLLAWTMVLLSQHQDWQARAREEVMQVFGHNKP-DLQGINQLKVM 370

Query: 122 SMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFK 181
           +MI+YEVLRLYPPV  + R  HKE KLG++ LPGG+ + +P++ IH D ++WG+DA EFK
Sbjct: 371 TMIIYEVLRLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRDTKLWGDDAAEFK 430

Query: 182 PKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHA 241
           P+RF +GI+KAT N+VCFLPFGWGPRIC+GQNFA++EAK+AL++ILQRFSF+LSP Y H+
Sbjct: 431 PERFKDGIAKATKNQVCFLPFGWGPRICIGQNFALLEAKMALALILQRFSFELSPSYVHS 490

Query: 242 PASNVTVQPQHGAHLILRKL 260
           P    T+ PQ GAHLIL KL
Sbjct: 491 PYRVFTIHPQCGAHLILHKL 508

BLAST of CmaCh01G012790 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 315.1 bits (806), Expect = 5.3e-86
Identity = 153/260 (58.85%), Postives = 201/260 (77.31%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEEN--KKDAVGMSMEDII 61
           NR+M+   REI  ++ GI++KR  A EA +  ++ DLLGILLE N  + +  GMS ED++
Sbjct: 255 NRRMKAAAREIQVILRGIVNKRLRAREAGEAPSD-DLLGILLESNLRQTEGNGMSTEDLM 314

Query: 62  SECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVV 121
            ECKLFYFAGQETT+ LLVWTMVLL ++ +WQ +AR EV  VFG DK+ D +GL++LKV+
Sbjct: 315 EECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFG-DKEPDAEGLNQLKVM 374

Query: 122 SMILYEVLRLYPPVGILARGIHKETKLGNLLLPGGVSIGIPIIHIHHDHQIWGEDADEFK 181
           +MILYEVLRLYPPV  L R IHKE KLG+L LPGGV I +PI+ + HD ++WG DA EF 
Sbjct: 375 TMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGNDAAEFN 434

Query: 182 PKRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHA 241
           P RF +G+SKAT ++V F PF WGPRIC+GQNFA++EAK+A+++IL+RFSF++SP Y HA
Sbjct: 435 PDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSFEISPSYVHA 494

Query: 242 PASNVTVQPQHGAHLILRKL 260
           P + +T+ PQ GA LI+ KL
Sbjct: 495 PYTVITIHPQFGAQLIMHKL 512

BLAST of CmaCh01G012790 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 314.3 bits (804), Expect = 9.1e-86
Identity = 152/259 (58.69%), Postives = 196/259 (75.68%), Query Frame = 0

Query: 2   NRKMEEVNREINSMVWGIISKRQMAMEAAQVSNNADLLGILLEENKKDAVGMSMEDIISE 61
           N +M ++N+E+ S +  II KRQ  M+  +   N DLLGILLE N  D  GMS+ED++ E
Sbjct: 259 NLRMRQINKEVKSRLREIIMKRQRGMDTGEAPKN-DLLGILLESNSGDH-GMSIEDVVEE 318

Query: 62  CKLFYFAGQETTASLLVWTMVLLGRYSEWQDQARAEVLNVFGEDKKVDFDGLSRLKVVSM 121
           C+LF+FAGQETTA LLVWTM++L  + +WQDQAR E+L V G++ K +FD LSRLK +SM
Sbjct: 319 CRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEILKVIGKNNKPNFDALSRLKTMSM 378

Query: 122 ILYEVLRLYPPVGILARGIHKETKLG-NLLLPGGVSIGIPIIHIHHDHQIWGEDADEFKP 181
           IL EVLRLYPP  +L R + KETKLG ++ LPGG  + IP++ +H D ++WGED  EF P
Sbjct: 379 ILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVVIPVLMVHRDPELWGEDVHEFNP 438

Query: 182 KRFSEGISKATNNKVCFLPFGWGPRICVGQNFAMIEAKIALSMILQRFSFQLSPYYTHAP 241
           +RF++GISKAT N+V FLPFGWGPR C GQNFA++EAK+AL +ILQRFSF+LSP YTHAP
Sbjct: 439 ERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEAKMALVLILQRFSFELSPSYTHAP 498

Query: 242 ASNVTVQPQHGAHLILRKL 260
            + +T+ PQ GA LI   L
Sbjct: 499 HTVLTLHPQFGAPLIFHML 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A0S2IHL23.8e-8960.75Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1[more]
Q9LUC68.0e-8760.00Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1[more]
H1A9883.0e-8658.8711-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=... [more]
H1A9818.8e-8657.9511-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula OX=3880 GN=CYP72A63 PE=1 SV... [more]
H2DH213.4e-8556.98Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FTW81.0e-14096.91cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111446798 PE=... [more]
A0A6J1KZI73.7e-11175.75cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111499007 PE=3 ... [more]
A0A6J1EFS64.1e-11074.25cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1E9Z32.7e-10973.88cytochrome P450 CYP72A219-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5A7UEV91.5e-10772.76Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
XP_022941520.12.1e-14096.91cytochrome P450 CYP72A219-like [Cucurbita moschata][more]
XP_023524668.12.3e-13995.75cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo][more]
XP_023006210.17.6e-11175.75cytochrome P450 CYP72A219-like [Cucurbita maxima][more]
XP_038904026.11.3e-11075.37cytochrome P450 CYP72A219-like [Benincasa hispida][more]
XP_023529493.12.2e-11075.00cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G14680.15.7e-8860.00cytochrome P450, family 72, subfamily A, polypeptide 14 [more]
AT3G14610.12.2e-8758.08cytochrome P450, family 72, subfamily A, polypeptide 7 [more]
AT3G14630.11.1e-8657.69cytochrome P450, family 72, subfamily A, polypeptide 9 [more]
AT3G14690.15.3e-8658.85cytochrome P450, family 72, subfamily A, polypeptide 15 [more]
AT3G14620.19.1e-8658.69cytochrome P450, family 72, subfamily A, polypeptide 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 198..207
score: 61.51
coord: 68..85
score: 39.27
coord: 121..132
score: 38.62
coord: 207..218
score: 39.18
IPR001128Cytochrome P450PFAMPF00067p450coord: 1..234
e-value: 1.0E-58
score: 199.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 57..74
score: 26.77
coord: 77..103
score: 26.57
coord: 207..230
score: 33.33
coord: 197..207
score: 56.46
coord: 120..138
score: 20.81
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..259
e-value: 5.5E-77
score: 261.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..257
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 1..250
NoneNo IPR availablePANTHERPTHR24282:SF12CYTOCHROME P450 72A11-RELATEDcoord: 1..250
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 200..209

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G012790.1CmaCh01G012790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen