CmaCh01G012350 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G012350
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionsodium/calcium exchanger NCL-like
LocationCma_Chr01: 8895999 .. 8900210 (+)
RNA-Seq ExpressionCmaCh01G012350
SyntenyCmaCh01G012350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAAAATCATTTAGAATTTTCAACTTTTAATAATTAAATTAAATTAAATTCTTTCATCTTCCTCGCGGAGAAGATCGCCCTCCTCACATGGAAGCACTGTAAGCCCCACAGGTTCAGTCTCAAAATCTCTCTGTCACAAACCAATCGATCCTTCTTTTAAGCTAATCCCACAGCTCTGTTCTTCAAACCCACTTCATCTCTCTCGCTGCACCTCCAGATTTTGCTCCCATAGCCATGTCCAGACCTCCTCTGTATCTCATCTTCCTCCTCATTTTCTTCCTCCTCTGTGGTCAATCCAACAGCAGTCGATTTTCTTCTATTTCTTCTTCCTCTGATCTGGTCTCCGATGGCGTCAATGAATTGCAGGAAAATTTGCGGCTTAATTCGTTGGAATCTGTCTCTACGCCGGAGGAATCTTGTGAGGAGTCTTATGGGTTTCTTCCATGTACTACCTCTGCTTTGGGGAATCTGTTCTTGATTATCGTTTATGGGTATTTGATGTTCTTGGCTGCTAAGTATTTGTCTACTGGAAGCGAGCTCTTGTTGGAGATTTTGGGACCTGGAATTGTTGGGGGATTGTTTCTTCCTGCGCTTGGTGCTCTTCCTGATGCTATGCTCATTCTTGGTACTTGTTGCTTACTTTTTCACTTTGATGGGTTTTTGAAGCATGATTTCTAATATGGTAGATCACTGGGATTTGTGGAAAATGATTGATAGATCTGCATTTTACTTTGCTTTTCCATTTGTTCATCTTCATACGACCTGTTTGATCGCAGAAATTGACCCGAAAATAAATCAGGAATTTGTCTTTTGTTTATCTTGGCTACTTTTTCAAAGGTTTCGGTAATTGACCCGAAAAAGTGTTTGTTTAAAGTCTCATTTGCTGCTATACTTCTACTTTGTTGGGTTTGAATTTGCTTGGCTCGCGGATCTCTCTGCTTTTGCTAATGCTCACTCTTGTTGCAATTTGTTGTTGATATCAACTTTATCGTACTTCAACTTTTGCTGATTTGATTTAAACCTCGTTGATCAGACCTCAACATCAAGAACCTGAATGATTTTCATCCCTTTTTCTTGAACCGGGATATTTCAAAAGTGTAGCCCTTAGATTCCACACTGGGCACGATTTTTTCCAAAACCTCGTTCATCGAATCGGTTGGAGAGGGACACGAAACATTTCTTGTGAGGGTGTAGAAAATTCTCTCCAACAGACATGTTTTAGAAACCTTGAGAGGAAGCCGGAAGAGAAAGACCAAAGAGAACAATATCTGCGGTGGGCTTGGGCTGTTACAAATGGTACCAGACTCAAACACCGAGCGGTGTGCCAACGAGGATGTTGGACTCCCAAGAGGGTGGATTATGAGATTCTACATTGGTTGGAGAGGTGTACGAAACATTTCTTATGAGGGTGTGGACACCCTCTCCCAATAGACGCGTTTTAAAAACTGTGAGAAAGCCCAAAAGGGAAAGCTCAAAGCTCAAAGAGGACAATATCACTAGTTGTTACAAGTCTAATATCACCATCGATGCATTATTTCAGATAACCCTCTAATCTTCTTTTTTCTATAGTTTCTGGTCTTGCAGGAAGTGCTGAAGTCGCACAAAGCCAGGTTTCTGTGGGAATGGGCCTCTTAGCTGGTTCAACTGTCATGCTGCTCACACTCATCTGGGGCACATGTGTGATTGTTGGCAAGTGTGACCTTCAGGATTCAGTTGCCATAGATTTACAGGATACAAAAGGATTTAGCTTGACTGGTATGTCATTTTGCTTTCATGCAATAAGCCATTGTCATATGCTTTCATTAATATATCCGAAGAAGTTCCGAAGTGTCTGTTTGTGTTCCTGTATGCCTCGTGTGCACGCTACGACTATTTTCACGGGACAATTACTTGGCTGTGTTACATTTGGTTTGTGTTTTTTATGTTGTTCAAACAAGGATGATGGCATTTGTAATGGGTAATCAAGATCTACAAAGATAAGGACATATTTTTTTACATTGTAATTTGTTCTTTGGCAGAATCTGGAGTGAGCACTGATATTTGGACCAGCTATGCTGCCAGGATTATGGTTATATCTGTTGTTCCATTTTTAGTAGTTCAACTTCCACAATTGCTCAATTCAACTTCAGGAAGGCACTTGGCTGTGTTGATTGCTCTTATTATCTCTGTTTCAATGTTCATTATATATTGCCTTTACCAGGTGCGTTCTCTATTTCTTATCGTCTTTTTACGGTTTACTCGACAATTTATTTTCGTCTTTTGGAACTATTTGTCCATACATGGGGAGTAGAACAAGAGCTGAAACGTGAAGGAGGTAAGAGAAGCCGAGTTGGAGAGGTTAACAAGGAGTAATAGTTTTCGCTTTTATTTCTCTTTTATTCTTTCACGAGGTAAATAGGTGAAAAAGCAAATAGACTCTGTCTTCGTCTTTTGACTTTAACTCATTGTCCATAGTAGAGGTCTTTATAACCGTAATTCTAACTCAAATGAGCTTACCTTCCCTTCAAACGTCTTGAATTCGGTGAGCAAGAAACATTGGCGGCTATTCTAGTCTTCCCGAGTCGAAACATTTAGCTGCAACTGAAGGGTCAAGACCTTTACAGTGTACTGGCGTGCTAAGAAACTTGATATCCACTTCATAAGTTGAAAAAATTTGTGCAGGTTTTCCAGCCATGGATTCAAAGGAGGAAACTTGCCTTTGTCAAGCACAAGCATGTTATATTCGGGCTCTTAAAACATTTAAAACAGCAAGCACTTGGAAGACTCCTGACAGAAAGTGGGGAGCCAGATAGAGAAATAATAAAAAAGTTAGTTCGTTGACACAATAAGTTCAATCTTTCATTTTGATCATTGCCTTACATCAGATTCTGATTGCTGCTCAATGAACATTCATGACAGGCTATTTTCAAGAATCGATGCGAATAAGAATGGACTTCTTTCAGCTTCTGAACTGAGAGCATTAATCGTTGGCATTCAGTTTGAGGCGATCGACTTAGATCATGATGATGCTGTTAACAAAATATTGAGTGACTTCGATACTTCTCGTGATAACCATGTCGATTCTATCGAGTTTGCTAATGGGATCATTAGGTGGCTAGCTCAAGTACAAGGAGCAAGAACTGGCAGAGCTGATGATGGTCCACACACATTGAAATACCTGCACAACTTTCATCAAGTAAGTTGTAACCTGGTCGGCGAGCGCTGTAATTTTCCTCTTGATTCTTACCGTCACGTTTCTGAAGCATGACATGATGTATATATAGGAAACAAAGAGAGAGCATGATTTGCTGGATGTCGGGGAACAGAGTGACGAGGTTGTAGAGGGTGTCGAGGAGAGCAAGGGAGTGTTGATCAGAGCCATACTCTTCTTGTTGCTTGGAACAGCCATTGCTGCTGCCTTTGCCGATCCTTTGGTCGACGTTGTACATAACTTCTCCAATGCAACGAGTATCCCTGCGTTCTTCATTTCGTTTATAGCTTTGCCTTTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACCGCCTCCTTAACATTTTCTGAGGTTTGTTCTTTCTTATATACTTAATATTGAAGTGTGCTGTGTGGGGAAGAATGATTGATATCTAAGCTTCCTTGGTGCAGTTATATGGAGCAGTGACAATGAACAATGTGCTTTGTCTATCTGTGTTCTTGGCTCTTGTTTATCTGAGAGGATTGGTTTGGAACTTTTCTTCTGAAGTATTGGTTATTCTGGTCGTTACGATGATCATGGGACTGCTCGGGAGCTTCCGAACCGCGTTCCCGCTGTGGACAGCCCTGGTGGCTCTCCTTCTCTACCCCCTGTCTCTTGTGCTGGTCTATGTCCTTGATTATGTGTTTGGTTGGTCATAGATGAGTTGGTGTGTTTGAGTTATATATGTTCACAGGAGGTTTGTTGATCTAAATTTCCGGATGTTTTAGACACTCTGCTGAGGCTGTGTTAGACACACTCTACTGTTCTGCTCTCTCTTCTGCTGAGGCTGTGTTAGACACACTCTACTGTTCTGCTCTCTCTTTTATTTGCTTTATTGTTCCTACTTTTGTCAATATACGACCGACGTCTTTGATCACGGTGATATATAACGAGTCAAAATAGACACATATCTATTAGTGGTGAACTAAAATTGTGAGGTTGACGACAATATATA

mRNA sequence

AAAAAAAAAAAAAAAAAAAAATCATTTAGAATTTTCAACTTTTAATAATTAAATTAAATTAAATTCTTTCATCTTCCTCGCGGAGAAGATCGCCCTCCTCACATGGAAGCACTGTAAGCCCCACAGGTTCAGTCTCAAAATCTCTCTGTCACAAACCAATCGATCCTTCTTTTAAGCTAATCCCACAGCTCTGTTCTTCAAACCCACTTCATCTCTCTCGCTGCACCTCCAGATTTTGCTCCCATAGCCATGTCCAGACCTCCTCTGTATCTCATCTTCCTCCTCATTTTCTTCCTCCTCTGTGGTCAATCCAACAGCAGTCGATTTTCTTCTATTTCTTCTTCCTCTGATCTGGTCTCCGATGGCGTCAATGAATTGCAGGAAAATTTGCGGCTTAATTCGTTGGAATCTGTCTCTACGCCGGAGGAATCTTGTGAGGAGTCTTATGGGTTTCTTCCATGTACTACCTCTGCTTTGGGGAATCTGTTCTTGATTATCGTTTATGGGTATTTGATGTTCTTGGCTGCTAAGTATTTGTCTACTGGAAGCGAGCTCTTGTTGGAGATTTTGGGACCTGGAATTGTTGGGGGATTGTTTCTTCCTGCGCTTGGTGCTCTTCCTGATGCTATGCTCATTCTTGTTTCTGGTCTTGCAGGAAGTGCTGAAGTCGCACAAAGCCAGGTTTCTGTGGGAATGGGCCTCTTAGCTGGTTCAACTGTCATGCTGCTCACACTCATCTGGGGCACATGTGTGATTGTTGGCAAGTGTGACCTTCAGGATTCAGTTGCCATAGATTTACAGGATACAAAAGGATTTAGCTTGACTGAATCTGGAGTGAGCACTGATATTTGGACCAGCTATGCTGCCAGGATTATGGTTATATCTGTTGTTCCATTTTTAGTAGTTCAACTTCCACAATTGCTCAATTCAACTTCAGGAAGGCACTTGGCTGTGTTGATTGCTCTTATTATCTCTGTTTCAATGTTCATTATATATTGCCTTTACCAGGTTTTCCAGCCATGGATTCAAAGGAGGAAACTTGCCTTTGTCAAGCACAAGCATGTTATATTCGGGCTCTTAAAACATTTAAAACAGCAAGCACTTGGAAGACTCCTGACAGAAAGTGGGGAGCCAGATAGAGAAATAATAAAAAAGCTATTTTCAAGAATCGATGCGAATAAGAATGGACTTCTTTCAGCTTCTGAACTGAGAGCATTAATCGTTGGCATTCAGTTTGAGGCGATCGACTTAGATCATGATGATGCTGTTAACAAAATATTGAGTGACTTCGATACTTCTCGTGATAACCATGTCGATTCTATCGAGTTTGCTAATGGGATCATTAGGTGGCTAGCTCAAGTACAAGGAGCAAGAACTGGCAGAGCTGATGATGGTCCACACACATTGAAATACCTGCACAACTTTCATCAAGAAACAAAGAGAGAGCATGATTTGCTGGATGTCGGGGAACAGAGTGACGAGGTTGTAGAGGGTGTCGAGGAGAGCAAGGGAGTGTTGATCAGAGCCATACTCTTCTTGTTGCTTGGAACAGCCATTGCTGCTGCCTTTGCCGATCCTTTGGTCGACGTTGTACATAACTTCTCCAATGCAACGAGTATCCCTGCGTTCTTCATTTCGTTTATAGCTTTGCCTTTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACCGCCTCCTTAACATTTTCTGAGTTATATGGAGCAGTGACAATGAACAATGTGCTTTGTCTATCTGTGTTCTTGGCTCTTGTTTATCTGAGAGGATTGGTTTGGAACTTTTCTTCTGAAGTATTGGTTATTCTGGTCGTTACGATGATCATGGGACTGCTCGGGAGCTTCCGAACCGCGTTCCCGCTGTGGACAGCCCTGGTGGCTCTCCTTCTCTACCCCCTGTCTCTTGTGCTGGTCTATGTCCTTGATTATGTGTTTGGTTGGTCATAGATGAGTTGGTGTGTTTGAGTTATATATGTTCACAGGAGGTTTGTTGATCTAAATTTCCGGATGTTTTAGACACTCTGCTGAGGCTGTGTTAGACACACTCTACTGTTCTGCTCTCTCTTTTATTTGCTTTATTGTTCCTACTTTTGTCAATATACGACCGACGTCTTTGATCACGGTGATATATAACGAGTCAAAATAGACACATATCTATTAGTGGTGAACTAAAATTGTGAGGTTGACGACAATATATA

Coding sequence (CDS)

ATGTCCAGACCTCCTCTGTATCTCATCTTCCTCCTCATTTTCTTCCTCCTCTGTGGTCAATCCAACAGCAGTCGATTTTCTTCTATTTCTTCTTCCTCTGATCTGGTCTCCGATGGCGTCAATGAATTGCAGGAAAATTTGCGGCTTAATTCGTTGGAATCTGTCTCTACGCCGGAGGAATCTTGTGAGGAGTCTTATGGGTTTCTTCCATGTACTACCTCTGCTTTGGGGAATCTGTTCTTGATTATCGTTTATGGGTATTTGATGTTCTTGGCTGCTAAGTATTTGTCTACTGGAAGCGAGCTCTTGTTGGAGATTTTGGGACCTGGAATTGTTGGGGGATTGTTTCTTCCTGCGCTTGGTGCTCTTCCTGATGCTATGCTCATTCTTGTTTCTGGTCTTGCAGGAAGTGCTGAAGTCGCACAAAGCCAGGTTTCTGTGGGAATGGGCCTCTTAGCTGGTTCAACTGTCATGCTGCTCACACTCATCTGGGGCACATGTGTGATTGTTGGCAAGTGTGACCTTCAGGATTCAGTTGCCATAGATTTACAGGATACAAAAGGATTTAGCTTGACTGAATCTGGAGTGAGCACTGATATTTGGACCAGCTATGCTGCCAGGATTATGGTTATATCTGTTGTTCCATTTTTAGTAGTTCAACTTCCACAATTGCTCAATTCAACTTCAGGAAGGCACTTGGCTGTGTTGATTGCTCTTATTATCTCTGTTTCAATGTTCATTATATATTGCCTTTACCAGGTTTTCCAGCCATGGATTCAAAGGAGGAAACTTGCCTTTGTCAAGCACAAGCATGTTATATTCGGGCTCTTAAAACATTTAAAACAGCAAGCACTTGGAAGACTCCTGACAGAAAGTGGGGAGCCAGATAGAGAAATAATAAAAAAGCTATTTTCAAGAATCGATGCGAATAAGAATGGACTTCTTTCAGCTTCTGAACTGAGAGCATTAATCGTTGGCATTCAGTTTGAGGCGATCGACTTAGATCATGATGATGCTGTTAACAAAATATTGAGTGACTTCGATACTTCTCGTGATAACCATGTCGATTCTATCGAGTTTGCTAATGGGATCATTAGGTGGCTAGCTCAAGTACAAGGAGCAAGAACTGGCAGAGCTGATGATGGTCCACACACATTGAAATACCTGCACAACTTTCATCAAGAAACAAAGAGAGAGCATGATTTGCTGGATGTCGGGGAACAGAGTGACGAGGTTGTAGAGGGTGTCGAGGAGAGCAAGGGAGTGTTGATCAGAGCCATACTCTTCTTGTTGCTTGGAACAGCCATTGCTGCTGCCTTTGCCGATCCTTTGGTCGACGTTGTACATAACTTCTCCAATGCAACGAGTATCCCTGCGTTCTTCATTTCGTTTATAGCTTTGCCTTTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACCGCCTCCTTAACATTTTCTGAGTTATATGGAGCAGTGACAATGAACAATGTGCTTTGTCTATCTGTGTTCTTGGCTCTTGTTTATCTGAGAGGATTGGTTTGGAACTTTTCTTCTGAAGTATTGGTTATTCTGGTCGTTACGATGATCATGGGACTGCTCGGGAGCTTCCGAACCGCGTTCCCGCTGTGGACAGCCCTGGTGGCTCTCCTTCTCTACCCCCTGTCTCTTGTGCTGGTCTATGTCCTTGATTATGTGTTTGGTTGGTCATAG

Protein sequence

MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREIIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Homology
BLAST of CmaCh01G012350 vs. ExPASy Swiss-Prot
Match: Q8L636 (Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 3.0e-203
Identity = 386/577 (66.90%), Postives = 454/577 (78.68%), Query Frame = 0

Query: 12  LIFFLLCGQSNSSRFSSIS-SSSDLVSDGVNELQENLRLNSL--ESVSTP----EESCEE 71
           L+F L    S  +RF S++ SS+ L+SDG++         S+    VS P    EE+CE+
Sbjct: 9   LLFLLFFTSSAYARFVSLNPSSTSLISDGIDGGSNLAGSGSVIKSVVSAPAEEKEEACEQ 68

Query: 72  SYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALGALP 131
           +YGF+PCT +ALGN+FLI+VYG+LMF AA YLS GSELLLEILGPGIVGGLFLP LGALP
Sbjct: 69  TYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPMLGALP 128

Query: 132 DAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVAIDLQ 191
           DAMLI+VSGL+G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL+DS+A++ Q
Sbjct: 129 DAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIAVNNQ 188

Query: 192 DTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALIISVS 251
           DTKGF L +SGV+ DIWTSYAARIM ISV+PF++VQLPQ+L STSGR L+VLIALI+SV 
Sbjct: 189 DTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALILSVL 248

Query: 252 MFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREIIKKLF 311
           M I YC+YQVFQPWIQRR+LAF KHKHVI G+L+HLKQ ALGRLL + G+PD  +I+KLF
Sbjct: 249 MLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVIRKLF 308

Query: 312 SRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEFANGI 371
             IDAN +G LSA+EL+ALI+GI FE ID D DDAV K+L DFD + D  VD  EF  GI
Sbjct: 309 LTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRGI 368

Query: 372 IRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEG--VEESKGV 431
            +WL Q  G      + GP T+K+L NFH +TKREH LL   E  +   EG  V + K +
Sbjct: 369 KQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADPKWI 428

Query: 432 LIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAIIF 491
            I+A L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSAIIF
Sbjct: 429 TIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSAIIF 488

Query: 492 ASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMGL 551
           ASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V ++MG 
Sbjct: 489 ASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLVMGG 548

Query: 552 LGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
             SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 549 FASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of CmaCh01G012350 vs. ExPASy Swiss-Prot
Match: Q5QNI2 (Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 PE=2 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.5e-186
Identity = 353/580 (60.86%), Postives = 453/580 (78.10%), Query Frame = 0

Query: 8   LIFLLIFFLLC---GQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVS----TPEE 67
           L+ LL+F L     G+  S    + +S++ L+S+ V+ +      NS  + S     PEE
Sbjct: 10  LVPLLLFLLAAAAYGRLISDGSPASASATSLLSNPVSAVIRLTTSNSASASSPPAAAPEE 69

Query: 68  SCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL 127
            CE+SYGFLPCTT+ LGNLFL++ YG+LM+ AA +LS GSELLLEI+GPG+VGGL LP L
Sbjct: 70  KCEQSYGFLPCTTTVLGNLFLVLAYGFLMYKAATFLSAGSELLLEIMGPGLVGGLLLPIL 129

Query: 128 GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL-QDSV 187
           GALPDA+L+LVSGL+GS E AQSQV +GMGLLAGSTV LLTL+WGTCV+VGKCD+  + V
Sbjct: 130 GALPDALLVLVSGLSGSRETAQSQVLIGMGLLAGSTVFLLTLLWGTCVVVGKCDIGPNGV 189

Query: 188 AIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIAL 247
           A+DLQ+ KGFSLT +G+STD+ TSYAARIM ISV+PF++ Q P++L +  G+ LAVL+AL
Sbjct: 190 AVDLQNNKGFSLTGTGISTDVQTSYAARIMGISVIPFIIAQFPKMLKTHHGQRLAVLLAL 249

Query: 248 IISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREI 307
           I+S S+ + YCLYQVFQPWIQ+RKLA+ KHKHVI G+L+H + +ALGRLL E G P+ ++
Sbjct: 250 IVSFSLVLAYCLYQVFQPWIQKRKLAYAKHKHVISGILRHAQMEALGRLLNEDGTPNEDV 309

Query: 308 IKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIE 367
           IKKLF +ID +++  LS +EL ALI+GI FE +D D +DAV+KI+ DFDTS ++ V+  E
Sbjct: 310 IKKLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDIVEEAE 369

Query: 368 FANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEES 427
           F +G+ RWL +   A+      G ++ K++ ++H  T++EHDLL   ++SDE VE VE  
Sbjct: 370 FVSGMKRWLNE---AKRSVPTSGAYSNKFITDYHARTRQEHDLL--VDRSDETVESVENP 429

Query: 428 KGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSA 487
              + +A+  LLLG+AIAAAFADPLVD VHNFSNA+ IP+FFISFIALPLATNSSEAVSA
Sbjct: 430 GWCITKAVGLLLLGSAIAAAFADPLVDAVHNFSNASHIPSFFISFIALPLATNSSEAVSA 489

Query: 488 IIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMI 547
           IIFASR K +T+SLTFSE+YG VTMNN LCL VFLAL+Y+R L W+FSSEVL+IL+V +I
Sbjct: 490 IIFASRKKLRTSSLTFSEVYGGVTMNNTLCLGVFLALIYIRNLTWDFSSEVLIILLVCVI 549

Query: 548 MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           MGL  SFRT FPLWT LVA +LYPLSLV+VY+LD+VFGWS
Sbjct: 550 MGLFTSFRTTFPLWTCLVAYMLYPLSLVVVYILDFVFGWS 584

BLAST of CmaCh01G012350 vs. ExPASy Swiss-Prot
Match: Q6K3R5 (Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 PE=2 SV=2)

HSP 1 Score: 616.7 bits (1589), Expect = 2.7e-175
Identity = 336/550 (61.09%), Postives = 425/550 (77.27%), Query Frame = 0

Query: 36  VSDG----VNELQENLRLNSLESVSTPEESCEESYGFLPCTTSALGNLFLIIVYGYLMFL 95
           +SDG     N     LRL S  + +  +  CE SYGFLPCTT+A GNLFL++ YG+LMF 
Sbjct: 33  ISDGGNANANANASVLRLPSAAAAAGEDMGCEMSYGFLPCTTTAWGNLFLVLAYGFLMFK 92

Query: 96  AAKYLSTGSELLLEILGPGIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGL 155
           +A YLS+GSE+LL+ILGPGIVGGLFLP LGALPDA+LILVSGL+G+ EVAQSQV +GMGL
Sbjct: 93  SATYLSSGSEMLLQILGPGIVGGLFLPILGALPDALLILVSGLSGTKEVAQSQVLIGMGL 152

Query: 156 LAGSTVMLLTLIWGTCVIVGKCDL-QDSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMV 215
           LAGSTVMLLTL+WG+CV+VGKCDL ++S AID +DTKGFSL  SGVSTD  TSYAARIM 
Sbjct: 153 LAGSTVMLLTLLWGSCVVVGKCDLSENSTAIDSRDTKGFSLLGSGVSTDKQTSYAARIMA 212

Query: 216 ISVVPFLVVQLPQLLNSTSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHK 275
           IS++PF++VQ+P++    SG  + VLI LI++  + + YCLYQVFQPWIQRR+L + + K
Sbjct: 213 ISILPFIIVQIPKIFKLHSGHQITVLIGLIVAALLLLSYCLYQVFQPWIQRRRLEYTRLK 272

Query: 276 HVIFGLLKHLKQQALGRLLTESGEPDREIIKKLFSRIDANKNGLLSASELRALIVGIQFE 335
           HV+ GLL+H ++ ++GRLL + G P+  +I+KLF RID + +G L   EL+A IVGI FE
Sbjct: 273 HVMSGLLRHAQKHSIGRLLDDEGRPNVSVIEKLFHRIDQDNDGKLERGELQAFIVGINFE 332

Query: 336 AIDLDHDDAVNKILSDFDTSRDNHVDSIEFANGIIRWLAQVQGARTGRADDGPHTLKYLH 395
            ID + + A +++++DFDTSR++ ++  EF NG++RWL + +   T     G ++ K+L+
Sbjct: 333 DIDWNSNLAADQVMADFDTSRNHFIEKGEFVNGMLRWLDEAKRTVT----SGAYSKKFLN 392

Query: 396 NFHQETKREH-DLLDVGEQSDEVVEGVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVH 455
           +FH  T+ E   LLD  E+  E  +G        I+AIL LLLGTA+AAA ADPLVD VH
Sbjct: 393 DFHARTRDEQTGLLDKDEEEGE-ADG--NPTWTCIKAILLLLLGTAMAAASADPLVDAVH 452

Query: 456 NFSNATSIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLC 515
           NFSNAT IP+FFISFI +PLATNSSEAVSAIIFASR K++T SLTFSE+YG VTMNN LC
Sbjct: 453 NFSNATHIPSFFISFIVMPLATNSSEAVSAIIFASRKKKRTLSLTFSEVYGGVTMNNTLC 512

Query: 516 LSVFLALVYLRGLVWNFSSEVLVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLV 575
           L+VFLALVY+RGL W+FSSEVL+IL+V +IMGL  SFRT FPLWT  VA LLYPLSL++V
Sbjct: 513 LAVFLALVYVRGLTWDFSSEVLIILLVCIIMGLFTSFRTDFPLWTCFVAFLLYPLSLIMV 572

Query: 576 YVLDYVFGWS 580
           Y+LDY FGWS
Sbjct: 573 YILDYKFGWS 575

BLAST of CmaCh01G012350 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 8.5e-04
Identity = 41/139 (29.50%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 405 VGEQSDEVVEGVEESKGVLI-RAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFIS 464
           +GEQ +EV E  EE K +    AI +L + T   +  +  LVD +   S + ++P  FIS
Sbjct: 261 IGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFIS 320

Query: 465 FIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRG-- 524
            I LP+  N++E  SAI+FA +DK     +T     G+ T  ++  +   + + ++ G  
Sbjct: 321 VILLPIVGNAAEHASAIMFAMKDK---LDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQ 380

Query: 525 LVWNFSSEVLVILVVTMIM 541
           +  NF       L +T+++
Sbjct: 381 MDLNFQLFETATLFITVLV 396

BLAST of CmaCh01G012350 vs. ExPASy TrEMBL
Match: A0A6J1J1E5 (sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111480448 PE=4 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEE 60
           MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEE
Sbjct: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEE 60

Query: 61  SCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL 120
           SCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL
Sbjct: 61  SCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL 120

Query: 121 GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA 180
           GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA
Sbjct: 121 GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA 180

Query: 181 IDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALI 240
           IDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALI
Sbjct: 181 IDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALI 240

Query: 241 ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREII 300
           ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREII
Sbjct: 241 ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREII 300

Query: 301 KKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEF 360
           KKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEF
Sbjct: 301 KKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEF 360

Query: 361 ANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESK 420
           ANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESK
Sbjct: 361 ANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESK 420

Query: 421 GVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAI 480
           GVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAI
Sbjct: 421 GVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAI 480

Query: 481 IFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIM 540
           IFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIM
Sbjct: 481 IFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIM 540

Query: 541 GLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           GLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 GLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 579

BLAST of CmaCh01G012350 vs. ExPASy TrEMBL
Match: A0A6J1FT50 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 PE=4 SV=1)

HSP 1 Score: 1060.1 bits (2740), Expect = 3.4e-306
Identity = 571/581 (98.28%), Postives = 576/581 (99.14%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQEN--LRLNSLESVSTP 60
           MSRPPLYLIFLLIFF+LCGQS+SSRF+SISSSSDLVSDGVNELQE   LRLNSLESVS P
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASISSSSDLVSDGVNELQETPYLRLNSLESVSAP 60

Query: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120
           EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120

Query: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180

Query: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240
           VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA
Sbjct: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240

Query: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300
           LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLL+HLKQQALGRLLTESGEPDRE
Sbjct: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGEPDRE 300

Query: 301 IIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSI 360
           II+KLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDS 
Sbjct: 301 IIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 360

Query: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420
           EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE
Sbjct: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh01G012350 vs. ExPASy TrEMBL
Match: A0A6J1IGZ1 (sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE=4 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.6e-284
Identity = 526/581 (90.53%), Postives = 557/581 (95.87%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQE--NLRLNSLESVSTP 60
           MSR PL+L+FLL F +LC QS+SSRF+SISSSSDLVSDGVNELQE   LRLN+L S S P
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 60

Query: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120
           EESCE+SYGFLPCTTS LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+ S
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 180

Query: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240
           VAID QDTKGFSLTESGVSTDIWTSYAARIMVISVVPFL+VQLPQ+LNSTSGRHLAVLIA
Sbjct: 181 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300
           LIIS+SMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLL+HLKQQALGRLLTE+GEPD+E
Sbjct: 241 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 301 IIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSI 360
           II KLFSRID NK+G LSASELRALIVGIQFE +DLDH+DAV KIL+DFDTSRD+HVD+ 
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420
           EF NGII+WL+QVQG+RTGRADDGPHT+K+LHNFHQETKREHDLLDVGEQSDEVVE VE 
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVILVVT+
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh01G012350 vs. ExPASy TrEMBL
Match: A0A6J1FAG1 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111443554 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 3.1e-283
Identity = 524/581 (90.19%), Postives = 556/581 (95.70%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQE--NLRLNSLESVSTP 60
           MSR PL+L+FLL F +LC QS+SSRF+SISSSSDLVSDGVN+LQE   LRLN+L S S P
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNDLQEPSYLRLNTLPSNSAP 60

Query: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120
           EE+CE+SYGFLPCTTS LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGPGIVGGLFLP
Sbjct: 61  EEACEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+ S
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 180

Query: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240
           VAID QDTKGFSLTESGVSTDIWTSYAARIMVISV PFL+VQLPQ+LNSTSGRHLAVLIA
Sbjct: 181 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300
           LIIS+SMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLL+HLKQQALGRLLTE+GEPD+E
Sbjct: 241 LIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 301 IIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSI 360
           II KLFSRID NK+G LSASELRALIVGIQFE +DLDH+DAV KIL+DFDTSRD+HVD+ 
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420
           EF NGII+WL+QVQG+RTGRADDGPHT+K+LHNFHQETKREHDLLDVGEQSDEVVE VE 
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVILVVT+
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh01G012350 vs. ExPASy TrEMBL
Match: A0A5A7VFE4 (Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001180 PE=4 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 7.6e-282
Identity = 526/590 (89.15%), Postives = 555/590 (94.07%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSI--------SSSSDLVSDGVNELQE--NLRLN 60
           MS+ PL+L+FLL F +LC QS SSR  SI        SSSSDLVSDGVNELQE   L LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  SLESVSTP-EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120
           +L S S P EESCE+SYGFLPCTT+ALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180
           GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI
Sbjct: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180

Query: 181 VGKCDLQDSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTS 240
           VGKCDLQDSVAID QDTKGFSLTESGVSTDIWTSYAARIMVISVVPFL+VQLPQ+LNSTS
Sbjct: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 240

Query: 241 GRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLL 300
           GRHLAVLIALI+SVSMFI YCLYQVFQPWIQRRKLAFVKHKHVIFG L+HLKQQ LGRLL
Sbjct: 241 GRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLL 300

Query: 301 TESGEPDREIIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDT 360
           TE+GEPD+EII+KLFSRID NK+GLLSASELRALIVGIQF+ IDLDHDDAV+KI++DFDT
Sbjct: 301 TENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDT 360

Query: 361 SRDNHVDSIEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQS 420
           SRD+HVDS EF NGIIRWL+QVQG+RTGR DDGPHT+KYLHNFHQETKREHDLLDVGEQS
Sbjct: 361 SRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQS 420

Query: 421 DEVVEGVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPL 480
           DEVVEGVEE KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPL
Sbjct: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPL 480

Query: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540
           ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE
Sbjct: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540

Query: 541 VLVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           VLVILVVT++MG++GSFRTAFPLWT+LVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of CmaCh01G012350 vs. NCBI nr
Match: XP_022981259.1 (sodium/calcium exchanger NCL-like [Cucurbita maxima])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEE 60
           MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEE
Sbjct: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEE 60

Query: 61  SCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL 120
           SCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL
Sbjct: 61  SCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPAL 120

Query: 121 GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA 180
           GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA
Sbjct: 121 GALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVA 180

Query: 181 IDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALI 240
           IDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALI
Sbjct: 181 IDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALI 240

Query: 241 ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREII 300
           ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREII
Sbjct: 241 ISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREII 300

Query: 301 KKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEF 360
           KKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEF
Sbjct: 301 KKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEF 360

Query: 361 ANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESK 420
           ANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESK
Sbjct: 361 ANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESK 420

Query: 421 GVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAI 480
           GVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAI
Sbjct: 421 GVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAI 480

Query: 481 IFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIM 540
           IFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIM
Sbjct: 481 IFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIM 540

Query: 541 GLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           GLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 GLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 579

BLAST of CmaCh01G012350 vs. NCBI nr
Match: KAG6607902.1 (Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1060.1 bits (2740), Expect = 7.0e-306
Identity = 571/581 (98.28%), Postives = 576/581 (99.14%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQEN--LRLNSLESVSTP 60
           MSRPPLYLIFLLIFF+LCGQS+SSRF+SISSSSDLVSDGVNELQE   LRLNSLESVS P
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASISSSSDLVSDGVNELQETPYLRLNSLESVSAP 60

Query: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120
           EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120

Query: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180

Query: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240
           VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA
Sbjct: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240

Query: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300
           LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE
Sbjct: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300

Query: 301 IIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSI 360
           II+KLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDS 
Sbjct: 301 IIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 360

Query: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420
           EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQ+DEVVEGVEE
Sbjct: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 420

Query: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh01G012350 vs. NCBI nr
Match: XP_022941280.1 (sodium/calcium exchanger NCL-like [Cucurbita moschata])

HSP 1 Score: 1060.1 bits (2740), Expect = 7.0e-306
Identity = 571/581 (98.28%), Postives = 576/581 (99.14%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQEN--LRLNSLESVSTP 60
           MSRPPLYLIFLLIFF+LCGQS+SSRF+SISSSSDLVSDGVNELQE   LRLNSLESVS P
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASISSSSDLVSDGVNELQETPYLRLNSLESVSAP 60

Query: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120
           EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120

Query: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180

Query: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240
           VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA
Sbjct: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240

Query: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300
           LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLL+HLKQQALGRLLTESGEPDRE
Sbjct: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGEPDRE 300

Query: 301 IIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSI 360
           II+KLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDS 
Sbjct: 301 IIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 360

Query: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420
           EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE
Sbjct: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh01G012350 vs. NCBI nr
Match: XP_023523899.1 (sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.3e-303
Identity = 565/581 (97.25%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQEN--LRLNSLESVSTP 60
           MSRPPLYLIFLLIFFL CGQS+SSRF+SISSSSDLVSDGVNELQE   LRLNSLESVS P
Sbjct: 1   MSRPPLYLIFLLIFFLFCGQSHSSRFASISSSSDLVSDGVNELQETPYLRLNSLESVSAP 60

Query: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120
           EESCEESYGFLPCTTS LGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEESYGFLPCTTSVLGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120

Query: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180

Query: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240
           VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA
Sbjct: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240

Query: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300
           LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLL+HLKQQALGRLLTESGEPD+E
Sbjct: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGEPDKE 300

Query: 301 IIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSI 360
           II KLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRD+HVD+ 
Sbjct: 301 IIDKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDSHVDAT 360

Query: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420
           EFANGIIRWLA VQGARTGRADDGPHT+KYLHNFHQETKREHDLLDVGEQSDEVVEGVEE
Sbjct: 361 EFANGIIRWLAHVQGARTGRADDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh01G012350 vs. NCBI nr
Match: XP_022976461.1 (sodium/calcium exchanger NCL-like [Cucurbita maxima])

HSP 1 Score: 988.0 bits (2553), Expect = 3.4e-284
Identity = 526/581 (90.53%), Postives = 557/581 (95.87%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQE--NLRLNSLESVSTP 60
           MSR PL+L+FLL F +LC QS+SSRF+SISSSSDLVSDGVNELQE   LRLN+L S S P
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 60

Query: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120
           EESCE+SYGFLPCTTS LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+ S
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 180

Query: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240
           VAID QDTKGFSLTESGVSTDIWTSYAARIMVISVVPFL+VQLPQ+LNSTSGRHLAVLIA
Sbjct: 181 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDRE 300
           LIIS+SMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLL+HLKQQALGRLLTE+GEPD+E
Sbjct: 241 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 301 IIKKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSI 360
           II KLFSRID NK+G LSASELRALIVGIQFE +DLDH+DAV KIL+DFDTSRD+HVD+ 
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420
           EF NGII+WL+QVQG+RTGRADDGPHT+K+LHNFHQETKREHDLLDVGEQSDEVVE VE 
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVILVVT+
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh01G012350 vs. TAIR 10
Match: AT1G53210.1 (sodium/calcium exchanger family protein / calcium-binding EF hand family protein )

HSP 1 Score: 709.5 bits (1830), Expect = 2.2e-204
Identity = 386/577 (66.90%), Postives = 454/577 (78.68%), Query Frame = 0

Query: 12  LIFFLLCGQSNSSRFSSIS-SSSDLVSDGVNELQENLRLNSL--ESVSTP----EESCEE 71
           L+F L    S  +RF S++ SS+ L+SDG++         S+    VS P    EE+CE+
Sbjct: 9   LLFLLFFTSSAYARFVSLNPSSTSLISDGIDGGSNLAGSGSVIKSVVSAPAEEKEEACEQ 68

Query: 72  SYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALGALP 131
           +YGF+PCT +ALGN+FLI+VYG+LMF AA YLS GSELLLEILGPGIVGGLFLP LGALP
Sbjct: 69  TYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPMLGALP 128

Query: 132 DAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDSVAIDLQ 191
           DAMLI+VSGL+G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL+DS+A++ Q
Sbjct: 129 DAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIAVNNQ 188

Query: 192 DTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIALIISVS 251
           DTKGF L +SGV+ DIWTSYAARIM ISV+PF++VQLPQ+L STSGR L+VLIALI+SV 
Sbjct: 189 DTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALILSVL 248

Query: 252 MFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDREIIKKLF 311
           M I YC+YQVFQPWIQRR+LAF KHKHVI G+L+HLKQ ALGRLL + G+PD  +I+KLF
Sbjct: 249 MLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVIRKLF 308

Query: 312 SRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEFANGI 371
             IDAN +G LSA+EL+ALI+GI FE ID D DDAV K+L DFD + D  VD  EF  GI
Sbjct: 309 LTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRGI 368

Query: 372 IRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEG--VEESKGV 431
            +WL Q  G      + GP T+K+L NFH +TKREH LL   E  +   EG  V + K +
Sbjct: 369 KQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADPKWI 428

Query: 432 LIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAIIF 491
            I+A L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSAIIF
Sbjct: 429 TIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSAIIF 488

Query: 492 ASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMGL 551
           ASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V ++MG 
Sbjct: 489 ASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLVMGG 548

Query: 552 LGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 580
             SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 549 FASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of CmaCh01G012350 vs. TAIR 10
Match: AT1G29025.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 245.7 bits (626), Expect = 8.9e-65
Identity = 188/590 (31.86%), Postives = 311/590 (52.71%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSIS---SSSDLVSDGVNELQENLRLNSLESVST 60
           M+R  L+L FL +   L    N    SS+S   +SS LVSDGV +   N  L SL   + 
Sbjct: 1   MARLTLFLSFLSL--ALVSVVNCRVLSSVSEDNNSSILVSDGVQDRSSNDFL-SLNPPNL 60

Query: 61  PEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFL 120
            E +C   YGFLPC  +  G +F +  +G L+ +   +LS G   L  I   G  GG+  
Sbjct: 61  SESACVHVYGFLPCADNIEGYVFQVFSFGCLLIIGEYFLSKGRSKLFVIFEVGFFGGIIF 120

Query: 121 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ- 180
           P L   P   LIL +GL GS E+A S     + +  G +V  LT+ WG CV+ G   L+ 
Sbjct: 121 PLLTMFPRIALILSTGLIGSREIANSMTGNNVAVTVGYSVFALTMQWGACVVFGLSGLRS 180

Query: 181 --DSVAIDL-----QDTKGFSLTE----SGVSTDIWTSYAARIMVISVVPFLVVQLPQLL 240
              S+  D+     Q  K  +L +    + +  D     AA IM+++++PF++V   +  
Sbjct: 181 DDQSITCDISSPRRQVKKSINLLKNLAGASIRADPKNKKAAGIMLLTLLPFVLVTFSETF 240

Query: 241 NSTSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQAL 300
           +  S     VL+ LI S S  ++Y +Y  F    Q + L + K + ++  + KHL Q   
Sbjct: 241 HIKSWDDNMVLVTLIFSGSATVLYFVYLYFDKADQVKSLEYAKFE-LMSEVHKHL-QNFS 300

Query: 301 GRLLTESGEPDREIIKKLFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKIL 360
            + L  +G+  +E +K LF + D NK+G +  SEL+ L V       +  D ++    +L
Sbjct: 301 PQSLIRNGQLCQESLKSLFKKFDVNKDGKIQVSELKDLTVDFGVLGRVKCDINELATTLL 360

Query: 361 SDFDTSRDNHVDSIEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLD 420
           +DFD++RD  +D  EFA GI +WL Q + +  G    G           ++   +  +L 
Sbjct: 361 ADFDSNRDGEIDETEFAIGIEKWLKQYKFSFNGTESQG-----------EDIAEDDGVLK 420

Query: 421 VGEQSDEVVEGVEESKGV---LIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFF 480
           V    +++++G   +K +    ++A++ +++G  I +  A P +  +   S +  +P+F+
Sbjct: 421 V----EQLLKGCLFTKLLTKRTLKAVIEVIIGITIVSFLAMPFMMNIELLSVSAGVPSFY 480

Query: 481 ISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRG 540
           + F  +PLA N   A+SA     ++K K  S TFSE+Y  VT+NN+L +++ LA+VY+RG
Sbjct: 481 VVFAVIPLARNLKNALSAHFCRKKEKAKITSDTFSEIYRDVTLNNLLGITIILAIVYIRG 540

Query: 541 LVWNFSSEVLVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYV 572
           L W++S+EVL+I++V +I+G+    R+ +P W  ++A  LY  SL+L+Y+
Sbjct: 541 LTWDYSTEVLIIVIVGLIIGVPAYVRSTYPFWICVLAFALYFFSLILIYL 570

BLAST of CmaCh01G012350 vs. TAIR 10
Match: AT2G34030.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 234.2 bits (596), Expect = 2.7e-61
Identity = 186/588 (31.63%), Postives = 298/588 (50.68%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSIS-SSSDLVSDGVNEL--QENLRLNSLESVST 60
           M+   L+   L I  ++ G S+    S +  ++S L+SDG+++    E L L+  +S+S 
Sbjct: 1   MAGVTLFFSLLSIALIISGVSSRVLISHVPLNNSILISDGIHDALNHEFLTLDPPKSLS- 60

Query: 61  PEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFL 120
              +C   YGFLPC  +  G +F +  +G L+ +   +LS G   L  I   G  GG+  
Sbjct: 61  -RTACVHVYGFLPCADNVEGYIFQVFSFGSLLIIGDYFLSEGRSKLFVIFEVGFYGGIIF 120

Query: 121 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG----KC 180
           P L   P   L+L +GL+ S +VA S +   +GL  G TV  LT+ WG CV+      + 
Sbjct: 121 PLLTMFPRIALMLSTGLSLSRDVASSFIDDNVGLTVGHTVFSLTIQWGACVVFSITGPRS 180

Query: 181 DLQDSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHL 240
           D  D +   L+  KGF   E+ V  D     AA IM++S+ PFL+V    + +S S  H+
Sbjct: 181 DQADGLIEKLKILKGF--VEARVEADPKNKKAAGIMLLSLSPFLMVTFSAIFDSHSWSHI 240

Query: 241 AVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESG 300
            VLI LIIS S  ++Y +Y       Q + L   + + ++  + KHLK+ +   L+ + G
Sbjct: 241 IVLITLIISSSSTVVYFVYSYLDTANQEKSLDHARFE-LMSEVHKHLKRFSPKHLI-KDG 300

Query: 301 EPDREIIKKLFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKILSDFDTSRD 360
           E  +E +K LF + D NK+G +  SEL+ L + +  F  +  D ++     L DFD   D
Sbjct: 301 ELSKESLKSLFKKTDKNKDGKIQISELKDLTIELSNFGRMRYDINELAKAFLEDFDGDND 360

Query: 361 NHVDSIEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEV 420
             ++  EF  GI R L Q                   + F+ E +RE+   + G    E+
Sbjct: 361 GELEENEFEEGIARLLKQ-------------------YKFNVEDQRENQTEENGVLKLEI 420

Query: 421 VEGVEESKGVLIRAILFL---------LLGTAIAAAFADPLVDVVHNFSNATSIPAFFIS 480
                + K  L+  +L +         ++G  I    A P +  +   S +  IP+F+I 
Sbjct: 421 -----KPKKTLVTKLLSMETLIATTEVIVGILIVLFLAKPFMLNIQLLSISAGIPSFYIV 480

Query: 481 FIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLV 540
           F  +P A N    +S      +DK++ +S TFSE+Y  +TMNN+L +SV LA+VY RGL 
Sbjct: 481 FAMIPFARNLKNTLSTRFCRGKDKKRVSSNTFSEIYKDITMNNLLGMSVILAIVYTRGLT 540

Query: 541 WNFSSEVLVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYV 572
           W +S E L++++V +I+GL    R+ +P W  ++A  +Y  SL+L+Y+
Sbjct: 541 WKYSVETLIMVIVGLIIGLPIYIRSTYPFWMCVLAFAMYFFSLLLIYI 558

BLAST of CmaCh01G012350 vs. TAIR 10
Match: AT1G29020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 233.8 bits (595), Expect = 3.5e-61
Identity = 185/580 (31.90%), Postives = 294/580 (50.69%), Query Frame = 0

Query: 1   MSRPPLYLIFLLIFFLLCGQSNSSRFSSIS-SSSDLVSDGVNE--LQENLRLNSLESVST 60
           M+R  L+L FL +  L+    N    SS+S   S LVSDGV +    E L L+    ++ 
Sbjct: 1   MARLSLFLSFLSLIALI-SVVNCRVLSSLSEKDSILVSDGVQDGSSYEFLSLDPPNDIT- 60

Query: 61  PEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFL 120
            +  C   Y FLPC  +  G +F +  +G ++ +   +L+ G   L  I   G  GG+  
Sbjct: 61  -KNMCVHVYDFLPCADNVAGYVFQVFSFGCILIIGEYFLTKGRTKLFLIFEVGFYGGIIF 120

Query: 121 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQD 180
           P L   P   LIL +GL GS E+A S+V   +G+  G +V  LT+ WG C++ G    + 
Sbjct: 121 PLLTMFPRIALILSTGLVGSREMASSRVGNIIGVTVGYSVFALTIQWGACILFGLSGTKP 180

Query: 181 SVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLI 240
              ID        L+ + V  DI    +A IM+++++PF++V   ++ +S     + VLI
Sbjct: 181 EQPID--------LSAASVEADITNKKSAGIMLLTILPFILVTFSEISSSQYWNDIIVLI 240

Query: 241 ALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGEPDR 300
            LIIS S    Y  Y  F    Q + L   + + ++  + KHL Q    + L   G+ ++
Sbjct: 241 TLIISASATFAYFFYSYFDRDDQAKSLDHARFE-LMSEVHKHL-QIFSPKSLIRDGQLNQ 300

Query: 301 EIIKKLFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKILSDFDTSRDNHVD 360
           E +K+LF +IDAN +G +  +EL+ L V       +    D  V+ ++ DFD   D  +D
Sbjct: 301 ESLKRLFQKIDANGDGKIQVAELKDLTVEFGMIGRVKCHIDKLVHTLVDDFDRDGDREID 360

Query: 361 SIEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHD------LLDVGEQSD 420
             EF  GI +WL Q                 Y  +F        D      +L V +   
Sbjct: 361 EAEFEIGIKKWLNQ-----------------YKFSFDTTVPPREDQAEGVPILKVEKPEQ 420

Query: 421 EVVEGVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLA 480
            +V+ +   +   +RA L ++LG  +    A P +  +   S +  +P+F++ F+ +PLA
Sbjct: 421 SLVKKLLSWR--TLRASLEVILGMLVVIYLARPFMMNIQLLSESAGVPSFYVVFVVIPLA 480

Query: 481 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEV 540
            N    +SA     +DK K  S TFSE+Y  VTMNN++ +S+ LA+VY R LVW++++EV
Sbjct: 481 RNLKNTLSAHFCRKKDKAKITSDTFSEIYKDVTMNNLMGISIILAIVYARELVWDYTTEV 540

Query: 541 LVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVY 571
           L+I++V +++GL    RT +P W  L+A  LY +SLV+VY
Sbjct: 541 LIIVIVGLLIGLPAYVRTTYPFWICLLAFALYFISLVVVY 548

BLAST of CmaCh01G012350 vs. TAIR 10
Match: AT2G34020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 220.7 bits (561), Expect = 3.1e-57
Identity = 186/602 (30.90%), Postives = 295/602 (49.00%), Query Frame = 0

Query: 6   LYLIFLLIFFLLCGQSNSSRFSSISSSSDLVSDGVNELQENLRLNSLESVSTPEESCEES 65
           L+  FL +   + G S+         +S L+SDGV+   +   L      +  + +C   
Sbjct: 19  LFFFFLSLTLFISGVSSRVLSPVPLDNSSLISDGVHGASDYKYLTLDPPKNVSKAACIHV 78

Query: 66  YGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLPALGALPD 125
           YGFLPC  +  G +F +  +G L+ +   +LS G   L  I   G  GG+  P L   P 
Sbjct: 79  YGFLPCADNIGGYVFQVFSFGCLLIIGDYFLSQGRSKLFVIFEVGFYGGIVFPLLTMFPR 138

Query: 126 AMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD-------LQDS 185
             L++  GLA + E A   V   +G+  G T+  LT+ WG CVI G           + S
Sbjct: 139 IALMISPGLAATHEGALMIVGNNVGVTIGHTIFALTMQWGACVIFGLTSPNSDPSIRRGS 198

Query: 186 VAIDLQDTK----GF-------SLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNS 245
           +     DTK    GF       S+ E+ V  D     AA IM++++VPFL+V LP LL+ 
Sbjct: 199 IKRTSSDTKNPRRGFYRMKILKSVVEASVDADPKNKKAAGIMLLTLVPFLLVTLPDLLDV 258

Query: 246 TSGRHLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGR 305
            S   + +LI LIIS S   IY +Y  F    Q++ L   K + ++  + KHL Q    R
Sbjct: 259 QSWSDIIMLITLIISCSSTFIYFVYSYFDTADQKKSLDHAKFE-LMSEVHKHL-QSFSPR 318

Query: 306 LLTESGEPDREIIKKLFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKILSD 365
            L   G+  +E +K LF +ID NK+G +  SEL+ L V    F  +  D ++  + +L++
Sbjct: 319 TLIRDGQLSKESLKSLFDKIDRNKDGKIQISELKDLTVEFGVFGKMKCDINEFASTLLAE 378

Query: 366 FDTSRDNHVDSIEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVG 425
           FD  ++  +D  EF  GI++ L      +    +       Y++    ++ +    L  G
Sbjct: 379 FDKDKNGELDENEFEEGIMKLLNHY---KFDNQESPRQNNTYIYRTPSDSVKS---LSQG 438

Query: 426 EQSDEVVEGVEESKGVLI---------RAILFLLLGTAIAAAFADPLVDVVHNFSNATSI 485
           E++   V  +E  K  L+         RA+  ++ G  I    A P +  +   S +  +
Sbjct: 439 EEAG--VLKLEMPKQTLVAKFLSMSTLRAVTKVIGGMLIVVFLAKPFMVNIGLLSVSAGV 498

Query: 486 PAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALV 545
           P+F+  F  +PL  N    +SA     +DK + AS  FSE+Y  VTMNN++ +S+ LA+V
Sbjct: 499 PSFYSVFAVIPLVRNLKNTLSAHFCRKKDKARIASEKFSEIYRDVTMNNLMGMSITLAIV 558

Query: 546 YLRGLVWNFSSEVLVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFG 580
           Y RGL W +S E L+++VV + +GL    R+ +P W  ++A  +Y  SLVL+Y+  ++ G
Sbjct: 559 YSRGLKWEYSIESLLVVVVGIAIGLPAYVRSTYPFWICVMAFAMYIFSLVLIYIHFHLRG 610

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6363.0e-20366.90Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1[more]
Q5QNI21.5e-18660.86Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 P... [more]
Q6K3R52.7e-17561.09Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 P... [more]
Q5KQN08.5e-0429.50Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1J1E50.0e+00100.00sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111480448 PE... [more]
A0A6J1FT503.4e-30698.28sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 ... [more]
A0A6J1IGZ11.6e-28490.53sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE... [more]
A0A6J1FAG13.1e-28390.19sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111443554 ... [more]
A0A5A7VFE47.6e-28289.15Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
XP_022981259.10.0e+00100.00sodium/calcium exchanger NCL-like [Cucurbita maxima][more]
KAG6607902.17.0e-30698.28Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022941280.17.0e-30698.28sodium/calcium exchanger NCL-like [Cucurbita moschata][more]
XP_023523899.17.3e-30397.25sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo][more]
XP_022976461.13.4e-28490.53sodium/calcium exchanger NCL-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G53210.12.2e-20466.90sodium/calcium exchanger family protein / calcium-binding EF hand family protein... [more]
AT1G29025.18.9e-6531.86Calcium-binding EF-hand family protein [more]
AT2G34030.12.7e-6131.63Calcium-binding EF-hand family protein [more]
AT1G29020.13.5e-6131.90Calcium-binding EF-hand family protein [more]
AT2G34020.13.1e-5730.90Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 339..367
e-value: 66.0
score: 3.1
coord: 299..327
e-value: 0.049
score: 22.8
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 300..323
e-value: 1.6E-4
score: 21.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 295..330
score: 12.002819
IPR002048EF-hand domainCDDcd00051EFhcoord: 300..364
e-value: 4.78803E-7
score: 45.2313
NoneNo IPR availableGENE3D1.10.238.10coord: 255..374
e-value: 3.4E-10
score: 42.1
NoneNo IPR availablePANTHERPTHR31503:SF46SUBFAMILY NOT NAMEDcoord: 9..577
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 80..256
e-value: 4.1E-7
score: 30.1
coord: 427..570
e-value: 5.4E-11
score: 42.7
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 9..577
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 348..360
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 308..320
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 281..504

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G012350.1CmaCh01G012350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity