CmaCh01G010270 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G010270
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionwall-associated receptor kinase 2-like
LocationCma_Chr01: 7547175 .. 7547786 (-)
RNA-Seq ExpressionCmaCh01G010270
SyntenyCmaCh01G010270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAAGCATCACCAATGACTCCTTCATTGGAGATGCCAACATGGTTCTACATCGCCTTGATGATAATGGCGGCCATGGCCACGGACGATGAAGTCAACCGGTCAGCCATCGCCTTGCCGGGCTGCTCGTACCAATGCGGCGGAGTCGAGATTCCATATCCATTTGGACTCACTCCTGAATGCTCCCTTAATGAGGCATTTCTCGTCACTTGCAACACCTCGATTCTTCCCAACAAGCCATTCATTGAGGACATTCCGATCATGAGTGTTTCAGTGGAAGATGCTGATTTGGTTATCGAAAATCTTGTGGCTAATTATTGTTTTGATGGCAAAGGAAATATCTCCGGCCACAATGAAACGCTTCTTAAGTTTGATAAGTTCACGATCTCGACGAAGAATATCTTCACCGTCGTCGGTTGTAGTACTGTTTCGATGATCGGAGGCATCCTCCAAGATGATGAGGATTACTTGTCGGGTTGTGCTTCATTTTGTAGTAGCTATCGGAATATGCCGAACGGGACGTGTTCCGGTGTCGGGTGTTGCCAAATGACAATTCCTAGTGGATTAAAACAGGCAAGATTTTTGTTAAGAATATTTAGTATTGAGTTATAG

mRNA sequence

TTTCAAGCATCACCAATGACTCCTTCATTGGAGATGCCAACATGGTTCTACATCGCCTTGATGATAATGGCGGCCATGGCCACGGACGATGAAGTCAACCGGTCAGCCATCGCCTTGCCGGGCTGCTCGTACCAATGCGGCGGAGTCGAGATTCCATATCCATTTGGACTCACTCCTGAATGCTCCCTTAATGAGGCATTTCTCGTCACTTGCAACACCTCGATTCTTCCCAACAAGCCATTCATTGAGGACATTCCGATCATGAGTGTTTCAGTGGAAGATGCTGATTTGGTTATCGAAAATCTTGTGGCTAATTATTGTTTTGATGGCAAAGGAAATATCTCCGGCCACAATGAAACGCTTCTTAAGTTTGATAAGTTCACGATCTCGACGAAGAATATCTTCACCGTCGTCGGTTGTAGTACTGTTTCGATGATCGGAGGCATCCTCCAAGATGATGAGGATTACTTGTCGGGTTGTGCTTCATTTTGTAGTAGCTATCGGAATATGCCGAACGGGACGTGTTCCGGTGTCGGGTGTTGCCAAATGACAATTCCTAGTGGATTAAAACAGGCAAGATTTTTGTTAAGAATATTTAGTATTGAGTTATAG

Coding sequence (CDS)

TTTCAAGCATCACCAATGACTCCTTCATTGGAGATGCCAACATGGTTCTACATCGCCTTGATGATAATGGCGGCCATGGCCACGGACGATGAAGTCAACCGGTCAGCCATCGCCTTGCCGGGCTGCTCGTACCAATGCGGCGGAGTCGAGATTCCATATCCATTTGGACTCACTCCTGAATGCTCCCTTAATGAGGCATTTCTCGTCACTTGCAACACCTCGATTCTTCCCAACAAGCCATTCATTGAGGACATTCCGATCATGAGTGTTTCAGTGGAAGATGCTGATTTGGTTATCGAAAATCTTGTGGCTAATTATTGTTTTGATGGCAAAGGAAATATCTCCGGCCACAATGAAACGCTTCTTAAGTTTGATAAGTTCACGATCTCGACGAAGAATATCTTCACCGTCGTCGGTTGTAGTACTGTTTCGATGATCGGAGGCATCCTCCAAGATGATGAGGATTACTTGTCGGGTTGTGCTTCATTTTGTAGTAGCTATCGGAATATGCCGAACGGGACGTGTTCCGGTGTCGGGTGTTGCCAAATGACAATTCCTAGTGGATTAAAACAGGCAAGATTTTTGTTAAGAATATTTAGTATTGAGTTATAG

Protein sequence

FQASPMTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFDKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTIPSGLKQARFLLRIFSIEL
Homology
BLAST of CmaCh01G010270 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.1e-18
Identity = 53/156 (33.97%), Postives = 83/156 (53.21%), Query Frame = 0

Query: 42  CSYQCGGVEIPYPFGLTPECSL--NEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVI 101
           C  +CG V + YPFG +P C    +E+F +TCN      K F  ++P++++S+    L +
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQ---EKLFFGNMPVINMSL-SGQLRV 88

Query: 102 ENLVANYCFDGKGNISGHNETLLKFDKFTISTKNIFTVVGCSTVSMI--GGILQDDEDYL 161
             + +  C+D +G  + +         FT+S  N FTVVGC++ + +   G+    E Y 
Sbjct: 89  RLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSELNRFTVVGCNSYAFLRTSGV----EKYS 148

Query: 162 SGCASFCSSYRNMPNGTCSGVGCCQMTIPSGLKQAR 194
           +GC S C S     NG+CSG GCCQ+ +P G    R
Sbjct: 149 TGCISICDS-ATTKNGSCSGEGCCQIPVPRGYSFVR 175

BLAST of CmaCh01G010270 vs. ExPASy Swiss-Prot
Match: Q8GXQ3 (Wall-associated receptor kinase-like 6 OS=Arabidopsis thaliana OX=3702 GN=WAKL6 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 7.3e-11
Identity = 52/185 (28.11%), Postives = 83/185 (44.86%), Query Frame = 0

Query: 32  VNRSAIALP---------GCSYQCGGVEIPYPFGLTPECSLNEAFLVTCNTSILPNK--- 91
           +N S+ A P          C+  CGGV IP+PFG+  +C LN  + V CNTS   +    
Sbjct: 23  INGSSAATPPPPNSNSSTSCNRACGGVSIPFPFGIGKDCYLNGWYEVICNTSTSGSSGTT 82

Query: 92  -PFIEDI--PIMSVSVEDADLV-----IENLVANYCFDGKGNISGHNETLL-------KF 151
            PF+  I   ++++S+ D   +     I+  V +       + S  +E  L       + 
Sbjct: 83  VPFLSRINSEVVNISLPDGKKLYGVVHIKGPVTSLGCSSSSSSSQVSEMSLPNLNVTGRG 142

Query: 152 DKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFC---SSYRNMPNGTCSGVGCC 187
             + ++ +N   +VGC T +    +++D E  + GC S C    S   + N  C G  CC
Sbjct: 143 SPYFLTDENCLVMVGCGTKA----LMKDIESEILGCESSCEDSKSSEEVTNSKCDGYKCC 202

BLAST of CmaCh01G010270 vs. ExPASy Swiss-Prot
Match: Q9SA25 (Wall-associated receptor kinase-like 8 OS=Arabidopsis thaliana OX=3702 GN=WAKL8 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-10
Identity = 57/203 (28.08%), Postives = 91/203 (44.83%), Query Frame = 0

Query: 17  YIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNEAFLVTCNTS-- 76
           ++ +M++  +      +   +AL  CS  CG V +PYPFG+   C  N+ F + C +S  
Sbjct: 8   FLVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKSSSD 67

Query: 77  -----ILPN-KPFIEDIPI---MSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 136
                +LP  +  +    +    S+SV +   +   L  + C +  G  S  +   LK  
Sbjct: 68  QQPILLLPRIRRAVTSFNLGDPFSISVYNKFYIQSPLKHSGCPNRDGYSS--SSLNLKGS 127

Query: 137 KFTISTKNIFTVVGCSTVSM--IGGILQDDEDYLSGCASFCS----SYRNMPNGTCSGVG 196
            F IS  N FT VGC+  +   + G+       + GC + C     SY+   N +C G  
Sbjct: 128 PFFISENNKFTAVGCNNKAFMNVTGL------QIVGCETTCGNEIRSYKG-ANTSCVGYK 187

Query: 197 CCQMTIPSGLKQARFLLRIFSIE 203
           CCQMTIP  L+   F   +  +E
Sbjct: 188 CCQMTIPPLLQLQVFDATVEKLE 201

BLAST of CmaCh01G010270 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 3.6e-10
Identity = 41/142 (28.87%), Postives = 72/142 (50.70%), Query Frame = 0

Query: 42  CSYQCGGVEIPYPFGLTPECSL--NEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVI 101
           C  +CG V I YPFG++  C    ++ F +TC   ++  K  +  I  ++       + +
Sbjct: 31  CKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---VVEEKLLLFGIIQVTNISHSGHVSV 90

Query: 102 ENLVANYCFDGKGNISGHNETLLKFDKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSG 161
                + C++ K   +G          F++S+ N FT+VGC+ +S++       ++Y +G
Sbjct: 91  LFERFSECYEQKNETNGTALGYQLGSSFSLSSNNKFTLVGCNALSLLSTF--GKQNYSTG 150

Query: 162 CASFCSSYRNMPNGTCSGVGCC 182
           C S C+S +   NG C+GVGCC
Sbjct: 151 CLSLCNS-QPEANGRCNGVGCC 166

BLAST of CmaCh01G010270 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.7e-10
Identity = 53/193 (27.46%), Postives = 88/193 (45.60%), Query Frame = 0

Query: 12  MPTWFYIA-LMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSL--NEAFL 71
           M  +FY+A   ++ A   DD           C  +CG V I YPFG++  C    +++F 
Sbjct: 9   MAIFFYLAYTQLVKAQPRDD-----------CQTRCGDVPIDYPFGISTGCYYPGDDSFN 68

Query: 72  VTCNTSILPNKPFIEDIPIMSVSVEDADL----VIENLV--ANYCFDGKGNISGHNETLL 131
           +TC           ED P +  ++E  +      +  L+  +  C+D + N +       
Sbjct: 69  ITCE----------EDKPNVLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTN-NDFESLWF 128

Query: 132 KFDKFTISTKNIFTVVGCSTVSMIG--GILQDDEDYLSGCASFCSSYRNMPNGTCSGVGC 191
           + D  + S  N FT+VGC+  +++   GI    ++Y +GC S C +    PN  C+GVGC
Sbjct: 129 RLDNLSFSPNNKFTLVGCNAWALLSTFGI----QNYSTGCMSLCDT-PPPPNSKCNGVGC 174

Query: 192 CQMTIPSGLKQAR 194
           C+  +   L   R
Sbjct: 189 CRTEVSIPLDSHR 174

BLAST of CmaCh01G010270 vs. ExPASy TrEMBL
Match: A0A6J1J2T9 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111480762 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.4e-104
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0

Query: 6   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 65
           MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE
Sbjct: 1   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 60

Query: 66  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 125
           AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD
Sbjct: 61  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 120

Query: 126 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 185
           KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI
Sbjct: 121 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 180

Query: 186 PSGLKQ 192
           PSGLKQ
Sbjct: 181 PSGLKQ 186

BLAST of CmaCh01G010270 vs. ExPASy TrEMBL
Match: A0A6J1EHF8 (wall-associated receptor kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111433410 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 8.8e-100
Identity = 180/191 (94.24%), Postives = 186/191 (97.38%), Query Frame = 0

Query: 6   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 65
           MT SLEMPTWFYIA MIMAAMA DDEV+RSAIALPGCSYQCG VEIPYPFGLTPECSLNE
Sbjct: 1   MTSSLEMPTWFYIAFMIMAAMA-DDEVDRSAIALPGCSYQCGEVEIPYPFGLTPECSLNE 60

Query: 66  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 125
           AFLVTCNTSILPNKPF++DIPIMSVSVEDADLVIENLVANYCFDGKGN+SGHNETLLKFD
Sbjct: 61  AFLVTCNTSILPNKPFVDDIPIMSVSVEDADLVIENLVANYCFDGKGNMSGHNETLLKFD 120

Query: 126 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 185
           KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI
Sbjct: 121 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 180

Query: 186 PSGLKQARFLL 197
           PSGLKQA F++
Sbjct: 181 PSGLKQASFVV 190

BLAST of CmaCh01G010270 vs. ExPASy TrEMBL
Match: A0A5D3DI48 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G003160 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.5e-67
Identity = 128/192 (66.67%), Postives = 153/192 (79.69%), Query Frame = 0

Query: 2   QASPMTPSLEMPTWFYIALMIMAAMATDDEV--NRSAIALPGCSYQCGGVEIPYPFGLTP 61
           Q   MT S  M +WFYI L+I+ ++ TD++V   +  IALPGC Y+CG VEIPYPFGLTP
Sbjct: 101 QPLSMTFSWGMRSWFYILLLII-SVVTDNDVAYGQLPIALPGCLYRCGEVEIPYPFGLTP 160

Query: 62  ECSLNEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNE 121
           ECSLNEAFLVTCN S  PNKPF+ DIPI S+SV+D +L IE+ VA YCFDG GN+SG NE
Sbjct: 161 ECSLNEAFLVTCNGSFNPNKPFVGDIPITSISVDDGELGIESPVAKYCFDGDGNVSGKNE 220

Query: 122 TLLKFDKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVG 181
           T L+ ++FTIS KNI TV+GCST+S I GI Q DE+Y++ CASFCSSYRNMPNG+CSGVG
Sbjct: 221 TFLESNQFTISRKNIITVIGCSTISTITGIFQGDENYITACASFCSSYRNMPNGSCSGVG 280

Query: 182 CCQMTIPSGLKQ 192
           CCQ+TIP GL Q
Sbjct: 281 CCQVTIPGGLNQ 291

BLAST of CmaCh01G010270 vs. ExPASy TrEMBL
Match: A0A1S3BIX4 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103490390 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.5e-67
Identity = 128/192 (66.67%), Postives = 153/192 (79.69%), Query Frame = 0

Query: 2   QASPMTPSLEMPTWFYIALMIMAAMATDDEV--NRSAIALPGCSYQCGGVEIPYPFGLTP 61
           Q   MT S  M +WFYI L+I+ ++ TD++V   +  IALPGC Y+CG VEIPYPFGLTP
Sbjct: 100 QPLSMTFSWGMRSWFYILLLII-SVVTDNDVAYGQLPIALPGCLYRCGEVEIPYPFGLTP 159

Query: 62  ECSLNEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNE 121
           ECSLNEAFLVTCN S  PNKPF+ DIPI S+SV+D +L IE+ VA YCFDG GN+SG NE
Sbjct: 160 ECSLNEAFLVTCNGSFNPNKPFVGDIPITSISVDDGELGIESPVAKYCFDGDGNVSGKNE 219

Query: 122 TLLKFDKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVG 181
           T L+ ++FTIS KNI TV+GCST+S I GI Q DE+Y++ CASFCSSYRNMPNG+CSGVG
Sbjct: 220 TFLESNQFTISRKNIITVIGCSTISTITGIFQGDENYITACASFCSSYRNMPNGSCSGVG 279

Query: 182 CCQMTIPSGLKQ 192
           CCQ+TIP GL Q
Sbjct: 280 CCQVTIPGGLNQ 290

BLAST of CmaCh01G010270 vs. ExPASy TrEMBL
Match: A0A0A0K5G2 (GUB_WAK_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G219790 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.8e-66
Identity = 120/181 (66.30%), Postives = 147/181 (81.22%), Query Frame = 0

Query: 12  MPTWFYIALMIMAAMATDD-EVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNEAFLVT 71
           M +WFYI L+I++  A +D   ++  IALPGC Y+CG VEIPYPFGLTPECSLN+AFLVT
Sbjct: 1   MRSWFYILLLIISVEADNDVAYDQLPIALPGCLYRCGEVEIPYPFGLTPECSLNDAFLVT 60

Query: 72  CNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFDKFTIS 131
           CN S  PNKPF+  +PI S+SV+D +L I++ VANYCFDG GN+SG NET L+ ++FTIS
Sbjct: 61  CNDSFNPNKPFVRHVPITSISVDDGELGIKSPVANYCFDGNGNVSGKNETFLESNQFTIS 120

Query: 132 TKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTIPSGLK 191
           TKNI TV+GCST+S I G  Q +E+YL+ CASFCSSYRNMPNG+CSGVGCCQ+TIP GL 
Sbjct: 121 TKNIITVIGCSTISTISGTFQGNENYLTACASFCSSYRNMPNGSCSGVGCCQVTIPGGLN 180

BLAST of CmaCh01G010270 vs. NCBI nr
Match: XP_022981698.1 (wall-associated receptor kinase 2-like [Cucurbita maxima])

HSP 1 Score: 387.5 bits (994), Expect = 7.1e-104
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0

Query: 6   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 65
           MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE
Sbjct: 1   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 60

Query: 66  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 125
           AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD
Sbjct: 61  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 120

Query: 126 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 185
           KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI
Sbjct: 121 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 180

Query: 186 PSGLKQ 192
           PSGLKQ
Sbjct: 181 PSGLKQ 186

BLAST of CmaCh01G010270 vs. NCBI nr
Match: KAG6607701.1 (Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 372.9 bits (956), Expect = 1.8e-99
Identity = 180/191 (94.24%), Postives = 186/191 (97.38%), Query Frame = 0

Query: 6   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 65
           MT SLEMPTWFYIA MIMAAMA DDEV+RSAIALPGCSYQCG VEIPYPFGLTPECSLNE
Sbjct: 1   MTSSLEMPTWFYIAFMIMAAMA-DDEVDRSAIALPGCSYQCGEVEIPYPFGLTPECSLNE 60

Query: 66  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 125
           AFLVTCNTSILPNKPF++DIPIMSVSVEDADLVIENLVANYCFDGKGN+SGHNETLLKFD
Sbjct: 61  AFLVTCNTSILPNKPFVDDIPIMSVSVEDADLVIENLVANYCFDGKGNMSGHNETLLKFD 120

Query: 126 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 185
           KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI
Sbjct: 121 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 180

Query: 186 PSGLKQARFLL 197
           PSGLKQA F++
Sbjct: 181 PSGLKQASFVV 190

BLAST of CmaCh01G010270 vs. NCBI nr
Match: XP_022926228.1 (wall-associated receptor kinase 2-like [Cucurbita moschata])

HSP 1 Score: 372.9 bits (956), Expect = 1.8e-99
Identity = 180/191 (94.24%), Postives = 186/191 (97.38%), Query Frame = 0

Query: 6   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 65
           MT SLEMPTWFYIA MIMAAMA DDEV+RSAIALPGCSYQCG VEIPYPFGLTPECSLNE
Sbjct: 1   MTSSLEMPTWFYIAFMIMAAMA-DDEVDRSAIALPGCSYQCGEVEIPYPFGLTPECSLNE 60

Query: 66  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 125
           AFLVTCNTSILPNKPF++DIPIMSVSVEDADLVIENLVANYCFDGKGN+SGHNETLLKFD
Sbjct: 61  AFLVTCNTSILPNKPFVDDIPIMSVSVEDADLVIENLVANYCFDGKGNMSGHNETLLKFD 120

Query: 126 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 185
           KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI
Sbjct: 121 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 180

Query: 186 PSGLKQARFLL 197
           PSGLKQA F++
Sbjct: 181 PSGLKQASFVV 190

BLAST of CmaCh01G010270 vs. NCBI nr
Match: XP_023523494.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.7 bits (940), Expect = 1.3e-97
Identity = 177/186 (95.16%), Postives = 182/186 (97.85%), Query Frame = 0

Query: 6   MTPSLEMPTWFYIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNE 65
           MT SLEMPTWFYIA MIMAAMA DDEV+RSAIALPGCSYQCG VEIPYPFGLTPECSLNE
Sbjct: 1   MTSSLEMPTWFYIAFMIMAAMA-DDEVDRSAIALPGCSYQCGEVEIPYPFGLTPECSLNE 60

Query: 66  AFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 125
           AFLVTCNTSILPNKPF+++IPIMSVSVEDADLVIENLVANYCFDGKGN+SGHNETLLKFD
Sbjct: 61  AFLVTCNTSILPNKPFVDNIPIMSVSVEDADLVIENLVANYCFDGKGNMSGHNETLLKFD 120

Query: 126 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 185
           KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI
Sbjct: 121 KFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVGCCQMTI 180

Query: 186 PSGLKQ 192
           PSGLKQ
Sbjct: 181 PSGLKQ 185

BLAST of CmaCh01G010270 vs. NCBI nr
Match: TYK23263.1 (wall-associated receptor kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 265.8 bits (678), Expect = 3.1e-67
Identity = 128/192 (66.67%), Postives = 153/192 (79.69%), Query Frame = 0

Query: 2   QASPMTPSLEMPTWFYIALMIMAAMATDDEV--NRSAIALPGCSYQCGGVEIPYPFGLTP 61
           Q   MT S  M +WFYI L+I+ ++ TD++V   +  IALPGC Y+CG VEIPYPFGLTP
Sbjct: 101 QPLSMTFSWGMRSWFYILLLII-SVVTDNDVAYGQLPIALPGCLYRCGEVEIPYPFGLTP 160

Query: 62  ECSLNEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVIENLVANYCFDGKGNISGHNE 121
           ECSLNEAFLVTCN S  PNKPF+ DIPI S+SV+D +L IE+ VA YCFDG GN+SG NE
Sbjct: 161 ECSLNEAFLVTCNGSFNPNKPFVGDIPITSISVDDGELGIESPVAKYCFDGDGNVSGKNE 220

Query: 122 TLLKFDKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFCSSYRNMPNGTCSGVG 181
           T L+ ++FTIS KNI TV+GCST+S I GI Q DE+Y++ CASFCSSYRNMPNG+CSGVG
Sbjct: 221 TFLESNQFTISRKNIITVIGCSTISTITGIFQGDENYITACASFCSSYRNMPNGSCSGVG 280

Query: 182 CCQMTIPSGLKQ 192
           CCQ+TIP GL Q
Sbjct: 281 CCQVTIPGGLNQ 291

BLAST of CmaCh01G010270 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 94.0 bits (232), Expect = 1.5e-19
Identity = 53/156 (33.97%), Postives = 83/156 (53.21%), Query Frame = 0

Query: 42  CSYQCGGVEIPYPFGLTPECSL--NEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVI 101
           C  +CG V + YPFG +P C    +E+F +TCN      K F  ++P++++S+    L +
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQ---EKLFFGNMPVINMSL-SGQLRV 88

Query: 102 ENLVANYCFDGKGNISGHNETLLKFDKFTISTKNIFTVVGCSTVSMI--GGILQDDEDYL 161
             + +  C+D +G  + +         FT+S  N FTVVGC++ + +   G+    E Y 
Sbjct: 89  RLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSELNRFTVVGCNSYAFLRTSGV----EKYS 148

Query: 162 SGCASFCSSYRNMPNGTCSGVGCCQMTIPSGLKQAR 194
           +GC S C S     NG+CSG GCCQ+ +P G    R
Sbjct: 149 TGCISICDS-ATTKNGSCSGEGCCQIPVPRGYSFVR 175

BLAST of CmaCh01G010270 vs. TAIR 10
Match: AT1G16110.1 (wall associated kinase-like 6 )

HSP 1 Score: 68.9 bits (167), Expect = 5.2e-12
Identity = 52/185 (28.11%), Postives = 83/185 (44.86%), Query Frame = 0

Query: 32  VNRSAIALP---------GCSYQCGGVEIPYPFGLTPECSLNEAFLVTCNTSILPNK--- 91
           +N S+ A P          C+  CGGV IP+PFG+  +C LN  + V CNTS   +    
Sbjct: 23  INGSSAATPPPPNSNSSTSCNRACGGVSIPFPFGIGKDCYLNGWYEVICNTSTSGSSGTT 82

Query: 92  -PFIEDI--PIMSVSVEDADLV-----IENLVANYCFDGKGNISGHNETLL-------KF 151
            PF+  I   ++++S+ D   +     I+  V +       + S  +E  L       + 
Sbjct: 83  VPFLSRINSEVVNISLPDGKKLYGVVHIKGPVTSLGCSSSSSSSQVSEMSLPNLNVTGRG 142

Query: 152 DKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSGCASFC---SSYRNMPNGTCSGVGCC 187
             + ++ +N   +VGC T +    +++D E  + GC S C    S   + N  C G  CC
Sbjct: 143 SPYFLTDENCLVMVGCGTKA----LMKDIESEILGCESSCEDSKSSEEVTNSKCDGYKCC 202

BLAST of CmaCh01G010270 vs. TAIR 10
Match: AT1G16260.1 (Wall-associated kinase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-11
Identity = 57/203 (28.08%), Postives = 91/203 (44.83%), Query Frame = 0

Query: 17  YIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNEAFLVTCNTS-- 76
           ++ +M++  +      +   +AL  CS  CG V +PYPFG+   C  N+ F + C +S  
Sbjct: 8   FLVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKSSSD 67

Query: 77  -----ILPN-KPFIEDIPI---MSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 136
                +LP  +  +    +    S+SV +   +   L  + C +  G  S  +   LK  
Sbjct: 68  QQPILLLPRIRRAVTSFNLGDPFSISVYNKFYIQSPLKHSGCPNRDGYSS--SSLNLKGS 127

Query: 137 KFTISTKNIFTVVGCSTVSM--IGGILQDDEDYLSGCASFCS----SYRNMPNGTCSGVG 196
            F IS  N FT VGC+  +   + G+       + GC + C     SY+   N +C G  
Sbjct: 128 PFFISENNKFTAVGCNNKAFMNVTGL------QIVGCETTCGNEIRSYKG-ANTSCVGYK 187

Query: 197 CCQMTIPSGLKQARFLLRIFSIE 203
           CCQMTIP  L+   F   +  +E
Sbjct: 188 CCQMTIPPLLQLQVFDATVEKLE 201

BLAST of CmaCh01G010270 vs. TAIR 10
Match: AT1G16260.2 (Wall-associated kinase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-11
Identity = 57/203 (28.08%), Postives = 91/203 (44.83%), Query Frame = 0

Query: 17  YIALMIMAAMATDDEVNRSAIALPGCSYQCGGVEIPYPFGLTPECSLNEAFLVTCNTS-- 76
           ++ +M++  +      +   +AL  CS  CG V +PYPFG+   C  N+ F + C +S  
Sbjct: 8   FLVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKSSSD 67

Query: 77  -----ILPN-KPFIEDIPI---MSVSVEDADLVIENLVANYCFDGKGNISGHNETLLKFD 136
                +LP  +  +    +    S+SV +   +   L  + C +  G  S  +   LK  
Sbjct: 68  QQPILLLPRIRRAVTSFNLGDPFSISVYNKFYIQSPLKHSGCPNRDGYSS--SSLNLKGS 127

Query: 137 KFTISTKNIFTVVGCSTVSM--IGGILQDDEDYLSGCASFCS----SYRNMPNGTCSGVG 196
            F IS  N FT VGC+  +   + G+       + GC + C     SY+   N +C G  
Sbjct: 128 PFFISENNKFTAVGCNNKAFMNVTGL------QIVGCETTCGNEIRSYKG-ANTSCVGYK 187

Query: 197 CCQMTIPSGLKQARFLLRIFSIE 203
           CCQMTIP  L+   F   +  +E
Sbjct: 188 CCQMTIPPLLQLQVFDATVEKLE 201

BLAST of CmaCh01G010270 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 66.6 bits (161), Expect = 2.6e-11
Identity = 41/142 (28.87%), Postives = 72/142 (50.70%), Query Frame = 0

Query: 42  CSYQCGGVEIPYPFGLTPECSL--NEAFLVTCNTSILPNKPFIEDIPIMSVSVEDADLVI 101
           C  +CG V I YPFG++  C    ++ F +TC   ++  K  +  I  ++       + +
Sbjct: 31  CKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---VVEEKLLLFGIIQVTNISHSGHVSV 90

Query: 102 ENLVANYCFDGKGNISGHNETLLKFDKFTISTKNIFTVVGCSTVSMIGGILQDDEDYLSG 161
                + C++ K   +G          F++S+ N FT+VGC+ +S++       ++Y +G
Sbjct: 91  LFERFSECYEQKNETNGTALGYQLGSSFSLSSNNKFTLVGCNALSLLSTF--GKQNYSTG 150

Query: 162 CASFCSSYRNMPNGTCSGVGCC 182
           C S C+S +   NG C+GVGCC
Sbjct: 151 CLSLCNS-QPEANGRCNGVGCC 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMP12.1e-1833.97Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q8GXQ37.3e-1128.11Wall-associated receptor kinase-like 6 OS=Arabidopsis thaliana OX=3702 GN=WAKL6 ... [more]
Q9SA252.1e-1028.08Wall-associated receptor kinase-like 8 OS=Arabidopsis thaliana OX=3702 GN=WAKL8 ... [more]
Q9LMN83.6e-1028.87Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMN74.7e-1027.46Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1J2T93.4e-104100.00wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1114807... [more]
A0A6J1EHF88.8e-10094.24wall-associated receptor kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A5D3DI481.5e-6766.67Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BIX41.5e-6766.67wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103490390 P... [more]
A0A0A0K5G22.8e-6666.30GUB_WAK_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G21979... [more]
Match NameE-valueIdentityDescription
XP_022981698.17.1e-104100.00wall-associated receptor kinase 2-like [Cucurbita maxima][more]
KAG6607701.11.8e-9994.24Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022926228.11.8e-9994.24wall-associated receptor kinase 2-like [Cucurbita moschata][more]
XP_023523494.11.3e-9795.16wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo][more]
TYK23263.13.1e-6766.67wall-associated receptor kinase 2-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G21270.11.5e-1933.97wall-associated kinase 2 [more]
AT1G16110.15.2e-1228.11wall associated kinase-like 6 [more]
AT1G16260.11.5e-1128.08Wall-associated kinase family protein [more]
AT1G16260.21.5e-1128.08Wall-associated kinase family protein [more]
AT1G21240.12.6e-1128.87wall associated kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 42..83
e-value: 6.6E-8
score: 32.9
NoneNo IPR availablePANTHERPTHR33491OSJNBA0016N04.9 PROTEINcoord: 17..193
NoneNo IPR availablePANTHERPTHR33491:SF5OSJNBA0016N04.9 PROTEINcoord: 17..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G010270.1CmaCh01G010270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity